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Gene Dmel\Sb

General Information
Symbol	Dmel\Sb	Species	D. melanogaster
Name	Stubble	Annotation symbol	CG4316
Feature type	protein_coding_gene	FlyBase ID	FBgn0003319
Gene Model Status	Current	Stock availability	9836 publicly available
Also Known As	sbd, Sb-sbd
Genomic Location
Chromosome (arm)	3R	Recombination map	3-58.22
Cytogenetic map	89B4-89B6	Sequence location	3R:11,954,451..11,971,006 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Stubble is referred to in FlyBase by the symbol Dmel\Sb (CG4316, FBgn0003319). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: serine-type endopeptidase activity. There is experimental evidence that it is involved in the biological process: actin filament bundle assembly; cytoskeleton organization. 61 alleles are reported. The phenotypes of these alleles are annotated with: leg; ocellar bristle; macrochaeta; chaeta; ventral thoracic disc; tibia; denticle belt; metatarsus. It has one annotated transcript and one annotated polypeptide. Protein features are: Peptidase S1; Peptidase S1, trypsin family, active site; Peptidase S1A, chymotrypsin-type; Trypsin-like cysteine/serine peptidase domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed during late pupal stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1638512_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Sb. Gene sequence location is 3R:11954451..11971006.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Sb: Stubble Sb: Stubble Edith M. Wallace, unpublished. Bristles of Sb/+ less than one-half normal length and somewhat thicker than wild type. Fiber bundles in bristle shafts smaller and more numerous than in wild type; occupy a third as much of the bristle cross-sectional area in Sb as in wild type (Overton, 1967, J. Morph. 122: 367-80). Developmental studies by Lees and Waddington [1943, Proc. Roy. Soc. (London), Ser. B. 131: 87-110 (fig.)] show that trichogen is shifted to lie more or less on the level of the tormogen. Most alleles viable as homozygotes; the only exceptions are Sb1 and its derivative SbV; they may be defective for an adjacent locus. Many homoallelic and heteroallelic combinations, involving both Sb and sbd alleles, display, in addition to shortened thickened bristles, what is termed by Beaton et al., the malformed syndrome (Moore, 1935, DIS 3: 27); it consists of wings greatly reduced in size and short, thick, and twisted mesothoracic and metathoracic legs. Sb genotypes defective in elongation of appendage imaginal discs (Beaton et al.). This effect is exaggerated in a temperature-sensitive fashion in many genotypes that contain mutants in BRC; for example, females that are heterozygous both for Sb1 or Sb63 and any of a number of br alleles or a br deficiency display a strong enhancement of the short-wing deformed-leg syndrome. Similarly the malformed syndrome is engendered in br/Y males by the presence of Sb/+ (Beaton et al.). Sb1/sbd2 more extreme than Sb1/+. sbd2 Sb1 behaves as a recessive sbd allele but is homozygous lethal; rare escapers more extreme and easy to recognize (Davis, 1971, Mol. Gen. Genet. 113: 251-72). Classifiable in single dose in triploids. RK1. Sb63b Bristles of Sb63/+ somewhat shorter and thicker than Sb. Wings and legs normal. Homozygote shows reduced viability, short, thick bristles, small wings, and short deformed legs (Beaton et al.). Sb63b/Sb viable and fertile, more extreme than either heterozygote. RK1. SbSpi: Stubble-Spike Bristles of SbSpi/+ about two-thirds normal length. Wings and legs normal. Bristles of homozygote one-fourth normal length. Wings reduced, crumpled, or blistered. Legs often short and bowed. SbSpi/Sb viability about 30% wild type. Bristles and wings shorter than homozygous SbSpi. RK1. SbV: Stubble-Variegated SbV/+ has mixture of wild-type and Sb bristles. In X/X/Y female and X/Y/Y male, bristles nearly all Sb. In X/0 male, bristles usually all wild type. SbV/Sb and homozygous SbV are lethal. RK1A. SbW: Stubble-Wisconsin Heterozygotes have short stout bristles; homozygotes die as larvae. Tends to persist in laboratory populations (Friedenberg and Chung, 1967, Genetics 57: 957-67). sbd: stubbloid sbd: stubbloid From Dobzhansky, 1930, Z. Indukt. Abstamm. Vererbungsl. 54: 427-57. Bristles short but usually slightly longer than in Sb/+. One or both wings often shortened and crumpled at base. Tibia and femur often shortened, thickened, and bowed. sbd genotypes interact synergistically with br genotypes to exaggerate reductions in wing length and short gnarled legs (Beaton et al.). Viability somewhat low. RK2. sbd2 Most bristles about three-fourths normal length although some (i.e., posterior postalars) are shorter. Less extreme than sbd. sbd2/Sb has shorter bristles than homozygous sbd2 or Sb/+. sbd2 Sb/+ + has wild-type bristles (Lewis, 1951, Cold Spring Harbor Symp. Quant. Biol. 16: 159-74). RK1. sbdl: stubbloid-lethal sbdl/sbd is sbd; sbdl/Sb is lethal. RK2A.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 89B4-89B6   Limits computationally determined from genome sequence between P{PZ}blp01618 and P{PZ}gish04895&P{EP}EP3171
Experimentally Determined Cytological Location
