Symbol Dmel\ smoSpecies D. melanogaster Name smoothened Annotation symbol CG11561 Feature type protein_coding_gene FlyBase ID FBgn0003444 Gene Model Status Current Stock availability 10 publicly
available Also Known As dSmo Chromosome (arm) 2L Recombination map 2-0.4 Cytogenetic map 21B7-21B7 Sequence location 2L:277,587..282,167 [+]
Genomic Maps
Automatically generated summary
The gene
smoothened is referred to in FlyBase by the symbol
Dmel\smo (CG11561, FBgn0003444). It is a protein_coding_gene from
Drosophila melanogaster . There is experimental evidence that it has the
molecular function : protein binding. There is experimental evidence that it is involved in the
biological process : blastoderm segmentation; smoothened signaling pathway; eye-antennal disc morphogenesis; ovarian follicle cell development; somatic stem cell maintenance; regulation of mitotic cell cycle; negative regulation of S phase of mitotic cell cycle; eye morphogenesis; Bolwig's organ morphogenesis.
252 alleles are reported . The
phenotypes of these alleles are annotated with: organ system; adult segment; organ system subdivision; larval abdominal segment; thoracic segment; imaginal precursor; portion of tissue; non-connected developing system; tracheal system; abdominal 3 ventral denticle belt; heart primordium; abdominal 5 ventral denticle belt; epithelial furrow; external compound sense organ. It has
one annotated transcript and
one annotated polypeptide .
Protein features are: Frizzled domain; Frizzled protein; Frizzled/secreted frizzled-related protein; GPCR, family 2-like; Smoothened. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult crop, larval/adult hindgut, larval Malpighian tubules, adult heart, adult fat body, larval trachea, adult female reproductive system, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1634442_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of smo. Gene sequence location is
2L:277587..282167 .
User Contributed Data External Summaries
Gene/Allele symbols may differ
from current usage smo: smoothened
Embryonic lethal. All denticles in abdominal segments point posteriorly. At 18 naked cuticle deleted and denticle belts of adjacent segments fused and locally arranged as
mirror-image duplications.
Description
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It currently only tracks new links between this FlyBase report and other
FlyBase data classes (e.g. genes, references, stocks) or controlled
vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
Controlled Vocabulary Terms
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Click here to see a list of all updates to this record from FB2010_08 and on.
FlyBase Computed Cytological Location
Experimentally Determined Cytological Location
Experimentally Determined Recombination Data
Location Left of (cM) Right of (cM) Notes Comments on Gene
Model Gene model reviewed during 5.44
Annotated Transcripts Additional Transcript Data
& Comments Reported size (kB) Comments External Data Crossreferences Annotated
Polypeptides Additional Polypeptide
Data & Comments Reported size (kDa) Comments External Data Linkouts Crossreferences
domains - A database of protein families, domains, and functional sites
DDBJ / EMBL / GenBank UniProtKB/Swiss-Prot UniProtKB/TrEMBL Mapped Features have been reorganized, please
see this article for details. Additional mapped features and mutations can
be found on GBrowse or related reports. Linkouts Crossreferences Comment: clustered apical distribution
Comment: maternally deposited
Comment: maternally deposited
Comment: reported as procephalic ectoderm anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalon primordium
Comment: reported as procephalon primordium
Comment: reported as procephalon primordium
Comment: reported as procephalon primordium
Comment: reported as procephalon primordium
Comment: reported as procephalon primordium
Additional Descriptive Data
smo transcripts are expressed at all developmental stages.
smo transcripts are detected in embryos, larvae, and pupae on northern blots. They are most abundant in early embryos and adult females.
Marker for Subcellular Localization CV Term
Additional Descriptive Data
Marker for Subcellular Localization (GO Cellular Component) Associated Tools
Reference
Linear, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
216.45
Larval Midgut
62.7
Larval Hindgut
130.2
Larval Malpighian Tubules
126
Larval Fat Body
92.7
Larval Salivary Gland
72.9
Larval Trachea
403.525
Larval Carcass
159.275
Adult Head
75.4
Adult Eye
90.7
Adult Brain
83.1
Adult Thoracic-Abdominal Ganglion
77
Adult Crop
161
Adult Midgut
53.7
Adult Hindgut
106.1
Adult Malpighian Tubules
81
Adult Fat Body
170.6
Adult Salivary Gland
66.1
Adult Heart
141.375
Adult VirginFemale Spermatheca
145.7
Adult InseminatedFemale Spermatheca
168.1
Adult Ovary
340.9
Adult Testis
24.3
Adult Male Accessory Gland
70
Adult Carcass
96.4
Expression Level Scale
None
Low
Moderate
Linear, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
216.45
Larval Midgut
62.7
Larval Hindgut
130.2
Larval Malpighian Tubules
126
Larval Fat Body
92.7
Larval Salivary Gland
72.9
Larval Trachea
403.525
Larval Carcass
159.275
Adult Head
75.4
Adult Eye
90.7
Adult Brain
83.1
Adult Thoracic-Abdominal Ganglion
77
Adult Crop
161
Adult Midgut
53.7
Adult Hindgut
106.1
Adult Malpighian Tubules
81
Adult Fat Body
170.6
Adult Salivary Gland
66.1
Adult Heart
141.375
Adult VirginFemale Spermatheca
145.7
Adult InseminatedFemale Spermatheca
168.1
Adult Ovary
340.9
Adult Testis
24.3
Adult Male Accessory Gland
70
Adult Carcass
96.4
Expression Level Scale
None
Low
Moderate
High
Linear, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
216.45
Larval Midgut
62.7
Larval Hindgut
130.2
Larval Malpighian Tubules
126
Larval Fat Body
92.7
Larval Salivary Gland
72.9
Larval Trachea
403.525
Larval Carcass
159.275
Adult Head
75.4
Adult Eye
90.7
Adult Brain
83.1
Adult Thoracic-Abdominal Ganglion
77
Adult Crop
161
Adult Midgut
53.7
Adult Hindgut
106.1
Adult Malpighian Tubules
81
Adult Fat Body
170.6
Adult Salivary Gland
66.1
Adult Heart
141.375
Adult VirginFemale Spermatheca
145.7
Adult InseminatedFemale Spermatheca
168.1
Adult Ovary
340.9
Adult Testis
24.3
Adult Male Accessory Gland
70
Adult Carcass
96.4
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
216.45
Larval Midgut
62.7
Larval Hindgut
130.2
Larval Malpighian Tubules
126
Larval Fat Body
92.7
Larval Salivary Gland
72.9
Larval Trachea
403.525
Larval Carcass
159.275
Adult Head
75.4
Adult Eye
90.7
Adult Brain
83.1
Adult Thoracic-Abdominal Ganglion
77
Adult Crop
161
Adult Midgut
53.7
Adult Hindgut
106.1
Adult Malpighian Tubules
81
Adult Fat Body
170.6
Adult Salivary Gland
66.1
Adult Heart
141.375
Adult VirginFemale Spermatheca
145.7
Adult InseminatedFemale Spermatheca
168.1
Adult Ovary
340.9
Adult Testis
24.3
Adult Male Accessory Gland
70
Adult Carcass
96.4
Expression Level Scale
Very high
log, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
216.45
Larval Midgut
62.7
Larval Hindgut
130.2
Larval Malpighian Tubules
126
Larval Fat Body
92.7
Larval Salivary Gland
72.9
Larval Trachea
403.525
Larval Carcass
159.275
Adult Head
75.4
Adult Eye
90.7
Adult Brain
83.1
Adult Thoracic-Abdominal Ganglion
77
Adult Crop
161
Adult Midgut
53.7
Adult Hindgut
106.1
Adult Malpighian Tubules
81
Adult Fat Body
170.6
Adult Salivary Gland
66.1
Adult Heart
141.375
Adult VirginFemale Spermatheca
145.7
Adult InseminatedFemale Spermatheca
168.1
Adult Ovary
340.9
Adult Testis
24.3
Adult Male Accessory Gland
70
Adult Carcass
96.4
Expression Level Scale
None
Low
Moderate
High
log, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
216.45
Larval Midgut
62.7
Larval Hindgut
130.2
Larval Malpighian Tubules
126
Larval Fat Body
92.7
Larval Salivary Gland
72.9
Larval Trachea
403.525
Larval Carcass
159.275
Adult Head
75.4
Adult Eye
90.7
Adult Brain
83.1
Adult Thoracic-Abdominal Ganglion
77
Adult Crop
161
Adult Midgut
53.7
Adult Hindgut
106.1
Adult Malpighian Tubules
81
Adult Fat Body
170.6
Adult Salivary Gland
66.1
Adult Heart
141.375
Adult VirginFemale Spermatheca
145.7
Adult InseminatedFemale Spermatheca
168.1
Adult Ovary
340.9
Adult Testis
24.3
Adult Male Accessory Gland
70
Adult Carcass
96.4
Expression Level Scale
None
Low
Moderate
High
log, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
216.45
Larval Midgut
62.7
Larval Hindgut
130.2
Larval Malpighian Tubules
126
Larval Fat Body
92.7
Larval Salivary Gland
72.9
Larval Trachea
403.525
Larval Carcass
159.275
Adult Head
75.4
Adult Eye
90.7
Adult Brain
83.1
Adult Thoracic-Abdominal Ganglion
77
Adult Crop
161
