FlyBase Gene Report: Dmel\Pp1-13C

General Information

Symbol

Dmel\Pp1-13C

Species

D. melanogaster

Name

Protein phosphatase 1 at 13C

Annotation Symbol

CG9156

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003132

Gene Model Status

Current

Stock Availability

20 publicly available

Enzyme Name (EC)

protein-serine/threonine phosphatase (3.1.3.16)

Gene Summary

Protein phosphatase 1 at 13C (Pp1-13C) encodes a phosphatase involved in hedgehog signaling regulation and sperm competition. [Date last reviewed: 2019-08-01] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
Red Book
Interactive Fly

Also Known As

PP1α13C, PP1, PP1 13C, PP1c, 13C

Key Links

Genomic Location

Cytogenetic map

13B9-13B9

Sequence location

X:15,359,101..15,361,061 [-]

Recombination map

1-50

RefSeq locus

NC_004354 REGION:15359101..15361061

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein serine/threonine phosphatase activity

inferred from direct assay

(Dombradi et al., 1993)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables hydrolase activity

inferred from electronic annotation with InterPro:IPR004843, InterPro:IPR006186

(InterPro Project Members, 2004-)

enables protein serine/threonine phosphatase activity

inferred from biological aspect of ancestor with PANTHER:PTN000185233

(GO Reference Genome Project, 2011-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in negative regulation of BMP signaling pathway

inferred from genetic interaction with FLYBASE:flw; FB:FBgn0000711,FLYBASE:Pp1-13C; FB:FBgn0003132,FLYBASE:put; FB:FBgn0003169,FLYBASE:Pp1-87B; FB:FBgn0004103

(Bennett et al., 2003)

involved_in negative regulation of smoothened signaling pathway

inferred from genetic interaction with FLYBASE:NiPp1; FB:FBgn0026402

(Su et al., 2011)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in regulation of glycogen biosynthetic process

inferred from sequence or structural similarity with UniProtKB:P62138

(Gene Ontology Curators, 2002-)

involved_in regulation of glycogen catabolic process

inferred from sequence or structural similarity with UniProtKB:P62138

(Gene Ontology Curators, 2002-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of protein phosphatase type 1 complex

inferred from direct assay

(Kerekes et al., 2014)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000185233

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000185231

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

PPP FAMILY PROTEIN SERINE/THREONINE PHOSPHATASES

PTW-PP1 COMPLEX PP1-13C VARIANT

Pathway (FlyBase)

NEGATIVE REGULATORS OF HEDGEHOG SIGNALING PATHWAY

NEGATIVE REGULATORS OF BMP SIGNALING PATHWAY

Protein Family (UniProt)

Belongs to the PPP phosphatase family. PP-1 subfamily. (Q05547)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

protein serine/threonine phosphatase activity

(1) O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate (3.1.3.16)

(2) O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate (3.1.3.16)

Summaries

Gene Snapshot

Protein phosphatase 1 at 13C (Pp1-13C) encodes a phosphatase involved in hedgehog signaling regulation and sperm competition. [Date last reviewed: 2019-08-01]

Gene Group (FlyBase)

PPP FAMILY PROTEIN SERINE/THREONINE PHOSPHATASES -: The Phosphoprotein Phosphatase (PPP) family members are serine/threonine phosphatases. They often function in association with regulatory subunits. (Adapted from PMID:11230548 and PMID:19879837).
PTW-PP1 COMPLEX PP1-13C VARIANT -: A nuclear protein serine/threonine phosphatase complex that regulates gene expression. The core of the complex is formed by a protein phosphatase 1 catalytic subunit and the scaffolding/nuclear-targeting subunit PNUTS. (Adapted from FBrf0262508 and PMID:20516061.)

Pathway (FlyBase)

NEGATIVE REGULATORS OF HEDGEHOG SIGNALING PATHWAY -: Negative regulators of hedgehog signaling down-regulate the pathway, resulting in the repression of transcription of hh-responsive genes.
NEGATIVE REGULATORS OF BMP SIGNALING PATHWAY -: Negative regulators of Bone Morphogenetic Protein (BMP) signaling down-regulate the pathway, ultimately resulting in the decreased nuclear activity of the Mad/Med transcription factor complex.

