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Gene Dmel\Pkc53E

General Information
Symbol	Dmel\Pkc53E	Species	D. melanogaster
Name	Protein C kinase 53E	Annotation symbol	CG6622
Feature type	protein_coding_gene	FlyBase ID	FBgn0003091
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	PKC, DPKC, dPKC53E(br), PKC1
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	53E2-53E2	Sequence location	2R:12,812,160..12,832,631 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Protein C kinase 53E is referred to in FlyBase by the symbol Dmel\Pkc53E (CG6622, FBgn0003091). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein serine/threonine kinase activity. There is experimental evidence that it is involved in the biological process: protein phosphorylation; regulation of hemocyte proliferation. 8 alleles are reported. The phenotypes of these alleles are annotated with: wing margin; wing blade. It has 6 annotated transcripts and 6 annotated polypeptides. Protein features are: AGC-kinase, C-terminal; C2 calcium-dependent membrane targeting; C2 calcium/lipid-binding domain, CaLB; C2 domain; C2 membrane targeting protein; Diacylglycerol/phorbol-ester binding; Protein kinase C, alpha/beta/gamma types; Protein kinase C-like, phorbol ester/diacylglycerol binding; Protein kinase, ATP binding site; Protein kinase, C-terminal; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine- / dual specificity protein kinase, catalytic domain; Serine/threonine-protein kinase, active site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages, during early larval stages, during late pupal stages, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult eye, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult central nervous system, adult hindgut. Comments on Affy2 ProbeSet: ProbeSet 1632478_a_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Pkc53E. Gene sequence location is 2R:12812160..12832631.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Pkc1: Protein kinase-c1 Structural gene for protein kinase C (PKC) that is expressed primarily in the brain of adult flies.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 53E2-53E2   Limits computationally determined from genome sequence between P{lacW}CG6426k10209&P{lacW}vegk07202 and P{lacW}l(2)k07408k07408
Experimentally Determined Cytological Location
Cytogenetic map Notes References 53E4-53E7     (Schaeffer et al., 1989) 53E-53E   (determined by in situ hybridisation)   (Rosenthal et al., 1987)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Pkc53E for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters. Gene model reviewed during 5.45
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Pkc53E-RA FBtr0087049  NM_057334   3175   670 Pkc53E-RB FBtr0087048  NM_166201   2696   679 Pkc53E-RC FBtr0112819  NM_001103880   2693   678 Pkc53E-RD FBtr0305069  NM_001259448   2024   525 Pkc53E-RE FBtr0305070  NM_001259449   2633   678 Pkc53E-RF FBtr0331960  NM_001274106   4711   670
Additional Transcript Data & Comments
Reported size (kB)	4.3, 4.0, 2.4 (northern blot) (Rosenthal et al., 1987)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Pkc53E-PA   FBpp0086198   76.7   670   7.15     NP_476682   AAF57932 Pkc53E-PB   FBpp0086197   77.7   679   7.14     NP_725626   AAF57933 Pkc53E-PC   FBpp0111731   77.7   678   7.67     NP_001097350   ABV53829 Pkc53E-PD   FBpp0293606   60.4   525   6.27     NP_001246377   AFH08130 Pkc53E-PE   FBpp0293607   77.7   678   7.67     NP_001246378   AFH08131 Pkc53E-PF   FBpp0304291   76.7   670   7.15     NP_001261035   AGB93567
Additional Polypeptide Data & Comments
Reported size (kDa)	670 (aa) (Schaeffer, 1998.11.23) 639 (aa); 75 (kD predicted) (Rosenthal et al., 1987)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • C2 membrane targeting protein (IPR018029) C2 calcium-dependent membrane targeting (IPR000008) Protein kinase, catalytic domain (IPR000719) AGC-kinase, C-terminal (IPR000961) Protein kinase C-like, phorbol ester/diacylglycerol binding (IPR002219) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) C2 calcium/lipid-binding domain, CaLB (IPR008973) Protein kinase-like domain (IPR011009) Protein kinase C, alpha/beta/gamma types (IPR014375) Protein kinase, ATP binding site (IPR017441) Protein kinase, C-terminal (IPR017892) Diacylglycerol/phorbol-ester binding (IPR020454) C2 domain (IPR020477)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE013599 AAF57932   AE013599 AAF57933   AE013599 ABV53829   AC004641   AE013599 AFH08130   AE013599 AFH08131   AE013599 AGB93567   AI063400   AW940124   AY095003 AAM11331   BT120171   CO190882   CO267411   CO281952   CO288662   CO298806   CO302940   CO311265   CO314960   CO316819   CO316988   CO317890   CO321656   CO345556   EC050990   EC051811   EC054980   EC055810   EC056128   EC056642   EC073802   EC080227   EC200478   EC210511   EC215422   EC223348   EC229587   EC238551   EC239531   EC248090   EC248795   EC250014   EL873323   X05076 CAA28736   X05278   X05279 CAA28890   X05280 CAA28890   X05281 CAA28890   X05282 CAA28890   X05283 CAA28890
