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Gene Dmel\spir

General Information
Symbol	Dmel\spir	Species	D. melanogaster
Name	spire	Annotation symbol	CG10076
Feature type	protein_coding_gene	FlyBase ID	FBgn0003475
Gene Model Status	Current	Stock availability	25 publicly available
Also Known As	p150-Spir, Spire, 38C.37, 38C.34
Genomic Location
Chromosome (arm)	2L	Recombination map	2-54
Cytogenetic map	38C5-38C5	Sequence location	2L:20,311,219..20,348,386 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene spire is referred to in FlyBase by the symbol Dmel\spir (CG10076, FBgn0003475). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; microtubule binding. There is experimental evidence that it is involved in the biological process: chorion-containing eggshell formation; actin filament-based process; actin nucleation; regulation of cytoskeleton organization; pole plasm assembly; actin filament organization; pole plasm RNA localization; karyosome formation. 56 alleles are reported. The phenotypes of these alleles are annotated with: karyosome; egg chamber; oocyte nucleus. It has 10 annotated transcripts and 10 annotated polypeptides. Protein features are: KIND; WH2 domain; Zinc finger, FYVE/PHD-type; Zinc finger, RING/FYVE/PHD-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, larval/adult midgut, adult hindgut, larval Malpighian tubules, adult ovary. Gene sequence location is 2L:20311219..20348386.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	spir: spire (T. Schupbach) Maternal-effect lethal; homozygous females lay eggs which sometimes (5-10%) have a "peak" (spire) of dorsal appendage material sitting over the anterior end of the egg, instead of two distinct dorsal appendages. Such eggs are similar to eggs formed by the female-sterile mutation fs(1)K10, but the extent of dorsal appendage material on spir eggs is much more variable than that of fs(1)K10 eggs. Mutant females produce embryos lacking polar granules, pole cells, and normal abdominal segmentation. In combination with Bic-D, however, abdominal segmentation does develop in the anterior half of the embryo; improper localization of abdominal determinants also indicated by the lack of posterior localization of vasa protein. Cellularization of the blastoderm irregularly defective with nuclei of different sizes and densities. Resemble embryos formed by other grandchildless-knirps-like mutations, such as vasa or tudor, but in addition, some of the embryos from spire females appear also to be dorsalized.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Alleles spir[MI05646]
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 38C5-38C5   Limits computationally determined from genome sequence between P{lacW}k09005 and P{EP}CG16798EP401&P{lacW}k07219
Experimentally Determined Cytological Location
Cytogenetic map Notes References 38C-38C     (Kerkhoff, 1999.5.20)
Experimentally Determined Recombination Data
Location	2-54   2-54.5 (Manseau and Schupbach, 1989)
Left of (cM)
Right of (cM)
Notes	Mapped using spir1. (Manseau and Schupbach, 1989)
Gene Model & Products
Please see the GBrowse view of Dmel\spir for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.49 Low-frequency RNA-Seq exon junction(s) not annotated. Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters. Gene model includes transcripts encoding non-overlapping portions of the full CDS.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) spir-RA FBtr0081352  NM_165323   4881   1020 spir-RB FBtr0081353  NM_080115   4794   991 spir-RC FBtr0081355  NM_165325   3437   625 spir-RD FBtr0081354  NM_165324   2666   585 spir-RE FBtr0301884  NM_001169549   4791   990 spir-RF FBtr0306556  NM_001259172   4875   1018 spir-RG FBtr0307893  NM_001259173   2669   586 spir-RH FBtr0307894  NM_001259174   2182   488 spir-RI FBtr0339233     2212   498 spir-RJ FBtr0339234     5598   586
Additional Transcript Data & Comments
Reported size (kB)	4.5, 2.7 (northern blot) (Wellington et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein spir-PA   FBpp0080884   114.9   1020   6.91     NP_724254   AAF53884 spir-PB   FBpp0080885   111.5   991   7.09     NP_524854   AAN11070 spir-PC   FBpp0080887   69.4   625   9.36     NP_724256   AAN11072 spir-PD   FBpp0080886   66.4   585   6.17     NP_724255   AAN11071 spir-PE   FBpp0291098   111.3   990   7.14     NP_001163020   ACZ94306 spir-PF   FBpp0297511   114.7   1018   6.96     NP_001246101   AFH03775 spir-PG   FBpp0300327   66.5   586   6.12     NP_001246102   AFH03776 spir-PH   FBpp0300328   55.0   488   5.92     NP_001246103   AFH03777 spir-PI   FBpp0308340   56.3   498   5.98       spir-PJ   FBpp0308341   66.5   586   6.12
Additional Polypeptide Data & Comments
Reported size (kDa)	990, 585 (aa) (Wellington et al., 1999)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • WH2 domain (IPR003124) KIND (IPR011019) Zinc finger, FYVE/PHD-type (IPR011011) Zinc finger, RING/FYVE/PHD-type (IPR013083)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA390587   AA695386   AA803260   AE014134 AAF53884   AE014134 AAN11070   AE014134 AAN11071   AE014134 AAN11072   AC002503   AC004735   AE014134 ACZ94306   AF184975 AAF23615   AF184976 AAF23616   AE014134 AFH03775   AE014134 AFH03776   AE014134 AFH03777   AI062240   AI108408   AI113886   AI114049   AI135528   AI386773   AI513246   AI518150   AI543415   AI543996   AI945463   AI945583   AJ238876 CAB62901   AW944620   AX093986   AY075586 AAL68390   AY089503 AAL90241   BE976552   BE977094   BF486592   BF486787   BF486863   BF488843   BF491195   BF493022   BF493023   BF493024   BF493025   BF494072   BF495639   BF500986   BF501119   BF501528   BF501879   BF501989   BF503635   BF503781   BF506040   BG634198   BG638840   BH610047   BI164144   BI168862   BI169590   BI214302   BI229205   BI234083   BI236612   BI352574   BI369829   BI370402   BI370880   BI565006   BI566165   BI567889   BI576695   BI604449   BI609196   BI609619   BI626240   BI626454   BI628860   BI629666   BI634527   BI638686   BI641678   BT016094   BT044175   BT122081   BT124773   BT124774   BT125974   CK658982   CK661336   CO179368   CO189705   CO196636   CO263482   CO265015   CO267711   CO272398   CO272433   CO281029   CO281703   CO282280   CO282382   CO282977   CO295298   CO298178   CO300107   CO301503   CO301865   CO316915   CO320967   CO323162   CO329223   CO330469   CO333771   CZ472921   CZ472922   CZ482545   CZ486220   CZ490456   CZ490457   EC062073   EC064191   EC079448   EC083047   EC085232   EC089468   EC090151   EC090326   EC194587   EC197634   EC197673   EC199537   EC200071   EC200197   EC200715   EC201343   EC205817   EC210602   EC211607   EC217930   EC219666   EC221680   EC228795   EC229850   EC230323   EC233642   EC240182   EC245088   EC245636   EC250307   EC250642   EC251872   ES692248
