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Gene Dmel\peb

General Information
Symbol	Dmel\peb	Species	D. melanogaster
Name	pebbled	Annotation symbol	CG12212
Feature type	protein_coding_gene	FlyBase ID	FBgn0003053
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	hnt
Genomic Location
Chromosome (arm)	X	Recombination map	1-7
Cytogenetic map	4C10-4C10	Sequence location	X:4,511,602..4,520,256 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene pebbled is referred to in FlyBase by the symbol Dmel\peb (CG12212, FBgn0003053). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: sequence-specific DNA binding transcription factor activity. There is experimental evidence for 13 unique biological process terms, many of which group under: anatomical structure development; biological regulation; multicellular organismal process; sensory organ development; embryonic development via the syncytial blastoderm; open tracheal system development; tissue homeostasis; cellular component organization or biogenesis; ommatidial rotation; anterior/posterior axis specification. 26 alleles are reported. The phenotypes of these alleles are annotated with: organ system; cuticle; cephalopharyngeal skeleton; extended germ band embryo; organ system subdivision; taenidial fold; external compound sense organ; embryonic/larval tracheal system; pharyngeal sclerite; compound cell cluster organ; gland; rhabdomere; spiracle; late extended germ band embryo. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Zinc finger, C2H2; Zinc finger, C2H2-like; Zinc finger, C2H2-type/integrase, DNA-binding. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-18 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult midgut, larval/adult hindgut, adult heart, adult fat body, larval salivary gland, larval trachea, adult spermathecae, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1622949_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of peb. Gene sequence location is X:4511602..4520256.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	hnt: hindsight Homozygous embryonic lethal; no germ band retraction; embryo U-shaped with head facing posteriorly. Germ line clones obtained; no maternal effect (Perrimon, Engstrom, and Mahowald, 1989, Genetics 121: 333-52). peb: pebbled Eyes markedly rough at 28-30, slightly rough (like S) at 25, and wild type at 19. RK2 (28-30).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	Sequence features HFA18817 BKN27575 BKN46212
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 4C10-4C10   Limits computationally determined from genome sequence between P{EP}CG2930EP1352 and P{EP}EP425
Experimentally Determined Cytological Location
Cytogenetic map Notes References 4C5-4C6     (Yip, 1997.1.21) 4C5-4C6   (determined by in situ hybridisation)   (Rorth, 1996) 4C5-4C6   (determined by in situ hybridisation)   (Yip and Fisher, 1991) 4C5-4C6   (determined by in situ hybridisation)   (Yip and Lipshitz, 1992)
Experimentally Determined Recombination Data
Location	1-7 (Tearle and Nusslein-Volhard, 1987) 1-7.5+ (Schalet, 1986)
Left of (cM)	cx (Schalet, 1986)
Right of (cM)	rb (Schalet, 1986)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\peb for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) peb-RA FBtr0070680  NM_057326   6458   1894 peb-RB FBtr0100265  NM_001014722   6453   1894
Additional Transcript Data & Comments
Reported size (kB)	6.2 (longest cDNA) (Yip et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein peb-PA   FBpp0070648   205.8   1894   6.21     NP_476674   AAF45960 peb-PB   FBpp0099653   205.8   1894   6.21     NP_001014722   AAX52476
Additional Polypeptide Data & Comments
Reported size (kDa)	1920 (aa) (Yip et al., 1997)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, C2H2-type/integrase, DNA-binding (IPR013087) Zinc finger, C2H2-like (IPR015880) Zinc finger, C2H2 (IPR007087)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014298 AAF45960   AE014298 AAX52476   AI114190   AY058335 AAL13564   BF506195   BI213827   BI215099   BI215339   BI581376   BI582156   BI582159   BI582332   BI589216   BI611388   BI625265   CK605324   CO188211   CO324404   EL883681   EV577019   GH941602   GH941649   GH942153   GH943938   GH944493   GH948055   GH949073   GH949688   GH950501   GH952173   GH954029   GH961257   GH961743   GH962210   GH962793   GH965785   GH966751   GH970408   GH973433   GH974354   GH977695   GH979755   GH981052   GH983096   GH983599   GH983942   GH984783   U86010 AAB95640
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O46205 O77275 Q7K3V3 Q9W4J1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 5 -- 17   amnioserosa | presumptive   (Yip et al., 1997)
Additional Descriptive Data
	peb transcript is detected starting at stage 5 of embryogenesis, in the posterior midgut primordium and in the presumptive amnioserosa, and continues to be detected in these tissues past germ band retraction. (Yip et al., 1997)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 6 -- 17   amnioserosa | presumptive   (Yip et al., 1997) embryonic stage 8 -- 17   anterior midgut primordium   (Yip et al., 1997) embryonic stage 10 -- 14   amnioserosa   • nucleus (Reed et al., 2001, Wilk et al., 2000) embryonic stage 11 -- 13   tracheal pit   • nucleus (Wilk et al., 2000) embryonic stage 11 -- 14   tracheal primordium   • nucleus (Wilk et al., 2000) embryonic stage 11 -- 17   presumptive embryonic/larval peripheral nervous system   (Yip et al., 1997) embryonic/larval tracheal system   (Yip et al., 1997) embryonic stage 12   CNS glial cell   (Yip et al., 1997) PNS glial cell   (Yip et al., 1997) embryonic stage 13   amnioserosa   • nucleus (Lamka and Lipshitz, 1999) anterior midgut primordium   • nucleus (Lamka and Lipshitz, 1999) posterior midgut primordium   • nucleus (Lamka and Lipshitz, 1999) embryonic stage 14   endoderm   (Weiss et al., 2001) presumptive embryonic/larval tracheal system   • nucleus (Wilk et al., 2000) embryonic/larval oenocyte precursor   • nucleus (Wilk et al., 2000) embryonic stage 15   embryonic/larval peripheral nervous system   • nucleus (Wilk et al., 2000) embryonic stage 16   embryonic/larval dorsal branch   • nucleus (Wilk et al., 2000)
