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Gene Dmel\spn-A

General Information
Symbol	Dmel\spn-A	Species	D. melanogaster
Name	spindle A	Annotation symbol	CG7948
Feature type	protein_coding_gene	FlyBase ID	FBgn0003479
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	Rad51, spnA, DmRad51, DMR
Genomic Location
Chromosome (arm)	3R	Recombination map	3-96
Cytogenetic map	99D3-99D3	Sequence location	3R:25,880,418..25,881,759 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene spindle A is referred to in FlyBase by the symbol Dmel\spn-A (CG7948, FBgn0003479). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: germarium-derived oocyte fate determination; female meiosis; double-strand break repair via synthesis-dependent strand annealing; polarity specification of dorsal/ventral axis; intracellular mRNA localization; cell cycle checkpoint; oogenesis; polarity specification of anterior/posterior axis; karyosome formation. 31 alleles are reported. The phenotypes of these alleles are annotated with: germline cyst; chorion; egg; oocyte; presumptive oocyte; karyosome; egg chamber; dorsal appendage; nurse cell; synaptonemal complex. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: AAA+ ATPase domain; DNA recombination and repair protein Rad51, C-terminal; DNA recombination and repair protein, RecA-like; DNA recombination/repair protein Rad51; DNA recombination/repair protein RecA, monomer-monomer interface; DNA recombination/repair protein RecA/RadB, ATP-binding domain; DNA repair Rad51/transcription factor NusA, alpha-helical; Helix-hairpin-helix DNA-binding motif, class 1. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1640738_a_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of spn-A. Gene sequence location is 3R:25880418..25881759.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	spn-A: spindle A Maternal-effect lethal. Egg shape affected; in extreme cases dorsal appendages are lacking and eggs have little or no dorsal-ventral polarity; some eggs have one fused dorsal appendage. Low fecundity; eggs often slightly collapsed.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 99D3-99D3   Limits computationally determined from genome sequence between P{PZ}ncd05884 and P{lacW}l(3)s2500s2500
Experimentally Determined Cytological Location
Cytogenetic map Notes References 99D-99D     (Akaboshi, 1995) 93D-93D   (determined by in situ hybridisation)   (McKee et al., 1996) 99D-99D   (determined by in situ hybridisation)   (Akaboshi et al., 1994) 99D3-99D6     (McKee, 1995.6.8)
Experimentally Determined Recombination Data
Location	3-96 (Tearle and Nusslein-Volhard, 1987, Gonzalez-Reyes et al., 1997) 3- (Akaboshi, 1995)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\spn-A for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43 Gene model reviewed during 5.47 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) spn-A-RA FBtr0085589  NM_079844   1279   336 spn-A-RB FBtr0085590  NM_170463   1342   279
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein spn-A-PA   FBpp0084955   36.6   336   6.52     NP_524583   AAF57005 spn-A-PB   FBpp0084956   30.5   279   7.42     NP_733342   AAN14213
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • AAA+ ATPase domain (IPR003593) DNA repair Rad51/transcription factor NusA, alpha-helical (IPR010995) DNA recombination/repair protein Rad51 (IPR011941) DNA recombination and repair protein Rad51, C-terminal (IPR013632) Helix-hairpin-helix DNA-binding motif, class 1 (IPR003583) DNA recombination and repair protein, RecA-like (IPR016467) DNA recombination/repair protein RecA, monomer-monomer interface (IPR020587) DNA recombination/repair protein RecA/RadB, ATP-binding domain (IPR020588) P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA979378   AE014297 AAF57005   AE014297 AAN14213   AI259851   AI455994   AI512877   BG638776   BG640472   BI574541   BI584842   BI636986   BP545902   BQ102918   BT001791 AAN71546   BT089037   CK131377   CK658856   CK659360   CO299430   D17726 BAA04580   D37788 BAA07039   EC199700   L41342 AAA64873
UniProtKB/Swiss-Prot	Q27297