UniProtKB/Swiss-Prot	Q9U1K1
UniProtKB/TrEMBL	E1JHM3
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   pole plasm   (Fisher et al., 2012) Comment:maternally deposited organism   • pole plasm (Fisher et al., 2012) Comment:maternally deposited embryonic stage 1 -- 4   pole plasm | posterior   (Lecuyer et al., 2007) embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) pole cell   (Fisher et al., 2012) embryonic stage 5   pole cell   (Lecuyer et al., 2007) embryonic stage 7 -- 8   pole cell   (Fisher et al., 2012) embryonic stage 9 -- 10   germline cell   (Fisher et al., 2012) embryonic stage 11 -- 12   clypeo-labral primordium   (Fisher et al., 2012) foregut primordium   (Fisher et al., 2012) germline cell   (Fisher et al., 2012) gnathal primordium   (Fisher et al., 2012) hindgut proper primordium   (Fisher et al., 2012) labral sensory complex primordium   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) rectum primordium   (Fisher et al., 2012) Comment:reported as rectum specific anlage ventral epidermis primordium   (Fisher et al., 2012) ventral imaginal precursor   (Fisher et al., 2012) Comment:reported as ventral imaginal precursor specific anlage embryonic stage 13 -- 16   atrium   (Fisher et al., 2012) embryonic epipharynx   (Fisher et al., 2012) embryonic foregut   (Fisher et al., 2012) embryonic foregut sensory structure   (Fisher et al., 2012) embryonic head sensory system   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic hypopharynx   (Fisher et al., 2012) embryonic midgut chamber   (Fisher et al., 2012) embryonic proventriculus   (Fisher et al., 2012) embryonic rectum   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) germline cell   (Fisher et al., 2012) gonad   (Fisher et al., 2012) sensory nervous system primordium   (Fisher et al., 2012) ventral imaginal precursor   (Fisher et al., 2012)
Additional Descriptive Data
	spir expression at embryonic stages 1-4 is localized to the posterior pole of the embryo. (Lecuyer et al., 2007) Both spir transcripts are present in nurse cells and follicle cells from germarium region 2 onwards. (Wellington et al., 1999)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cell cortex inferred from direct assay (Rosales-Nieves et al., 2006) cytoplasm inferred from direct assay (Rosales-Nieves et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, larval/adult midgut, adult hindgut, larval Malpighian tubules, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    43 central nervous system, larvae L3    10 central nervous system, pupae P8    18 head, virgin 1-day female    32 head, virgin 4-day female    29 head, virgin 20-day female    24 head, mated 1-day female    25 head, mated 4-day female    22 head, mated 20-day female    21 head, mated 1-day male    51 head, mated 4-day male    40 head, mated 20-day male    35 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    21 digestive system, 1-day adult    14 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    25 carcass, larvae L3 wandering    5 carcass, 1-day adult    6 carcass, 4-day adult    8 carcass, 20-day adult    9 ovary, virgin 4-day female    50 ovary, mated 4-day female    49 testis, mated 4-day male    84 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (43) central nervous system, larvae L3    10 central nervous system, pupae P8    18 head, virgin 1-day female  (32) head, virgin 4-day female  (29) head, virgin 20-day female    24 head, mated 1-day female    25 head, mated 4-day female    22 head, mated 20-day female    21 head, mated 1-day male  (51) head, mated 4-day male  (40) head, mated 20-day male  (35) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    21 digestive system, 1-day adult    14 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    25 carcass, larvae L3 wandering    5 carcass, 1-day adult    6 carcass, 4-day adult    8 carcass, 20-day adult    9 ovary, virgin 4-day female  (50) ovary, mated 4-day female  (49) testis, mated 4-day male  (84) accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    43 central nervous system, larvae L3    10 central nervous system, pupae P8    18 head, virgin 1-day female    32 head, virgin 4-day female    29 head, virgin 20-day female    24 head, mated 1-day female    25 head, mated 4-day female    22 head, mated 20-day female    21 head, mated 1-day male    51 head, mated 4-day male    40 head, mated 20-day male    35 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    21 digestive system, 1-day adult    14 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    25 carcass, larvae L3 wandering    5 carcass, 1-day adult    6 carcass, 4-day adult    8 carcass, 20-day adult    9 ovary, virgin 4-day female    50 ovary, mated 4-day female    49 testis, mated 4-day male    84 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    43 central nervous system, larvae L3    10 central nervous system, pupae P8    18 head, virgin 1-day female    32 head, virgin 4-day female    29 head, virgin 20-day female    24 head, mated 1-day female    25 head, mated 4-day female    22 head, mated 20-day female    21 head, mated 1-day male    51 head, mated 4-day male    40 head, mated 20-day male    35 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    21 digestive system, 1-day adult    14 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    25 carcass, larvae L3 wandering    5 carcass, 1-day adult    6 carcass, 4-day adult    8 carcass, 20-day adult    9 ovary, virgin 4-day female    50 ovary, mated 4-day female    49 testis, mated 4-day male    84 accessory gland, mated 4-day male    13 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    43 central nervous system, larvae L3    10 central nervous system, pupae P8    18 head, virgin 1-day female    32 head, virgin 4-day female    29 head, virgin 20-day female    24 head, mated 1-day female    25 head, mated 4-day female    22 head, mated 20-day female    21 head, mated 1-day male    51 head, mated 4-day male    40 head, mated 20-day male    35 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    21 digestive system, 1-day adult    14 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    