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Gene Dmel\Pfk

General Information
Symbol	Dmel\Pfk	Species	D. melanogaster
Name	Phosphofructokinase	Annotation symbol	CG4001
Feature type	protein_coding_gene	FlyBase ID	FBgn0003071
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	l(2)06339
Genomic Location
Chromosome (arm)	2R	Recombination map	2-85.2
Cytogenetic map	46E8-46F1	Sequence location	2R:5,997,245..6,004,962 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Phosphofructokinase is referred to in FlyBase by the symbol Dmel\Pfk (CG4001, FBgn0003071). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: 6-phosphofructokinase activity. There is experimental evidence that it is involved in the biological process: glycolysis. 13 alleles are reported. No phenotypic data is available. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: 6-phosphofructokinase, eukaryotic type; Phosphofructokinase; Phosphofructokinase domain; Phosphofructokinase, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed during early larval stages, during late pupal stages, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult hindgut, adult heart, adult fat body, adult salivary gland, adult spermathecae, adult testis, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult midgut, larval hindgut, larval/adult Malpighian tubules, larval fat body, larval salivary gland, larval trachea, adult ovary, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1634739_a_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Pfk. Gene sequence location is 2R:5997245..6004962.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 46E8-46F1   Limits computationally determined from genome sequence between P{PZ}14-3-3ζ07103 and P{PZ}Pfk06339&P{PZ}CCS03221
Experimentally Determined Cytological Location
Cytogenetic map Notes References 46F1-46F2   (determined by in situ hybridisation)   (Spradling et al., 1999) 46D-46E   (determined by in situ hybridisation)   (Currie and Sullivan, 1994) 46F1-46F2     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2-85.2
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Pfk for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters. Gene model reviewed during 5.46 Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Pfk-RA FBtr0088420  NM_078952   3217   950 Pfk-RB FBtr0088422  NM_165746   3508   788 Pfk-RC FBtr0088421  NM_165747   3188   788 Pfk-RD FBtr0304925  NM_001259305   3217   950 Pfk-RE FBtr0333067  NM_001273918   3133   922
Additional Transcript Data & Comments
Reported size (kB)	3.25, 3.0, 2.85 (northern blot) (Currie and Sullivan, 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Pfk-PA   FBpp0087506   105.2   950   8.65     NP_523676   AAF58840 Pfk-PB   FBpp0087508   86.6   788   6.81     NP_724890   AAF58841 Pfk-PC   FBpp0087507   86.7   788   6.74     NP_724891   AAM71065 Pfk-PD   FBpp0293464   105.2   950   8.66     NP_001246234   AFH07989 Pfk-PE   FBpp0305281   102.5   922   8.58     NP_001260847   AGB93380
Additional Polypeptide Data & Comments
Reported size (kDa)	787 (aa) (Currie and Sullivan, 1994)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Phosphofructokinase, conserved site (IPR015912) Phosphofructokinase domain (IPR000023) 6-phosphofructokinase, eukaryotic type (IPR009161) Phosphofructokinase (IPR022953)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA540119   AA567976   AA697976   AA698608   AE013599 AAF58840   AE013599 AAF58841   AE013599 AAM71065   AF145673 AAD38648   AE013599 AFH07989   AE013599 AGB93380   AI062145   AI107433   AI107593   AI107668   AI134176   AI134667   AI257838   AI292508   AI387835   AI389665   AI402283   AI403616   AI514182   AI515180   AI515181   AI517261   AI517389   AQ025651   AY231725   BF485644   BF486589   BF489499   BF490735   BF492055   BF492640   BF492641   BF493252   BF493644   BF494666   BF498214   BF499574   BF500237   BF501578   BF501752   BF502700   BF503394   BF503579   BF503704   BF506747   BG633252   BG634094   BG635071   BI162011   BI162012   BI169176   BI173259   BI215498   BI241234   BI242641   BI364292   BI365878   BI373193   BI579323   BI580466   BI580515   BI605820   BI614093   BI614319   BI620397   BI631795   BP552019   BT001354 AAN71109   BT099630   BT099750   BT133189   CK605375   CK605376   CK659082   CK661392   CO284955   CO290948   CO292801   CO297011   CO297099   CO301732   CO309302   CO312540   CZ466945   CZ469245   CZ470428   CZ470732   EC051072   EC051593   EC053589   EC055577   EC056015   EC056438   EC056618   EC085539   EC089673   EC219286   EC222111   EC225454   EC225740   EC236985   EV577398   EV586135   GH941386   GH941962   GH944241   GH944644   GH948947   GH951653   GH952153   GH956193   GH961891   GH962280   GH962354   GH962387   GH962879   GH962908   GH963920   GH964097   GH966868   GH968338   GH969285   GH969825   GH971359   GH973016   GH973452   GH974530   GH977352   GH978599   GH978804   GH980215   GH980414   GH980692   GH981236   L27653 AAA62385
UniProtKB/Swiss-Prot	P52034
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 13 -- 16   dorsal pharyngeal muscle   (Fisher et al., 2012) embryonic/larval muscle system   (Fisher et al., 2012) embryonic/larval somatic muscle   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Currie and Sullivan, 1994)
Additional Descriptive Data
	Pfk transcripts are detected at a high level in early embryos, decline at mid embryogenesis, and increase to a plateau during larval stages. They decrease in abundance during pupation and increase just prior to emergence. mRNA levels rapidly decline after emergence even though Pfk enzyme activity remains high in adults. (Currie and Sullivan, 1994)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
enzyme assay or biochemical detection Stage Tissue/Position (including subcellular localization) Reference larval stage -- adult stage     (Munneke and Collier, 1985) mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Dorus et al., 2006, Wasbrough et al., 2010) day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