Adult Midgut
53.7
Adult Hindgut
106.1
Adult Malpighian Tubules
81
Adult Fat Body
170.6
Adult Salivary Gland
66.1
Adult Heart
141.375
Adult VirginFemale Spermatheca
145.7
Adult InseminatedFemale Spermatheca
168.1
Adult Ovary
340.9
Adult Testis
24.3
Adult Male Accessory Gland
70
Adult Carcass
96.4
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
216.45
Larval Midgut
62.7
Larval Hindgut
130.2
Larval Malpighian Tubules
126
Larval Fat Body
92.7
Larval Salivary Gland
72.9
Larval Trachea
403.525
Larval Carcass
159.275
Adult Head
75.4
Adult Eye
90.7
Adult Brain
83.1
Adult Thoracic-Abdominal Ganglion
77
Adult Crop
161
Adult Midgut
53.7
Adult Hindgut
106.1
Adult Malpighian Tubules
81
Adult Fat Body
170.6
Adult Salivary Gland
66.1
Adult Heart
141.375
Adult VirginFemale Spermatheca
145.7
Adult InseminatedFemale Spermatheca
168.1
Adult Ovary
340.9
Adult Testis
24.3
Adult Male Accessory Gland
70
Adult Carcass
96.4
Expression Level Scale
None
Low
Moderate
High
Very high
Heatmap
Tissue
Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass
FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level
Tissue
Adult Expression Level
NA Head
75.4
NA Eye
90.7
NA Brain
83.1
216.45 Central Nervous System
NA
NA Thoracic-Abdominal Ganglion
77
NA Crop
161
62.7 Midgut
53.7
130.2 Hindgut
106.1
126 Malpighian Tubules
81
92.7 Fat Body
170.6
72.9 Salivary Gland
66.1
NA Heart
141.375
403.525 Trachea
NA
NA VirginFemale Spermatheca
145.7
NA InseminatedFemale Spermatheca
168.1
NA Ovary
340.9
NA Testis
24.3
NA Male Accessory Gland
70
159.275 Carcass
96.4
Linear, scaled to maximum expression level
Tissue
Expression Level
imaginal disc, larvae L3 wandering
15
central nervous system, larvae L3
12
central nervous system, pupae P8
5
head, virgin 1-day female
3
head, virgin 4-day female
3
head, virgin 20-day female
3
head, mated 1-day female
3
head, mated 4-day female
3
head, mated 20-day female
3
head, mated 1-day male
5
head, mated 4-day male
5
head, mated 20-day male
6
salivary gland, larvae L3 wandering
1
salivary gland, white prepupae
2
digestive system, larvae L3 wandering
3
digestive system, 1-day adult
1
digestive system, 4-day adult
3
digestive system, 20-day adult
2
fat body, larvae L3 wandering
2
fat body, white prepupae
5
fat body, pupae P8
8
carcass, larvae L3 wandering
12
carcass, 1-day adult
3
carcass, 4-day adult
4
carcass, 20-day adult
4
ovary, virgin 4-day female
36
ovary, mated 4-day female
23
testis, mated 4-day male
1
accessory gland, mated 4-day male
2
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to Moderate expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
15
central nervous system, larvae L3
12
central nervous system, pupae P8
5
head, virgin 1-day female
3
head, virgin 4-day female
3
head, virgin 20-day female
3
head, mated 1-day female
3
head, mated 4-day female
3
head, mated 20-day female
3
head, mated 1-day male
5
head, mated 4-day male
5
head, mated 20-day male
6
salivary gland, larvae L3 wandering
1
salivary gland, white prepupae
2
digestive system, larvae L3 wandering
3
digestive system, 1-day adult
1
digestive system, 4-day adult
3
digestive system, 20-day adult
2
fat body, larvae L3 wandering
2
fat body, white prepupae
5
fat body, pupae P8
8
carcass, larvae L3 wandering
12
carcass, 1-day adult
3
carcass, 4-day adult
4
carcass, 20-day adult
4
ovary, virgin 4-day female
(36)
ovary, mated 4-day female
23
testis, mated 4-day male
1
accessory gland, mated 4-day male
2
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
15
central nervous system, larvae L3
12
central nervous system, pupae P8
5
head, virgin 1-day female
3
head, virgin 4-day female
3
head, virgin 20-day female
3
head, mated 1-day female
3
head, mated 4-day female
3
head, mated 20-day female
3
head, mated 1-day male
5
head, mated 4-day male
5
head, mated 20-day male
6
salivary gland, larvae L3 wandering
1
salivary gland, white prepupae
2
digestive system, larvae L3 wandering
3
digestive system, 1-day adult
1
digestive system, 4-day adult
3
digestive system, 20-day adult
2
fat body, larvae L3 wandering
2
fat body, white prepupae
5
fat body, pupae P8
8
carcass, larvae L3 wandering
12
carcass, 1-day adult
3
carcass, 4-day adult
4
carcass, 20-day adult
4
ovary, virgin 4-day female
36
ovary, mated 4-day female
23
testis, mated 4-day male
1
accessory gland, mated 4-day male
2
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
15
central nervous system, larvae L3
12
central nervous system, pupae P8
5
head, virgin 1-day female
3
head, virgin 4-day female
3
head, virgin 20-day female
3
head, mated 1-day female
3
head, mated 4-day female
3
head, mated 20-day female
3
head, mated 1-day male
5
head, mated 4-day male
5
head, mated 20-day male
6
salivary gland, larvae L3 wandering
1
salivary gland, white prepupae
2
digestive system, larvae L3 wandering
3
digestive system, 1-day adult
1
digestive system, 4-day adult
3
digestive system, 20-day adult
2
fat body, larvae L3 wandering
2
fat body, white prepupae
5
fat body, pupae P8
8
carcass, larvae L3 wandering
12
carcass, 1-day adult
3
carcass, 4-day adult
4
carcass, 20-day adult
4
ovary, virgin 4-day female
36
ovary, mated 4-day female
23
testis, mated 4-day male
1
accessory gland, mated 4-day male
2
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Tissue
Expression Level
imaginal disc, larvae L3 wandering
15
central nervous system, larvae L3
12
central nervous system, pupae P8
5
head, virgin 1-day female
3
head, virgin 4-day female
3
head, virgin 20-day female
3
head, mated 1-day female
3
head, mated 4-day female
3
head, mated 20-day female
3
head, mated 1-day male
5
head, mated 4-day male
5
head, mated 20-day male
6
salivary gland, larvae L3 wandering
1
salivary gland, white prepupae
2
digestive system, larvae L3 wandering
3
digestive system, 1-day adult
1
digestive system, 4-day adult
3
digestive system, 20-day adult
2
fat body, larvae L3 wandering
2
fat body, white prepupae
5
fat body, pupae P8
8
carcass, larvae L3 wandering
12
carcass, 1-day adult
3
carcass, 4-day adult
4
carcass, 20-day adult
4
ovary, virgin 4-day female
36
ovary, mated 4-day female
23
testis, mated 4-day male
1
accessory gland, mated 4-day male
2
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
log, scaled to Moderate expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
15
central nervous system, larvae L3
12
central nervous system, pupae P8
5
head, virgin 1-day female
3
head, virgin 4-day female
3
head, virgin 20-day female
3
head, mated 1-day female
3
head, mated 4-day female
3
head, mated 20-day female
3
head, mated 1-day male
5
head, mated 4-day male
5
head, mated 20-day male
6
salivary gland, larvae L3 wandering
1
salivary gland, white prepupae
2
digestive system, larvae L3 wandering
3
digestive system, 1-day adult
1
digestive system, 4-day adult
3
digestive system, 20-day adult
2
fat body, larvae L3 wandering
2
fat body, white prepupae
5
fat body, pupae P8
8
carcass, larvae L3 wandering
12
carcass, 1-day adult
3
carcass, 4-day adult
4
carcass, 20-day adult
4
ovary, virgin 4-day female
(36)
ovary, mated 4-day female
23
testis, mated 4-day male
1
accessory gland, mated 4-day male
2
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
15
central nervous system, larvae L3
12
central nervous system, pupae P8
5
head, virgin 1-day female
3
head, virgin 4-day female
3
head, virgin 20-day female
3
head, mated 1-day female
3
head, mated 4-day female
3
head, mated 20-day female
3
head, mated 1-day male
5
head, mated 4-day male
5
head, mated 20-day male
6
salivary gland, larvae L3 wandering
1
salivary gland, white prepupae
2
digestive system, larvae L3 wandering
3
digestive system, 1-day adult
1
digestive system, 4-day adult
3
digestive system, 20-day adult
2
fat body, larvae L3 wandering
2
fat body, white prepupae
5
fat body, pupae P8
8
carcass, larvae L3 wandering
12
carcass, 1-day adult
3
carcass, 4-day adult
4
carcass, 20-day adult
4
ovary, virgin 4-day female
36
ovary, mated 4-day female
23
testis, mated 4-day male
1
accessory gland, mated 4-day male
2
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
15
central nervous system, larvae L3
12
central nervous system, pupae P8
5
head, virgin 1-day female
3
head, virgin 4-day female
3
head, virgin 20-day female
3
head, mated 1-day female
3
head, mated 4-day female
3
head, mated 20-day female
3
head, mated 1-day male
5
head, mated 4-day male
5
head, mated 20-day male
6
salivary gland, larvae L3 wandering
1
salivary gland, white prepupae
2
digestive system, larvae L3 wandering
3
digestive system, 1-day adult
1
digestive system, 4-day adult
3
digestive system, 20-day adult
2