25 carcass, larvae L3 wandering    5 carcass, 1-day adult    6 carcass, 4-day adult    8 carcass, 20-day adult    9 ovary, virgin 4-day female    50 ovary, mated 4-day female    49 testis, mated 4-day male    84 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (43) central nervous system, larvae L3    10 central nervous system, pupae P8    18 head, virgin 1-day female  32 head, virgin 4-day female  29 head, virgin 20-day female    24 head, mated 1-day female    25 head, mated 4-day female    22 head, mated 20-day female    21 head, mated 1-day male  (51) head, mated 4-day male  (40) head, mated 20-day male  (35) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    21 digestive system, 1-day adult    14 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    25 carcass, larvae L3 wandering    5 carcass, 1-day adult    6 carcass, 4-day adult    8 carcass, 20-day adult    9 ovary, virgin 4-day female  (50) ovary, mated 4-day female  (49) testis, mated 4-day male  (84) accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    43 central nervous system, larvae L3    10 central nervous system, pupae P8    18 head, virgin 1-day female    32 head, virgin 4-day female    29 head, virgin 20-day female    24 head, mated 1-day female    25 head, mated 4-day female    22 head, mated 20-day female    21 head, mated 1-day male    51 head, mated 4-day male    40 head, mated 20-day male    35 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    21 digestive system, 1-day adult    14 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    25 carcass, larvae L3 wandering    5 carcass, 1-day adult    6 carcass, 4-day adult    8 carcass, 20-day adult    9 ovary, virgin 4-day female    50 ovary, mated 4-day female    49 testis, mated 4-day male    84 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    43 central nervous system, larvae L3    10 central nervous system, pupae P8    18 head, virgin 1-day female    32 head, virgin 4-day female    29 head, virgin 20-day female    24 head, mated 1-day female    25 head, mated 4-day female    22 head, mated 20-day female    21 head, mated 1-day male    51 head, mated 4-day male    40 head, mated 20-day male    35 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    21 digestive system, 1-day adult    14 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    25 carcass, larvae L3 wandering    5 carcass, 1-day adult    6 carcass, 4-day adult    8 carcass, 20-day adult    9 ovary, virgin 4-day female    50 ovary, mated 4-day female    49 testis, mated 4-day male    84 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the pupal period, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    7 embryo 04-06hr    5 embryo 06-08hr    5 embryo 08-10hr    7 embryo 10-12hr    9 embryo 12-14hr    13 embryo 14-16hr    23 embryo 16-18hr    26 embryo 18-20hr    20 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP    38 pupae 3d postWPP    22 pupae 4d postWPP    25 adult male 01day    30 adult male 05day    39 adult male 30day    40 adult female 01day    13 adult female 05day    13 adult female 30day    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    7 embryo 04-06hr    5 embryo 06-08hr    5 embryo 08-10hr    7 embryo 10-12hr    9 embryo 12-14hr    13 embryo 14-16hr    23 embryo 16-18hr  26 embryo 18-20hr    20 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP  (38) pupae 3d postWPP    22 pupae 4d postWPP    25 adult male 01day  (30) adult male 05day  (39) adult male 30day  (40) adult female 01day    13 adult female 05day    13 adult female 30day    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    7 embryo 04-06hr    5 embryo 06-08hr    5 embryo 08-10hr    7 embryo 10-12hr    9 embryo 12-14hr    13 embryo 14-16hr    23 embryo 16-18hr    26 embryo 18-20hr    20 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP    38 pupae 3d postWPP    22 pupae 4d postWPP    25 adult male 01day    30 adult male 05day    39 adult male 30day    40 adult female 01day    13 adult female 05day    13 adult female 30day    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    7 embryo 04-06hr    5 embryo 06-08hr    5 embryo 08-10hr    7 embryo 10-12hr    9 embryo 12-14hr    13 embryo 14-16hr    23 embryo 16-18hr    26 embryo 18-20hr    20 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP    38 pupae 3d postWPP    22 pupae 4d postWPP    25 adult male 01day    30 adult male 05day    39 adult male 30day    40 adult female 01day    13 adult female 05day    13 adult female 30day    14 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    7 embryo 04-06hr    5 embryo 06-08hr    5 embryo 08-10hr    7 embryo 10-12hr    9 embryo 12-14hr    13 embryo 14-16hr    23 embryo 16-18hr    26 embryo 18-20hr    20 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP    38 pupae 3d postWPP    22 pupae 4d postWPP    25 adult male 01day    30 adult male 05day    39 adult male 30day    40 adult female 01day    13 adult female 05day    13 adult female 30day    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    7 embryo 04-06hr    5 embryo 06-08hr    5 embryo 08-10hr    7 embryo 10-12hr    9 embryo 12-14hr    13 embryo 14-16hr    23 embryo 16-18hr  26 embryo 18-20hr    20 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP  (38) pupae 3d postWPP    22 pupae 4d postWPP    25 adult male 01day  30 adult male 05day  (39) adult male 30day  (40) adult female 01day    13 adult female 05day    13 adult female 30day    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    7 embryo 04-06hr    5 embryo 06-08hr    5 embryo 08-10hr    7 embryo 10-12hr    9 embryo 12-14hr    13 embryo 14-16hr    23 embryo 16-18hr    26 embryo 18-20hr    20 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP    38 pupae 3d postWPP    22 pupae 4d postWPP    25 adult male 01day    30 adult male 05day    39 adult male 30day    40 adult female 01day    13 adult female 05day    13 adult female 30day    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    7 embryo 04-06hr    5 embryo 06-08hr    5 embryo 08-10hr    7 embryo 10-12hr    9 embryo 12-14hr    13 embryo 14-16hr    23 embryo 16-18hr    26 embryo 18-20hr    20 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP    38 pupae 3d postWPP    22 pupae 4d postWPP    25 adult male 01day    30 adult male 05day    39 adult male 30day    40 adult female 01day    13 adult female 05day    13 adult female 30day    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    3 embryonic 1182-4H    