FlyBase Gene Report: Dmel\shd

General Information

Symbol

Dmel\shd

Species

D. melanogaster

Name

shade

Annotation Symbol

CG13478

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003388

Gene Model Status

Stock Availability

6 publicly available

Enzyme Name (EC)

Ecdysone 20-monooxygenase (1.14.99.22)

Gene Summary

shade (shd) encodes 20-hydroxylase and is responsible for converting Ecdysone into 20-hydroxyecdysone, the active form of the steroid. It is required in all tissues that produce active Ecdysone and thus contributes to larval moulting, metamorphosis, growth, neuroblast diversity and egg chamber maturation. [Date last reviewed: 2018-09-13] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

Cyp314a1

Key Links

Genomic Location

Cytogenetic map

Sequence location

3L:14,614,458..14,622,205 [+]

Recombination map

3-42

RefSeq locus

NT_037436 REGION:14614458..14622205

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables ecdysone 20-monooxygenase activity

inferred from direct assay

(Petryk et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables heme binding

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables iron ion binding

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (8 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in border follicle cell migration

inferred from mutant phenotype

(Domanitskaya et al., 2014)

involved_in central nervous system development

inferred from mutant phenotype

(Giesen et al., 2003)

involved_in dorsal closure

inferred from mutant phenotype

(Giesen et al., 2003, Petryk et al., 2003)

involved_in ecdysteroid biosynthetic process

inferred from direct assay

(Petryk et al., 2003)

inferred from mutant phenotype

(Petryk et al., 2003)

involved_in growth of a germarium-derived egg chamber

inferred from mutant phenotype

(Petryk et al., 2003)

involved_in head involution

inferred from mutant phenotype

(Giesen et al., 2003)

involved_in midgut development

inferred from mutant phenotype

(Petryk et al., 2003)

involved_in pupation

inferred from mutant phenotype

(Akagi et al., 2016)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in mitochondrion

inferred from direct assay

(Petryk et al., 2003)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

CYTOCHROME P450 - MITO CLAN

Protein Family (UniProt)

Belongs to the cytochrome P450 family. (Q9VUF8)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

Ecdysone + AH(2) + O(2) = 20-hydroxyecdysone + A + H(2)O (1.14.99.22)

Predictions / Assertions

-

Summaries

Gene Snapshot

shade (shd) encodes 20-hydroxylase and is responsible for converting Ecdysone into 20-hydroxyecdysone, the active form of the steroid. It is required in all tissues that produce active Ecdysone and thus contributes to larval moulting, metamorphosis, growth, neuroblast diversity and egg chamber maturation. [Date last reviewed: 2018-09-13]

Gene Group (FlyBase)

CYTOCHROME P450 - MITO CLAN -: Cytochrome P450, or CYP genes, constitute one of the largest families of genes, with representatives in virtually all living organisms. P450 enzymes are heme-thiolate proteins best known for their monooxygenase role, catalyzing the transfer of one atom of molecular oxygen to a substrate and reducing the other to water. The MITO clan includes D. melanogaster CYP12, 49, 301, 302, 314 and 315 family genes. Members are localized to mitochondria and at least some are linked to the ecdysteroid metabolism pathway. (Adapted from FBrf0248421.)

Protein Function (UniProtKB)

Required for CNS development; midline glial cells. Involved in the metabolism of insect hormones; responsible for all ecdysone 20-monooxygenase activity during embryonic, larval and adult stages. May be involved in the breakdown of synthetic insecticides.

(UniProt, Q9VUF8)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

shd: shade

Embryonic lethal. No differentiation of cuticle or head skeleton.

Gene Model and Products

Number of Transcripts

3

Number of Unique Polypeptides

2

Please see the JBrowse view of Dmel\shd for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VUF8)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0075676

2730

540

FBtr0304700

1633

433

FBtr0333031

2985

540

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0089324

61.7

540

7.09

FBpp0293243

49.0

433

5.02

FBpp0305245

61.7

540

7.09

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

540 aa isoforms: shd-PB, shd-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\shd using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

shd_1
shd_2

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 12

dorsal epidermis primordium

(Fisher et al., 2012)

embryonic stage 11 -- 16

embryonic epidermis

(Chung et al., 2009)

embryonic stage 13 -- 16

embryonic dorsal epidermis

(Fisher et al., 2012)

third instar larval stage

(Chung et al., 2009)

embryonic/larval midgut

Comment: m6-12

(Chung et al., 2009)

embryonic/larval fat body

(Chung et al., 2009)

embryonic/larval Malpighian tubule

(Chung et al., 2009)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in mitochondrion

inferred from direct assay

(Petryk et al., 2003)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\shd in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\shd

View exonic expression by tissue for Dmel\shd

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003388

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39592/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003388

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003388

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003388

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13478

Flygut - An atlas of the Drosophila adult midgut

FBgn0003388

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\shd

Stages(s) 11-12

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of shd

Transgenic constructs containing regulatory region of shd

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation ( 6 )

Gene disrupted in

(Tearle and Nusslein-Volhard, 1987, Jurgens et al., 1984)

(Ryder, 2004.4.26)

