FlyBase Gene Report: Dmel\Pkc53E

General Information

Symbol

Dmel\Pkc53E

Species

D. melanogaster

Name

Protein C kinase 53E

Annotation Symbol

CG6622

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003091

Gene Model Status

Current

Stock Availability

16 publicly available

Enzyme Name (EC)

Protein kinase C (2.7.11.13)

Dual-specificity kinase (2.7.12.1)

Gene Summary

PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins (By similarity). PKC also serves as the receptor for phorbol esters, a class of tumor promoters (By similarity). Acts in a hh-signaling pathway which regulates the Duox-dependent gut immune response to bacterial uracil; required for the activation of Cad99C and consequently Cad99C-dependent endosome formation, which is essential for the Duox-dependent production of reactive oxygen species (ROS) in response to intestinal bacterial infection (PubMed:25639794). (UniProt, P05130)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

PKC, protein kinase C, dPKC, dPKC53E(br), Pkc1

Key Links

Genomic Location

Cytogenetic map

53E2-53E2

Sequence location

2R:16,924,655..16,945,126 [+]

Recombination map

2-81

RefSeq locus

NT_033778 REGION:16924655..16945126

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

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Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (11 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein serine/threonine kinase activity

inferred from direct assay

(Corbett et al., 1993)

enables protein serine/threonine/tyrosine kinase activity

inferred from mutant phenotype

(Xi et al., 2017)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR000961, InterPro:IPR002290, InterPro:IPR014375, InterPro:IPR017441, InterPro:IPR017892

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables protein kinase C activity

inferred from electronic annotation with InterPro:IPR014375

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Rosenthal et al., 1987)

enables protein serine/threonine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000683254

(assigned by GO_Central )

(Gaudet et al., 2011)

enables zinc ion binding

inferred from electronic annotation with InterPro:IPR014375

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in positive regulation of clathrin-dependent endocytosis

inferred from genetic interaction with FLYBASE:inaC; FB:FBgn0004784

(Lee et al., 2015)

involved_in protein phosphorylation

inferred from direct assay

(Corbett et al., 1993)

involved_in regulation of hemocyte proliferation

inferred from mutant phenotype

(Sinenko et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in intracellular signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN000683254

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in peptidyl-serine phosphorylation

inferred from biological aspect of ancestor with PANTHER:PTN000683254

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in plasma membrane

inferred from sequence or structural similarity with FLYBASE:inaC; FB:FBgn0004784

(FlyBase Curators, 2008-)

Gene Group (FlyBase)

PROTEIN KINASE C FAMILY

Protein Family (UniProt)

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. (P05130)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L- seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein] (2.7.12.1)

Predictions / Assertions

ATP + a protein = ADP + a phosphoprotein (2.7.11.13)

Summaries

Gene Group (FlyBase)

PROTEIN KINASE C FAMILY -: The Protein Kinase C (PKC) family are serine/threonine kinases activated by diacylglycerol (DAG), lipid or Ca²⁺. PKCs are regulated by auto-inhibition through a pseudosubstrate site located in the regulatory domain. (Adapted from PMID:20094051).

Protein Function (UniProtKB)

(UniProt, P05130)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Pkc1: Protein kinase-c1

Structural gene for protein kinase C (PKC) that is expressed primarily in the brain of adult flies.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Pkc53E for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P05130)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Pkc53E-RA

FBtr0087049

NM_057334

3175

670

Pkc53E-RB

FBtr0087048

NM_166201

2696

679

Pkc53E-RC

FBtr0112819

NM_001103880

2693

678

Pkc53E-RD

FBtr0305069

NM_001259448

2024

525

Pkc53E-RE

FBtr0305070

NM_001259449

2633

678

Pkc53E-RF

FBtr0331960

NM_001274106

4711

670

Additional Transcript Data and Comments

Reported size (kB)

4.3, 4.0, 2.4 (northern blot)

