FB2020_03, released Jun 16, 2020

Gene: Dmel\Sgs7

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General Information
Symbol
Dmel\Sgs7
Species
D. melanogaster
Name
Salivary gland secretion 7
Annotation Symbol
CG18087
Feature Type
protein_coding_gene
FlyBase ID
FBgn0003377
Gene Model Status
Current
Stock Availability
2 publicly available
Gene Snapshot
Salivary gland secretion 7 (Sgs7) encodes one of the 20-hydroxyecdysone-induced proteins secreted at the larval-to-prepupal transition. They form a glue that serves to attach the prepupa and pupa to a solid substrate. [Date last reviewed: 2019-03-14]
Other Summaries
Also Known As

Sgs-7, group III

Key Links
Genomic Location
Cytogenetic map
68C11-68C11
Sequence location
3L:11,509,861..11,510,247 [+]
Recombination map

3-36

RefSeq locus
NT_037436 REGION:11509861..11510247
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (2 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
puparial adhesion
inferred from expression pattern
(Beckendorf and Kafatos, 1976)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
adhesive extracellular matrix
inferred from direct assay
(Beckendorf and Kafatos, 1976)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Gene Group (FlyBase)
    GLUE PROTEINS -
    Salivary gland secretion genes encode proteins that make up the glue produced by Drosophila larvae that serves to attach the pupa to an external substrate during metamorphosis. (Adapted from FBrf0241323.)
    Phenotypic Description (Red Book; Lindsley and Zimm 1992)
    Sgs7
    Encodes the 5481 kd salivary-gland glue protein, SGS7; the protein contains, in common with SGS3 and SGS8, a 23-residue amino-terminal signal peptide and a carboxy-terminal region of about 50 amino acids that shows considerable homology with that of SGS3 and SGS8; it is not glycosylated. Expressed coordinately with Sgs3 and Sgs8.
    Gene Model and Products
    Number of Transcripts
    1
    Number of Unique Polypeptides
    1

    Please see the GBrowse view of Dmel\Sgs7 or the JBrowse view of Dmel\Sgs7 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model

    Gene model reviewed during 5.43

    Low-frequency RNA-Seq exon junction(s) not annotated.

    Gene model reviewed during 5.45

    Sequence Ontology: Class of Gene
    nuclear_gene
    protein_coding_gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    Sgs7-RA
    FBtr0076095
    NM_057370
    321
    74
    Additional Transcript Data and Comments
    Reported size (kB)

    0.32 (northern blot)

    (Meyerowitz and Hogness, 1982)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    Sgs7-PA
    FBpp0075826
    7.9
    74
    7.91
    NP_476718
    AAF50058
    Polypeptides with Identical Sequences

    There is only one protein coding transcript and one polypeptide associated with this gene

    Additional Polypeptide Data and Comments
    Reported size (kDa)

    74, 51 (aa)

    (Garfinkel et al., 1983)
    Comments
    External Data
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sgs7 using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    northern blot
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    third instar larval stage
    embryonic/larval salivary gland
    (Meyerowitz and Hogness, 1982)
    Additional Descriptive Data

    Sgs3, Sgs7 and Sgs8 are expressed at high levels in salivary glands during the intermolt puff stage.

    (Meyerowitz and Hogness, 1982)
    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    adhesive extracellular matrix
    inferred from direct assay
    (Beckendorf and Kafatos, 1976)
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\Sgs7 in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Bulk Downloads
    RNA-Seq RPKM values for all genes
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Flygut - An atlas of the Drosophila adult midgut
    Images
    FlyBase Wiki
    Image Based Resources
    Alleles, Insertions, and Transgenic Constructs
    Classical and Insertion Alleles ( 1 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 14 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of Sgs7
    Transgenic constructs containing regulatory region of Sgs7
    Deletions and Duplications ( 0 )
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
    viable, with Scer\GAL4Mef2.PR
    viable, with Scer\GAL4pnr-MD237
    Phenotype manifest in
    Allele
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Download All OrthoDB Orthologs
    Human Orthologs (via DIOPT v8.0)
    Homo sapiens (Human) (0)
    No records found.
    Model Organism Orthologs (via DIOPT v8.0)
    Mus musculus (laboratory mouse) (0)
    No records found.
    Rattus norvegicus (Norway rat) (0)
    No records found.
    Xenopus tropicalis (Western clawed frog) (0)
    No records found.
    Danio rerio (Zebrafish) (0)
    No records found.
    Caenorhabditis elegans (Nematode, roundworm) (0)
    No records found.
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190KVM )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila suzukii
    Spotted wing Drosophila
    Dsuz\DS10_00012624
    Drosophila suzukii
    Spotted wing Drosophila
    Dsuz\DS10_00012714
    Drosophila suzukii
    Spotted wing Drosophila
    Dsuz\DS10_00013252
    Drosophila suzukii
    Spotted wing Drosophila
    Dsuz\DS10_00012713
    Drosophila suzukii
    Spotted wing Drosophila
    Dsuz\DS10_00012621
    Drosophila simulans
    Dsim\GD28639
    Drosophila simulans
    Dsim\GD17634
    Drosophila sechellia
    Dsec\GM25278
    Drosophila sechellia
    Dsec\GM24748
    Drosophila erecta
    Dere\Sgs8
    Drosophila erecta
    Dere\GG13918
    Drosophila yakuba
    Dyak\GE21818
    Drosophila yakuba
    Dyak\Sgs8
    Drosophila yakuba
    Dyak\GE20214
    Drosophila pseudoobscura pseudoobscura
    Dpse\GA23426
    Drosophila pseudoobscura pseudoobscura
    Dpse\GA23425
    Drosophila pseudoobscura pseudoobscura
    Dpse\GA23878
    Drosophila persimilis
    Dper\GL22823
    Drosophila willistoni
    Dwil\GK11347
    Drosophila willistoni
    Dwil\GK27355
    Drosophila virilis
    Dvir\GJ26085
    Drosophila virilis
    Dvir\GJ27025
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( None identified )
    No non-Drosophilid orthologies identified
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
    No non-Dipteran orthologies identified
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
    No non-Insect Arthropod orthologies identified
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v8.0)
    Drosophila melanogaster (Fruit fly) (1)
    Gene Symbol
    Score
    Source
    Compara
    eggNOG
    Homologene
    Inparanoid
    Isobase
    OrthoDB
    OrthoFinder
    orthoMCL
    Panther
    RoundUp
    Alignment
    Sgs8
    1 of 10
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Interactions Browser
      Summary of Genetic Interactions
      esyN Network Diagram
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3L
      Recombination map

