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FB2021_03, released June 15, 2021

Gene: Dmel\Pkc53E

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General Information
Symbol
Dmel\Pkc53E
Species
D. melanogaster
Name
Protein C kinase 53E
Annotation Symbol
CG6622
Feature Type
protein_coding_gene
FlyBase ID
FBgn0003091
Gene Model Status
Current
Stock Availability
16 publicly available
Enzyme Name (EC)
Protein kinase C (2.7.11.13)
Dual-specificity kinase (2.7.12.1)
Gene Summary
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins (By similarity). PKC also serves as the receptor for phorbol esters, a class of tumor promoters (By similarity). Acts in a hh-signaling pathway which regulates the Duox-dependent gut immune response to bacterial uracil; required for the activation of Cad99C and consequently Cad99C-dependent endosome formation, which is essential for the Duox-dependent production of reactive oxygen species (ROS) in response to intestinal bacterial infection (PubMed:25639794). (UniProt, P05130)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

PKC, protein kinase C, dPKC, dPKC53E(br), Pkc1

Key Links
Genomic Location
Cytogenetic map
53E2-53E2
Sequence location
2R:16,924,655..16,945,126 [+]
Recombination map
2-81
RefSeq locus
NT_033778 REGION:16924655..16945126
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (11 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables protein serine/threonine kinase activity
inferred from direct assay
(Corbett et al., 1993)
enables protein serine/threonine/tyrosine kinase activity
inferred from mutant phenotype
(Xi et al., 2017)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables ATP binding
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables protein kinase C activity
inferred from electronic annotation with InterPro:IPR014375
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
inferred from sequence or structural similarity
(Rosenthal et al., 1987)
enables protein serine/threonine kinase activity
inferred from biological aspect of ancestor with PANTHER:PTN000683254
(assigned by GO_Central )
(Gaudet et al., 2011)
enables zinc ion binding
inferred from electronic annotation with InterPro:IPR014375
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Biological Process (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in positive regulation of clathrin-dependent endocytosis
inferred from genetic interaction with FLYBASE:inaC; FB:FBgn0004784
(Lee et al., 2015)
involved_in protein phosphorylation
inferred from direct assay
(Corbett et al., 1993)
involved_in regulation of hemocyte proliferation
inferred from mutant phenotype
(Sinenko et al., 2010)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in intracellular signal transduction
inferred from biological aspect of ancestor with PANTHER:PTN000683254
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in peptidyl-serine phosphorylation
inferred from biological aspect of ancestor with PANTHER:PTN000683254
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in plasma membrane
inferred from sequence or structural similarity with FLYBASE:inaC; FB:FBgn0004784
(FlyBase Curators, 2008--)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. (P05130)
Protein Signatures (InterPro)
Catalytic Activity (EC)
Experimental Evidence
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L- seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein] (2.7.12.1)
Predictions / Assertions
ATP + a protein = ADP + a phosphoprotein (2.7.11.13)
Summaries
Gene Group (FlyBase)
PROTEIN KINASE C FAMILY -
The Protein Kinase C (PKC) family are serine/threonine kinases activated by diacylglycerol (DAG), lipid or Ca2+. PKCs are regulated by auto-inhibition through a pseudosubstrate site located in the regulatory domain. (Adapted from PMID:20094051).
Protein Function (UniProtKB)
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins (By similarity). PKC also serves as the receptor for phorbol esters, a class of tumor promoters (By similarity). Acts in a hh-signaling pathway which regulates the Duox-dependent gut immune response to bacterial uracil; required for the activation of Cad99C and consequently Cad99C-dependent endosome formation, which is essential for the Duox-dependent production of reactive oxygen species (ROS) in response to intestinal bacterial infection (PubMed:25639794).
(UniProt, P05130)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Pkc1: Protein kinase-c1
Structural gene for protein kinase C (PKC) that is expressed primarily in the brain of adult flies.
Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
4

Please see the JBrowse view of Dmel\Pkc53E for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Pkc53E-RA
FBtr0087049
NM_057334
3175
670
Pkc53E-RB
FBtr0087048
NM_166201
2696
679
Pkc53E-RC
FBtr0112819
NM_001103880
2693
678
Pkc53E-RD
FBtr0305069
NM_001259448
2024
525
Pkc53E-RE
FBtr0305070
NM_001259449
2633
678
Pkc53E-RF
FBtr0331960
NM_001274106
4711
670
Additional Transcript Data and Comments
Reported size (kB)