Cytogenetic map Notes References 89B9-89B10   (determined by in situ hybridisation)   (Appel et al., 1993)   Bin1 is located ~43 kb distal to Sb.   (Jones, 2000.3.9)
Experimentally Determined Recombination Data
Location	3-58.2 (Duncan and Kaufman, 1975, Green et al., 1990, Neel, 1942) 3-58.22
Left of (cM)	ss (Struhl, 1984)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Sb for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Sb-RA FBtr0083250  NM_057361   3778   787
Additional Transcript Data & Comments
Reported size (kB)	3.8 (northern blot) (Appel et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Sb-PA   FBpp0082704   85.1   787   10.09     NP_476709   AAF55277
Additional Polypeptide Data & Comments
Reported size (kDa)	786 (aa) (Appel et al., 1993)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Peptidase S1, trypsin family, active site (IPR018114) Peptidase S1 (IPR001254) Peptidase S1A, chymotrypsin-type (IPR001314) Trypsin-like cysteine/serine peptidase domain (IPR009003) MEROPS - An information resource for peptidases and the proteins that inhibit them • S01.225
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA952175   AE014297 AAF55277   AC007760   AI512658   AW943731   AY119618 AAM50272   BI228428   CO177133   CO269303   CZ468024   CZ468025   EC051969   EC053490   EC055477   EC055908   EC056274   EC056590   EC059667   EC204286   GH747858   GH748395   GH753221   GH760368   GH774892   GH781120   GH781335   GH792628   GH801158   GH811239   GH813305   GH813502   GH818102   GH819306   GH820753   GH824236   GH835631   GH843059   GH857407   GH858837   GH859678   GH860064   GH861667   GH863777   GH869540   GH870454   GH873129   GH873281   GH891313   GH893770   GH895541   GH905086   GH915163   GH918746   GH924624   GH926771   GH932344   GH932815   GH935052   GH937596   L11451 AAA28918
UniProtKB/Swiss-Prot	Q05319
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference pupal stage   epidermis of leg | presumptive   (Appel et al., 1993) epidermis of wing | presumptive   (Appel et al., 1993) imaginal disc   (Appel et al., 1993)
Additional Descriptive Data
	Sb transcripts are detected in 12-18hr embryos, in early prepupae, and in 36hr pupae on northern blots. In cultured discs, they are evident after 1-3hrs incubation with ecdysone. By in situ hybridization, Sb are detected in prepupal imaginal discs, primarily in regions that undergo profound ecdysone-dependent shape changes to form appendages such as legs, wings, halteres, and antennae. In 29hr pupae, transcripts are present in leg and wing epidermis. (Appel et al., 1993)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    3.175 Larval Midgut    4.9 Larval Hindgut    4.4 Larval Malpighian Tubules    3 Larval Fat Body    10.8 Larval Salivary Gland    3.2 Larval Trachea    10.825 Larval Carcass    14.9 Adult Head    7.8 Adult Eye    8.175 Adult Brain    5.6 Adult Thoracic-Abdominal Ganglion    6.1 Adult Crop    11.1 Adult Midgut    4.6 Adult Hindgut    10.6 Adult Malpighian Tubules    7.7 Adult Fat Body    5.9 Adult Salivary Gland    8.4 Adult Heart    5.05 Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca    5.8 Adult Ovary    2.3 Adult Testis    2.5 Adult Male Accessory Gland    6.7 Adult Carcass    10.9 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    3.175 Larval Midgut    4.9 Larval Hindgut    4.4 Larval Malpighian Tubules    3 Larval Fat Body    10.8 Larval Salivary Gland    3.2 Larval Trachea    10.825 Larval Carcass    14.9 Adult Head    7.8 Adult Eye    8.175 Adult Brain    5.6 Adult Thoracic-Abdominal Ganglion    6.1 Adult Crop    11.1 Adult Midgut    4.6 Adult Hindgut    10.6 Adult Malpighian Tubules    7.7 Adult Fat Body    5.9 Adult Salivary Gland    8.4 Adult Heart    5.05 Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca    5.8 Adult Ovary    2.3 Adult Testis    2.5 Adult Male Accessory Gland    6.7 Adult Carcass    10.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    3.175 Larval Midgut    4.9 Larval Hindgut    4.4 Larval Malpighian Tubules    3 Larval Fat Body    10.8 Larval Salivary Gland    3.2 Larval Trachea    10.825 Larval Carcass    14.9 Adult Head    7.8 Adult Eye    8.175 Adult Brain    5.6 Adult Thoracic-Abdominal Ganglion    6.1 Adult Crop    11.1 Adult Midgut    4.6 Adult Hindgut    10.6 Adult Malpighian Tubules    7.7 Adult Fat Body    5.9 Adult Salivary Gland    8.4 Adult Heart    5.05 Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca    5.8 Adult Ovary    2.3 Adult Testis    2.5 Adult Male Accessory Gland    6.7 Adult Carcass    10.