	Pfk specific activity was found to be very low during larval and early pupal stages. Activity plateaus at 24 hours posteclosion. (Munneke and Collier, 1985)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult hindgut, adult heart, adult fat body, adult salivary gland, adult spermathecae, adult testis, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult midgut, larval hindgut, larval/adult Malpighian tubules, larval fat body, larval salivary gland, larval trachea, adult ovary, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1055.475 Larval Midgut    140.9 Larval Hindgut    363.4 Larval Malpighian Tubules    181.1 Larval Fat Body    202.6 Larval Salivary Gland    390.9 Larval Trachea    228.775 Larval Carcass    2196.45 Adult Head    652.9 Adult Eye    681.525 Adult Brain    641.6 Adult Thoracic-Abdominal Ganglion    749.7 Adult Crop    811.9 Adult Midgut    173.3 Adult Hindgut    944.7 Adult Malpighian Tubules    404.3 Adult Fat Body    533.1 Adult Salivary Gland    842.4 Adult Heart    539.2 Adult VirginFemale Spermatheca    652.3 Adult InseminatedFemale Spermatheca    611 Adult Ovary    396.4 Adult Testis    925.9 Adult Male Accessory Gland    195.4 Adult Carcass    1120 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1055.475) Larval Midgut    140.9 Larval Hindgut    363.4 Larval Malpighian Tubules    181.1 Larval Fat Body    202.6 Larval Salivary Gland    390.9 Larval Trachea    228.775 Larval Carcass  (2196.45) Adult Head  (652.9) Adult Eye  (681.525) Adult Brain  (641.6) Adult Thoracic-Abdominal Ganglion  (749.7) Adult Crop  (811.9) Adult Midgut    173.3 Adult Hindgut  (944.7) Adult Malpighian Tubules    404.3 Adult Fat Body  533.1 Adult Salivary Gland  (842.4) Adult Heart  539.2 Adult VirginFemale Spermatheca  (652.3) Adult InseminatedFemale Spermatheca  (611) Adult Ovary    396.4 Adult Testis  (925.9) Adult Male Accessory Gland    195.4 Adult Carcass  (1120) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1055.475 Larval Midgut    140.9 Larval Hindgut    363.4 Larval Malpighian Tubules    181.1 Larval Fat Body    202.6 Larval Salivary Gland    390.9 Larval Trachea    228.775 Larval Carcass  (2196.45) Adult Head    652.9 Adult Eye    681.525 Adult Brain    641.6 Adult Thoracic-Abdominal Ganglion    749.7 Adult Crop    811.9 Adult Midgut    173.3 Adult Hindgut    944.7 Adult Malpighian Tubules    404.3 Adult Fat Body    533.1 Adult Salivary Gland    842.4 Adult Heart    539.2 Adult VirginFemale Spermatheca    652.3 Adult InseminatedFemale Spermatheca    611 Adult Ovary    396.4 Adult Testis    925.9 Adult Male Accessory Gland    195.4 Adult Carcass  (1120) Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1055.475 Larval Midgut    140.9 Larval Hindgut    363.4 Larval Malpighian Tubules    181.1 Larval Fat Body    202.6 Larval Salivary Gland    390.9 Larval Trachea    228.775 Larval Carcass    2196.45 Adult Head    652.9 Adult Eye    681.525 Adult Brain    641.6 Adult Thoracic-Abdominal Ganglion    749.7 Adult Crop    811.9 Adult Midgut    173.3 Adult Hindgut    944.7 Adult Malpighian Tubules    404.3 Adult Fat Body    533.1 Adult Salivary Gland    842.4 Adult Heart    539.2 Adult VirginFemale Spermatheca    652.3 Adult InseminatedFemale Spermatheca    611 Adult Ovary    396.4 Adult Testis    925.9 Adult Male Accessory Gland    195.4 Adult Carcass    1120 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1055.475 Larval Midgut    140.9 Larval Hindgut    363.4 Larval Malpighian Tubules    181.1 Larval Fat Body    202.6 Larval Salivary Gland    390.9 Larval Trachea    228.775 Larval Carcass    2196.45 Adult Head    652.9 Adult Eye    681.525 Adult Brain    641.6 Adult Thoracic-Abdominal Ganglion    749.7 Adult Crop    811.9 Adult Midgut    173.3 Adult Hindgut    944.7 Adult Malpighian Tubules    404.3 Adult Fat Body    533.1 Adult Salivary Gland    842.4 Adult Heart    539.2 Adult VirginFemale Spermatheca    652.3 Adult InseminatedFemale Spermatheca    611 Adult Ovary    396.4 Adult Testis    925.9 Adult Male Accessory Gland    195.4 Adult Carcass    1120 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1055.475) Larval Midgut    140.9 Larval Hindgut    363.4 Larval Malpighian Tubules    181.1 Larval Fat Body    202.6 Larval Salivary Gland    390.9 Larval Trachea    228.775 Larval Carcass  (2196.45) Adult Head  652.9 Adult Eye  681.525 Adult Brain  641.6 Adult Thoracic-Abdominal Ganglion  749.7 Adult Crop  811.9 Adult Midgut    173.3 Adult Hindgut  (944.7) Adult Malpighian Tubules    404.3 Adult Fat Body  533.1 Adult Salivary Gland  (842.4) Adult Heart  539.2 Adult VirginFemale Spermatheca  652.3 Adult InseminatedFemale Spermatheca  611 Adult Ovary    396.4 Adult Testis  (925.9) Adult Male Accessory Gland    195.4 Adult Carcass  (1120) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1055.475 Larval Midgut    140.9 Larval Hindgut    363.4 Larval Malpighian Tubules    181.1 Larval Fat Body    202.6 Larval Salivary Gland    390.9 Larval Trachea    228.775 Larval Carcass  (2196.45) Adult Head    652.9 Adult Eye    681.525 Adult Brain    641.6 Adult Thoracic-Abdominal Ganglion    749.7 Adult Crop    811.9 Adult Midgut    173.3 Adult Hindgut    944.7 Adult Malpighian Tubules    404.3 Adult Fat Body    533.1 Adult Salivary Gland    842.4 Adult Heart    539.2 Adult VirginFemale Spermatheca    652.3 Adult InseminatedFemale Spermatheca    611 Adult Ovary    396.4 Adult Testis    925.9 Adult Male Accessory Gland    195.4 Adult Carcass  1120 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1055.475 Larval Midgut    140.9 Larval Hindgut    363.4 Larval Malpighian Tubules    181.1 Larval Fat Body    202.6 Larval Salivary Gland    390.9 Larval Trachea    228.775 Larval Carcass    2196.45 Adult Head    652.9 Adult Eye    681.525 Adult Brain    641.6 Adult Thoracic-Abdominal Ganglion    749.7 Adult Crop    811.9 Adult Midgut    173.3 Adult Hindgut    944.7 Adult Malpighian Tubules    404.3 Adult Fat Body    533.1 Adult Salivary Gland    842.4 Adult Heart    539.2 Adult VirginFemale Spermatheca    652.3 Adult InseminatedFemale Spermatheca    611 Adult Ovary    396.4 Adult Testis    925.9 Adult Male Accessory Gland    195.4 Adult Carcass    1120 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    