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Gene Dmel\pim

General Information
Symbol	Dmel\pim	Species	D. melanogaster
Name	pimples	Annotation symbol	CG5052
Feature type	protein_coding_gene	FlyBase ID	FBgn0003087
Gene Model Status	Current	Stock availability	7 publicly available
Genomic Location
Chromosome (arm)	2L	Recombination map	2-30
Cytogenetic map	31D10-31D10	Sequence location	2L:10,362,989..10,364,046 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene pimples is referred to in FlyBase by the symbol Dmel\pim (CG5052, FBgn0003087). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence for 12 unique biological process terms, many of which group under: anatomical structure development; cellular component organization or biogenesis; cell cycle; cell cycle process; multicellular organismal process; nuclear division; cell division; epithelial cell differentiation; organ development; embryonic hindgut morphogenesis; mesoderm development. 26 alleles are reported. The phenotypes of these alleles are annotated with: organ system; organ system subdivision; portion of tissue; external compound sense organ; somatic cell; nuclear part; embryonic head; cytoplasmic part; cell cycle; adult segment. It has one annotated transcript and one annotated polypeptide. Protein features are: Securin sister-chromatid separation inhibitor. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval trachea, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1634209_at partially aligns to an exonic region of the only FlyBase-annotated transcript isoform of pim: 12 out of 13 probes fall into this exonic region. Gene sequence location is 2L:10362989..10364046.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	pim: pimples Homozygous lethal in embryo. Cuticle poorly differentiated with necrotic patches. Head abnormal.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593
FB2012_01	Sequence features BKN26514 HFA03591
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 31D10-31D10   Limits computationally determined from genome sequence between P{lacW}RnrLk06709 and P{lacW}KdelRk00311
Experimentally Determined Cytological Location
Cytogenetic map Notes References 31D-31D     (Lehner, 1995.11.29)
Experimentally Determined Recombination Data
Location	2-30 (Tearle and Nusslein-Volhard, 1987)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\pim for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) pim-RA FBtr0080001  NM_057330   932   199
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein pim-PA   FBpp0079591   22.7   199   4.67     NP_476678   AAF52925
Additional Polypeptide Data & Comments
Reported size (kDa)	199 (aa) (Stratmann and Lehner, 1996)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Securin sister-chromatid separation inhibitor (IPR006940)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA440623   AA536287   AE014134 AAF52925   AC007451   AW942430   AY061227 AAL28775   BI166356   BI167216   BI169929   BI216043   BI231480   BI233239   BI242431   BI358146   BI483434   CO154980   CO277461   CO283308   CO294318   CO305031   CO310036   CO311888   CO315520   CO329947   CO341928   EC064970   EC077206   EC078904   EC089278   EC201438   EC220636   EC225048   EC233973   EC255268   EC267445   EV588700   EV592842   GH748100   GH771208   GH773470   GH780491   GH798234   GH807735   GH817382   GH817575   GH828598   GH829429   GH831580   GH836392   GH837837   GH847131   GH871738   GH873342   GH875684   GH875913   GH891502   GH897637   GH903756   GH909528   GH912770   GH915577   GH935750   X93653 CAA63811
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q24454
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   presumptive embryonic/larval central nervous system   (Stratmann and Lehner, 1996) embryonic stage | early   organism | ubiquitous   (Stratmann and Lehner, 1996) embryonic stage & pupal stage & adult stage     (Stratmann and Lehner, 1996) third instar larval stage     (Stratmann and Lehner, 1996)