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    3.175 Larval Midgut    4.9 Larval Hindgut    4.4 Larval Malpighian Tubules    3 Larval Fat Body    10.8 Larval Salivary Gland    3.2 Larval Trachea    10.825 Larval Carcass    14.9 Adult Head    7.8 Adult Eye    8.175 Adult Brain    5.6 Adult Thoracic-Abdominal Ganglion    6.1 Adult Crop    11.1 Adult Midgut    4.6 Adult Hindgut    10.6 Adult Malpighian Tubules    7.7 Adult Fat Body    5.9 Adult Salivary Gland    8.4 Adult Heart    5.05 Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca    5.8 Adult Ovary    2.3 Adult Testis    2.5 Adult Male Accessory Gland    6.7 Adult Carcass    10.9 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    3.175 Larval Midgut    4.9 Larval Hindgut    4.4 Larval Malpighian Tubules    3 Larval Fat Body    10.8 Larval Salivary Gland    3.2 Larval Trachea    10.825 Larval Carcass    14.9 Adult Head    7.8 Adult Eye    8.175 Adult Brain    5.6 Adult Thoracic-Abdominal Ganglion    6.1 Adult Crop    11.1 Adult Midgut    4.6 Adult Hindgut    10.6 Adult Malpighian Tubules    7.7 Adult Fat Body    5.9 Adult Salivary Gland    8.4 Adult Heart    5.05 Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca    5.8 Adult Ovary    2.3 Adult Testis    2.5 Adult Male Accessory Gland    6.7 Adult Carcass    10.9 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    3.175 Larval Midgut    4.9 Larval Hindgut    4.4 Larval Malpighian Tubules    3 Larval Fat Body    10.8 Larval Salivary Gland    3.2 Larval Trachea    10.825 Larval Carcass    14.9 Adult Head    7.8 Adult Eye    8.175 Adult Brain    5.6 Adult Thoracic-Abdominal Ganglion    6.1 Adult Crop    11.1 Adult Midgut    4.6 Adult Hindgut    10.6 Adult Malpighian Tubules    7.7 Adult Fat Body    5.9 Adult Salivary Gland    8.4 Adult Heart    5.05 Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca    5.8 Adult Ovary    2.3 Adult Testis    2.5 Adult Male Accessory Gland    6.7 Adult Carcass    10.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    3.175 Larval Midgut    4.9 Larval Hindgut    4.4 Larval Malpighian Tubules    3 Larval Fat Body    10.8 Larval Salivary Gland    3.2 Larval Trachea    10.825 Larval Carcass    14.9 Adult Head    7.8 Adult Eye    8.175 Adult Brain    5.6 Adult Thoracic-Abdominal Ganglion    6.1 Adult Crop    11.1 Adult Midgut    4.6 Adult Hindgut    10.6 Adult Malpighian Tubules    7.7 Adult Fat Body    5.9 Adult Salivary Gland    8.4 Adult Heart    5.05 Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca    5.8 Adult Ovary    2.3 Adult Testis    2.5 Adult Male Accessory Gland    6.7 Adult Carcass    10.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    3.175 Larval Midgut    4.9 Larval Hindgut    4.4 Larval Malpighian Tubules    3 Larval Fat Body    10.8 Larval Salivary Gland    3.2 Larval Trachea    10.825 Larval Carcass    14.9 Adult Head    7.8 Adult Eye    8.175 Adult Brain    5.6 Adult Thoracic-Abdominal Ganglion    6.1 Adult Crop    11.1 Adult Midgut    4.6 Adult Hindgut    10.6 Adult Malpighian Tubules    7.7 Adult Fat Body    5.9 Adult Salivary Gland    8.4 Adult Heart    5.05 Adult VirginFemale Spermatheca    8.5 Adult InseminatedFemale Spermatheca    5.8 Adult Ovary    2.3 Adult Testis    2.5 Adult Male Accessory Gland    6.7 Adult Carcass    10.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    7.8   NA  Eye    8.175   NA  Brain    5.6   3.175  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    6.1   NA  Crop    no informative data   4.9  Midgut    4.6   4.4  Hindgut    no informative data   3  Malpighian Tubules    7.7   no informative data  Fat Body    5.9   3.2  Salivary Gland    8.4   NA  Heart    5.05   no informative data  Trachea    NA   NA  VirginFemale Spermatheca    8.5   NA  InseminatedFemale Spermatheca    5.8   NA  Ovary    2.3   NA  Testis    2.5   NA  Male Accessory Gland    6.7   14.9  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    3 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering    10 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    3 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering    10 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    3 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering    10 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    3 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering    10 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    3 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering    10 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    3 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering    10 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    3 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering    10 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    3 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering    10 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed during late pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    5 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    5 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    5 white prepupae new    12 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP    30 pupae 3d postWPP    4 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    5 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    5 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    5 white prepupae new    12 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP  (30) pupae 3d postWPP    4 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    5 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    5 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    5 white prepupae new    12 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP    30 pupae 3d postWPP    4 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    5 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    5 