FlyBase Gene Report: Dmel\rk

General Information

Symbol

Dmel\rk

Species

D. melanogaster

Name

rickets

Annotation Symbol

CG8930

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003255

Gene Model Status

Current

Stock Availability

22 publicly available

Gene Snapshot

rickets (rk) encodes a G-protein coupled receptor that signals intracellularly using the second messenger cAMP. It binds the heterodimeric hormone bursicon and is developmentally important for molting-related behaviors. It is also required after adult emergence for cuticle hardening and melanization. [Date last reviewed: 2019-03-14]

Other Summaries

Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

DLGR2, DLGR-2, LGR2, BG:DS00180.13

Key Links

Genomic Location

Cytogenetic map

34E5-34E6

Sequence location

2L:13,982,743..14,001,248 [-]

Recombination map

2-50

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Gene Group (FlyBase)

CLASS A GPCR NEUROPEPTIDE AND PROTEIN HORMONE RECEPTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

neuropeptide receptor activity

Predictions / Assertions

G protein-coupled peptide receptor activity; G protein-coupled receptor activity; neuropeptide receptor activity; protein-hormone receptor activity

Summaries

Gene Group (FlyBase)

CLASS A GPCR NEUROPEPTIDE AND PROTEIN HORMONE RECEPTORS -: The majority of neuropeptide/protein hormone GPCR belong to the Class A family of GPCRs. The secreted ligands for this subfamily include neuropeptides, peptide hormones and protein hormones. (Adapted from FBrf0195077).

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

rk: rickets (M. Ashburner)

Strong alleles have legs, especially metathoracic ones, flattened and bent. Femora and tibiae bowed in middle; first two tarsal joints shortened, bent, and flattened; last three tarsal joints almost fused. Wings unexpanded, resembling those of pu. Posterior scutellar bristles erect and crossed. Viability may be reduced. Weak alleles do not show the leg phenotype, or they overlap wild type; may have partially expanded wings and normal posterior scutellar bristles. Even strong alleles more extreme when hemizygous with rk deficiencies.

rk4

Wings unexpanded, spread, and drooping. Posterior legs malformed. Both sexes fully viable and fertile. rk4 male mates with wild-type female only if wings removed from female. Viability 60% of wild type.

rk5

Wings sometimes fully expanded and held out.

rk6

Legs weak, wings unexpanded. Viability higher at higher temperature.

rkcyl

Abdomen cylindrical; terminal segments thickened; halteres small and melanotic; legs like those of bal, but less deformed. Viability low but fertility good.

Summary (Interactive Fly)

G-protein coupled receptor expressed in the CNS and muscle - required for wing expansion, cuticle hardening and melanization

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\rk or the JBrowse view of Dmel\rk for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

rk-RA

FBtr0080610

NM_057354

5386

1360

Additional Transcript Data and Comments

Reported size (kB)

5.5 (northern blot)

(Eriksen et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

rk-PA

FBpp0080183

150.8

1360

8.52

NP_476702

AAF53367

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

1360 (aa)

(Eriksen et al., 2000)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\rk using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

rk_1

Linkouts

Gene Ontology (16 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

neuropeptide receptor activity

inferred from physical interaction with FLYBASE:Burs; FB:FBgn0038901, FLYBASE:Pburs; FB:FBgn0264810

(Luo et al., 2005)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

G protein-coupled peptide receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN001224574

(assigned by GO_Central )

(Gaudet et al., 2011)

G protein-coupled receptor activity

inferred from sequence or structural similarity

(Radford et al., 2002)

neuropeptide receptor activity

inferred from sequence model

(Hewes and Taghert, 2001)

protein-hormone receptor activity

inferred from electronic annotation with InterPro:IPR002131

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Brody and Cravchik, 2000)

