Atr-II, TGF-β, l(3)10460
receptor for Dpp - type II TGFß receptor - functions in both Dpp/BMP and Activin signaling - mutants lack and expression in the visceral mesoderm and fail to induce in the adjacent endodermal cells - pathway specificity in signaling output is determined by which type I receptor (Dpp/BMP or Activin one) is engaged in the complex with Put
Gene model reviewed during 5.44
Stop-codon suppression (UAG) postulated; FBrf0216885.
gene_with_stop_codon_read_through ; SO:0000697
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\put using the Feature Mapper tool.
put transcripts are detected throughout development on northern blots. They are abundant in nurse cells and in the developing oocyte. The transcript persists at high levels in the embryo until cellularization. Transcript levels decrease at cellularization but remain highest under the pole cells. At the start of gastrulation, transcripts are observed in the invaginating mesoderm. By stage 11, expression is observed along the entire length of the germ band in the mesoderm as well as in the developing gut. Expression continues in the fore- mid- and hindgut throughout most of embryogenesis. Third instar larval imaginal discs show prominant put expression.
put protein is observed throughout the embryo at syncytial blastoderm stage. At the start of gastrulation, Pburs protein is observed in the invaginating mesoderm. By stage 11 it is expressed along the entire length of the germ band in the mesoderm as well as in the developing gut. Expression continues in the fore- mid- and hindgut throughout most of embryogenesis.
GBrowse - Visual display of RNA-Seq signalsView Dmel\put in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: put CG7904
put is required during the late larval stage for development of adult-specific neurons.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
One of five genes identified as encoding downstream components of the dpp signalling cascade which is necessary for blocking salivary gland gene activation by Scr in the dorsal region of parasegment 2.
shn and put are required to limit transient amplification of germ cells. Mosaic analysis demonstrates shn and put act within somatic cyst cells that surround germ cells, rather than in germ cells. Thus a cyst-cell-derived signal restricts germ cell proliferation and this signal is initiated by input from a member of the TGF-β superfamily. Thus, a signal relay regulates progression through the germline stem cell lineage.
dpp receptors tkv and put are not required for photoreceptor cell differentiation. tkv and put play a nonessential role in morphogenetic furrow progression, but are required for initiation of the furrow at the posterior margin. Ectopic activation of the dpp pathway does not lead to ectopic neuronal differentiation.
dpp receptors put and tkv, or the shn transcription factor, are autonomously required for cell proliferation in the entire developing wing. The dpp signal has to travel several cell diameters from its source in order to reach all cells that require its signal.
Activin type II receptor is encoded by the put gene. Loss of the maternal function of the put gene disrupts a signalling pathway required for embryonic pattern function, causing cells throughout the embryo to adopt a fate normally reserved for those at the ventral surface.
Mitotic recombination analysis demonstrates a requirement for maternal put gene product in dorsal-ventral patterning.
Ligand binding studies reveal put gene product binds activin with high specificity. The sequence and binding characteristics of put indicate that only a small number of amino acids are required for specification of activin binding.
TGF-β like activity is present in cell extracts.