Additional Descriptive Data
	peb protein localizes to the nucleus. (Yip et al., 1997)
Marker for
	endoderm amnioserosa
Subcellular Localization
CV Term	nucleus (Yip et al., 1997)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0003053    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0003053 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-18 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0003053 expression level Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr    509 embryo 04-06hr    1610 embryo 06-08hr    3384 embryo 08-10hr    2694 embryo 10-12hr    2266 embryo 12-14hr    1886 embryo 14-16hr    1932 embryo 16-18hr    1196 embryo 18-20hr    1025 embryo 20-22hr    865 embryo 22-24hr    697 larva L1    716 larva L2    551 larva L3 12hr old    342 larva L3 puffstage 1-2    287 larva L3 puffstage 3-6    654 larva L3 puffstage 7-9    658 white prepupae new    678 white prepupae 12hr    564 white prepupae 24hr    558 pupae 2d postWPP    368 pupae 3d postWPP    408 pupae 4d postWPP    462 adult male 01day    687 adult male 05day    862 adult male 30day    769 adult female 01day    942 adult female 05day    661 adult female 30day    634 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (209) embryo 02-04hr  (509) embryo 04-06hr  (1610) embryo 06-08hr  (3384) embryo 08-10hr  (2694) embryo 10-12hr  (2266) embryo 12-14hr  (1886) embryo 14-16hr  (1932) embryo 16-18hr  (1196) embryo 18-20hr  (1025) embryo 20-22hr  (865) embryo 22-24hr  (697) larva L1  (716) larva L2  (551) larva L3 12hr old  (342) larva L3 puffstage 1-2  (287) larva L3 puffstage 3-6  (654) larva L3 puffstage 7-9  (658) white prepupae new  (678) white prepupae 12hr  (564) white prepupae 24hr  (558) pupae 2d postWPP  (368) pupae 3d postWPP  (408) pupae 4d postWPP  (462) adult male 01day  (687) adult male 05day  (862) adult male 30day  (769) adult female 01day  (942) adult female 05day  (661) adult female 30day  (634) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr    509 embryo 04-06hr  (1610) embryo 06-08hr  (3384) embryo 08-10hr  (2694) embryo 10-12hr  (2266) embryo 12-14hr  (1886) embryo 14-16hr  (1932) embryo 16-18hr    1196 embryo 18-20hr    1025 embryo 20-22hr    865 embryo 22-24hr    697 larva L1    716 larva L2    551 larva L3 12hr old    342 larva L3 puffstage 1-2    287 larva L3 puffstage 3-6    654 larva L3 puffstage 7-9    658 white prepupae new    678 white prepupae 12hr    564 white prepupae 24hr    558 pupae 2d postWPP    368 pupae 3d postWPP    408 pupae 4d postWPP    462 adult male 01day    687 adult male 05day    862 adult male 30day    769 adult female 01day    942 adult female 05day    661 adult female 30day    634 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr    509 embryo 04-06hr    1610 embryo 06-08hr    3384 embryo 08-10hr    2694 embryo 10-12hr    2266 embryo 12-14hr    1886 embryo 14-16hr    1932 embryo 16-18hr    1196 embryo 18-20hr    1025 embryo 20-22hr    865 embryo 22-24hr    697 larva L1    716 larva L2    551 larva L3 12hr old    342 larva L3 puffstage 1-2    287 larva L3 puffstage 3-6    654 larva L3 puffstage 7-9    658 white prepupae new    678 white prepupae 12hr    564 white prepupae 24hr    558 pupae 2d postWPP    368 pupae 3d postWPP    408 pupae 4d postWPP    462 adult male 01day    687 adult male 05day    862 adult male 30day    769 adult female 01day    942 adult female 05day    661 adult female 30day    634 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr    509 embryo 04-06hr    1610 embryo 06-08hr    3384 embryo 08-10hr    2694 embryo 10-12hr    2266 embryo 12-14hr    1886 embryo 14-16hr    1932 embryo 16-18hr    1196 embryo 18-20hr    1025 embryo 20-22hr    865 embryo 22-24hr    697 larva L1    716 larva L2    551 larva L3 12hr old    342 larva L3 puffstage 1-2    287 larva L3 puffstage 3-6    654 larva L3 puffstage 7-9    658 white prepupae new    678 white prepupae 12hr    564 white prepupae 24hr    558 pupae 2d postWPP    368 pupae 3d postWPP    408 pupae 4d postWPP    462 adult male 01day    687 adult male 05day    862 adult male 30day    769 adult female 01day    942 adult female 05day    661 adult female 30day    634 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0003053 expression level Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr    509 embryo 04-06hr    1610 embryo 06-08hr    3384 embryo 08-10hr    2694 embryo 10-12hr    2266 embryo 12-14hr    1886 embryo 14-16hr    1932 embryo 16-18hr    1196 embryo 18-20hr    1025 embryo 20-22hr    865 embryo 22-24hr    697 larva L1    716 larva L2    551 larva L3 12hr old    342 larva L3 puffstage 1-2    287 larva L3 puffstage 3-6    654 larva L3 puffstage 7-9    658 white prepupae new    678 white prepupae 12hr    564 white prepupae 24hr    558 pupae 2d postWPP    368 pupae 3d postWPP    408 pupae 4d postWPP    462 adult male 01day    687 adult male 05day    862 adult male 30day    769 adult female 01day    942 adult female 05day    661 adult female 30day    634 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (209) embryo 02-04hr  (509) embryo 04-06hr  (1610) embryo 06-08hr  (3384) embryo 08-10hr  (2694) embryo 10-12hr  (2266) embryo 12-14hr  (1886) embryo 14-16hr  (1932) embryo 16-18hr  (1196) embryo 18-20hr  (1025) embryo 20-22hr  (865) embryo 22-24hr  (697) larva L1  (716) larva L2  (551) larva L3 12hr old  (342) larva L3 puffstage 1-2  (287) larva L3 puffstage 3-6  (654) larva L3 puffstage 7-9  (658) white prepupae new  (678) white prepupae 12hr  (564) white prepupae 24hr  (558) pupae 2d postWPP  (368) pupae 3d postWPP  (408) pupae 4d postWPP  (462) adult male 01day  (687) adult male 05day  (862) adult male 30day  (769) adult female 01day  (942) adult female 05day  (661) adult female 30day  (634) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr    509 embryo 04-06hr  1610 embryo 06-08hr  (3384) embryo 08-10hr  (2694) embryo 10-12hr  2266 embryo 12-14hr  1886 embryo 14-16hr  1932 embryo 16-18hr    1196 embryo 18-20hr    1025 embryo 20-22hr    865 embryo 22-24hr    697 larva L1    716 larva L2    551 larva L3 12hr old    342 larva L3 puffstage 1-2    287 larva L3 puffstage 3-6    654 larva L3 puffstage 7-9    658 white prepupae new    678 white prepupae 12hr    564 white prepupae 24hr    558 pupae 2d postWPP    368 pupae 3d postWPP    408 pupae 4d postWPP    462 adult male 01day    687 adult male 05day    862 adult male 30day    769 adult female 01day    942 adult female 05day    661 adult female 30day    634 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr    509 embryo 04-06hr    1610 embryo 06-08hr    3384 embryo 08-10hr    2694 embryo 10-12hr    2266 embryo 12-14hr    1886 embryo 14-16hr    1932 embryo 16-18hr    1196 embryo 18-20hr    1025 embryo 20-22hr    865 embryo 22-24hr    697 larva L1    716 larva L2    551 larva L3 12hr old    342 larva L3 puffstage 1-2    287 larva L3 puffstage 3-6    654 larva L3 puffstage 7-9    658 white prepupae new    678 white prepupae 12hr    564 white prepupae 24hr    558 pupae 2d postWPP    368 pupae 3d postWPP    408 pupae 4d postWPP    462 adult male 01day    687 adult male 05day    862 adult male 30day    769 adult female 01day    942 adult female 05day    661 adult female 30day    634 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr    509 embryo 04-06hr    1610 embryo 06-08hr    3384 embryo 08-10hr    2694 embryo 10-12hr    2266 embryo 12-14hr    1886 embryo 14-16hr    1932 embryo 16-18hr    1196 embryo 18-20hr    1025 embryo 20-22hr    865 embryo 22-24hr    697 larva L1    716 larva L2    551 larva L3 12hr old    342 larva L3 puffstage 1-2    287 larva L3 puffstage 3-6    654 larva L3 puffstage 7-9    658 white prepupae new    678 white prepupae 12hr    564 white prepupae 24hr    558 pupae 2d postWPP    368 pupae 3d postWPP    408 pupae 4d postWPP    462 adult male 01day    687 adult male 05day    862 adult male 30day    769 adult female 01day    942 adult female 05day    661 adult female 30day    634 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0003053    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0003053 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult midgut, larval/adult hindgut, adult heart, adult fat body, larval salivary gland, larval trachea, adult spermathecae, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0003053 expression level Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    272.2 Larval Hindgut    101.1 Larval Malpighian Tubules no informative data Larval Fat Body    4.9 Larval Salivary Gland    203.6 Larval Trachea    274.25 Larval Carcass no informative data Adult Head    38.2 Adult Eye    303.675 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    9.8 Adult Midgut    285.8 Adult Hindgut    101.4 Adult Malpighian Tubules no informative data Adult Fat Body    158 Adult Salivary Gland no informative data Adult Heart    286.25 Adult VirginFemale Spermatheca    166.4 Adult InseminatedFemale Spermatheca    208.2 Adult Ovary    48.4 Adult Testis    58.6 Adult Male Accessory Gland    1.8 Adult Carcass    122 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    272.2 Larval Hindgut    101.1 Larval Malpighian Tubules no informative data Larval Fat Body    4.9 Larval Salivary Gland    203.6 Larval Trachea    274.25 Larval Carcass no informative data Adult Head    38.2 Adult Eye    303.675 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    9.8 Adult Midgut    285.8 Adult Hindgut    101.4 Adult Malpighian Tubules no informative data Adult Fat Body    158 Adult Salivary Gland no informative data Adult Heart    286.25 Adult VirginFemale Spermatheca    166.4 Adult InseminatedFemale Spermatheca    208.2 Adult Ovary    48.4 Adult Testis    58.6 Adult Male Accessory Gland    1.8 Adult Carcass    122 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    272.2 Larval Hindgut    101.1 Larval Malpighian Tubules no informative data Larval Fat Body    4.9 Larval Salivary Gland    203.6 Larval Trachea    274.25 Larval Carcass no informative data Adult Head    38.2 Adult Eye    303.675 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    9.8 Adult Midgut    285.8 Adult Hindgut    101.4 Adult Malpighian Tubules no informative data Adult Fat Body    158 Adult Salivary Gland no informative data Adult Heart    286.25 Adult VirginFemale Spermatheca    166.4 Adult InseminatedFemale Spermatheca    208.2 Adult Ovary    48.4 Adult Testis    58.6 Adult Male Accessory Gland    1.8 Adult Carcass    122 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    272.2 Larval Hindgut    101.1 Larval Malpighian Tubules no informative data Larval Fat Body    4.9 Larval Salivary Gland    203.6 Larval Trachea    274.25 Larval Carcass no informative data Adult Head    38.2 Adult Eye    303.675 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    9.8 Adult Midgut    285.8 Adult Hindgut    101.4 Adult Malpighian Tubules no informative data Adult Fat Body    158 Adult Salivary Gland no informative data Adult Heart    286.25 Adult VirginFemale Spermatheca    166.4 Adult InseminatedFemale Spermatheca    208.2 Adult Ovary    48.4 Adult Testis    58.6 Adult Male Accessory Gland    1.8 Adult Carcass    122 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0003053 expression level Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    272.2 Larval Hindgut    101.1 Larval Malpighian Tubules no informative data Larval Fat Body    4.9 Larval Salivary Gland    203.6 Larval Trachea    274.25 Larval Carcass no informative data Adult Head    38.2 Adult Eye    303.675 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    9.8 Adult Midgut    285.8 Adult Hindgut    101.4 Adult Malpighian Tubules no informative data Adult Fat Body    158 Adult Salivary Gland no informative data Adult Heart    286.25 Adult VirginFemale Spermatheca    166.4 Adult InseminatedFemale Spermatheca    208.2 Adult Ovary    48.4 