A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\pn

General Information
SymbolDmel\pnSpeciesD. melanogaster
NamepruneAnnotation symbolCG3461
Feature typeprotein_coding_geneFlyBase IDFBgn0003116
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)XRecombination map1-0.8
Cytogenetic map2E1-2E1Sequence locationX:2,075,127..2,076,972 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene prune is referred to in FlyBase by the symbol pn (CG3461, FBgn0003116). It has the cytological map location 2E1. Its sequence location is X:2075127..2076972. Its molecular function is described as: pyrophosphatase activity; manganese ion binding. It is involved in the biological process compound eye pigmentation. 105 alleles are reported. The phenotype of these alleles is annotated with pigment cell. It has one annotated transcript and one annotated polypeptide.

hide Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage
pn: prune
Eye color of newly emerged flies transparent brownish red, becoming brownish purple with age. The eyes of pn males have about 25% as much drosopterin (red pigment) as the eyes of wild-type males (Gearhart and MacIntyre, 1970, Anal. Biochem. 37: 21-25); the concentrations of xanthopterin and sepiapterin (brown pigments) are increased to about 110% of wild-type (Nolte, 1959). Control of drosopterin synthesis seems to be related to the activity of the enzyme GTP cyclohydrolase (Evans and Howell, 1979, Biochem. Genet. 16: 13-26). The pn eye color is autonomous in larval optic disks transplanted into wild-type hosts (Beadle and Ephrussi, 1936). Larval Malpighian tube color is normal (Brehme and Demerec, 1942, Growth 6: 351-56). Standard pn mutants are homo- and hemizygous viable in a wild-type background, but show a lethal interaction with the third chromosome dominant awkK (Lifschytz and Falk, 1969; Orevi, 1973, DIS 50: 77). Some temperature-sensitive pn mutants (pnts-e), however, are insensitive to the killing action of awkK; one temperature-sensitive mutant (pnts-ek) is insensitive to awkK at permissive temperatures (18, 22), but sensitive to awkK at restrictive temperatures (25, 29) (Orevi, 1973, DIS 50: 80; Orevi and Falk, 1975). The TSP for the eye color phenotype occurs during the late pupal stage, while the TSP for the pn component of the pn-awkK interaction begins at the late pupal stage and lasts until eclosion (Orevi and Falk, 1975). Homo- and hemizygous pn deficiencies and other chromosomal rearrangements have been induced by Ilyina et al. (1980), as indicated in the allele table.
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
2E1-2E1  
Limits computationally determined from genome sequence between P{EP}ActnEP1193&P{EP}CG4322EP1631 and P{EP}EP1460&P{EP}CG2924EP1596  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
2E1-2E2
(determined by in situ hybridisation)  
Experimentally Determined Recombination Data
Location
1-0.8
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
In direction of increasing cytology: Cyp4ae1+ pn+ Nmd3-
In direction of increasing cytology: Pgd+ wapl- Cyp4d1- CG3621+ Cyp4ae1+ pn+ Nmd3-
In direction of increasing cytology: Pgd? l(1)2Dg? wapl? pn? l(1)2Ea? Vinc? pcx? kz? fs(1)K10? In direction of increasing cytology: wapl? Cyp4ae1? pn? In direction of increasing cytology: wapl? Cyp4d1? pn? In direction of increasing cytology: wapl? Cyp4d2? pn? In direction of increasing cytology: wapl? Cyp4d8? pn?
In direction of increasing cytology: Pgd+ bcn92- wapl- Cyp4d1- CG3630+ CG3621+ Cyp4d14- Cyp4d2+ Cyp4ae1+ pn+
Gene Order (overall orientation not stated)
References
Overall orientation not stated: Cyp4ae1+ pn+ Nmd3-
hide Gene Model & Products
Please see the GBrowse view of Dmel\pn for information on other features
detailed view FBtr0070388 FBtr0070389 FBtr0070425 FBpp0070372 FBpp0070373 FBpp0070409 FBti0014581 FBti0014580 FBti0014582 FBti0015042 FBti0015027 FBti0015026 FBti0030327 FBti0027287
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
FBtr0070389
  1778
  405
Additional Transcript Data & Comments
Reported size (kB)
1.8 (northern blot)
1.4 (longest cDNA)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
pn-PA  
FBpp0070373  
45.2  
405  
6.32  
Additional Polypeptide Data & Comments
Reported size (kD)
405 (aa); 50 (kD)
404 (aa); 44 (kD predicted)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
DHHA2 (IPR004097)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    deletion
    comment=7 bp deletion
    evidence=experimental
    insertion site
    comment=4bp insertion
    evidence=experimental
    point mutation
    comment=second of two mutations
    evidence=experimental
    pr_change=S164F|pn-PA
    na_change=C2075891T
    point mutation
    evidence=experimental
    pr_change=Q186@|pn-PA
    na_change=C2075956T
    point mutation
    evidence=experimental
    comment=first of two mutations
    pr_change=C154Y|pn-PA
    na_change=G2075861A
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data
    Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
       embryonic stage-adult stage
      Marker for
        Subcellular Localization
        CV Term
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0070388 FBtr0070389 FBtr0070425
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        Other Phenotypes
        Allele
        Phenotype manifest in
        Allele
        hide Classical Alleles ( 102 )
        For All Classical Alleles Show