Additional Descriptive Data
	pim transcripts are detected in embryos, third instar larvae, and pupae. By in situ hybridization, pim transcripts are seen distributed throughout the embryo up to embyronic cycle 16. At later stages they become restricted to the developing nervous system and appear to be correlated with mitotic proliferation. (Stratmann and Lehner, 1996)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
	pim protein accumulates in the cytoplasm in interphase 14. Signals are present throughout the cell in prophase and metaphase 14, decrease in anaphase and are undetectable in telophase. pim protein also gradually accumulates during interphase and rapidly degrades during mitosis in later cell cycles. (Stratmann and Lehner, 1996)
Marker for
Subcellular Localization
CV Term	mitotic cycle (Stratmann and Lehner, 1996)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0003087    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0003087 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0003087 expression level Developmental Stage   Expression Level embryo 00-02hr    5488 embryo 02-04hr    4769 embryo 04-06hr    3873 embryo 06-08hr    2396 embryo 08-10hr    2745 embryo 10-12hr    1303 embryo 12-14hr    1725 embryo 14-16hr    575 embryo 16-18hr    1110 embryo 18-20hr    297 embryo 20-22hr    1935 embryo 22-24hr    1934 larva L1    21 larva L2    52 larva L3 12hr old    47 larva L3 puffstage 1-2    269 larva L3 puffstage 3-6    387 larva L3 puffstage 7-9    622 white prepupae new    505 white prepupae 12hr    906 white prepupae 24hr    717 pupae 2d postWPP    534 pupae 3d postWPP    325 pupae 4d postWPP    145 adult male 01day    362 adult male 05day    489 adult male 30day    284 adult female 01day    297 adult female 05day    2533 adult female 30day    2238 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (5488) embryo 02-04hr  (4769) embryo 04-06hr  (3873) embryo 06-08hr  (2396) embryo 08-10hr  (2745) embryo 10-12hr  (1303) embryo 12-14hr  (1725) embryo 14-16hr  (575) embryo 16-18hr  (1110) embryo 18-20hr  (297) embryo 20-22hr  (1935) embryo 22-24hr  (1934) larva L1    21 larva L2    52 larva L3 12hr old    47 larva L3 puffstage 1-2  (269) larva L3 puffstage 3-6  (387) larva L3 puffstage 7-9  (622) white prepupae new  (505) white prepupae 12hr  (906) white prepupae 24hr  (717) pupae 2d postWPP  (534) pupae 3d postWPP  (325) pupae 4d postWPP  (145) adult male 01day  (362) adult male 05day  (489) adult male 30day  (284) adult female 01day  (297) adult female 05day  (2533) adult female 30day  (2238) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (5488) embryo 02-04hr  (4769) embryo 04-06hr  (3873) embryo 06-08hr  (2396) embryo 08-10hr  (2745) embryo 10-12hr    1303 embryo 12-14hr  (1725) embryo 14-16hr    575 embryo 16-18hr    1110 embryo 18-20hr    297 embryo 20-22hr  (1935) embryo 22-24hr  (1934) larva L1    21 larva L2    52 larva L3 12hr old    47 larva L3 puffstage 1-2    269 larva L3 puffstage 3-6    387 larva L3 puffstage 7-9    622 white prepupae new    505 white prepupae 12hr    906 white prepupae 24hr    717 pupae 2d postWPP    534 pupae 3d postWPP    325 pupae 4d postWPP    145 adult male 01day    362 adult male 05day    489 adult male 30day    284 adult female 01day    297 adult female 05day  (2533) adult female 30day  (2238) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5488 embryo 02-04hr    4769 embryo 04-06hr    3873 embryo 06-08hr    2396 embryo 08-10hr    2745 embryo 10-12hr    1303 embryo 12-14hr    1725 embryo 14-16hr    575 embryo 16-18hr    1110 embryo 18-20hr    297 embryo 20-22hr    1935 embryo 22-24hr    1934 larva L1    21 larva L2    52 larva L3 12hr old    47 larva L3 puffstage 1-2    269 larva L3 puffstage 3-6    387 larva L3 puffstage 7-9    