652.9   NA  Eye    681.525   NA  Brain    641.6   1055.475  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    749.7   NA  Crop    811.9   140.9  Midgut    173.3   363.4  Hindgut    944.7   181.1  Malpighian Tubules    404.3   202.6  Fat Body    533.1   390.9  Salivary Gland    842.4   NA  Heart    539.2   228.775  Trachea    NA   NA  VirginFemale Spermatheca    652.3   NA  InseminatedFemale Spermatheca    611   NA  Ovary    396.4   NA  Testis    925.9   NA  Male Accessory Gland    195.4   2196.45  Carcass    1120 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    49 central nervous system, larvae L3    19 central nervous system, pupae P8    10 head, virgin 1-day female    62 head, virgin 4-day female    38 head, virgin 20-day female    41 head, mated 1-day female    44 head, mated 4-day female    34 head, mated 20-day female    31 head, mated 1-day male    72 head, mated 4-day male    56 head, mated 20-day male    59 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    14 digestive system, 4-day adult    14 digestive system, 20-day adult    15 fat body, larvae L3 wandering    6 fat body, white prepupae    19 fat body, pupae P8    13 carcass, larvae L3 wandering    29 carcass, 1-day adult    110 carcass, 4-day adult    48 carcass, 20-day adult    29 ovary, virgin 4-day female    31 ovary, mated 4-day female    24 testis, mated 4-day male    100 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (49) central nervous system, larvae L3    19 central nervous system, pupae P8    10 head, virgin 1-day female  (62) head, virgin 4-day female  (38) head, virgin 20-day female  (41) head, mated 1-day female  (44) head, mated 4-day female  (34) head, mated 20-day female  (31) head, mated 1-day male  (72) head, mated 4-day male  (56) head, mated 20-day male  (59) salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    14 digestive system, 4-day adult    14 digestive system, 20-day adult    15 fat body, larvae L3 wandering    6 fat body, white prepupae    19 fat body, pupae P8    13 carcass, larvae L3 wandering  (29) carcass, 1-day adult  (110) carcass, 4-day adult  (48) carcass, 20-day adult  (29) ovary, virgin 4-day female  (31) ovary, mated 4-day female    24 testis, mated 4-day male  (100) accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    49 central nervous system, larvae L3    19 central nervous system, pupae P8    10 head, virgin 1-day female    62 head, virgin 4-day female    38 head, virgin 20-day female    41 head, mated 1-day female    44 head, mated 4-day female    34 head, mated 20-day female    31 head, mated 1-day male    72 head, mated 4-day male    56 head, mated 20-day male    59 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    14 digestive system, 4-day adult    14 digestive system, 20-day adult    15 fat body, larvae L3 wandering    6 fat body, white prepupae    19 fat body, pupae P8    13 carcass, larvae L3 wandering    29 carcass, 1-day adult  (110) carcass, 4-day adult    48 carcass, 20-day adult    29 ovary, virgin 4-day female    31 ovary, mated 4-day female    24 testis, mated 4-day male    100 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    49 central nervous system, larvae L3    19 central nervous system, pupae P8    10 head, virgin 1-day female    62 head, virgin 4-day female    38 head, virgin 20-day female    41 head, mated 1-day female    44 head, mated 4-day female    34 head, mated 20-day female    31 head, mated 1-day male    72 head, mated 4-day male    56 head, mated 20-day male    59 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    14 digestive system, 4-day adult    14 digestive system, 20-day adult    15 fat body, larvae L3 wandering    6 fat body, white prepupae    19 fat body, pupae P8    13 carcass, larvae L3 wandering    29 carcass, 1-day adult    110 carcass, 4-day adult    48 carcass, 20-day adult    29 ovary, virgin 4-day female    31 ovary, mated 4-day female    24 testis, mated 4-day male    100 accessory gland, mated 4-day male    16 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    49 central nervous system, larvae L3    19 central nervous system, pupae P8    10 head, virgin 1-day female    62 head, virgin 4-day female    38 head, virgin 20-day female    41 head, mated 1-day female    44 head, mated 4-day female    34 head, mated 20-day female    31 head, mated 1-day male    72 head, mated 4-day male    56 head, mated 20-day male    59 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    14 digestive system, 4-day adult    14 digestive system, 20-day adult    15 fat body, larvae L3 wandering    6 fat body, white prepupae    19 fat body, pupae P8    13 carcass, larvae L3 wandering    29 carcass, 1-day adult    110 carcass, 4-day adult    48 carcass, 20-day adult    29 ovary, virgin 4-day female    31 ovary, mated 4-day female    24 testis, mated 4-day male    100 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (49) central nervous system, larvae L3    19 central nervous system, pupae P8    10 head, virgin 1-day female  (62) head, virgin 4-day female  (38) head, virgin 20-day female  (41) head, mated 1-day female  (44) head, mated 4-day female  (34) head, mated 20-day female  31 head, mated 1-day male  (72) head, mated 4-day male  (56) head, mated 20-day male  (59) salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    14 digestive system, 4-day adult    14 digestive system, 20-day adult    15 fat body, larvae L3 wandering    6 fat body, white prepupae    19 fat body, pupae P8    13 carcass, larvae L3 wandering  29 carcass, 1-day adult  (110) carcass, 4-day adult  (48) carcass, 20-day adult  29 ovary, virgin 4-day female  31 ovary, mated 4-day female    24 testis, mated 4-day male  (100) accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    49 central nervous system, larvae L3    19 central nervous system, pupae P8    10 head, virgin 1-day female    62 head, virgin 4-day female    38 head, virgin 20-day female    41 head, mated 1-day female    44 head, mated 4-day female    34 head, mated 20-day female    31 head, mated 1-day male    72 head, mated 4-day male    56 head, mated 20-day male    