• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\shf

General Information
Symbol	Dmel\shf	Species	D. melanogaster
Name	shifted	Annotation symbol	CG3135
Feature type	protein_coding_gene	FlyBase ID	FBgn0003390
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	DmWif
Genomic Location
Chromosome (arm)	X	Recombination map	1-17.9
Cytogenetic map	6C13-6D1	Sequence location	X:6,665,477..6,673,692 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene shifted is referred to in FlyBase by the symbol Dmel\shf (CG3135, FBgn0003390). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: smoothened signaling pathway; regulation of smoothened signaling pathway; protein stabilization; Wnt receptor signaling pathway. 20 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; adult segment; adult mesothoracic segment; external compound sense organ; organ system; integumentary plate; compound cell cluster organ; sensillum; wing cell; peripheral nervous system. It has one annotated transcript and one annotated polypeptide. Protein features are: EGF-like region, conserved site; Epidermal growth factor-like; Epidermal growth factor-like, type 3; WIF domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult crop. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult hindgut, adult heart, adult fat body, adult spermathecae, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1635403_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of shf. Gene sequence location is X:6665477..6673692.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	*shf: shifted Vein L3 fails to reach wing margin and is shifted toward L4. Anterior crossvein usually lacking. Wings divergent. Postscutellar bristles small and erect. Body small. Viability 60% wild type. Female often sterile. RK2. shf2 Veins closer together than in wild type. L3 and L4 tend to fuse near anterior crossvein; anterior crossvein shortened, knotted, or absent. Phenotypic effect visible in prepupal wing bud, the two longitudinal veins diverging at a smaller-than-normal angle [Waddington, 1940, J. Genet. 41: 75-139 (fig.)]. Eyes sometimes slightly rough. Scutellar bristles often absent. Scutellum short. Wings narrow and often warped downward. Fertility and viability good. RK2. *shf3 shf3: shifted-3 From Bridges and Brehme, 1944, Carnegie Inst. Washington Publ. No. 552: 173. Like shf2 but more extreme. Viability about 70% wild type. Frequently infertile. RK2. shfov: shifted-oval Eyes rough and narrow. First basal wing cell absent because L3 and L4 veins close. Wings narrow and pointed. Viability and fertility low. RK2.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	Sequence features HFA18283 BKN29358
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 6C13-6D1   Limits computationally determined from genome sequence between P{EP}Ctr1AEP1364&P{EP}EP1445 and P{EP}C3GEP1613
Experimentally Determined Cytological Location
Cytogenetic map Notes References 6A3-6F11   Determined by deletion analysis (details unspecified).   (Demerec et al., 1942)
Experimentally Determined Recombination Data
Location	1-17.9   1-17 (Bateman, 1950)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\shf for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) shf-RA FBtr0070978  NM_132121   1803   456
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein shf-PA   FBpp0070939   50.7   456   8.85     NP_572349   AAF46198
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Epidermal growth factor-like, type 3 (IPR000742) WIF domain (IPR003306) Epidermal growth factor-like (IPR006210) EGF-like region, conserved site (IPR013032)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014298 AAF46198   AI063402   AI387857   AI514002   AW941041   AY051622 AAK93046   BI638219   CO263187   CO314200   CO322218   CO341736   CZ482338   EC059255   EC070740   EC219465   EC219466   EC223502   EC225780   GH943667   GH943889   GH946500   GH948722   GH949334   GH950094   GH950654   GH954884   GH961324   GH973671   GH978428   GH981794   GH983573   GH984443   GH984825