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Pp1: Protein phosphatase 1

Encodes a protein phosphatase 1 catalytic subunit that shows a high degree of similarity to rabbit protein phosphatase 1α in its enzymatic and physiochemical characteristics and has a predicted protein sequence that is 92% identical to that of rabbit PP-1α.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Pp1-13C for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q05547)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.53

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Pp1-13C-RA

FBtr0074017

NM_080182

1892

302

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Pp1-13C-PA

FBpp0073834

34.6

302

5.47

Q05547

NP_524921

AAF48448

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with Nop17l.

(UniProt, Q05547)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q05547

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pp1-13C using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pp1-13C_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.87

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

testis | apical

(Cash and Andrews, 2012)

Additional Descriptive Data

Pp1-13C expression is highest in the apex of the testis and gradually decreases. Weak expression is observed in the germarium.

(Cash and Andrews, 2012)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010, Dorus et al., 2006)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of protein phosphatase type 1 complex

inferred from direct assay

(Kerekes et al., 2014)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Pp1-13C in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pp1-13C

View exonic expression by tissue for Dmel\Pp1-13C

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

48531/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003132

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003132

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003132

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9156

Flygut - An atlas of the Drosophila adult midgut

FBgn0003132

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pp1-13C

Transgenic constructs containing regulatory region of Pp1-13C

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 16 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^insc-Mz1407

Pp1-13C^GD14139

lethal, with Scer\GAL4^pnr-MD237

Pp1-13C^GD14139

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Pp1-13C^GD14139

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pp1-13C^GD14139

viable, with Scer\GAL4^Mef2.PR

Pp1-13C^GD14139

viable, with Scer\GAL4^tin.CΔ4

Pp1-13C^GD14139

Other Phenotypes

Allele

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

Pp1-13C^GD14139

flightless, with Scer\GAL4^Mef2.PR

Pp1-13C^GD14139

Phenotype manifest in

Allele

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

Pp1-13C^GD14139

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

Pp1-13C^GD14139

myofibril, with Scer\GAL4^Mef2.PR

Pp1-13C^GD14139

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (15)

Hsap\PPP1CA

11 of 14

Yes

Hsap\PPP1CC

11 of 14

Yes

7 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (14)

Rnor\Ppp1ca

11 of 14

Yes

10 of 14

7 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

1 of 14

Mus musculus (laboratory mouse) (14)

Mmus\Ppp1ca

11 of 14

Yes

9 of 14

7 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

Yes

Mmus\Ppp5c

2 of 14

Xenopus tropicalis (Western clawed frog) (16)

Xtro\ppp1cc

9 of 13

Yes

5 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (18)

Drer\ppp1cc

11 of 14

Yes

10 of 14

10 of 14

7 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (48)

Cele\gsp-2

10 of 14

Yes

Cele\C06A1.3

7 of 14

Yes

Cele\C25A6.1

7 of 14

Yes

Cele\C47A4.3

7 of 14

Yes

Cele\F25B3.4

7 of 14

Yes

Cele\F58G1.3

7 of 14

Yes

Cele\ZK938.1

7 of 14

Yes

Cele\C23G10.1

6 of 14

Yes

Cele\F22D6.9

6 of 14

Yes

Cele\F23B12.1

6 of 14

Yes

Cele\F52H3.6

6 of 14

Yes

Cele\gsp-1

6 of 14

Cele\pph-1

6 of 14

Yes

Cele\T25B9.2

6 of 14

Yes

Cele\Y69E1A.4

6 of 14

Yes

Cele\Y71G12B.30

6 of 14

Yes

Cele\ZK354.9

6 of 14

Yes

Cele\C09H5.7

5 of 14

Yes

Cele\C24H11.1

5 of 14

Yes

Cele\C24H11.2

5 of 14

Yes

Cele\C30A5.4

5 of 14

Yes

Cele\C34D4.2

5 of 14

Yes

Cele\F26B1.5

5 of 14

Yes

Cele\F42G8.8

5 of 14

Yes

Cele\F49E11.7

5 of 14

Yes

Cele\gsp-3

5 of 14

Yes

Cele\gsp-4

5 of 14

Yes

Cele\R13A5.11

5 of 14

Yes

Cele\T16G12.7

5 of 14

Yes

Cele\ZC477.2

5 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

Yes

Cele\R08C7.8

4 of 14

Yes

Cele\Y40H4A.2

4 of 14

Yes

Cele\C27B7.6

3 of 14

Yes

Cele\F44B9.9

3 of 14

Yes

Cele\pph-4.2

3 of 14

Cele\R03D7.8

3 of 14

Yes

Cele\W03D8.2

3 of 14

Yes

Cele\F20D6.6

2 of 14

Yes

Cele\F40E3.5

2 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (9)