4.3, 4.0, 2.4 (northern blot)

(Rosenthal et al., 1987)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Pkc53E-PA
FBpp0086198
76.7
670
7.15
NP_476682
AAF57932
Pkc53E-PB
FBpp0086197
77.7
679
7.14
NP_725626
AAF57933
Pkc53E-PC
FBpp0111731
77.7
678
7.67
NP_001097350
ABV53829
Pkc53E-PD
FBpp0293606
60.4
525
6.27
NP_001246377
AFH08130
Pkc53E-PE
FBpp0293607
77.7
678
7.67
NP_001246378
AFH08131
Pkc53E-PF
FBpp0304291
76.7
670
7.15
NP_001261035
AGB93567
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

670 aa isoforms: Pkc53E-PA, Pkc53E-PF
678 aa isoforms: Pkc53E-PC, Pkc53E-PE
Additional Polypeptide Data and Comments
Reported size (kDa)

670 (aa)

(Schaeffer, 1998.11.23)

639 (aa); 75 (kD predicted)

(Rosenthal et al., 1987)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pkc53E using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult brain
(Schaeffer et al., 1989)
(Rosenthal et al., 1987)
retina
(Schaeffer et al., 1989)
adult head
(Schaeffer et al., 1989)
Additional Descriptive Data

Pkc53E transcripts are detected in all head neural tissue by in situ hybridization. They are observed in RNA from adult head but not adult body on northern blots.

(Schaeffer et al., 1989)

Pkc53E transcripts are detected in adult RNA but not in RNA from 0-3hr embryos.