(Rosenthal et al., 1987)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Pkc53E-PA

FBpp0086198

76.7

670

7.15

NP_476682

AAF57932

Pkc53E-PB

FBpp0086197

77.7

679

7.14

NP_725626

AAF57933

Pkc53E-PC

FBpp0111731

77.7

678

7.67

NP_001097350

ABV53829

Pkc53E-PD

FBpp0293606

60.4

525

6.27

NP_001246377

AFH08130

Pkc53E-PE

FBpp0293607

77.7

678

7.67

NP_001246378

AFH08131

Pkc53E-PF

FBpp0304291

76.7

670

7.15

NP_001261035

AGB93567

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

670 aa isoforms: Pkc53E-PA, Pkc53E-PF

678 aa isoforms: Pkc53E-PC, Pkc53E-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

670 (aa)

(Schaeffer, 1998.11.23)

639 (aa); 75 (kD predicted)

(Rosenthal et al., 1987)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pkc53E using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain

(Schaeffer et al., 1989)

(Rosenthal et al., 1987)

retina

(Schaeffer et al., 1989)

adult head

(Schaeffer et al., 1989)

Additional Descriptive Data

Pkc53E transcripts are detected in all head neural tissue by in situ hybridization. They are observed in RNA from adult head but not adult body on northern blots.

(Schaeffer et al., 1989)

Pkc53E transcripts are detected in adult RNA but not in RNA from 0-3hr embryos.

(Rosenthal et al., 1987)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: Mi{Trojan-GAL4.0}Pkc53E^{MI05296-TG4.0}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | restricted

(Lee et al., 2018)

adult mushroom body

(Lee et al., 2018)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Pkc53E in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pkc53E

View exonic expression by tissue for Dmel\Pkc53E

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

48311/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003091

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003091

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003091

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6622

Flygut - An atlas of the Drosophila adult midgut

FBgn0003091

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pkc53E

Transgenic constructs containing regulatory region of Pkc53E

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2R)Exel6065

(Ryder, 2004.4.26)

Gene partially disrupted in

Df(2R)CG15614^attP

(Rao and Deng, 2019.10.23)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Pkc53E^EY14093

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pkc53E^GD11984

viable, with Scer\GAL4^Mef2.PR

Pkc53E^GD11984

viable, with Scer\GAL4^pnr-MD237

Pkc53E^GD11984

viable, with Scer\GAL4^tin.CΔ4

Pkc53E^GD11984

Sterility

Allele

fertile

Pkc53E^EY14093

Other Phenotypes

Allele

abnormal size | adult stage | dominant

Pkc53E^EY14093

visible, with Scer\GAL4^Act88F.PD

Pkc53E^kin.UAS

visible, with Scer\GAL4^Cy6

Pkc53E^kin.UAS

Phenotype manifest in

Allele

wing blade

Pkc53E^EY14093

wing margin, with Scer\GAL4^Cy6

Pkc53E^kin.UAS

wing vein | ectopic

Pkc53E^EY14093

wing vein | ectopic, with Scer\GAL4^Act88F.PD

Pkc53E^kin.UAS

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (20)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PRKCA

15 of 15

Yes

Hsap\PRKCB

13 of 15

Yes

Hsap\PRKCG

10 of 15

Yes

3 of 15

3 of 15

3 of 15

3 of 15

2 of 15

2 of 15

2 of 15

2 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (19)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Prkca

15 of 15

Yes

Mmus\Prkcb

13 of 15

Yes

Mmus\Prkcg

11 of 15

Yes

3 of 15

3 of 15

3 of 15

3 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (19)

Rnor\Prkca

12 of 13

Yes

Rnor\Prkcb

11 of 13

Yes

Rnor\Prkcg

8 of 13

Yes

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (54)