59 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    14 digestive system, 4-day adult    14 digestive system, 20-day adult    15 fat body, larvae L3 wandering    6 fat body, white prepupae    19 fat body, pupae P8    13 carcass, larvae L3 wandering    29 carcass, 1-day adult  110 carcass, 4-day adult    48 carcass, 20-day adult    29 ovary, virgin 4-day female    31 ovary, mated 4-day female    24 testis, mated 4-day male    100 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    49 central nervous system, larvae L3    19 central nervous system, pupae P8    10 head, virgin 1-day female    62 head, virgin 4-day female    38 head, virgin 20-day female    41 head, mated 1-day female    44 head, mated 4-day female    34 head, mated 20-day female    31 head, mated 1-day male    72 head, mated 4-day male    56 head, mated 20-day male    59 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    14 digestive system, 4-day adult    14 digestive system, 20-day adult    15 fat body, larvae L3 wandering    6 fat body, white prepupae    19 fat body, pupae P8    13 carcass, larvae L3 wandering    29 carcass, 1-day adult    110 carcass, 4-day adult    48 carcass, 20-day adult    29 ovary, virgin 4-day female    31 ovary, mated 4-day female    24 testis, mated 4-day male    100 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed during early larval stages, during late pupal stages, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    51 embryo 02-04hr    15 embryo 04-06hr    3 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    12 embryo 12-14hr    17 embryo 14-16hr    46 embryo 16-18hr    61 embryo 18-20hr    86 embryo 20-22hr    111 embryo 22-24hr    107 larva L1    131 larva L2    113 larva L3 12hr old    122 larva L3 puffstage 1-2    82 larva L3 puffstage 3-6    57 larva L3 puffstage 7-9    29 white prepupae new    30 white prepupae 12hr    39 white prepupae 24hr    38 pupae 2d postWPP    37 pupae 3d postWPP    113 pupae 4d postWPP    280 adult male 01day    197 adult male 05day    158 adult male 30day    123 adult female 01day    144 adult female 05day    52 adult female 30day    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (51) embryo 02-04hr    15 embryo 04-06hr    3 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    12 embryo 12-14hr    17 embryo 14-16hr  (46) embryo 16-18hr  (61) embryo 18-20hr  (86) embryo 20-22hr  (111) embryo 22-24hr  (107) larva L1  (131) larva L2  (113) larva L3 12hr old  (122) larva L3 puffstage 1-2  (82) larva L3 puffstage 3-6  (57) larva L3 puffstage 7-9  (29) white prepupae new  (30) white prepupae 12hr  (39) white prepupae 24hr  (38) pupae 2d postWPP  (37) pupae 3d postWPP  (113) pupae 4d postWPP  (280) adult male 01day  (197) adult male 05day  (158) adult male 30day  (123) adult female 01day  (144) adult female 05day  (52) adult female 30day  (41) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    51 embryo 02-04hr    15 embryo 04-06hr    3 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    12 embryo 12-14hr    17 embryo 14-16hr    46 embryo 16-18hr    61 embryo 18-20hr    86 embryo 20-22hr  (111) embryo 22-24hr  107 larva L1  (131) larva L2  (113) larva L3 12hr old  (122) larva L3 puffstage 1-2    82 larva L3 puffstage 3-6    57 larva L3 puffstage 7-9    29 white prepupae new    30 white prepupae 12hr    39 white prepupae 24hr    38 pupae 2d postWPP    37 pupae 3d postWPP  (113) pupae 4d postWPP  (280) adult male 01day  (197) adult male 05day  (158) adult male 30day  (123) adult female 01day  (144) adult female 05day    52 adult female 30day    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    51 embryo 02-04hr    15 embryo 04-06hr    3 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    12 embryo 12-14hr    17 embryo 14-16hr    46 embryo 16-18hr    61 embryo 18-20hr    86 embryo 20-22hr    111 embryo 22-24hr    107 larva L1    131 larva L2    113 larva L3 12hr old    122 larva L3 puffstage 1-2    82 larva L3 puffstage 3-6    57 larva L3 puffstage 7-9    29 white prepupae new    30 white prepupae 12hr    39 white prepupae 24hr    38 pupae 2d postWPP    37 pupae 3d postWPP    113 pupae 4d postWPP    280 adult male 01day    197 adult male 05day    158 adult male 30day    123 adult female 01day    144 adult female 05day    52 adult female 30day    41 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    51 embryo 02-04hr    15 embryo 04-06hr    3 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    12 embryo 12-14hr    17 embryo 14-16hr    46 embryo 16-18hr    61 embryo 18-20hr    86 embryo 20-22hr    111 embryo 22-24hr    107 larva L1    131 larva L2    113 larva L3 12hr old    122 larva L3 puffstage 1-2    82 larva L3 puffstage 3-6    57 larva L3 puffstage 7-9    29 white prepupae new    30 white prepupae 12hr    39 white prepupae 24hr    38 pupae 2d postWPP    37 pupae 3d postWPP    113 pupae 4d postWPP    280 adult male 01day    197 adult male 05day    158 adult male 30day    123 adult female 01day    144 adult female 05day    52 adult female 30day    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (51) embryo 02-04hr    15 embryo 04-06hr    3 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    12 embryo 12-14hr    17 embryo 14-16hr  (46) embryo 16-18hr  (61) embryo 18-20hr  (86) embryo 20-22hr  (111) embryo 22-24hr  (107) larva L1  (131) larva L2  (113) larva L3 12hr old  (122) larva L3 puffstage 1-2  (82) larva L3 puffstage 3-6  (57) larva L3 puffstage 7-9  29 white prepupae new  30 white prepupae 12hr  (39) white prepupae 24hr  (38) pupae 2d postWPP  (37) pupae 3d postWPP  (113) pupae 4d postWPP  (280) adult male 01day  (197) adult male 05day  (158) adult male 30day  (123) adult female 01day  (144) adult female 05day  (52) adult female 30day  (41) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    51 embryo 02-04hr    15 embryo 04-06hr    3 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    12 embryo 12-14hr    17 embryo 14-16hr    46 embryo 16-18hr    61 embryo 18-20hr    86 embryo 20-22hr  111 embryo 22-24hr  107 larva L1  131 larva L2  113 larva L3 12hr old  122 larva L3 puffstage 1-2    82 