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9W3W5
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0003390    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0003390 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed at stages throughout the pupal period. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0003390 expression level Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    49 embryo 04-06hr    83 embryo 06-08hr    285 embryo 08-10hr    229 embryo 10-12hr    192 embryo 12-14hr    124 embryo 14-16hr    208 embryo 16-18hr    403 embryo 18-20hr    534 embryo 20-22hr    739 embryo 22-24hr    465 larva L1    258 larva L2    266 larva L3 12hr old    284 larva L3 puffstage 1-2    620 larva L3 puffstage 3-6    934 larva L3 puffstage 7-9    1245 white prepupae new    1282 white prepupae 12hr    2883 white prepupae 24hr    3333 pupae 2d postWPP    1509 pupae 3d postWPP    386 pupae 4d postWPP    1399 adult male 01day    1355 adult male 05day    1162 adult male 30day    850 adult female 01day    1073 adult female 05day    234 adult female 30day    252 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    49 embryo 04-06hr    83 embryo 06-08hr  (285) embryo 08-10hr  (229) embryo 10-12hr  (192) embryo 12-14hr  (124) embryo 14-16hr  (208) embryo 16-18hr  (403) embryo 18-20hr  (534) embryo 20-22hr  (739) embryo 22-24hr  (465) larva L1  (258) larva L2  (266) larva L3 12hr old  (284) larva L3 puffstage 1-2  (620) larva L3 puffstage 3-6  (934) larva L3 puffstage 7-9  (1245) white prepupae new  (1282) white prepupae 12hr  (2883) white prepupae 24hr  (3333) pupae 2d postWPP  (1509) pupae 3d postWPP  (386) pupae 4d postWPP  (1399) adult male 01day  (1355) adult male 05day  (1162) adult male 30day  (850) adult female 01day  (1073) adult female 05day  (234) adult female 30day  (252) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    49 embryo 04-06hr    83 embryo 06-08hr    285 embryo 08-10hr    229 embryo 10-12hr    192 embryo 12-14hr    124 embryo 14-16hr    208 embryo 16-18hr    403 embryo 18-20hr    534 embryo 20-22hr    739 embryo 22-24hr    465 larva L1    258 larva L2    266 larva L3 12hr old    284 larva L3 puffstage 1-2    620 larva L3 puffstage 3-6    934 larva L3 puffstage 7-9    1245 white prepupae new    1282 white prepupae 12hr  (2883) white prepupae 24hr  (3333) pupae 2d postWPP  1509 pupae 3d postWPP    386 pupae 4d postWPP    1399 adult male 01day    1355 adult male 05day    1162 adult male 30day    850 adult female 01day    1073 adult female 05day    234 adult female 30day    252 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    49 embryo 04-06hr    83 embryo 06-08hr    285 embryo 08-10hr    229 embryo 10-12hr    192 embryo 12-14hr    124 embryo 14-16hr    208 embryo 16-18hr    403 embryo 18-20hr    534 embryo 20-22hr    739 embryo 22-24hr    465 larva L1    258 larva L2    266 larva L3 12hr old    284 larva L3 puffstage 1-2    620 larva L3 puffstage 3-6    934 larva L3 puffstage 7-9    1245 white prepupae new    1282 white prepupae 12hr    2883 white prepupae 24hr    3333 pupae 2d postWPP    1509 pupae 3d postWPP    386 pupae 4d postWPP    1399 adult male 01day    1355 adult male 05day    1162 adult male 30day    850 adult female 01day    1073 adult female 05day    234 adult female 30day    252 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    49 embryo 04-06hr    83 embryo 06-08hr    285 embryo 08-10hr    229 embryo 10-12hr    192 embryo 12-14hr    124 embryo 14-16hr    208 embryo 16-18hr    403 embryo 18-20hr    534 embryo 20-22hr    739 embryo 22-24hr    465 larva L1    258 larva L2    266 larva L3 12hr old    284 larva L3 puffstage 1-2    620 larva L3 puffstage 3-6    934 larva L3 puffstage 7-9    1245 white prepupae new    1282 white prepupae 