FlyBase Gene Report: Dmel\Sgs8

General Information

Symbol

Dmel\Sgs8

Species

D. melanogaster

Name

Salivary gland secretion 8

Annotation Symbol

CG6132

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003378

Gene Model Status

Current

Stock Availability

3 publicly available

Gene Summary

Salivary gland secretion 8 (Sgs8) encodes one of the 20-hydroxyecdysone-induced proteins secreted at the larval-to-prepupal transition. They form a glue that serves to attach the prepupa and pupa to a solid substrate. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

Sgs-8, group II

Key Links

Genomic Location

Cytogenetic map

68C11-68C11

Sequence location

3L:11,508,967..11,509,394 [-]

Recombination map

3-36

RefSeq locus

NT_037436 REGION:11508967..11509394

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in puparial adhesion

inferred from expression pattern

(Beckendorf and Kafatos, 1976)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in adhesive extracellular matrix

inferred from direct assay

(Beckendorf and Kafatos, 1976)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

GLUE PROTEINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

GLUE PROTEINS -: Salivary gland secretion genes encode proteins that make up the glue produced by Drosophila larvae that serves to attach the pupa to an external substrate during metamorphosis. (Adapted from FBrf0241323.)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Sgs8

Encodes the 5592 kd salivary-gland glue protein, SGS7; the protein contains, in common with SGS3 and SGS7, a 23-residue amino-terminal signal peptide and a carboxy-terminal region of about 50 amino acids that shows considerable homology with that of SGS3 and SGS8; it is not glycosylated. Expressed coordinately with Sgs3 and Sgs7.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Sgs8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P02842)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.43

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Sgs8-RA

FBtr0076160

NM_057371

359

Additional Transcript Data and Comments

Reported size (kB)

0.36 (unknown)

(Garfinkel et al., 1983)

0.36 (northern blot)

(Meyerowitz and Hogness, 1982)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Sgs8-PA

FBpp0075891

7.9

4.83

P02842

NP_476719

AAF50059

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

74, 52 (aa)

(Garfinkel et al., 1983)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sgs8 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Sgs8_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.19

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

salivary gland

(Meyerowitz and Hogness, 1982)

Additional Descriptive Data

Sgs3, Sgs7 and Sgs8 are expressed at high levels in salivary glands during the intermolt puff stage.

(Meyerowitz and Hogness, 1982)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in adhesive extracellular matrix

inferred from direct assay

(Beckendorf and Kafatos, 1976)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Sgs8 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Sgs8

View exonic expression by tissue for Dmel\Sgs8

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003378

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39285/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003378

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003378

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003378

Flygut - An atlas of the Drosophila adult midgut

FBgn0003378

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Sgs8

Transgenic constructs containing regulatory region of Sgs8

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^insc-Mz1407

Sgs8^GD13604

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Sgs8^GD13604

viable, with Scer\GAL4^Mef2.PR

Sgs8^GD13604

viable, with Scer\GAL4^pnr-MD237

Sgs8^GD13604

Phenotype manifest in

Allele

larval brain | larval stage, with Scer\GAL4^insc-Mz1407

Sgs8^GD13604

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (0)

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Sgs8. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

Sgs7

2 of 13

Sgs3

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0003378

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-36

Cytogenetic map

68C11-68C11

Sequence location

3L:11,508,967..11,509,394 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

68C11-68C11

Limits computationally determined from genome sequence between P{PZ}l(3)01239⁰¹²³⁹&P{lacW}l(3)01239^j9B4 and P{PZ}CycA⁰²⁴⁶¹&P{PZ}CycA⁰³⁹⁴⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

68C-68C

(determined by in situ hybridisation)

(Martin and Meyerowitz, 1986)

68C3-68C7

(determined by in situ hybridisation) 68C3--5 (determined by in situ hybridisation)

(Meyerowitz and Hogness, 1982)

Associated with the intermolt puff in 68C.