Adult Testis    58.6 Adult Male Accessory Gland    1.8 Adult Carcass    122 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    272.2 Larval Hindgut    101.1 Larval Malpighian Tubules no informative data Larval Fat Body    4.9 Larval Salivary Gland    203.6 Larval Trachea    274.25 Larval Carcass no informative data Adult Head    38.2 Adult Eye    303.675 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    9.8 Adult Midgut    285.8 Adult Hindgut    101.4 Adult Malpighian Tubules no informative data Adult Fat Body    158 Adult Salivary Gland no informative data Adult Heart    286.25 Adult VirginFemale Spermatheca    166.4 Adult InseminatedFemale Spermatheca    208.2 Adult Ovary    48.4 Adult Testis    58.6 Adult Male Accessory Gland    1.8 Adult Carcass    122 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    272.2 Larval Hindgut    101.1 Larval Malpighian Tubules no informative data Larval Fat Body    4.9 Larval Salivary Gland    203.6 Larval Trachea    274.25 Larval Carcass no informative data Adult Head    38.2 Adult Eye    303.675 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    9.8 Adult Midgut    285.8 Adult Hindgut    101.4 Adult Malpighian Tubules no informative data Adult Fat Body    158 Adult Salivary Gland no informative data Adult Heart    286.25 Adult VirginFemale Spermatheca    166.4 Adult InseminatedFemale Spermatheca    208.2 Adult Ovary    48.4 Adult Testis    58.6 Adult Male Accessory Gland    1.8 Adult Carcass    122 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    272.2 Larval Hindgut    101.1 Larval Malpighian Tubules no informative data Larval Fat Body    4.9 Larval Salivary Gland    203.6 Larval Trachea    274.25 Larval Carcass no informative data Adult Head    38.2 Adult Eye    303.675 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    9.8 Adult Midgut    285.8 Adult Hindgut    101.4 Adult Malpighian Tubules no informative data Adult Fat Body    158 Adult Salivary Gland no informative data Adult Heart    286.25 Adult VirginFemale Spermatheca    166.4 Adult InseminatedFemale Spermatheca    208.2 Adult Ovary    48.4 Adult Testis    58.6 Adult Male Accessory Gland    1.8 Adult Carcass    122 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut     Larval Hindgut     Larval Malpighian Tubules no informative data Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass no informative data Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules no informative data Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    38.2   NA  Eye    303.675   NA  Brain    9.8   no informative data  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    6.8   NA  Crop    9.8   272.2  Midgut    285.8   101.1  Hindgut    101.4   no informative data  Malpighian Tubules    no informative data   4.9  Fat Body    158   203.6  Salivary Gland    no informative data   NA  Heart    286.25   274.25  Trachea    NA   NA  VirginFemale Spermatheca    166.4   NA  InseminatedFemale Spermatheca    208.2   NA  Ovary    48.4   NA  Testis    58.6   NA  Male Accessory Gland    1.8   no informative data  Carcass    122 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_31
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003053 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12212-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for peb Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal peb2 peb3 lethal, with Scer\GAL432B pebEP55 lethal, with Scer\GAL4h-1J3 pebEP55 lethal, with Scer\GAL4how-24B pebEP55 lethal, with Scer\GAL4hs.2sev pebEP55 lethal, with Scer\GAL4T80 pebEP55 lethal | embryonic stage | partially | recessive peb308 lethal | embryonic stage | recessive pebEH704a pebhnt-1 pebhnt-E8 pebX001 lethal | recessive peb2 peb3 pebD43 pebI15 pebK32 pebPG37 pebPL67 pebZ30 semi-lethal, with Scer\GAL469B pebEP55 viable peb1 viable, with Scer\GAL4pnr-MD237 pebGD1507 Other Phenotypes Allele planar polarity defective peb1 planar polarity defective | somatic clone pebunspecified visible, with Scer\GAL469B pebEP55 visible (with peb1) pebhnt-1 visible (with pebhnt-1) peb1 visible | heat sensitive peb1 visible | somatic clone pebEH704a pebhnt-1 pebX001 wild-type pebhs.PY Phenotype manifest in Allele amnioserosa pebhnt-1 pebhnt-E8 amnioserosa | somatic clone pebEH704a pebX001 pebhnt-1 cephalopharyngeal skeleton pebhnt-E8 cephalopharyngeal skeleton | embryonic stage pebX001 cuticle pebhnt-E8 dorsal bridge pebhnt-E8 dorsal closure embryo pebEH704a pebX001 pebhnt-1 pebhnt-E8 dorsal closure embryo | somatic clone pebEH704a pebX001 pebhnt-1 embryonic/first instar larval cuticle pebEH704a embryonic/first instar larval cuticle | dorsal peb308 pebEH704a pebX001 embryonic/larval dorsal trunk peb1142 pebhnt-1 embryonic/larval tracheal system peb1142 pebhnt-1 embryonic/larval tracheal system | posterior peb1142 pebhnt-1 embryonic head pebhnt-E8 extended germ band embryo peb308 pebEH704a pebX001 pebhnt-1 extended germ band embryo | anterior pebEH704a extended germ band embryo | somatic clone pebEH704a pebX001 pebhnt-1 eye, with Scer\GAL469B pebEP55 eye (with peb1) pebhnt-1 eye (with pebhnt-1) peb1 eye | heat sensitive peb1 eye | somatic clone pebEH704a pebX001 pebhnt-1 eye photoreceptor cell | somatic clone pebhnt-1 pebunspecified filzkorper pebhnt-E8 five-cell ommatidial precursor cluster | somatic clone pebhnt-1 germ band peb1142 pebhnt-1 interommatidial bristle peb1 ommatidial precursor cluster | heat sensitive peb1 ommatidial precursor cluster | somatic clone pebX001 pebhnt-1 pebunspecified ommatidium | heat sensitive peb1 rhabdomere peb1 rhabdomere | somatic clone pebunspecified salivary gland pebD43 pebunspecified taenidial fold peb1142 pebhnt-1 taenidium peb1142 pebhnt-1 vertical bridge pebhnt-E8
Classical Alleles ( 20 )
For All Classical Alleles Show
