Gene Dmel\pk

General Information
Symbol	Dmel\pk	Species	D. melanogaster
Name	prickle	Annotation symbol	CG11084
Feature type	protein_coding_gene	FlyBase ID	FBgn0003090
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	2R	Recombination map	2-55.3
Cytogenetic map	42F3-43A1	Sequence location	2R:3,038,207..3,110,885 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene prickle is referred to in FlyBase by the symbol pk (CG11084, FBgn0003090). It has the cytological map location 42F3-43A1. Its sequence location is 2R:3038207..3110885. Its molecular function is described as: protein binding; zinc ion binding. It is involved in the biological processes: establishment of ommatidial polarity; establishment and/or maintenance of cell polarity; establishment of planar polarity; protein localization; establishment of protein localization; maintenance of protein localization; establishment of imaginal disc-derived wing hair orientation. 90 alleles are reported. The phenotypes of these alleles are annotated with 18 unique terms, many of which group under: adult segment; peripheral nervous system; thoracic segment; nervous system; appendage segment; adult mesothoracic segment; organ system; metatarsus; wing hair; adult. It has 3 annotated transcripts and 3 annotated polypeptides.
External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	pk: prickle Polarity pattern of wing, haltere, and notum altered in mutants. Chaetae of triple row on anterior wing slanted anteriorly instead of posteriorly. Trichomes near L2 vein arranged in counterclockwise whorl on right wing blade, in clockwise whorl on left blade; trichomes in anterior wing occasionally duplicated (Gubb and Garcia-Bellido, 1982). On the notum, the posterior acrostichals are irregularly erect and whorled. Occasionally, extra dorsocentral and scutellar bristles appear at temperatures above 23. Mutant flies slightly larger than wild type. Gubb and Garcia-Bellido (1982) describe somatic clones of homozygous pk cells. sple: spiny legs Polarity of chaetae and trichomes on legs irregular; relations between bracts and bristles disrupted. High incidence of ectopic tarsal joints with inverted polarity, especially in tarsae 3 and 4; incomplete intersegmental membranes between tarsal segments, especially between segments 3 and 4; no extra sensilla companiformia despite extra joints. Chaetae on abdominal tergites turned toward midline instead of pointing posteriorly as in wild type; polarity of bristles on sternites disrupted as well. sple3 A low-temperature-sensitive allele. Legs of flies raised at 19 highly condensed with incomplete joints, haphazard bristle pattern, occasional increase in number of bristle rows, and absence of some prominent markers. Flies unable to walk and soon get stuck in food. In sple3 flies raised at 28 53% of legs, though apparently normal, display abnormalities such as swollen second, third, and fourth tarsal segments, abnormal tarsal joints and reversals in bristle orientation in the middle of every segment; such flies able to walk and breed. About 30% of legs show phenotype intermediate between the two phenotypes described above. Mutant phenotype expressed in the homeotic legs of Antp and ssa; also expressed in mitotic clones.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 42F3-43A1 Limits computationally determined from genome sequence between P{lacW}vimar^k16722 and P{lacW}cos^k16101