(Giesen et al., 2003, Harbecke and Lengyel, 1995)

(Giesen, 2001.1.24)

Gene not disrupted in

(Giesen et al., 2003, Giesen, 2001.1.24)

(Tearle and Nusslein-Volhard, 1987)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during embryonic stage

lethal - all die during embryonic stage | recessive

shd^unspecified

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

shd^GD8033

viable, with Scer\GAL4^pnr-MD237

shd^GD8033

viable, with Scer\GAL4^twi.2PE, shd¹/shd²

shd^UAS.cPa

viable (with shd¹), with Scer\GAL4^twi.2PE, shd^UAS.cPa

shd²

viable (with shd²), with Scer\GAL4^twi.2PE, shd^UAS.cPa

shd¹

Sterility

Allele

female semi-fertile, with Scer\GAL4^GMR47A04

shd^GD8033

female sterile, with Scer\GAL4^twi.2PE, shd¹/shd²

shd^UAS.cPa

female sterile (with shd¹), with Scer\GAL4^twi.2PE, shd^UAS.cPa

shd²

female sterile (with shd²), with Scer\GAL4^twi.2PE, shd^UAS.cPa

shd¹

Other Phenotypes

Allele

abnormal developmental rate | prepupal stage, with Scer\GAL4^C855a

shd^UAS.cOa

abnormal developmental rate | prepupal stage, with Scer\GAL4^Cg.PA

abnormal developmental rate | prepupal stage, with Scer\GAL4^elav.PLu

shd^UAS.cOa

abnormal developmental rate | prepupal stage, with Scer\GAL4^how-24B

shd^UAS.cOa

abnormal developmental rate | prepupal stage, with Scer\GAL4^Mef2.PR

shd^UAS.cOa

decreased cell number | adult stage, with Scer\GAL4^Tk.PS

shd^HMC06461

some die during embryonic stage

some die during embryonic stage | recessive

shd^unspecified

Phenotype manifest in

Allele

adult anterior midgut, with Scer\GAL4^Tk.PS

shd^HMC06461

adult enteroendocrine cell | decreased number, with Scer\GAL4^Tk.PS

shd^HMC06461

border follicle cell | somatic clone

shd²

cephalopharyngeal skeleton

egg chamber, with Scer\GAL4^twi.2PE, shd¹/shd²

shd^UAS.cPa

egg chamber (with shd¹), with Scer\GAL4^twi.2PE, shd^UAS.cPa

shd²

egg chamber (with shd²), with Scer\GAL4^twi.2PE, shd^UAS.cPa

shd¹

embryo | dorsal closure stage

embryo | embryonic stage 14

shd²

embryonic/first instar larval cuticle

embryonic/larval cuticle

shd³

embryonic/larval foregut

shd^unspecified

embryonic/larval hindgut

shd^unspecified

embryonic/larval midgut

shd^unspecified

embryonic/larval midgut primordium

embryonic/larval tracheal system

shd²

embryonic dorsal epidermis

embryonic epidermis

shd^unspecified

embryonic midgut constriction

shd²

embryonic ventral epidermis

shd^unspecified

shd³

shd^unspecified

larval ventral nerve cord

shd^unspecified

lipid droplet | adult stage, with Scer\GAL4^Tk.PS

shd^HMC06461

midline glial cell | ectopic

shd^unspecified

presumptive embryonic/larval central nervous system

presumptive embryonic/larval peripheral nervous system

shd^unspecified

sensory neuron | embryonic stage

shd^unspecified

symmetrical commissure

shd^unspecified

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (8)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

3 of 15

Yes

No

3 of 15

Yes

No

3 of 15

Yes

No

2 of 15

No

No

2 of 15

No

Yes

2 of 15

No

Yes

2 of 15

No

No

1 of 15

No

No

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

3 of 15

Yes

No

3 of 15

Yes

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

Rattus norvegicus (Norway rat) (9)

3 of 13

Yes

No

3 of 13

Yes

No

2 of 13

No

No

2 of 13

No

Yes

2 of 13

No

Yes

2 of 13

No

No

Rnor\LOC100909462

2 of 13

No

Yes

1 of 13

No

Yes

Rnor\LOC100910462

1 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (10)

3 of 12

Yes

No

3 of 12

Yes

No

3 of 12

Yes

No

Xtro\LOC100145331

3 of 12

Yes

No

2 of 12

No

Yes

2 of 12

No

No

2 of 12

No

Yes

Xtro\LOC100496109

2 of 12

No

Yes

Xtro\LOC100496967

1 of 12

No

No

Xtro\LOC101731070

1 of 12

No

Yes

Danio rerio (Zebrafish) (12)

3 of 15

Yes

No

3 of 15

Yes

No

3 of 15

Yes

No

3 of 15

Yes

No

3 of 15

Yes

No

Drer\si:ch211-113j14.1

3 of 15

Yes

No

Drer\si:dkey-91i10.3

3 of 15

Yes

No

Drer\zgc:136333

3 of 15

Yes

No

2 of 15

No

No

2 of 15

No

Yes

2 of 15

No

Yes

2 of 15

No

No

Caenorhabditis elegans (Nematode, roundworm) (8)