12hr    2883 white prepupae 24hr    3333 pupae 2d postWPP    1509 pupae 3d postWPP    386 pupae 4d postWPP    1399 adult male 01day    1355 adult male 05day    1162 adult male 30day    850 adult female 01day    1073 adult female 05day    234 adult female 30day    252 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0003390 expression level Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    49 embryo 04-06hr    83 embryo 06-08hr    285 embryo 08-10hr    229 embryo 10-12hr    192 embryo 12-14hr    124 embryo 14-16hr    208 embryo 16-18hr    403 embryo 18-20hr    534 embryo 20-22hr    739 embryo 22-24hr    465 larva L1    258 larva L2    266 larva L3 12hr old    284 larva L3 puffstage 1-2    620 larva L3 puffstage 3-6    934 larva L3 puffstage 7-9    1245 white prepupae new    1282 white prepupae 12hr    2883 white prepupae 24hr    3333 pupae 2d postWPP    1509 pupae 3d postWPP    386 pupae 4d postWPP    1399 adult male 01day    1355 adult male 05day    1162 adult male 30day    850 adult female 01day    1073 adult female 05day    234 adult female 30day    252 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    49 embryo 04-06hr    83 embryo 06-08hr  (285) embryo 08-10hr  (229) embryo 10-12hr  (192) embryo 12-14hr  124 embryo 14-16hr  (208) embryo 16-18hr  (403) embryo 18-20hr  (534) embryo 20-22hr  (739) embryo 22-24hr  (465) larva L1  (258) larva L2  (266) larva L3 12hr old  (284) larva L3 puffstage 1-2  (620) larva L3 puffstage 3-6  (934) larva L3 puffstage 7-9  (1245) white prepupae new  (1282) white prepupae 12hr  (2883) white prepupae 24hr  (3333) pupae 2d postWPP  (1509) pupae 3d postWPP  (386) pupae 4d postWPP  (1399) adult male 01day  (1355) adult male 05day  (1162) adult male 30day  (850) adult female 01day  (1073) adult female 05day  (234) adult female 30day  (252) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    49 embryo 04-06hr    83 embryo 06-08hr    285 embryo 08-10hr    229 embryo 10-12hr    192 embryo 12-14hr    124 embryo 14-16hr    208 embryo 16-18hr    403 embryo 18-20hr    534 embryo 20-22hr    739 embryo 22-24hr    465 larva L1    258 larva L2    266 larva L3 12hr old    284 larva L3 puffstage 1-2    620 larva L3 puffstage 3-6    934 larva L3 puffstage 7-9    1245 white prepupae new    1282 white prepupae 12hr  (2883) white prepupae 24hr  (3333) pupae 2d postWPP  1509 pupae 3d postWPP    386 pupae 4d postWPP    1399 adult male 01day    1355 adult male 05day    1162 adult male 30day    850 adult female 01day    1073 adult female 05day    234 adult female 30day    252 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    49 embryo 04-06hr    83 embryo 06-08hr    285 embryo 08-10hr    229 embryo 10-12hr    192 embryo 12-14hr    124 embryo 14-16hr    208 embryo 16-18hr    403 embryo 18-20hr    534 embryo 20-22hr    739 embryo 22-24hr    465 larva L1    258 larva L2    266 larva L3 12hr old    284 larva L3 puffstage 1-2    620 larva L3 puffstage 3-6    934 larva L3 puffstage 7-9    1245 white prepupae new    1282 white prepupae 12hr    2883 white prepupae 24hr    3333 pupae 2d postWPP    1509 pupae 3d postWPP    386 pupae 4d postWPP    1399 adult male 01day    1355 adult male 05day    1162 adult male 30day    850 adult female 01day    1073 adult female 05day    234 adult female 30day    252 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    49 embryo 04-06hr    83 embryo 06-08hr    285 embryo 08-10hr    229 embryo 10-12hr    192 embryo 12-14hr    124 embryo 14-16hr    208 embryo 16-18hr    403 embryo 18-20hr    534 embryo 20-22hr    739 embryo 22-24hr    465 larva L1    258 larva L2    266 larva L3 12hr old    284 larva L3 puffstage 1-2    620 larva L3 puffstage 3-6    934 larva L3 puffstage 7-9    1245 white prepupae new    1282 white prepupae 12hr    2883 white prepupae 24hr    3333 pupae 2d postWPP    1509 pupae 3d postWPP    386 pupae 4d postWPP    1399 adult male 01day    1355 adult male 05day    1162 adult male 30day    850 adult female 01day    1073 adult female 05day    234 adult female 30day    252 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0003390    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0003390 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult crop. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult hindgut, adult heart, adult fat body, adult spermathecae, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0003390 expression level Tissue   Expression Level Larval Central Nervous System    60.175 Larval Midgut no informative data Larval Hindgut    47.7 Larval Malpighian Tubules no informative data Larval Fat Body    39.4 Larval Salivary Gland no informative data Larval Trachea    39.875 Larval Carcass    73.45 Adult Head    154.2 Adult Eye    96.05 Adult Brain    19 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    1577.4 Adult Midgut    98.6 Adult Hindgut    270.1 Adult Malpighian Tubules no informative data Adult Fat Body    312.7 Adult Salivary Gland no informative data Adult Heart    249.3 Adult VirginFemale Spermatheca    297.2 Adult InseminatedFemale Spermatheca    284.1 Adult Ovary    5.1 Adult Testis    33.4 Adult Male Accessory Gland    5.7 Adult Carcass    165.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    60.175 Larval Midgut no informative data Larval Hindgut    47.7 Larval Malpighian Tubules no informative data Larval Fat Body    39.4 Larval Salivary Gland no informative data Larval Trachea    39.875 Larval Carcass    73.45 Adult Head    154.2 Adult Eye    96.05 Adult Brain    19 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop  (1577.4) Adult Midgut    98.6 Adult Hindgut    270.1 Adult Malpighian Tubules no informative data Adult Fat Body    312.7 Adult Salivary Gland no informative data Adult Heart    249.3 Adult VirginFemale Spermatheca    297.2 Adult InseminatedFemale Spermatheca    284.1 Adult Ovary    5.1 Adult Testis    33.4 Adult Male Accessory Gland    5.7 Adult Carcass    165.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    60.175 Larval Midgut no informative data Larval Hindgut    47.7 Larval Malpighian Tubules no informative data Larval Fat Body    39.4 Larval Salivary Gland no informative data Larval Trachea    39.875 Larval Carcass    73.45 Adult Head    154.2 Adult Eye    96.05 Adult Brain    19 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop  (1577.4) Adult Midgut    98.6 Adult Hindgut    270.1 Adult Malpighian Tubules no informative data Adult Fat Body    312.7 Adult Salivary Gland no informative data Adult Heart    249.3 Adult VirginFemale Spermatheca    297.2 Adult InseminatedFemale Spermatheca    284.1 Adult Ovary    5.1 Adult Testis    33.4 Adult Male Accessory Gland    5.7 Adult Carcass    165.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    60.175 Larval Midgut no informative data Larval Hindgut    47.7 Larval Malpighian Tubules no informative data Larval Fat Body    39.4 Larval Salivary Gland no informative data Larval Trachea    39.875 Larval Carcass    73.45 Adult Head    154.2 Adult Eye    96.05 Adult Brain    19 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    1577.4 Adult Midgut    98.6 Adult Hindgut    270.1 Adult Malpighian Tubules no informative data Adult Fat Body    312.7 Adult Salivary Gland no informative data Adult Heart    249.3 Adult VirginFemale Spermatheca    297.2 Adult InseminatedFemale Spermatheca    284.1 Adult Ovary    5.1 Adult Testis    33.4 Adult Male Accessory Gland    5.7 Adult Carcass    165.