174 embryonic GM2    61 embryonic Kc167    17 embryonic S1    85 embryonic S3    9 leg disc CME L1    5 wing disc CME-W2    40 wing disc ML-DmD8    31 wing disc ML-DmD9    39 wing disc ML-DmD16-c3    49 wing disc ML-DmD21    1 wing disc ML-DmD32    37 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    98 antennal disc ML-DmD20-c5    40 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    57 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    3 embryonic 1182-4H  (174) embryonic GM2  (61) embryonic Kc167    17 embryonic S1  (85) embryonic S3    9 leg disc CME L1    5 wing disc CME-W2  (40) wing disc ML-DmD8  (31) wing disc ML-DmD9  (39) wing disc ML-DmD16-c3  (49) wing disc ML-DmD21    1 wing disc ML-DmD32  (37) haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11  (98) antennal disc ML-DmD20-c5  (40) mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2  (30) tumorous blood cells mbn2  (57) ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    3 embryonic 1182-4H  (174) embryonic GM2    61 embryonic Kc167    17 embryonic S1    85 embryonic S3    9 leg disc CME L1    5 wing disc CME-W2    40 wing disc ML-DmD8    31 wing disc ML-DmD9    39 wing disc ML-DmD16-c3    49 wing disc ML-DmD21    1 wing disc ML-DmD32    37 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    98 antennal disc ML-DmD20-c5    40 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    57 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    3 embryonic 1182-4H    174 embryonic GM2    61 embryonic Kc167    17 embryonic S1    85 embryonic S3    9 leg disc CME L1    5 wing disc CME-W2    40 wing disc ML-DmD8    31 wing disc ML-DmD9    39 wing disc ML-DmD16-c3    49 wing disc ML-DmD21    1 wing disc ML-DmD32    37 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    98 antennal disc ML-DmD20-c5    40 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    57 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    3 embryonic 1182-4H    174 embryonic GM2    61 embryonic Kc167    17 embryonic S1    85 embryonic S3    9 leg disc CME L1    5 wing disc CME-W2    40 wing disc ML-DmD8    31 wing disc ML-DmD9    39 wing disc ML-DmD16-c3    49 wing disc ML-DmD21    1 wing disc ML-DmD32    37 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    98 antennal disc ML-DmD20-c5    40 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    57 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    3 embryonic 1182-4H  (174) embryonic GM2  (61) embryonic Kc167    17 embryonic S1  (85) embryonic S3    9 leg disc CME L1    5 wing disc CME-W2  (40) wing disc ML-DmD8  31 wing disc ML-DmD9  (39) wing disc ML-DmD16-c3  (49) wing disc ML-DmD21    1 wing disc ML-DmD32  (37) haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11  (98) antennal disc ML-DmD20-c5  (40) mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2  30 tumorous blood cells mbn2  (57) ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    3 embryonic 1182-4H  (174) embryonic GM2    61 embryonic Kc167    17 embryonic S1    85 embryonic S3    9 leg disc CME L1    5 wing disc CME-W2    40 wing disc ML-DmD8    31 wing disc ML-DmD9    39 wing disc ML-DmD16-c3    49 wing disc ML-DmD21    1 wing disc ML-DmD32    37 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    98 antennal disc ML-DmD20-c5    40 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    57 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    3 embryonic 1182-4H    174 embryonic GM2    61 embryonic Kc167    17 embryonic S1    85 embryonic S3    9 leg disc CME L1    5 wing disc CME-W2    40 wing disc ML-DmD8    31 wing disc ML-DmD9    39 wing disc ML-DmD16-c3    49 wing disc ML-DmD21    1 wing disc ML-DmD32    37 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    98 antennal disc ML-DmD20-c5    40 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    57 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    16 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult    51 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    30 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    32 Ethanol 2.5% 3 hrs, larvae L3    48 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    38 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    47 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    16 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult  (51) Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult  (30) Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult  (32) Ethanol 2.5% 3 hrs, larvae L3  (48) Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (38) Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult  (44) Paraquat 10 mM 48 hrs, 4-day adult  (47) Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    16 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult    51 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    30 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    32 Ethanol 2.5% 3 hrs, larvae L3    48 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    38 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    47 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    16 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult    51 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    30 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    32 Ethanol 2.5% 3 hrs, larvae L3    48 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    38 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    47 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    16 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult    51 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    30 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    32 Ethanol 2.5% 3 hrs, larvae L3    48 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    38 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    47 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    16 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult  (51) Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult  30 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult  32 Ethanol 2.5% 3 hrs, larvae L3  (48) Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (38) Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult  (44) Paraquat 10 mM 48 hrs, 4-day adult  (47) Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    16 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult    51 