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    5 white prepupae new    12 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP    30 pupae 3d postWPP    4 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    5 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    5 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    5 white prepupae new    12 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP    30 pupae 3d postWPP    4 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    5 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    5 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    5 white prepupae new    12 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP  30 pupae 3d postWPP    4 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    5 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    5 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    5 white prepupae new    12 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP    30 pupae 3d postWPP    4 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    5 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    5 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    5 white prepupae new    12 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP    30 pupae 3d postWPP    4 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_07
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003319 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4316-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal (with Sb63b) Sb70 lethal (with Sb70) Sb63b lethal | embryonic stage | recessive SbEbr20 SbEbr48 SbEbr228 SbEbr448 SbEbr536 SbEbr623 Sbsbd-32 Sbsbd-35 Sbsbd-43 lethal | larval stage Sbsbd-12 Sbsbd-13 Sbsbd-17 Sbsbd-18 Sbsbd-21 Sbsbd-32 Sbsbd-35 Sbsbd-43 Sbsbd-47 Sbsbd-l lethal | larval stage | recessive SbEbr20 SbEbr48 SbEbr228 SbEbr448 SbEbr536 SbEbr623 Sbsbd-12 Sbsbd-13 Sbsbd-17 Sbsbd-18 Sbsbd-21 SbW lethal | pupal stage, with Scer\GAL4pnr-MD237 SbGD473 lethal | recessive Sb1 Sb41k7 Sb63b Sb70 Sbsbd-1 Sbsbd-47 Sbunspecified partially lethal - majority die Sb1 viable SbSpi viable (with Df(3R)sbd105) SbEbr20 SbEbr536 SbEbr623 SbSpi viable | poor (with Sb1) Sb63b viable | poor (with Sb63b) Sb1 viable | reduced SbEbr228 Sbsbd-201 Other Phenotypes Allele visible Sb1 Sbsbd-2 Sbsbd-201 SbSpi visible (with Df(3R)sbd105) SbEbr20 SbEbr536 SbEbr623 visible (with Sbsbd-1) Sbsbd-201 visible (with Sbsbd-201) Sbsbd-1 visible | dominant Sb1 Sb63b Sb70 SbSpi visible | heat sensitive Sbhs.PB visible | heat sensitive, with Df(3R)sbd105 SbCD-TM.hs SbCD-TM-knot.hs SbS737A.hs SbΔprotease.hs visible | recessive SbEbr228 Sbsbd-1 Sbsbd-201 Sbsbd-VX1 Phenotype manifest in Allele adult thorax & chaeta | conditional ts Sbhs.PB adult thorax | dorsal | heat sensitive Sbhs.PB chaeta Sb1 Sb63b SbEbr228 SbSpi Sbsbd-201 chaeta (with Df(3R)sbd105) SbEbr20 SbEbr536 SbEbr623 SbSpi chaeta | heat sensitive, with Df(3R)sbd105 SbS737A.hs SbΔprotease.hs SbCD-TM-knot.hs SbCD-TM.hs denticle belt Sb1 Sbsbd-2 embryonic/first instar larval cuticle | anterior SbEbr48 SbEbr623 leg Sb63b Sb70 SbSpi Sbsbd-201 leg (with Df(3R)sbd105) SbSpi leg (with Sbsbd-1) Sbsbd-201 leg (with Sbsbd-201) Sbsbd-1 leg | heat sensitive Sbhs.PB macrochaeta Sb1 SbW macrochaeta, with Scer\GAL4Act5C.PU SbGD473 metatarsus Sb1 Sb63b microchaeta & actin filament Sbsbd-VX1 microchaeta & actin filament (with Df(3R)sbd45) Sbsbd-VX1 ocellar bristle Sb1 prothoracic femur | heat sensitive Sbhs.PB tibia Sb1 Sb63b ventral thoracic disc Sb1 Sb63b
Classical Alleles ( 50 )
For All Classical Alleles Show
Allele of Sb Class Mutagen Stocks Known lesion Sb1 spontaneous 9680 Yes Sbsbd-2 hypomorphic allele - genetic evidence spontaneous 66 Yes Sbsbd-l X ray 31 -- Sbsbd-1 hypomorphic allele - genetic evidence spontaneous 21 -- Sb63b neomorphic allele - genetic evidence spontaneous 11 Yes SbSpi neomorphic allele - genetic evidence, antimorphic allele - genetic evidence X ray 3 Yes Sbc02563 piggyBac transposase 2 -- SbMB03009 1 -- SbMI04063 1 -- Sbsbd-104 X ray 1 Yes Sb41k7 spontaneous 0 -- Sb70 spontaneous 0 Yes SbEbr20 ethyl methanesulfonate 0 -- SbEbr228 ethyl methanesulfonate 0 -- SbEbr448 ethyl methanesulfonate 0 -- SbEbr48 ethyl methanesulfonate 0 -- SbEbr536 ethyl methanesulfonate 0 -- SbEbr623 ethyl methanesulfonate 0 -- SbR+SE1 ethyl methanesulfonate 0 -- SbR+SE2 ethyl methanesulfonate 0 -- SbR+SX3 X ray 0 -- SbR+SX4 X ray 0 -- Sbsbd-106 X ray 0 Yes Sbsbd-12 X ray 0 -- Sbsbd-13 X ray 0 -- Sbsbd-17 X ray 0 -- Sbsbd-18 X ray 0 -- Sbsbd-201 antimorphic allele - genetic evidence, hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- Sbsbd-202r diepoxybutane 0 Yes Sbsbd-203 Bevatron irradiation 0 -- Sbsbd-204 Bevatron irradiation 0 -- Sbsbd-205r Delta2-3 0 Yes Sbsbd-206 ethyl methanesulfonate 0 -- Sbsbd-207 gamma ray 0 -- Sbsbd-208 spontaneous 0 -- Sbsbd-209r spontaneous 0 -- Sbsbd-210 natural population 0 -- Sbsbd-21 X ray 0 -- Sbsbd-32 X ray 0 -- Sbsbd-35 X ray 0 -- Sbsbd-43 X ray 0 -- Sbsbd-47 X ray 0 -- Sbsbd-GT11 0 Yes Sbsbd-PNR11 ethyl methanesulfonate 0 -- Sbsbd-VE1 ethyl methanesulfonate 0 -- Sbsbd-VE2 0 -- Sbsbd-VE3 ethyl methanesulfonate 0 -- Sbsbd-VX1 loss of function allele X ray 0 Yes Sbunspecified 0 -- SbW 0 --