Xtro\prkcb

10 of 12

Yes

Xtro\prkca

8 of 12

Yes

Xtro\prkcg

5 of 12

Yes

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

Yes

Xtro\gpha2

1 of 12

Xtro\LOC100485620

1 of 12

Yes

Xtro\LOC100488597

1 of 12

Yes

Xtro\LOC100488761

1 of 12

Yes

Xtro\LOC100488890

1 of 12

Yes

Xtro\LOC100491734

1 of 12

Xtro\LOC100492960

1 of 12

Yes

Xtro\LOC100494592

1 of 12

Yes

Xtro\LOC100495168

1 of 12

Yes

Xtro\LOC100496120

1 of 12

Yes

Xtro\LOC100498008

1 of 12

Yes

Xtro\LOC100498287

1 of 12

Yes

Xtro\LOC101730416

1 of 12

Yes

Xtro\LOC101731335

1 of 12

Yes

Xtro\LOC101731405

1 of 12

Yes

Xtro\LOC101731784

1 of 12

Yes

Xtro\LOC101731822

1 of 12

Yes

Xtro\LOC101732209

1 of 12

Yes

Xtro\LOC101732266

1 of 12

Yes

Xtro\LOC101732325

1 of 12

Yes

Xtro\LOC101732362

1 of 12

Yes

Xtro\LOC101732451

1 of 12

Yes

Xtro\LOC101732692

1 of 12

Yes

Xtro\LOC101733271

1 of 12

Yes

Xtro\LOC101733758

1 of 12

Yes

Xtro\LOC101733770

1 of 12

Yes

Xtro\LOC101733833

1 of 12

Yes

Xtro\LOC101733940

1 of 12

Yes

Xtro\LOC101734218

1 of 12

Yes

Xtro\LOC101734329

1 of 12

Yes

Xtro\LOC105948370

1 of 12

Yes

Xtro\LOC105948372

1 of 12

Yes

Xtro\LOC108645367

1 of 12

Yes

Xtro\LOC108648806

1 of 12

Yes

Xtro\LOC108648813

1 of 12

Yes

Xtro\LOC108648814

1 of 12

Yes

Xtro\pkn1

1 of 12

Xtro\pkn3

1 of 12

Xtro\tnfrsf1a

1 of 12

Danio rerio (Zebrafish) (30)

Drer\prkcbb

13 of 15

Yes

Drer\prkcaa

12 of 15

Yes

Drer\prkcba

12 of 15

Yes

Drer\prkcg

8 of 15

Yes

Drer\si:ch73-374l24.1

6 of 15

Yes

3 of 15

3 of 15

3 of 15

3 of 15

3 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

Yes

Drer\sgk3

2 of 15

Drer\si:ch211-195b13.1

2 of 15

Yes

Drer\zgc:153916

2 of 15

Drer\pkn1b

1 of 15

Drer\pkn2

1 of 15

Drer\si:dkey-121j17.5

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (10)

Cele\pkc-2

14 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (20)

Atha\AGC1.5

1 of 9

Yes

Atha\AGC1.7

1 of 9

Yes

Atha\AT1G17540

1 of 9

Yes

Atha\AT2G44830

1 of 9

Yes

Atha\AT3G44610

1 of 9

Yes

Atha\AT4G10010

1 of 9

Yes

Atha\AT5G40030

1 of 9

Yes

Atha\ATPK7

1 of 9

Yes

Atha\CERK1

1 of 9

Yes

Atha\CRLK2

1 of 9

Yes

Atha\D6PK

1 of 9

Yes

Atha\D6PKL1

1 of 9

Yes

Atha\D6PKL2

1 of 9

Yes

Atha\KIPK

1 of 9

Yes

Atha\PID2

1 of 9

Yes

Atha\PID

1 of 9

Yes

Atha\RSH3

1 of 9

Yes

Atha\SWAP70

1 of 9

Yes

Atha\WAG1

1 of 9

Yes

Atha\WAG2

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\PKC1

6 of 15

Yes

Scer\SCH9

1 of 15

Scer\YPK1

1 of 15

Scer\YPK2

1 of 15

Schizosaccharomyces pombe (Fission yeast) (6)

