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General Information
Symbol
Dmel\ras
Species
D. melanogaster
Name
raspberry
Annotation Symbol
CG1799
Feature Type
FlyBase ID
FBgn0003204
Gene Model Status
Stock Availability
Enzyme Name (EC)
IMP dehydrogenase (1.1.1.205)
Gene Snapshot
In progress.Contributions welcome.
Also Known As
l(1)G0127, l(1)G0056, IMPDH, l(1)G0436, l(1)G0098
Key Links
Genomic Location
Cytogenetic map
Sequence location
X:10,744,502..10,749,097 [+]
Recombination map
1-32
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the IMPDH/GMPR family. (Q07152)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH (1.1.1.205)
Summaries
Gene Group (FlyBase)
OTHER CH-OH OXIDOREDUCTASES, NAD OR NADP AS ACCEPTOR -
The Other CH-OH oxidoreductases, NAD or NADP as acceptor group, is a collection of oxidoreductases that do not fit into any of the other major CH-OH oxidoreductases, NAD or NADP as acceptor group.
Protein Function (UniProtKB)
Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.
(UniProt, Q07152)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
ras: raspberry
Defective in pteridine synthesis; eye color dark ruby. Appears to affect the level of guanosine triphosphate cyclohydrolase activity; ras alleles exhibit increased activity compared to wild type at pupariation, but decreased activity at eclosion (Evans and Howell, 1979, Biochem. Genet. 16: 13-26). Not a purine auxotroph. Color autonomous in larval optic disks transplanted into wild-type hosts (Beadle and Ephrussi, 1936, Genetics 21: 230). ras/rasl display ras eye color. Larval Malpighian tubes nearly wild type; not useful for classification (Brehme and Demerec, 1942, Growth 6: 351-56). ras is but one of four types of mutations belonging to an interrelated complex involved some way in purine metabolism: ras, a nonauxotrophic eye-color mutant; two purine auxotrophs, pur1 and gua1, which complement ras and one another; and rasl, lethal alleles that fail to complement completely the other three types. The complex shares genetic features with rudimentary.
Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\ras or the JBrowse view of Dmel\ras for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.51
Gene model reviewed during 5.45
Gene model reviewed during 5.56
gene_with_stop_codon_read_through ; SO:0000697
Stop-codon suppression (UAA) postulated; FBrf0234051.
Gene model reviewed during 6.25
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0071495
2519
537
FBtr0071494
2321
537
FBtr0332294
2810
537
FBtr0343043
2415
537
FBtr0474163
2415
590
Additional Transcript Data and Comments
Reported size (kB)
2.5, 1.9 (northern blot)
2.4 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0071424
57.8
537
7.49
FBpp0071423
57.8
537
7.49
FBpp0304573
57.8
537
7.49
FBpp0309791
57.8
537
7.49
FBpp0423162
63.8
590
8.02
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

537 aa isoforms: ras-PA, ras-PC, ras-PD, ras-PE
Additional Polypeptide Data and Comments
Reported size (kDa)
537 (aa); 58 (kD predicted)
Comments
Bacterially expressed ras protein has inosine monophosphate dehydrogenase activity.
External Data
Subunit Structure (UniProtKB)
Homotetramer.
(UniProt, Q07152)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ras using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (6 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity with MGI:MGI:96567
inferred from biological aspect of ancestor with PANTHER:PTN000226903
(assigned by GO_Central )
Biological Process (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000226903
(assigned by GO_Central )
inferred from sequence or structural similarity with MGI:MGI:96567
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000226902
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
dissected tissue
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\ras in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 80 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of ras
Transgenic constructs containing regulatory region of ras
Deletions and Duplications ( 83 )
Disrupted in
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
photoreceptor cell & axon
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
12 of 15
Yes
No
2 of 15
No
Yes
2 of 15
Yes
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
13 of 15
Yes
Yes
2 of 15
No
Yes
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (4)
12 of 13
Yes
Yes
12 of 13
Yes
Yes
2 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (4)
8 of 12
Yes
Yes
7 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (5)
14 of 15
Yes
Yes
14 of 15
Yes
Yes
13 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
11 of 15
Yes
Yes
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (4)
8 of 9
Yes
Yes
8 of 9
Yes
Yes
1 of 9
No
Yes
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (3)
14 of 15
Yes
Yes
12 of 15
No
Yes
11 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
11 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919064R )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502YF )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W045F )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X035Y )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0EAV )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 2 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Homotetramer.