larva L3 puffstage 3-6    57 larva L3 puffstage 7-9    29 white prepupae new    30 white prepupae 12hr    39 white prepupae 24hr    38 pupae 2d postWPP    37 pupae 3d postWPP  113 pupae 4d postWPP  (280) adult male 01day  (197) adult male 05day  (158) adult male 30day  123 adult female 01day  (144) adult female 05day    52 adult female 30day    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    51 embryo 02-04hr    15 embryo 04-06hr    3 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    12 embryo 12-14hr    17 embryo 14-16hr    46 embryo 16-18hr    61 embryo 18-20hr    86 embryo 20-22hr    111 embryo 22-24hr    107 larva L1    131 larva L2    113 larva L3 12hr old    122 larva L3 puffstage 1-2    82 larva L3 puffstage 3-6    57 larva L3 puffstage 7-9    29 white prepupae new    30 white prepupae 12hr    39 white prepupae 24hr    38 pupae 2d postWPP    37 pupae 3d postWPP    113 pupae 4d postWPP    280 adult male 01day    197 adult male 05day    158 adult male 30day    123 adult female 01day    144 adult female 05day    52 adult female 30day    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    16 embryonic 1182-4H    19 embryonic GM2    17 embryonic Kc167    64 embryonic S1    16 embryonic S3    9 leg disc CME L1    25 wing disc CME-W2    47 wing disc ML-DmD8    51 wing disc ML-DmD9    49 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    20 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    84 CNS ML-DmBG2-c2    142 tumorous blood cells mbn2    41 ovary fGS/OSS    26 ovary OSC    22 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  26 Schneider line 2 Sg4    16 embryonic 1182-4H    19 embryonic GM2    17 embryonic Kc167  (64) embryonic S1    16 embryonic S3    9 leg disc CME L1    25 wing disc CME-W2  (47) wing disc ML-DmD8  (51) wing disc ML-DmD9  (49) wing disc ML-DmD16-c3    23 wing disc ML-DmD21    20 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1  (34) CNS ML-DmBG1-c1  (84) CNS ML-DmBG2-c2  (142) tumorous blood cells mbn2  (41) ovary fGS/OSS  26 ovary OSC    22 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    16 embryonic 1182-4H    19 embryonic GM2    17 embryonic Kc167    64 embryonic S1    16 embryonic S3    9 leg disc CME L1    25 wing disc CME-W2    47 wing disc ML-DmD8    51 wing disc ML-DmD9    49 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    20 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    84 CNS ML-DmBG2-c2  (142) tumorous blood cells mbn2    41 ovary fGS/OSS    26 ovary OSC    22 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    16 embryonic 1182-4H    19 embryonic GM2    17 embryonic Kc167    64 embryonic S1    16 embryonic S3    9 leg disc CME L1    25 wing disc CME-W2    47 wing disc ML-DmD8    51 wing disc ML-DmD9    49 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    20 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    84 CNS ML-DmBG2-c2    142 tumorous blood cells mbn2    41 ovary fGS/OSS    26 ovary OSC    22 ovary OSS    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    16 embryonic 1182-4H    19 embryonic GM2    17 embryonic Kc167    64 embryonic S1    16 embryonic S3    9 leg disc CME L1    25 wing disc CME-W2    47 wing disc ML-DmD8    51 wing disc ML-DmD9    49 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    20 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    84 CNS ML-DmBG2-c2    142 tumorous blood cells mbn2    41 ovary fGS/OSS    26 ovary OSC    22 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  26 Schneider line 2 Sg4    16 embryonic 1182-4H    19 embryonic GM2    17 embryonic Kc167  (64) embryonic S1    16 embryonic S3    9 leg disc CME L1    25 wing disc CME-W2  (47) wing disc ML-DmD8  (51) wing disc ML-DmD9  (49) wing disc ML-DmD16-c3    23 wing disc ML-DmD21    20 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1  (34) CNS ML-DmBG1-c1  (84) CNS ML-DmBG2-c2  (142) tumorous blood cells mbn2  (41) ovary fGS/OSS  26 ovary OSC    22 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    16 embryonic 1182-4H    19 embryonic GM2    17 embryonic Kc167    64 embryonic S1    16 embryonic S3    9 leg disc CME L1    25 wing disc CME-W2    47 wing disc ML-DmD8    51 wing disc ML-DmD9    49 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    20 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    84 CNS ML-DmBG2-c2  142 tumorous blood cells mbn2    41 ovary fGS/OSS    26 ovary OSC    22 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    16 embryonic 1182-4H    19 embryonic GM2    17 embryonic Kc167    64 embryonic S1    16 embryonic S3    9 leg disc CME L1    25 wing disc CME-W2    47 wing disc ML-DmD8    51 wing disc ML-DmD9    49 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    20 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11    19 antennal disc ML-DmD20-c5    10 mixed discs ML-DmD4-c1    34 CNS ML-DmBG1-c1    84 CNS ML-DmBG2-c2    142 tumorous blood cells mbn2    41 ovary fGS/OSS    26 ovary OSC    22 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    14 heat shock, 4-day adult    74 Cadmium 50 mM 6 hrs, larvae L3    80 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    88 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    61 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    35 Zinc 4.5 mM 48 hrs, 4-day adult    25 Ethanol 2.5% 3 hrs, larvae L3    61 Ethanol 5% 3 hrs, larvae L3    75 Ethanol 10% 3 hrs, larvae L3    89 Caffeine 1.5 mg/ml 4 hrs, larvae L3    70 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    50 Paraquat 10 mM 48 hrs, 4-day adult    101 Rotenone 2 μg 12 hrs, larvae L3    62 Rotenone 8 μg 12 hrs, larvae L3    121 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    14 heat shock, 4-day adult  (74) Cadmium 50 mM 6 hrs, larvae L3  (80) Cadmium 50 mM 12 hrs, larvae L3  (35) Cadmium 50 mM 48 hrs, 4-day adult  (88) Cadmium 100 mM 48 hrs, 4-day adult  (37) Copper 0.5 mM 12 hrs, larvae L3  (61) Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3  (35) Zinc 4.5 mM 48 hrs, 4-day adult    25 Ethanol 2.5% 3 hrs, larvae L3  (61) Ethanol 5% 3 hrs, larvae L3  (75) Ethanol 10% 3 hrs, larvae