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    30 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    32 Ethanol 2.5% 3 hrs, larvae L3    48 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    38 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    47 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    16 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    21 Cadmium 100 mM 48 hrs, 4-day adult    51 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    30 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    32 Ethanol 2.5% 3 hrs, larvae L3    48 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    38 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    47 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_24 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts	BDGP expression data • FI03875 SD10157 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003475 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10076-RD;CG10076-RA;CG10076-RD FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for spir Stages(s) 1-3 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | germline clone | non-rescuable maternal effect | recessive spir2L-62-29 spir2L-75-28 spir2L-133-31 spir2L-146-30 spir2L-210-2 spir2L-216-18 spir2L-244-35 lethal | maternal effect | recessive spir1 spir5 spir6 lethal | recessive spirPJ2 spirPJ17 spirPJ19 viable, with Scer\GAL4pnr-MD237 spirGD6626 viable (with Df(2L)pr9201) spir08327b viable (with Df(2L)pr11163) spir08327b viable (with Df(2L)TW50) spir08327b viable (with Df(2L)TW65) spir08327b Sterility Allele female sterile spir1 spir2 spir3 spir08327b spirPJ2 spirPJ17 spirPJ19 spirPJ56 grandchildless spirZ2-4614 Other Phenotypes Allele oxidative stress response defective spirMB05102 Phenotype manifest in Allele blastoderm embryo | maternal effect spir1 chorion | maternal effect spir1 spir5 spir6 dorsal appendage | maternal effect spir1 spir5 spir6 egg | maternal effect spir1 spir5 spir6 egg chamber spir1 egg chamber | maternal effect spir1 spir5 spir6 embryo | germline clone | maternal effect | posterior spir2L-133-31 spir2L-146-30 spir2L-210-2 spir2L-216-18 spir2L-244-35 spir2L-62-29 spir2L-75-28 embryo | maternal effect spir1 spir5 spir6 embryonic abdomen | maternal effect spir1 spir5 spir6 filamentous actin | oogenesis stage S9 spir1 follicle cell | maternal effect spir1 spir5 spir6 karyosome spir1 oocyte | oogenesis stage S9 spir1 oocyte nucleus spir1 pole cell | maternal effect spir1 spir5 spir6 stage S8 oocyte & microtubule spir242 spirEC
Classical Alleles ( 48 )
For All Classical Alleles Show
Allele of spir Class Mutagen Stocks Known lesion spir1 ethyl methanesulfonate 2 Yes spirKG00320 P-element activity 2 -- spirNP2788 2 -- spir2F 1 Yes spir5-SZ-3364 1 -- spird02631 P-element activity 1 -- spirf00060 piggyBac transposase 1 -- spirf07723 piggyBac transposase 1 -- spirI83 PM hybrid dysgenesis 1 Yes spirMB05102 1 -- spirMI03254 1 -- spirMI05646 1 -- spirNP5292 1 -- spir08327b P-element activity 0 Yes spir10 ethyl methanesulfonate 0 -- spir11 ethyl methanesulfonate 0 -- spir12 ethyl methanesulfonate 0 -- spir242 0 -- spir2 ethyl methanesulfonate 0 -- spir2L-133-31 ethyl methanesulfonate 0 -- spir2L-146-30 ethyl methanesulfonate 0 -- spir2L-210-2 ethyl methanesulfonate 0 -- spir2L-216-18 ethyl methanesulfonate 0 -- spir2L-244-35 ethyl methanesulfonate 0 -- spir2L-62-29 ethyl methanesulfonate 0 -- spir2L-75-28 ethyl methanesulfonate 0 -- spir334 0 -- spir3 ethyl methanesulfonate 0 -- spir4 ethyl methanesulfonate 0 Yes spir5 ethyl methanesulfonate 0 -- spir6 ethyl methanesulfonate 0 -- spir7 ethyl methanesulfonate 0 -- spir8 ethyl methanesulfonate 0 -- spir9 ethyl methanesulfonate 0 -- spirCB-0484-3 0 -- spird07276 0 -- spirDG07808 0 -- spirEC 0 -- spirex18 0 -- spirf03644 piggyBac transposase 0 -- spirMI05505 0 -- spirMM36 P-element activity 0 Yes spirPJ17 P-element activity 0 Yes spirPJ19 P-element activity 0 Yes spirPJ2 P-element activity 0 Yes spirPJ56 ethyl methanesulfonate 0 Yes spirunspecified 0 -- spirZ2-4614 ethyl methanesulfonate 0 --
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of spir Class Mutagen Stocks Known lesion spirRC.Scer\UAS.P\T.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 2 Yes spirGD6626 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spirGL01503 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spirJF03233 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spirKK109380 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spirL.Scer\UAS.T:Avic\GFP in vitro construct - coding region fusion in vitro construct - regulatory fusion 1 Yes spirRD.Scer\UAS.P\T.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes spirS.Scer\UAS.T:Avic\GFP in vitro construct - coding region fusion in vitro construct - regulatory fusion 1 Yes spirNIG.10076R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel6046 (Ryder, 2004.4.26) Df(2L)M49 (Neuman-Silberberg and Schupbach, 1993) Df(2L)PJ2 (Bai and Tolias, 2000) Df(2L)PJ17 (Bai and Tolias, 2000) Df(2L)PJ19 (Bai and Tolias, 2000) Df(2L)pr2b (Butler et al., 2001) Df(2L)pr11 (Butler et al., 2001) Df(2L)pr21 (Manseau and Schupbach, 1989) Df(2L)pr26 (Manseau and Schupbach, 1989) Df(2L)pr37 (Butler et al., 2001) Df(2L)pr40 (Butler et al., 2001) Df(2L)pr8311 (Butler et al., 2001) Df(2L)pr9201 (Butler et al., 2001) Df(2L)pr11163 (Butler et al., 2001) Df(2L)pr-A16 (Butler et al., 2001) Df(2L)TW2 (Schupbach and Wieschaus, 1991) Df(2L)TW65 (Butler et al., 2001, Schupbach and Wieschaus, 1991) Df(2L)TW161 (Schupbach and Wieschaus, 1991)
Not disrupted in	Df(2L)DS5 (Butler et al., 2001) Df(2L)DS6 (Butler et al., 2001) Df(2L)DS9 (Butler et al., 2001) Df(2L)Fs(2)Ket-RX32 (Butler et al., 2001) Df(2L)pr1 (Butler et al., 2001) Df(2L)pr7 (Butler et al., 2001) Df(2L)pr1123 (Butler et al., 2001) Df(2L)pr9202 (Butler et al., 2001) Df(2L)pr-A20 (Butler et al., 2001) Df(2L)Sd14 (Butler et al., 2001) Df(2L)Sd57 (Butler et al., 2001) Df(2L)Sd77 (Butler et al., 2001) Df(2L)TW9 (Butler et al., 2001) Df(2L)TW50 (Butler et al., 2001) Df(2L)TW150 (Butler et al., 2001) Df(2L)VA12 (Butler et al., 2001) Df(2L)VA19 (Butler et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD6626} NA P{KK109380} NA P{NIG.10076R} NA P{TRiP.GL01503} NA P{TRiP.JF03233} NA P{UAS-spir.L.GFP} NA P{UAS-spir.S.GFP} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}spirMI03254 NA Mi{MIC}spirMI05505 NA Mi{MIC}spirMI05646 NA P{}spirMM36 NA P{RS3}spirCB-0484-3 NA P{RS5}spir5-SZ-3364 NA P{SUPor-P}spirKG00320 NA P{wHy}spirDG07808 NA PBac{WH}spirf00060 NA PBac{WH}spirf03644 NA PBac{WH}spirf07723 NA roo{}spirI83 NA insertion of enhancer trap binary system Mi{ET1}spirMB05102 No P{GawB}spirNP2788 No P{GawB}spirNP5292 No insertion of mobile activating element P{XP}spird02631 NA P{XP}spird07276 NA insertion of enhancer trap P{PZ}PJ2 No P{PZ}spir08327b No