fat body, larvae L3 wandering
2
fat body, white prepupae
5
fat body, pupae P8
8
carcass, larvae L3 wandering
12
carcass, 1-day adult
3
carcass, 4-day adult
4
carcass, 20-day adult
4
ovary, virgin 4-day female
36
ovary, mated 4-day female
23
testis, mated 4-day male
1
accessory gland, mated 4-day male
2
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Tissue
Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male
Linear, scaled to maximum expression level
Developmental Stage
Expression Level
embryo 00-02hr
25
embryo 02-04hr
53
embryo 04-06hr
42
embryo 06-08hr
23
embryo 08-10hr
23
embryo 10-12hr
13
embryo 12-14hr
16
embryo 14-16hr
9
embryo 16-18hr
8
embryo 18-20hr
4
embryo 20-22hr
9
embryo 22-24hr
10
larva L1
4
larva L2
3
larva L3 12hr old
3
larva L3 puffstage 1-2
5
larva L3 puffstage 3-6
8
larva L3 puffstage 7-9
11
white prepupae new
12
white prepupae 12hr
10
white prepupae 24hr
12
pupae 2d postWPP
7
pupae 3d postWPP
3
pupae 4d postWPP
2
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
7
adult female 05day
17
adult female 30day
17
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Linear, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
25
embryo 02-04hr
(53)
embryo 04-06hr
(42)
embryo 06-08hr
23
embryo 08-10hr
23
embryo 10-12hr
13
embryo 12-14hr
16
embryo 14-16hr
9
embryo 16-18hr
8
embryo 18-20hr
4
embryo 20-22hr
9
embryo 22-24hr
10
larva L1
4
larva L2
3
larva L3 12hr old
3
larva L3 puffstage 1-2
5
larva L3 puffstage 3-6
8
larva L3 puffstage 7-9
11
white prepupae new
12
white prepupae 12hr
10
white prepupae 24hr
12
pupae 2d postWPP
7
pupae 3d postWPP
3
pupae 4d postWPP
2
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
7
adult female 05day
17
adult female 30day
17
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
25
embryo 02-04hr
53
embryo 04-06hr
42
embryo 06-08hr
23
embryo 08-10hr
23
embryo 10-12hr
13
embryo 12-14hr
16
embryo 14-16hr
9
embryo 16-18hr
8
embryo 18-20hr
4
embryo 20-22hr
9
embryo 22-24hr
10
larva L1
4
larva L2
3
larva L3 12hr old
3
larva L3 puffstage 1-2
5
larva L3 puffstage 3-6
8
larva L3 puffstage 7-9
11
white prepupae new
12
white prepupae 12hr
10
white prepupae 24hr
12
pupae 2d postWPP
7
pupae 3d postWPP
3
pupae 4d postWPP
2
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
7
adult female 05day
17
adult female 30day
17
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
25
embryo 02-04hr
53
embryo 04-06hr
42
embryo 06-08hr
23
embryo 08-10hr
23
embryo 10-12hr
13
embryo 12-14hr
16
embryo 14-16hr
9
embryo 16-18hr
8
embryo 18-20hr
4
embryo 20-22hr
9
embryo 22-24hr
10
larva L1
4
larva L2
3
larva L3 12hr old
3
larva L3 puffstage 1-2
5
larva L3 puffstage 3-6
8
larva L3 puffstage 7-9
11
white prepupae new
12
white prepupae 12hr
10
white prepupae 24hr
12
pupae 2d postWPP
7
pupae 3d postWPP
3
pupae 4d postWPP
2
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
7
adult female 05day
17
adult female 30day
17
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Developmental Stage
Expression Level
embryo 00-02hr
25
embryo 02-04hr
53
embryo 04-06hr
42
embryo 06-08hr
23
embryo 08-10hr
23
embryo 10-12hr
13
embryo 12-14hr
16
embryo 14-16hr
9
embryo 16-18hr
8
embryo 18-20hr
4
embryo 20-22hr
9
embryo 22-24hr
10
larva L1
4
larva L2
3
larva L3 12hr old
3
larva L3 puffstage 1-2
5
larva L3 puffstage 3-6
8
larva L3 puffstage 7-9
11
white prepupae new
12
white prepupae 12hr
10
white prepupae 24hr
12
pupae 2d postWPP
7
pupae 3d postWPP
3
pupae 4d postWPP
2
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
7
adult female 05day
17
adult female 30day
17
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
log, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
25
embryo 02-04hr
(53)
embryo 04-06hr
(42)
embryo 06-08hr
23
embryo 08-10hr
23
embryo 10-12hr
13
embryo 12-14hr
16
embryo 14-16hr
9
embryo 16-18hr
8
embryo 18-20hr
4
embryo 20-22hr
9
embryo 22-24hr
10
larva L1
4
larva L2
3
larva L3 12hr old
3
larva L3 puffstage 1-2
5
larva L3 puffstage 3-6
8
larva L3 puffstage 7-9
11
white prepupae new
12
white prepupae 12hr
10
white prepupae 24hr
12
pupae 2d postWPP
7
pupae 3d postWPP
3
pupae 4d postWPP
2
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
7
adult female 05day
17
adult female 30day
17
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
25
embryo 02-04hr
53
embryo 04-06hr
42
embryo 06-08hr
23
embryo 08-10hr
23
embryo 10-12hr
13
embryo 12-14hr
16
embryo 14-16hr
9
embryo 16-18hr
8
embryo 18-20hr
4
embryo 20-22hr
9
embryo 22-24hr
10
larva L1
4
larva L2
3
larva L3 12hr old
3
larva L3 puffstage 1-2
5
larva L3 puffstage 3-6
8
larva L3 puffstage 7-9
11
white prepupae new
12
white prepupae 12hr
10
white prepupae 24hr
12
pupae 2d postWPP
7
pupae 3d postWPP
3
pupae 4d postWPP
2
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
7
adult female 05day
17
adult female 30day
17
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
25
embryo 02-04hr
53
embryo 04-06hr
42
embryo 06-08hr
23
embryo 08-10hr
23
embryo 10-12hr
13
embryo 12-14hr
16
embryo 14-16hr
9
embryo 16-18hr
8
embryo 18-20hr
4
embryo 20-22hr
9
embryo 22-24hr
10
larva L1
4
larva L2
3
larva L3 12hr old
3
larva L3 puffstage 1-2
5
larva L3 puffstage 3-6
8
larva L3 puffstage 7-9
11
white prepupae new
12
white prepupae 12hr
10
white prepupae 24hr
12
pupae 2d postWPP
7
pupae 3d postWPP
3
pupae 4d postWPP
2
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
7
adult female 05day
17
adult female 30day
17
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Developmental Stage
Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day
Linear, scaled to maximum expression level
Cell Line
Expression Level
Schneider line 2 S2R+
19
Schneider line 2 Sg4
8
embryonic 1182-4H
15
embryonic GM2
7
embryonic Kc167
14
embryonic S1
9
embryonic S3
16
leg disc CME L1
9
wing disc CME-W2
15
wing disc ML-DmD8
23
wing disc ML-DmD9
15
wing disc ML-DmD16-c3
12
wing disc ML-DmD21
5
wing disc ML-DmD32
11
haltere disc ML-DmD17-c3
27
eye-antennal disc ML-DmD11
16
antennal disc ML-DmD20-c5
14
mixed discs ML-DmD4-c1
20
CNS ML-DmBG1-c1
22
CNS ML-DmBG2-c2
15
tumorous blood cells mbn2
19
ovary fGS/OSS
14
ovary OSC
16
ovary OSS
6
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to Moderate expression
Cell Line
Expression Level
Schneider line 2 S2R+
19
Schneider line 2 Sg4
8
embryonic 1182-4H
15
embryonic GM2
7
embryonic Kc167
14
embryonic S1
9
embryonic S3
16
leg disc CME L1
9
wing disc CME-W2
15
wing disc ML-DmD8
23
wing disc ML-DmD9
15
wing disc ML-DmD16-c3
12
wing disc ML-DmD21
5
wing disc ML-DmD32
11
haltere disc ML-DmD17-c3
(27)
eye-antennal disc ML-DmD11
16
antennal disc ML-DmD20-c5
14
mixed discs ML-DmD4-c1
20
CNS ML-DmBG1-c1
22
CNS ML-DmBG2-c2
15
tumorous blood cells mbn2
19
ovary fGS/OSS
14
ovary OSC
16
ovary OSS
6
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Cell Line
Expression Level
Schneider line 2 S2R+
19
Schneider line 2 Sg4
8
embryonic 1182-4H
15
embryonic GM2
7
embryonic Kc167
14
embryonic S1
9
embryonic S3
16
leg disc CME L1
9
wing disc CME-W2
15
wing disc ML-DmD8
23
wing disc ML-DmD9
15
wing disc ML-DmD16-c3
12
wing disc ML-DmD21
5
wing disc ML-DmD32
11
haltere disc ML-DmD17-c3
27
eye-antennal disc ML-DmD11
16
antennal disc ML-DmD20-c5
14
mixed discs ML-DmD4-c1
20
CNS ML-DmBG1-c1
22
CNS ML-DmBG2-c2
15
tumorous blood cells mbn2
19
ovary fGS/OSS
14
ovary OSC
16
ovary OSS
6
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Cell Line
Expression Level
Schneider line 2 S2R+
19
Schneider line 2 Sg4
8
embryonic 1182-4H
15
embryonic GM2
7
embryonic Kc167
14
embryonic S1
9
embryonic S3
16
leg disc CME L1
9
wing disc CME-W2
15
wing disc ML-DmD8
23
wing disc ML-DmD9
15
wing disc ML-DmD16-c3
12
wing disc ML-DmD21
5
wing disc ML-DmD32
11
haltere disc ML-DmD17-c3
27
eye-antennal disc ML-DmD11
16
antennal disc ML-DmD20-c5
14
mixed discs ML-DmD4-c1
20
CNS ML-DmBG1-c1
22
CNS ML-DmBG2-c2
15
tumorous blood cells mbn2
19
ovary fGS/OSS
14
ovary OSC
16
ovary OSS
6
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Cell Line
Expression Level
Schneider line 2 S2R+
19
Schneider line 2 Sg4
8
embryonic 1182-4H
15
embryonic GM2
7
embryonic Kc167
14
embryonic S1
9
embryonic S3
16
leg disc CME L1
9
wing disc CME-W2
15
wing disc ML-DmD8
23
wing disc ML-DmD9
15
wing disc ML-DmD16-c3
12
wing disc ML-DmD21
5
wing disc ML-DmD32
11
haltere disc ML-DmD17-c3
27
eye-antennal disc ML-DmD11
16
antennal disc ML-DmD20-c5
14
mixed discs ML-DmD4-c1
20
CNS ML-DmBG1-c1
22
CNS ML-DmBG2-c2
15
tumorous blood cells mbn2
19
ovary fGS/OSS
14
ovary OSC
16
ovary OSS
6
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to Moderate expression
Cell Line
Expression Level
Schneider line 2 S2R+
19
Schneider line 2 Sg4