UniProtKB/Swiss-Prot	P05130
UniProtKB/TrEMBL	A8DYG9
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference adult stage   adult brain   (Schaeffer et al., 1989)   (Rosenthal et al., 1987) retina   (Schaeffer et al., 1989) adult head   (Schaeffer et al., 1989)
Additional Descriptive Data
	Pkc53E transcripts are detected in all head neural tissue by in situ hybridization. They are observed in RNA from adult head but not adult body on northern blots. (Schaeffer et al., 1989) Pkc53E transcripts are detected in adult RNA but not in RNA from 0-3hr embryos. (Rosenthal et al., 1987)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult eye, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult central nervous system, adult hindgut. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    60.05 Larval Midgut    7.7 Larval Hindgut    46 Larval Malpighian Tubules    13.3 Larval Fat Body    5.7 Larval Salivary Gland    29.6 Larval Trachea    27.575 Larval Carcass    23.675 Adult Head    123.6 Adult Eye    862.625 Adult Brain    335.8 Adult Thoracic-Abdominal Ganglion    192.6 Adult Crop    8.8 Adult Midgut    14.8 Adult Hindgut    179.3 Adult Malpighian Tubules    7.5 Adult Fat Body    14.1 Adult Salivary Gland    16.6 Adult Heart    73.2 Adult VirginFemale Spermatheca    8.7 Adult InseminatedFemale Spermatheca    12.1 Adult Ovary    23.4 Adult Testis    6.6 Adult Male Accessory Gland    933.9 Adult Carcass    25.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    60.05 Larval Midgut    7.7 Larval Hindgut    46 Larval Malpighian Tubules    13.3 Larval Fat Body    5.7 Larval Salivary Gland    29.6 Larval Trachea    27.575 Larval Carcass    23.675 Adult Head    123.6 Adult Eye  (862.625) Adult Brain    335.8 Adult Thoracic-Abdominal Ganglion    192.6 Adult Crop    8.8 Adult Midgut    14.8 Adult Hindgut    179.3 Adult Malpighian Tubules    7.5 Adult Fat Body    14.1 Adult Salivary Gland    16.6 Adult Heart    73.2 Adult VirginFemale Spermatheca    8.7 Adult InseminatedFemale Spermatheca    12.1 Adult Ovary    23.4 Adult Testis    6.6 Adult Male Accessory Gland  (933.9) Adult Carcass    25.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    60.05 Larval Midgut    7.7 Larval Hindgut    46 Larval Malpighian Tubules    13.3 Larval Fat Body    5.7 Larval Salivary Gland    29.6 Larval Trachea    27.575 Larval Carcass    23.675 Adult Head    123.6 Adult Eye    862.625 Adult Brain    335.8 Adult Thoracic-Abdominal Ganglion    192.6 Adult Crop    8.8 Adult Midgut    14.8 Adult Hindgut    179.3 Adult Malpighian Tubules    7.5 Adult Fat Body    14.1 Adult Salivary Gland    16.6 Adult Heart    73.2 Adult VirginFemale Spermatheca    8.7 Adult InseminatedFemale Spermatheca    12.1 Adult Ovary    23.4 Adult Testis    6.6 Adult Male Accessory Gland    933.9 Adult Carcass    25.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    60.05 Larval Midgut    7.7 Larval Hindgut    46 Larval Malpighian Tubules    13.3 Larval Fat Body    5.7 Larval Salivary Gland    29.6 Larval Trachea    27.575 Larval Carcass    23.675 Adult Head    123.6 Adult Eye    862.625 Adult Brain    335.8 Adult Thoracic-Abdominal Ganglion    192.6 Adult Crop    8.8 Adult Midgut    14.8 Adult Hindgut    179.3 Adult Malpighian Tubules    7.5 Adult Fat Body    14.1 Adult Salivary Gland    16.6 Adult Heart    73.2 Adult VirginFemale Spermatheca    8.7 Adult InseminatedFemale Spermatheca    12.1 Adult Ovary    23.4 Adult Testis    6.6 Adult Male Accessory Gland    933.9 Adult Carcass    25.9 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    60.05 Larval Midgut    7.7 Larval Hindgut    46 Larval Malpighian Tubules    13.3 Larval Fat Body    5.7 Larval Salivary Gland    29.6 Larval Trachea    27.575 Larval Carcass    23.675 Adult Head    123.6 Adult Eye    862.625 Adult Brain    335.8 Adult Thoracic-Abdominal Ganglion    192.6 Adult Crop    8.8 Adult Midgut    14.8 Adult Hindgut    179.3 Adult Malpighian Tubules    7.5 Adult Fat Body    14.1 Adult Salivary Gland    16.6 Adult Heart    73.2 Adult VirginFemale Spermatheca    8.7 Adult InseminatedFemale Spermatheca    12.1 Adult Ovary    23.4 Adult Testis    6.6 Adult Male Accessory Gland    933.9 Adult Carcass    25.