Supported by strand-specific RNA-Seq data.
Gene model reviewed during 5.46
There is only one protein coding transcript and one polypeptide associated with this gene
183 (aa); 19.3 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pig1 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Pig1 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Three adjacent segments located within the short region between Sgs4 and Pig1 can account for the switch from Pig1 expression during late second to early third instar to Sgs4 expression at the end of third instar. Germline transformation studies reveal that a 170bp segment acts as an enhancer to direct Sgs4 expression, a 64bp sequence just upstream can modify the temporal specificity of the enhancer so it works throughout the third instar and just further upstream is a repressor element.
Pig1 was molecularly characterized by the use of cDNA isolation and sequencing. The gene is devoid of introns and can differentially use two alternative 3' and 5' ends.
The br wild type gene product, which is necessary for the activation of intermoult glue genes, is necessary for the inactivation of the pre-moult genes.