        Allele of pnClassMutagenStocksKnown lesion
        pn120 --
        pn12C0 Yes
        pn18a0 Yes
        pn165 Yes
        pn1a0 --
        pn200 --
        pn250 --
        pn26-200 --
        pn260 --
        pn27-220 --
        pn27-90 --
        pn21 Yes
        pn2a0 --
        pn360 --
        pn380 Yes
        pn38r120 Yes
        pn38r130 --
        pn38r140 --
        pn38r10 Yes
        pn38r30 --
        pn38r40 Yes
        pn38r60 Yes
        pn31 --
        pn3a0 --
        pn400 --
        pn41f290 --
        pn450 --
        pn51b0 --
        pn51h0 --
        pn54c0 Yes
        pn550 --
        pn57e0 --
        pn59j0 --
        pn50 --
        pn621 --
        pn63d0 --
        pn68b
          0 --
          pn690 --
          pn77C330 Yes
          pnA0 Yes
          pnAA10 --
          pnFG0 --
          pnFS10 --
          pnGB390 Yes
          pnMS20 --
          pnU10 Yes
          pnU20 Yes
          pnU30 Yes
          pnX
            1 --
            pnlb0 --
            pnpw20 Yes
            pntr100 --
            pntr110 --
            pntr120 --
            pntr130 --
            pntr140 --
            pntr150 --
            pntr160 --
            pntr170 --
            pntr180 --
            pntr190 --
            pntr10 --
            pntr200 --
            pntr210 --
            pntr220 --
            pntr230 --
            pntr240 --
            pntr250 --
            pntr260 --
            pntr270 --
            pntr280 --
            pntr290 --
            pntr20 --
            pntr300 --
            pntr310 --
            pntr320 --
            pntr330 --
            pntr340 --
            pntr350 --
            pntr360 --
            pntr370 --
            pntr380 --
            pntr390 --
            pntr30 --
            pntr400 --
            pntr40 --
            pntr50 --
            pntr60 --
            pntr70 --
            pntr80 --
            pntr90 --
            pnts-e10 --
            pnts-e20 --
            pnts-e30 --
            pnts-e40 --
            pnts-e50 --
            pnts-e60 --
            pnts-e70 --
            pnts-e80 --
            pnts-e90 --
            pnts-ek0 --
            pnunspecified
              0 --
              hide Alleles Carried on Transgenic Constructs ( 3 )
              For All Alleles Carried on Transgenic Constructs Show

              Allele of pnClassMutagenStocksKnown lesion
              pnGD12691 Yes
              pnhs.30 Yes
              pnhs.40 Yes
              hide Aneuploid Aberrations
              Useful deficiency
              Useful duplication
              Disrupted in