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    60.05 Larval Midgut    7.7 Larval Hindgut    46 Larval Malpighian Tubules    13.3 Larval Fat Body    5.7 Larval Salivary Gland    29.6 Larval Trachea    27.575 Larval Carcass    23.675 Adult Head    123.6 Adult Eye  (862.625) Adult Brain    335.8 Adult Thoracic-Abdominal Ganglion    192.6 Adult Crop    8.8 Adult Midgut    14.8 Adult Hindgut    179.3 Adult Malpighian Tubules    7.5 Adult Fat Body    14.1 Adult Salivary Gland    16.6 Adult Heart    73.2 Adult VirginFemale Spermatheca    8.7 Adult InseminatedFemale Spermatheca    12.1 Adult Ovary    23.4 Adult Testis    6.6 Adult Male Accessory Gland  (933.9) Adult Carcass    25.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    60.05 Larval Midgut    7.7 Larval Hindgut    46 Larval Malpighian Tubules    13.3 Larval Fat Body    5.7 Larval Salivary Gland    29.6 Larval Trachea    27.575 Larval Carcass    23.675 Adult Head    123.6 Adult Eye    862.625 Adult Brain    335.8 Adult Thoracic-Abdominal Ganglion    192.6 Adult Crop    8.8 Adult Midgut    14.8 Adult Hindgut    179.3 Adult Malpighian Tubules    7.5 Adult Fat Body    14.1 Adult Salivary Gland    16.6 Adult Heart    73.2 Adult VirginFemale Spermatheca    8.7 Adult InseminatedFemale Spermatheca    12.1 Adult Ovary    23.4 Adult Testis    6.6 Adult Male Accessory Gland    933.9 Adult Carcass    25.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    60.05 Larval Midgut    7.7 Larval Hindgut    46 Larval Malpighian Tubules    13.3 Larval Fat Body    5.7 Larval Salivary Gland    29.6 Larval Trachea    27.575 Larval Carcass    23.675 Adult Head    123.6 Adult Eye    862.625 Adult Brain    335.8 Adult Thoracic-Abdominal Ganglion    192.6 Adult Crop    8.8 Adult Midgut    14.8 Adult Hindgut    179.3 Adult Malpighian Tubules    7.5 Adult Fat Body    14.1 Adult Salivary Gland    16.6 Adult Heart    73.2 Adult VirginFemale Spermatheca    8.7 Adult InseminatedFemale Spermatheca    12.1 Adult Ovary    23.4 Adult Testis    6.6 Adult Male Accessory Gland    933.9 Adult Carcass    25.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    123.6   NA  Eye    862.625   NA  Brain    335.8   60.05  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    192.6   NA  Crop    8.8   7.7  Midgut    no informative data   46  Hindgut    179.3   no informative data  Malpighian Tubules    7.5   5.7  Fat Body    no informative data   29.6  Salivary Gland    no informative data   NA  Heart    73.2   27.575  Trachea    NA   NA  VirginFemale Spermatheca    8.7   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    23.4   NA  Testis    6.6   NA  Male Accessory Gland    933.9   23.675  Carcass    25.9 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    5 central nervous system, pupae P8    37 head, virgin 1-day female    31 head, virgin 4-day female    29 head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female    26 head, mated 20-day female    18 head, mated 1-day male    40 head, mated 4-day male    47 head, mated 20-day male    43 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    1 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    0 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    5 central nervous system, pupae P8  (37) head, virgin 1-day female  (31) head, virgin 4-day female  (29) head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female  26 head, mated 20-day female    18 head, mated 1-day male  (40) head, mated 4-day male  (47) head, mated 20-day male  (43) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    1 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    0 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    5 central nervous system, pupae P8    37 head, virgin 1-day female    31 head, virgin 4-day female    29 head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female    26 head, mated 20-day female    18 head, mated 1-day male    40 head, mated 4-day male    47 head, mated 20-day male    43 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    