Biological Process (10 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

border follicle cell delamination

inferred from mutant phenotype

(Anllo and Schüpbach, 2016)

border follicle cell migration

inferred from mutant phenotype

(Anllo and Schüpbach, 2016)

epithelial to mesenchymal transition

inferred from mutant phenotype

(Natzle et al., 2008)

intestinal stem cell homeostasis

inferred from mutant phenotype

(Scopelliti et al., 2014)

neuropeptide signaling pathway

inferred from direct assay

(Luo et al., 2005)

regulation of chitin-based cuticle tanning

inferred from mutant phenotype

(Baker and Truman, 2002)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

activation of adenylate cyclase activity

inferred from biological aspect of ancestor with PANTHER:PTN001224574

(assigned by GO_Central )

(Gaudet et al., 2011)

adenylate cyclase-activating G protein-coupled receptor signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN001224574

(assigned by GO_Central )

(Gaudet et al., 2011)

G protein-coupled receptor signaling pathway

inferred from sequence model

(Hewes and Taghert, 2001)

inferred from sequence or structural similarity

(Brody and Cravchik, 2000)

hormone-mediated signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN001224574

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

integral component of plasma membrane

inferred from direct assay

(Luo et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

integral component of membrane

inferred from sequence or structural similarity

(Brody and Cravchik, 2000)

inferred from sequence model

(Hewes and Taghert, 2001)

integral component of plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN001224574

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Eriksen et al., 2000)

pupal stage

(Eriksen et al., 2000)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult midgut muscle

(Scopelliti et al., 2014)

Additional Descriptive Data

rk transcripts are detected in pupae and in embryos, but not in larvae or adults.

(Eriksen et al., 2000)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

integral component of plasma membrane

inferred from direct assay

(Luo et al., 2005)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\rk in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\rk

View exonic expression by tissue for Dmel\rk

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003255

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0003255

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8930-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8930

Flygut - An atlas of the Drosophila adult midgut

FBgn0003255

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 31 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of rk

Transgenic constructs containing regulatory region of rk

Deletions and Duplications ( 137 )

Disrupted in

Df(2L)64j

(Eriksen et al., 2000, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)A47

(Fossett et al., 1994, Gubb et al., 1990, Ashburner et al., 1982)

Df(2L)A376

(Baker and Truman, 2002, Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)Adh-nBR101

(Fossett et al., 1994)

Df(2L)Adh-nBR130

(Fossett et al., 1994)

Df(2L)b75

(Gubb et al., 1990, Woodruff and Ashburner, 1979)

Df(2L)b77c

(Roote, 1995.3.27)

Df(2L)b79b8

(Roote, 1995.3.27)

Df(2L)b80e3

(Eriksen et al., 2000, Ashburner et al., 1981)

Df(2L)b85f1A

(Roote, 1995.3.27)

Df(2L)b85f1

(Alexandrova et al., 1996)

Df(2L)b85f2

(Alexandrova et al., 1996)

Df(2L)b87e25

(Eriksen et al., 2000)

Df(2L)b91l5

(Roote, 2000.7.3)

Df(2L)b-L

(Baker and Truman, 2002, Ashburner et al., 1981)

Df(2L)BSC252

(Loveall and Deitcher, 2010, Christensen and Cook, 2007.3.22)

Df(2L)BSC253

(Christensen and Cook, 2007.3.22)

Df(2L)el80f1

(Eriksen et al., 2000)

Df(2L)fn7

(Eriksen et al., 2000, Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn12

(Roote, 1996.1.11, Roote, 1996.8, Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn26

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn30

(Eriksen et al., 2000, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn31

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)GT5

(Gubb et al., 1990)

Df(2L)noc11

(Roote, 1996.1.11)

Df(2L)rkw11P^LPZ07130^R-mr1

(Roote, 2000.6.13)

Df(2L)rkw11P^LPZ07130^R-mr2

(Roote, 2000.6.13)

Df(2L)rkw11P^LPZ07130^R-mr3

(Roote, 2000.6.13)

Df(2L)rkw11P^LPZ07130^R-mr4

(Roote, 2000.6.13)

Df(2L)rkw11P^LPZ07130^R-mr5

(Roote, 2000.6.13)

Df(2L)rkw11P^LPZ07130^R-mr6

(Roote, 2000.6.13)

Df(2L)rkw11P^LPZ07130^R-mr7

(Roote, 2000.6.13)