(Rosenthal et al., 1987)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
Reporter: Mi{Trojan-GAL4.0}Pkc53EMI05296-TG4.0
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult brain | restricted
(Lee et al., 2018)
adult mushroom body
(Lee et al., 2018)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Pkc53E in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB
EMBL-EBI Single Cell Expression Atlas
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 8 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Pkc53E
Transgenic constructs containing regulatory region of Pkc53E
Aberrations (Deficiencies and Duplications) ( 2 )
Inferred from experimentation ( 2 )
Gene disrupted in
Df(2R)Exel6065
(Ryder, 2004.4.26)
Gene partially disrupted in
Df(2R)CG15614attP
(Rao and Deng, 2019.10.23)
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (20)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\PRKCA
15 of 15
Yes
Yes
Hsap\PRKCB
13 of 15
No
Yes
1  
Hsap\PRKCG
10 of 15
No
Yes
Hsap\PRKCE
3 of 15
No
No
Hsap\PRKCH
3 of 15
No
No
Hsap\PRKCI
3 of 15
No
No
Hsap\PRKCZ
3 of 15
No
No
Hsap\AKT1
2 of 15
No
No
1  
Hsap\AKT2
2 of 15
No
No
5  
Hsap\AKT3
2 of 15
No
No
Hsap\C8orf44-SGK3
2 of 15
No
Yes
Hsap\PKN1
2 of 15
No
No
Hsap\PKN2
2 of 15
No
No
Hsap\PKN3
2 of 15
No
No
Hsap\PRKCD
2 of 15
No
No
Hsap\PRKCQ
2 of 15
No
No
Hsap\SGK1
2 of 15
No
No
Hsap\SGK2
2 of 15
No
No
Hsap\DOC2B
1 of 15
No
No
Hsap\SGK3
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (19)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Prkca
15 of 15
Yes
Yes
Mmus\Prkcb
13 of 15
No
Yes
Mmus\Prkcg
11 of 15
No
Yes
Mmus\Prkce
3 of 15
No
No
Mmus\Prkch
3 of 15
No
No
Mmus\Prkci
3 of 15
No
No
Mmus\Prkcz
3 of 15
No
No
3  
Mmus\Akt1
2 of 15
No
No
Mmus\Akt2
2 of 15
No
No
Mmus\Pkn1
2 of 15
No
No
Mmus\Pkn2
2 of 15
No
No
Mmus\Pkn3
2 of 15
No
No
Mmus\Prkcd
2 of 15
No
No
Mmus\Prkcq
2 of 15
No
No
Mmus\Sgk1
2 of 15
No
No
Mmus\Sgk2
2 of 15
No
No
Mmus\Sgk3
2 of 15
No
No
Mmus\Akt3
1 of 15
No
No
Mmus\Doc2b
1 of 15
No
No
Rattus norvegicus (Norway rat) (19)
Rnor\Prkca
12 of 13
Yes
Yes
Rnor\Prkcb
11 of 13
No
Yes
Rnor\Prkcg
8 of 13
No
Yes
Rnor\Prkci
3 of 13
No
No
Rnor\Prkcz
3 of 13
No
No
Rnor\Akt1
2 of 13
No
No
Rnor\Akt2
2 of 13
No
No
Rnor\Akt3
2 of 13
No
No
Rnor\Prkch
2 of 13
No
No
Rnor\Sgk1
2 of 13
No
No
Rnor\Sgk2
2 of 13
No
No
Rnor\Sgk3
2 of 13
No
Yes
Rnor\Doc2b
1 of 13
No
No
Rnor\Pkn1
1 of 13
No
No
Rnor\Pkn2
1 of 13
No
No
Rnor\Pkn3
1 of 13
No
No
Rnor\Prkcd
1 of 13
No
No
Rnor\Prkce
1 of 13
No
No
Rnor\Prkcq
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (54)
Xtro\prkcb
10 of 12
Yes
Yes
Xtro\prkca
8 of 12
No
Yes
Xtro\prkcg
5 of 12
No
Yes
Xtro\prkce
3 of 12
No
No
Xtro\prkci
3 of 12
No
No
Xtro\prkcz
3 of 12
No
No
Xtro\akt1
2 of 12
No
No
Xtro\akt2
2 of 12
No
No
Xtro\LOC100493742
2 of 12
No
No
Xtro\prkcd
2 of 12
No
No
Xtro\prkch
2 of 12
No
No
Xtro\prkcq
2 of 12
No
No
Xtro\sgk1
2 of 12
No
No
Xtro\sgk2
2 of 12
No
No
Xtro\sgk3
2 of 12
No
Yes
Xtro\gpha2
1 of 12
No
No
Xtro\LOC100485620
1 of 12
No
Yes
Xtro\LOC100488597
1 of 12
No
Yes
Xtro\LOC100488761
1 of 12
No
Yes
Xtro\LOC100488890
1 of 12
No
Yes
Xtro\LOC100491734
1 of 12
No
No
Xtro\LOC100492960
1 of 12
No
Yes
Xtro\LOC100494592
1 of 12
No
Yes
Xtro\LOC100495168
1 of 12
No
Yes
Xtro\LOC100496120
1 of 12
No
Yes
Xtro\LOC100498008
1 of 12
No
Yes
Xtro\LOC100498287
1 of 12
No
Yes
Xtro\LOC101730416
1 of 12
No
Yes
Xtro\LOC101731335
1 of 12
No
Yes
Xtro\LOC101731405
1 of 12
No
Yes
Xtro\LOC101731784
1 of 12
No
Yes
Xtro\LOC101731822
1 of 12
No
Yes
Xtro\LOC101732209
1 of 12
No
Yes
Xtro\LOC101732266
1 of 12
No
Yes
Xtro\LOC101732325
1 of 12
No
Yes
Xtro\LOC101732362
1 of 12
No
Yes
Xtro\LOC101732451
1 of 12
No
Yes
Xtro\LOC101732692
1 of 12
No
Yes
Xtro\LOC101733271
1 of 12
No
Yes
Xtro\LOC101733758
1 of 12
No
Yes
Xtro\LOC101733770
1 of 12
No
Yes
Xtro\LOC101733833
1 of 12
No
Yes
Xtro\LOC101733940
1 of 12
No
Yes
Xtro\LOC101734218
1 of 12
No
Yes
Xtro\LOC101734329
1 of 12
No
Yes
Xtro\LOC105948370
1 of 12
No
Yes
Xtro\LOC105948372
1 of 12
No
Yes
Xtro\LOC108645367
1 of 12
No
Yes
Xtro\LOC108648806
1 of 12
No
Yes
Xtro\LOC108648813
1 of 12
No
Yes
Xtro\LOC108648814
1 of 12
No
Yes
Xtro\pkn1
1 of 12
No
No
Xtro\pkn3
1 of 12
No
No
Xtro\tnfrsf1a
1 of 12
No
No
Danio rerio (Zebrafish) (30)
Drer\prkcbb
13 of 15
Yes
Yes
Drer\prkcaa
12 of 15
No
Yes
Drer\prkcba
12 of 15
No
Yes
Drer\prkcg
8 of 15
No
Yes
Drer\si:ch73-374l24.1
6 of 15
No
Yes
Drer\prkcea
3 of 15
No
No
Drer\prkceb
3 of 15
No
No
Drer\prkcha
3 of 15
No
No
Drer\prkci
3 of 15
No
No
Drer\prkcz
3 of 15
No
No
Drer\akt1
2 of 15
No
No
Drer\akt2
2 of 15
No
No
Drer\akt2l
2 of 15
No
No
Drer\akt3a
2 of 15
No
No
Drer\akt3b
2 of 15
No
No
Drer\pkn1a
2 of 15
No
No
Drer\pkn3
2 of 15
No
No
Drer\prkcda
2 of 15
No
No
Drer\prkcdb
2 of 15
No
No
Drer\prkchb
2 of 15
No
No
Drer\prkcq
2 of 15
No
No
Drer\sgk1
2 of 15
No
No
Drer\sgk2a
2 of 15
No
No
Drer\sgk2b
2 of 15
No
Yes
Drer\sgk3
2 of 15
No
No
Drer\si:ch211-195b13.1
2 of 15
No
Yes
Drer\zgc:153916
2 of 15
No
No
Drer\pkn1b
1 of 15
No
No
Drer\pkn2
1 of 15
No
No
Drer\si:dkey-121j17.5
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (10)
Cele\pkc-2
14 of 15
Yes
Yes
Cele\akt-1
2 of 15
No
No
Cele\akt-2
2 of 15
No
No
Cele\pkc-1
2 of 15
No
No
Cele\pkc-3
2 of 15
No
No
Cele\sgk-1
2 of 15
No
No
Cele\tpa-1
2 of 15
No
No
Cele\C56G2.5
1 of 15
No
No
Cele\F31E3.2
1 of 15
No
No
Cele\pkn-1