622 white prepupae new    505 white prepupae 12hr    906 white prepupae 24hr    717 pupae 2d postWPP    534 pupae 3d postWPP    325 pupae 4d postWPP    145 adult male 01day    362 adult male 05day    489 adult male 30day    284 adult female 01day    297 adult female 05day    2533 adult female 30day    2238 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5488 embryo 02-04hr    4769 embryo 04-06hr    3873 embryo 06-08hr    2396 embryo 08-10hr    2745 embryo 10-12hr    1303 embryo 12-14hr    1725 embryo 14-16hr    575 embryo 16-18hr    1110 embryo 18-20hr    297 embryo 20-22hr    1935 embryo 22-24hr    1934 larva L1    21 larva L2    52 larva L3 12hr old    47 larva L3 puffstage 1-2    269 larva L3 puffstage 3-6    387 larva L3 puffstage 7-9    622 white prepupae new    505 white prepupae 12hr    906 white prepupae 24hr    717 pupae 2d postWPP    534 pupae 3d postWPP    325 pupae 4d postWPP    145 adult male 01day    362 adult male 05day    489 adult male 30day    284 adult female 01day    297 adult female 05day    2533 adult female 30day    2238 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0003087 expression level Developmental Stage   Expression Level embryo 00-02hr    5488 embryo 02-04hr    4769 embryo 04-06hr    3873 embryo 06-08hr    2396 embryo 08-10hr    2745 embryo 10-12hr    1303 embryo 12-14hr    1725 embryo 14-16hr    575 embryo 16-18hr    1110 embryo 18-20hr    297 embryo 20-22hr    1935 embryo 22-24hr    1934 larva L1    21 larva L2    52 larva L3 12hr old    47 larva L3 puffstage 1-2    269 larva L3 puffstage 3-6    387 larva L3 puffstage 7-9    622 white prepupae new    505 white prepupae 12hr    906 white prepupae 24hr    717 pupae 2d postWPP    534 pupae 3d postWPP    325 pupae 4d postWPP    145 adult male 01day    362 adult male 05day    489 adult male 30day    284 adult female 01day    297 adult female 05day    2533 adult female 30day    2238 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (5488) embryo 02-04hr  (4769) embryo 04-06hr  (3873) embryo 06-08hr  (2396) embryo 08-10hr  (2745) embryo 10-12hr  (1303) embryo 12-14hr  (1725) embryo 14-16hr  (575) embryo 16-18hr  (1110) embryo 18-20hr  (297) embryo 20-22hr  (1935) embryo 22-24hr  (1934) larva L1    21 larva L2    52 larva L3 12hr old    47 larva L3 puffstage 1-2  (269) larva L3 puffstage 3-6  (387) larva L3 puffstage 7-9  (622) white prepupae new  (505) white prepupae 12hr  (906) white prepupae 24hr  (717) pupae 2d postWPP  (534) pupae 3d postWPP  (325) pupae 4d postWPP  (145) adult male 01day  (362) adult male 05day  (489) adult male 30day  (284) adult female 01day  (297) adult female 05day  (2533) adult female 30day  (2238) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (5488) embryo 02-04hr  (4769) embryo 04-06hr  (3873) embryo 06-08hr  2396 embryo 08-10hr  (2745) embryo 10-12hr    1303 embryo 12-14hr  1725 embryo 14-16hr    575 embryo 16-18hr    1110 embryo 18-20hr    297 embryo 20-22hr  1935 embryo 22-24hr  1934 larva L1    21 larva L2    52 larva L3 12hr old    47 larva L3 puffstage 1-2    269 larva L3 puffstage 3-6    387 larva L3 puffstage 7-9    622 white prepupae new    505 white prepupae 12hr    906 white prepupae 24hr    717 pupae 2d postWPP    534 pupae 3d postWPP    325 pupae 4d postWPP    145 adult male 01day    362 adult male 05day    489 adult male 30day    284 adult female 01day    297 adult female 05day  (2533) adult female 30day  2238 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5488 embryo 02-04hr    4769 embryo 04-06hr    3873 embryo 06-08hr    2396 embryo 08-10hr    2745 embryo 10-12hr    1303 embryo 12-14hr    1725 embryo 14-16hr    575 embryo 16-18hr    1110 embryo 18-20hr    297 embryo 20-22hr    1935 embryo 22-24hr    1934 larva L1    21 larva L2    52 larva L3 12hr old    47 larva L3 puffstage 1-2    269 larva L3 puffstage 3-6    387 larva L3 puffstage 7-9    622 white prepupae