Allele of peb Class Mutagen Stocks Known lesion pebhnt-E8 amorphic allele - genetic evidence 2 -- peb1 2 -- pebEP55 wild-type allele P-element activity 2 Yes peb1142 0 -- peb11D 0 -- peb2 spontaneous 0 -- peb308 P-element activity 0 Yes peb3 spontaneous 0 -- peb70 0 -- pebD43 ethyl methanesulfonate 0 -- pebEH704a ethyl methanesulfonate 0 -- pebhnt-1 ethyl methanesulfonate 0 -- pebhnt-E6 0 -- pebI15 ethyl methanesulfonate 0 -- pebK32 ethyl methanesulfonate 0 -- pebPG37 P-element activity Delta2-3 0 -- pebPL67 Delta2-3 0 -- pebunspecified 0 -- pebX001 0 Yes pebZ30 ethyl methanesulfonate 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of peb Class Mutagen Stocks Known lesion pebGD1507 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pebHMS00843 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pebHMS00894 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pebJF03162 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pebKK107575 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pebhs.PY in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)A113 (Harbecke and Lengyel, 1995) Df(1)bi-D2 (Oliver et al., 1988, Mevel-Ninio et al., 1995) Df(1)bi-DL3 (Oliver et al., 1988) Df(1)bi-DL5 (Oliver et al., 1988) Df(1)BSC532 (Christensen et al., 2008.6.11) Df(1)BSC580 (Christensen et al., 2008.9.3) Df(1)BSC657 (Christensen et al., 2008.12.28) Df(1)cho19 (Steinmann-Zwicky, 1988) Df(1)cho23 (Steinmann-Zwicky, 1988) Df(1)EH587 (Eberl and Hilliker, 1988, Salz, 1992, Dick et al., 1996, Yip et al., 1997) Df(1)GA56 (Oliver et al., 1988) Df(1)HC244 (Steinmann-Zwicky, 1988, Oliver et al., 1988) Df(1)HF366 (Craymer and Roy, 1980, Oliver et al., 1988) Df(1)ovo6 (Oliver et al., 1987, Oliver et al., 1988) Df(1)ovo7 (Oliver et al., 1987, Oliver et al., 1988) Df(1)ovo15 (Steinmann-Zwicky, 1988) Df(1)ovo41 (Steinmann-Zwicky, 1988) Df(1)ovo44 (Steinmann-Zwicky, 1988) Df(1)rb1 (Oliver et al., 1988) Df(1)rb13 (Steinmann-Zwicky, 1988, Oliver et al., 1988) Df(1)rb14 (Steinmann-Zwicky, 1988) Df(1)rb23 (Steinmann-Zwicky, 1988) Df(1)rb29 (Steinmann-Zwicky, 1988) Df(1)rb30 (Oliver et al., 1988) Df(1)rb33 (Oliver et al., 1988) Df(1)rb34 (Steinmann-Zwicky, 1988) Df(1)rb35 (Steinmann-Zwicky, 1988) Df(1)rb46 (Oliver et al., 1988) Df(1)rb47 (Oliver et al., 1988) Df(1)rb48 (Steinmann-Zwicky, 1988) Df(1)RC40 (Tearle and Nusslein-Volhard, 1987, Steinmann-Zwicky, 1988, Oliver et al., 1988) Df(1)Sp(rb) (Schalet, 1986) Df(1)wm258-44
Not disrupted in	Df(1)C149 (Oliver et al., 1988) Df(1)GA102 (Oliver et al., 1988) Df(1)JC70 (Schalet, 1986, Tearle and Nusslein-Volhard, 1987, Oliver et al., 1988) Df(1)N73 (Oliver et al., 1988) Df(1)rb27 (Steinmann-Zwicky, 1988) Df(1)rb42 (Steinmann-Zwicky, 1988) Df(1)rb44 (Steinmann-Zwicky, 1988) Df(1)rb46 (Yip et al., 1997) Df(1)rb50 (Steinmann-Zwicky, 1988) Tp(1;2)rb+71g (Wieschaus et al., 1984) Tp(1;2)w+64b (Wieschaus et al., 1984)
Not duplicated in	Dp(1;2;Y)w+ (Eberl and Hilliker, 1988) Dp(1;2)sn+72d (Eberl and Hilliker, 1988) Dp(1;2)w-ec (Schalet, 1986) Dp(1;3)sn13a1 (Schalet, 1986)
Duplicated in	Dp(1;2)rb+71g (Eberl and Hilliker, 1988) Dp(1;2)w+64b (Eberl and Hilliker, 1988)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1507} NA P{KK107575} NA P{TRiP.HMS00843} NA P{TRiP.HMS00894} NA P{TRiP.JF03162} NA heat-shock construct P{hs-peb.Y} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}pebEP55 NA insertion of enhancer trap P{lacW}l(1)G0273G0273 No P{lacW}l(1)G0288G0288 No P{lacW}l(1)G0304G0304 No P{lacW}pebPL67 No miscellaneous insertions P{hsp70-boss}peb308 NA insertion of enhancer trap binary system P{GawB}pebPG37 No
Gene Ontology: Function, Process & Cellular Component ( 22 unique terms )
Terms Based on Experimental Evidence ( 13 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
amnioserosa maintenance inferred from mutant phenotype (Frank and Rushlow, 1996) compound eye development inferred from mutant phenotype (Pickup et al., 2002) dorsal closure inferred from mutant phenotype (Reed et al., 2001) establishment of ommatidial planar polarity inferred from mutant phenotype (Pickup et al., 2002) germ-band shortening inferred from mutant phenotype (Frank and Rushlow, 1996, Goldman-Levi et al., 1996, Lamka and Lipshitz, 1999) maintenance of epithelial integrity, open tracheal system inferred from mutant phenotype (Wilk et al., 2000) negative regulation of JNK cascade inferred from genetic interaction with bsk AND inferred from genetic interaction with dpp (Reed et al., 2001) ommatidial rotation inferred from mutant phenotype (Pickup et al., 2002) open tracheal system development inferred from expression pattern (Wilk et al., 2000) photoreceptor cell morphogenesis inferred from mutant phenotype (Pickup et al., 2002) terminal region determination inferred from genetic interaction with tor (Strecker et al., 1991) torso signaling pathway inferred from genetic interaction with tor (Strecker et al., 1991) wing disc dorsal/ventral pattern formation inferred from genetic interaction with dally (Han et al., 2005)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 12 terms )
Molecular Function
CV term Evidence References
nucleic acid binding inferred from electronic annotation with InterPro:IPR013087 (FlyBase Curators et al., 2004-) sequence-specific DNA binding transcription factor activity inferred from sequence or structural similarity with EMBL:AF013754 (FlyBase, 1992-) zinc ion binding inferred from electronic annotation with InterPro:IPR007087, InterPro:IPR015880 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