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (20)
Atha\AGC1.5
1 of 9
Yes
Yes
Atha\AGC1.7
1 of 9
Yes
Yes
Atha\AT1G17540
1 of 9
Yes
Yes
Atha\AT2G44830
1 of 9
Yes
Yes
Atha\AT3G44610
1 of 9
Yes
Yes
Atha\AT4G10010
1 of 9
Yes
No
Atha\AT5G40030
1 of 9
Yes
Yes
Atha\ATPK7
1 of 9
Yes
Yes
Atha\CERK1
1 of 9
Yes
Yes
Atha\CRLK2
1 of 9
Yes
Yes
Atha\D6PK
1 of 9
Yes
Yes
Atha\D6PKL1
1 of 9
Yes
Yes
Atha\D6PKL2
1 of 9
Yes
Yes
Atha\KIPK
1 of 9
Yes
Yes
Atha\PID2
1 of 9
Yes
Yes
Atha\PID
1 of 9
Yes
Yes
Atha\RSH3
1 of 9
Yes
Yes
Atha\SWAP70
1 of 9
Yes
Yes
Atha\WAG1
1 of 9
Yes
Yes
Atha\WAG2
1 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (4)
Scer\PKC1
6 of 15
Yes
No
Scer\SCH9
1 of 15
No
No
Scer\YPK1
1 of 15
No
No
Scer\YPK2
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (6)
Spom\pck1
6 of 12
Yes
No
Spom\pck2
6 of 12
Yes
No
Spom\gad8
1 of 12
No
No
Spom\psk1
1 of 12
No
No
Spom\sck1
1 of 12
No
No
Spom\sck2
1 of 12
No
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091903HV )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila simulans
Dsim\GD11233
Drosophila sechellia
Dsec\GM21740
Drosophila erecta
Dere\GG20645
Drosophila yakuba
Dyak\GE11630
Drosophila ananassae
Dana\GF11424
Drosophila pseudoobscura pseudoobscura
Dpse\GA19732
Drosophila persimilis
Dper\GL11571
Drosophila willistoni
Dwil\GK22032
Drosophila virilis
Dvir\GJ21016
Drosophila mojavensis
Dmoj\GI21163
Drosophila grimshawi
Dgri\GH22650
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915024Q )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17019857
Musca domestica
House fly
Mdoa\17019858
Musca domestica
House fly
Mdoa\17020029
Glossina morsitans
Tsetse fly
Gmor\GMOY012181
Glossina morsitans
Tsetse fly
Gmor\GMOY004136
Glossina morsitans
Tsetse fly
Gmor\GMOY004974
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_09853
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_08495
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_09856
Mayetiola destructor
Hessian fly
Mdes\Mdes009305
Mayetiola destructor
Hessian fly
Mdes\Mdes007044
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL001108
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL000810
Anopheles darlingi
American malaria mosquito
Adar\ADAC005513
Anopheles darlingi
American malaria mosquito
Adar\ADAC002326
Anopheles gambiae
Malaria mosquito
Agam\AGAP012252
Anopheles gambiae
Malaria mosquito
Agam\AGAP002748
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ013331
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ005701
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ020037
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00TF )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA012362
Bombyx mori
Silkmoth
Bmor\BGIBMGA014132
Bombyx mori
Silkmoth
Bmor\BGIBMGA000779
Bombyx mori
Silkmoth
Bmor\BGIBMGA014073
Danaus plexippus
Monarch butterfly
Dple\DPOGS206317-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS207594-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS212831-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL012606
Heliconius melpomene
Postman butterfly
Hmel\HMEL012605
Heliconius melpomene
Postman butterfly
Hmel\HMEL004485
Heliconius melpomene
Postman butterfly
Hmel\HMEL009727
Apis florea
Little honeybee
Aflo\4805631172
Apis florea
Little honeybee
Aflo\2157451284
Apis florea
Little honeybee
Aflo\2163578740
Apis florea
Little honeybee
Aflo\2249420180
Apis mellifera
Western honey bee
Amel\GB43222
Apis mellifera
Western honey bee
Amel\GB41861
Apis mellifera
Western honey bee
Amel\GB41862
Apis mellifera
Western honey bee
Amel\GB42064
Apis mellifera
Western honey bee
Amel\GB42063
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP10189
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22891
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22539
Bombus terrestris
Buff-tailed bumblebee
Bter\15210488
Bombus terrestris
Buff-tailed bumblebee
Bter\15216357
Bombus terrestris
Buff-tailed bumblebee
Bter\15208091
Linepithema humile
Argentine ant
Lhum\LH11551
Linepithema humile
Argentine ant
Lhum\LH20663
Linepithema humile
Argentine ant
Lhum\LH11001
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000910
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0000b0
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001d7c
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00090f
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG013022
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG004720
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG004722
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG009095
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_07320
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_12645
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_01637
Tribolium castaneum
Red flour beetle
Tcas\TC008637
Tribolium castaneum
Red flour beetle
Tcas\TC032364
Tribolium castaneum
Red flour beetle
Tcas\TC008638
Tribolium castaneum
Red flour beetle
Tcas\TC032365
Pediculus humanus
Human body louse
Phum\PHUM544690
Pediculus humanus
Human body louse
Phum\PHUM528520
Pediculus humanus
Human body louse
Phum\PHUM513980
Rhodnius prolixus
Kissing bug
Rpro\RPRC003862
Rhodnius prolixus
Kissing bug
Rpro\RPRC009257
Rhodnius prolixus
Kissing bug
Rpro\RPRC015071
Cimex lectularius
Bed bug
Clec\CLEC012459
Cimex lectularius
Bed bug
Clec\CLEC004010
Cimex lectularius
Bed bug