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) central brain primordium   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    85.35 Larval Midgut    46.8 Larval Hindgut    56.3 Larval Malpighian Tubules    77.5 Larval Fat Body    55.9 Larval Salivary Gland    45.3 Larval Trachea    73.725 Larval Carcass    44.275 Adult Head    36.7 Adult Eye    41.125 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    52.4 Adult Crop    61.7 Adult Midgut    44.2 Adult Hindgut    46.4 Adult Malpighian Tubules    35.3 Adult Fat Body    27.5 Adult Salivary Gland    34.1 Adult Heart    32.15 Adult VirginFemale Spermatheca    32.3 Adult InseminatedFemale Spermatheca    33.4 Adult Ovary    179.9 Adult Testis    59.9 Adult Male Accessory Gland    54.4 Adult Carcass    43.8 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    85.35 Larval Midgut    46.8 Larval Hindgut    56.3 Larval Malpighian Tubules    77.5 Larval Fat Body    55.9 Larval Salivary Gland    45.3 Larval Trachea    73.725 Larval Carcass    44.275 Adult Head    36.7 Adult Eye    41.125 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    52.4 Adult Crop    61.7 Adult Midgut    44.2 Adult Hindgut    46.4 Adult Malpighian Tubules    35.3 Adult Fat Body    27.5 Adult Salivary Gland    34.1 Adult Heart    32.15 Adult VirginFemale Spermatheca    32.3 Adult InseminatedFemale Spermatheca    33.4 Adult Ovary    179.9 Adult Testis    59.9 Adult Male Accessory Gland    54.4 Adult Carcass    43.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    85.35 Larval Midgut    46.8 Larval Hindgut    56.3 Larval Malpighian Tubules    77.5 Larval Fat Body    55.9 Larval Salivary Gland    45.3 Larval Trachea    73.725 Larval Carcass    44.275 Adult Head    36.7 Adult Eye    41.125 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    52.4 Adult Crop    61.7 Adult Midgut    44.2 Adult Hindgut    46.4 Adult Malpighian Tubules    35.3 Adult Fat Body    27.5 Adult Salivary Gland    34.1 Adult Heart    32.15 Adult VirginFemale Spermatheca    32.3 Adult InseminatedFemale Spermatheca    33.4 Adult Ovary    179.9 Adult Testis    59.9 Adult Male Accessory Gland    54.4 Adult Carcass    43.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    85.35 Larval Midgut    46.8 Larval Hindgut    56.3 Larval Malpighian Tubules    77.5 Larval Fat Body    55.9 Larval Salivary Gland    45.3 Larval Trachea    73.725 Larval Carcass    44.275 Adult Head    36.7 Adult Eye    41.125 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    52.4 Adult Crop    61.7 Adult Midgut    44.2 Adult Hindgut    46.4 Adult Malpighian Tubules    35.3 Adult Fat Body    27.5 Adult Salivary Gland    34.1 Adult Heart    32.15 Adult VirginFemale Spermatheca    32.3 Adult InseminatedFemale Spermatheca    33.4 Adult Ovary    179.9 Adult Testis    59.9 Adult Male Accessory Gland    54.4 Adult Carcass    43.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    85.35 Larval Midgut    46.8 Larval Hindgut    56.3 Larval Malpighian Tubules    77.5 Larval Fat Body    55.9 Larval Salivary Gland    45.3 Larval Trachea    73.725 Larval Carcass    44.275 Adult Head    36.7 Adult Eye    41.125 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    52.4 Adult Crop    61.7 Adult Midgut    44.2 Adult Hindgut    46.4 Adult Malpighian Tubules    35.3 Adult Fat Body    27.5 Adult Salivary Gland    34.1 Adult Heart    32.15 Adult VirginFemale Spermatheca    32.3 Adult InseminatedFemale Spermatheca    33.4 Adult Ovary    179.9 Adult Testis    59.9 Adult Male Accessory Gland    54.4 Adult Carcass    43.8 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    85.35 Larval Midgut    46.8 Larval Hindgut    56.3 Larval Malpighian Tubules    77.5 Larval Fat Body    55.9 Larval Salivary Gland    45.3 Larval Trachea    73.725 Larval Carcass    44.275 Adult Head    36.7 Adult Eye    41.125 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    52.4 Adult Crop    61.7 Adult Midgut    44.2 Adult Hindgut    46.4 Adult Malpighian Tubules    35.3 Adult Fat Body    27.5 Adult Salivary Gland    34.1 Adult Heart    32.15 Adult VirginFemale Spermatheca    32.3 Adult InseminatedFemale Spermatheca    33.4 Adult Ovary    179.9 Adult Testis    59.9 Adult Male Accessory Gland    54.4 Adult Carcass    43.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    85.35 Larval Midgut    46.8 Larval Hindgut    56.3 Larval Malpighian Tubules    77.5 Larval Fat Body    55.9 Larval Salivary Gland    45.3 Larval Trachea    73.725 Larval Carcass    44.275 Adult Head    36.7 Adult Eye    41.125 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    52.4 Adult Crop    61.7 Adult Midgut    44.2 Adult Hindgut    46.4 Adult Malpighian Tubules    35.3 Adult Fat Body    27.5 Adult Salivary Gland    34.1 Adult Heart    32.15 Adult VirginFemale Spermatheca    32.3 Adult InseminatedFemale Spermatheca    33.4 Adult Ovary    179.9 Adult Testis    59.9 Adult Male Accessory Gland    54.4 Adult Carcass    43.