Gene Ontology: Function, Process & Cellular Component ( 17 unique terms )
Terms Based on Experimental Evidence ( 12 terms )
Molecular Function
CV term Evidence References
microtubule binding inferred from mutant phenotype (Rosales-Nieves et al., 2006) protein binding inferred from physical interaction with egg (Stabell et al., 2006) inferred from physical interaction with mon2 (Tanaka et al., 2011) inferred from physical interaction with wash (Liu et al., 2009)
Biological Process
CV term Evidence References
actin filament-based process inferred from direct assay (Rosales-Nieves et al., 2006) actin filament organization inferred from mutant phenotype (Dahlgaard et al., 2007) actin nucleation inferred from direct assay (Rosales-Nieves et al., 2006) chorion-containing eggshell formation inferred from mutant phenotype (Schupbach and Wieschaus, 1991) karyosome formation inferred from mutant phenotype (Djagaeva et al., 2005) pole plasm assembly inferred from mutant phenotype (Raff et al., 1990, Schupbach and Wieschaus, 1991) pole plasm RNA localization inferred from mutant phenotype (Raff et al., 1990) regulation of cytoskeleton organization inferred from direct assay (Liu et al., 2009)
Cellular Component
CV term Evidence References
cell cortex inferred from direct assay (Rosales-Nieves et al., 2006) cytoplasm inferred from direct assay (Rosales-Nieves et al., 2006)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
actin binding inferred from electronic annotation with InterPro:IPR003124 (FlyBase Curators et al., 2004-) non-traceable author statement (Wellington, 1999.9.10) traceable author statement (Johnstone and Lasko, 2001)
Biological Process
CV term Evidence References
actin cytoskeleton organization inferred from sequence or structural similarity (Kerkhoff, 1999.5.20) traceable author statement (Ciccarelli et al., 2003) oogenesis traceable author statement (Wylie, 1999) pole plasm assembly non-traceable author statement (Williamson and Lehmann, 1996) traceable author statement (Wilsch-Brauninger et al., 1997, Mahowald, 2001) pole plasm oskar mRNA localization traceable author statement (Johnstone and Lasko, 2001) vesicle-mediated transport traceable author statement (Ciccarelli et al., 2003)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene_with_edited_transcript (Stapleton et al., 2006, Graveley et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
spir - Act5C x ray scattering (Sitar et al., 2011) spir - mon2 anti tag coimmunoprecipitation, anti tag western blot, two hybrid (Tanaka et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions spir allele Gene References spir1 Cf2 (Hsu et al., 1996) nos (Wang and Lehmann, 1991) unspecified Egfr (Manseau and Schupbach, 1989) grk (Manseau and Schupbach, 1989)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 69994 DroID - A comprehensive database of gene and protein interactions. • FBgn0003475 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45931
Orthologs
OrthoDB Orthologs (36) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6G7CHQ Dipteran inclusive ortholog search EOG6B5PD7 Insect inclusive ortholog search EOG6M0CXB Arthropod inclusive ortholog search EOG6M9072 Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6G7CHQ)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\spir     Drosophila simulans   Dsim\GD24252 Y   Drosophila sechellia   Dsec\GM23344 Y   Drosophila erecta   Dere\GG21227 Y   Drosophila yakuba   Dyak\GE13304 Y   Drosophila ananassae   Dana\GF14534 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10053 Y   Drosophila persimilis   Dper\GL26604 Y   Drosophila willistoni   Dwil\GK18263 Y   Drosophila virilis   Dvir\GJ23310 Y   Drosophila mojavensis   Dmoj\GI22759 Y   Drosophila grimshawi   Dgri\GH13128 Y
Orthologs in non-Drosophila Dipterans (EOG6B5PD7)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL006289   Anopheles gambiae Malaria mosquito  Agam\AGAP008100   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ011319   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ008348
Orthologs in non-Dipteran Insects (EOG6M0CXB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB45684   Apis mellifera Western honey bee  Amel\GB45685   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG020351   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG026831   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10040   Atta cephalotes Leafcutter ant  Acep\ACEP22174   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17343   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL20523   Linepithema humile Argentine ant  Lhum\LH19097   Linepithema humile Argentine ant  Lhum\LH19098   Pogonomyrmex barbatus Red harvester ant  Pbar\PB13122   Pogonomyrmex barbatus Red harvester ant  Pbar\PB13128   Solenopsis invicta Red fire ant  Sinv\SINV17038   Solenopsis invicta Red fire ant  Sinv\SINV17037   Acyrthosiphon pisum Pea aphid  Apim\ACYPI56640   Bombyx mori Silkmoth  Bmor\BGIBMGA003093   Pediculus humanus Human body louse  Phum\PHUM135880   Tribolium castaneum Red flour beetle  Tcas\spir
Orthologs in non-Insect Arthropods (EOG6M9072)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\COII   Ixodes scapularis Deer tick  Isca\ISCW003557
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD24252 Drosophila sechellia Dsec\GM23344 Drosophila erecta Dere\GG21227 Drosophila yakuba Dyak\GE13304 Drosophila ananassae Dana\GF14534 Drosophila pseudoobscura pseudoobscura Dpse\GA10053 Drosophila persimilis Dper\GL26604 Drosophila willistoni Dwil\GK18263 Drosophila virilis Dvir\GJ23310 Drosophila mojavensis Dmoj\GI22759 Drosophila grimshawi Dgri\GH13128
Stocks & Reagents
Stocks Listed in FlyBase ( 25 )
Bloomington	24765 w*; P{UASp-spir.RC.GFP}21 24766 w*; P{UASp-spir.RC.GFP}32 24767 w*; P{UASp-spir.GFP.RD}30 24237 w1118; Mi{ET1}spirMB05102 30516 y1 v1; P{TRiP.JF03233}attP2 43161 y1 v1; P{TRiP.GL01503}attP2
Harvard	- P{XP}spird02631 - PBac{WH}spirf00060 - PBac{WH}spirf07723
Kyoto	126020 w1118; P{RS5}spir5-SZ-3364
VDRC	v107335 P{KK109380}VIE-260B v44092 w1118; P{GD6626}v44092
	More stocks available...