Agam\AgaP_AGAP011166

9 of 12

Yes

5 of 12

5 of 12

5 of 12

4 of 12

3 of 12

3 of 12

2 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (35)

Atha\TOPP3

12 of 13

Yes

Atha\TOPP4

12 of 13

Yes

Atha\TOPP1

10 of 13

Yes

Atha\TOPP2

10 of 13

Yes

Atha\TOPP5

9 of 13

Yes

Atha\TOPP7

9 of 13

Atha\TOPP6

7 of 13

Yes

Atha\TOPP8

6 of 13

Yes

Atha\TOPP9

6 of 13

Yes

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

Yes

Atha\BSL2

3 of 13

Yes

Atha\BSL3

3 of 13

Yes

Atha\BSU1

3 of 13

Yes

Atha\PP2A

3 of 13

Atha\PP7

3 of 13

Yes

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT3G09970

1 of 13

Yes

Atha\SGT1A

1 of 13

Atha\SGT1B

1 of 13

Atha\SLP1

1 of 13

Yes

Atha\SLP2

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (13)

Scer\GLC7

11 of 13

Yes

Scer\PPZ1

5 of 13

Yes

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

2 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (11)

Spom\dis2

11 of 12

Yes

8 of 12

4 of 12

4 of 12

4 of 12

4 of 12

Yes

3 of 12

3 of 12

3 of 12

2 of 12

1 of 12

Yes

Escherichia coli (enterobacterium) (3)

Ecol\pphA

2 of 11

Yes

Ecol\pphB

2 of 11

Yes

Ecol\apaH

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Pp1-13C. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (19)

Pp1-87B

12 of 13

Pp1α-96A

12 of 13

flw

11 of 13

7 of 13

6 of 13

6 of 13

6 of 13

6 of 13

mts

5 of 13

Pp1-Y1

5 of 13

Pp4-19C

5 of 13

CG11597

4 of 13

PpV

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

Hcf

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PPP1CA; protein phosphatase 1 catalytic subunit alpha

11 of 14

176875

PPP1CC; protein phosphatase 1 catalytic subunit gamma

11 of 14

176914

PPP1CB; protein phosphatase 1 catalytic subunit beta

7 of 14

600590

NOONAN SYNDROME-LIKE DISORDER WITH LOOSE ANAGEN HAIR 2; NSLH2

Noonan syndrome-like disorder with loose anagen hair 2

PPP2CA; protein phosphatase 2 catalytic subunit alpha

4 of 14

176915

HOUGE-JANSSENS SYNDROME 3; HJS3

PPP2CB; protein phosphatase 2 catalytic subunit beta

4 of 14

176916

PPP4C; protein phosphatase 4 catalytic subunit

4 of 14

602035

PPP6C; protein phosphatase 6 catalytic subunit

4 of 14

612725

PPEF1; protein phosphatase with EF-hand domain 1

3 of 14

300109

PPEF2; protein phosphatase with EF-hand domain 2

3 of 14

602256

PPP3CA; protein phosphatase 3 catalytic subunit alpha

3 of 14

PROTEIN PHOSPHATASE 3, CATALYTIC SUBUNIT, ALPHA ISOFORM; PPP3CA

developmental and epileptic encephalopathy 91

PPP3CB; protein phosphatase 3 catalytic subunit beta

3 of 14

114106

PPP3CC; protein phosphatase 3 catalytic subunit gamma

3 of 14

114107

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Pp1-13C - ASPP

anti tag coimmunoprecipitation, anti tag western blot

(Bertran et al., 2019)

Pp1-13C - CG1532

experimental knowledge based

(Guruharsha et al., 2011)

Pp1-13C - CG18787

experimental knowledge based

(Guruharsha et al., 2011)

Pp1-13C - CG18789

experimental knowledge based

(Guruharsha et al., 2011)

Pp1-13C - CG31244

two hybrid

(Bennett et al., 2006)