new    505 white prepupae 12hr    906 white prepupae 24hr    717 pupae 2d postWPP    534 pupae 3d postWPP    325 pupae 4d postWPP    145 adult male 01day    362 adult male 05day    489 adult male 30day    284 adult female 01day    297 adult female 05day    2533 adult female 30day    2238 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5488 embryo 02-04hr    4769 embryo 04-06hr    3873 embryo 06-08hr    2396 embryo 08-10hr    2745 embryo 10-12hr    1303 embryo 12-14hr    1725 embryo 14-16hr    575 embryo 16-18hr    1110 embryo 18-20hr    297 embryo 20-22hr    1935 embryo 22-24hr    1934 larva L1    21 larva L2    52 larva L3 12hr old    47 larva L3 puffstage 1-2    269 larva L3 puffstage 3-6    387 larva L3 puffstage 7-9    622 white prepupae new    505 white prepupae 12hr    906 white prepupae 24hr    717 pupae 2d postWPP    534 pupae 3d postWPP    325 pupae 4d postWPP    145 adult male 01day    362 adult male 05day    489 adult male 30day    284 adult female 01day    297 adult female 05day    2533 adult female 30day    2238 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0003087    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0003087 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval trachea, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0003087 expression level Tissue   Expression Level Larval Central Nervous System    305.325 Larval Midgut no informative data Larval Hindgut    20.6 Larval Malpighian Tubules    6.8 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    109.65 Larval Carcass    44.8 Adult Head no informative data Adult Eye    14.875 Adult Brain    5.7 Adult Thoracic-Abdominal Ganglion    5.2 Adult Crop no informative data Adult Midgut    7.2 Adult Hindgut    8.2 Adult Malpighian Tubules    5 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    5.475 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    9.5 Adult Ovary    1188.5 Adult Testis    289.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    305.325 Larval Midgut no informative data Larval Hindgut    20.6 Larval Malpighian Tubules    6.8 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    109.65 Larval Carcass    44.8 Adult Head no informative data Adult Eye    14.875 Adult Brain    5.7 Adult Thoracic-Abdominal Ganglion    5.2 Adult Crop no informative data Adult Midgut    7.2 Adult Hindgut    8.2 Adult Malpighian Tubules    5 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    5.475 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    9.5 Adult Ovary  (1188.5) Adult Testis    289.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    305.325 Larval Midgut no informative data Larval Hindgut    20.6 Larval Malpighian Tubules    6.8 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    109.65 Larval Carcass    44.8 Adult Head no informative data Adult Eye    14.875 Adult Brain    5.7 Adult Thoracic-Abdominal Ganglion    5.2 Adult Crop no informative data Adult Midgut    7.2 Adult Hindgut    8.2 Adult Malpighian Tubules    5 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    5.475 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    9.5 Adult Ovary  (1188.5) Adult Testis    289.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    305.325 Larval Midgut no informative data Larval Hindgut    20.6 Larval Malpighian Tubules    6.8 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    109.65 Larval Carcass    44.8 Adult Head no informative data Adult Eye    14.875 Adult Brain    5.7 Adult Thoracic-Abdominal Ganglion    5.2 Adult Crop no informative data Adult Midgut    7.2 Adult Hindgut    8.2 Adult Malpighian Tubules    5 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    5.475 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    9.5 Adult Ovary    1188.5 Adult Testis    289.