sbd, Sb-sbd, stubbloid, Stubble-stubbloid
Gene model reviewed during 5.53
Low-frequency RNA-Seq exon junction(s) not annotated.
May activate itself by proteolytic cleavage.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sb using the Feature Mapper tool.
Sb is expressed in several discrete areas in the wing pouch, mostly in the anterior compartment.
Sb transcripts are detected in 12-18hr embryos, in early prepupae, and in 36hr pupae on northern blots. In cultured discs, they are evident after 1-3hrs incubation with ecdysone. By in situ hybridization, Sb are detected in prepupal imaginal discs, primarily in regions that undergo profound ecdysone-dependent shape changes to form appendages such as legs, wings, halteres, and antennae. In 29hr pupae, transcripts are present in leg and wing epidermis.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Sb in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Genetic combinations with mutants of nub cause additive phenotypes.
Definitive denticle belt phenotypes (abnormal hairs and/or bristles) are foreshadowed by abnormal organisation of the actin cytoskeleton in embryonic epidermal cells.
There are no viable alleles than can safely be called nulls.
Sb transcript is ecdysone-inducible in imaginal discs cultured in vitro: structure of encoded transmembrane protein suggests it could be self activating by proteolytic cleavage.
Sb product may have dual function; to detach imaginal disc cells from extracellular matrix via its extracellular proteolytic domain and to transmit signal to intracellular domain to modify cytoskeleton and facilitate cell changes.
Many homoallelic and heteroallelic combinations, involving Sb alleles, display, in addition to shortened thickened bristles, what is termed the malformed syndrome. It consists of wings greatly reduced in size and short, thick, and twisted mesothoracic and metathoracic legs. Sb genotypes defective in elongation of appendage imaginal discs. This effect is exaggerated in a temperature-sensitive fashion in many genotypes that contain mutants in br; for example, females that are heterozygous both for Sb1 or Sb63b and any of a number of br alleles or a br deficiency display a strong enhancement of the short-wing deformed-leg syndrome. Similarly the malformed syndrome is engendered in br/Y males by the presence of Sb/+.
Mutant alleles are useful as markers in clonal analysis.
Mutant individuals display short and thick bristles.
Bridges, 21st April 1923.