Clec\CLEC005935
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI002147
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI082181
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI002684
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_08853
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_13422
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_08855
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_09104
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01FN )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR009568
Strigamia maritima
European centipede
Smar\SMAR013053
Strigamia maritima
European centipede
Smar\SMAR005954
Strigamia maritima
European centipede
Smar\SMAR015359
Ixodes scapularis
Black-legged tick
Isca\ISCW004410
Ixodes scapularis
Black-legged tick
Isca\ISCW020000
Ixodes scapularis
Black-legged tick
Isca\ISCW007464
Ixodes scapularis
Black-legged tick
Isca\ISCW023594
Stegodyphus mimosarum
African social velvet spider
Smim\KFM72002.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM72001.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM73764.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM78941.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM82440.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM58070.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur16g01090
Tetranychus urticae
Two-spotted spider mite
Turt\tetur03g03970
Tetranychus urticae
Two-spotted spider mite
Turt\tetur03g06180
Tetranychus urticae
Two-spotted spider mite
Turt\tetur33g01220
Tetranychus urticae
Two-spotted spider mite
Turt\tetur18g02560
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_301963
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_309380
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_313594
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_326383
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_237284
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_319272
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0QRS )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Hypp_2253
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Gpr101L_2
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Hypp_873
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Gpr101L_5
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Hypp_2387
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Pkcd
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Pkce_1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Hypp_1688
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_008428gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Hypp_17
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_013182gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Prg4
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Pkc1_2
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Gpr101L_6
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Hypp_721
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Gpr101L_7
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Pkc1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Hypp_2786
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Pkce
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Hypp_2463
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_021522gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Hypp_452
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Gpr101L_1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Gpr101L_3
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000018761
Ciona intestinalis
Vase tunicate
Cint\LOC100186768
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000023077
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000004473
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000024732
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000024503
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000025950
Gallus gallus
Domestic chicken
Ggal\PRKCD
Gallus gallus
Domestic chicken
Ggal\PRKCA
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000011656
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000028330
Gallus gallus
Domestic chicken
Ggal\PRKCB
Gallus gallus
Domestic chicken
Ggal\PRKCH
Gallus gallus
Domestic chicken
Ggal\Gga.10658
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000023876
Gallus gallus
Domestic chicken
Ggal\PRKCE
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (13)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
inaC
7 of 10
Pkcδ
6 of 10
aPKC
5 of 10
Pkc98E
5 of 10
Akt
4 of 10
Pkn
3 of 10
CG32944
1 of 10
Dgk
1 of 10
dop
1 of 10
gwl
1 of 10
Pdk1
1 of 10
trc
1 of 10
wts
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Evidence
    References
    Pkc53EGD11984
    DOES NOT model  alcohol use disorder
    CEA
    (Chen et al., 2008)
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    PRKCG; protein kinase C gamma
    model of  spinocerebellar ataxia type 14
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    Pkc53EEY14093
    exacerbates  acute myeloid leukemia
    modeled by Hsap\RUNX1::Hsap\RUNX1T1UAS.cSa
    (Sinenko et al., 2010)
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    PRKCA; protein kinase C alpha
    15 of 15
    176960
        PRKCB; protein kinase C beta
        13 of 15
        176970
             
