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Gene Dmel\Sgs8

General Information
Symbol	Dmel\Sgs8	Species	D. melanogaster
Name	Salivary gland secretion 8	Annotation symbol	CG6132
Feature type	protein_coding_gene	FlyBase ID	FBgn0003378
Gene Model Status	Current	Stock availability	2 publicly available
Also Known As	Sgs-8
Genomic Location
Chromosome (arm)	3L	Recombination map	3-35.0
Cytogenetic map	68C11-68C11	Sequence location	3L:11,502,067..11,502,494 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Salivary gland secretion 8 is referred to in FlyBase by the symbol Dmel\Sgs8 (CG6132, FBgn0003378). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: structural molecule activity. It is reported to be involved in the biological process: puparial adhesion. One allele is reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of no expression detected. Peak expression observed during late larval stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval fat body, larval salivary gland. Comments on Affy2 ProbeSet: ProbeSet 1638108_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Sgs8. Gene sequence location is 3L:11502067..11502494.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Sgs8 Encodes the 5592 kd salivary-gland glue protein, SGS7; the protein contains, in common with SGS3 and SGS7, a 23-residue amino-terminal signal peptide and a carboxy-terminal region of about 50 amino acids that shows considerable homology with that of SGS3 and SGS8; it is not glycosylated. Expressed coordinately with Sgs3 and Sgs7.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 68C11-68C11   Limits computationally determined from genome sequence between P{PZ}l(3)0123901239&P{lacW}l(3)01239j9B4 and P{PZ}CycA02461&P{PZ}CycA03946
Experimentally Determined Cytological Location
Cytogenetic map Notes References 68C-68C   (determined by in situ hybridisation)   (Martin and Meyerowitz, 1986) 68C3-68C7   (determined by in situ hybridisation) 68C3--5 (determined by in situ hybridisation)   (Meyerowitz and Hogness, 1982)   Associated with the intermolt puff in 68C.   (Crowley et al., 1983)
Experimentally Determined Recombination Data
Location	3-35.0
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Sgs8 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43 Gene model reviewed during 5.45
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Sgs8-RA FBtr0076160  NM_057371   359   75
Additional Transcript Data & Comments
Reported size (kB)	0.36 (unknown) (Garfinkel et al., 1983) 0.36 (northern blot) (Meyerowitz and Hogness, 1982)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Sgs8-PA   FBpp0075891   7.9   75   4.83     NP_476719   AAF50059
Additional Polypeptide Data & Comments
Reported size (kDa)	74, 52 (aa) (Garfinkel et al., 1983)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF50059   AC004438   BT053728   CA803347   CK131854   CO179808   CO179829   CO180457   CO180710   CO181120   CO181254   CO181565   CO182173   CO182211   CO182508   CO182743   CO182899   CO184122   CO184656   CO184770   CO184987   CO185039   CO185057   CO185083   CO191126   CO191217   CO191453   CO192580   CO192771   CO193340   CO193385   CO193622   CO193669   CO193756   CO193762   CO194600   CO194752   CO195253   CO195627   CO195649   CO269795   CO294698   EC063914   EC071330   EC233526   EL886908   L80121   L80122   L80123   M14650   X01918 CAA25992
UniProtKB/Swiss-Prot	P02842
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References regulatory region FBsf0000161157 3L:11,502,302..11,502,887 evidence=experimental comment=region sufficient to confer Sgs8 expression pattern on reporter fusion construct (Hofmann et al., 1991) regulatory region FBsf0000161205 3L:11,502,302..11,502,986 comment=region sufficient for normal regulation by br evidence=experimental (Hofmann et al., 1991) regulatory region FBsf0000435291 3L:11,502,159..11,502,844 evidence=Reporter construct (in vivo) (Hofmann et al., 1991, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences	EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters • 11022 11023
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   salivary gland   (Meyerowitz and Hogness, 1982)
Additional Descriptive Data