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0003390 expression level Tissue   Expression Level Larval Central Nervous System    60.175 Larval Midgut no informative data Larval Hindgut    47.7 Larval Malpighian Tubules no informative data Larval Fat Body    39.4 Larval Salivary Gland no informative data Larval Trachea    39.875 Larval Carcass    73.45 Adult Head    154.2 Adult Eye    96.05 Adult Brain    19 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    1577.4 Adult Midgut    98.6 Adult Hindgut    270.1 Adult Malpighian Tubules no informative data Adult Fat Body    312.7 Adult Salivary Gland no informative data Adult Heart    249.3 Adult VirginFemale Spermatheca    297.2 Adult InseminatedFemale Spermatheca    284.1 Adult Ovary    5.1 Adult Testis    33.4 Adult Male Accessory Gland    5.7 Adult Carcass    165.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    60.175 Larval Midgut no informative data Larval Hindgut    47.7 Larval Malpighian Tubules no informative data Larval Fat Body    39.4 Larval Salivary Gland no informative data Larval Trachea    39.875 Larval Carcass    73.45 Adult Head    154.2 Adult Eye    96.05 Adult Brain    19 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop  (1577.4) Adult Midgut    98.6 Adult Hindgut    270.1 Adult Malpighian Tubules no informative data Adult Fat Body    312.7 Adult Salivary Gland no informative data Adult Heart    249.3 Adult VirginFemale Spermatheca    297.2 Adult InseminatedFemale Spermatheca    284.1 Adult Ovary    5.1 Adult Testis    33.4 Adult Male Accessory Gland    5.7 Adult Carcass    165.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    60.175 Larval Midgut no informative data Larval Hindgut    47.7 Larval Malpighian Tubules no informative data Larval Fat Body    39.4 Larval Salivary Gland no informative data Larval Trachea    39.875 Larval Carcass    73.45 Adult Head    154.2 Adult Eye    96.05 Adult Brain    19 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop  1577.4 Adult Midgut    98.6 Adult Hindgut    270.1 Adult Malpighian Tubules no informative data Adult Fat Body    312.7 Adult Salivary Gland no informative data Adult Heart    249.3 Adult VirginFemale Spermatheca    297.2 Adult InseminatedFemale Spermatheca    284.1 Adult Ovary    5.1 Adult Testis    33.4 Adult Male Accessory Gland    5.7 Adult Carcass    165.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    60.175 Larval Midgut no informative data Larval Hindgut    47.7 Larval Malpighian Tubules no informative data Larval Fat Body    39.4 Larval Salivary Gland no informative data Larval Trachea    39.875 Larval Carcass    73.45 Adult Head    154.2 Adult Eye    96.05 Adult Brain    19 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    1577.4 Adult Midgut    98.6 Adult Hindgut    270.1 Adult Malpighian Tubules no informative data Adult Fat Body    312.7 Adult Salivary Gland no informative data Adult Heart    249.3 Adult VirginFemale Spermatheca    297.2 Adult InseminatedFemale Spermatheca    284.1 Adult Ovary    5.1 Adult Testis    33.4 Adult Male Accessory Gland    5.7 Adult Carcass    165.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut     Larval Malpighian Tubules no informative data Larval Fat Body     Larval Salivary Gland no informative data Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion no informative data Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules no informative data Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    154.2   NA  Eye    96.05   NA  Brain    19   60.175  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    1577.4   no informative data  Midgut    98.6   47.7  Hindgut    270.1   no informative data  Malpighian Tubules    no informative data   39.4  Fat Body    312.7   no informative data  Salivary Gland    no informative data   NA  Heart    249.3   39.875  Trachea    NA   NA  VirginFemale Spermatheca    297.2   NA  InseminatedFemale Spermatheca    284.1   NA  Ovary    5.1   NA  Testis    33.4   NA  Male Accessory Gland    5.7   73.45  Carcass    165.1 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003390 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3135-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for shf Stages(s) 1-3 Stages(s) 11-12
