transcription factor - paired domain - specifies the differences between mono-innervated external sensory (m-es) organs and poly-innervated external sensory (p-es) organs - determines the fate to form larval p-es organs and adult chemosensory bristles - and define the presumptive deutocerebral-tritocerebral boundary
Gene model reviewed during 5.44
Gene model reviewed during 5.50
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Poxn using the Feature Mapper tool.
Comment: reported as dorsal/lateral sensory complexes
Expression of Poxn begins at embryonic stages 10-11, in two stripes in the procephalic neurogenic region, which become restricted to the posterior protocerebrum and posterior deuterocerebrum. Poxn is expressed near sv, but never in the same cell.
Poxn protein is found predominantly in nuclei but occasional cytoplasmic staining is seen.
Poxn protein isexpressed in four clusters of cells per hemisegment, two each in the CNSand PNS. It is first expressed at ~5.5 hours of development in a few cellsin the gnathal segments and in a single cell of each thoracic andabdominal hemisegment. At ~5.75hr, when the single cell starts dividing, asecond, more ventral cell appears. At the same time, the first labelledneuroblasts appear in the CNS at the anterior boundary of eachhemisegment. These consist of a neuroblast or a neuroblast and its firstGMC in each hemisegment. During germ band retraction, the dorsal pair ofPNS cells migrate ventrally to assume lateral positions. At the fast stageof germ band shortening, the pattern comprises two clusters of <8 cells inthe PNS and two neuroblasts and descended cells. After stage 13, Poxnprotein is no longer detected. In the wing discs, expression is seen inthe precursors of the poly-innervated wing margin bristles which are alongthe anterior wing margin. With the exception of the Keilin\'s organs,expression is specific for poly-innervated sense organs.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Poxn in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Poxn is responsible for differentiating chemosensory organs from mechanosensory organs.
Ectopic expression of Poxn can induce the transformation of mechanosensory into chemosensory neurons. The neurons innervating the transformed organs follow a pathway typical of chemosensory neurons. Behavioural tests show that these neurons establish connections appropriate to taste-mediating bristles.
Poxn gene product has been characterised and results demonstrate that the gene product is responsible for the poly innervated external sense organs.
In larvae, deletion of Poxn results in the morphological transformation of chemosensory into mechanosensory organs; an overexpression of the same gene induces the reciprocal transformation. Overexpression of Poxn induces the morphological transformation of mechanosensory into chemosensory bristles on the adult legs; the neurons innervating the morphologically transformed bristles follow pathways and establish connections that are appropriate for chemosensory bristles.