Experimentally Determined Cytological Location
Cytogenetic map Notes References 43A1-43A2 (Gubb, 1999.7.5) 42E3-43C3 Placed in 42E3--43C3 by deficiency mapping. This cytological map position does not agree with FBrf0020044. (Ashburner et al., 1981)
Experimentally Determined Recombination Data
Location	2-55.3 (Grau and Simpson, 1987) 2-56 (Ashburner et al., 1981) 2-53 (Craymer, 1980) 2-55 (Goldschmidt, 1944)
Left of (cM)	cn (Ashburner et al., 1981) pwn (Ashburner et al., 1981, Grau and Simpson, 1987)
Right of (cM)
Notes	Genetic map position is approximate. (Ashburner et al., 1981) Alleles of the 'prickle' phenotypic class map to the left of alleles of the 'sple' phenotypic class. (Grau and Simpson, 1987) The cellular polarity gene pk and the adult lethal nec gene map within the proximal region of pk-sple complex among a set of 3 Serpin transcripts. (Gubb and Green, 1995)
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: Spn43Aa+ pk+ Spn43Ab- nec- pk+ (Green et al., 2000) Gene Order (overall orientation not stated) References Overall orientation not stated: pk+ Spn43Ab- Spn43Ad? nec- pk+ (Gubb, 2001.3.5)
Gene Model & Products
Please see the GBrowse view of Dmel\pk for information on other features
Comments on Gene Model
	Transposons inserted in two introns
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) pk-RA FBtr0089042 NM_165508 4244 963 pk-RB FBtr0089043 NM_165509 4093 1029 pk-RC FBtr0089044 NM_165512 5197 1299
Additional Transcript Data & Comments
Reported size (kB)	5.1, 4.2 (northern blot) (Gubb et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein pk-PA FBpp0088113 104.7 963 7.23 NP_724534 AAM68908 pk-PB FBpp0088114 111.8 1029 6.94 NP_724535 AAF59284 pk-PC FBpp0088115 140.7 1299 6.87 NP_724538 AAF59281
Additional Polypeptide Data & Comments
Reported size (kD)	1206, 936, 870 (aa) (Gubb et al., 1999)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0003090
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, LIM-type (IPR001781) Zinc finger, C2H2-type (IPR007087) PET (IPR010442)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003842 AAF59281 AE003842 AAF59284 AE003842 AAM68908 AI294674 AI296484 AI513684 AI533454 AI534426 AI541728 AJ243708 CAB57344 AJ243709 CAB99211 AJ243710 CAB57345 AQ254872 AY051420 AY060622 BF487160 BF487258 BF487579 BF488004 BF488518 BF488573 BF490004 BF490395 BF491607 BF491634 BF492027 BF492416 BF493692 BF493816 BF494757 BF495630 BF495646 BF496164 BF497290 BF497365 BF498229 BF499442 BF500330 BF500862 BF501798 BF502006 BF503557 BF503791 BF504399 BF504438 BF505736 BF506499 BG634799 BG634868 BG635266 BG635313 BG635408 BI632577 BT001301 BT001339 CK605300 CK605301 CO182238 CO188193 CO195450 CO275031 CO305638 CO312438 CO322905 CO333512 CO342771 CZ472184 CZ486664 CZ486665 CZ489851 DN154343 DQ017440 AAY82222 DQ017441 AAY82223 EC054349 EC205374 EC209673 EC220291 EC225092 EC249281 EC252343 EC254318 EC259329 EL870169 SD05264 UT01823