            PRKCG; protein kinase C gamma
            10 of 15
            176980
            PRKCE; protein kinase C epsilon
            3 of 15
            176975
                PRKCH; protein kinase C eta
                3 of 15
                605437
                PRKCI; protein kinase C iota
                3 of 15
                600539
                    PRKCZ; protein kinase C zeta
                    3 of 15
                    176982
                        Functional Complementation Data
                        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                        Interactions
                        Summary of Physical Interactions
                        esyN Network Diagram
                        Show neighbor-neighbor interactions:
                        Select Layout:
                        Legend:
                        Protein
                        RNA
                        Selected Interactor(s)
                        Interactions Browser
                        View in Interactions Browser
                        Please see the Physical Interaction reports below for full details
                        protein-protein
                        Physical Interaction
                        Assay
                        References
                        Pkc53E - IKKε
                        anti tag coimmunoprecipitation, anti tag western blot
                        (Otani et al., 2016)
                        Pkc53E - gcm
                        anti tag coimmunoprecipitation, anti tag western blot
                        (Xi et al., 2017)
                        Summary of Genetic Interactions
                        esyN Network Diagram
                        esyN Network Key:
                        Suppression
                        Enhancement
                        View in Interactions Browser
                        Please look at the allele data for full details of the genetic interactions
                        Starting gene(s)
                        Interaction type
                        Interacting gene(s)
                        Reference
                        Starting gene(s)
                        Interaction type
                        Interacting gene(s)
                        Reference
                        sl
                        enhanceable
                        Pkc53E
                        (Murillo-Maldonado et al., 2011)
                        External Data
                        Linkouts
                        DroID - A comprehensive database of gene and protein interactions.
                        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                        MIST (protein-protein) - An integrated Molecular Interaction Database
                        Pathways
                        Genomic Location and Detailed Mapping Data
                        Chromosome (arm)
                        2R
                        Recombination map
                        2-81
                        Cytogenetic map
                        53E2-53E2
                        Sequence location
                        2R:16,924,655..16,945,126 [+]
                        FlyBase Computed Cytological Location
                        Cytogenetic map
                        Evidence for location
                        53E2-53E2
                        Limits computationally determined from genome sequence between P{lacW}CG6426k10209&P{lacW}vegk07202 and P{lacW}l(2)k07408k07408
                        Experimentally Determined Cytological Location
                        Cytogenetic map
                        Notes
                        References
                        53E4-53E7
                        (Schaeffer et al., 1989)
                        53E-53E
                        (determined by in situ hybridisation)
                        (Rosenthal et al., 1987)
                        Experimentally Determined Recombination Data
                        Location