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    85.35 Larval Midgut    46.8 Larval Hindgut    56.3 Larval Malpighian Tubules    77.5 Larval Fat Body    55.9 Larval Salivary Gland    45.3 Larval Trachea    73.725 Larval Carcass    44.275 Adult Head    36.7 Adult Eye    41.125 Adult Brain    40.7 Adult Thoracic-Abdominal Ganglion    52.4 Adult Crop    61.7 Adult Midgut    44.2 Adult Hindgut    46.4 Adult Malpighian Tubules    35.3 Adult Fat Body    27.5 Adult Salivary Gland    34.1 Adult Heart    32.15 Adult VirginFemale Spermatheca    32.3 Adult InseminatedFemale Spermatheca    33.4 Adult Ovary    179.9 Adult Testis    59.9 Adult Male Accessory Gland    54.4 Adult Carcass    43.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    36.7   NA  Eye    41.125   NA  Brain    40.7   85.35  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    52.4   NA  Crop    61.7   46.8  Midgut    44.2   56.3  Hindgut    46.4   77.5  Malpighian Tubules    35.3   55.9  Fat Body    no informative data   no informative data  Salivary Gland    no informative data   NA  Heart    32.15   73.725  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    33.4   NA  Ovary    179.9   NA  Testis    59.9   NA  Male Accessory Gland    54.4   44.275  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    11 ovary, mated 4-day female    10 testis, mated 4-day male    8 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    11 ovary, mated 4-day female    10 testis, mated 4-day male    8 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    11 ovary, mated 4-day female    10 testis, mated 4-day male    8 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    11 ovary, mated 4-day female    10 testis, mated 4-day male    8 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    11 ovary, mated 4-day female    10 testis, mated 4-day male    8 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    11 ovary, mated 4-day female    10 testis, mated 4-day male    8 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    11 ovary, mated 4-day female    10 testis, mated 4-day male    8 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    11 ovary, mated 4-day female    10 testis, mated 4-day male    8 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    43 embryo 02-04hr    20 embryo 04-06hr    26 embryo 06-08hr    22 embryo 08-10hr    18 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    11 adult female 05day    17 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (43) embryo 02-04hr    20 embryo 04-06hr  26 embryo 06-08hr    22 embryo 08-10hr    18 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    11 adult female 05day    17 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    43 embryo 02-04hr    20 embryo 04-06hr    26 embryo 06-08hr    22 embryo 08-10hr    18 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    11 adult female 05day    17 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    43 embryo 02-04hr    20 embryo 04-06hr    26 embryo 06-08hr    22 embryo 08-10hr    18 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    11 adult female 05day    17 adult female 30day    20 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    43 embryo 02-04hr    20 embryo 04-06hr    26 embryo 06-08hr    22 embryo 08-10hr    18 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    11 adult female 05day    17 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (43) embryo 02-04hr    20 embryo 04-06hr  26 embryo 06-08hr    22 embryo 08-10hr    18 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    11 adult female 05day    17 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    43 embryo 02-04hr    20 embryo 04-06hr    26 embryo 06-08hr    22 embryo 08-10hr    18 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    11 adult female 05day    17 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    43 embryo 02-04hr    20 embryo 04-06hr    26 embryo 06-08hr    22 embryo 08-10hr    18 embryo 10-12hr    14 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    8 adult female 01day    11 adult female 05day    17 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    15 embryonic 1182-4H    10 embryonic GM2    12 embryonic Kc167    15 embryonic S1    20 embryonic S3    14 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    10 