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage shf919 semi-lethal | recessive shfEY03173 semi-viable shf1 shf2 viable shf2 shfov shfx33 viable, with Scer\GAL4pnr-MD237 shfGD6297 viable | reduced shf3 Sterility Allele female sterile shf1 fertile shf2 fertile | poor shfov semi-sterile shf2 sterile shf3 Other Phenotypes Allele small body shf1 shf2 visible shfov Phenotype manifest in Allele 1st posterior cell shf2 shfEY03173 shfP1 shfx33 anterior crossvein shf1 shf2 denticle belt shf919 eye shf2 shfov shfx33 metathoracic leg, with Scer\GAL4ptc-559.1 shfEY03173 ommatidium shfov posterior scutellar bristle shf1 shf2 shfov postvertical bristle shfov scutellar bristle shf2 shfx33 scutellum shf2 shfx33 wing shf1 shf2 shf919 shfEY03173 shfP1 wing basal cell 1 shfov wing vein shf2 wing vein L3 shf1 shf2 shf919 shfov shfx33 wing vein L4 shf2 shf919 shfov
Classical Alleles ( 10 )
For All Classical Alleles Show
Allele of shf Class Mutagen Stocks Known lesion shf2 X ray 1 Yes shfe04584 piggyBac transposase 1 -- shfEY03173 P-element activity 1 -- shf1 0 -- shf3 spontaneous 0 -- shf919 0 Yes shfov 32P 0 -- shfP1 Delta2-3 0 Yes shfx13 P-element activity 0 Yes shfx33 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of shf Class Mutagen Stocks Known lesion shfGD6297 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes shfdsRNA.cYa in vitro construct - RNAi 0 Yes shfdsRNA.Scer\UAS.220 in vitro construct - RNAi 0 Yes shfdsRNA.Scer\UAS.738 in vitro construct - RNAi 0 Yes shfScer\UAS.cGa in vitro construct - regulatory fusion 0 Yes shfScer\UAS.cGb in vitro construct - regulatory fusion 0 Yes shfScer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes shfScer\UAS.T:Avic\GFP-YFP in vitro construct - coding region fusion 0 Yes shfδEGF.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes shfδWIF.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)2.5 (Gorfinkiel et al., 2005) Df(1)BSC276 (Christensen and Cook, 2007.5.8) Df(1)BSC285 (Christensen and Cook, 2007.5.8) Df(1)BSC286 (Christensen and Cook, 2007.5.8) Df(1)BSC297 (Christensen and Cook, 2007.5.8) Df(1)BSC351 (Christensen and Cook, 2007.10.29) Df(1)ct268-37
Not duplicated in	Dp(1;f)q2
Duplicated in	Dp(1;3)ct268-37 (Demerec, 1940) Dp(1;3)sn13a1 (Craymer and Roy, 1980)
Not disrupted in	Df(1)ct-J4 (Craymer and Roy, 1980) Df(1)HA32 (Craymer and Roy, 1980)
Partially disrupted in	Df(1)x13 (Glise et al., 2005, Glise et al., 2005)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD6297} NA P{UAS-shf.G} NA P{UAS-shf.YFP} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}shfEY03173 NA miscellaneous insertions P{CaSpeR}shfP1 NA PBac{RB}COQ7e04529 NA PBac{RB}shfe04584 NA PBac{WH}COQ7f04093 NA
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with hh (assigned by UniProtKB) (Gorfinkiel et al., 2005)
Biological Process
CV term Evidence References
protein stabilization inferred from mutant phenotype (assigned by UniProtKB) (Gorfinkiel et al., 2005) regulation of smoothened signaling pathway inferred from mutant phenotype (Glise et al., 2005) smoothened signaling pathway inferred from genetic interaction with ptc AND inferred from mutant phenotype (assigned by UniProtKB) (Gorfinkiel et al., 2005) inferred from mutant phenotype (assigned by UniProtKB) (Glise et al., 2005) Wnt receptor signaling pathway inferred from mutant phenotype (assigned by UniProtKB) (Gorfinkiel et al., 2005, Glise et al., 2005)
Cellular Component
CV term Evidence References
extracellular region inferred from direct assay (assigned by UniProtKB) (Glise et al., 2005) proteinaceous extracellular matrix inferred from direct assay (assigned by UniProtKB) (Gorfinkiel et al., 2005)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function
CV term Evidence References
protein tyrosine kinase activity inferred from electronic annotation with InterPro:IPR003306 (FlyBase Curators et al., 2004-)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions shf allele Gene References unspecified rho (Sturtevant and Bier, 1995)