Alleles Carried on Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Sb Class Mutagen Stocks Known lesion SbGD473 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SbGL01193 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SbHMS02483 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SbKK108003 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SbCD-TM-knot.hs in vitro construct - regulatory fusion in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes SbCD-TM.hs in vitro construct - regulatory fusion in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes SbCD.hs in vitro construct - regulatory fusion in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes Sbhs.PB in vitro construct - regulatory fusion 0 Yes SbNIG.4316R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes SbS737A.hs in vitro construct - regulatory fusion in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes SbΔprotease.hs in vitro construct - regulatory fusion in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel7328 (Ryder, 2004.4.26) Df(3R)sbd26 (Spillmann and Nothiger, 1978, Nelson and Szauter, 1992, Heitzler et al., 1996) Df(3R)sbd45 (Ward et al., 2003, Spillmann and Nothiger, 1978, Nelson and Szauter, 1992, Heitzler et al., 1996) Df(3R)sbd104 (Lewis, 1980, Struhl, 1984) Df(3R)sbd105 (Spillmann and Nothiger, 1978, Nelson and Szauter, 1992, Heitzler et al., 1996) In(3R)sbd12 (Spillmann and Nothiger, 1978) In(3R)sbd17 (Spillmann and Nothiger, 1978) In(3R)sbd21 (Spillmann and Nothiger, 1978) In(3R)sbdl (Heitzler et al., 1996) T(2;3)GT11 (Heitzler et al., 1996) T(2;3)SbV (Heitzler et al., 1996) T(2;3)sbd47 (Spillmann and Nothiger, 1978) T(2;3)sbd106 (Heitzler et al., 1996) Tp(3;3)sbd104 (Heitzler et al., 1996)
Duplicated in	Dp(3;1)O5   Dp(3;1)ry35   Dp(3;3)BK43 (Heitzler et al., 1996) Dp(3;3)BK44 (Heitzler et al., 1996)
Not duplicated in	Dp(3;2)ry+ (Green et al., 1990) Dp(3;3)122.3   Dp(3;3)124.3   Dp(3;3)124.5   Dp(3;3)148.4   Dp(3;3)148.8   Dp(3;3)149.4   Dp(3;3)149.9   Dp(3;3)149.12   Dp(3;3)149.14   Dp(3;3)E(var)88D11 (Green et al., 1990) Ts(1Rt;3Rt)OR60+Ts(1Lt;3Lt)89Bq2 (Heitzler et al., 1996)
Not disrupted in	Df(3R)c(3)G-2 (Nelson and Szauter, 1992) Df(3R)E8-89B-Rev2 (Heitzler et al., 1996) Df(3R)kar5l (Green et al., 1990) Df(3R)pnrD4R+3 (Heitzler et al., 1996) Df(3R)Po2 (Nelson and Szauter, 1992) Df(3R)Po3 (Nelson and Szauter, 1992) Df(3R)Po4 (Nelson and Szauter, 1992) Df(3R)red3l (Green et al., 1990)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data heat-shock construct P{hs-Sb.B} NA P{hs-Sb.CD} NA P{hs-Sb.CD-TM} NA P{hs-Sb.CD-TM-knot} NA P{hs-Sb.S737A} NA P{hs-Sb.Δprotease} NA UAS construct P{GD473} NA P{KK108003} NA P{NIG.4316R} NA P{TRiP.GL01193} NA P{TRiP.HMS02483} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions ?{}Sb1 NA ?{}Sb63b NA blood{}Sb63b NA blood{}Sb70 NA Mi{MIC}SbMI04063 NA PBac{PB}Sbc02563 NA insertion of enhancer trap binary system Mi{ET1}SbMB03009 No
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
actin filament bundle assembly inferred from mutant phenotype (Tilney et al., 2003) cytoskeleton organization inferred from mutant phenotype (Appel et al., 1993)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
serine-type endopeptidase activity inferred from electronic annotation with InterPro:IPR001254, InterPro:IPR001314, InterPro:IPR018114 (FlyBase Curators et al., 2004-) non-traceable author statement (Swiss-Prot Project Members, 1994.6.1, Ross et al., 2003)
Biological Process
CV term Evidence References
cytoskeleton organization non-traceable author statement (Swiss-Prot Project Members, 1994.6.1) morphogenesis of larval imaginal disc epithelium non-traceable author statement (Swiss-Prot Project Members, 1994.6.1) proteolysis inferred from electronic annotation with InterPro:IPR001254, InterPro:IPR001314, InterPro:IPR018114 (FlyBase Curators et al., 2004-) non-traceable author statement (Ross et al., 2003)
Cellular Component
CV term Evidence References
membrane non-traceable author statement (Swiss-Prot Project Members, 1994.6.1) plasma membrane inferred from sequence or structural similarity (Appel et al., 1993)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Sb allele Gene References Sb1 Cu-3 (Meyer, 1952) Rho1 (Bayer et al., 2003) Sb63b E(zip)12-5 (Bayer et al., 2003) E(zip)18-5 (Bayer et al., 2003) E(zip)31-6 (Bayer et al., 2003) LIMK1 (Chen et al., 2004) Rho1 (Bayer et al., 2003, Chen et al., 2004) RhoGEF2 (Bayer et al., 2003) rok (Bayer et al., 2003, Chen et al., 2004) zip (Bayer et al., 2003, Chen et al., 2004) Sb70 E(zip)12-5 (Bayer et al., 2003) E(zip)18-5 (Bayer et al., 2003) E(zip)31-6 (Bayer et al., 2003) LIMK1 (Chen et al., 2004) Rho1 (Bayer et al., 2003, Chen et al., 2004) RhoGEF2 (Bayer et al., 2003) rok (Bayer et al., 2003, Chen et al., 2004) zip (Bayer et al., 2003, Chen et al., 2004) SbEbr20 br (Ward et al., 2003) zip (Ward et al., 2003) SbEbr48 br (Ward et al., 2003) zip (Ward et al., 2003) SbEbr228 br (Ward et al., 2003) SbEbr448 br (Ward et al., 2003) zip (Ward et al., 2003) SbEbr536 zip (Ward et al., 2003) SbEbr623 Rho1 (Ward et al., 2003) zip (Ward et al., 2003) Sbsbd-1 E(zip)12-5 (Bayer et al., 2003) E(zip)18-5 (Bayer et al., 2003) E(zip)31-6 (Bayer et al., 2003) RhoGEF2 (Bayer et al., 2003) rok (Bayer et al., 2003) zip (Bayer et al., 2003) Sbsbd-201 E(zip)12-5 (Bayer et al., 2003) E(zip)18-5 (Bayer et al., 2003) E(zip)31-6 (Bayer et al., 2003) RhoGEF2 (Bayer et al., 2003) rok (Bayer et al., 2003) zip (Bayer et al., 2003) SbSpi Rho1 (Bayer et al., 2003) Sbunspecified E(f) (Belgovsky, 1937) unspecified br (Hammonds and Fristrom, 2006, Gotwals and Fristrom, 1991, Ward et al., 2003) l(2)60Dc (von Kalm et al., 1995) Rho1 (Bayer et al., 2003) zip (Hammonds and Fristrom, 2006)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0003319