                        2-81

                        (FlyBase, 2016)

                        2-84.8

                        (Comeron et al., 2012)
                        Left of (cM)
                        Right of (cM)
                        Notes
                        Stocks and Reagents
                        Stocks (16)
                        Genomic Clones (28)
                        cDNA Clones (10)
                         

                        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

                        cDNA clones, fully sequenced
                        BDGP DGC clones
                        Other clones
                        Drosophila Genomics Resource Center cDNA clones

                        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                        cDNA Clones, End Sequenced (ESTs)
                        BDGP DGC clones
                          Other clones
                          RNAi and Array Information
                          Linkouts
                          DRSC - Results frm RNAi screens
                          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                          Antibody Information
                          Laboratory Generated Antibodies
                           
                          Commercially Available Antibodies
                           
                          Other Information
                          Relationship to Other Genes
                          Source for database identify of
                          Source for database merge of

                          Source for merge of: Pkc53E CG6622

                          (Bayraktaroglu, 2000.4.13)
                          Additional comments
                          Other Comments

                          dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

                          (Kiger et al., 2003)

                          The effect of Pkc53E mediated phosphorylation on each step of Top2 catalytic cycle is analysed. As with Casein kinase II (CkIIβ and CkIIβ), stimulation of enzyme activity by Pkc53E correlates with an increased rate of Top2 catalysed ATP hydrolysis. Modification did not affect any step of the enzymes catalytic cycle that preceded the ATPase step.

                          (Corbett et al., 1993)

                          Activation of endogenous Pka-C1, expression of wild type Tl receptors or treatment of cells with activators of Pkc53E and radical oxygen intermediates have only a marginal effects on the cellular distribution of dl protein in culture cells.

                          (Kubota et al., 1993)

                          Comparison of CpG distribution in the coding region of 121 genes from six species supports the mCpG mutational hotspot explanation of CpG suppression in methylated species at position II-III and III-I.

                          (Schorderet and Gartler, 1992)

                          inaC maps within 50kb of Pkc53E.

                          (Schaeffer et al., 1989)

                          Isolated by homology to bovine protein kinase C.

                          (Rosenthal et al., 1987)

                          Pkc53E has been cloned and characterised.

                          (Rosenthal et al., 1987)

                          The protein kinase C (PKC) enzyme in Drosophila is encoded by a gene family made up of three genes which are expressed primarily in adult flies. The deduced amino acid sequences of these genes are quite similar to each other and those of the mammalian PKC family (Schaeffer, Smith, Mardon, Quinn and Zuker, 1989). Ca++-dependent Molecular data indicate that Pkc53E lies approximately 20 kb from inaC, another PKC gene with 53E cytological location (Schaeffer, Smith, Mardon, Quinn and Zuker, 1989).

                          Structural gene for protein kinase C (PKC) that is expressed primarily in the brain of adult flies.