L3  (89) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (70) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  26 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult  (50) Paraquat 10 mM 48 hrs, 4-day adult  (101) Rotenone 2 μg 12 hrs, larvae L3  (62) Rotenone 8 μg 12 hrs, larvae L3  (121) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    14 heat shock, 4-day adult    74 Cadmium 50 mM 6 hrs, larvae L3    80 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    88 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    61 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    35 Zinc 4.5 mM 48 hrs, 4-day adult    25 Ethanol 2.5% 3 hrs, larvae L3    61 Ethanol 5% 3 hrs, larvae L3    75 Ethanol 10% 3 hrs, larvae L3    89 Caffeine 1.5 mg/ml 4 hrs, larvae L3    70 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    50 Paraquat 10 mM 48 hrs, 4-day adult  101 Rotenone 2 μg 12 hrs, larvae L3    62 Rotenone 8 μg 12 hrs, larvae L3  (121) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    14 heat shock, 4-day adult    74 Cadmium 50 mM 6 hrs, larvae L3    80 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    88 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    61 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    35 Zinc 4.5 mM 48 hrs, 4-day adult    25 Ethanol 2.5% 3 hrs, larvae L3    61 Ethanol 5% 3 hrs, larvae L3    75 Ethanol 10% 3 hrs, larvae L3    89 Caffeine 1.5 mg/ml 4 hrs, larvae L3    70 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    50 Paraquat 10 mM 48 hrs, 4-day adult    101 Rotenone 2 μg 12 hrs, larvae L3    62 Rotenone 8 μg 12 hrs, larvae L3    121 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    14 heat shock, 4-day adult    74 Cadmium 50 mM 6 hrs, larvae L3    80 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    88 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    61 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    35 Zinc 4.5 mM 48 hrs, 4-day adult    25 Ethanol 2.5% 3 hrs, larvae L3    61 Ethanol 5% 3 hrs, larvae L3    75 Ethanol 10% 3 hrs, larvae L3    89 Caffeine 1.5 mg/ml 4 hrs, larvae L3    70 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    50 Paraquat 10 mM 48 hrs, 4-day adult    101 Rotenone 2 μg 12 hrs, larvae L3    62 Rotenone 8 μg 12 hrs, larvae L3    121 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    14 heat shock, 4-day adult  (74) Cadmium 50 mM 6 hrs, larvae L3  (80) Cadmium 50 mM 12 hrs, larvae L3  (35) Cadmium 50 mM 48 hrs, 4-day adult  (88) Cadmium 100 mM 48 hrs, 4-day adult  (37) Copper 0.5 mM 12 hrs, larvae L3  (61) Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3  (35) Zinc 4.5 mM 48 hrs, 4-day adult    25 Ethanol 2.5% 3 hrs, larvae L3  (61) Ethanol 5% 3 hrs, larvae L3  (75) Ethanol 10% 3 hrs, larvae L3  (89) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (70) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  26 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult  (50) Paraquat 10 mM 48 hrs, 4-day adult  (101) Rotenone 2 μg 12 hrs, larvae L3  (62) Rotenone 8 μg 12 hrs, larvae L3  (121) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    14 heat shock, 4-day adult    74 Cadmium 50 mM 6 hrs, larvae L3    80 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    88 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    61 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    35 Zinc 4.5 mM 48 hrs, 4-day adult    25 Ethanol 2.5% 3 hrs, larvae L3    61 Ethanol 5% 3 hrs, larvae L3    75 Ethanol 10% 3 hrs, larvae L3    89 Caffeine 1.5 mg/ml 4 hrs, larvae L3    70 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    50 Paraquat 10 mM 48 hrs, 4-day adult  101 Rotenone 2 μg 12 hrs, larvae L3    62 Rotenone 8 μg 12 hrs, larvae L3  121 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    14 heat shock, 4-day adult    74 Cadmium 50 mM 6 hrs, larvae L3    80 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    88 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    61 Copper 15 mM 48 hrs, 4-day adult    20 Zinc 5 mM 12 hrs, larvae L3    35 Zinc 4.5 mM 48 hrs, 4-day adult    25 Ethanol 2.5% 3 hrs, larvae L3    61 Ethanol 5% 3 hrs, larvae L3    75 Ethanol 10% 3 hrs, larvae L3    89 Caffeine 1.5 mg/ml 4 hrs, larvae L3    70 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    50 Paraquat 10 mM 48 hrs, 4-day adult    101 Rotenone 2 μg 12 hrs, larvae L3    62 Rotenone 8 μg 12 hrs, larvae L3    121 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_10 mE1_20_mRNA_expression_cluster_15
External Data & Images
Linkouts	BDGP expression data • GH12192 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003071 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4001-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Pfk Stages(s) 1-3
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4Act5C.PU PfkGD1508 lethal (with Df(2R)X1) Pfk06339 lethal | recessive Pfk06339 viable, with Scer\GAL4pnr-MD237 PfkGD1508 Phenotype manifest in Allele
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of Pfk Class Mutagen Stocks Known lesion Pfk06339 P-element activity 1 -- Pfkc01107 1 -- Pfkc01155 piggyBac transposase 1 -- Pfkc06016 piggyBac transposase 1 -- Pfkc06496 piggyBac transposase 1 -- PfkEY23126 P-element activity 1 -- Pfkc04430 piggyBac transposase 0 -- Pfkin5 natural population 0 Yes
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Pfk Class Mutagen Stocks Known lesion PfkGD1508 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes PfkGL00298 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes PfkHMS01324 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes PfkKK101887 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes PfkScer\UAS.cTa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)X1 (Spradling et al., 1999, Goldstein et al., 2001, BDGP Project Members, 1994-1999)
Not disrupted in	Df(2R)12 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)en-A (BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1508} NA P{KK101887} NA P{TRiP.GL00298} NA P{TRiP.HMS01324} NA P{UAS-Pfk.T} NA reporter construct P{Pfk-lacZ.T} No