1 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    0 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    5 central nervous system, pupae P8    37 head, virgin 1-day female    31 head, virgin 4-day female    29 head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female    26 head, mated 20-day female    18 head, mated 1-day male    40 head, mated 4-day male    47 head, mated 20-day male    43 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    1 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    0 accessory gland, mated 4-day male    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    5 central nervous system, pupae P8    37 head, virgin 1-day female    31 head, virgin 4-day female    29 head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female    26 head, mated 20-day female    18 head, mated 1-day male    40 head, mated 4-day male    47 head, mated 20-day male    43 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    1 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    0 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    5 central nervous system, pupae P8  (37) head, virgin 1-day female  31 head, virgin 4-day female  29 head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female  26 head, mated 20-day female    18 head, mated 1-day male  (40) head, mated 4-day male  (47) head, mated 20-day male  (43) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    1 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    0 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    5 central nervous system, pupae P8    37 head, virgin 1-day female    31 head, virgin 4-day female    29 head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female    26 head, mated 20-day female    18 head, mated 1-day male    40 head, mated 4-day male    47 head, mated 20-day male    43 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    1 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    0 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    5 central nervous system, pupae P8    37 head, virgin 1-day female    31 head, virgin 4-day female    29 head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female    26 head, mated 20-day female    18 head, mated 1-day male    40 head, mated 4-day male    47 head, mated 20-day male    43 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    1 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    0 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages, during early larval stages, during late pupal stages, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    1 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    5 embryo 22-24hr    8 larva L1    6 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    5 pupae 3d postWPP    8 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    5 adult male 30day    4 adult female 01day    2 adult female 05day    2 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    1 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    5 embryo 22-24hr    8 larva L1    6 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    5 pupae 3d postWPP    8 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    5 adult male 30day    4 adult female 01day    2 adult female 05day    2 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    1 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    5 embryo 22-24hr    8 larva L1    6 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    5 pupae 3d postWPP    8 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    5 adult male 30day    4 adult female 01day    2 adult female 05day    2 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    1 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    5 embryo 22-24hr    8 larva L1    6 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    5 pupae 3d postWPP    8 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    5 adult male 30day    4 adult female 01day    2 adult female 05day    2 adult female 30day    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    1 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    5 embryo 22-24hr    8 larva L1    6 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    5 pupae 3d postWPP    8 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    5 adult male 30day    4 adult female 01day    2 adult female 05day    2 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    