                          Origin and Etymology
                          Discoverer
                          Etymology
                          Identification
                          External Crossreferences and Linkouts ( 91 )
                          Sequence Crossreferences
                          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                          Other crossreferences
                          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                          DRscDB
                          EMBL-EBI Single Cell Expression Atlas
                          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                          Flygut - An atlas of the Drosophila adult midgut
                          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                          InterPro - A database of protein families, domains and functional sites
                          KEGG Genes - Molecular building blocks of life in the genomic space.
                          MARRVEL_MODEL
                          modMine - A data warehouse for the modENCODE project
                          SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                          Linkouts
                          DroID - A comprehensive database of gene and protein interactions.
                          DRSC - Results frm RNAi screens
                          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                          FlyMine - An integrated database for Drosophila genomics
                          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                          KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
                          MIST (protein-protein) - An integrated Molecular Interaction Database
                          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                          Synonyms and Secondary IDs (22)
                          Reported As
                          Symbol Synonym
                          CG6622
                          (Kanamori et al., 2013, Ni et al., 2010.12.1, Perkins et al., 2009, Morrison et al., 2000)
                          DPKC
                          (Llamazares et al., 1991)
                          Dpkc1
                          (Franke et al., 1994)
                          PK-C
                          (Brubaker-Purkey and Woodruff, 2013)
                          PKC
                          (Voolstra and Huber, 2020, Getahun et al., 2013, Sun et al., 2007, Roderick and Bootman, 2003, Voets and Nilius, 2003, Pandur et al., 2002, Hardie and Raghu, 2001, Hoyos et al., 2000, Broughton et al., 1995, Kubota et al., 1993, Schorderet and Gartler, 1992, Rosenthal, 1987.5.8, Rosenthal, 1987.5.8)
                          PKC 53B
                          (Morrison et al., 2000)
                          PKC 53E
                          (Wodarz et al., 2000)
                          PKC-53B
                          (Bjorklund et al., 2006)
                          PKC-53E
                          (Bjorklund et al., 2006)
                          PKC1
                          (Lee et al., 2015, Smith et al., 1991)
                          PKC53E
                          (Chvilicek et al., 2020, Li et al., 2020, Maier et al., 2019, Xi et al., 2017, Getahun et al., 2016, Otani et al., 2016, Wang et al., 2012, Murillo-Maldonado et al., 2011, Chen et al., 2010, Chen et al., 2008, Betson and Settleman, 2007, Shieh et al., 2002)
                          PKC53e
                          (Colomb and Brembs, 2016)
                          Pkc1
                          (Lindsley and Zimm, 1992)
                          Pkc53E
                          (Jafari and Alenius, 2021, Liu and Han, 2021.4.14, Vrailas-Mortimer et al., 2021, Kennison, 2020.10.22, Park et al., 2020, Donde et al., 2019, Rao and Deng, 2019.10.23, Xu et al., 2019, Zheng, 2019.3.1, Al-Ramahi et al., 2018, Lee et al., 2018, Transgenic RNAi Project members, 2017-, Gene Disruption Project members, 2016-, Gene Disruption Project members, 2015-, Ashwal-Fluss et al., 2014, Izutsu et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Mizrak et al., 2012, Korcsmáros et al., 2011, Sinenko et al., 2010, Bao and Friedrich, 2009, Wijnen et al., 2006)
                          dPKC
                          (Siegfried et al., 1990, Schaeffer et al., 1989, Rosenthal et al., 1987)
                          dPKC53E(br)
                          (Franke et al., 1994, Schaeffer et al., 1989)
                          pck53E
                          (Kanamori et al., 2013)
                          pkc53e
                          (Goel et al., 2019, Mattila et al., 2008)
                          Name Synonyms
                          Protein C kinase 53E
                          Protein kinase-C 53E
                          (Sinenko et al., 2010)
                          Protein kinase-c1
                          (Lindsley and Zimm, 1992)
                          protein kinase C
                          (Anonymous, 2003, Dworak and Sink, 2002, Dan et al., 2001, Hoyos et al., 2000, Corbett et al., 1993)
                          Secondary FlyBase IDs
                          • FBgn0034167
                          Datasets (0)
                          Study focus (0)
                          Experimental Role
                          Project
                          Project Type
                          Title
                          References (122)