(Crowley et al., 1983)

Experimentally Determined Recombination Data

Location

3-36

(FlyBase, 2016)

3-30.0

(Comeron et al., 2012)

3-35.0

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

65165

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05953}attP40/CyO

VDRC

v23526

w¹¹¹⁸; P{GD13604}v23526/TM3

v23527

w¹¹¹⁸; P{GD13604}v23527/CyO

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (34)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LP14045

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003378

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

LP12881

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003378

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Gene expression is unaffected in Eip74EF^neo24 mutants but transcripts are barely detectable in Eip74EF^DL-1 mutants.

(Fletcher and Thummel, 1995)

Ecdysteroid-regulated gene.

(Andres et al., 1993)

Encodes a 5592kD salivary-gland glue protein. One of a group of seven genes encoding proteins that are components of the secretion produced by the larval salivary glands during the third instar for the purpose of attaching the larva to the substrate preparative to pupariation.

(Lindsley and Zimm, 1992)

A cis-acting element required for the correct expression of both Sgs7 and Sgs8 is located asymmetrically in the intergenic region between the two genes.

(Hofmann et al., 1991)

Cessation of Sgs8 transcription and puff regression are ecdysterone-dependent.

(Crowley and Meyerowitz, 1984)

Initiation of transcription of Sgs8, but not of intermolt-puff formation seems to depend on the presence of suitable levels of ecdysterone in early third instar larvae.

(Hansson and Lambertsson, 1983)

Identification: During a molecular analysis of the 68C puff locus.

(Meyerowitz and Hogness, 1982)

Sgs7 and Sgs8 are oppositely oriented so that their promoters are adjacent, suggesting that this pair may form a single regulated unit. The pair is bounded by an inverted repeat of 0.3kb elements.

(Meyerowitz and Hogness, 1982)

Sequences located 5' to Sgs7, Sgs8, Sgs3, the Hsp70 genes at 87A and 87C and the copia coding region are similar to the sequence at -405 from Sgs4.

(Shermoen and Beckendorf, 1982)

Synthesis of the glue proteins begins about 106 h after egg deposition and ceases abruptly within a few minutes after the glue is released 14 h later.

(Beckendorf and Kafatos, 1976)

Initiation of transcription of Sgs8 is coincident with the formation of the intermolt puffs in early to mid third instar.

(Korge, 1975)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Sgs8 CG6132

(Zhang, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

68C GII

(Snyder et al., 1982)

CG6132

(Chen et al., 2012, Cammarato et al., 2011, Tang et al., 2010, Zhang et al., 2010, Perkins et al., 2009)

Sgs

(Prohaska et al., 2003)

Sgs-8

(Beckstead et al., 2005, Kaplan et al., 2000, White et al., 1999, Lehmann, 1996, Fletcher and Thummel, 1995, Andres and Thummel, 1992, Hofmann et al., 1991, Barnett et al., 1990, Swida et al., 1990, Hansson and Lambertsson, 1989, Martin et al., 1989, Jongens et al., 1988, Mettling et al., 1988, Giangrande et al., 1987, Crowley and Meyerowitz, 1984, Meyerowitz and Martin, 1984)

sgs-8

(Garfinkel, 1995.8.22, Lanio et al., 1994, Carulli et al., 1993, Crowley et al., 1983)

sgs8

(Li and White, 2003)

Name Synonyms

Gene II

(Meyerowitz and Hogness, 1982)

Salivary gland secretion 8

(Cammarato et al., 2011)

group II

(Shermoen and Beckendorf, 1982)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 28 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39285

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P02842

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P02842

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P02842

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003378

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39285/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003378

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003378

FlyMine - An integrated database for Drosophila genomics

FBgn0003378

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6132

MARRVEL_MODEL - MARRVEL (model organism gene)

39285

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003378

DroID - A comprehensive database of gene and protein interactions.

FBgn0003378

DRSC - Results frm RNAi screens

FBgn0003378

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Sgs8_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003378

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003378

Flygut - An atlas of the Drosophila adult midgut

FBgn0003378

References (87)

The following external sites may use different assemblies or annotations than FlyBase.

-0.19

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