Spom\pck1

6 of 12

Yes

Spom\pck2

6 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (13)

Gene Symbol

Score

Source

Alignment

7 of 10

6 of 10

5 of 10

5 of 10

Akt

4 of 10

Pkn

3 of 10

CG32944

1 of 10

Dgk

1 of 10

dop

1 of 10

gwl

1 of 10

Pdk1

1 of 10

trc

1 of 10

wts

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Pkc53E^GD11984

DOES NOT model alcohol use disorder

CEA

(Chen et al., 2008)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

PRKCG; protein kinase C gamma

model of spinocerebellar ataxia type 14

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Pkc53E^GD11984

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-231.UAS}

(Onur et al., 2021)

Pkc53E^HMC04134

exacerbates amyotrophic lateral sclerosis type 10

modeled by TBPH^{UAS.Tag:FLAG.Tag:HA}

(Park et al., 2020)

Pkc53E^EY14093

exacerbates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PRKCA; protein kinase C alpha

15 of 15

176960

PRKCB; protein kinase C beta

13 of 15

176970

PRKCG; protein kinase C gamma

10 of 15

176980

SPINOCEREBELLAR ATAXIA 14; SCA14

spinocerebellar ataxia type 14

PRKCE; protein kinase C epsilon

3 of 15

176975

PRKCH; protein kinase C eta

3 of 15

605437

STROKE, ISCHEMIC

cerebral infarction

PRKCI; protein kinase C iota

3 of 15

600539

PRKCZ; protein kinase C zeta

3 of 15

176982

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Pkc53E - IKKε

anti tag coimmunoprecipitation, anti tag western blot

(Otani et al., 2016)

Pkc53E - gcm

anti tag coimmunoprecipitation, anti tag western blot

(Xi et al., 2017)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

Pkc53E

(Murillo-Maldonado et al., 2011)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0003091

MIST (protein-protein) - An integrated Molecular Interaction Database

Pkc53E

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0003091

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-81

Cytogenetic map

53E2-53E2

Sequence location

2R:16,924,655..16,945,126 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

53E2-53E2

Limits computationally determined from genome sequence between P{lacW}CG6426^k10209&P{lacW}veg^k07202 and P{lacW}l(2)k07408^k07408

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

53E4-53E7

(Schaeffer et al., 1989)

53E-53E

(determined by in situ hybridisation)

(Rosenthal et al., 1987)

Experimentally Determined Recombination Data

Location

2-81

(FlyBase, 2016)

2-84.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (16)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

80988

w¹¹¹⁸; Pkc53E^Δ28

24443

w¹¹¹⁸; Mi{ET1}Pkc53E^MB02781

27491

y¹ v¹; P{TRiP.JF02641}attP2

42309

y¹ w^*; Mi{MIC}Pkc53E^MI05296

20790

y¹ w^67c23; P{EPgy2}Pkc53E^EY14093/CyO

84470

w^*; Df(2R)CG15614^attP, TI{TI}CG15614^attP

80989

w^*; Pkc53E^Δ28; P{UAS-Pkc53E.RB}3/TM6B, Tb¹

67462

y¹ w^*; Mi{Trojan-GAL4.0}Pkc53E^{MI05296-TG4.0}

59413

y¹ w^67c23; Mi{PT-GFSTF.0}Pkc53E^{MI05296-GFSTF.0}

VDRC

v27699

P{GD11984}v27699

v27696

w¹¹¹⁸; P{GD11984}v27696/TM3

v341127

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00147}attP40/CyO-GFP

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Genomic Clones (28)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (10)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH03188

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003091

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003091

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

48311

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Pkc53E CG6622

(Bayraktaroglu, 2000.4.13)

Additional comments

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

The effect of Pkc53E mediated phosphorylation on each step of Top2 catalytic cycle is analysed. As with Casein kinase II (CkIIβ and CkIIβ), stimulation of enzyme activity by Pkc53E correlates with an increased rate of Top2 catalysed ATP hydrolysis. Modification did not affect any step of the enzymes catalytic cycle that preceded the ATPase step.