	Sgs3, Sgs7 and Sgs8 are expressed at high levels in salivary glands during the intermolt puff stage. (Meyerowitz and Hogness, 1982)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval fat body, larval salivary gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    4.1 Larval Midgut    6 Larval Hindgut    6.7 Larval Malpighian Tubules    3.7 Larval Fat Body    2233.1 Larval Salivary Gland    5834.8 Larval Trachea    6.725 Larval Carcass    8.575 Adult Head    3.4 Adult Eye    4.325 Adult Brain    2.8 Adult Thoracic-Abdominal Ganglion    3.9 Adult Crop    4.5 Adult Midgut    5.9 Adult Hindgut    6 Adult Malpighian Tubules    4.6 Adult Fat Body    19.6 Adult Salivary Gland    2 Adult Heart    2.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    7.5 Adult Ovary    2.7 Adult Testis    2.5 Adult Male Accessory Gland    2.1 Adult Carcass    6.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    4.1 Larval Midgut    6 Larval Hindgut    6.7 Larval Malpighian Tubules    3.7 Larval Fat Body  (2233.1) Larval Salivary Gland  (5834.8) Larval Trachea    6.725 Larval Carcass    8.575 Adult Head    3.4 Adult Eye    4.325 Adult Brain    2.8 Adult Thoracic-Abdominal Ganglion    3.9 Adult Crop    4.5 Adult Midgut    5.9 Adult Hindgut    6 Adult Malpighian Tubules    4.6 Adult Fat Body    19.6 Adult Salivary Gland    2 Adult Heart    2.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    7.5 Adult Ovary    2.7 Adult Testis    2.5 Adult Male Accessory Gland    2.1 Adult Carcass    6.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    4.1 Larval Midgut    6 Larval Hindgut    6.7 Larval Malpighian Tubules    3.7 Larval Fat Body  (2233.1) Larval Salivary Gland  (5834.8) Larval Trachea    6.725 Larval Carcass    8.575 Adult Head    3.4 Adult Eye    4.325 Adult Brain    2.8 Adult Thoracic-Abdominal Ganglion    3.9 Adult Crop    4.5 Adult Midgut    5.9 Adult Hindgut    6 Adult Malpighian Tubules    4.6 Adult Fat Body    19.6 Adult Salivary Gland    2 Adult Heart    2.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    7.5 Adult Ovary    2.7 Adult Testis    2.5 Adult Male Accessory Gland    2.1 Adult Carcass    6.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    4.1 Larval Midgut    6 Larval Hindgut    6.7 Larval Malpighian Tubules    3.7 Larval Fat Body    2233.1 Larval Salivary Gland    5834.8 Larval Trachea    6.725 Larval Carcass    8.575 Adult Head    3.4 Adult Eye    4.325 Adult Brain    2.8 Adult Thoracic-Abdominal Ganglion    3.9 Adult Crop    4.5 Adult Midgut    5.9 Adult Hindgut    6 Adult Malpighian Tubules    4.6 Adult Fat Body    19.6 Adult Salivary Gland    2 Adult Heart    2.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    7.5 Adult Ovary    2.7 Adult Testis    2.5 Adult Male Accessory Gland    2.1 Adult Carcass    6.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    4.1 Larval Midgut    6 Larval Hindgut    6.7 Larval Malpighian Tubules    3.7 Larval Fat Body    2233.1 Larval Salivary Gland    5834.8 Larval Trachea    6.725 Larval Carcass    8.575 Adult Head    3.4 Adult Eye    4.325 Adult Brain    2.8 Adult Thoracic-Abdominal Ganglion    3.9 Adult Crop    4.5 Adult Midgut    5.9 Adult Hindgut    6 Adult Malpighian Tubules    4.6 Adult Fat Body    19.6 Adult Salivary Gland    2 Adult Heart    2.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    7.5 Adult Ovary    2.7 Adult Testis    2.5 Adult Male Accessory Gland    2.1 Adult Carcass    6.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    4.1 Larval Midgut    6 Larval Hindgut    6.7 Larval Malpighian Tubules    3.7 Larval Fat Body  (2233.1) Larval Salivary Gland  (5834.8) Larval Trachea    6.725 Larval Carcass    8.575 Adult Head    3.4 Adult Eye    4.325 Adult Brain    2.8 Adult Thoracic-Abdominal Ganglion    3.9 Adult Crop    4.5 Adult Midgut    5.9 Adult Hindgut    6 Adult Malpighian Tubules    4.6 Adult Fat Body    19.6 Adult Salivary Gland    2 Adult Heart    2.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    7.5 Adult Ovary    2.7 Adult Testis    2.5 Adult Male Accessory Gland    2.1 Adult Carcass    6.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    4.1 Larval Midgut    6 Larval Hindgut    6.7 Larval Malpighian Tubules    3.7 Larval Fat Body  (2233.1) Larval Salivary Gland  (5834.8) Larval Trachea    6.725 Larval Carcass    8.575 Adult Head    3.4 Adult Eye    4.325 Adult Brain    2.8 Adult Thoracic-Abdominal Ganglion    3.9 Adult Crop    4.5 Adult Midgut    5.9 Adult Hindgut    6 Adult Malpighian Tubules    4.6 Adult Fat Body    19.6 Adult Salivary Gland    2 Adult Heart    2.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    7.5 Adult Ovary    2.7 Adult Testis    2.5 Adult Male Accessory Gland    2.1 Adult Carcass    6.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    4.1 Larval Midgut    6 Larval Hindgut    6.7 Larval Malpighian Tubules    3.7 Larval Fat Body    2233.1 Larval Salivary Gland    5834.8 Larval Trachea    6.725 Larval Carcass    8.575 Adult Head    3.4 Adult Eye    4.325 Adult Brain    2.8 Adult Thoracic-Abdominal Ganglion    3.9 Adult Crop    4.5 Adult Midgut    5.9 Adult Hindgut    6 Adult Malpighian Tubules    4.6 Adult Fat Body    19.6 Adult Salivary Gland    2 Adult Heart    2.45 Adult VirginFemale Spermatheca    4.6 Adult InseminatedFemale Spermatheca    7.5 Adult Ovary    2.7 Adult Testis    2.5 Adult Male Accessory Gland    2.1 Adult Carcass    6.