Orthologs
OrthoDB Orthologs (156) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6256CH Dipteran inclusive ortholog search EOG612MF4 Insect inclusive ortholog search EOG6KWHP5 Arthropod inclusive ortholog search EOG6HX3GG Metazoa inclusive ortholog search EOG6NKQP1
Orthologs in Drosophila Species (EOG6256CH)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Sb     Drosophila simulans   Dsim\GD19075 Y   Drosophila sechellia   Dsec\GM24286 Y   Drosophila erecta   Dere\GG16980 Y   Drosophila yakuba   Dyak\GE24370 Y   Drosophila ananassae   Dana\GF16156 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18102 Y   Drosophila persimilis   Dper\GL12221 Y   Drosophila willistoni   Dwil\GK13263 Y   Drosophila virilis   Dvir\GJ23384 Y   Drosophila mojavensis   Dmoj\GI22656 Y   Drosophila grimshawi   Dgri\GH18766 Y
Orthologs in non-Drosophila Dipterans (EOG612MF4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL005906   Anopheles gambiae Malaria mosquito  Agam\CLIPD8   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ008568
Orthologs in non-Dipteran Insects (EOG6KWHP5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\SP16   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG001466   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH20043   Atta cephalotes Leafcutter ant  Acep\ACEP27703   Camponotus floridanus Florida carpenter ant  Cflo\CFLO19812   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21359   Linepithema humile Argentine ant  Lhum\LH21126   Pogonomyrmex barbatus Red harvester ant  Pbar\PB25910   Solenopsis invicta Red fire ant  Sinv\SINV12876   Acyrthosiphon pisum Pea aphid  Apim\ACYPI004545   Bombyx mori Silkmoth  Bmor\BGIBMGA011594   Pediculus humanus Human body louse  Phum\PHUM547720   Tribolium castaneum Red flour beetle  Tcas\P36
Orthologs in non-Insect Arthropods (EOG6HX3GG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_306713
Orthologs in non-Arthropod Metazoa (EOG6NKQP1)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\try-4 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016868gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_010750gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_025575gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020315gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_005427gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_010205gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_026191gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000377gn Y Danio rerio Zebrafish  Drer\ENSDARG00000043173 Y Danio rerio Zebrafish  Drer\ela2 Y Danio rerio Zebrafish  Drer\zgc:112285 Y Danio rerio Zebrafish  Drer\ENSDARG00000010378 Y Danio rerio Zebrafish  Drer\f9b Y Danio rerio Zebrafish  Drer\zgc:112368 Y Danio rerio Zebrafish  Drer\si:dkey-57c15.4 Y Danio rerio Zebrafish  Drer\ENSDARG00000068726 Y Danio rerio Zebrafish  Drer\masp2 Y Danio rerio Zebrafish  Drer\f7 Y Danio rerio Zebrafish  Drer\LOC562139 Y Danio rerio Zebrafish  Drer\zgc:154142 Y Danio rerio Zebrafish  Drer\ENSDARG00000075719 Y Danio rerio Zebrafish  Drer\CR944667.3 Y Danio rerio Zebrafish  Drer\si:dkey-33i11.3 Y Danio rerio Zebrafish  Drer\ENSDARG00000017314 Y Danio rerio Zebrafish  Drer\DKEY-33I11.3 Y Danio rerio Zebrafish  Drer\ela3l Y Danio rerio Zebrafish  Drer\ENSDARG00000076045 Y Danio rerio Zebrafish  Drer\f10 Y Danio rerio Zebrafish  Drer\zgc:92511 Y Danio rerio Zebrafish  Drer\ENSDARG00000089722 Y Danio rerio Zebrafish  Drer\ctrb1 Y Danio rerio Zebrafish  Drer\ela2l Y Danio rerio Zebrafish  Drer\f9a Y Danio rerio Zebrafish  Drer\ENSDARG00000011144 Y Danio rerio Zebrafish  Drer\zgc:112160 Y Danio rerio Zebrafish  Drer\f7i Y Danio rerio Zebrafish  Drer\ctrl Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000033013 Y Xenopus tropicalis Western clawed frog  Xtro\tmprss12 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000023914 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000025253 Y Xenopus tropicalis Western clawed frog  Xtro\cela3b Y Xenopus tropicalis Western clawed frog  Xtro\c1s Y Xenopus tropicalis Western clawed frog  Xtro\cela3a Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000033599 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000014195 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000033339 Y Xenopus tropicalis Western clawed frog  Xtro\f9 Y Xenopus tropicalis Western clawed frog  Xtro\f10 Y Xenopus tropicalis Western clawed frog  Xtro\gprin3 Y Xenopus tropicalis Western clawed frog  Xtro\ctrb1 Y Xenopus tropicalis Western clawed frog  Xtro\cela2a Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000032160 Y Xenopus tropicalis