General Information
Symbol
Dmel\rec
Species
D. melanogaster
Name
recombination-defective
Annotation Symbol
CG31293
Feature Type
FlyBase ID
FBgn0003227
Gene Model Status
Stock Availability
Enzyme Name (EC)
Adenosinetriphosphatase (3.6.1.3)
Gene Snapshot
Recombination-defective interacts with other Mini-Chromosome Maintenance (MCM)-related proteins (mei-217 and mei-218) to make meiotic crossovers. [Date last reviewed: 2016-06-23]
Also Known As
MCM8
Genomic Location
Cytogenetic map
Sequence location
3R:15,823,019..15,832,632 [-]
Recombination map
3-58
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
Belongs to the MCM family. (Q9VF30)
Molecular Function (see GO section for details)
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + H(2)O = ADP + phosphate (3.6.1.3)
Summaries
UniProt Contributed Function Data
Required for meiotic DNA recombination in females. Probably not involved in DNA repair and recombination in somatic cells.
(UniProt, Q9VF30)
Phenotypic Description from the Red Book (Lindsley and Zimm 1992)
rec: recombination defective (R.F. Grell)
Recombination in homozygous females drastically reduced; total cross over between y and f in the X chromosome reduced from 56% to 2% with distal recombination being more severely affected than proximal. X nondisjunction increased from virtually 0 to 27%. In rec1/rec2, females show a similar reduction for ten regions between al and sp, where crossing over decreases to 9.1%, corresponding to 9% of normal. The number of progeny per homozygous female is reduced 75%; attributed to lethality of nondisjunctional products arising from distributive pairing between noncrossover heterologues. Phenotype of rec/+ is the same as wild-type; rec1 and rec2 homozygotes and hemizygotes have equivalent effects on recombination.
rec3
At 17 recombination in rec3/+, rec3/rec3, and rec3/Df(3R)sbd105 is elevated. At 25, the homozygote and the heterozygote display normal values, but recombination in the hemizygote is significantly reduced. At 31, rec3/+ remains normal but rec3/rec3 recombination is drastically reduced. The hemizygote is sterile above 28. In rec2/rec3 females which show the most extreme temperature responses, application of the restrictive temperature at sequential days during development at 25 shows control value activity to be reduced only with treatment on days six and seven, and more precisely during a 36-hour period beginning at the time of pro-oocyte formation between 126 and 132 hours and terminating at 162 hours. This 36-hour period coincides with the boundaries of the S period as well as those of the heat-sensitive period for enhancing recombination in the normal genotype [Grell and Day, 1974, Mechanisms in Recombination (R. F. Grell, ed.). Plenum Press, New York and London, pp. 327-49]. Activity of the rec gene product in the range of 17 to 31 shows a sharp decline between 28 and 31 typical of a protein denaturation curve. If denaturation of a rec protein by the restrictive temperature marks its active phase, then recombination must terminate at the end of S, when the restrictive temperature becomes ineffective. Electron microscopy of serially sectioned oocyte nuclei from rec2/rec3 females maintained at the restrictive or at the permissive temperature reveals synaptonemal complexes indistinguishable in length and fine structure, implicating recombination and not synapsis as the target of the rec gene product.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\rec or the JBrowse view of Dmel\rec for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.45
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0083193
4131
885
FBtr0334595
2872
885
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0082647
97.6
885
7.68
FBpp0306662
97.6
885
7.68
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

885 aa isoforms: rec-PA, rec-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\rec using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (8 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR001208
(assigned by InterPro )
traceable author statement
(assigned by UniProt )
traceable author statement
(assigned by UniProt )
inferred from electronic annotation with InterPro:IPR001208
(assigned by InterPro )
traceable author statement
(assigned by UniProt )
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR001208
(assigned by InterPro )
traceable author statement
(assigned by UniProt )
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
traceable author statement
(assigned by UniProt )
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\rec in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 10 )
For All Classical and Insertion Alleles Show
 
Allele of rec
Class
Mutagen
Associated Insertion
Stocks
Known lesion
Other relevant insertions
insertion of enhancer trap binary system
Name
Expression Data
Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of rec
Allele of rec
Mutagen
Associated Transgenic Construct
Stocks
Transgenic constructs containing regulatory region of rec
Deletions and Duplications ( 19 )
Summary of Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
Yes
2 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
Yes
2 of 15
No
Yes
1 of 15
No
No
Rattus norvegicus (Norway rat) (3)
8 of 13
Yes
Yes
2 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (3)
6 of 12
Yes
Yes
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (4)
9 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
1 of 15
Yes
No
1 of 15
Yes
No
Arabidopsis thaliana (thale-cress) (1)
3 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
1 of 15
Yes
No
1 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (0)
No orthologs reported.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091904DX )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091505HE )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05DV )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X026F )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Tetranychus urticae
Two-spotted spider mite
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Human Disease Model Data
FlyBase Human Disease Model Reports
    Alleles Reported to Model Human Disease (Disease Ontology)
    Download
    Models ( 0 )
    Allele
    Disease
    Evidence
    References
    Interactions ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments ( 0 )
     
    Human Orthologs (via DIOPT v7.1)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please look at the Interaction Group reports for full details of the physical interactions
    protein-protein
    Interacting group
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Pathways
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-58
    Cytogenetic map
    Sequence location
    3R:15,823,019..15,832,632 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    89A5-89A5
    Limits computationally determined from genome sequence between P{PZ}l(3)0653606536 and P{PZ}blp01618
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location
    3-57.0--58.2
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (9)
    Genomic Clones (18)
    cDNA Clones (4)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    polyclonal
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of
    Source for merge of: rec CG4828
    Source for merge of: rec CG14875
    Additional comments
    Annotations CG14875 and CG4828 merged as CG31293 in release 3 of the genome annotation.
    Not allelic to c(3)G, since rec and c(3)G fully complement, also unlike c(3)G, rec has no discernible effect on the synaptonemal complex.
    Other Comments
    Recombination in homozygous females drastically reduced; total cross over between y and f in the X chromosome reduced from 56% to 2% with distal recombination being more severely affected than proximal. X nondisjunction increased from virtually 0 to 27%. In rec1/rec2, females show a similar reduction for ten regions between al and sp, where crossing over decreases to 9.1%, corresponding to 9% of normal. The number of progeny per homozygous female is reduced 75%; attributed to lethality of nondisjunctional products arising from distributive pairing between noncrossover heterologues. Phenotype of rec/+ is the same as wild-type; rec1 and rec2 homozygotes and hemizygotes have equivalent effects on recombination.
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 64 )
    Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    FlyMine - An integrated database for Drosophila genomics
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Synonyms and Secondary IDs (10)
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (59)