Genomic Clones ( 2 )
	BACR03G24 BACR25N15 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 119 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT09970 GH13327 LD37577 SD10157
Other clones	FI03875 MIP18615 MIP21567 MIP21867
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT02483 AT04592 AT04805 AT04983 AT08394 AT16163 AT16350 AT16890 AT17454 AT17589 AT20988 AT21315 AT21412 AT24501 AT27880 AT30330 GH01380 GH07234 GH10380 GH10722 GH16981 GM02734 GM10473 GM19858 LD09627 LD46011 LP16355 LP20763 RE03740 RE09756 RE10811 RE20069 RE26938 RE29721 RE32684 RE56142 RE56907 RE57650 RH13949 RH14424 RH33305 RH35333 RH38593 RH38705 RH57620 RH58568 RH62624 RH66806 RH67403 RH69801 SD21040 SD25214
Other clones	260 364 31706 37554 38437 43817 44585 51083 55544 57272 64322 74769 79462 162380 174537 184411 190099 192356 214251.8 283268 AT01308 AT01309 bs13b10 bs14e03 bs53a06 bs59g02 CK01.2B.D9 CK01.2D.H12 CK01.19A.H2 CK01.34B.C10 CK01.55B.B2 CK01.75D.G9 CK01.106A.A12 CK01.115A.D1 CK01.169D.H2 EK007011 EK044937 EK059227 EK088634 EK107016 EK107161 EK141237 EK151558 EK155754 EK158610 EK160134 EK160334 EK161914 EK171650 EK177415 EK181235 EK182346 EK189846 EK209349 EK266153 EK289442 EK292310 EK295337 EK307930
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003475 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45931
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: spir CG10076 (Whitfield, 2000.5.15) Source for merge of: spir CG18621 (Whitfield, 2000.12.15) Source for merge of: spir Spir (FlyBase, 1996-)
Additional comments	Source for merge of spir Spir was sequence comparison (date:000414). (FlyBase, 1996-)
Other Comments
	The spir-RC isoform crosslinks microtubules and microfilaments at the oocyte cortex; the spir-RD isoform can bind the spir-RC isoform and prevent its microfilament crosslinking activity. (Rosales-Nieves et al., 2006) dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation. (Rogers et al., 2003) Area matching Drosophila EST AA390587. (Davies, 2001.3.15) The spir protein links rho family signalling to the actin cytoskeleton. (Wellington et al., 1999) The chic mutant phenotype resembles that caused by disrupting the actin cytoskeleton with cytochalasin D, and that of capu and spir. (Manseau et al., 1996) capu and spir are not required for the synthesis or nuclear localisation of fs(1)K10 protein and they mediate dorsoventral patterning independently of fs(1)K10 mRNA localisation. (Serano and Cohen, 1995) Only vas and tud are essential for osk-induced pole cell and abdomen formation, spir is not. (Ephrussi and Lehmann, 1992) vas, vls and tud (but not stau, capu, or spir) are necessary for the 6xosk mutant phenotypes. (Smith et al., 1992) spir mutant embryos exhibit an absence of posterior pole plasm, polar granules and pole cells. BicD,spir embryos exhibit a duplication of the posterior telson at the anterior: the posterior signal can be active at the anterior independent of spir function. spir is required specifically for the localization, not synthesis, of the posterior signal. (Lehmann and Nusslein-Volhard, 1991) Mutations in maternal dorsal class gene spir do not interact with RpII140wimp. (Parkhurst and Ish-Horowicz, 1991) Mutations at the spir locus cause defects in midoogenesis. (Schupbach and Wieschaus, 1991) spir gene function affects dorsoventral and anteroposterior patterning. (Lasko and Ashburner, 1990) spir plays a role in polar granule formation. (Raff et al., 1990) Mutations in grk and Egfr are epistatic to mutations in capu and spir. (Manseau and Schupbach, 1989) capu and spir participate in pattern formation in both the dorsoventral and anteroposterior axes of the embryo. Mosaic analysis reveals that the products of both are required in the oocyte-nurse cell complex. (Manseau and Schupbach, 1989)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080115 NM_165323 NM_165324 NM_165325 NM_001169549 NM_001259172 NM_001259173 NM_001259174 RefSeq (Proteins) • NP_524854 NP_724254 NP_724255 NP_724256 NP_001163020 NP_001246101 NP_001246102 NP_001246103 DDBJ / EMBL / GenBank • AA390587 AAF23615 AAF23616 AAF53884 AAL68390 AAL90241 AAN11070 AAN11071 AAN11072 AC002503 AC004735 ACZ94306 AF184975 AF184976 AFH03775 AFH03776 AFH03777 AI113886 AI135528 AI513246 AI518150 AI543415 AI945463 AI945583 AJ238876 AW944620 AX093986 AY075586 AY089503 BG638840 BH610047 CAB62901 CZ472921 CZ472922 CZ482545 CZ486220 CZ490456 CZ490457 Entrez Gene - A searchable database of RefSeq genes. • 45931 UniProtKB/Swiss-Prot • Q9U1K1 UniProtKB/TrEMBL • E1JHM3
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • WH2 domain (IPR003124) KIND (IPR011019) Zinc finger, FYVE/PHD-type (IPR011011) Zinc finger, RING/FYVE/PHD-type (IPR013083) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • FI03875 SD10157