Western clawed frog  Xtro\masp1 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000025157 Y Xenopus tropicalis Western clawed frog  Xtro\hpn Y Xenopus tropicalis Western clawed frog  Xtro\masp2 Y Xenopus tropicalis Western clawed frog  Xtro\c1r Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000033050 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000014270 Y Xenopus tropicalis Western clawed frog  Xtro\ctrb2 Y Gallus gallus Domestic chicken  Ggal\LOC431235 Y Gallus gallus Domestic chicken  Ggal\C1R Y Gallus gallus Domestic chicken  Ggal\LOC771012 Y Gallus gallus Domestic chicken  Ggal\ENSGALG00000004866 Y Gallus gallus Domestic chicken  Ggal\MASP1 Y Gallus gallus Domestic chicken  Ggal\F9 Y Gallus gallus Domestic chicken  Ggal\CELA2A Y Gallus gallus Domestic chicken  Ggal\CTRL Y Gallus gallus Domestic chicken  Ggal\ENSGALG00000006081 Y Gallus gallus Domestic chicken  Ggal\C1S Y Gallus gallus Domestic chicken  Ggal\CTRC Y Gallus gallus Domestic chicken  Ggal\MASP2 Y Mus musculus House mouse  Mmus\C1rl Y Mus musculus House mouse  Mmus\Cela3b Y Mus musculus House mouse  Mmus\Ctrl Y Mus musculus House mouse  Mmus\F9 Y Mus musculus House mouse  Mmus\Hpn Y Mus musculus House mouse  Mmus\Gm13011 Y Mus musculus House mouse  Mmus\Klk15 Y Mus musculus House mouse  Mmus\Cela1 Y Mus musculus House mouse  Mmus\Klk1b22 Y Mus musculus House mouse  Mmus\F10 Y Mus musculus House mouse  Mmus\Gm5077 Y Mus musculus House mouse  Mmus\C1s Y Mus musculus House mouse  Mmus\Cela2a Y Mus musculus House mouse  Mmus\Ctrb1 Y Mus musculus House mouse  Mmus\Tmprss12 Y Mus musculus House mouse  Mmus\C1ra Y Mus musculus House mouse  Mmus\Masp2 Y Mus musculus House mouse  Mmus\C1rb Y Mus musculus House mouse  Mmus\Masp1 Y Rattus norvegicus Norway rat  Rnor\Ctrl Y Rattus norvegicus Norway rat  Rnor\Ctrb1 Y Rattus norvegicus Norway rat  Rnor\Masp2 Y Rattus norvegicus Norway rat  Rnor\Klk15 Y Rattus norvegicus Norway rat  Rnor\Cela3b Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000003430 Y Rattus norvegicus Norway rat  Rnor\F10 Y Rattus norvegicus Norway rat  Rnor\LOC100362912 Y Rattus norvegicus Norway rat  Rnor\C1r Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000004725 Y Rattus norvegicus Norway rat  Rnor\Cela2a Y Rattus norvegicus Norway rat  Rnor\C1rl Y Rattus norvegicus Norway rat  Rnor\Ctrc Y Rattus norvegicus Norway rat  Rnor\C1s Y Rattus norvegicus Norway rat  Rnor\Masp1 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000021097 Y Homo sapiens Human  Hsap\CELA3B Y Homo sapiens Human  Hsap\TMPRSS12 Y Homo sapiens Human  Hsap\MASP1 Y Homo sapiens Human  Hsap\CELA3A Y Homo sapiens Human  Hsap\ENSG00000168925 Y Homo sapiens Human  Hsap\CELA1 Y Homo sapiens Human  Hsap\ELA2A Y Homo sapiens Human  Hsap\CTRL Y Homo sapiens Human  Hsap\KLK15 Y Homo sapiens Human  Hsap\F10 Y Homo sapiens Human  Hsap\CTRB2 Y Homo sapiens Human  Hsap\ENSG00000159403 Y Homo sapiens Human  Hsap\CELA2B Y Homo sapiens Human  Hsap\CTRC Y Homo sapiens Human  Hsap\FIX F9 Y Homo sapiens Human  Hsap\MASP2 Y Homo sapiens Human  Hsap\C1S Y Homo sapiens Human  Hsap\HPN Y
Human Orthologs (18)
Gene OMIM HGNC Hsap\CELA3B     Hsap\TMPRSS12     Hsap\MASP1     Hsap\CELA3A     Hsap\ENSG00000168925     Hsap\CELA1     Hsap\ELA2A     Hsap\CTRL     Hsap\KLK15     Hsap\F10     Hsap\CTRB2     Hsap\ENSG00000159403     Hsap\CELA2B     Hsap\CTRC     Hsap\FIX F9     Hsap\MASP2     Hsap\C1S     Hsap\HPN
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD19075 Drosophila sechellia Dsec\GM24286 Drosophila erecta Dere\GG16980 Drosophila yakuba Dyak\GE24370 Drosophila ananassae Dana\GF16156 Drosophila pseudoobscura pseudoobscura Dpse\GA18102 Drosophila persimilis Dper\GL12221 Drosophila willistoni Dwil\GK13263 Drosophila virilis Dvir\GJ23384 Drosophila mojavensis Dmoj\GI22656 Drosophila grimshawi Dgri\GH18766
Stocks & Reagents
Stocks Listed in FlyBase ( 9836 )
Bloomington	589 Sbsbd-2 4938 Df(3R)CA/TM3, Sb1 1516 Df(3R)CA3/TM3, Sb1 3066 Dp(3;3)D1/TM3, Sb1 5836 Df(3L)2D/TM6C, Sb1 5837 Df(3L)7C/TM6C, Sb1
Harvard	- PBac{PB}Sbc02563
Kyoto	107316 Df(3R)07280/TM6C, Sb 106360 Df(3R)CA3/TM3, Sb1 106816 Dp(3;3)D1/TM3, Sb1 108456 Df(3L)2D/TM6C, Sb1
VDRC	v108455 P{KK108003}VIE-260B
	More stocks available...
Genomic Clones ( 2 )
	BACR05C20 BACR37I18 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 53 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD44584
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD29259 RE25970
Other clones	166065 CK01.123C.G6 EC051969 EC053490 EC055477 EC055908 EC056274 EC056590 EK052609 EK094059 RP003026949 RP003070114 RP003087557 RP003100336 RP003110291 RP003120513 RP003121025 RP003130677 RP003132850 RP003135159 RP003150195 RP003156901 RP003166583 RP003185288 RP003196704 RP003219067 RP003228439 RP003234669 RP003256597 RP003268302 RP003277137 RP003281550 RP003282739 RP003298781 RP003304368 RP003305678 RP003352520 RP003353808 RP003392187 RP003468531 RP003485532 RP003487893 RP003517135 RP003531531 RP003556875 RP003571993 RP003581813 RP003624020 RP003643008 RP003644145
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003319 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41958
Antibody Information
Other Information
Discoverer
	Bridges, 21st April 1923.