8
embryonic 1182-4H
15
embryonic GM2
7
embryonic Kc167
14
embryonic S1
9
embryonic S3
16
leg disc CME L1
9
wing disc CME-W2
15
wing disc ML-DmD8
23
wing disc ML-DmD9
15
wing disc ML-DmD16-c3
12
wing disc ML-DmD21
5
wing disc ML-DmD32
11
haltere disc ML-DmD17-c3
27
eye-antennal disc ML-DmD11
16
antennal disc ML-DmD20-c5
14
mixed discs ML-DmD4-c1
20
CNS ML-DmBG1-c1
22
CNS ML-DmBG2-c2
15
tumorous blood cells mbn2
19
ovary fGS/OSS
14
ovary OSC
16
ovary OSS
6
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Cell Line
Expression Level
Schneider line 2 S2R+
19
Schneider line 2 Sg4
8
embryonic 1182-4H
15
embryonic GM2
7
embryonic Kc167
14
embryonic S1
9
embryonic S3
16
leg disc CME L1
9
wing disc CME-W2
15
wing disc ML-DmD8
23
wing disc ML-DmD9
15
wing disc ML-DmD16-c3
12
wing disc ML-DmD21
5
wing disc ML-DmD32
11
haltere disc ML-DmD17-c3
27
eye-antennal disc ML-DmD11
16
antennal disc ML-DmD20-c5
14
mixed discs ML-DmD4-c1
20
CNS ML-DmBG1-c1
22
CNS ML-DmBG2-c2
15
tumorous blood cells mbn2
19
ovary fGS/OSS
14
ovary OSC
16
ovary OSS
6
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Cell Line
Expression Level
Schneider line 2 S2R+
19
Schneider line 2 Sg4
8
embryonic 1182-4H
15
embryonic GM2
7
embryonic Kc167
14
embryonic S1
9
embryonic S3
16
leg disc CME L1
9
wing disc CME-W2
15
wing disc ML-DmD8
23
wing disc ML-DmD9
15
wing disc ML-DmD16-c3
12
wing disc ML-DmD21
5
wing disc ML-DmD32
11
haltere disc ML-DmD17-c3
27
eye-antennal disc ML-DmD11
16
antennal disc ML-DmD20-c5
14
mixed discs ML-DmD4-c1
20
CNS ML-DmBG1-c1
22
CNS ML-DmBG2-c2
15
tumorous blood cells mbn2
19
ovary fGS/OSS
14
ovary OSC
16
ovary OSS
6
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Cell Line
Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS
Linear, scaled to maximum expression level
Treatment
Expression Level
extended cold, 4-day adult
4
cold shock, 4-day adult
3
heat shock, 4-day adult
8
Cadmium 50 mM 6 hrs, larvae L3
2
Cadmium 50 mM 12 hrs, larvae L3
7
Cadmium 50 mM 48 hrs, 4-day adult
8
Cadmium 100 mM 48 hrs, 4-day adult
21
Copper 0.5 mM 12 hrs, larvae L3
2
Copper 15 mM 48 hrs, 4-day adult
19
Zinc 5 mM 12 hrs, larvae L3
2
Zinc 4.5 mM 48 hrs, 4-day adult
7
Ethanol 2.5% 3 hrs, larvae L3
15
Ethanol 5% 3 hrs, larvae L3
5
Ethanol 10% 3 hrs, larvae L3
3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
5
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
17
Caffeine 25 mg/ml 48 hrs, 4-day adult
5
Paraquat 5 mM 48 hrs, 4-day adult
13
Paraquat 10 mM 48 hrs, 4-day adult
9
Rotenone 2 μg 12 hrs, larvae L3
5
Rotenone 8 μg 12 hrs, larvae L3
2
Expression Level Scale
Very low
Low
Moderate
Linear, scaled to Moderate expression
Treatment
Expression Level
extended cold, 4-day adult
4
cold shock, 4-day adult
3
heat shock, 4-day adult
8
Cadmium 50 mM 6 hrs, larvae L3
2
Cadmium 50 mM 12 hrs, larvae L3
7
Cadmium 50 mM 48 hrs, 4-day adult
8
Cadmium 100 mM 48 hrs, 4-day adult
21
Copper 0.5 mM 12 hrs, larvae L3
2
Copper 15 mM 48 hrs, 4-day adult
19
Zinc 5 mM 12 hrs, larvae L3
2
Zinc 4.5 mM 48 hrs, 4-day adult
7
Ethanol 2.5% 3 hrs, larvae L3
15
Ethanol 5% 3 hrs, larvae L3
5
Ethanol 10% 3 hrs, larvae L3
3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
5
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
17
Caffeine 25 mg/ml 48 hrs, 4-day adult
5
Paraquat 5 mM 48 hrs, 4-day adult
13
Paraquat 10 mM 48 hrs, 4-day adult
9
Rotenone 2 μg 12 hrs, larvae L3
5
Rotenone 8 μg 12 hrs, larvae L3
2
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Treatment
Expression Level
extended cold, 4-day adult
4
cold shock, 4-day adult
3
heat shock, 4-day adult
8
Cadmium 50 mM 6 hrs, larvae L3
2
Cadmium 50 mM 12 hrs, larvae L3
7
Cadmium 50 mM 48 hrs, 4-day adult
8
Cadmium 100 mM 48 hrs, 4-day adult
21
Copper 0.5 mM 12 hrs, larvae L3
2
Copper 15 mM 48 hrs, 4-day adult
19
Zinc 5 mM 12 hrs, larvae L3
2
Zinc 4.5 mM 48 hrs, 4-day adult
7
Ethanol 2.5% 3 hrs, larvae L3
15
Ethanol 5% 3 hrs, larvae L3
5
Ethanol 10% 3 hrs, larvae L3
3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
5
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
17
Caffeine 25 mg/ml 48 hrs, 4-day adult
5
Paraquat 5 mM 48 hrs, 4-day adult
13
Paraquat 10 mM 48 hrs, 4-day adult
9
Rotenone 2 μg 12 hrs, larvae L3
5
Rotenone 8 μg 12 hrs, larvae L3
2
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Treatment
Expression Level
extended cold, 4-day adult
4
cold shock, 4-day adult
3
heat shock, 4-day adult
8
Cadmium 50 mM 6 hrs, larvae L3
2
Cadmium 50 mM 12 hrs, larvae L3
7
Cadmium 50 mM 48 hrs, 4-day adult
8
Cadmium 100 mM 48 hrs, 4-day adult
21
Copper 0.5 mM 12 hrs, larvae L3
2
Copper 15 mM 48 hrs, 4-day adult
19
Zinc 5 mM 12 hrs, larvae L3
2
Zinc 4.5 mM 48 hrs, 4-day adult
7
Ethanol 2.5% 3 hrs, larvae L3
15
Ethanol 5% 3 hrs, larvae L3
5
Ethanol 10% 3 hrs, larvae L3
3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
5
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
17
Caffeine 25 mg/ml 48 hrs, 4-day adult
5
Paraquat 5 mM 48 hrs, 4-day adult
13
Paraquat 10 mM 48 hrs, 4-day adult
9
Rotenone 2 μg 12 hrs, larvae L3
5
Rotenone 8 μg 12 hrs, larvae L3
2
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Treatment
Expression Level
extended cold, 4-day adult
4
cold shock, 4-day adult
3
heat shock, 4-day adult
8
Cadmium 50 mM 6 hrs, larvae L3
2
Cadmium 50 mM 12 hrs, larvae L3
7
Cadmium 50 mM 48 hrs, 4-day adult
8
Cadmium 100 mM 48 hrs, 4-day adult
21
Copper 0.5 mM 12 hrs, larvae L3
2
Copper 15 mM 48 hrs, 4-day adult
19
Zinc 5 mM 12 hrs, larvae L3
2
Zinc 4.5 mM 48 hrs, 4-day adult
7
Ethanol 2.5% 3 hrs, larvae L3
15
Ethanol 5% 3 hrs, larvae L3
5
Ethanol 10% 3 hrs, larvae L3
3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
5
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
17
Caffeine 25 mg/ml 48 hrs, 4-day adult
5
Paraquat 5 mM 48 hrs, 4-day adult
13
Paraquat 10 mM 48 hrs, 4-day adult
9
Rotenone 2 μg 12 hrs, larvae L3
5
Rotenone 8 μg 12 hrs, larvae L3
2
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to Moderate expression
Treatment
Expression Level
extended cold, 4-day adult
4
cold shock, 4-day adult
3
heat shock, 4-day adult
8
Cadmium 50 mM 6 hrs, larvae L3
2
Cadmium 50 mM 12 hrs, larvae L3
7
Cadmium 50 mM 48 hrs, 4-day adult
8
Cadmium 100 mM 48 hrs, 4-day adult
21
Copper 0.5 mM 12 hrs, larvae L3
2
Copper 15 mM 48 hrs, 4-day adult
19
Zinc 5 mM 12 hrs, larvae L3
2
Zinc 4.5 mM 48 hrs, 4-day adult
7
Ethanol 2.5% 3 hrs, larvae L3
15
Ethanol 5% 3 hrs, larvae L3
5
Ethanol 10% 3 hrs, larvae L3
3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
5
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
17
Caffeine 25 mg/ml 48 hrs, 4-day adult
5
Paraquat 5 mM 48 hrs, 4-day adult
13
Paraquat 10 mM 48 hrs, 4-day adult
9
Rotenone 2 μg 12 hrs, larvae L3
5
Rotenone 8 μg 12 hrs, larvae L3
2
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Treatment
Expression Level
extended cold, 4-day adult
4
cold shock, 4-day adult
3
heat shock, 4-day adult
8
Cadmium 50 mM 6 hrs, larvae L3
2
Cadmium 50 mM 12 hrs, larvae L3
7
Cadmium 50 mM 48 hrs, 4-day adult
8
Cadmium 100 mM 48 hrs, 4-day adult
21
Copper 0.5 mM 12 hrs, larvae L3
2
Copper 15 mM 48 hrs, 4-day adult
19
Zinc 5 mM 12 hrs, larvae L3
2
Zinc 4.5 mM 48 hrs, 4-day adult
7
Ethanol 2.5% 3 hrs, larvae L3
15
Ethanol 5% 3 hrs, larvae L3
5
Ethanol 10% 3 hrs, larvae L3
3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
5
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
17
Caffeine 25 mg/ml 48 hrs, 4-day adult
5
Paraquat 5 mM 48 hrs, 4-day adult
13
Paraquat 10 mM 48 hrs, 4-day adult
9
Rotenone 2 μg 12 hrs, larvae L3
5
Rotenone 8 μg 12 hrs, larvae L3
2
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Treatment
Expression Level
extended cold, 4-day adult
4
cold shock, 4-day adult
3
heat shock, 4-day adult
8
Cadmium 50 mM 6 hrs, larvae L3
2
Cadmium 50 mM 12 hrs, larvae L3
7
Cadmium 50 mM 48 hrs, 4-day adult
8
Cadmium 100 mM 48 hrs, 4-day adult
21
Copper 0.5 mM 12 hrs, larvae L3
2
Copper 15 mM 48 hrs, 4-day adult
19
Zinc 5 mM 12 hrs, larvae L3
2
Zinc 4.5 mM 48 hrs, 4-day adult
7
Ethanol 2.5% 3 hrs, larvae L3
15
Ethanol 5% 3 hrs, larvae L3
5
Ethanol 10% 3 hrs, larvae L3
3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
5
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
17
Caffeine 25 mg/ml 48 hrs, 4-day adult
5
Paraquat 5 mM 48 hrs, 4-day adult
13
Paraquat 10 mM 48 hrs, 4-day adult
9
Rotenone 2 μg 12 hrs, larvae L3
5
Rotenone 8 μg 12 hrs, larvae L3
2
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Treatment
Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3
A cluster of genes with similar mRNA expression dynamics across development.