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    3.4   NA  Eye    4.325   NA  Brain    2.8   4.1  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    3.9   NA  Crop    4.5   6  Midgut    5.9   6.7  Hindgut    6   3.7  Malpighian Tubules    4.6   2233.1  Fat Body    no informative data   5834.8  Salivary Gland    2   NA  Heart    2.45   6.725  Trachea    NA   NA  VirginFemale Spermatheca    4.6   NA  InseminatedFemale Spermatheca    7.5   NA  Ovary    2.7   NA  Testis    2.5   NA  Male Accessory Gland    2.1   8.575  Carcass    6.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    531 central nervous system, larvae L3    67 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering    18730 salivary gland, white prepupae    322 digestive system, larvae L3 wandering    80 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering    58 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    8 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (531) central nervous system, larvae L3  (67) central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering  (18730) salivary gland, white prepupae  (322) digestive system, larvae L3 wandering  (80) digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering  (58) carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    8 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (531) central nervous system, larvae L3    67 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering  (18730) salivary gland, white prepupae  (322) digestive system, larvae L3 wandering    80 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering    58 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    8 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    531 central nervous system, larvae L3    67 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering    18730 salivary gland, white prepupae    322 digestive system, larvae L3 wandering    80 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering    58 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    8 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    531 central nervous system, larvae L3    67 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering    18730 salivary gland, white prepupae    322 digestive system, larvae L3 wandering    80 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering    58 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    8 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (531) central nervous system, larvae L3  (67) central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering  (18730) salivary gland, white prepupae  (322) digestive system, larvae L3 wandering  (80) digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering  (58) carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    8 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (531) central nervous system, larvae L3    67 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering  (18730) salivary gland, white prepupae  (322) digestive system, larvae L3 wandering    80 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering    58 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    8 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    531 central nervous system, larvae L3    67 central nervous system, pupae P8    2 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering    18730 salivary gland, white prepupae    322 digestive system, larvae L3 wandering    80 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering    58 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    8 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of no expression detected. Peak expression observed during late larval stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    3256 larva L3 puffstage 3-6    6499 larva L3 puffstage 7-9    5192 white prepupae new    603 white prepupae 12hr    6 white prepupae 24hr    1 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2  (3256) larva L3 puffstage 3-6  (6499) larva L3 puffstage 7-9  (5192) white prepupae new  (603) white prepupae 12hr    6 white prepupae 24hr    1 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2  (3256) larva L3 puffstage 3-6  (6499) larva L3 puffstage 7-9  (5192) white prepupae new  (603) white prepupae 12hr    6 white prepupae 24hr    1 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    3256 larva L3 puffstage 3-6    6499 larva L3 puffstage 7-9    5192 white prepupae new    603 white prepupae 