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Genetic combinations with mutants of nub cause additive phenotypes. (Cifuentes and Garcia-Bellido, 1997) Definitive denticle belt phenotypes (abnormal hairs and/or bristles) are foreshadowed by abnormal organisation of the actin cytoskeleton in embryonic epidermal cells. (Dickinson and Thatcher, 1997) There are no viable alleles than can safely be called nulls. (Appel, 1995) Sb transcript is ecdysone-inducible in imaginal discs cultured in vitro: structure of encoded transmembrane protein suggests it could be self activating by proteolytic cleavage. (Appel et al., 1993) Sb product may have dual function; to detach imaginal disc cells from extracellular matrix via its extracellular proteolytic domain and to transmit signal to intracellular domain to modify cytoskeleton and facilitate cell changes. (Appel et al., 1993) The mlf phenotype (malformed syndrome), wing malformations and leg defects, can be produced by a dominant genetic interaction between Sb and br. (Gotwals and Fristrom, 1991) Many homoallelic and heteroallelic combinations, involving Sb alleles, display, in addition to shortened thickened bristles, what is termed the malformed syndrome. It consists of wings greatly reduced in size and short, thick, and twisted mesothoracic and metathoracic legs. Sb genotypes defective in elongation of appendage imaginal discs. This effect is exaggerated in a temperature-sensitive fashion in many genotypes that contain mutants in br; for example, females that are heterozygous both for Sb1 or Sb63b and any of a number of br alleles or a br deficiency display a strong enhancement of the short-wing deformed-leg syndrome. Similarly the malformed syndrome is engendered in br/Y males by the presence of Sb/+. (Beaton et al., 1988) Mutant alleles are useful as markers in clonal analysis. (Lawrence et al., 1986) Mutant individuals display short and thick bristles. (Duncan and Kaufman, 1975) Sb is pseudoallelic to and lies 0.01-0.03 unit to the right of Sbsbd-2. A gain-of-function mutation. Deficiency for the Sb locus produces no dominant phenotype (Lewis, 1951; FBrf0008070).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057361 RefSeq (Proteins) • NP_476709 DDBJ / EMBL / GenBank • AAA28918 AAF55277 AAM50272 AC007760 AI512658 AW943731 AY119618 CZ468024 CZ468025 L11451 Entrez Gene - A searchable database of RefSeq genes. • 41958 UniProtKB/Swiss-Prot • Q05319
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Peptidase S1, trypsin family, active site (IPR018114) Peptidase S1 (IPR001254) Peptidase S1A, chymotrypsin-type (IPR001314) Trypsin-like cysteine/serine peptidase domain (IPR009003) MEROPS - An information resource for peptidases and the proteins that inhibit them • S01.225
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0003319 DRSC - Results from RNAi screens. • FBgn0003319 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003319 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4316-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003319 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41958 modMine - Data generated by the modENCODE project. • FBgn0003319
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	CG4316 (Keleman et al., 2009.8.5, Nam et al., 2012, Ni et al., 2010.12.1) c-SP56 (Ross et al., 2003) Sb (Rendina et al., 2010, Fei et al., 2002, Roegiers et al., 2005, Dietzl et al., 2007, Laffafian et al., 2010, Wang et al., 2009, Sharp and Agrawal, 2008, Di Stefano et al., 2007, Lu et al., 2009, Cook et al., 2010.1.8, Fagegaltier et al., 2009, Neubueser and Hipfner, 2010, Foley et al., 2010, Sasai et al., 2007, Japanese National Institute of Genetics, 2012.5.21, Clark et al., 2012, Gurudatta et al., 2010) sbd (Ward et al., 2003, Lewis, 1998, Staveley et al., 1998, Cifuentes and Garcia-Bellido, 1997, Heitzler et al., 1996, Appel et al., 1993, Green et al., 1990, Kuhn and Woods, 1982, Spillmann and Nothiger, 1978, Ruggiero et al., 2006, Patch et al., 2009) Sb-sbd (von Kalm et al., 1999, Gotwals and Fristrom, 1991, Ruggiero et al., 2006, Fox and Kalm, 2006, Hammonds and Fristrom, 2006) SP56 (Ross et al., 2003) st-sb (Hooper et al., 2001)
Name Synonym	Stubble (Appel, 1993, Andjelkovic et al., 1995, Chanut-Delalande et al., 2006, Benjamin et al., 2008, Morgan and Kalm, 2008, Christophides et al., 2002, Di Stefano et al., 2007, Fagegaltier et al., 2009) Stubble-stubbloid (Hammonds and Fristrom, 2006) stubbloid (Ward et al., 2003, Weisman et al., 2001, Ward et al., 2002, Patch et al., 2009)
Secondary FlyBase IDs
	FBgn0010169
References ( 162 )
Generate a list of	All references relating to this gene ( 162 )
List References by type	Research paper  ( 107 ) Supplementary material  ( 3 ) Review  ( 9 ) Personal communication to FlyBase  ( 20 ) Abstract  ( 12 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Book  ( 2 ) Thesis  ( 1 ) Curated genome annotation  ( 2 ) Teaching note  ( 1 )
Recent research papers ( 3 )
	Clark et al., 2012, PLoS ONE 7(5): e37351 Relative Effectiveness of Mating Success and Sperm Competition at Eliminating Deleterious Mutations in Drosophila melanogaster. [FBrf0218477] Nam et al., 2012, EMBO J. 31(5): 1253--1265 Genetic evidence of a redox-dependent systemic wound response via Hayan Protease-Phenoloxidase system in Drosophila. [FBrf0217593] Tedman-Aucoin and Agrawal, 2012, Evolution 66(2): 575--585 The effect of deleterious mutations and age on recombination in Drosophila melanogaster. [FBrf0217349] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...