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    18 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    17 ovary fGS/OSS    9 ovary OSC    8 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    15 embryonic 1182-4H    10 embryonic GM2    12 embryonic Kc167    15 embryonic S1    20 embryonic S3    14 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    10 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    18 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    17 ovary fGS/OSS    9 ovary OSC    8 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    15 embryonic 1182-4H    10 embryonic GM2    12 embryonic Kc167    15 embryonic S1    20 embryonic S3    14 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    10 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    18 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    17 ovary fGS/OSS    9 ovary OSC    8 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    15 embryonic 1182-4H    10 embryonic GM2    12 embryonic Kc167    15 embryonic S1    20 embryonic S3    14 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    10 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    18 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    17 ovary fGS/OSS    9 ovary OSC    8 ovary OSS    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    15 embryonic 1182-4H    10 embryonic GM2    12 embryonic Kc167    15 embryonic S1    20 embryonic S3    14 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    10 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    18 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    17 ovary fGS/OSS    9 ovary OSC    8 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    15 embryonic 1182-4H    10 embryonic GM2    12 embryonic Kc167    15 embryonic S1    20 embryonic S3    14 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    10 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    18 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    17 ovary fGS/OSS    9 ovary OSC    8 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    15 embryonic 1182-4H    10 embryonic GM2    12 embryonic Kc167    15 embryonic S1    20 embryonic S3    14 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    10 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    18 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    17 ovary fGS/OSS    9 ovary OSC    8 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    15 embryonic 1182-4H    10 embryonic GM2    12 embryonic Kc167    15 embryonic S1    20 embryonic S3    14 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    10 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    18 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    17 ovary fGS/OSS    9 ovary OSC    8 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_05
External Data & Images
Linkouts	BDGP expression data • RE29170 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003479 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7948-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | maternal effect spn-A1 lethal | pupal stage, with Scer\GAL4pnr-MD237 spn-AGD5050 viable (with Df(3R)X3F) spn-A1 spn-A001A spn-A2 spn-A3 spn-A009A spn-A009D spn-A010A spn-A032B spn-A048B spn-A050A spn-A055A spn-A061A spn-A068A spn-A084A spn-A087A spn-A091B spn-A091C spn-A093A spn-A095A spn-A104-7 spn-A116C spn-A119A spn-A128-53 spn-A148-15 spn-A155-52 Sterility Allele semi-fertile spn-A1 Other Phenotypes Allele DNA repair defective spn-A1 meiotic cell cycle defective | female (with Df(3R)X3F) spn-A093A radiation sensitive spn-A3 spn-A093A radiation sensitive (with Df(3R)X3F) spn-A093A radiation sensitive (with spn-A3) spn-A093A radiation sensitive (with spn-A048B) spn-A093A radiation sensitive (with spn-A093A) spn-A3 spn-A048B radiation sensitive | dominant spn-A1 Phenotype manifest in Allele chorion (with spn-A1) spn-A093A chorion (with spn-A093A) spn-A1 chorion | dorsal (with Df(3R)X3F) spn-A093A chorion | dorsal | germline clone spn-A155-52 dorsal appendage spn-A1 spn-Aunspecified dorsal appendage (with Df(3R)X3F) spn-A093A dorsal appendage | germline clone spn-A155-52 dorsal appendage | maternal effect (with spn-A1) spn-A093A dorsal appendage | maternal effect (with spn-A093A) spn-A1 egg spn-A1 spn-Aunspecified egg | dorsal spn-A2 spn-A3 egg chamber spn-A3 germline cyst spn-A3 karyosome spn-A2 spn-A3 spn-Aunspecified karyosome (with Df(3R)X3F) spn-A093A karyosome | germline clone spn-A093A nurse cell spn-A2 spn-A3 oocyte spn-A2 spn-A3 presumptive oocyte spn-A1 synaptonemal complex spn-A3 synaptonemal complex (with Df(3R)X3F) spn-A093A