12hr    6 white prepupae 24hr    1 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    3256 larva L3 puffstage 3-6    6499 larva L3 puffstage 7-9    5192 white prepupae new    603 white prepupae 12hr    6 white prepupae 24hr    1 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2  (3256) larva L3 puffstage 3-6  (6499) larva L3 puffstage 7-9  (5192) white prepupae new  (603) white prepupae 12hr    6 white prepupae 24hr    1 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2  (3256) larva L3 puffstage 3-6  (6499) larva L3 puffstage 7-9  (5192) white prepupae new  (603) white prepupae 12hr    6 white prepupae 24hr    1 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    3256 larva L3 puffstage 3-6    6499 larva L3 puffstage 7-9    5192 white prepupae new    603 white prepupae 12hr    6 white prepupae 24hr    1 pupae 2d postWPP    1 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    545 cold shock, 4-day adult    812 heat shock, 4-day adult    147 Cadmium 50 mM 6 hrs, larvae L3    453 Cadmium 50 mM 12 hrs, larvae L3    1353 Cadmium 50 mM 48 hrs, 4-day adult    106 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    228 Copper 15 mM 48 hrs, 4-day adult    49 Zinc 5 mM 12 hrs, larvae L3    293 Zinc 4.5 mM 48 hrs, 4-day adult    125 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    199 Ethanol 10% 3 hrs, larvae L3    305 Caffeine 1.5 mg/ml 4 hrs, larvae L3    383 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    627 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    350 Rotenone 8 μg 12 hrs, larvae L3    71 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (545) cold shock, 4-day adult  (812) heat shock, 4-day adult  (147) Cadmium 50 mM 6 hrs, larvae L3  (453) Cadmium 50 mM 12 hrs, larvae L3  (1353) Cadmium 50 mM 48 hrs, 4-day adult  (106) Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3  (228) Copper 15 mM 48 hrs, 4-day adult  (49) Zinc 5 mM 12 hrs, larvae L3  (293) Zinc 4.5 mM 48 hrs, 4-day adult  (125) Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3  (199) Ethanol 10% 3 hrs, larvae L3  (305) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (383) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult  (627) Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3  (350) Rotenone 8 μg 12 hrs, larvae L3  (71) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (545) cold shock, 4-day adult  (812) heat shock, 4-day adult  (147) Cadmium 50 mM 6 hrs, larvae L3  (453) Cadmium 50 mM 12 hrs, larvae L3  (1353) Cadmium 50 mM 48 hrs, 4-day adult  106 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3  (228) Copper 15 mM 48 hrs, 4-day adult    49 Zinc 5 mM 12 hrs, larvae L3  (293) Zinc 4.5 mM 48 hrs, 4-day adult  (125) Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3  (199) Ethanol 10% 3 hrs, larvae L3  (305) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (383) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult  (627) Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3  (350) Rotenone 8 μg 12 hrs, larvae L3    71 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    545 cold shock, 4-day adult    812 heat shock, 4-day adult    147 Cadmium 50 mM 6 hrs, larvae L3    453 Cadmium 50 mM 12 hrs, larvae L3    1353 Cadmium 50 mM 48 hrs, 4-day adult    106 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    228 Copper 15 mM 48 hrs, 4-day adult    49 Zinc 5 mM 12 hrs, larvae L3    293 Zinc 4.5 mM 48 hrs, 4-day adult    125 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    199 Ethanol 10% 3 hrs, larvae L3    305 Caffeine 1.5 mg/ml 4 hrs, larvae L3    383 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    627 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    350 Rotenone 8 μg 12 hrs, larvae L3    71 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    545 cold shock, 4-day adult    812 heat shock, 4-day adult    147 Cadmium 50 mM 6 hrs, larvae L3    453 Cadmium 50 mM 12 hrs, larvae L3    1353 Cadmium 50 mM 48 hrs, 4-day adult    106 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    228 Copper 15 mM 48 hrs, 4-day adult    49 Zinc 5 mM 12 hrs, larvae L3    293 Zinc 4.5 mM 48 hrs, 4-day adult    125 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    199 Ethanol 10% 3 hrs, larvae L3    305 Caffeine 1.5 mg/ml 4 hrs, larvae L3    383 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    627 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    350 Rotenone 8 μg 12 hrs, larvae L3    71 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (545) cold shock, 4-day adult  (812) heat shock, 4-day adult  (147) Cadmium 50 mM 6 hrs, larvae L3  (453) Cadmium 50 mM 12 hrs, larvae L3  (1353) Cadmium 50 mM 48 hrs, 4-day adult  (106) Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3  (228) Copper 15 mM 48 hrs, 4-day adult  (49) Zinc 5 mM 12 hrs, larvae L3  (293) Zinc 4.5 mM 48 hrs, 4-day adult  (125) Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3  (199) Ethanol 10% 3 hrs, larvae L3  (305) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (383) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult  (627) Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3  (350) Rotenone 8 μg 12 hrs, larvae L3  (71) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (545) cold shock, 4-day adult  (812) heat shock, 4-day adult  (147) Cadmium 50 mM 6 hrs, larvae L3  (453) Cadmium 50 mM 12 hrs, larvae L3  (1353) Cadmium 50 mM 48 hrs, 4-day adult  106 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3  (228) Copper 15 mM 48 hrs, 4-day adult    49 Zinc 5 mM 12 hrs, larvae L3  (293) Zinc 4.5 mM 48 hrs, 4-day adult  125 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3  (199) Ethanol 10% 3 hrs, larvae L3  (305) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (383) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult  (627) Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3  (350) Rotenone 8 μg 12 hrs, larvae L3    71 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    545 cold shock, 4-day adult    812 heat shock, 4-day adult    147 Cadmium 50 mM 6 hrs, larvae L3    453 Cadmium 50 mM 12 hrs, larvae L3    1353 Cadmium 50 mM 48 hrs, 4-day adult    106 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    228 Copper 15 mM 48 hrs, 4-day adult    49 Zinc 5 mM 12 hrs, larvae L3    293 Zinc 4.5 mM 48 hrs, 4-day adult    125 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    199 Ethanol 10% 3 hrs, larvae L3    305 Caffeine 1.5 mg/ml 4 hrs, larvae L3    383 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    627 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    350 Rotenone 8 μg 12 hrs, larvae L3    71 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_32
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003378 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6132-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Sgs8GD13604 viable, with Scer\GAL4pnr-MD237 Sgs8GD13604 Phenotype manifest in Allele embryonic/larval brain | larval stage Sgs8GD13604
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of Sgs8 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Sgs8 Class Mutagen Stocks Known lesion Sgs8GD13604 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data reporter construct P{GGAX-7} No P{GGAX-8} No P{GGAX-88} No P{GLX0.66} No P{GLX0.68} No P{GLX125} No P{GLX226} No UAS construct P{GD13604} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
structural molecule activity traceable author statement (Lindsley and Zimm, 1992)
Biological Process
CV term Evidence References
puparial adhesion traceable author statement (Lindsley and Zimm, 1992)
Cellular Component
CV term Evidence References
extracellular region traceable author statement (Lindsley and Zimm, 1992)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Sgs8 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0003378
Orthologs
OrthoDB Orthologs (15) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6ZPFG6 Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6ZPFG6)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Sgs7     Drosophila melanogaster fruit fly  Dmel\Sgs8     Drosophila simulans   Dsim\GD17634   Y Drosophila sechellia   Dsec\GM25278   Y Drosophila sechellia   Dsec\GM24748 Y Y Drosophila erecta   Dere\Sgs8 Y Y Drosophila erecta   Dere\GG13918   Y Drosophila yakuba   Dyak\GE21818   Y Drosophila yakuba   Dyak\Sgs8 Y Y Drosophila yakuba   Dyak\GE20214   Y Drosophila ananassae   Dana\GF25269 Y Y Drosophila pseudoobscura pseudoobscura   Dpse\GA23426 Y Y Drosophila pseudoobscura pseudoobscura   Dpse\GA23425   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA23878   Y Drosophila persimilis   Dper\GL22823   Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (5) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM24748 Drosophila erecta Dere\Sgs8 Drosophila yakuba Dyak\Sgs8 Drosophila ananassae Dana\GF25269 Drosophila pseudoobscura pseudoobscura Dpse\GA23426
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
VDRC	v23526 w1118; P{GD13604}v23526/TM3 v23527 w1118; P{GD13604}v23527/CyO
Genomic Clones ( 1 )
	BACR03I05 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 44 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LP14045
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LP12881