1 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    5 embryo 22-24hr    8 larva L1    6 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    5 pupae 3d postWPP    8 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    5 adult male 30day    4 adult female 01day    2 adult female 05day    2 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    1 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    5 embryo 22-24hr    8 larva L1    6 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    5 pupae 3d postWPP    8 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    5 adult male 30day    4 adult female 01day    2 adult female 05day    2 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    1 embryo 16-18hr    3 embryo 18-20hr    4 embryo 20-22hr    5 embryo 22-24hr    8 larva L1    6 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    5 pupae 3d postWPP    8 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    5 adult male 30day    4 adult female 01day    2 adult female 05day    2 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    7 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    7 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    7 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    7 ovary OSS    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    7 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    7 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    7 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    7 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_18 mE1_20_mRNA_expression_cluster_11
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003091 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6622-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Pkc53EEY14093 viable, with Scer\GAL4pnr-MD237 Pkc53EGD11984 Sterility Allele fertile Pkc53EEY14093 Other Phenotypes Allele size defective | adult stage | dominant Pkc53EEY14093 visible, with Scer\GAL4Act88F.PD Pkc53Ekin.Scer\UAS visible, with Scer\GAL4Cy6 Pkc53Ekin.Scer\UAS Phenotype manifest in Allele wing blade Pkc53EEY14093 wing margin, with Scer\GAL4Cy6 Pkc53Ekin.Scer\UAS wing vein | ectopic Pkc53EEY14093 wing vein | ectopic, with Scer\GAL4Act88F.PD Pkc53Ekin.Scer\UAS
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Pkc53E Class Mutagen Stocks Known lesion Pkc53EEY14093 P-element activity 1 -- Pkc53EMB02781 1 -- Pkc53EMI05296 1 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Pkc53E Class Mutagen Stocks Known lesion Pkc53EGD11984 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Pkc53EHMS01195 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Pkc53EJF02641 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Pkc53Ekin.Scer\UAS in vitro construct - regulatory fusion 0 Yes Pkc53ENIG.6622R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel6065 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11984} NA P{NIG.6622R} NA P{TRiP.HMS01195} NA P{TRiP.JF02641} NA P{UAS-Pkc53E.kin} NA characterization construct P{hs-Pak-53E} NA P{hs-Pkn-PKC53E} NA P{hs-Rhot-53E} NA P{hs-Rok-53E} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}Pkc53EEY14093 NA insertion of enhancer trap binary system Mi{ET1}Pkc53EMB02781 No miscellaneous insertions Mi{MIC}Pkc53EMI05296 NA
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function
CV term Evidence References
protein serine/threonine kinase activity inferred from direct assay (Corbett et al., 1993)
Biological Process
CV term Evidence References
protein phosphorylation inferred from direct assay (Corbett et al., 1993) regulation of hemocyte proliferation inferred from mutant phenotype (Sinenko et al., 2010)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR000961, InterPro:IPR002290, InterPro:IPR014375, InterPro:IPR017441, InterPro:IPR017892 (FlyBase Curators et al., 2004-) calcium-dependent protein kinase C activity non-traceable author statement (Swiss-Prot Project Members, 1987.8.13) diacylglycerol binding inferred from sequence or structural similarity with InterPro:IPR002219 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1987.8.13) protein kinase C activity inferred from electronic annotation with InterPro:IPR014375 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Rosenthal et al., 1987) non-traceable author statement (Swiss-Prot Project Members, 1987.8.13) protein serine/threonine kinase activity non-traceable author statement (Morrison et al., 2000) zinc ion binding inferred from electronic annotation with InterPro:IPR014375 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