amnioserosa maintenance traceable author statement (Stronach and Perrimon, 2001, Harden, 2002, Raftery and Sutherland, 2003) dorsal closure traceable author statement (Harden, 2002) dorsal closure, leading edge cell fate determination non-traceable author statement (Stronach and Perrimon, 2001) ectoderm development traceable author statement (Raftery and Sutherland, 2003) germ-band shortening traceable author statement (Reed et al., 2001, Harden, 2002) head involution non-traceable author statement (Frank and Rushlow, 1996) regulation of transcription, DNA-dependent inferred from sequence or structural similarity with EMBL:AF013754 (FlyBase, 1992-) non-traceable author statement (Martin and Wood, 2002) regulation of tube architecture, open tracheal system traceable author statement (Ghabrial et al., 2003)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with EMBL:AF013754 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions peb allele Gene References peb1 Acsl (Wilk et al., 2004) aop (Wilk et al., 2004) aPKC (Wilk et al., 2004) apt (Wilk et al., 2004) babo (Wilk et al., 2004) blw (Wilk et al., 2004) bsk (Pickup et al., 2002) cact (Wilk et al., 2004) Cg25C (Wilk et al., 2004) chic (Wilk et al., 2004) chn (Wilk et al., 2004) Coprox (Wilk et al., 2004) CycE (Wilk et al., 2004) dac (Wilk et al., 2004) Dl (Wilk et al., 2004) Dmn (Wilk et al., 2004) dom (Wilk et al., 2004) dve (Wilk et al., 2004) ed (Wilk et al., 2004) Egfr (Wilk et al., 2004) eIF-4a (Wilk et al., 2004) eRF1 (Wilk et al., 2004) fs(2)neo12 (Wilk et al., 2004) gl (Wilk et al., 2004) heix (Wilk et al., 2004) Hel25E (Wilk et al., 2004) Jra (Pickup et al., 2002, Wilk et al., 2004) kst (Wilk et al., 2004) l(2)00297 (Wilk et al., 2004) l(2)03605 (Wilk et al., 2004) l(2)k00605b (Wilk et al., 2004) l(2)k00611 (Wilk et al., 2004) l(2)k06617 (Wilk et al., 2004) l(2)k10001 (Wilk et al., 2004) l(2)k10113 (Wilk et al., 2004) l(2)k13211 (Wilk et al., 2004) l(2)k13905 (Wilk et al., 2004) l(2)k15826 (Wilk et al., 2004) l(2)s1878 (Wilk et al., 2004) l(3)L3930 (Wilk et al., 2004) l(3)neo51 (Wilk et al., 2004) MESK2 (Wilk et al., 2004) ms(3)neo94 (Wilk et al., 2004) not (Wilk et al., 2004) osa (Wilk et al., 2004) Pdsw (Wilk et al., 2004) ps (Wilk et al., 2004) puc (Wilk et al., 2004) px (Wilk et al., 2004) rept (Wilk et al., 2004) retn (Wilk et al., 2004) Rho1 (Wilk et al., 2004) rols (Wilk et al., 2004) Rpn6 (Wilk et al., 2004) Rya-r44F (Wilk et al., 2004) Sec61α (Wilk et al., 2004) Sec61β (Wilk et al., 2004) Thiolase (Wilk et al., 2004) vri (Wilk et al., 2004) peb308 Cg25C (Wilk et al., 2004) chic (Wilk et al., 2004) Dl (Wilk et al., 2004) dpp (Reed et al., 2001) kst (Wilk et al., 2004) LanA (Wilk et al., 2004) Rho1 (Wilk et al., 2004) pebEH704a InR (Lamka and Lipshitz, 1999) pebhnt-1 InR (Lamka and Lipshitz, 1999) srp (Goldman-Levi et al., 1996) ush (Goldman-Levi et al., 1996) unspecified slbo (Wilk et al., 2004) tkv (Wilk et al., 2004) tor (Strecker et al., 1992) tutl (Wilk et al., 2004) unch (Wilk et al., 2004) vkg (Wilk et al., 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 57908 DroID - A comprehensive database of gene and protein interactions. • FBgn0003053 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31391
Orthologs
Genome-wide drosophilid orthologs	Dana\GF19359 Dere\GG18715 Dgri\GH24554 Dmoj\GI16234 Dper\GL16423 Dpse\GA11481 Dvir\GJ15926 Dwil\GK16116 Dyak\GE16354
Curated drosophilid orthologs	Dpse\GA11481   Drep\peb
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL010501-PA Aedes aegypti (mosquito) AGAP000984-PA Anopheles gambiae (mosquito) XP_001120500.1 Apis mellifera (honey bee) XP_001942902.1 Acyrthosiphon pisum (pea aphid) At3g48430.1 Arabidopsis thaliana (wall cress) 268999 Branchiostoma floridae (lancelet) BGIBMGA004355-PA Bombyx mori (silk worm) ENSBTAP00000042051 Bos taurus (bovine) CAL0003780 Candida albicans (a form of yeast) ENSCAFP00000014006 Canis familiaris (dog) CPIJ017756 Culex pipens (mosquito) ENSCPOP00000012954 Cavia porcellus (guinea pig) RP45778 Caenorhabditis remanei (nematode) DDB0220662 Dictyostelium discoideum (amoeba) ENSDARP00000062562 Danio rerio (zebra fish) ENSECAP00000015792 Equus caballus (horse) ENSGACP00000000675 Gymnopilus aculeatus (mushroom) ENSGALP00000020854 Gallus gallus (chicken) ENSP00000369265 Homo sapiens (human) ISCW009132-PA Ixodes scapularis (deer tick) ENSMODP00000012384 Monodelphis domestica (gray opossum) ENSMMUP00000010323 Macaca mulatta (rhesus monkey) ENSMUSP00000054946 Mus musculus (house mouse) XP_001603756.1 Nasonia vitripennis (wasp) ENSORLP00000004122 Oryzias latipes (japanese killifish) PHUM003860-PA Pediculus humanus (human body louse) ENSPPYP00000018124 Pongo pygmaeus (orangutan) ENSPTRP00000050621 Pan troglodytes (chimpanzee) ENSRNOP00000021007 Rattus norvegicus (norway rat) Smp_086750 Schistosoma mansoni (trematode parasite) XP_001177842.1 Strongylocentrotus purpuratus (purple sea urchin) XP_973372.2 Tribolium castaneum (red flour beetle) ENSTNIP00000007627 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000015675 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000053079 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV10071 LH11416 ISCW009132 FBgn0233884 FBgn0218118 FBgn0203113 FBgn0167292 FBgn0167291 JGI_V11_45911 FBgn0154028 FBgn0138983 FBgn0136160 FBgn0132010 FBgn0110924 FBgn0096369 CPIJ017756 BGIBMGA004355 ACYPI005235 GB18410 AGAP000984 PB21411 PHUM551540 PHUM551880 GLEAN_09560
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	80 peb1 v1 3241 y1 pebhnt-E8/FM7c 5358 w1118 P{EP}pebEP55 28735 y1 v1; P{TRiP.JF03162}attP2 33901 y1 sc* v1; P{TRiP.HMS00843}attP2 33943 y1 sc* v1; P{TRiP.HMS00894}attP2/TM3, Sb1
Kyoto	105720 peb1 v1 106936 y1 pebhnt-E8/FM7c 108183 w1118 P{EP}pebEP55
VDRC	v101325 P{KK107575}VIE-260B v3788 w1118; P{GD1507}v3788
Genomic Clones ( 1 )
	BACR38L01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 44 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH10905
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT08603 RE19437 RE21315 RE21718 RH17587 RH18761 RH20107 RH20110 RH20315 RH31579 RH65577 UT01843
Other clones	Dmel_dig_018342_0659_1307 EK038421 EK193602 IP13335 RP004113916 RP004120715 RP004149260 RP004171668 RP004181689 RP004189528 RP004202093 RP004204550 RP004208089 RP004213600 RP004235308 RP004241404 RP004247637 RP004247855 RP004258889 RP004264567 RP004265507 RP004282884 RP004365361 RP004382544 RP004427463 RP004475389 RP004487593 RP004521294 RP004528234 RP004532100 RP004536988
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003053 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31391
Antibody Information
	monoclonal, polyclonal (Yip et al., 1997)
Other Information
Discoverer
	Dubinin.