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q3YNB2 A1Z6W3

Maps to
Does NOT map to
Identified with
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 13562
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference pupal stage \| 28-34hr wing (Gubb et al., 1999) embryonic stage ventral midline (Gubb et al., 1999) larval stage imaginal disc (Gubb et al., 1999) larval stage \| third instar morphogenetic furrow \| posterior to (Gubb et al., 1999) pupal stage \| 28-34hr leg (Gubb et al., 1999) embryonic stage cephalic furrow (Gubb et al., 1999) larval stage dorsal mesothoracic disc (Gubb et al., 1999) larval stage eye disc (Gubb et al., 1999) larval stage imaginal disc (Gubb et al., 1999) embryonic stage ventral midline (Gubb et al., 1999) embryonic stage cephalic furrow (Gubb et al., 1999) pupal stage \| 28-34hr wing (Gubb et al., 1999) larval stage dorsal mesothoracic disc (Gubb et al., 1999) larval stage eye disc (Gubb et al., 1999) pupal stage \| 28-34hr leg (Gubb et al., 1999) larval stage \| third instar morphogenetic furrow \| posterior to (Gubb et al., 1999)
Marker for

Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for

Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003090 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0003090 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0003090
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable pk⁸⁷ pk^GO12 pk^f04137 pk^f07080 Other Phenotypes Allele visible pk^D pk^pk-sple-13 pk^sple-1 visible \| recessive pk¹⁵ pk¹⁶ pk¹⁷ pk¹⁹ pk¹ pk²⁰ pk²¹ pk² pk³⁰ pk³ pk⁴⁴ pk⁵ pk^GO12 pk^pk-sple-10 pk^pk-sple-11 pk^pk-sple-12 pk^pk-sple-13 pk^pk-sple-14 pk^pk-sple-18 pk^pk-sple-23 pk^pk-sple-25 pk^pk-sple-26 pk^pk-sple-32 pk^pk-sple-33 pk^pk-sple-34 pk^pk-sple-35 pk^pk-sple-37 pk^pk-sple-38 pk^pk-sple-39 pk^pk-sple-40 pk^pk-sple-41 pk^pk-sple-43 pk^pk-sple-46 pk^pk-sple-47 pk^pk-sple-48 pk^pk-sple-6 pk^pk-sple-7 pk^pk-sple-8 pk^pk-sple-9 pk^sple-1 pk^sple-27 pk^sple-36 pk^sple-3 pk^sple-42 pk^sple-4 pk^sple-5 pk^unspecified visible \| recessive, with Scer\GAL4[-]: pk^EP2557 visible \| dominant pk^D1 visible \| conditional - cold sensitive pk^sple-3 visible, with Scer\GAL4^dpp.blk1 pk^Scer\UAS.cGa visible, with Scer\GAL4^Act pk^{sple.Scer\UAS} visual behavior defective pk^pk-sple-10 pk^pk-sple-14 pk^pk-sple-18 pk^pk-sple-25 pk^pk-sple-6 pk^pk-sple-7 pk^pk-sple-8 pk^pk-sple-9 cell polarity defective pk^pk-sple-13 pk^pk.sev cell polarity defective \| cell non-autonomous, with Scer\GAL4^Ubx.PdC pk^Scer\UAS.cGa cell polarity defective, with Scer\GAL4^ptc-559.1 pk^Scer\UAS.cGa pk^{sple.Scer\UAS} cell polarity defective, with Scer\GAL4^hs.2sev pk^{sple.Scer\UAS} tissue polarity defective pk¹⁵ pk¹⁶ pk¹⁷ pk¹⁹ pk¹ pk²⁰ pk²¹ pk²⁹ pk² pk³⁰ pk³ pk⁴⁴ pk⁵ pk^D pk^SZ4 pk^eq pk^nap2 pk^pk-sple-10 pk^pk-sple-11 