Linkouts
- Cell culture data for RNAi and other high-throughput technologies
- Adult expression by tissue, using Affymetrix Dros2 array
adult abdomen & cuticle | anterior compartment
adult abdomen & microchaeta | anterior compartment
lamina & neuron | precursor | somatic clone
morphogenetic furrow & nucleus | somatic clone
photoreceptor cell & neuron
photoreceptor cell R8 & eye disc | somatic clone
For All Classical Alleles Show For All Alleles Carried on Transgenic
Constructs Show Allele of
smo Class Mutagen Stocks Known lesion smo5A.Scer\UAS.T:Zzzz\FLAG 1 Yes smodsRNA.Scer\UAS.WIZ 1 Yes smoGD577 1 Yes smoGL01472 1 Yes smoJF02363 1 Yes smo+t12.1 0 Yes smo+t6.2 0 Yes smo+t6.6 0 Yes smo+tMa 0 Yes smo+tvdHa 0 Yes smo222.Scer\UAS.T:Hsap\MYC 0 Yes smo222.tub.Scer\UAS.T:Zzzz\FLAG 0 Yes smo222.tub.Scer\UAS 0 Yes smo223.tub.Scer\UAS 0 Yes smo680.687.Ala.Act5C.T:Hsap\MYC 0 Yes smo680.687.Glu.Act5C.T:Hsap\MYC 0 Yes smo680.Ala.Act5C.T:Hsap\MYC 0 Yes smo680.Glu.Act5C.T:Hsap\MYC 0 Yes smo687.740.Ala.Act5C.T:Hsap\MYC 0 Yes smo687.740.Glu+otherV.VI.Ala.Act5C.T:Hsap\MYC 0 Yes smo687.740.Glu.Act5C.T:Hsap\MYC 0 Yes smo687.Ala.Act5C.T:Hsap\MYC 0 Yes smo687.Glu.Act5C.T:Hsap\MYC 0 Yes smo740.Ala.Act5C.T:Hsap\MYC 0 Yes smo740.Glu.Act5C.T:Hsap\MYC 0 Yes smoA479Y.Scer\UAS.T:Avic\GFP-EGFP 0 Yes smoAAA.tub.Scer\UAS 0 Yes smoAAD.Scer\UAS.T:Hsap\MYC 0 Yes smoAAS.Scer\UAS.T:Hsap\MYC 0 Yes smoAAS.tub.Scer\UAS 0 Yes smoAatII-ApaI 0 Yes smoAct5C.cOa.T:Hsap\MYC 0 Yes smoAct5C.PC 0 Yes smoAct5C.PL 0 Yes smoAct5C.T:Avic\GFP-EGFP 0 Yes smoAct5C.T:Hsap\MYC 0 Yes smoAct5C.T:Ivir\HA1 0 Yes smoAct5C.T:Rren\LUC 0 Yes smoADA.Scer\UAS.T:Hsap\MYC 0 Yes smoADD.Scer\UAS.T:Hsap\MYC 0 Yes smoAll.PKA.Ala.Act5C.T:Hsap\MYC 0 Yes smoAll.PKA.Glu.Act5C.T:Hsap\MYC 0 Yes smoAll.PKA.III.V.VI.VIII.Ala.Act5C.T:Hsap\MYC 0 Yes smoAll.PKA.III.V.VI.VIII.Glu.Act5C.T:Hsap\MYC 0 Yes smoALTΔC.Act5C 0 Yes smoAN1234.Scer\UAS 0 Yes smoAPKA.DACK1.Scer\UAS.T:Hsap\MYC 0 Yes smoAPKA.DDCK1.Scer\UAS.T:Hsap\MYC 0 Yes smoASA.Scer\UAS.T:Hsap\MYC 0 Yes smoASS.Scer\UAS.T:Hsap\MYC 0 Yes smoASS.tub.Scer\UAS 0 Yes smoC.Scer\UAS.T:Avic\GFP-CFP 0 Yes smoC.Scer\UAS.T:Avic\GFP-YFP 0 Yes smoC.Scer\UAS.T:Hsap\MYC,T:Uuuu\Myr4 0 Yes smoC.Scer\UAS.T:Hsap\MYC 0 Yes smoC.T:Sjap\GST 0 Yes smoC155Y 0 Yes smoC320A.Scer\UAS.T:Hsap\MYC 0 Yes smoC339A.Scer\UAS.T:Hsap\MYC 0 Yes smoC413A.Scer\UAS.T:Hsap\MYC 0 Yes smoCK1.Scer\FRT.Avic\GFP.y.Scer\UAS.T:Ivir\HA1 0 Yes smoCK1.Scer\UAS.T:Ivir\HA1 0 Yes smoCK2SA.Scer\UAS.T:Hsap\MYC 0 -- smoCK2SA.tub.Scer\UAS.T:Hsap\MYC 0 Yes smoCKI.Scer\UAS.T:Avic\GFP-CFP 0 Yes smoCKI.Scer\UAS.T:Zzzz\FLAG 0 Yes smoCT.556-730.Scer\UAS.T:Zzzz\FLAG,T:Myr-Src64B 0 Yes smoCT.556-818.Scer\UAS.T:Zzzz\FLAG,T:Myr-Src64B 0 Yes smoCT.730-1035.Scer\UAS.T:Zzzz\FLAG,T:Myr-Src64B 0 Yes smoCT.730-818.Scer\UAS.T:Zzzz\FLAG,T:Myr-Src64B 0 Yes smoCT.Scer\UAS.T:Zzzz\FLAG,T:Myr-Src64B 0 Yes smoCT.Scer\UAS.T:Zzzz\FLAG,T:TM-sev 0 Yes smoCT.Scer\UAS.T:Zzzz\FLAG 0 Yes smoCT.Δ625-730.Scer\UAS.T:Zzzz\FLAG,T:Myr-Src64B 0 Yes smoCT.Δ625-818.Scer\UAS.T:Zzzz\FLAG,T:Myr-Src64B 0 Yes smoDAA.Scer\UAS.T:Hsap\MYC 0 Yes smoDAD.Scer\UAS.T:Hsap\Myc 0 Yes smoDDA.Scer\UAS.T:Hsap\MYC 0 Yes smoDDD.Scer\UAS.T:Hsap\MYC 0 Yes smoDDS.Scer\UAS.T:Hsap\MYC 0 Yes smoDPKA.AACK1.Scer\UAS.T:Hsap\MYC 0 Yes smoDSD.Scer\UAS.T:Hsap\MYC 0 Yes smodsRNA.141-370 0 Yes smodsRNA.cIa 0 Yes smodsRNA.cKa 0 Yes smodsRNA.cLa 0 Yes smodsRNA.cRa 0 Yes smodsRNA.si.cKa 0 Yes smoDSS.Scer\UAS.T:Hsap\MYC 0 Yes smoGSK3.Scer\FRT.Avic\GFP.y.Scer\UAS.T:Ivir\HA1 0 Yes smoGSK3.Scer\UAS.T:Ivir\HA1 0 Yes smoI.Ala.Act5C.T:Hsap\MYC 0 Yes smoI.Glu.Act5C.T:Hsap\MYC 0 Yes smoI.II.Ala.Act5C.T:Hsap\MYC 0 Yes smoI.II.Glu.Act5C.T:Hsap\MYC 0 Yes smoI.II.III.IV.Ala.Act5C.T:Hsap\MYC 0 Yes smoI.II.III.IV.Glu.Act5C.T:Hsap\MYC 0 Yes smoI587A.Act5C 0 Yes smoII.Ala.Act5C.T:Hsap\MYC 0 Yes smoII.Glu.Act5C.T:Hsap\MYC 0 Yes smoIII.Ala.Act5C.T:Hsap\MYC 0 Yes smoIII.Glu.Act5C.T:Hsap\MYC 0 Yes smoIII.IV.Ala.Act5C.T:Hsap\MYC 0 Yes smoIII.IV.Glu.Act5C.T:Hsap\MYC 0 Yes smoIII.V.VI.Ala.Act5C.T:Hsap\MYC 0 Yes smoIII.V.VI.Ala.Scer\UAS.T:Avic\GFP 0 Yes smoIII.V.VI.Glu.Act5C.T:Hsap\MYC 0 Yes smoIII.V.VI.Glu.Scer\UAS.T:Avic\GFP 0 Yes smoIII.V.VI.VIII.Ala.Act5C.T:Hsap\MYC 0 Yes smoIII.V.VI.VIII.Glu.Act5C.T:Hsap\MYC 0 Yes smoIV.Ala.Act5C.T:Hsap\MYC 0 Yes smoIV.Glu.Act5C.T:Hsap\MYC 0 Yes smoK564A.Act5C 0 Yes smoK580Q.Scer\UAS.T:Zzzz\FLAG 0 Yes smoKCGΔC.Act5C 0 Yes smoL3C.Scer\UAS.T:Avic\GFP-CFP,T:Avic\GFP-YFP 0 Yes smoM1.Scer\UAS.T:Avic\GFP,T:Uuuu\KKDE 0 Yes smoM1.Scer\UAS.T:Avic\GFP 0 Yes smoM2.Scer\UAS.T:Avic\GFP,T:Uuuu\KKDE 0 Yes smoM2.Scer\UAS.T:Avic\GFP 0 Yes smoN.Scer\UAS.T:Avic\GFP-CFP 0 Yes smoN.Scer\UAS.T:Avic\GFP-YFP 0 Yes smoN.Scer\UAS.T:Hsap\MYC 0 Yes smoNIG.11561R 0 Yes smoPKA.Scer\FRT.Avic\GFP.y.Scer\UAS.T:Ivir\HA1 0 Yes smoPKA.Scer\UAS.T:Ivir\HA1 0 Yes smoPKA12.Scer\UAS 0 Yes smoPKA123.Scer\UAS.T:Avic\GFP-CFP 0 Yes smoPKA123.Scer\UAS.T:Zzzz\FLAG 0 Yes smoPKA23.Scer\UAS.T:Zzzz\FLAG 0 Yes smoPKA3.Scer\UAS.T:Zzzz\FLAG 0 Yes smoR565A.Act5C 0 Yes smoR646A.Act5C 0 Yes smoR647A.Act5C 0 Yes smoRA1.Scer\UAS 0 Yes smoRA12.Scer\UAS 0 Yes smoRA123.Scer\UAS 0 Yes