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0003390 DroID - A comprehensive database of gene and protein interactions. • FBgn0003390 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31617
Orthologs
Genome-wide drosophilid orthologs	Dana\GF20838 Dere\GG17656 Dgri\GH24528 Dmoj\GI21608 Dper\GL26878 Dpse\GA16199 Dsec\GM12553 Dsim\GD16182 Dvir\GJ16546 Dwil\GK25838 Dyak\GE16446
Curated drosophilid orthologs	Dpse\GA16199
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL011386-PB Aedes aegypti (mosquito) AGAP000117-PA Anopheles gambiae (mosquito) XP_625183.1 Apis mellifera (honey bee) BGIBMGA005774-PA Bombyx mori (silk worm) ENSBTAP00000019636 Bos taurus (bovine) CN01821 Caenorhabditis brenneri (small nematode) CE35359 Caenorhabditis elegans (worm) ENSCAFP00000000520 Canis familiaris (dog) CPIJ003761 Culex pipens (mosquito) ENSCPOP00000001612 Cavia porcellus (guinea pig) 165770 Capitella spl (polychaete worm) 116992 Daphnia pulex (daphnia) ENSDARP00000019818 Danio rerio (zebra fish) ENSECAP00000022461 Equus caballus (horse) ENSGALP00000016025 Gallus gallus (chicken) 88449 Hypsipyla robusta (cedar shoot borer) ENSP00000286574 Homo sapiens (human) ENSMMUP00000022198 Macaca mulatta (rhesus monkey) ENSMUSP00000020439 Mus musculus (house mouse) XP_001606619.1 Nasonia vitripennis (wasp) ENSOANP00000009416 Ornithorynchus anatinus (platypus) PHUM010144-PA Pediculus humanus (human body louse) ENSPPYP00000005391 Pongo pygmaeus (orangutan) ENSPTRP00000008803 Pan troglodytes (chimpanzee) ENSRNOP00000006426 Rattus norvegicus (norway rat) XP_787507.2 Strongylocentrotus purpuratus (purple sea urchin) XP_967866.1 Tribolium castaneum (red flour beetle) ENSXETP00000030283 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB14648 NV12774 LH23395 ISCW021962 FBgn0233971 FBgn0227797 FBgn0203730 FBgn0187811 FBgn0167490 JGI_V11_116992 FBgn0076215 FBgn0164459 FBgn0144338 FBgn0131984 FBgn0109878 FBgn0097844 CPIJ003761 BGIBMGA005774 ACYPI49270 ACYPI28150 GB10372 AGAP000117 AAEL011386 PHUM477220 GLEAN_01979
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	112 shf2 15899 y1 w67c23 P{EPgy2}shfEY03173/FM7c
Harvard	- PBac{RB}shfe04584
VDRC	v14803 w1118; P{GD6297}v14803/CyO
Genomic Clones ( 1 )
	BACR48O24 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 28 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH27042
Other clones	DPiM_GH27042
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH03190 GH18532 SD20453
Other clones	62348 108601 CK01.102C.B12 CK01.104D.E7 EK087720 EK187561 EK235414 EP13047 RP004107363 RP004170888 RP004176719 RP004186586 RP004194783 RP004198635 RP004207700 RP004219695 RP004227640 RP004246178 RP004249160 RP004260422 RP004377921 RP004536482 RP004554507
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003390 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31617
Antibody Information
	polyclonal (Glise et al., 2005)
Other Information
Discoverer
	Bridges, Jan. 1913.
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: shf CG3135 (Guerrero, 2004.2.25)
Additional comments
Other Comments
	Mutants display hyperplastic phenotype, with tissue overgrowth in mitotic recombination clones. (Mechler, 1994) Mutations in shf cause extra longitudinal or transverse veins with plexated vein phenotypes. kn, fu and shf act synergistically against Px, px, net, dsr and emc. (Diaz-Benjumea and Garcia-Bellido, 1990)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_132121 RefSeq (Proteins) • NP_572349 DDBJ / EMBL / GenBank • AAF46198 AAK93046 AI514002 AW941041 AY051622 CZ482338 Entrez Gene - A searchable database of RefSeq genes. • 31617 UniProtKB/TrEMBL • Q9W3W5
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Epidermal growth factor-like, type 3 (IPR000742) WIF domain (IPR003306) Epidermal growth factor-like (IPR006210) EGF-like region, conserved site (IPR013032)