    (UniProt, Q07152 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    X
    Recombination map
    1-32
    Cytogenetic map
    Sequence location
    X:10,744,502..10,749,097 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    9E1-9E2
    Limits computationally determined from genome sequence between P{EP}rasEP1235&P{EP}rasEP1427 and P{EP}EP1645
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    9E3-9E8
    (determined by in situ hybridisation) 9E1--2 (determined by in situ hybridisation) 9E3--4 (determined by in situ hybridisation) 9E1--4 (determined by in situ hybridisation) 9E2--4 (determined by in situ hybridisation)
    9E1-9E4
    (determined by in situ hybridisation)
    9E1-9E4
    (determined by in situ hybridisation) 9E3--4 (determined by in situ hybridisation) 9E1--2 (determined by in situ hybridisation)
    9E-9E
    (determined by in situ hybridisation) 9E1--2 (determined by in situ hybridisation)
    9E1-9E2
    (determined by in situ hybridisation) 9E3--4 (determined by in situ hybridisation)
    9E3-9E4
    (determined by in situ hybridisation)
    There is a discrepancy between the in situ localisation (7E3--7E6) and the molecular localisation based on the flanking sequence (9E2--9E4) for the "l(1)G0238" (rasG0238) line. The available data suggests that it is most likely that the molecular localisation (9E2--9E4) is correct and that the in situs were misinterpreted.
    Experimentally Determined Recombination Data
    Left of (cM)
    Notes
    Stocks and Reagents
    Stocks (66)
    Genomic Clones (15)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (67)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of
      Source for merge of: ras CG11485 CG1799
      Source for merge of: ras l(1)G0388 l(1)G0391 l(1)G0002 l(1)G0056 l(1)G0351 l(1)G0482 l(1)G0098 l(1)G0127 l(1)G0436 l(1)G0238
      Additional comments
      Other Comments
      S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
      Inosine monophosphate dehydrogenase (IMPD) is the product of the ras locus.
      Mutant analysis suggests that GMP synthesis from IMP is an essential process, and that activity of the ras gene product, IMPDH, is regulated in a tissue-specific manner.
      ras is but one of four types of mutations belonging to an interrelated complex involved some way in purine metabolism: ras, a nonauxotrophic eye-color mutant; two purine auxotrophs, pur1 and gua1, which complement ras and one another; and ras lethal alleles that fail to complement completely the other three types. The complex shares genetic features with r.
      Defective in pteridine synthesis; eye color dark ruby. Appears to affect the level of guanosine triphosphate cyclohydrolase activity; ras alleles exhibit increased activity compared to wild type at pupariation, but decreased activity at eclosion (FBrf0031059). Not a purine auxotroph. Color autonomous in larval optic discs transplanted into wild-type hosts (FBrf0003530). ras1/ras-lethal display ras eye color. Larval Malpighian tubes nearly wildtype; not useful for classification FBrf0005752).
      The different temperature sensitive periods for the ras gene product, demonstrated with the rasl1 mutation, indicate tissue-specific activation of a gene at different times during development, although the possibility of activation of preformed polypeptides has not been eliminated.
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 87 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      KEGG Genes - Molecular building blocks of life in the genomic space.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      modMine - A data warehouse for the modENCODE project
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyMine - An integrated database for Drosophila genomics
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Synonyms and Secondary IDs (28)
      Reported As
      Name Synonyms
      IMP dehydrogenase
      inosine monophosphate dehydrogenase
      lethal (1) G0380
      raspberry-lethal
      Secondary FlyBase IDs
      • FBgn0026670
      • FBgn0026696
      • FBgn0028270
      • FBgn0028295
      • FBgn0028297
      • FBgn0028302
      • FBgn0028318
      • FBgn0028340
      • FBgn0028363
      • FBgn0029044
      • FBgn0030231
      • FBgn0030232
      • FBgn0040205
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (152)