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{PZ}Pfk06339 No miscellaneous insertions PBac{PB}Pfkc01107 NA PBac{PB}Pfkc01155 NA PBac{PB}Pfkc04430 NA PBac{PB}Pfkc06016 NA PBac{PB}Pfkc06496 NA roo{}Pfkin5 NA insertion of mobile activating element P{EPgy2}PfkEY23126 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function
CV term Evidence References
6-phosphofructokinase activity inferred from mutant phenotype (Currie and Sullivan, 1994)
Biological Process
CV term Evidence References
glycolysis inferred from mutant phenotype (Currie and Sullivan, 1994)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
6-phosphofructokinase activity inferred from sequence or structural similarity with UniProtKB:Q27778 (FlyBase, 1992-)
Biological Process
CV term Evidence References
fructose 6-phosphate metabolic process inferred from electronic annotation with InterPro:IPR022953 (FlyBase Curators et al., 2004-) glycolysis non-traceable author statement (Swiss-Prot Project Members, 2001.8.20)
Cellular Component
CV term Evidence References
6-phosphofructokinase complex inferred by curator from GO:0003872 (Currie and Sullivan, 1994) inferred from electronic annotation with InterPro:IPR000023, InterPro:IPR015912 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Pfk - CG12237 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pfk - CG14507 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pfk - CG1492 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pfk - CG3570 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pfk - CG5027 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pfk - CG5190 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pfk - CG6330 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pfk - CG8005 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pfk - Stat92E anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pfk - TfIIEα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Pfk allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61888 DroID - A comprehensive database of gene and protein interactions. • FBgn0003071 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36060
Orthologs
OrthoDB Orthologs (52) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG63BN7B Dipteran inclusive ortholog search EOG6CG0H9 Insect inclusive ortholog search EOG69KDKF Arthropod inclusive ortholog search EOG6K0P38 Metazoa inclusive ortholog search EOG67X1FH
Orthologs in Drosophila Species (EOG63BN7B)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Pfk     Drosophila simulans   Dsim\GD10715 Y   Drosophila sechellia   Dsec\GM21187 Y   Drosophila erecta   Dere\GG24141 Y   Drosophila yakuba   Dyak\Pfk Y   Drosophila ananassae   Dana\GF12020 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA17840 Y   Drosophila persimilis   Dper\GL17610 Y   Drosophila willistoni   Dwil\GK21487 Y   Drosophila virilis   Dvir\GJ22388 Y   Drosophila mojavensis   Dmoj\GI20539 Y   Drosophila grimshawi   Dgri\GH21789 Y
Orthologs in non-Drosophila Dipterans (EOG6CG0H9)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL006895   Anopheles gambiae Malaria mosquito  Agam\AGAP007642   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ008591
Orthologs in non-Dipteran Insects (EOG69KDKF)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB50943   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG004643   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH14127   Atta cephalotes Leafcutter ant  Acep\ACEP27229   Camponotus floridanus Florida carpenter ant  Cflo\CFLO22828   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL18122   Linepithema humile Argentine ant  Lhum\LH15038   Pogonomyrmex barbatus Red harvester ant  Pbar\PB17626   Acyrthosiphon pisum Pea aphid  Apim\ACYPI001940   Acyrthosiphon pisum Pea aphid  Apim\ACYPI001033   Bombyx mori Silkmoth  Bmor\BGIBMGA003717   Pediculus humanus Human body louse  Phum\PHUM615880   Tribolium castaneum Red flour beetle  Tcas\TC005855
Orthologs in non-Insect Arthropods (EOG6K0P38)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\GPR176   Ixodes scapularis Deer tick  Isca\ISCW014412
Orthologs in non-Arthropod Metazoa (EOG67X1FH)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\C50F4.2   Caenorhabditis elegans Nematode  Cele\Y71H10A.1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001831gn   Danio rerio Zebrafish  Drer\pfkma   Danio rerio Zebrafish  Drer\si:ch73-73n3.1   Danio rerio Zebrafish  Drer\pfkmb   Danio rerio Zebrafish  Drer\ENSDARG00000060504   Danio rerio Zebrafish  Drer\si:dkeyp-12a9.4   Xenopus tropicalis Western clawed frog  Xtro\pfkm   Xenopus tropicalis Western clawed frog  Xtro\pfkp   Gallus gallus Domestic chicken  Ggal\PFKP   Gallus gallus Domestic chicken  Ggal\PFKL   Gallus gallus Domestic chicken  Ggal\PFKM   Mus musculus House mouse  Mmus\Pfkp   Mus musculus House mouse  Mmus\Pfkl   Mus musculus House mouse  Mmus\Pfkm   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000017163   Rattus norvegicus Norway rat  Rnor\Pfkl   Rattus norvegicus Norway rat  Rnor\Pfkm   Homo sapiens Human  Hsap\ENSG00000152556   Homo sapiens Human  Hsap\PFKL   Homo sapiens Human  Hsap\PFKP
Human Orthologs (3)
Gene OMIM HGNC Hsap\ENSG00000152556     Hsap\PFKL     Hsap\PFKP
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10715 Drosophila sechellia Dsec\GM21187 Drosophila erecta Dere\GG24141 Drosophila yakuba Dyak\Pfk Drosophila ananassae Dana\GF12020 Drosophila pseudoobscura pseudoobscura Dpse\GA17840 Drosophila persimilis Dper\GL17610 Drosophila willistoni Dwil\GK21487 Drosophila virilis Dvir\GJ22388 Drosophila mojavensis Dmoj\GI20539 Drosophila grimshawi Dgri\GH21789
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	12031 cn1 P{PZ}Pfk06339/CyO; ry506 36782 y1 sc* v1; P{TRiP.GL00298}attP2 34336 y1 sc* v1; P{TRiP.HMS01324}attP2 22605 y1 w67c23; P{EPgy2}PfkEY23126/CyO
Harvard	- PBac{PB}Pfkc01107 - PBac{PB}Pfkc01155 - PBac{PB}Pfkc06016 - PBac{PB}Pfkc06496
VDRC	v105666 P{KK101887}VIE-260B v3016 w1118; P{GD1508}v3016 v3017 w1118; P{GD1508}v3017
Genomic Clones ( 2 )
	BACR06J01 BACR09J12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 120 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT27295 GH12192 LP08893 RE16470