pk^pk-sple-12 pk^pk-sple-13 pk^pk-sple-14 pk^pk-sple-18 pk^pk-sple-22 pk^pk-sple-23 pk^pk-sple-25 pk^pk-sple-26 pk^pk-sple-32 pk^pk-sple-33 pk^pk-sple-34 pk^pk-sple-35 pk^pk-sple-37 pk^pk-sple-38 pk^pk-sple-39 pk^pk-sple-40 pk^pk-sple-41 pk^pk-sple-43 pk^pk-sple-46 pk^pk-sple-47 pk^pk-sple-48 pk^pk-sple-49 pk^pk-sple-6 pk^pk-sple-7 pk^pk-sple-8 pk^pk-sple-9 pk^sple-1 pk^sple-27 pk^sple-36 pk^sple-3 pk^sple-42 pk^sple-4 pk^sple-5 pk^unspecified tissue polarity defective \| cell autonomous \| somatic clone pk¹ pk^pk-sple-13 tissue polarity defective \| cell non-autonomous pk¹ tissue polarity defective \| cell non-autonomous \| somatic clone pk¹ tissue polarity defective \| cell non-autonomous \| somatic clone, with Scer\GAL4^αTub84B.PL pk^{sple.Scer\UAS} tissue polarity defective \| recessive pk¹ pk²⁹ pk^78a pk^78d pk^78m pk^GO12 tissue polarity defective \| recessive, with Scer\GAL4[-]: pk^EP2557 tissue polarity defective \| dominant pk^TBJ21 tissue polarity defective \| somatic clone pk^pk-sple-13 tissue polarity defective \| somatic clone (with pk^sple-1) pk^pk-sple-13 tissue polarity defective \| somatic clone (with pk^pk-sple-13) pk^sple-1 tissue polarity defective \| somatic clone, with Scer\GAL4^αTub84B.PL pk^{sple.Scer\UAS} tissue polarity defective, with Scer\GAL4^ptc-559.1 pk^Scer\UAS.cGa tissue polarity defective, with Scer\GAL4^da.G32 pk^EP2557 pk^Scer\UAS.cGa pk^{sple.Scer\UAS} tissue polarity defective, with Scer\GAL4^C-765 pk^Scer\UAS.cGa tissue polarity defective, with Scer\GAL4^Act5C.PI pk^{sple.Scer\UAS} tissue polarity defective, with Scer\GAL4^αTub84B.PL pk^{sple.Scer\UAS} tissue polarity defective, with Scer\GAL4^Act pk^{sple.Scer\UAS} Sterility Allele fertile pk^f04137 pk^f07080 Phenotype manifest in Allele wing pk¹ pk¹⁵ pk¹⁶ pk¹⁷ pk¹⁹ pk² pk²⁰ pk²¹ pk²⁹ pk³ pk³⁰ pk⁴⁴ pk⁵ pk^SZ4 pk^eq pk^nap2 pk^pk-sple-10 pk^pk-sple-11 pk^pk-sple-12 pk^pk-sple-13 pk^pk-sple-14 pk^pk-sple-18 pk^pk-sple-22 pk^pk-sple-23 pk^pk-sple-25 pk^pk-sple-26 pk^pk-sple-32 pk^pk-sple-33 pk^pk-sple-34 pk^pk-sple-35 pk^pk-sple-37 pk^pk-sple-38 pk^pk-sple-39 pk^pk-sple-40 pk^pk-sple-41 pk^pk-sple-43 pk^pk-sple-46 pk^pk-sple-47 pk^pk-sple-48 pk^pk-sple-49 pk^pk-sple-6 pk^pk-sple-7 pk^pk-sple-8 pk^pk-sple-9 scutum pk¹ pk¹⁵ pk¹⁶ pk¹⁷ pk¹⁹ pk² pk²⁰ pk²¹ pk²⁹ pk³ pk³⁰ pk⁴⁴ pk⁵ pk^SZ4 pk^pk-sple-10 pk^pk-sple-11 pk^pk-sple-12 pk^pk-sple-13 pk^pk-sple-14 pk^pk-sple-18 pk^pk-sple-22 pk^pk-sple-23 pk^pk-sple-25 pk^pk-sple-26 pk^pk-sple-32 pk^pk-sple-33 pk^pk-sple-34 pk^pk-sple-35 pk^pk-sple-37 pk^pk-sple-38 pk^pk-sple-39 pk^pk-sple-40 pk^pk-sple-41 pk^pk-sple-43 pk^pk-sple-46 pk^pk-sple-47 pk^pk-sple-48 pk^pk-sple-49 pk^pk-sple-6 pk^pk-sple-7 pk^pk-sple-8 pk^pk-sple-9 triple row pk¹ pk^pk-sple-13 wing hair pk¹ pk²⁹ pk³⁰ pk^78a pk^78d pk^78m pk^D pk^nap2 pk^pk-sple-10 pk^pk-sple-13 pk^pk-sple-14 pk^pk-sple-18 pk^pk-sple-25 pk^pk-sple-6 pk^pk-sple-7 pk^pk-sple-8 pk^pk-sple-9 wing hair \| somatic clone pk¹ wing \| cell non-autonomous \| somatic clone pk¹ pk^pk-sple-13 wing \| cell autonomous \| somatic clone pk¹ pk^pk-sple-13 <