intracellular signal transduction inferred from electronic annotation with InterPro:IPR002219 (FlyBase Curators et al., 2004-) protein phosphorylation non-traceable author statement (Morrison et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Pkc53E - CG1444 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pkc53E - CG8545 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pkc53E - Elongin-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pkc53E - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pkc53E - Hsp23 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pkc53E - Hsp70Aa anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pkc53E - Ns1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pkc53E - Tango4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pkc53E - Trap1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pkc53E - inaC anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pkc53E - nop5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Pkc53E - vig anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Pkc53E allele Gene References
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0003091 DroID - A comprehensive database of gene and protein interactions. • FBgn0003091 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 48311 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P05130
Orthologs
OrthoDB Orthologs (84) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6N050D Dipteran inclusive ortholog search EOG63JCCS Insect inclusive ortholog search EOG65HQSC Arthropod inclusive ortholog search EOG6V6WXS Metazoa inclusive ortholog search EOG61KB55
Orthologs in Drosophila Species (EOG6N050D)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Pkc53E     Drosophila simulans   Dsim\GD11233 Y   Drosophila sechellia   Dsec\GM21740     Drosophila erecta   Dere\GG20645     Drosophila yakuba   Dyak\GE11630 Y   Drosophila ananassae   Dana\GF11424 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA19732     Drosophila persimilis   Dper\GL11571 Y   Drosophila willistoni   Dwil\GK22032 Y   Drosophila virilis   Dvir\GJ21016 Y   Drosophila mojavensis   Dmoj\GI21163 Y   Drosophila grimshawi   Dgri\GH22650 Y
Orthologs in non-Drosophila Dipterans (EOG63JCCS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL001108   Anopheles gambiae Malaria mosquito  Agam\AGAP012252   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ005701
Orthologs in non-Dipteran Insects (EOG65HQSC)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB43222   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG009095   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10293   Atta cephalotes Leafcutter ant  Acep\ACEP20878   Camponotus floridanus Florida carpenter ant  Cflo\CFLO16197   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL13050   Linepithema humile Argentine ant  Lhum\LH11001   Pogonomyrmex barbatus Red harvester ant  Pbar\PB17933   Solenopsis invicta Red fire ant  Sinv\SINV10292   Acyrthosiphon pisum Pea aphid  Apim\ACYPI002684   Bombyx mori Silkmoth  Bmor\BGIBMGA014132   Pediculus humanus Human body louse  Phum\PHUM528520   Tribolium castaneum Red flour beetle  Tcas\TC003019
Orthologs in non-Insect Arthropods (EOG6V6WXS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_326383 Y Daphnia pulex Water flea  Dpux\Tssk1b Y Ixodes scapularis Deer tick  Isca\ISCW020000 Y Ixodes scapularis Deer tick  Isca\ISCW004410 Y
Orthologs in non-Arthropod Metazoa (EOG61KB55)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\E01H11.1 Y Caenorhabditis elegans Nematode  Cele\pkc-1 Y Caenorhabditis elegans Nematode  Cele\tpa-1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_027848gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001049gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_008678gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_026498gn Y Danio rerio Zebrafish  Drer\prkcba Y Danio rerio Zebrafish  Drer\prkcq Y Danio rerio Zebrafish  Drer\BX005175.1 Y Danio rerio Zebrafish  Drer\prkceb Y Danio rerio Zebrafish  Drer\prkcea Y Danio rerio Zebrafish  Drer\prkchb Y Danio rerio Zebrafish  Drer\prkcbb Y Danio rerio Zebrafish  Drer\prkcg Y Danio rerio Zebrafish  Drer\prkca Y Danio rerio Zebrafish  Drer\ENSDARG00000086250 Y Danio rerio Zebrafish  Drer\prkcha Y