      3-36

      Cytogenetic map
      68C11-68C11
      Sequence location
      3L:11,509,861..11,510,247 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      68C11-68C11
      Limits computationally determined from genome sequence between P{PZ}l(3)0123901239&P{lacW}l(3)01239j9B4 and P{PZ}CycA02461&P{PZ}CycA03946
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      68C-68C
      (determined by in situ hybridisation)
      (Richards et al., 1983)
      68C3-68C7
      (determined by in situ hybridisation) 68C3--5 (determined by in situ hybridisation)
      (Meyerowitz and Hogness, 1982)
      Associated with the intermolt puff in 68C.
      (Crowley et al., 1983)
      Experimentally Determined Recombination Data
      Location

      3-36

      (FlyBase, 2016)

      3-30.0

      (Comeron et al., 2012)

      3-35.0

      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (2)
      Genomic Clones (11)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (64)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
            Other clones
              RNAi and Array Information
              Linkouts
              DRSC - Results frm RNAi screens
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              Antibody Information
              Laboratory Generated Antibodies
               
              Commercially Available Antibodies
               
              Other Information
              Relationship to Other Genes
              Source for database identify of
              Source for database merge of

              Source for merge of: Sgs7 CG18087

              (Bayraktaroglu, 2000.4.7)
              Additional comments
              Other Comments

              Gene expression is unaffected in Eip74EFneo24 mutants but transcripts are barely detectable in Eip74EFDL-1 mutants.

              (Fletcher and Thummel, 1995)

              Ecdysteroid-regulated gene.

              (Andres et al., 1993)

              Sgs7 encodes the 5481kD salivary-gland glue protein. One of a group of seven genes encoding proteins that are components of the secretion produced by the larval salivary glands during the third instar for the purpose of attaching the larva to the substrate preparative to pupariation. The protein contains, in common with those encoded by Sgs3 and Sgs8, a 23-residue amino-terminal signal peptide and a carboxy-terminal region of about 50 amino acids that shows considerable homology with those encoded by Sgs3 and Sgs8; it is not glycosylated. Expressed coordinately with Sgs3 and Sgs8.

              (Lindsley and Zimm, 1992)

              A cis-acting element required for the correct expression of both Sgs7 and Sgs8 is located asymmetrically in the intergenic region between the two genes.

              (Hofmann et al., 1991)

              Cessation of Sgs7 transcription and puff regression are ecdysterone-dependent.

              (Crowley and Meyerowitz, 1984)

              Initiation of transcription of Sgs7, but not of intermolt-puff formation seems to depend on the presence of suitable levels of ecdysterone in early third instar larvae.

              (Hansson and Lambertsson, 1983)

              Identification: During a molecular analysis of the 68C puff locus.

              (Meyerowitz and Hogness, 1982)

              Sgs7 and Sgs8 are oppositely oriented so that their promoters are adjacent, suggesting that this pair may form a single regulated unit. The pair is bounded by an inverted repeat of 0.3kb elements.

              (Meyerowitz and Hogness, 1982)

              Sequences located 5' to Sgs7, Sgs8, Sgs3, the Hsp70 genes at 87A and 87C and the copia coding region are similar to the sequence at -405 from Sgs4.

              (Shermoen and Beckendorf, 1982)

              Synthesis of the glue proteins begins about 106 h after egg deposition and ceases abruptly within a few minutes after the glue is released 14 h later.

              (Beckendorf and Kafatos, 1976)

              Initiation of transcription of Sgs7 is coincident with the formation of the intermolt puffs in early to mid third instar.

              (Korge, 1975)
              Origin and Etymology
              Discoverer
              Etymology
              Identification
              External Crossreferences and Linkouts ( 24 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
              Other crossreferences
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              Flygut - An atlas of the Drosophila adult midgut
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              KEGG Genes - Molecular building blocks of life in the genomic space.
              modMine - A data warehouse for the modENCODE project
              Linkouts
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              FLIGHT - Cell culture data for RNAi and other high-throughput technologies
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
              FlyMine - An integrated database for Drosophila genomics
              Synonyms and Secondary IDs (9)
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              References (73)