smoRA1234.Scer\UAS 0 Yes smoRA124.Scer\UAS 0 Yes smoRA12D3.Scer\UAS 0 Yes smoRA12K34.Scer\UAS 0 Yes smoRA13.Scer\UAS 0 Yes smoRA14.Scer\UAS 0 Yes smoRA3.Scer\UAS 0 Yes smoRRNΔC.Act5C 0 Yes smoS558A.Act5C 0 Yes smoS559A.Act5C 0 Yes smoS604A.T606A.Act5C 0 Yes smoS626A.S627A.Act5C 0 Yes smoS633A.S634A.Act5C 0 Yes smoSAA.Scer\UAS.T:Hsap\MYC 0 Yes smoSAA.tub.Scer\UAS 0 Yes smoSAS.Scer\UAS.T:Hsap\MYC 0 Yes smoSAS.Scer\UAS.T:Zzzz\FLAG 0 Yes smoSAS.tub.Scer\UAS 0 Yes smoScer\UAS.attB.T:Hsap\MYC 0 Yes smoScer\UAS.cCa.T:Hsap\MYC 0 Yes smoScer\UAS.cCa 0 Yes smoScer\UAS.cDa 0 Yes smoScer\UAS.cFa 0 Yes smoScer\UAS.cHa.T:Hsap\MYC 0 Yes smoScer\UAS.cIa 0 Yes smoScer\UAS.cMa 0 Yes smoScer\UAS.cvdHa 0 Yes smoScer\UAS.cZa 0 Yes smoScer\UAS.J.T:Avic\GFP 0 Yes smoScer\UAS.J.T:Zzzz\FLAG 0 Yes smoScer\UAS.T:Avic\GFP,T:Hsap\LFA3-GPI 0 Yes smoScer\UAS.T:Avic\GFP,T:Hsap\Myr2 0 Yes smoScer\UAS.T:Avic\GFP-CFP 0 Yes smoScer\UAS.T:Avic\GFP-EGFP 0 Yes smoScer\UAS.T:Avic\GFP-YFP 0 Yes smoScer\UAS.T:Avic\GFP 0 Yes smoScer\UAS.T:Hsap\MYC,T:Avic\GFP-CFP 0 Yes smoScer\UAS.T:Hsap\MYC,T:Uuuu\Ub 0 Yes smoScer\UAS.T:Hsap\MYC 0 Yes smoScer\UAS.T:Ivir\HA1 0 Yes smoScer\UAS.T:SS-boss,T:Arus\HRP 0 Yes smoScer\UAS.T:Uuuu\Myr5 0 Yes smoScer\UAS.T:Zzzz\FLAG 0 Yes smoScer\UAS.T:Zzzz\TAP 0 Yes smoSD1.Scer\UAS.T:Zzzz\FLAG 0 Yes smoSD12.Scer\UAS.T:Zzzz\FLAG 0 Yes smoSD123.Scer\UAS.T:Avic\GFP-CFP 0 Yes smoSD123.Scer\UAS.T:Zzzz\FLAG 0 Yes smoSD123CK2SD.Scer\UAS.T:Hsap\MYC 0 Yes smoSDD.Scer\UAS.T:Hsap\MYC 0 Yes smoSDS.Scer\UAS.T:Hsap\MYC 0 Yes smoSMOEDS.Scer\UAS 0 Yes smoSSA.Scer\UAS.T:Hsap\MYC 0 Yes smoSSA.tub.Scer\UAS 0 Yes smoSSD.Scer\UAS.T:Hsap\MYC 0 Yes smoT1.Scer\UAS.T:Hsap\MYC 0 Yes smoT610A.T612A.Act5C 0 Yes smoT629A.Act5C 0 Yes smotTW116 0 Yes smotub.Scer\UAS.T:Hsap\MYC 0 Yes smotub.Scer\UAS.T:Zzzz\FLAG 0 Yes smoV.Ala.Act5C.T:Hsap\MYC 0 Yes smoV.Glu+740.Ala.Act5C.T:Hsap\MYC 0 Yes smoV.Glu+740.Glu.Act5C.T:Hsap\MYC 0 Yes smoV.Glu.Act5C.T:Hsap\MYC 0 Yes smoV.VI.Glu.Act5C.T:Hsap\MYC 0 Yes smoV.VI.VIII,Glu.Act5C.T:Hsap\MYC 0 Yes smoVI.Ala.Act5C.T:Hsap\MYC 0 Yes smoVI.Glu.Act5C.T:Hsap\MYC 0 Yes smoVIAΔC.Act5C 0 Yes smoVII.Ala.Act5C.T:Hsap\MYC 0 Yes smoVII.Glu.Act5C.T:Hsap\MYC 0 Yes smoVIII.Ala.Act5C.T:Hsap\MYC 0 Yes smoVIII.Glu.Act5C.T:Hsap\MYC 0 Yes smoVK5.CK2SA.Scer\UAS.T:Hsap\MYC 0 Yes smoW553L.Scer\UAS.T:Zzzz\FLAG 0 Yes smoW563A.Act5C 0 Yes smoWT.Scer\FRT.Avic\GFP.y.Scer\UAS.T:Ivir\HA1 0 Yes smoWT.Scer\UAS.T:Ivir\HA1 0 Yes smoWTPΔC.Act5C 0 Yes smoαTub84B.T:Ivir\HA1 0 Yes smoΔ626-678.Scer\UAS.T:Hsap\MYC 0 Yes smoΔ661-818.Scer\UAS.T:Avic\GFP-CFP 0 Yes smoΔC0.Scer\UAS.T:Zzzz\FLAG 0 Yes smoΔC1.Scer\UAS.T:Zzzz\FLAG 0 Yes smoΔC2.Scer\UAS.T:Zzzz\FLAG 0 Yes smoΔC3.Scer\UAS.T:Zzzz\FLAG 0 Yes smoΔC4.Scer\UAS.T:Zzzz\FLAG 0 Yes smoΔC730.Scer\UAS.T:Avic\GFP 0 Yes smoΔC818.Scer\UAS.T:Avic\GFP 0 Yes smoΔCT.Scer\UAS.T:Avic\GFP 0 Yes smoΔCT.Scer\UAS.T:Hsap\MYC 0 Yes smoΔFU.Act5C.T:Avic\GFP-EGFP 0 Yes smoΔFU.Scer\UAS 0 Yes smoΔN.Scer\UAS.T:Zzzz\FLAG 0 Yes
Disrupted in Not disrupted in Transgenic Constructs UAS construct characterization construct Insertions insertion of mobile activating element
Molecular Function inferred from physical interaction with
cos Biological Process inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with
ptc inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
Cellular Component ( 0 terms) Molecular Function inferred from sequence or structural similarity
non-traceable author statement
traceable author statement
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000139394
(assigned by RefGenome)
inferred from sequence or structural similarity
inferred from biological aspect of ancestor with PANTHER:PTN000139394
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139394
(assigned by RefGenome)
Biological Process traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139394
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139394
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from sequence or structural similarity
non-traceable author statement
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
traceable author statement
traceable author statement
Cellular Component inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139394
(assigned by RefGenome)
traceable author statement
inferred from sequence or structural similarity
non-traceable author statement
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000139395
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000139394
(assigned by RefGenome)
non-traceable author statement
traceable author statement
protein-protein fluorescent resonance energy transfer, pull down, anti tag western blot, autoradiography
pull down, anti tag western blot, anti tag coimmunoprecipitation
fluorescent resonance energy transfer, anti bait coimmunoprecipitation, anti tag western blot
anti tag coimmunoprecipitation, anti tag western blot, western blot
Interacts with Please look at the allele data
for full details of the genetic interactions
Linkouts
- A database of protein and genetic interactions
- A comprehensive database of gene and protein interactions.