Danio rerio Zebrafish  Drer\prkcd Y Xenopus tropicalis Western clawed frog  Xtro\prkcb Y Xenopus tropicalis Western clawed frog  Xtro\prkcd Y Xenopus tropicalis Western clawed frog  Xtro\prkch Y Xenopus tropicalis Western clawed frog  Xtro\prkcg Y Xenopus tropicalis Western clawed frog  Xtro\prkcq Y Xenopus tropicalis Western clawed frog  Xtro\prkce Y Gallus gallus Domestic chicken  Ggal\PRKCD Y Gallus gallus Domestic chicken  Ggal\PRKCA Y Gallus gallus Domestic chicken  Ggal\PRKCB Y Gallus gallus Domestic chicken  Ggal\PRKCH Y Gallus gallus Domestic chicken  Ggal\ENSGALG00000023876 Y Gallus gallus Domestic chicken  Ggal\PRKCE Y Mus musculus House mouse  Mmus\Prkcq Y Mus musculus House mouse  Mmus\Prkch Y Mus musculus House mouse  Mmus\ENSMUSG00000021948 Y Mus musculus House mouse  Mmus\Prkce Y Mus musculus House mouse  Mmus\Prkcc Y Mus musculus House mouse  Mmus\ENSMUSG00000052889 Y Mus musculus House mouse  Mmus\Prkca Y Rattus norvegicus Norway rat  Rnor\Prkch Y Rattus norvegicus Norway rat  Rnor\Prkca Y Rattus norvegicus Norway rat  Rnor\Prkce Y Rattus norvegicus Norway rat  Rnor\Prkcg Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000012061 Y Rattus norvegicus Norway rat  Rnor\Prkcd Y Rattus norvegicus Norway rat  Rnor\Prkcq Y Homo sapiens Human  Hsap\PRKCQ Y Homo sapiens Human  Hsap\PRKCD Y Homo sapiens Human  Hsap\PRKCE Y Homo sapiens Human  Hsap\PRKCH Y Homo sapiens Human  Hsap\PRKCA Y Homo sapiens Human  Hsap\PRKCG Y Homo sapiens Human  Hsap\PRKCB Y
Human Orthologs (7)
Gene OMIM HGNC Hsap\PRKCQ     Hsap\PRKCD     Hsap\PRKCE     Hsap\PRKCH     Hsap\PRKCA     Hsap\PRKCG     Hsap\PRKCB
AAA Orthologs (8) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD11233 Drosophila yakuba Dyak\GE11630 Drosophila ananassae Dana\GF11424 Drosophila persimilis Dper\GL11571 Drosophila willistoni Dwil\GK22032 Drosophila virilis Dvir\GJ21016 Drosophila mojavensis Dmoj\GI21163 Drosophila grimshawi Dgri\GH22650
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	24443 w1118; Mi{ET1}Pkc53EMB02781 27491 y1 v1; P{TRiP.JF02641}attP2 42309 y1 w*; Mi{MIC}Pkc53EMI05296 34716 y1 sc* v1; P{TRiP.HMS01195}attP2 20790 y1 w67c23; P{EPgy2}Pkc53EEY14093/CyO
VDRC	v27696 w1118; P{GD11984}v27696/TM3
Genomic Clones ( 1 )
	BACR09M08 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 23 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH03188
Other clones	DPiM_GH03188 MIP15613
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	EC050990 EC051811 EC054980 EC055810 EC056128 EC056642 EK050002 EK069106 EK150049 EK159158 EK173912 EK185217 EK186120 EK223961 EK238615 EK265953 EK266530 EK269634 EP22257 IP05164
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003091 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 48311
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Pkc53E CG6622 (Bayraktaroglu, 2000.4.13)
Additional comments
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) The effect of Pkc53E mediated phosphorylation on each step of Top2 catalytic cycle is analysed. As with Casein kinase II (CkIIβ and CkIIβ), stimulation of enzyme activity by Pkc53E correlates with an increased rate of Top2 catalysed ATP hydrolysis. Modification did not affect any step of the enzymes catalytic cycle that preceded the ATPase step. (Corbett et al., 1993) Activation of endogenous Pka-C1, expression of wild type Tl receptors or treatment of cells with activators of Pkc53E and radical oxygen intermediates have only a marginal effects on the cellular distribution of dl protein in culture cells. (Kubota et al., 1993) Comparison of CpG distribution in the coding region of 121 genes from six species supports the mCpG mutational hotspot explanation of CpG suppression in methylated species at position II-III and III-I. (Schorderet and Gartler, 1992) inaC maps within 50kb of Pkc53E. (Schaeffer et al., 1989) Isolated by homology to bovine protein kinase C. (Rosenthal et al., 1987) Pkc53E has been cloned and characterised. (Rosenthal et al., 1987) The protein kinase C (PKC) enzyme in Drosophila is encoded by a gene family made up of three genes which are expressed primarily in adult flies. The deduced amino acid sequences of these genes are quite similar to each other and those of the mammalian PKC family (Schaeffer, Smith, Mardon, Quinn and Zuker, 1989). Ca++-dependent Molecular data indicate that Pkc53E lies approximately 20 kb from inaC, another PKC gene with 53E cytological location (Schaeffer, Smith, Mardon, Quinn and Zuker, 1989).   