Df(2L)rkw11P^LPZ07130^R-mr8

(Roote, 2000.6.13)

Df(2L)rkw11P^LPZ07130^R-mr9

(Roote, 2000.6.13)

Df(2L)rkw11P^LPZ07130^R-mr10

(Roote, 2000.6.13)

Df(2L)rkw11P^LPZ07130^R-mr11

(Roote, 2000.6.13)

Df(2L)rkw11P^LPZ07130^R-mr12

(Roote, 2000.6.13)

Df(2L)rkw11P^LPZ07130^R-mr13

(Roote, 2000.6.13)

Df(2L)rkw11P^LPZ07130^R-mr14

(Roote, 2000.6.13)

Df(2L)rkw11P^LPZ07130^R-mr15

(Roote, 2000.6.13)

Df(2L)rkw11P^LPZ07130^R-mr16

(Roote, 2000.6.13)

Df(2L)rN149-rv3

(Roote, 2000.6.13)

Df(2L)TE35B-5

(Gubb et al., 1985)

Df(2L)TE35B-8

(Gubb et al., 1985)

Df(2L)TE35BC-6

(Gubb et al., 1984)

Df(2L)TE35BC-23

(Gubb et al., 1984)

In(2LR)D20^Lnoc^4R

(Roote et al., 1996)

Tp(2;Y)b88b15

(Roote, 1995.3.27)

Ts(YSt;2Lt)G74+Ts(YSt;2Rt)A80

(Woodruff and Ashburner, 1979)

Not disrupted in

Df(2L)A48

(Eriksen et al., 2000, Fossett et al., 1994, Gubb et al., 1990, Ashburner et al., 1982)

Df(2L)A63

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A72

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A178

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A215

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A217

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A220

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A245

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A246

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A260

(Ashburner et al., 1982)

Df(2L)A263

(Eriksen et al., 2000, Ashburner et al., 1982)

Df(2L)A264

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A266

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A267

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A337

(Fossett et al., 1994)

Df(2L)A377

(Ashburner et al., 1982)

Df(2L)A379

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A400

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A445

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)Adh-nBR100

(Fossett et al., 1994)

Df(2L)Adh-nBR102

(Fossett et al., 1994)

Df(2L)Adh-nBR103

(Fossett et al., 1994)

Df(2L)Adh-nBR105

(Fossett et al., 1994)

Df(2L)Adh-nBR106

(Fossett et al., 1994)

Df(2L)Adh-nBR107

(Fossett et al., 1994)

Df(2L)Adh-nBR117

(Fossett et al., 1994)

Df(2L)Adh-nBR118

(Fossett et al., 1994)

Df(2L)Adh-nBR119

(Fossett et al., 1994)

Df(2L)Adh-nBR120

(Fossett et al., 1994)

Df(2L)Adh-nBR121

(Fossett et al., 1994)

Df(2L)Adh-nBR122

(Fossett et al., 1994)

Df(2L)Adh-nBR125

(Fossett et al., 1994)

Df(2L)Adh-nBR126

(Fossett et al., 1994)

Df(2L)Adh-nBR127

(Fossett et al., 1994)

Df(2L)Adh-nBR128

(Fossett et al., 1994)

Df(2L)Adh-nBR129

(Fossett et al., 1994)

Df(2L)Adh-nBR131

(Roote, 1996.1.11, Roote, 1996.8, Fossett et al., 1994)

Df(2L)b75

(Eriksen et al., 2000, Woodruff and Ashburner, 1979)

Df(2L)b80c1

(Ashburner et al., 1982)

Df(2L)b88f32

(Eriksen et al., 2000)

Df(2L)el20

(Roote, 1995.5.13)

Df(2L)el77

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Df(2L)fn1

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn2

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn3

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn5

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn15

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn27

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn36

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)GT4

(Gubb et al., 1990)

Df(2L)osp29

(Eriksen et al., 2000)

Df(2L)r10

(Eriksen et al., 2000)

Df(2L)Sco^rv4

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Df(2L)Sco^rv10

(Woodruff and Ashburner, 1979)