Protein-protein interactions (PPI) from both known and predicted PPI data sets.
- A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.
OrthoDB Ortholog Groups
OrthoDB orthology group searches
Drosophila inclusive ortholog search
Dipteran inclusive ortholog search
Insect inclusive ortholog search
Arthropod inclusive ortholog search
Metazoa inclusive ortholog search
EOG6TTH36 )
Drosophila melanogaster
fruit fly
Drosophila pseudoobscura pseudoobscura
Y
EOG63R3VN )
Aedes aegypti
Yellow fever mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
EOG6QVB6V )
Apis mellifera
Western honey bee
Amel\GB53676
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG002505
Acromyrmex echinatior
Panamanian leafcutter ant
Aech\AECH16131
Atta cephalotes
Leafcutter ant
Acep\ACEP18377
Camponotus floridanus
Florida carpenter ant
Cflo\CFLO17965
Harpegnathos saltator
Jerdons jumping ant
Hsal\HSAL21429
Linepithema humile
Argentine ant
Lhum\LH10183
Pogonomyrmex barbatus
Red harvester ant
Pbar\PB27329
Solenopsis invicta
Red fire ant
Sinv\SINV18048
Acyrthosiphon pisum
Pea aphid
Pediculus humanus
Human body louse
Tribolium castaneum
Red flour beetle
EOG62547M )
Ixodes scapularis
Deer tick
EOG60GRHQ )
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Xenopus tropicalis
Western clawed frog
Gallus gallus
Domestic chicken
Rattus norvegicus
Norway rat
AAA Orthologs (11)
based on analysis using Dmel annotation version 4.3
Drosophila pseudoobscura pseudoobscura
Bloomington Kyoto VDRC Please Note FlyBase no
longer curates genomic clone accessions so this list
may not be complete
Please Note This section lists
cDNAs and ESTs that fall within the genomic extent
of the gene model, which may include cDNAs and ESTs
of genes within introns, or of overlapping genes.
Please see
GBrowse for alignment of the cDNAs and ESTs
to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones Other clones cDNA Clones, End Sequenced
(ESTs) BDGP DGC clones Other clones Linkouts
- Results from RNAi screens.
- GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
Source for database identity of Source for database merge of Additional comments A region in the C-terminus of
smo protein is sufficient for its interaction with
fu protein (spans amino acids 985 to 1036).
Phosphorylation activates
smo protein by inducing a conformational switch. This occurs by antagonising multiple Arg clusters in the
smo cytoplasmic tail. The Arg clusters inhibit
smo protein by blocking its cell surface expression and keeping it in an inactive conformation that is maintained by electrostatic interactions. Phosphorylation disrupts the interactions and induces a conformational switch and dimerisation of
smo protein cytoplasmic tails, which is essential for pathway activation.
Identified as a component of the
hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a
hh signaling reporter construct in Clone 8 cells.
smo protein accumulates in the plasma membrane of cells that lack
ptc activity, whereas when co-expressed with
ptc ,
smo protein localises exclusively intracellularly, in distinct punctate structures.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
dsRNA made from templates generated with primers against this gene tested in a cl-8 cell reporter assay to examine any role of
smo in the
hh signalling pathway.
The last 301 amino acids of the cos COOH terminus are sufficient to provide full association with smo.
Epistatic analysis places cos function downstream of ptc and smo.
dsRNA corresponding to smo used in S2 cultured cell assay.
17 alleles of
smo have been recovered in a screen for mutations with
smo -like mutant phenotypes in clones in the wing.
Control of
smo localisation appears to be a crucial step in
hh signalling.
ptc -
smo signalling has a role in head morphogenesis to promote cell proliferation via activation of
babo .
ptc protein destabilises
smo protein in the absence of
hh protein.
The
hh signalling components
smo and
ci are required in cells posterior to
hh to maintain
ptc expression, whereas
fu is not necessary in these cells.
ptc protein normally binds
hh gene product without any help of the
smo gene product, though
smo is also a part of the receptor complex that binds
hh and transduces the
hh signal. The mechanism of signal transduction may involve
hh binding specifically to
ptc and inducing a conformational change leading to the release of latent
smo activity.
Anterior cells in the wing that lack
smo function no longer obey a lineage restriction in the normal position of the anterior-posterior boundary.
Complementation and epistasis tests indicate that
oro is not allelic to
smo .
smo encodes a serpentine protein highly conserved in evolution.
smo activity is required for transduction of
hh but not
wg .
smo acts downstream from
ptc to transduce the
hh signal.
Loss of
smo function causes a
hh -like phenotype.
Morphogenetic furrow progression is significantly delayed, but not prevented, in
smo clones.
smo is a putative receptor of the
hh signal.
smo encodes a seven-pass membrane protein. In direction of increasing cytology: anon-21Ca+ anon-21Cb- smo+ anon-21Cc-
smo activity is required in wing anterior cells along the A/P boundary for these cells both to transduce
hh and to limit its further movement into the anterior compartment.
ptc regulates
smo activity in response to
hh signalling.
In competition binding, cross-linking and co-immunoprecipitation experiments no binding of tagged
hh protein to
smo protein or its rat homolog could be detected, although
hh protein can bind to the protein encoded by the mouse homolog of
ptc .
Segment polarity gene
smo is required for the response of cells to
hh signalling during the development of both the embryonic segments and imaginal discs. Structure of the
smo protein suggests it may act as a receptor for the
hh ligand.
The effects of removing
smo gene activity from embryos in which
hh or
wg is ectopically expressed suggest a role for
smo in the
hh signalling pathway.
All alleles are zygotic lethal, cold sensitive, at the lower temperature the phenotype of all alleles is quite variable suggesting that the gene may be maternally expressed.
Direct
wg autoregulation differs from
wg signalling to adjacent cells in the importance of
fu ,
smo and
ci relative to
sgg and
arm .
The
smo gene acts downstream of
wg in pattern formation.
Mutations in zygotic polarity gene
smo do not interact with
RpII140wimp .
Sequence Crossreferences
- A searchable database of RefSeq genes.
Other Crossreferences
domains - A database of protein families, domains, and functional sites
- Patterns of gene expression in Drosophila embryogenesis
Linkouts
- A database of protein and genetic interactions
- A comprehensive database of gene and protein interactions.
- Results from RNAi screens.
- Cell culture data for RNAi and other high-throughput technologies
- Adult expression by tissue, using Affymetrix Dros2 array
- Integrated genomics database for Drosophila, Anopheles, and C.elegans
- A curated knowledgebase of Drosophila melanogaster pathways
- GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
- A cyberspace guide to Drosophila development and metazoan evolution
Protein-protein interactions (PPI) from both known and predicted PPI data sets.
- Data generated by the modENCODE project.
- A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.