Classical Alleles ( 27 )
For All Classical Alleles Show
Allele of spn-A Class Mutagen Stocks Known lesion spn-A1 ethyl methanesulfonate 1 Yes spn-A001A ethyl methanesulfonate 0 Yes spn-A009A ethyl methanesulfonate 0 -- spn-A009D ethyl methanesulfonate 0 Yes spn-A010A ethyl methanesulfonate 0 Yes spn-A032B ethyl methanesulfonate 0 Yes spn-A048B ethyl methanesulfonate 0 Yes spn-A050A ethyl methanesulfonate 0 Yes spn-A055A ethyl methanesulfonate 0 Yes spn-A061A ethyl methanesulfonate 0 Yes spn-A068A ethyl methanesulfonate 0 Yes spn-A084A ethyl methanesulfonate 0 Yes spn-A087A ethyl methanesulfonate 0 Yes spn-A091B ethyl methanesulfonate 0 Yes spn-A091C ethyl methanesulfonate 0 Yes spn-A093A ethyl methanesulfonate 0 Yes spn-A095A ethyl methanesulfonate 0 Yes spn-A104-7 ethyl methanesulfonate 0 Yes spn-A116C ethyl methanesulfonate 0 Yes spn-A119A ethyl methanesulfonate 0 Yes spn-A128-53 ethyl methanesulfonate 0 Yes spn-A148-15 ethyl methanesulfonate 0 Yes spn-A155-52 ethyl methanesulfonate 0 Yes spn-A2 ethyl methanesulfonate 0 Yes spn-A3 ethyl methanesulfonate 0 Yes spn-A4 ethyl methanesulfonate 0 -- spn-Aunspecified 0 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of spn-A Class Mutagen Stocks Known lesion spn-AGD5050 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes spn-AGL00669 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spn-AJF01716 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spn-ANIG.7948R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)X3F (Staeva-Vieira et al., 2003, Cook, 2006.1.31)
Not disrupted in	Df(3R)01215 (Cook, 2006.1.31) Df(3R)tll-g (Cook, 2006.1.31)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD5050} NA P{NIG.7948R} NA P{TRiP.GL00669} NA P{TRiP.JF01716} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 18 unique terms )
Terms Based on Experimental Evidence ( 10 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with UniProtKB:Q9W157 (assigned by UniProtKB) (Brough et al., 2008)
Biological Process
CV term Evidence References
cell cycle checkpoint inferred from mutant phenotype (Staeva-Vieira et al., 2003) double-strand break repair via synthesis-dependent strand annealing inferred from mutant phenotype (McVey et al., 2004, Wei and Rong, 2007) female meiosis inferred from mutant phenotype (Staeva-Vieira et al., 2003) germarium-derived oocyte fate determination inferred from genetic interaction with mus301 (Gonzalez-Reyes et al., 1997) intracellular mRNA localization inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) karyosome formation inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) oogenesis inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) polarity specification of anterior/posterior axis inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) polarity specification of dorsal/ventral axis inferred from mutant phenotype (Gonzalez-Reyes et al., 1997)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 10 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR011941, InterPro:IPR020587, InterPro:IPR020588 (FlyBase Curators et al., 2004-) damaged DNA binding inferred from electronic annotation with InterPro:IPR011941 (FlyBase Curators et al., 2004-) DNA-dependent ATPase activity inferred from electronic annotation with InterPro:IPR011941, InterPro:IPR020587, InterPro:IPR020588 (FlyBase Curators et al., 2004-) non-traceable author statement (Swiss-Prot Project Members, 1997.2.1) recombinase activity inferred from sequence or structural similarity with human RAD51 (Sekelsky et al., 2000)
Biological Process
CV term Evidence References
DNA recombination inferred from sequence or structural similarity with human RAD51 (Sekelsky et al., 2000) DNA repair inferred from sequence or structural similarity (Swiss-Prot Project Members, 1997.2.1) inferred from sequence or structural similarity with human RAD51 (Sekelsky et al., 2000) inferred from sequence or structural similarity with Saccharomyces RAD51 (FlyBase, 1992-) intracellular mRNA localization traceable author statement (Cooperstock and Lipshitz, 1997) oocyte differentiation non-traceable author statement (Deng and Lin, 2001) oogenesis traceable author statement (van Eeden and St. Johnston, 1999)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Swiss-Prot Project Members, 1997.2.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