Df(2L)Sco^rv14

(Woodruff and Ashburner, 1979)

Df(2L)TE35B-1

(Gubb et al., 1985)

Df(2L)TE35B-2

(Gubb et al., 1985)

Df(2L)TE35B-3

(Gubb et al., 1985)

Df(2L)TE35B-4

(Eriksen et al., 2000, Gubb et al., 1985)

Df(2L)TE35B-6

(Gubb et al., 1985)

Df(2L)TE35B-7

(Gubb et al., 1985)

Df(2L)TE35B-9

(Gubb et al., 1985)

Df(2L)TE35B-10

(Gubb et al., 1985)

Df(2L)TE35B-11

(Gubb et al., 1985)

Df(2L)TE35B-12

(Gubb et al., 1985)

Df(2L)TE35B-15

(Gubb et al., 1985)

Df(2L)TE35BC-1

(Gubb et al., 1984)

Df(2L)TE35BC-3

(Gubb et al., 1984)

Df(2L)TE35BC-4

(Gubb et al., 1984)

Df(2L)TE35BC-7

(Gubb et al., 1984)

Df(2L)TE35BC-8

(Gubb et al., 1984)

Df(2L)TE35BC-24

(Eriksen et al., 2000, Gubb et al., 1984)

Df(2L)TE35BC-28

(Gubb et al., 1984)

Df(2L)TE35BC-29

(Gubb et al., 1984)

Df(2L)TE35BC-31

(Gubb et al., 1984)

Df(2L)TE35BC-34

(Gubb et al., 1984)

Df(2L)TE35BC-35

(Gubb et al., 1984)

Df(2L)W

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

In(2L)75c^LC158.1^R

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

In(2L)75c

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

In(2L)osp²²

(Eriksen et al., 2000)

In(2L)TE35B-13

(Gubb et al., 1985)

T(2;3)Sco^rv7

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

T(Y;2)A80^DR15^P

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

T(Y;2)J165^DP58^P

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

T(Y;2)R15^DJ165^P

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Tp(2;2)A446

(Fossett et al., 1994, Ashburner et al., 1982)

Tp(2;3)dp

(Ashburner et al., 1981)

Not duplicated in

Dp(2;Y)b88b15

(Roote, 1996.8)

Partially disrupted in

Df(2L)noc11

(Roote, 1996.8)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PI

rk^NIG.8930R

lethal, with Scer\GAL4^Act5C.PU

lethal, with Scer\GAL4^how-24B

rk^NIG.8930R

lethal, with Scer\GAL4^T76

rk^GD14383

lethal | larval stage, with Scer\GAL4^Act5C.PI

rk^GD14383

lethal | P-stage, with Scer\GAL4^Act5C.PI

rk^GD14383

lethal | recessive | heat sensitive

rk²

lethal - all die before end of embryonic stage | recessive

rk^11P

lethal - all die before end of P-stage, with Scer\GAL4^69B

rk^GD14383

lethal - all die before end of P-stage, with Scer\GAL4^T80

rk^GD14383

partially lethal

rk^cyl

partially lethal | male

rk⁴

partially lethal - majority die, with Scer\GAL4^Act88F.1.3

rk^NIG.8930R

partially lethal - majority die, with Scer\GAL4^ap-md544

rk^NIG.8930R

partially lethal - majority die, with Scer\GAL4^Mef2.PR

rk^NIG.8930R

partially lethal - majority die (with Df(2L)BSC252)

rk¹

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^MJ33a

rk^GD14383

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

viable (with rk^A376)

rk^b-L

viable (with rk^b-L)

rk^A376

Sterility

Allele

fertile

fertile (with rk^A376)

rk^b-L

fertile (with rk^b-L)

rk^A376

Other Phenotypes

Allele

behavior defective

rk⁴

body color defective

rk^crm

body color defective, with Scer\GAL4^pnr-MD237

rk^GD14383

cell migration defective | adult stage | female, with Scer\GAL4^slbo.2.6

rk^GD14383

cell migration defective | adult stage | female | somatic clone

decreased cell death

rk¹

decreased cell death | adult stage

rk⁴

developmental rate defective

rk⁴

developmental rate defective | larval stage | conditional, with Scer\GAL4^αTub84B.PL