Pp1-13C - CG31457

two hybrid

(Bennett et al., 2006)

Pp1-13C - CG42748

two hybrid

(Bennett et al., 2006)

Pp1-13C - CG7029

two hybrid

(Bennett et al., 2006)

Pp1-13C - CG7049

experimental knowledge based

(Guruharsha et al., 2011)

Pp1-13C - CG9933

two hybrid

(Bennett et al., 2006)

Pp1-13C - Gbs-70E

two hybrid

(Kerekes et al., 2014)

Pp1-13C - Gbs-76A

two hybrid

(Bennett et al., 2006)

Pp1-13C - I-2

experimental knowledge based, two hybrid

(Guruharsha et al., 2011, Bennett et al., 2006)

Pp1-13C - I-3

two hybrid

(Bennett et al., 2006)

Pp1-13C - NiPp1

two hybrid

(Kirchner et al., 2008, Bennett et al., 2006)

Pp1-13C - Nop17l

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Bennett et al., 2006)

Pp1-13C - PNUTS

two hybrid

(Ciurciu et al., 2013, Bennett et al., 2006)

Pp1-13C - PPP1R15

two hybrid

(Bennett et al., 2006)

Pp1-13C - Ppi1

two hybrid

(Bennett et al., 2006)

Pp1-13C - Sara

two hybrid

(Bennett et al., 2006, Bennett and Alphey, 2002)

Pp1-13C - Spindly

two hybrid

(Bennett et al., 2006)

Pp1-13C - ZAP3

two hybrid

(Bennett et al., 2006)

Pp1-13C - Zasp66

two hybrid

(Bennett et al., 2006)

Pp1-13C - corolla

two hybrid

(Bennett et al., 2006)

Pp1-13C - ec

two hybrid

(Bennett et al., 2006)

Pp1-13C - mars

two hybrid

(Bennett et al., 2006)

Pp1-13C - neb

two hybrid

(Bennett et al., 2006)

Pp1-13C - pico

two hybrid

(Bennett et al., 2006)

Pp1-13C - sds22

anti tag coimmunoprecipitation, anti tag western blot

(Grusche et al., 2009)

Pp1-13C - smo

anti tag coimmunoprecipitation, western blot

(Su et al., 2011)

Pp1-13C - tim

anti tag coimmunoprecipitation, anti tag western blot

(Fang et al., 2007)

Pp1-13C - trx

two hybrid

(Bennett et al., 2006, Rudenko et al., 2003)

Pp1-13C - uri

two hybrid

(Kirchner et al., 2008)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Acn

enhanceable

Pp1-13C

(Nandi et al., 2022)

NiPp1

suppressible

Pp1-13C

(Parker et al., 2002)

External Data

Subunit Structure (UniProtKB)

Interacts with Nop17l.

(UniProt, Q05547 )

Linkouts

BioGRID - A database of protein and genetic interactions.

71473

DroID - A comprehensive database of gene and protein interactions.

FBgn0003132

MIST (genetic) - An integrated Molecular Interaction Database

48531

MIST (protein-protein) - An integrated Molecular Interaction Database

48531

Pathways

Signaling Pathways (FlyBase)

NEGATIVE REGULATORS OF HEDGEHOG SIGNALING PATHWAY
NEGATIVE REGULATORS OF BMP SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

mRNA surveillance pathway

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Dephosphorylation of TIM

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q05547

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-50

Cytogenetic map

13B9-13B9

Sequence location

X:15,359,101..15,361,061 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

13B9-13B9

Limits computationally determined from genome sequence between P{EP}hiw^EP1308 and P{EP}Ahcy13^EP1007

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

13C-13C

(determined by in situ hybridisation)

(Dombradi et al., 1993)

Experimentally Determined Recombination Data

Location

1-50

(FlyBase, 2016)

1-50.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (20)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

39441

w¹¹¹⁸; P{GMR67E03-GAL4}attP2

52689

w¹¹¹⁸; P{GMR67E03-lexA}attP40

71118

w¹¹¹⁸; P{R67E03-p65.AD}attP40

69455

w¹¹¹⁸; P{R67E03-GAL4.DBD}attP2

89436

w¹¹¹⁸; P{R67E03-p65.AD}attP40; P{R9C12-GAL4.DBD}attP2

23701

w^*; P{UAS-Pp1-13C.HA}3/TM6C, cu¹ Sb¹

89013

w¹¹¹⁸; P{R46E03-p65.AD}attP40; P{R67E03-GAL4.DBD}attP2

89358

w¹¹¹⁸; P{R78B06-p65.AD}attP40; P{R67E03-GAL4.DBD}attP2

9156R-1

9156R-3

v107770

v29058

w¹¹¹⁸; P{GD14139}v29058

More stocks available...