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0003390 DroID - A comprehensive database of gene and protein interactions. • FBgn0003390 DRSC - Results from RNAi screens. • FBgn0003390 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003390 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3135-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003390 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31617 InParanoid A subset of ortholog calls from InParanoid. • AAEL011386-PB Aedes aegypti (mosquito) AGAP000117-PA Anopheles gambiae (mosquito) XP_625183.1 Apis mellifera (honey bee) BGIBMGA005774-PA Bombyx mori (silk worm) ENSBTAP00000019636 Bos taurus (bovine) CN01821 Caenorhabditis brenneri (small nematode) CE35359 Caenorhabditis elegans (worm) ENSCAFP00000000520 Canis familiaris (dog) CPIJ003761 Culex pipens (mosquito) ENSCPOP00000001612 Cavia porcellus (guinea pig) 165770 Capitella spl (polychaete worm) 116992 Daphnia pulex (daphnia) ENSDARP00000019818 Danio rerio (zebra fish) ENSECAP00000022461 Equus caballus (horse) ENSGALP00000016025 Gallus gallus (chicken) 88449 Hypsipyla robusta (cedar shoot borer) ENSP00000286574 Homo sapiens (human) ENSMMUP00000022198 Macaca mulatta (rhesus monkey) ENSMUSP00000020439 Mus musculus (house mouse) XP_001606619.1 Nasonia vitripennis (wasp) ENSOANP00000009416 Ornithorynchus anatinus (platypus) PHUM010144-PA Pediculus humanus (human body louse) ENSPPYP00000005391 Pongo pygmaeus (orangutan) ENSPTRP00000008803 Pan troglodytes (chimpanzee) ENSRNOP00000006426 Rattus norvegicus (norway rat) XP_787507.2 Strongylocentrotus purpuratus (purple sea urchin) XP_967866.1 Tribolium castaneum (red flour beetle) ENSXETP00000030283 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /segment/shifted1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31617 modMine - Data generated by the modENCODE project. • FBgn0003390 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB14648 NV12774 LH23395 ISCW021962 FBgn0233971 FBgn0227797 FBgn0203730 FBgn0187811 FBgn0167490 JGI_V11_116992 FBgn0076215 FBgn0164459 FBgn0144338 FBgn0131984 FBgn0109878 FBgn0097844 CPIJ003761 BGIBMGA005774 ACYPI49270 ACYPI28150 GB10372 AGAP000117 AAEL011386 PHUM477220 GLEAN_01979 REDfly - A database of transcriptional regulatory elements. • FBgn0003390
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	CG3134 (Gorfinkiel et al., 2005) CG3135 (Fujikawa et al., 2009, Glise et al., 2005) CT10514 (Hynes and Zhao, 2000) DmWif (Glise et al., 2003) DmWIF (Gorfinkiel et al., 2005) D-Wif (Gorfinkiel et al., 2003) shf (Dionne et al., 2006, Christensen and Cook, 2007.5.8, Yao et al., 2006, Christensen and Cook, 2007.5.8, Christensen and Cook, 2007.5.8, Christensen and Cook, 2007.5.8, Christensen and Cook, 2007.10.29, Aerts et al., 2007, Torroja et al., 2005, Fujikawa et al., 2009, Zeitlinger et al., 2007, Glise et al., 2005, Parrish et al., 2009, Ostrin et al., 2006, Zhai et al., 2010) Shf (Gorfinkiel et al., 2005, Callejo et al., 2007, Wilson and Chuang, 2006) shf-ov (Bateman, 1950)
Name Synonym	shifted (Briscoe and Therond, 2005, Kurusu et al., 2008, Wendler et al., 2006, Torroja et al., 2005, Ostrin et al., 2006, Zhai et al., 2010) Shifted (Callejo et al., 2007, Wilson and Chuang, 2006) WNT inhibitory factor (Banyai and Patthy, 2004)
Secondary FlyBase IDs
	FBgn0029901
References ( 59 )
Generate a list of	All references relating to this gene ( 59 )
List References by type	Research paper  ( 21 ) Supplementary material  ( 3 ) Review  ( 7 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 11 ) FlyBase analysis  ( 1 ) Meeting report  ( 1 ) Book  ( 1 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 1 )
	Zhai et al., 2010, Europ. J. Cell Biol. 89(2-3): 273--278 Cellular analysis of newly identified Hox downstream genes in Drosophila. [FBrf0209935] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...