Gene Dmel\ref(2)P

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General Information

Symbol

Dmel\ref(2)P

Species

D. melanogaster

Name

refractory to sigma P

Annotation symbol

CG10360

Feature type

protein_coding_gene

FlyBase ID

FBgn0003231

Gene Model Status

Current

Stock availability

7 publicly available

Also Known As

Ref2P, p62, ref

Genomic Location

Chromosome (arm)

Recombination map

2-54.0

Cytogenetic map

37F1-37F1

Sequence location

2L:19,542,446..19,545,548 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene refractory to sigma P is referred to in FlyBase by the symbol Dmel\ref(2)P (CG10360, FBgn0003231). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: zinc ion binding. It is reported to be involved in the biological process: viral infectious cycle. 52 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Phox/Bem1p; UBA-like; Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote; Zinc finger, ZZ-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-06 and 18-24 hour embryonic stages, during late larval stages, during early pupal stages, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval central nervous system, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1627212_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of ref(2)P. Gene sequence location is 2L:19542446..19545548.

User Contributed Data

Beta version, feedback welcome

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

ref(2)P

ref(2)Pp is the first refractaire mutant found and the most thoroughly studied. Allele frequency about 0.2 in Paris population and approaches the same incidence in caged populations with or without the presence of sigma virus (Fleuriet, 1980, Genetics 95: 459-65; 1981, Genetics 97: 415-25). Refractaire phenotype expressed in cultured cells (Richard-Molard, 1975, Arch. Virol. 47: 139-46) and in imaginal disks transplanted into stably infected hosts (Bernard, 1968, Exp. Cell Res. 50: 117-26). Inhibition of viral multiplication increases with increased doses of ref(2)Pp, with added doses of ref(2)Po having an antagonistic effect (Nakamura, 1978, Mol. Gen. Genet. 159: 285-92). Strains of sigma virus capable of multiplication in restrictive strains can be selected; P+ virus strains multiply in ref(2)Pp hosts, whereas P-strains do not; no such distinction in ref(2)Po hosts. Also 5-FU-induced host-adapted strains of virus (haP) capable of replication in ref(2)Pp; many are temperature sensitive, suggesting that ref(2)Pp interacts with a viral protein (Coulon and Contamine, 1982, Virology 123: 381-92). Males homozygous for null alleles sterile; elongating spermatids display degeneration of axonemes (Dezelee, Bras, Contamine, Lopez-Ferber, Segretain, and Teninges, 1989, EMBO J. 8: 3437-46).

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

37F1-37F1

Limits computationally determined from genome sequence between P{EP}dnt^EP2158 and P{EP}EP623&P{PZ}spi⁰¹⁰⁶⁸

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

37E-37F

(determined by in situ hybridisation)

(Contamine et al., 1989)

Experimentally Determined Recombination Data

Location

2-54

(Gay, 1972)

(Laboratoire de Genetique Formelle du C.N.R.S., 1953)

2-54.0

Left of (cM)

(Laboratoire de Genetique Formelle du C.N.R.S., 1953)

Right of (cM)

(Laboratoire de Genetique Formelle du C.N.R.S., 1953)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\ref(2)P for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

ref(2)P-RA

FBtr0081253

NM_057352

2417

599

ref(2)P-RB

FBtr0100289

NM_001014491

2223

599

Additional Transcript Data & Comments

Reported size (kB)

2.4, 2.3 (unknown)

(Contamine et al., 1989)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

ref(2)P-PA

FBpp0080794

65.3

599

4.63

NP_476700

AAF53824

ref(2)P-PB

FBpp0099683

65.3

599

4.63

NP_001014491

AAX52671

Additional Polypeptide Data & Comments

Reported size (kDa)

115-125 (kD observed); 76 (kD predicted)

(Dezelee et al., 1989)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote (IPR015940)

Phox/Bem1p (IPR000270)

Zinc finger, ZZ-type (IPR000433)

UBA-like (IPR009060)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P14199

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

(Fisher et al., 2012)

Comment:maternally deposited

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

pole cell

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

(Fisher et al., 2012)