rk^JF02678

increased cell number

rk¹

increased cell number, with Scer\GAL4^how-24B

increased occurrence of cell division | adult stage

rk¹

increased occurrence of cell division | adult stage, with Scer\GAL4^how-24B

increased occurrence of cell division | adult stage, with Scer\GAL4^Mef2.PU

increased occurrence of cell division | adult stage (with rk¹)

rk⁴

increased occurrence of cell division | adult stage (with rk⁴)

rk¹

locomotor behavior defective | adult stage, with Scer\GAL4^C855a

rk^GD14383

mating defective

rk⁴

mating defective | male

rk⁴

small body

rk³

some die during embryonic stage | recessive

rk^11P

some die during pharate adult stage, with Scer\GAL4^69B

rk^GD14383

some die during pharate adult stage, with Scer\GAL4^T80

rk^GD14383

visible

visible, with Scer\GAL4^Act5C.PI

rk^GD15

visible, with Scer\GAL4^Act88F.1.3

rk^NIG.8930R

visible, with Scer\GAL4^ap-md544

rk^NIG.8930R

visible, with Scer\GAL4^C855a

rk^GD14383

visible, with Scer\GAL4^GMR.PU

rk^GD14383

visible, with Scer\GAL4^Mef2.PR

rk^NIG.8930R

visible (with Df(2L)BSC252)

rk¹

visible | recessive

visible | recessive | heat sensitive

rk²

Phenotype manifest in

Allele

abdomen

rk^cyl

adult cuticle

rk⁴

adult cuticle, with Scer\GAL4^69B

rk^GD14383

adult cuticle, with Scer\GAL4^Act5C.PI

rk^GD14383

adult midgut

rk¹

adult midgut, with Scer\GAL4^how-24B

border follicle cell, with Scer\GAL4^slbo.2.6

rk^GD14383

border follicle cell | somatic clone

eye, with Scer\GAL4^GMR.PU

rk^GD14383

haltere

rk^cyl

intestinal stem cell

rk¹

intestinal stem cell, with Scer\GAL4^how-24B

leg

leg, with Scer\GAL4^69B

rk^GD14383

macrochaeta

rk³

mesothoracic tergum, with Scer\GAL4^pnr-MD237

rk^GD14383

metathoracic leg

rk^unspecified

metathoracic tarsal segment, with Scer\GAL4^C855a

rk^GD14383

neurosecretory neuron

rk⁴

posterior scutellar bristle

rk^crm

pupal cuticle, with Scer\GAL4^Act5C.PI

rk^GD14383

tarsal segment

tarsal segment (with Df(2L)BSC252)

rk¹

tarsal segment | heat sensitive

rk²

wing

wing, with Scer\GAL4^69B

rk^GD14383

wing, with Scer\GAL4^Act5C.PI

wing, with Scer\GAL4^Act88F.1.3

rk^NIG.8930R

wing, with Scer\GAL4^ap-md544

rk^NIG.8930R

wing, with Scer\GAL4^C855a

rk^GD14383

wing, with Scer\GAL4^Mef2.PR

rk^NIG.8930R

wing (with Df(2L)BSC252)

wing | heat sensitive

rk²

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (12)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\LGR5

8 of 15

Yes

Hsap\LGR4

6 of 15

Yes

Hsap\LGR6

5 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (12)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Lgr5

8 of 15

Yes

Mmus\Lgr4

5 of 15

Yes

Mmus\Lgr6

5 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (12)

Rnor\Lgr5

7 of 13

Yes

Rnor\Lgr4

5 of 13

Yes

Rnor\Lgr6

5 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (10)

Xtro\lgr5

2 of 12

Yes

Xtro\drc3

1 of 12

Xtro\fshr

1 of 12

Xtro\LOC101734523

1 of 12

Yes

Xtro\lrrc4b

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (21)