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0003087 expression level Tissue   Expression Level Larval Central Nervous System    305.325 Larval Midgut no informative data Larval Hindgut    20.6 Larval Malpighian Tubules    6.8 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    109.65 Larval Carcass    44.8 Adult Head no informative data Adult Eye    14.875 Adult Brain    5.7 Adult Thoracic-Abdominal Ganglion    5.2 Adult Crop no informative data Adult Midgut    7.2 Adult Hindgut    8.2 Adult Malpighian Tubules    5 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    5.475 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    9.5 Adult Ovary    1188.5 Adult Testis    289.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    305.325 Larval Midgut no informative data Larval Hindgut    20.6 Larval Malpighian Tubules    6.8 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    109.65 Larval Carcass    44.8 Adult Head no informative data Adult Eye    14.875 Adult Brain    5.7 Adult Thoracic-Abdominal Ganglion    5.2 Adult Crop no informative data Adult Midgut    7.2 Adult Hindgut    8.2 Adult Malpighian Tubules    5 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    5.475 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    9.5 Adult Ovary  (1188.5) Adult Testis    289.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    305.325 Larval Midgut no informative data Larval Hindgut    20.6 Larval Malpighian Tubules    6.8 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    109.65 Larval Carcass    44.8 Adult Head no informative data Adult Eye    14.875 Adult Brain    5.7 Adult Thoracic-Abdominal Ganglion    5.2 Adult Crop no informative data Adult Midgut    7.2 Adult Hindgut    8.2 Adult Malpighian Tubules    5 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    5.475 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    9.5 Adult Ovary  1188.5 Adult Testis    289.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    305.325 Larval Midgut no informative data Larval Hindgut    20.6 Larval Malpighian Tubules    6.8 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    109.65 Larval Carcass    44.8 Adult Head no informative data Adult Eye    14.875 Adult Brain    5.7 Adult Thoracic-Abdominal Ganglion    5.2 Adult Crop no informative data Adult Midgut    7.2 Adult Hindgut    8.2 Adult Malpighian Tubules    5 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    5.475 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    9.5 Adult Ovary    1188.5 Adult Testis    289.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea     Larval Carcass     Adult Head no informative data Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop no informative data Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    no informative data   NA  Eye    14.875   NA  Brain    5.7   305.325  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    5.2   NA  Crop    no informative data   no informative data  Midgut    7.2   20.6  Hindgut    8.2   6.8  Malpighian Tubules    5   no informative data  Fat Body    no informative data   no informative data  Salivary Gland    no informative data   NA  Heart    5.475   109.65  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    9.5   NA  Ovary    1188.5   NA  Testis    289.6   NA  Male Accessory Gland    no informative data   44.8  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_01