Linkouts
	BioGRID - A database of protein and genetic interactions • 69994 DroID - A comprehensive database of gene and protein interactions. • FBgn0003475 DRSC - Results from RNAi screens. • FBgn0003475 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003475 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10076-RD;CG10076-RA;CG10076-RD FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003475 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45931 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/spire1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45931 modMine - Data generated by the modENCODE project. • FBgn0003475
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	38C.34 (McGill Drosophila Genome Project, 2001.9.25, Butler, 2001, Butler et al., 2001) 38C.37 (McGill Drosophila Genome Project, 2001.9.25, Butler, 2001, Butler et al., 2001) CG10076 (Whitfield, 2000.5.15, Whitfield, 2000.5.15, Rogers et al., 2003, Perkins et al., 2009, Keleman et al., 2009.8.5, Aerts et al., 2010, Ni et al., 2010.12.1) CG18621   l(2)08327 (Butler, 2001) NP2788 (Strand and Micchelli, 2011) p150Spir (Xia and Karin, 2004) p150-Spir (Ciccarelli et al., 2003, Jacinto et al., 2002, Van Aelst and Symons, 2002, Paunola et al., 2002, Otto, 2000, Otto et al., 2000, Kerkhoff, 1999.5.20) Spir (Dahlgaard et al., 2007, Tanaka et al., 2011) spir (Stapleton et al., 2006, Neuman-Silberberg, 2007, Stabell et al., 2006, Lecuyer et al., 2007, Guan et al., 2011, Hazelett et al., 2012, Liu et al., 2009, Wang et al., 1994, Girardot et al., 2006, Xi et al., 2008, Yassin et al., 2010, Aerts et al., 2010, Wasbrough et al., 2010, Japanese National Institute of Genetics, 2012.5.21, Jordan et al., 2012, Chen et al., 2012) Spire (Kerkhoff, 2006, Sitar et al., 2011) spire (Wang and Riechmann, 2008)
Name Synonym	spire (Kerkhoff, 2006, Webster et al., 1997, McGrail et al., 1995, Tsuda et al., 2006, Wang and Riechmann, 2007, Neuman-Silberberg, 2007, Stabell et al., 2006, Wang and Riechmann, 2008, Chang et al., 2011, Guan et al., 2011, Borghese et al., 2006, Dahlgaard et al., 2007, Quinlan et al., 2007, Tanaka and Nakamura, 2008, Hurlbut et al., 2009, Levine et al., 2007, Johansson et al., 2005) Spire (Rogers et al., 2003, Baum and Kunda, 2005, Stroupe et al., 2009, Liu et al., 2009, Ducka et al., 2010, Chen et al., 2012, Felix et al., 2012)
Secondary FlyBase IDs
	FBgn0029122 FBgn0032854 FBgn0040997
References ( 203 )
Generate a list of	All references relating to this gene ( 203 )
List References by type	Research paper  ( 97 ) Supplementary material  ( 12 ) Review  ( 54 ) Personal communication to FlyBase  ( 20 ) Abstract  ( 10 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 3 ) Stock list  ( 1 ) Patent  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 10 )
	Chen et al., 2012, J. Biol. Chem. 287(13): 10684--10692 Multiple Forms of Spire-Actin Complexes and their Functional Consequences. [FBrf0217866] Felix et al., 2012, Genetics 191(3): 989--1002 Age-Specific Variation in Immune Response in Drosophila melanogaster Has a Genetic Basis. [FBrf0218944] Hazelett et al., 2012, G3 (Bethesda) 2(7): 789--802 Comparison of Parallel High-Throughput RNA Sequencing Between Knockout of TDP-43 and Its Overexpression Reveals Primarily Nonreciprocal and Nonoverlapping Gene Expression Changes in the Central Nervous System of Drosophila. [FBrf0219102] Jordan et al., 2012, PLoS ONE 7(6): e38722 Genome-Wide Association for Sensitivity to Chronic Oxidative Stress in Drosophila melanogaster. [FBrf0218606] Chang et al., 2011, Genetics 188(4): 883--896 Anterior-Posterior Axis Specification in Drosophila Oocytes: Identification of Novel bicoid and oskar mRNA Localization Factors. [FBrf0214626] Gates et al., 2011, Neural Dev. 6: 37 A genome-wide analysis reveals that the Drosophila transcription factor Lola promotes axon growth in part by suppressing expression of the actin nucleation factor Spire. [FBrf0217266] Guan et al., 2011, Learn. Mem. 18(4): 191--206 Altered gene regulation and synaptic morphology in Drosophila learning and memory mutants. [FBrf0213277] Sitar et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(49): 19575--19580 Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly. [FBrf0218145] Strand and Micchelli, 2011, Proc. Natl. Acad. Sci. U.S.A. 108(43): 17696--17701 Quiescent gastric stem cells maintain the adult Drosophila stomach. [FBrf0216495] Tanaka et al., 2011, Development 138(12): 2523--2532 Drosophila Mon2 couples Oskar-induced endocytosis with actin remodeling for cortical anchorage of the germ plasm. [FBrf0213769] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...