Drer\lgr4

3 of 15

Yes

Drer\drc3

1 of 15

Drer\fshr

1 of 15

Drer\lgr6

1 of 15

Yes

Drer\lhcgr

1 of 15

Drer\LOC103911657

1 of 15

Yes

Drer\lrfn4b

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\tlr8b

1 of 15

Drer\tshr

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\fshr-1

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Cele\lron-14

1 of 15

Arabidopsis thaliana (thale-cress) (6)

Atha\AT1G06840

1 of 9

Yes

Atha\AT2G25790

1 of 9

Yes

Atha\GHR1

1 of 9

Yes

Atha\RLP12

1 of 9

Yes

Atha\RLP35

1 of 9

Yes

Atha\RLP38

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919011S )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\rk

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21416

Drosophila persimilis

Dper\GL21062

Drosophila willistoni

Dwil\GK15106

Drosophila virilis

Dvir\GJ18069

Drosophila mojavensis

Dmoj\GI17392

Drosophila grimshawi

Dgri\GH10603

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915014V )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17011063

Glossina morsitans

Tsetse fly

Gmor\GMOY003161

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_00053

Mayetiola destructor

Hessian fly

Mdes\Mdes018870

Mayetiola destructor

Hessian fly

Mdes\Mdes017538

Aedes aegypti

Yellow fever mosquito

Aaeg\GPRGRK

Anopheles darlingi

American malaria mosquito

Adar\ADAC000540

Anopheles gambiae

Malaria mosquito

Agam\GPRRK

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ011619

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03P8 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA009306

Danaus plexippus

Monarch butterfly

Dple\DPOGS205818-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL009969

Apis florea

Little honeybee

Aflo\2318555172

Apis mellifera

Western honey bee

Amel\GB45694

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP19125

Bombus terrestris

Buff-tailed bumblebee

Bter\15213125

Linepithema humile

Argentine ant

Lhum\LH12571

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0016f2

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG012149

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_00009

Tribolium castaneum

Red flour beetle

Tcas\TC008163

Pediculus humanus

Human body louse

Phum\PHUM061060

Rhodnius prolixus

Kissing bug

Rpro\RPRC001663

Cimex lectularius

Bed bug

Clec\CLEC004921

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000221

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_04264

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03MA )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR006061

Ixodes scapularis

Black-legged tick

Isca\ISCW015788

Stegodyphus mimosarum

African social velvet spider

Smim\KFM64533.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur11g03260

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_45411

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G041V )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-GhrL

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ghr

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000018199

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (22)

Gene Symbol

Score

Source

Alignment

3 of 10

3 of 10

3 of 10

ltl

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Con

1 of 10

kek1

1 of 10

kek3

1 of 10

Lapsyn

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

LGR5; leucine rich repeat containing G protein-coupled receptor 5

8 of 15

606667

LGR4; leucine rich repeat containing G protein-coupled receptor 4

6 of 15

606666

BONE MINERAL DENSITY QUANTITATIVE TRAIT LOCUS 17; BMND17

LGR6; leucine rich repeat containing G protein-coupled receptor 6

5 of 15

606653

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Scopelliti et al., 2014)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0003255

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-50

Cytogenetic map

34E5-34E6

Sequence location

2L:13,982,743..14,001,248 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

34E5-34E6

Limits computationally determined from genome sequence between P{lacW}Sos^k05224&P{lacW}RpII33^k05605 and P{lacW}l(2)34Fa^k00811&P{PZ}wb⁰⁹⁴³⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

34E-34E

(Eriksen, 1999.4.12)

Experimentally Determined Recombination Data

Location

2-50

(FlyBase, 2016)

2-45.4

(Comeron et al., 2012)

2-48.2

(Meyer, 1963, Meyer et al., 1950)

2-48.8

Left of (cM)

(Woodruff and Ashburner, 1979)

Right of (cM)

(Woodruff and Ashburner, 1979)

NimA

Notes

Stocks and Reagents

Stocks (22)

Bloomington

3590

rk⁴

3589

rk¹ cn¹ bw¹

e02003

e02668

f08085

107114

rk⁴

107113

rk¹ cn¹ bw¹

VDRC

v105360

P{KK108009}VIE-260B

v904

w¹¹¹⁸; P{GD15}v904

v905

w¹¹¹⁸; P{GD15}v905

v29931

w¹¹¹⁸; P{GD14383}v29931

v29932

w¹¹¹⁸; P{GD14383}v29932

More stocks available...