Other clones	FI18643
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT01567 AT01960 AT03392 AT04787 AT04880 AT09311 AT12588 AT14162 AT15004 AT16949 AT17290 AT18507 AT19368 AT19741 AT20985 AT25457 AT28926 AT30154 AT31536 GH01235 GH05115 GH05352 GH05477 GH11557 GH15430 GH18504 GH20896 GH27255 GH27945 GH28317 HL02070 HL03554 HL05327 LD19495 LD46893 LD46894 LP06325 LP16555 LP20866 RE10272 RE16045 RE22014 RE38259 RE40037 RE49372 RE51272 RE60429 RE73340 RE74662 RE74721 RH43386 RH43667 RH51540 RH61443 RH71805
Other clones	38514 56239 104402 104552 108102 166225 AT01111 CK01.97D.G11 Dmel_dig_000962_0598_0368 Dmel_dig_016300_0655_3540 EC051072 EC051593 EC053589 EC055577 EC056015 EC056438 EC056618 EK075628 EK167718 EK182005 EK200964 EK215733 EK216003 EK217312 EK229228 FGM216B07 GH23092 RE01442 RP004083562 RP004148020 RP004154955 RP004189554 RP004233448 RP004238075 RP004238625 RP004241119 RP004242766 RP004242770 RP004255741 RP004258698 RP004260777 RP004268041 RP004294460 RP004361606 RP004381334 RP004388659 RP004414068 RP004436539 RP004443390 RP004456174 RP004474697 RP004507476 RP004509890 RP004519829 RP004520556 RP004525633 RP004525924 RP004527422 RP004530877 UT01881
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003071 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36060
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Pfk CG4001 (Shue, 1999.11)
Source for database merge of	Source for merge of: Pfk BcDNA:GH12192 (FlyBase, 1996-) Source for merge of: Pfk l(2)06339 (Spradling et al., 1999)
Additional comments	FlyBase curator comment: The "l(2)46Eb" complementation group is stated to correspond to Pfk in FBrf0145131, since members of this complementation group fail to complement the Pfk06339 insertion allele (the insertion in the Pfk06339 allele is within Pfk and multiple precise excisions can be obtained from this line, suggesting that the P-element insertion is responsible for the lethality of the line). However, this contradicts data from FBrf0076706 which suggests instead that the "l(2)46Fb" complementation group corresponds to Pfk. "l(2)46Fb", "l(2)46Eb" and Pfk have been kept as 3 separate genes in FlyBase until this discrepancy is resolved. (Goldstein et al., 2001) Source for merge of Pfk BcDNA:GH12192 was sequence comparison (date:990717). (FlyBase, 1996-) FlyBase curator comment: The "l(2)46Fb" complementation group is stated to correspond to Pfk in FBrf0076706, since animals which are heterozygous for "l(2)46Fb" mutations show an approximately 50% reduction in Pfk activity compared to wild-type controls. However, this contradicts data from FBrf0145131, which suggests instead that the "l(2)46Eb" complementation group corresponds to Pfk. "l(2)46Fb", "l(2)46Eb" and Pfk have been kept as 3 separate genes in FlyBase until this discrepancy is resolved. (Currie and Sullivan, 1994)
Other Comments
	Pfk is a single copy gene with a coding region that spans 6.5kb, composed of 8 exons and 7 intron. Alternate polyadenylation sites generate 3 developmentally regulated transcripts. Transcription initiation occurs at a single site. (Currie and Sullivan, 1994) Produces the glycolytic enzyme phosphofructokinase (PFK). Only one electrophoretic form has been found in larvae and adults.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078952 NM_165746 NM_165747 NM_001259305 NM_001273918 RefSeq (Proteins) • NP_523676 NP_724890 NP_724891 NP_001246234 NP_001260847 DDBJ / EMBL / GenBank • AA697976 AAA62385 AAD38648 AAF58840 AAF58841 AAM71065 AAN71109 AF145673 AFH07989 AGB93380 AI134667 AI402283 AQ025651 BF490735 BI162011 BI162012 BT001354 CZ466945 CZ469245 CZ470428 CZ470732 L27653 Entrez Gene - A searchable database of RefSeq genes. • 36060 UniProtKB/Swiss-Prot • P52034
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Phosphofructokinase, conserved site (IPR015912) Phosphofructokinase domain (IPR000023) 6-phosphofructokinase, eukaryotic type (IPR009161) Phosphofructokinase (IPR022953) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH12192
Linkouts
	BioGRID - A database of protein and genetic interactions • 61888 DroID - A comprehensive database of gene and protein interactions. • FBgn0003071 DRSC - Results from RNAi screens. • FBgn0003071 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003071 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4001-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003071 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36060 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36060 modMine - Data generated by the modENCODE project. • FBgn0003071
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	BcDNA:GH12192   BcDNA.GH12192 (Rubin, 1999.4.23) CG4001 (Pile et al., 2003, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Ni et al., 2010.12.1, Cammarato et al., 2011, Derré et al., 2007) l(2)06339 (Meister and Braun, 1995.10, BDGP Project Members, 1994-1999, ) Pfk (Rehwinkel et al., 2006, Dietzl et al., 2007, Gan et al., 2010, Dorus et al., 2006, Behm-Ansmant et al., 2006, Tennessen et al., 2011, Wasbrough et al., 2010, Derré et al., 2007) pfk (Currie, 1994) PFK (Currie and Sullivan, 1994)
Name Synonym	6-phosphofructokinase (Li and White, 2003) Phosphofructokinase (Barnes et al., 2010, Tennessen et al., 2011, Cammarato et al., 2011)
Secondary FlyBase IDs
	FBgn0010618
References ( 48 )
Generate a list of	All references relating to this gene ( 48 )
List References by type	Research paper  ( 17 ) Supplementary material  ( 8 ) Personal communication to FlyBase  ( 12 ) Abstract  ( 1 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 2 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 ) Website  ( 1 ) Book  ( 1 )
Recent research papers ( 2 )
	Li et al., 2013, PLoS Genet. 9(1): e1003230 HIF- and Non-HIF-Regulated Hypoxic Responses Require the Estrogen-Related Receptor in Drosophila melanogaster. [FBrf0220675] Tennessen et al., 2011, Cell Metab. 13(2): 139--148 The Drosophila Estrogen-Related Receptor Directs a Metabolic Switch that Supports Developmental Growth. [FBrf0212927] Show all research papers ...