protein is expressed in male accessory glands. It is initially expressed at high levels in all accessory gland cells, but protein levels decline with increasing age of virgin males. prd
protein levels decline more rapidly in the main cells than in the secondary cells. In 10 day males, prd
protein is detected only in scattered secondary cells in the distal region of the glands. Mating increases prd
s in both main and secondary cells throughout the glands.
protein is first detected in the anterior region of very early embryos and is subsequently restricted to a very narrow anterior stripe. During cellularization, bell-shaped stripes (stripes 3-7) are observed. The order of stripe appearance is 4 and 7, followed by 3 and 6, and finally stripe 5 emerges. At this point the anterior stripe splits into two. By mid-cellularization prd
protein has reached equal levels in all stripes. At the same time, the preferential accumulation of prd
protein at the posterior margins generates a gradient within each stripe. During the second half of cellularization, expression also occurs in a patch of cells at the anterior dorsal end of the embryo and in an eighth stripe. By the end of gastrulation, the number of stripes has doubled to 14 as a result of reduction of protein in the middle of the stripes accompanied by an increase of protein in the anterior region of the stripes. At the end of the germ band extended stage, the expression in the stripes has disappeared. prd
protein is expressed in the head region, most strongly in the maxillary lobe, but also in the labial and mandibular lobes and in the clypeolabrum. prd
protein is also detected in the developing CNS in 2-3 specific neurons per hemisegment.