protein is expressed in the differentiated neurons of the middle domain of the medulla anlage from third instar larvae and in pupa until 12h APF. Its domain is located in between the concentric domains of vvl
distally and hth
proximally. Thereafter and in adults, run
protein is found in layers 8 to 10 of the medulla.
Expression assayed at stages 9, 11, 13, and 17. Expression may be continuous between assayed stages in some tissues.
Expression in procephalic neuroblasts stage 9-11: deuterocerebrum - d1-3, d5, v2, v5-7; protocerebrum - ad3, ad11, cd8-12, cd15-20, pd3, pd9, pd12, pd13, pd17, pd18, pv1
protein is localized to row 2/3 in wild type embryos but expands posteriorly into row 4 in wg-
is expressed extensively in the developing nervous system. Neural expression is first observed in delaminating neuroblasts at 4.5 hours of development (about 5 per hemisegment). Expression is also observed in daughter ganglion mother cells and becomes increasingly complex as development proceeds. Double staining with antibodies against eve
protein show that one site of run
protein expression is in all of the neurons and GMCs of the EL neuron lineage. Another site of run
protein expression is in some of the U neurons (CQ neurons).
protein is expressed in a pair rule pattern of 7 stripes in the blastoderm embryo. The position of run
protein stripes was compared to that of other segmentation genes. The h
protein stripes are anterior to and complementary to the run
protein stripes. The eve
protein stripes lie anterior to the run
protein stripes but overlap them partially. Two rows of eve
expression are anterior to a two-row region of overlap with run
followed by two rows of run
expression and then two rows of non-expression before the next eve
stripe. Similarly, run
double staining shows that the run
stripes are anterior to the ftz
stripes but are partially overlapping. In early germ band extension the run
pattern changes from a pair rule pattern to a segmental pattern and in addition, expression is observed in the head. Two groups of cells are stained in the head, a dorso-lateral group of 10 cells and a ventral group of 7 less intensely staining cells. The pattern becomes more complex at full germ band extension. At this stage in the segmented part of the embryo, the staining is strongest just off the midline and fades laterally. The stained cells along the midline probably correspond to neuroblasts. During germ band retraction, staining becomes evident in both the CNS and the PNS. Roughly 50 cells per hemi-segment are stained in the CNS at the completion of germ band retraction. PNS staining is also evident.