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003087 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5052-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal pimg.dba.T:Hsap\MYC lethal, with Scer\GAL41407 pimGD11516 lethal | embryonic stage | recessive pimIL lethal | recessive pim2 pim3 pim4 pimIL viable, with Scer\GAL4pnr-MD237 pimGD11516 Sterility Allele sterile pimg.dba.T:Hsap\MYC Other Phenotypes Allele cytokinesis defective pimIL increased cell death pimIL mitotic cell cycle defective pimIL mitotic cell cycle defective | embryonic cycle 15 pimIL mitotic cell cycle defective | embryonic stage 14 pimkenadba.g.T:Hsap\MYC mitotic cell cycle defective | embryonic stage 14, with Scer\GAL4nos.bcd3'UTR.T:Scer\GCN4 pimkena.Scer\UAS.T:Hsap\MYC mitotic cell cycle defective | recessive pim2 pim3 pim4 pimIL neuroanatomy defective | larval stage, with Scer\GAL41407 pimGD11516 visible, with Scer\GAL4GMR.PF pimScer\UAS.T:Hsap\MYC wild-type pim+tSa pimhs.PS Phenotype manifest in Allele centrosome pimIL embryonic/first instar larval cuticle pimIL embryonic/larval neuroblast | larval stage, with Scer\GAL41407 pimGD11516 embryonic head pimIL embryonic Malpighian tubule pimIL epidermal cell pimIL epithelial cell pimIL eye pimg.dba.T:Hsap\MYC eye, with Scer\GAL4GMR.PF pimScer\UAS.T:Hsap\MYC ganglion mother cell | larval stage, with Scer\GAL41407 pimGD11516 gonad | embryonic stage (with pim66.37) pim77.45 gonad | embryonic stage (with pim77.45) pim66.37 gonadal sheath proper primordium | embryonic stage (with pim66.37) pim77.45 gonadal sheath proper primordium | embryonic stage (with pim77.45) pim66.37 Malpighian tubule pimIL metaphase plate pimIL metaphase plate, with Scer\GAL4nos.bcd3'UTR.T:Scer\GCN4 pimkena.Scer\UAS.T:Hsap\MYC M phase of mitotic cell cycle, with Scer\GAL4da.G32 pimdba.Scer\UAS.T:Hsap\MYC M phase of mitotic cell cycle, with Scer\GAL4nos.bcd3'UTR.T:Scer\GCN4 pimdba.Scer\UAS.T:Hsap\MYC nuclear chromosome, with Scer\GAL4da.G32 pimdba.Scer\UAS.T:Hsap\MYC nuclear chromosome, with Scer\GAL4nos.bcd3'UTR.T:Scer\GCN4 pimdba.Scer\UAS.T:Hsap\MYC ommatidium pimg.dba.T:Hsap\MYC ommatidium, with Scer\GAL4GMR.PF pimScer\UAS.T:Hsap\MYC visceral mesoderm | embryonic stage (with pim66.37) pim77.45 visceral mesoderm | embryonic stage (with pim77.45) pim66.37 wing pimg.dba.T:Hsap\MYC
Classical Alleles ( 10 )
For All Classical Alleles Show
Allele of pim Class Mutagen Stocks Known lesion pimIL amorphic allele - genetic evidence ethyl methanesulfonate 2 Yes pimA385 2 -- pim2 amorphic allele - genetic evidence X ray 0 Yes pim3 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes pim4 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes pim66.37 ethyl methanesulfonate 0 -- pim77.45 ethyl methanesulfonate 0 -- pimIR46 0 -- pimIR47 0 -- pimunspecified 0 --
Alleles Carried on Transgenic Constructs ( 16 )
For All Alleles Carried on Transgenic Constructs Show
Allele of pim Class Mutagen Stocks Known lesion pimGD11516 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes pimKK106514 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pim+tSa in vitro construct - genomic fragment 0 Yes pim2.Scer\UAS.T:Hsap\MYC in vitro construct - deletion 0 Yes pimdba.Scer\UAS.T:Hsap\MYC in vitro construct - site directed mutagenesis 0 Yes pimg.dba.T:Hsap\MYC in vitro construct - coding region fusion in vitro construct - site directed mutagenesis 0 Yes pimg.Scer\FRT in vitro construct - regulatory fusion 0 Yes pimg FLPase 0 Yes pimhs.PS in vitro construct - regulatory fusion 0 Yes pimkena.Scer\UAS.T:Hsap\MYC in vitro construct - site directed mutagenesis 0 Yes pimkenadba.g.Scer\FRT.T:Hsap\MYC in vitro construct - coding region fusion 0 Yes pimkenadba.g.Scer\FRT in vitro construct - regulatory fusion 0 Yes pimkenadba.g.T:Hsap\MYC FLPase 0 Yes pimkenadba.g FLPase 0 Yes pimScer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion 0 Yes pimT:Hsap\MYC in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)G2 (Clegg et al., 1993) Df(2L)J2 (Clegg et al., 1993, Smith et al., 1993) Df(2L)J3 (Clegg et al., 1993) Df(2L)J16 (Clegg et al., 1993) Df(2L)J17 (Clegg et al., 1993) Df(2L)J27 (Nusslein-Volhard et al., 1984, Tearle and Nusslein-Volhard, 1987, Clegg et al., 1993) Df(2L)J39 (Clegg et al., 1993) Df(2L)J77 (Clegg et al., 1993) Df(2L)J106 (Clegg et al., 1993, Stratmann and Lehner, 1996)