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	"l(1)G0288" may affect "peb". "l(1)G0273" may affect "peb". "l(1)G0304" may affect "peb". (Peter et al., 2002)
Other Comments
	peb has many functions during eye development. peb is required immediately posterior to the morphogenetic furrow for the correct morphology and arrangement of cells in the ommatidial precluster (which contains the R8, R2, R5, R3 and R4 precursors). peb is also required in the outer photoreceptor cells for establishment of correct chirality and the rotation of ommatidia. peb is required to maintain ommatidial structure and integrity during the mid-pupal stage. peb is required in each photoreceptor cell to accomplish apical-basal extension of the cell and its rhabdomere during the pupal period. peb is required for the separation of neighbouring rhabdomeres that occurs during the late pupal period. (Pickup et al., 2002) peb is a tissue specific factor that may regulate maintenance of the tracheal epithelium as well as differentiation of taenidia. (Wilk et al., 2000) Kr is activated by peb in the amnioserosa. (Lamka and Lipshitz, 1999) In peb mutants the amnioserosa undergoes premature cell death. (Lamka and Lipshitz, 1999) peb activity in the amnioserosa, particularly in those cells adjacent to the epidermis, is necessary for germ band retraction. (Lamka and Lipshitz, 1999) peb has been cloned and characterised. (Yip et al., 1997) A member of the ush-group of genes which are required for maintenance of the amnioserosa once it has differentiated. (Frank and Rushlow, 1996) Mutants affect amnioserosa differentiation as well as germ band retraction. (Frank and Rushlow, 1995) peb gene encodes a putative zinc finger transcription factor expressed in specific glial in the developing CNS. Lack of peb protein alters the migration of axons which grow across the glia. (Lamka and Lipshitz, 1995) Muscle phenotype of mutants studied using polarised light microscopy and antibody staining to detect Mhc-lacZ reporter gene expression in muscles. (Drysdale et al., 1993) Mutations in peb generate malformations of the longitudinal tracts. (Jacobs, 1993) Zygotically active locus involved in the terminal developmental program in the embryo. (Strecker et al., 1991) Mutations of the zygotic loci peb specifically affect germband retraction. (Yip and Lipshitz, 1991) A screen for X-linked genes that affect embryo morphology revealed peb. (Eberl and Hilliker, 1988) peb mutants display no germ band retraction, embryos are 'U-shaped' with a head facing the posterior end. (Wieschaus et al., 1984)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057326 NM_001014722 RefSeq (Proteins) • NP_476674 NP_001014722 DDBJ / EMBL / GenBank • AAB95640 AAF45960 AAF45960.1 AAL13564 AAX52476 AI114190 AL031227 AY058335 U86010 Entrez Gene - A searchable database of RefSeq genes. • 31391 UniProtKB/TrEMBL • O46205 O77275 Q7K3V3 Q9W4J1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, C2H2-type/integrase, DNA-binding (IPR013087) Zinc finger, C2H2-like (IPR015880) Zinc finger, C2H2 (IPR007087)
Linkouts
	BioGRID - A database of protein and genetic interactions • 57908 DroID - A comprehensive database of gene and protein interactions. • FBgn0003053 DRSC - Results from RNAi screens. • FBgn0003053 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003053 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12212-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003053 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31391 InParanoid A subset of ortholog calls from InParanoid. • AAEL010501-PA Aedes aegypti (mosquito) AGAP000984-PA Anopheles gambiae (mosquito) XP_001120500.1 Apis mellifera (honey bee) XP_001942902.1 Acyrthosiphon pisum (pea aphid) At3g48430.1 Arabidopsis thaliana (wall cress) 268999 Branchiostoma floridae (lancelet) BGIBMGA004355-PA Bombyx mori (silk worm) ENSBTAP00000042051 Bos taurus (bovine) CAL0003780 Candida albicans (a form of yeast) ENSCAFP00000014006 Canis familiaris (dog) CPIJ017756 Culex pipens (mosquito) ENSCPOP00000012954 Cavia porcellus (guinea pig) RP45778 Caenorhabditis remanei (nematode) DDB0220662 Dictyostelium discoideum (amoeba) ENSDARP00000062562 Danio rerio (zebra fish) ENSECAP00000015792 Equus caballus (horse) ENSGACP00000000675 Gymnopilus aculeatus (mushroom) ENSGALP00000020854 Gallus gallus (chicken) ENSP00000369265 Homo sapiens (human) ISCW009132-PA Ixodes scapularis (deer tick) ENSMODP00000012384 Monodelphis domestica (gray opossum) ENSMMUP00000010323 Macaca mulatta (rhesus monkey) ENSMUSP00000054946 Mus musculus (house mouse) XP_001603756.1 Nasonia vitripennis (wasp) ENSORLP00000004122 Oryzias latipes (japanese killifish) PHUM003860-PA Pediculus humanus (human body louse) ENSPPYP00000018124 Pongo pygmaeus (orangutan) ENSPTRP00000050621 Pan troglodytes (chimpanzee) ENSRNOP00000021007 Rattus norvegicus (norway rat) Smp_086750 Schistosoma mansoni (trematode parasite) XP_001177842.1 Strongylocentrotus purpuratus (purple sea urchin) XP_973372.2 Tribolium castaneum (red flour beetle) ENSTNIP00000007627 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000015675 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000053079 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /newgene/hindst1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31391 modMine - Data generated by the modENCODE project. • FBgn0003053 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV10071 LH11416 ISCW009132 FBgn0233884 FBgn0218118 FBgn0203113 FBgn0167292 FBgn0167291 JGI_V11_45911 FBgn0154028 FBgn0138983 FBgn0136160 FBgn0132010 FBgn0110924 FBgn0096369 CPIJ017756 BGIBMGA004355 ACYPI005235 GB18410 AGAP000984 PB21411 PHUM551540 PHUM551880 GLEAN_09560
Synonyms & Secondary IDs ( 17 )
Reported As
