Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.46
3.25, 3.0, 2.85 (northern blot)
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pfk using the Feature Mapper tool.
Pfk transcripts are detected at a high level in early embryos, decline at mid embryogenesis, and increase to a plateau during larval stages. They decrease in abundance during pupation and increase just prior to emergence. mRNA levels rapidly decline after emergence even though Pfk enzyme activity remains high in adults.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Pfk in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Pfk CG4001
FlyBase curator comment: The "l(2)46Eb" complementation group is stated to correspond to Pfk in FBrf0145131, since members of this complementation group fail to complement the Pfk06339 insertion allele (the insertion in the Pfk06339 allele is within Pfk and multiple precise excisions can be obtained from this line, suggesting that the P-element insertion is responsible for the lethality of the line). However, this contradicts data from FBrf0076706 which suggests instead that the "l(2)46Fb" complementation group corresponds to Pfk. "l(2)46Fb", "l(2)46Eb" and Pfk have been kept as 3 separate genes in FlyBase until this discrepancy is resolved.
Source for merge of Pfk BcDNA:GH12192 was sequence comparison ( date:990717 ).
FlyBase curator comment: The "l(2)46Fb" complementation group is stated to correspond to Pfk in FBrf0076706, since animals which are heterozygous for "l(2)46Fb" mutations show an approximately 50% reduction in Pfk activity compared to wild-type controls. However, this contradicts data from FBrf0145131, which suggests instead that the "l(2)46Eb" complementation group corresponds to Pfk. "l(2)46Fb", "l(2)46Eb" and Pfk have been kept as 3 separate genes in FlyBase until this discrepancy is resolved.
Pfk is a single copy gene with a coding region that spans 6.5kb, composed of 8 exons and 7 intron. Alternate polyadenylation sites generate 3 developmentally regulated transcripts. Transcription initiation occurs at a single site.
Produces the glycolytic enzyme phosphofructokinase (PFK). Only one electrophoretic form has been found in larvae and adults.