(Corbett et al., 1993)

Activation of endogenous Pka-C1, expression of wild type Tl receptors or treatment of cells with activators of Pkc53E and radical oxygen intermediates have only a marginal effects on the cellular distribution of dl protein in culture cells.

(Kubota et al., 1993)

Comparison of CpG distribution in the coding region of 121 genes from six species supports the mCpG mutational hotspot explanation of CpG suppression in methylated species at position II-III and III-I.

(Schorderet and Gartler, 1992)

inaC maps within 50kb of Pkc53E.

(Schaeffer et al., 1989)

Isolated by homology to bovine protein kinase C.

(Rosenthal et al., 1987)

Pkc53E has been cloned and characterised.

(Rosenthal et al., 1987)

The protein kinase C (PKC) enzyme in Drosophila is encoded by a gene family made up of three genes which are expressed primarily in adult flies. The deduced amino acid sequences of these genes are quite similar to each other and those of the mammalian PKC family (Schaeffer, Smith, Mardon, Quinn and Zuker, 1989). Ca⁺⁺-dependent Molecular data indicate that Pkc53E lies approximately 20 kb from inaC, another PKC gene with 53E cytological location (Schaeffer, Smith, Mardon, Quinn and Zuker, 1989).

Structural gene for protein kinase C (PKC) that is expressed primarily in the brain of adult flies.

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 95 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

48311

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P05130

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P05130

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P05130

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003091

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

48311/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003091

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003091

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003091

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6622

Flygut - An atlas of the Drosophila adult midgut

FBgn0003091

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0003091

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

48311

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG6622

MARRVEL_MODEL - MARRVEL (model organism gene)

48311

modMine - A data warehouse for the modENCODE project

FBgn0003091

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P05130

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0003091

DRSC - Results frm RNAi screens

FBgn0003091

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003091

FlyMine - An integrated database for Drosophila genomics

FBgn0003091

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (protein-protein) - An integrated Molecular Interaction Database

Pkc53E

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (23)

Reported As

Symbol Synonym

CG6622

(Kanamori et al., 2013, Ni et al., 2010.12.1, Perkins et al., 2009, Morrison et al., 2000)

DPKC

(Llamazares et al., 1991)

Dpkc1

(Franke et al., 1994)

PK-C

(Brubaker-Purkey and Woodruff, 2013)

PKC

(Voolstra and Huber, 2020, Getahun et al., 2013, Sun et al., 2007, Roderick and Bootman, 2003, Voets and Nilius, 2003, Pandur et al., 2002, Hardie and Raghu, 2001, Hoyos et al., 2000, Broughton et al., 1995, Kubota et al., 1993, Schorderet and Gartler, 1992, Rosenthal, 1987.5.8, Rosenthal, 1987.5.8)

PKC 53B

(Morrison et al., 2000)

PKC 53E

(Wodarz et al., 2000)

PKC-53B

(Bjorklund et al., 2006)

PKC-53E

(Bjorklund et al., 2006)

PKC1

(Lee et al., 2015, Smith et al., 1991)

PKC53E

(Poudel et al., 2021, Chvilicek et al., 2020, Li et al., 2020, Maier et al., 2019, Xi et al., 2017, Getahun et al., 2016, Otani et al., 2016, Wang et al., 2012, Murillo-Maldonado et al., 2011, Chen et al., 2010, Chen et al., 2008, Betson and Settleman, 2007, Shieh et al., 2002)

PKC53e

(Colomb and Brembs, 2016)

Pkc1

(Lindsley and Zimm, 1992)