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (24)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH10637

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003132

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003132

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.

(Kliman and Eyre-Walker, 1998)

Cloning and characterisation of Pp1-13C.

(Dombradi et al., 1993)

Relationship to Other Genes

Source for database merge of

Source for merge of: Pp1-13C CG9156

(Bayraktaroglu, 2000.4.13)

Additional comments

The Pp1-13C gene may have been derived from a related phosphoprotein phosphatase gene by retroposition and subsequent tandem duplication.

(Miskei et al., 2011)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

13C

(Su et al., 2011, Fang et al., 2007)

CG9156

(Ibaraki et al., 2021, Kolkhof et al., 2017, Cammarato et al., 2011, Greenspan and Clark, 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Wheeler et al., 2004, Morrison et al., 2000)

DmPp1-13C

(Cohen et al., 2005)

PP1

(Brantley and Di Talia, 2021, Patke et al., 2020, Sehgal, 2008, Fang et al., 2007, Tan et al., 2007, Lioultcheva et al., 2002, Alphey et al., 2000, Helps et al., 1998)

PP1 13C

(Morrison et al., 2000, Armstrong et al., 1998, Cohen, 1997, Dombradi et al., 1993, Chen et al., 1992)

PP1 β13c

(Grusche et al., 2009)

PP1-13C

(Xue et al., 2019, Asensio et al., 2010)

PP13C

(Cohen, 1993.1.13)

PP1c

(Rudenko et al., 2003, Rudenko et al., 2003, Bennett and Alphey, 2002, Bennett and Alphey, 2002)

PP1α-13C

(Rudenko et al., 2003)

PP1α13C

(Bertran et al., 2019, Ciurciu et al., 2013, Guruharsha et al., 2011, Kirchner et al., 2008, Kirchner et al., 2007, Swanhart et al., 2007, Bennett et al., 2006, Bennett et al., 2003, Parker et al., 2002, Bennett et al., 1999, Alphey et al., 1998)

Pp1

(Henikoff, 1996)

Pp1-13C

(Ramesh et al., 2023, Chang et al., 2022, Nandi et al., 2022, Ibaraki et al., 2021, Zhao et al., 2020, Ashton-Beaucage and Therrien, 2017, Brewer-Jensen et al., 2016, dos Santos, 2014, Kerekes et al., 2014, Cash and Andrews, 2012, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Neumüller et al., 2011, Fernández-Ayala et al., 2010, Wasbrough et al., 2010, Kirchner, 2007.7.19, Dorus et al., 2006, Wheeler et al., 2004)

pp1-13C

(Zur Lage et al., 2018, Ceriani et al., 2002)

Name Synonyms

Protein phosphatase 1 13C

(Cohen, 1993.1.13)

Protein phosphatase 1 at 13C

(Cammarato et al., 2011)

protein phosphatase 1

(Fang et al., 2007)

Secondary FlyBase IDs

FBgn0030632

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 39 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

48531

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q05547

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q05547

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q05547

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

48531/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003132

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003132

FlyMine - An integrated database for Drosophila genomics

FBgn0003132

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9156

MARRVEL_MODEL - MARRVEL (model organism gene)

48531

Linkouts

BioGRID - A database of protein and genetic interactions.

71473

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003132

DroID - A comprehensive database of gene and protein interactions.

FBgn0003132

DRSC - Results frm RNAi screens

FBgn0003132

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pp1-13C_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003132

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003132

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9156

Flygut - An atlas of the Drosophila adult midgut

FBgn0003132

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

mRNA surveillance pathway

MIST (genetic) - An integrated Molecular Interaction Database

48531

MIST (protein-protein) - An integrated Molecular Interaction Database

48531

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Dephosphorylation of TIM

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q05547

References (103)

The following external sites may use different assemblies or annotations than FlyBase.

3.87

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