FlyBase Gene Report: Dmel\Ppn

ADAM WITH THROMBOSPONDIN MOTIF -: A Disintegrin And Metalloprotease Thrombospondin motif (ADAMTS) belongs to the metzincin superfamily of metalloproteases. ADAMTS is related to ADAM proteins sharing in part their domain organisation. However, ADAMTS includes proteins that contain a variable number of thrombospondin-like repeats instead of disintegrin and cysteine rich domains. (Adapted from FBrf0214506).

Protein Function (UniProtKB)

Essential extracellular matrix (ECM) protein that influences cell rearrangements. May act by modulating metalloproteinases action during organogenesis. Able to non-competitively inhibit procollagen N-proteinase, an ADAMTS metalloproteinase.

(UniProt, Q868Z9)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Ppn or the JBrowse view of Dmel\Ppn for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ppn-RC

FBtr0300337

NM_001170289

7372

2174

Ppn-RE

FBtr0301837

NM_176575

9544

2898

Ppn-RF

FBtr0301838

NM_176574

9178

2776

Ppn-RG

FBtr0301839

NM_001170290

9373

2841

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Ppn-PC

FBpp0289566

231.8

2174

4.10

NP_001163760

ACZ95054

Ppn-PE

FBpp0291051

313.0

2898

4.19

NP_788752

AAF56795

Ppn-PF

FBpp0291052

299.5

2776

4.18

NP_788751

AAF56794

Ppn-PG

FBpp0291053

306.6

2841

4.17

NP_001163761

ACZ95055

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Homooligomer; disulfide-linked.

(UniProt, Q868Z9)

Post Translational Modification

N-glycosylated. Sulfated.

(UniProt, Q868Z9)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ppn using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ppn_1

Linkouts

Gene Ontology (5 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

extracellular matrix structural constituent

inferred from mutant phenotype

(assigned by UniProt )

(Kramerova et al., 2000)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

peptidase activity

inferred from electronic annotation with InterPro:IPR010909

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

serine-type endopeptidase inhibitor activity

inferred from electronic annotation with InterPro:IPR002223

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

extracellular matrix organization

inferred from mutant phenotype

(assigned by UniProt )

(Kramerova et al., 2000)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

basement membrane

inferred from direct assay

(Campbell et al., 1987)

inferred from direct assay

(assigned by UniProt )

(Kramerova et al., 2000)

Terms Based on Predictions or Assertions (0 terms)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 8

cephalic furrow

Comment: probe does not distinguish isoforms

(Kramerova et al., 2003)

ventral furrow

Comment: probe does not distinguish isoforms

(Kramerova et al., 2003)

amnioproctodeal invagination

Comment: probe does not distinguish isoforms

(Kramerova et al., 2003)

embryonic stage 10

mesoderm | segmentally repeated

Comment: probe does not distinguish isoforms

(Kramerova et al., 2003)

embryonic stage 12 -- 14

hemocyte

Comment: NOT Ppn-RE

(Kramerova et al., 2003)

embryonic stage 14 -- 15

dorsal vessel primordium