Not disrupted in	Df(2L)J1 (Clegg et al., 1993)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11516} NA P{KK106514} NA P{UAS-pim.kena.myc} NA P{UAS-pim2-myc} NA P{UAS-pimdba-myc} NA P{UAS-pim-myc} NA heat-shock construct P{Hs-pim} NA characterization construct P{g(FRT.STOP)pim.kenadba.MYC} NA P{g(FRT.STOP)pim.kenadba} NA P{g(FRT.STOP)pim} NA P{g(-FRT)pim.kenadba.MYC} NA P{g(-FRT)pim.kenadba} NA P{g(-FRT)pim} NA P{gpimdba-myc} NA P{pim+} NA P{PIM-MYC} NA P{UAS-CycB-dbapim} NA P{UAS-CycB-dbpim} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{5HPw+}pimA385 NA
Gene Ontology: Function, Process & Cellular Component ( 15 unique terms )
Terms Based on Experimental Evidence ( 13 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
centrosome duplication inferred from mutant phenotype (Pandey et al., 2005) cytokinesis inferred from mutant phenotype (Pandey et al., 2005) digestive tract mesoderm development inferred from mutant phenotype (Weyers et al., 2011) epithelial cell development inferred from mutant phenotype (Pandey et al., 2005) gonad development inferred from mutant phenotype (Weyers et al., 2011) Malpighian tubule morphogenesis inferred from mutant phenotype (Jack and Myette, 1999) mitotic anaphase inferred from mutant phenotype (Leismann and Lehner, 2003) mitotic sister chromatid segregation inferred from mutant phenotype (Leismann et al., 2000) mitotic sister chromatid separation inferred from mutant phenotype (Pandey et al., 2005) mitotic telophase inferred from mutant phenotype (Leismann and Lehner, 2003) nervous system development inferred from mutant phenotype (Koizumi et al., 2007) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (assigned by UniProtKB) (Herzig et al., 2002)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
DNA metabolic process inferred from electronic annotation with InterPro:IPR006940 (FlyBase Curators et al., 2004-) mitotic sister chromatid segregation non-traceable author statement (Gonzalez and Bornens, 2000) traceable author statement (Lee and Orr-Weaver, 2003)
Cellular Component
CV term Evidence References
nucleus inferred from electronic annotation with InterPro:IPR006940 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions pim allele Gene References pimScer\UAS.T:Hsap\MYC fzy (Leismann et al., 2000) unspecified Cenp-C (Heeger et al., 2005) CycB (Ji et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60489 DroID - A comprehensive database of gene and protein interactions. • FBgn0003087 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34404
Orthologs
Genome-wide drosophilid orthologs	Dana\GF15772 Dere\pim Dgri\GH13880 Dmoj\GI10136 Dper\GL19150 Dpse\pim Dsec\GM18236 Dsim\GD23684 Dvir\GJ18371 Dyak\pim
Curated drosophilid orthologs	Dere\pim   Dore\pim   Dpse\pim   Dyak\pim
Linkouts	OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0067426 FBgn0205530 FBgn0195061 FBgn0173143 FBgn0067543 FBgn0156749 FBgn0132902 FBgn0121356 FBgn0120889 FBgn0118462 FBgn0067597 FBgn0092796
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	3117 pimIL cn1 bw1 sp1/CyO 16103 y1 w1118; PBac{5HPw+}pimA385/CyO
Kyoto	106857 pimIL cn1 bw1 sp1/CyO 111719 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; PBac{5HPw+}pimA385 P{neoFRT}40A/CyO y+
VDRC	v100534 P{KK106514}VIE-260B v40571 w1118; P{GD11516}v40571/CyO v40573 w1118; P{GD11516}v40573/TM3
Genomic Clones ( 2 )
	BACR30M19 BACR48K04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 58 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD16810
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD15464 RE06459 RE07552 RE11246 RE21951 RE22845 RE29535 RE39756 RE44830 RE66333
Other clones	6098 43538 76431 97653 103759 124714 158868 191722 291442 Dmel_neb_013628_0585_3486 Dmel_neb_014172_0081_1101 EC255268 EK124743 EK162824 EK206547 EK220310 EK227137 EK247113 EK261732 EK294227 EN05136 EP13440 RP003023782 RP003036157 RP003047493 RP003077032 RP003103696 RP003156603 RP003190674 RP003194869 RP003201512 RP003237725 RP003272176 RP003281196 RP003335757 RP003336480 RP003339801 RP003346280 RP003379113 RP003427459 RP003465764 RP003483332 RP003542001 RP003597121 RP003601616 RP003633036 RP003677538