Genomic Clones (25)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (8)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003255

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003255

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34819

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: rk CG8930

(Guan, 1999.11)

Source for identity of: glycoprotein-hormone-receptor-II CG8930

(FlyBase, 1996-)

Source for database merge of

Additional comments

Source for identity of glycoprotein-hormone-receptor-II CG8930 was sequence comparison ( date:000504 ).

(FlyBase, 1996-)

Other Comments

The rk receptor and its signal Burs appear to be involved in intercellular communication within the border follicle cell cluster itself during border cell migration, as the Burs signal is only required in the border cells.

(Anllo and Schüpbach, 2016)

rk is necessary for the death and resorption of wing blade cells after eclosion.

(Kimura et al., 2004)

Strong alleles have legs, especially metathoracic ones, flattened and bent. Femora and tibiae bowed in middle; first two tarsal joints shortened, bent and flattened; last three tarsal joints almost fused. Wings unexpanded, resembling those of pu. Posterior scutellar bristles erect and crossed. Viability may be reduced. Weak alleles do not show the leg phenotype, or they overlap wild type; may have partially expanded wings and normal posterior scutellar bristles. Even strong alleles more extreme when hemizygous with rk deficiencies.

(Lindsley and Zimm, 1992)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 40 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34819

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003255

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003255

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

rk_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8930

Flygut - An atlas of the Drosophila adult midgut

FBgn0003255

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34819

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008163

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8930

modMine - A data warehouse for the modENCODE project

FBgn0003255

Linkouts

ApoDroso - Functional genomic database for photoreceptor development, survival and function

10919-rk[11P]

DroID - A comprehensive database of gene and protein interactions.

FBgn0003255

DRSC - Results frm RNAi screens

FBgn0003255

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0003255

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8930-RA

FlyMine - An integrated database for Drosophila genomics

FBgn0003255

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

BG:DS00180.13

(Ashburner et al., 1999, Ashburner et al., 1999.10.12)

CG8930

(Yu et al., 2016, Nässel and Winther, 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Bhutkar et al., 2008, Hauser et al., 2006)

CT25644

(Hynes and Zhao, 2000)

DLGR-2

(Vanden Broeck, 2001, Eriksen et al., 2000)

DLGR2

(Liu and Jin, 2017, An et al., 2012, Hauser et al., 2006, Hauser et al., 2006, Luo et al., 2005, Mendive et al., 2005, Sudo et al., 2005)

DLgr2

(Liu and Jin, 2017)

LGR2

(Nishi et al., 2002, Nishi, 2000.6.2)

Lgr2

(Jaszczak et al., 2016)

(Bonfini et al., 2016)

dlgr2

(Scopelliti et al., 2019)

dlgr2/rk

(Scopelliti et al., 2019)

lgr2

(Vallejo et al., 2015)

rickets

(Anllo and Schüpbach, 2016)

rk/CG8930

(Hewes and Taghert, 2001.7.31)

Name Synonyms

Glycoprotein hormone receptor II

(Metpally and Sowdhamini, 2005)

Rickets

(Yu et al., 2016, Caers et al., 2012)

glycoprotein hormone receptor II

(Eriksen, 1999.4.12)

glycoprotein-hormone-receptor-II

leucine-rich repeat-containing G protein-coupled receptor 2

(Nishi, 2000.6.2)

rickets

(Jaszczak et al., 2016, Kim et al., 2015, Harwood et al., 2014, White and Ewer, 2014, An et al., 2012, Diao and White, 2012, Loveall and Deitcher, 2010, Natzle et al., 2008, Peabody et al., 2008, Davis et al., 2007)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (128)

Report Sections

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