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General Information
Symbol
Dmel\Poxn
Species
D. melanogaster
Name
Pox neuro
Annotation Symbol
CG8246
Feature Type
FlyBase ID
FBgn0003130
Gene Model Status
Stock Availability
Gene Snapshot
Pox neuro (Poxn) encodes a transcriptional factor that specifies the differences between mono-innervated external sensory (m-es) organs and poly-innervated external sensory (p-es) organs. Poxn expression in the external sensory organ precursor determines the fate to form larval p-es organs and adult chemosensory bristles. [Date last reviewed: 2019-03-14]
Also Known As

Pox-n

Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:15,826,693..15,835,055 [-]
Recombination map

2-75

RefSeq locus
NT_033778 REGION:15826693..15835055
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (10 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR001523, InterPro:IPR009057
(assigned by InterPro )
Biological Process (8 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR001523
(assigned by InterPro )
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Protein Family (UniProt)
-
Summaries
Gene Group (FlyBase)
PAIRED DOMAIN TRANSCRIPTION FACTORS -
The paired domain transcription factors are sequence-specific DNA binding proteins that regulate transcription. These members have the characteristic paired domain but lack the homeodomain. (Adapted from FBrf0232555 and FBrf0135231).
Protein Function (UniProtKB)
Transcriptional regulator that specifies poly-innervated organs (chemosensory bristle). Also controls the number of neurons.
(UniProt, P23758)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Pox-n: Paired box-neuro
Pox-n shows tissue-specific, segmentally-repeated expression in the central and peripheral nervous system at about 5 hr after fertilization. Transcripts are apparently expressed in neural precursors of the peripheral and the central nervous system. Cells expressing this gene seem to be clonally related.
Summary (Interactive Fly)

transcription factor - paired domain - specifies the differences between mono-innervated external sensory (m-es) organs and poly-innervated external sensory (p-es) organs - determines the fate to form larval p-es organs and adult chemosensory bristles - and define the presumptive deutocerebral-tritocerebral boundary

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Poxn for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0087311
2320
425
FBtr0329908
2458
425
Additional Transcript Data and Comments
Reported size (kB)

2.5 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0086446
44.3
425
6.44
FBpp0302942
44.3
425
6.44
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

425 aa isoforms: Poxn-PA, Poxn-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Poxn using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

Additional Descriptive Data

Poxn is expressed in the posterior spiracle primordium from embryonic stage 11 on.

Poxn transcripts are expressed in segmentally repeated pattern in neural precursors in both the CNS and PNS.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Expression of Poxn begins at embryonic stages 10-11, in two stripes in the procephalic neurogenic region, which become restricted to the posterior protocerebrum and posterior deuterocerebrum. Poxn is expressed near sv, but never in the same cell.

Poxn protein is found predominantly in nuclei but occasional cytoplasmic staining is seen.

Poxn protein isexpressed in four clusters of cells per hemisegment, two each in the CNSand PNS. It is first expressed at ~5.5 hours of development in a few cellsin the gnathal segments and in a single cell of each thoracic andabdominal hemisegment. At ~5.75hr, when the single cell starts dividing, asecond, more ventral cell appears. At the same time, the first labelledneuroblasts appear in the CNS at the anterior boundary of eachhemisegment. These consist of a neuroblast or a neuroblast and its firstGMC in each hemisegment. During germ band retraction, the dorsal pair ofPNS cells migrate ventrally to assume lateral positions. At the fast stageof germ band shortening, the pattern comprises two clusters of <8 cells inthe PNS and two neuroblasts and descended cells. After stage 13, Poxnprotein is no longer detected. In the wing discs, expression is seen inthe precursors of the poly-innervated wing margin bristles which are alongthe anterior wing margin. With the exception of the Keilin\'s organs,expression is specific for poly-innervated sense organs.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
Expression Deduced from Reporters
Reporter: P{Poxn-GAL4.13}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{Poxn-GAL4.14}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{Poxn-GAL4.757}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{Poxn-GAL4.brain}
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Poxn in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 10 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 33 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Poxn
Transgenic constructs containing regulatory region of Poxn
Deletions and Duplications ( 6 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
larval abdominal segment & sensillum campaniformium | ectopic
larval abdominal segment & sensillum trichodeum | ectopic
larval thoracic segment & sensillum campaniformium | ectopic
mechanosensory sensory organ & tibia & adult
mechanosensory sensory organ & tibia & neuron & adult
mesothoracic leg sensillum & tibia
mesothoracic tarsal segment & joint
wing hinge & wing vein
wing hinge & wing vein L5
wing vein L3 & macrochaeta
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (12)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
2 of 15
Yes
No
2 of 15
Yes
No
2 of 15
Yes
No
2 of 15
Yes
No
2 of 15
Yes
No
1  
1 of 15
No
Yes
2  
1 of 15
No
Yes
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
2  
1 of 15
No
No
0  
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (17)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
2 of 15
Yes
No
2 of 15
Yes
No
2 of 15
Yes
No
2 of 15
Yes
No
0  
2 of 15
Yes
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
3  
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (10)
2 of 13
Yes
No
2 of 13
Yes
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (12)
2 of 12
Yes
No
2 of 12
Yes
No
2 of 12
Yes
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (17)
3 of 15
Yes
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (11)
5 of 15
Yes
No
4 of 15
No
No
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (1)
1 of 12
Yes
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190CN2 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150BHW )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0OC1 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0OM2 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0S4E )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (23)
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map

    2-75

    Cytogenetic map
    Sequence location
    2R:15,826,693..15,835,055 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    52C7-52C7
    Limits computationally determined from genome sequence between P{lacW}Vha36k07207 and P{lacW}l(2)k05713k05713
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    52D1-52D1
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (8)
    Genomic Clones (13)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (2)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
      Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for database merge of
        Additional comments
        Other Comments

        Poxn is required for normal male courtship and fertility. Poxn function is required for the normal development of chemosensory bristles in the labellum, legs and wings.

        Poxn is responsible for differentiating chemosensory organs from mechanosensory organs.

        Poxn expression is independent of ct but dependent on the achaete-scute complex.

        The ectopic expression of Poxn transforms ch organs into es organs; this transformation can occur in achaete-scute complex- embryos but not in ct- embryos.

        The Poxn protein binds to 40 sites on the polytene chromosomes. One of the sites corresponds to the ct locus. Poxn probably has a direct effect on the ct regulatory region.

        Poxn can control the number of neurons. Poxn can act at a late stage to affect the fate of the undifferentiated neuron.

        Ectopic expression of Poxn can induce the transformation of mechanosensory into chemosensory neurons. The neurons innervating the transformed organs follow a pathway typical of chemosensory neurons. Behavioural tests show that these neurons establish connections appropriate to taste-mediating bristles.

        Poxn gene product has been characterised and results demonstrate that the gene product is responsible for the poly innervated external sense organs.

        In larvae, deletion of Poxn results in the morphological transformation of chemosensory into mechanosensory organs; an overexpression of the same gene induces the reciprocal transformation. Overexpression of Poxn induces the morphological transformation of mechanosensory into chemosensory bristles on the adult legs; the neurons innervating the morphologically transformed bristles follow pathways and establish connections that are appropriate for chemosensory bristles.

        Poxn was isolated as it shared a network specific domain with prd that specifies position along the antero posterior axis of the embryo.

        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 40 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DPiM - Drosophila Protein interaction map
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Synonyms and Secondary IDs (14)
        Reported As
        Symbol Synonym
        Secondary FlyBase IDs
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (187)