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003087 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34404
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	pim and thr are required for sister chromatin separation in mitosis. (Leismann et al., 2000) There is no synergistic interaction between pim and thr. (Philp and Glover, 1997) The metaphase arrest seen in fzy mutants persists in the absence of pim or thr function. (Philp and Glover, 1997) pim is required for separation of sister chromatids in mitosis. (Stratmann and Lehner, 1996) Five (unnamed) recessive lethal alleles have been isolated during a cytogenetic analysis of chromosomal region 31. (Clegg et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057330 RefSeq (Proteins) • NP_476678 DDBJ / EMBL / GenBank • AA536287 AAF52925 AAL28775 AC007451 AW942430 AY061227 CAA63811 X93653 Entrez Gene - A searchable database of RefSeq genes. • 34404 UniProtKB/TrEMBL • Q24454
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Securin sister-chromatid separation inhibitor (IPR006940)
Linkouts
	BioGRID - A database of protein and genetic interactions • 60489 DroID - A comprehensive database of gene and protein interactions. • FBgn0003087 DRSC - Results from RNAi screens. • FBgn0003087 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003087 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5052-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003087 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34404 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /newgene/pimples1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34404 modMine - Data generated by the modENCODE project. • FBgn0003087 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0067426 FBgn0205530 FBgn0195061 FBgn0173143 FBgn0067543 FBgn0156749 FBgn0132902 FBgn0121356 FBgn0120889 FBgn0118462 FBgn0067597 FBgn0092796
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	CG5052 (Keleman et al., 2009.8.5) frag2 (Weyers et al., 2011) l(2)31Df   PIM (Ross and Cohen-Fix, 2002, Heeger et al., 2003, Lehner, 2003, Nagao and Yanagida, 2002, Heidmann et al., 2001, Pesin and Orr-Weaver, 2007) pim (Liu et al., 2005, Hueber et al., 2007, Georlette et al., 2007, Pesin and Orr-Weaver, 2007, Koizumi et al., 2007, Pandey et al., 2005, Leismann and Lehner, 2003, Magalhaes et al., 2007, Weyers et al., 2011, Neumüller et al., 2011)
Name Synonym	fragmented gonads 2 (Weyers et al., 2011) pimples (Lehner, 1995.11.29, Liu et al., 2005, Pandey et al., 2005, Weyers et al., 2011) PIMPLES (Pesin and Orr-Weaver, 2007) securin (Leismann and Lehner, 2003, Oliveira et al., 2010, Li et al., 2010)
Secondary FlyBase IDs
References ( 76 )
Generate a list of	All references relating to this gene ( 76 )
List References by type	Research paper  ( 31 ) Supplementary material  ( 4 ) Review  ( 19 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 10 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Conference report  ( 2 ) Computer file  ( 1 )
Recent research papers ( 5 )
	Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Weyers et al., 2011, Dev. Biol. 353(2): 217--228 A genetic screen for mutations affecting gonad formation in Drosophila reveals a role for the slit/robo pathway. [FBrf0213539] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Li et al., 2010, Mol. Cell. Biol. 30(13): 3384--3395 Recruitment of Cdc20 to the kinetochore requires BubR1 but not Mad2 in Drosophila melanogaster. [FBrf0210988] Oliveira et al., 2010, Nat. Cell Biol. 12(2): 185--192 Cohesin cleavage and Cdk inhibition trigger formation of daughter nuclei. [FBrf0209876] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...