Symbol Synonym	anon-4Cg   CG12212 (Bourbon et al., 2002, Quinones-Coello, 2007, Krejcí et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2010.12.1) EG:66A1.1   EP55 (Reed et al., 2001, ) Hind (Andrenacci et al., 2004) hnd (Ross et al., 2001) hnt (Levine and Davidson, 2005, Wilk et al., 2004, Ghabrial et al., 2003, Wilk et al., 2004, Beller et al., 2002, Reim et al., 2003, Beller et al., 2002, Martin and Wood, 2002, Pickup et al., 2002, Reed et al., 2001, Lammel and Saumweber, 2000, Reed et al., 2001, Ashe et al., 2000, Wilk et al., 2000, Pickup and Lipshitz, 2000, Lamka and Lipshitz, 1999, Lamka, 1999, Wilk and Lipshitz, 1999, Scherer and Duffy, 1999, Reed et al., 1999, Reed et al., 1998, Lamka and Lipshitz, 1998, Sigrist and Lehner, 1997, Goldman-Levi et al., 1996, Martinez Arias, 1993, Tearle and Nusslein-Volhard, 1987, Wieschaus et al., 1984, Melani and Montell, 2006, Scuderi and Letsou, 2005, Stathopoulos and Levine, 2005, Ming et al., 2008, Fernández et al., 2007, Sun and Deng, 2007, Paladi and Tepass, 2004, Melani et al., 2008, Miles et al., 2008, Wang et al., 2008, Pickup et al., 2009, Krejcí et al., 2009, Brodu et al., 2004, Windler and Bilder, 2010, Oliva and Sierralta, 2010, Vaccari et al., 2010) Hnt (Castro et al., 2005, Han et al., 2005, Gomes and Schweisguth, 2005, Lai, 2003, Koelzer and Klein, 2003, Sun et al., 2006, Sun and Deng, 2007, Rives et al., 2006, Genevet et al., 2010, Yu et al., 2008, Richardson and Pichaud, 2010, Vaccari et al., 2008, Sun et al., 2008, Tian and Deng, 2008, Chang et al., 2008, Polesello and Tapon, 2007, Meignin et al., 2007, Rajan et al., 2009, Urbano et al., 2009, Yan et al., 2009, Li et al., 2009, Zúñiga et al., 2009, Yu et al., 2010, Poulton et al., 2011, Yan et al., 2011) HNT (Reed et al., 2004, Brown and Castelli-Gair Hombria, 2000, Ming et al., 2007) PEB (Boube et al., 2000) peb (Reeves and Posakony, 2005, Bilen et al., 2006, Negre et al., 2006, Quinones-Coello, 2007, Christensen et al., 2008.6.11, Christensen et al., 2008.9.3, Christensen et al., 2008.12.28, Wang et al., 2008, Krejcí et al., 2009, Weake et al., 2011, Buffin and Gho, 2010) Peb (Raja et al., 2010) unnamed (Rorth, 1996)
Name Synonym	hindsight (Larsen et al., 2003, Paul et al., 2003, Giraldez et al., 2002, Jacinto and Martin, 2001, Stronach and Perrimon, 2001, Auld, 1999, Martin and Wood, 2002, Wilk et al., 2000, Yip, 1997.1.21, Lamka, 1999, Wieschaus et al., 1984, Melani and Montell, 2006, Ming et al., 2008, Fernández et al., 2007, Bogdan et al., 2005, Paladi and Tepass, 2004, Melani et al., 2008, Wang et al., 2008, Pickup et al., 2009, Krejcí et al., 2009, Windler and Bilder, 2010, Oliva and Sierralta, 2010, Vaccari et al., 2010) Hindsight (Jacinto et al., 2002, Weiss et al., 2001, Tepass et al., 2001, Sun et al., 2006, Sun and Deng, 2007, Rives et al., 2006, Genevet et al., 2010, Yu et al., 2008, Pocha et al., 2011, Sun and Deng, 2007, Vaccari et al., 2008, Sun et al., 2008, Tian and Deng, 2008, Polesello and Tapon, 2007, Meignin et al., 2007, Rusten et al., 2006, Rajan et al., 2009, Urbano et al., 2009, Yan et al., 2009, Li et al., 2009, Ayyar et al., 2007, Zúñiga et al., 2009, Yu et al., 2010, Poulton et al., 2011) pebbled (Negre et al., 2006, Krejcí et al., 2009, Windler and Bilder, 2010, Buffin and Gho, 2010, Oliva and Sierralta, 2010, Vaccari et al., 2010) Pebbled (Pickup et al., 2009, Poulton et al., 2011)
Secondary FlyBase IDs
	FBgn0001209 FBgn0019888
References ( 166 )
Generate a list of	All references relating to this gene ( 166 )
List References by type	Research paper  ( 105 ) Supplementary material  ( 5 ) Review  ( 15 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 24 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Book  ( 2 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 15 )
	Kazama et al., 2011, J. Neurosci. 31(21): 7619--7630 Cell Death Triggers Olfactory Circuit Plasticity via Glial Signaling in Drosophila. [FBrf0213815] Nagel and Wilson, 2011, Nat. Neurosci. 14(2): 208--216 Biophysical mechanisms underlying olfactory receptor neuron dynamics. [FBrf0212901] Pocha et al., 2011, Curr. Biol. 21(13): 1111--1117 Retromer controls epithelial cell polarity by trafficking the apical determinant crumbs. [FBrf0214250] Poulton et al., 2011, Development 138(9): 1737--1745 The microRNA pathway regulates the temporal pattern of Notch signaling in Drosophila follicle cells. [FBrf0213494] Weake et al., 2011, Genes Dev. 25(14): 1499--1509 Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation. [FBrf0214372] Yan et al., 2011, Development 138(9): 1697--1703 Drosophila PI4KIIIalpha is required in follicle cells for oocyte polarization and Hippo signaling. [FBrf0213488] Buffin and Gho, 2010, PLoS ONE 5(2): e9285 Laser microdissection of sensory organ precursor cells of Drosophila microchaetes. [FBrf0210039] Genevet et al., 2010, Dev. Cell 18(2): 300--308 Kibra is a regulator of the Salvador/Warts/Hippo signaling network. [FBrf0211094] Oliva and Sierralta, 2010, Dev. Biol. 344(2): 911--921 Regulation of axonal development by the nuclear protein hindsight (pebbled) in the Drosophila visual system. [FBrf0211428] Pitsouli and Perrimon, 2010, Development 137(21): 3615--3624 Embryonic multipotent progenitors remodel the Drosophila airways during metamorphosis. [FBrf0212055] Raja et al., 2010, Mol. Cell 38(6): 827--841 The Nonspecific Lethal Complex Is a Transcriptional Regulator in Drosophila. [FBrf0211222] Richardson and Pichaud, 2010, Development 137(4): 641--650 Crumbs is required to achieve proper organ size control during Drosophila head development. [FBrf0209872] Vaccari et al., 2010, Development 137(11): 1825--1832 The vacuolar ATPase is required for physiological as well as pathological activation of the Notch receptor. [FBrf0210791] Windler and Bilder, 2010, Curr. Biol. 20(6): 538--543 Endocytic internalization routes required for delta/notch signaling. [FBrf0210409] Yu et al., 2010, Dev. Cell 18(2): 288--299 Kibra Functions as a Tumor Suppressor Protein that Regulates Hippo Signaling in Conjunction with Merlin and Expanded. [FBrf0210017] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...