Structural gene for protein kinase C (PKC) that is expressed primarily in the brain of adult flies.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057334 NM_166201 NM_001103880 NM_001259448 NM_001259449 NM_001274106 RefSeq (Proteins) • NP_476682 NP_725626 NP_001097350 NP_001246377 NP_001246378 NP_001261035 DDBJ / EMBL / GenBank • AAF57932 AAF57933 AAM11331 ABV53829 AC004641 AFH08130 AFH08131 AGB93567 AI063400 AW940124 AY095003 CAA28736 CAA28890 X05076 X05278 X05279 X05280 X05281 X05282 X05283 Entrez Gene - A searchable database of RefSeq genes. • 48311 UniProtKB/Swiss-Prot • P05130 UniProtKB/TrEMBL • A8DYG9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • C2 membrane targeting protein (IPR018029) C2 calcium-dependent membrane targeting (IPR000008) Protein kinase, catalytic domain (IPR000719) AGC-kinase, C-terminal (IPR000961) Protein kinase C-like, phorbol ester/diacylglycerol binding (IPR002219) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) C2 calcium/lipid-binding domain, CaLB (IPR008973) Protein kinase-like domain (IPR011009) Protein kinase C, alpha/beta/gamma types (IPR014375) Protein kinase, ATP binding site (IPR017441) Protein kinase, C-terminal (IPR017892) Diacylglycerol/phorbol-ester binding (IPR020454) C2 domain (IPR020477)
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0003091 DroID - A comprehensive database of gene and protein interactions. • FBgn0003091 DRSC - Results from RNAi screens. • FBgn0003091 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003091 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6622-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003091 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 48311 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/protknc1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 48311 modMine - Data generated by the modENCODE project. • FBgn0003091 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P05130
Synonyms & Secondary IDs ( 19 )
Reported As
Symbol Synonym	CG6622 (Morrison et al., 2000, Perkins et al., 2009, Ni et al., 2010.12.1) DPKC (Llamazares et al., 1991) dPKC (Siegfried et al., 1990, Schaeffer et al., 1989, Rosenthal et al., 1987) Dpkc1 (Franke et al., 1994) dPKC53E(br) (Franke et al., 1994, Schaeffer et al., 1989, ) PKC (Roderick and Bootman, 2003, Voets and Nilius, 2003, Pandur et al., 2002, Hardie and Raghu, 2001, Rosenthal, 1987.5.8, Rosenthal, 1987.5.8, Broughton et al., 1995, Kubota et al., 1993, Schorderet and Gartler, 1992, Hoyos et al., 2000, Sun et al., 2007) PKC1 (Smith et al., 1991) Pkc1 (Lindsley and Zimm, 1992) PKC-53B (Bjorklund et al., 2006) PKC 53B (Morrison et al., 2000) PKC-53E (Bjorklund et al., 2006) PKC 53E (Wodarz et al., 2000) Pkc53E (Wijnen et al., 2006, Izutsu et al., 2012, Sinenko et al., 2010, Bao and Friedrich, 2009, Korcsmáros et al., 2011, Mizrak et al., 2012, Japanese National Institute of Genetics, 2012.5.21) PKC53E (Betson and Settleman, 2007, Chen et al., 2010, Chen et al., 2008, Murillo-Maldonado et al., 2011) pkc53e (Mattila et al., 2008)
Name Synonym	Protein C kinase 53E   protein kinase C (Anonymous, 2003, Dworak and Sink, 2002, Dan et al., 2001, Corbett et al., 1993, Hoyos et al., 2000) Protein kinase-c1 (Lindsley and Zimm, 1992) Protein kinase-C 53E (Sinenko et al., 2010)
Secondary FlyBase IDs
	FBgn0034167
References ( 68 )
Generate a list of	All references relating to this gene ( 68 )
List References by type	Research paper  ( 26 ) Supplementary material  ( 5 ) Review  ( 10 ) Personal communication to FlyBase  ( 14 ) Abstract  ( 4 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Book  ( 1 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 4 )
	Izutsu et al., 2012, PLoS ONE 7(3): e33288 Genome features of "dark-fly", a Drosophila line reared long-term in a dark environment. [FBrf0217825] Mizrak et al., 2012, Curr. Biol. 22(20): 1871--1880 Electrical activity can impose time of day on the circadian transcriptome of pacemaker neurons. [FBrf0219786] Korcsmáros et al., 2011, PLoS ONE 6(5): e19240 Signalogs: orthology-based identification of novel signaling pathway components in three metazoans. [FBrf0213696] Murillo-Maldonado et al., 2011, PLoS ONE 6(11): e28067 Insulin Receptor-Mediated Signaling via Phospholipase C-γ Regulates Growth and Differentiation in Drosophila. [FBrf0216849] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...