Other clones	35237 75386 CK01.129C.B2 EC00242 EC00288 EC01581 EC03361 EC13573 EC13892 EC14555 EC22208 EC22271 EC22892 EC23863 EC24239 EC27180 EC28376 EC28546 EC29041 EC29213 EC29247 EC29292 EC30163 EC30462 EC30887 EC34102 EC34463 EC35624 EC35691 EC36507 EC36602 EC36740 EC36751 EC38880 EC39165 EC39932 EC40692 EC40725 EK095937 EK207464 ESG011c.E7_G11 IP18121
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003378 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39285
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Sgs8 CG6132 (Zhang, 1999.11)
Source for database merge of
Additional comments
Other Comments
	Gene expression is unaffected in Eip74EFneo24 mutants but transcripts are barely detectable in Eip74EFDL-1 mutants. (Fletcher and Thummel, 1995) Ecdysteroid-regulated gene. (Andres et al., 1993) Encodes a 5592kD salivary-gland glue protein. One of a group of seven genes encoding proteins that are components of the secretion produced by the larval salivary glands during the third instar for the purpose of attaching the larva to the substrate preparative to pupariation. (Lindsley and Zimm, 1992) A cis-acting element required for the correct expression of both Sgs7 and Sgs8 is located asymmetrically in the intergenic region between the two genes. (Hofmann et al., 1991) Cessation of Sgs8 transcription and puff regression are ecdysterone-dependent. (Crowley and Meyerowitz, 1984) Initiation of transcription of Sgs8, but not of intermolt-puff formation seems to depend on the presence of suitable levels of ecdysterone in early third instar larvae. (Hansson and Lambertsson, 1983) Identification: During a molecular analysis of the 68C puff locus. (Meyerowitz and Hogness, 1982) Sgs7 and Sgs8 are oppositely oriented so that their promoters are adjacent, suggesting that this pair may form a single regulated unit. The pair is bounded by an inverted repeat of 0.3kb elements. (Meyerowitz and Hogness, 1982) Sequences located 5' to Sgs7, Sgs8, Sgs3, the Hsp70 genes at 87A and 87C and the copia coding region are similar to the sequence at -405 from Sgs4. (Shermoen and Beckendorf, 1982) Synthesis of the glue proteins begins about 106 h after egg deposition and ceases abruptly within a few minutes after the glue is released 14 h later. (Beckendorf and Kafatos, 1976) Initiation of transcription of Sgs8 is coincident with the formation of the intermolt puffs in early to mid third instar. (Korge, 1975)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057371 RefSeq (Proteins) • NP_476719 DDBJ / EMBL / GenBank • AAF50059 AC004438 CAA25992 L80121 L80122 L80123 M14650 X01918 Entrez Gene - A searchable database of RefSeq genes. • 39285 UniProtKB/Swiss-Prot • P02842
Other Crossreferences
	EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters • 11022 11023
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0003378 DRSC - Results from RNAi screens. • FBgn0003378 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003378 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6132-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003378 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39285 modMine - Data generated by the modENCODE project. • FBgn0003378
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	68C GII (Snyder et al., 1982) CG6132 (Tang et al., 2010, Cammarato et al., 2011) Sgs (Prohaska et al., 2003) sgs8 (Li and White, 2003) Sgs-8 (Kaplan et al., 2000, White et al., 1999, Lehmann, 1996, Fletcher and Thummel, 1995, Andres and Thummel, 1992, Hofmann et al., 1991, Swida et al., 1990, Barnett et al., 1990, Hansson and Lambertsson, 1989, Martin et al., 1989, Mettling et al., 1988, Jongens et al., 1988, Giangrande et al., 1987, Meyerowitz and Martin, 1984, Crowley and Meyerowitz, 1984, Beckstead et al., 2005) sgs-8 (Garfinkel, 1995.8.22, Lanio et al., 1994, Carulli et al., 1993, Crowley et al., 1983) Sgs8 (Reiter et al., 2007, Zhai et al., 2012, Weake et al., 2008, Costantino et al., 2008, Jin et al., 2009, Liu and Lehmann, 2008, Blanco et al., 2008, Palomera-Sanchez et al., 2010, Tang et al., 2010)
Name Synonym	Gene II (Meyerowitz and Hogness, 1982) group II (Shermoen and Beckendorf, 1982, ) Salivary gland secretion 8 (Cammarato et al., 2011)
Secondary FlyBase IDs
References ( 62 )
Generate a list of	All references relating to this gene ( 62 )
List References by type	Research paper  ( 43 ) Supplementary material  ( 4 ) Review  ( 3 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 1 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 3 ) Book  ( 1 )
Recent research papers ( 1 )
	Zhai et al., 2012, PLoS Genet. 8(3): e1002582 Antagonistic regulation of apoptosis and differentiation by the cut transcription factor represents a tumor-suppressing mechanism in Drosophila. [FBrf0217859] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...