Reported As Symbol Synonym smo
(
Casso et al., 2008 ,
Friggi-Grelin et al., 2008 ,
Ishii, 2005 ,
Briscoe and Therond, 2005 ,
Lu et al., 2006 ,
Zhou et al., 2006 ,
Chen et al., 2007 ,
Casso et al., 2007 ,
Smelkinson and Kalderon, 2006 ,
Vrailas et al., 2006 ,
Croker et al., 2006 ,
Umetsu et al., 2006 ,
Wei et al., 2005 ,
Molnar and de Celis, 2006 ,
Kent et al., 2006 ,
Yao et al., 2006 ,
McLellan et al., 2006 ,
Merianda et al., 2005 ,
Cook, 2007.7.24 ,
Jia et al., 2005 ,
Firth and Baker, 2005 ,
D'Costa et al., 2006 ,
Colosimo and Tolwinski, 2006 ,
Carroll et al., 2012 ,
Kirilly et al., 2005 ,
Cheng et al., 2010 ,
Raabe et al., 2004 ,
Nybakken et al., 2005 ,
Deshpande and Schedl, 2005 ,
Molnar et al., 2011 ,
Terriente Felix and de Celis, 2008.1.16 ,
Vrailas and Moses, 2006 ,
Lecuyer et al., 2007 ,
Varjosalo et al., 2006 ,
Torroja et al., 2005 ,
Firth and Baker, 2005 ,
Ambrus et al., 2007 ,
Wang and Price, 2008 ,
Zhou and Kalderon, 2011 ,
Deshpande et al., 2009 ,
Chen et al., 2008 ,
Hartman et al., 2010 ,
Marks and Kalderon, 2011 ,
Cheng et al., 2012 ,
Su et al., 2011 ,
Toku et al., 2011 ,
Smelkinson et al., 2007 ,
Goodfellow et al., 2007 ,
Escudero et al., 2007 ,
Corrigall et al., 2007 ,
Fan and Bergmann, 2008 ,
Gallet et al., 2008 ,
Ruel et al., 2007 ,
Tanaka-Matakatsu and Du, 2008 ,
Claret et al., 2007 ,
Molnar et al., 2007 ,
Sweeney et al., 2007 ,
Bashaw, 2007 ,
Farzan et al., 2008 ,
Firth and Baker, 2009 ,
Melicharek et al., 2008 ,
Cabernard and Affolter, 2005 ,
Ogden et al., 2008 ,
Jia et al., 2009 ,
Escudero and Freeman, 2007 ,
Foronda et al., 2009 ,
Xie et al., 2005 ,
Punzo et al., 2004 ,
Blanco et al., 2009 ,
Renault et al., 2009 ,
Vied and Kalderon, 2009 ,
Bejarano et al., 2007 ,
Lim et al., 2008 ,
Orme and Leevers, 2005 ,
Khaliullina et al., 2009 ,
Baker et al., 2009 ,
Mosimann et al., 2009 ,
Sisson et al., 2006 ,
Zhang et al., 2005 ,
Gazi et al., 2009 ,
Schlichting and Dahmann, 2008 ,
Baonza and Freeman, 2005 ,
Landsberg et al., 2009 ,
Firth et al., 2010 ,
Lopes and Casares, 2010 ,
Li et al., 2010 ,
de Celis and Molnar, 2010 ,
Terriente-Félix et al., 2010 ,
Raisin et al., 2010 ,
Yavari et al., 2010 ,
Pospisilik et al., 2010 ,
Nicholson et al., 2011 ,
Beltran et al., 2007 ,
Suh et al., 2006 ,
Baron et al., 2009 ,
Terriente-Félix et al., 2011 ,
Wasbrough et al., 2010 ,
Schilling et al., 2011 ,
Chou et al., 2010 ,
Zhou and Kalderon, 2010 ,
Wu et al., 2012 ,
Jia et al., 2010 ,
Christiansen et al., 2012 ,
Camp et al., 2010 ,
Krzemien et al., 2012 ,
Bhattacharya and Baker, 2011 ,
Japanese National Institute of Genetics, 2012.5.21 ,
Wang et al., 2012 ,
Zhang et al., 2011 )
Smo
(
Osterlund and Kogerman, 2006 ,
Kuwabara and Labouesse, 2002 ,
Chen et al., 1999 ,
Fisher and Howie, 2006 ,
Beenken and Mohammadi, 2006 ,
Legent et al., 2012 ,
Molnar et al., 2007 ,
Ogden et al., 2006 ,
Xia et al., 2012 ,
Wilson and Chuang, 2006 ,
Liu et al., 2008 ,
Zhao and Jiang, 2008 ,
Su et al., 2008 ,
Zhao et al., 2007 ,
Liu et al., 2007 ,
Jekely and Rorth, 2003 ,
Wu and Mlodzik, 2008 ,
Walthall et al., 2007 ,
Dawber et al., 2005 ,
Shi et al., 2011 ,
Ayers et al., 2009 ,
González et al., 2008 ,
Mukai et al., 2010 ,
Mao and Freeman, 2009 ,
Farzan et al., 2009 ,
Jia et al., 2010 ,
Cheng et al., 2012 ,
Zheng et al., 2010 ,
Fan et al., 2012 ,
Zhang et al., 2011 )
SMO
(
Sanial and Plessis, 2007 ,
Plessis et al., 2007 ,
Zhao et al., 2007 ,
Stoll et al., 2010 ,
Malpel et al., 2007 ,
Subramanian and Gadgil, 2010 )
Name Synonym smoothened
(
Hooper, 1997.1.29 ,
Jones et al., 2006 ,
Yao et al., 2006 ,
Merianda et al., 2005 ,
Cheng et al., 2010 ,
Sweeney et al., 2007 ,
Casso et al., 2008 ,
Vrailas and Moses, 2006 ,
Zhao et al., 2007 ,
Hime et al., 2004 ,
Escudero et al., 2007 ,
Corrigall et al., 2007 ,
Fan and Bergmann, 2008 ,
Ogden et al., 2008 ,
Foronda et al., 2009 ,
Orme and Leevers, 2005 ,
Lim et al., 2008 ,
Baonza and Freeman, 2005 ,
Landsberg et al., 2009 ,
Lopes and Casares, 2010 ,
de Celis and Molnar, 2010 ,
Terriente-Félix et al., 2010 ,
Nicholson et al., 2011 ,
Baron et al., 2009 ,
Krzemien et al., 2012 ,
Bhattacharya and Baker, 2011 ,
Marks and Kalderon, 2011 )
Smoothened
(
Sanial and Plessis, 2007 ,
Casso et al., 2007 ,
Plessis et al., 2007 ,
Panakova and Eaton, 2006 ,
Maitra et al., 2006 ,
Beenken and Mohammadi, 2006 ,
Molnar et al., 2007 ,
Bangi et al., 2007 ,
Ogden et al., 2006 ,
Colosimo and Tolwinski, 2006 ,
Molnar et al., 2011 ,
Wilson and Chuang, 2006 ,
Liu et al., 2008 ,
Su et al., 2008 ,
Torroja et al., 2005 ,
Zhao et al., 2007 ,
Malpel et al., 2007 ,
Wu and Mlodzik, 2008 ,
Claret et al., 2007 ,
Farzan et al., 2008 ,
Walthall et al., 2007 ,
Jia et al., 2009 ,
Dawber et al., 2005 ,
Escudero and Freeman, 2007 ,
Shi et al., 2011 ,
Blanco et al., 2009 ,
Ayers et al., 2009 ,
Bejarano et al., 2007 ,
Khaliullina et al., 2009 ,
Mosimann et al., 2009 ,
Gazi et al., 2009 ,
Mukai et al., 2010 ,
Yavari et al., 2010 ,
Farzan et al., 2009 ,
Schilling et al., 2011 ,
Chou et al., 2010 ,
Zhou and Kalderon, 2010 ,
Jia et al., 2010 ,
Camp et al., 2010 ,
Cheng et al., 2012 ,
Fan et al., 2012 )
Secondary FlyBase
IDs Generate a list of List References by type
Hedgehog is required for CySC self-renewal but does not contribute to the GSC niche in the Drosophila testis. [FBrf0220178] Developmental Regulation of Nucleolus Size during Drosophila Eye Differentiation. [FBrf0220989] Differential activity of Drosophila hox genes induces Myosin expression and can maintain compartment boundaries. [FBrf0220928] The extracellular loops of Smoothened play a regulatory role in control of Hedgehog pathway activation. [FBrf0217120] Drosophila G-protein-coupled receptor kinase 2 regulates cAMP-dependent Hedgehog signaling. [FBrf0216860] Ligand-independent activation of the Hedgehog pathway displays non-cell autonomous proliferation during eye development in Drosophila. [FBrf0219046] Hh-induced Smoothened conformational switch is mediated by differential phosphorylation at its C-terminal tail in a dose- and position-dependent manner. [FBrf0218374] The muscle pattern of the Drosophila abdomen depends on a subdivision of the anterior compartment of each segment. [FBrf0216853] A screen for x-linked mutations affecting Drosophila photoreceptor differentiation identifies casein kinase 1α as an essential negative regulator of wingless signaling. [FBrf0217484] Hh signalling is essential for somatic stem cell maintenance in the Drosophila testis niche. [FBrf0218815] Distinct phosphorylations on Kinesin costal-2 mediate differential hedgehog signaling strength. [FBrf0217531] Cytoneme-mediated delivery of hedgehog regulates the expression of bone morphogenetic proteins to maintain germline stem cells in Drosophila. [FBrf0218031] Genetic screen for regulators of lymph gland homeostasis and hemocyte maturation in Drosophila. [FBrf0217733] Regulation of stem cells by intersecting gradients of long-range niche signals. [FBrf0219709] Drosophila follicle stem cells are regulated by proliferation and niche adhesion as well as mitochondria and ROS. [FBrf0217898] Drosophila miR-5 suppresses Hedgehog signaling by directly targeting Smoothened. [FBrf0219894] USP8 promotes smoothened signaling by preventing its ubiquitination and changing its subcellular localization. [FBrf0218247] Hedgehog signaling regulates nociceptive sensitization. [FBrf0216292] A Network of Broadly Expressed HLH Genes Regulates Tissue-Specific Cell Fates. [FBrf0216641] A Novel Interaction Between hedgehog and Notch Promotes Proliferation at the Anterior-Posterior Organizer of the Drosophila Wing. [FBrf0212986] Regulation of mammalian Gli proteins by Costal 2 and PKA in Drosophila reveals Hedgehog pathway conservation. [FBrf0213757] Role of the Drosophila non-visual ß-arrestin kurtz in hedgehog signalling. [FBrf0213301] Notch-dependent expression of the archipelago ubiquitin ligase subunit in the Drosophila eye. [FBrf0212669] Cell-sorting at the a/p boundary in the Drosophila wing primordium: a computational model to consolidate observed non-local effects of hh signaling. [FBrf0213439] The Hedgehog-induced Smoothened conformational switch assembles a signaling complex that activates Fused by promoting its dimerization and phosphorylation. [FBrf0216910] Sequential phosphorylation of smoothened transduces graded hedgehog signaling. [FBrf0216577] A conserved function of the chromatin ATPase Kismet in the regulation of hedgehog expression. [FBrf0212899] Reconstruction and crosstalk of protein-protein interaction networks of Wnt and Hedgehog signaling in Drosophila melanogaster. [FBrf0216393] Transduction of the Hedgehog signal through the dimerization of Fused and the nuclear translocation of Cubitus interruptus. [FBrf0217260] Hedgehog activates fused through phosphorylation to elicit a full spectrum of pathway responses. [FBrf0214655]
All reviews listed in FlyBase were published before 2011 
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