2 of 15

Yes

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Arabidopsis thaliana (thale-cress) (5)

1 of 9

Yes

No

1 of 9

Yes

No

1 of 9

Yes

No

1 of 9

Yes

No

1 of 9

Yes

No

Saccharomyces cerevisiae (Brewer's yeast) (1)

1 of 15

Yes

No

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (19)

Gene Symbol

Score

Source

Alignment

4 of 10

4 of 10

4 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

CYP27B1; cytochrome P450 family 27 subfamily B member 1

model of vitamin D-dependent rickets type 1A

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CYP24A1; cytochrome P450 family 24 subfamily A member 1

3 of 15

HYPERCALCEMIA, INFANTILE, 1; HCINF1

CYP27B1; cytochrome P450 family 27 subfamily B member 1

3 of 15

VITAMIN D HYDROXYLATION-DEFICIENT RICKETS, TYPE 1A; VDDR1A

vitamin D-dependent rickets type 1A

CYP27C1; cytochrome P450 family 27 subfamily C member 1

3 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0003388

MIST (protein-protein) - An integrated Molecular Interaction Database

shd

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Insect hormone biosynthesis

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3L

Recombination map

3-42

Cytogenetic map

Sequence location

3L:14,614,458..14,622,205 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

70E4-70E4

Limits computationally determined from genome sequence between P{PZ}Mpcp⁰⁰⁵⁶⁴ and P{PZ}l(3)rO220^rO220

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

70E4-70E5

(Dunkov, 2000.6.29)

Experimentally Determined Recombination Data

Location

3-42

(FlyBase, 2016)

3-36.3

(Comeron et al., 2012)

3-41

(Tearle and Nusslein-Volhard, 1987)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Aberrations including deletions of this gene Aberrations including duplications of this gene

34507

shd¹ st¹ e¹/TM3, Sb¹

67356

y¹ sc^* v¹ sev²¹; P{TRiP.HMC06461}attP40

4219

ru¹ h¹ shd² Diap1¹ st¹ cu¹ sr¹ e^s ca¹/TM3, Sb¹ Ser¹

107639

ru¹ h¹ shd² Diap1¹ st¹ cu¹ sr¹ e^s ca¹/TM3, Sb¹

v108911

P{KK106072}VIE-260B

v17203

w¹¹¹⁸; P{GD8033}v17203

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (10)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

AT11204

Other clones

DPiM_AT11204

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003388

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003388

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39592

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: shd Cyp314a1

(Petryk, 2002.2.15)

Additional comments

Other Comments

Four EMS induced alleles have been identified in a screen for mutations affecting commissure formation in the CNS of the embryo.

(Hummel et al., 1999)

shd mutants display no differentiation of the cuticle or head skeleton.

(Jurgens et al., 1984)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 44 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39592

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VUF8

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VUF8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9PI59

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VUF8

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003388

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003388

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39592/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003388

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

shd_1
shd_2

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003388

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003388

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13478

Flygut - An atlas of the Drosophila adult midgut

FBgn0003388

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39592

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC011231

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG13478

MARRVEL_MODEL - MARRVEL (model organism gene)

39592

modMine - A data warehouse for the modENCODE project

FBgn0003388

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0003388

DRSC - Results frm RNAi screens

FBgn0003388

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003388

FlyMine - An integrated database for Drosophila genomics

FBgn0003388

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Insect hormone biosynthesis

MIST (protein-protein) - An integrated Molecular Interaction Database

shd

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

314a1

(Nelson, 2001.2.5)

CG13478

(Hosono et al., 2015, Keleman et al., 2009.8.5, Perkins et al., 2009)

CYP314A1

(Pan et al., 2020, Chen and Li, 2007, Rewitz et al., 2007)

Cyp314a1

(Scanlan et al., 2020, Good et al., 2014, Niwa et al., 2011, Rewitz et al., 2007, Ranson et al., 2002, Tijet et al., 2001)

Shade

(Sood et al., 2021)

cyp314a1

(Gangishetti et al., 2009)

shade

(Meschi and Delanoue, 2021, Jacobs et al., 2020, Gangishetti et al., 2009)

Name Synonyms

Shade

(Kannangara et al., 2021, Niwa and Niwa, 2016, Pahi et al., 2015, Heimiller et al., 2014)

cytochrome P450

(Chung et al., 2009)

ecdysone 20-monooxygenase

(Rewitz et al., 2007)

shade

(Christensen et al., 2020, Buhler et al., 2018, Akagi et al., 2016, Saadin and Starz-Gaiano, 2016, Wang et al., 2016, Wieschaus and Nüsslein-Volhard, 2016, Borsos et al., 2015, Domanitskaya et al., 2014, Chavoshi et al., 2010, Pankotai et al., 2010, Xiang et al., 2010, Huang et al., 2008, Rewitz et al., 2007, Hummel et al., 1999)

Secondary FlyBase IDs

FBgn0036432

Datasets (6)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (6)

Result

Result Type

Title

scRNAseq_2020_Cattenoz_NI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from non-infested third instar larvae

scRNAseq_2020_Cattenoz_WI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from wasp-infested third instar larvae

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (104)