spn-A - ATPsyn-γ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spn-A - Arpc1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spn-A - Arpc2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spn-A - Arpc3A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spn-A - Art8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spn-A - CG1416 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spn-A - CG4169 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spn-A - Sec13 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spn-A - isoQC anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spn-A - mts anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions spn-A allele Gene References spn-A3 Lig4 (McVey et al., 2004) spn-D (Gonzalez-Reyes et al., 1997, Huynh and St. Johnston, 2000) spn-E (Gonzalez-Reyes et al., 1997) spn-A093A Lig4 (McVey et al., 2004) mei-W68 (Staeva-Vieira et al., 2003) unspecified lok (Staeva-Vieira et al., 2003)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68433 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0003479 DroID - A comprehensive database of gene and protein interactions. • FBgn0003479 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43577
Orthologs
OrthoDB Orthologs (47) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6XSM8Z Dipteran inclusive ortholog search EOG63FH5M Insect inclusive ortholog search EOG63R2HC Arthropod inclusive ortholog search EOG6J9KFH Metazoa inclusive ortholog search EOG6B09H3
Orthologs in Drosophila Species (EOG6XSM8Z)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\spn-A     Drosophila simulans   Dsim\GD17366 Y   Drosophila sechellia   Dsec\GM12184 Y   Drosophila erecta   Dere\GG11967 Y   Drosophila yakuba   Dyak\GE23416 Y   Drosophila ananassae   Dana\GF16205 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA20711 Y   Drosophila persimilis   Dper\GL13479 Y   Drosophila willistoni   Dwil\GK13420 Y   Drosophila virilis   Dvir\GJ10382 Y   Drosophila mojavensis   Dmoj\GI23388 Y   Drosophila grimshawi   Dgri\GH18396 Y
Orthologs in non-Drosophila Dipterans (EOG63FH5M)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL006080   Anopheles gambiae Malaria mosquito  Agam\AGAP013412   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ003679
Orthologs in non-Dipteran Insects (EOG63R2HC)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Rad51   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG011722   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH17555   Atta cephalotes Leafcutter ant  Acep\ACEP15834   Camponotus floridanus Florida carpenter ant  Cflo\CFLO12797   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17306   Linepithema humile Argentine ant  Lhum\LH19187   Pogonomyrmex barbatus Red harvester ant  Pbar\PB18284   Solenopsis invicta Red fire ant  Sinv\SINV21791   Acyrthosiphon pisum Pea aphid  Apim\ACYPI008482   Bombyx mori Silkmoth  Bmor\BGIBMGA005107   Pediculus humanus Human body louse  Phum\PHUM458200   Tribolium castaneum Red flour beetle  Tcas\GLEAN_07816   Tribolium castaneum Red flour beetle  Tcas\GLEAN_07805
Orthologs in non-Insect Arthropods (EOG6J9KFH)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\v1r1   Ixodes scapularis Deer tick  Isca\ISCW001553
Orthologs in non-Arthropod Metazoa (EOG6B09H3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\rad-51   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_027921gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_019646gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009590gn   Danio rerio Zebrafish  Drer\rad51   Danio rerio Zebrafish  Drer\dmc1   Xenopus tropicalis Western clawed frog  Xtro\rad51   Gallus gallus Domestic chicken  Ggal\RAD51A   Gallus gallus Domestic chicken  Ggal\DMC1   Mus musculus House mouse  Mmus\Dmc1   Mus musculus House mouse  Mmus\Rad51   Rattus norvegicus Norway rat  Rnor\Dmc1   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000022544   Rattus norvegicus Norway rat  Rnor\Rad51   Homo sapiens Human  Hsap\RAD51   Homo sapiens Human  Hsap\DMC1
Human Orthologs (2)
Gene OMIM HGNC Hsap\RAD51     Hsap\DMC1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD17366 Drosophila sechellia Dsec\GM12184 Drosophila erecta Dere\GG11967 Drosophila yakuba Dyak\GE23416 Drosophila ananassae Dana\GF16205 Drosophila pseudoobscura pseudoobscura Dpse\GA20711 Drosophila persimilis Dper\GL13479 Drosophila willistoni Dwil\GK13420 Drosophila virilis Dvir\GJ10382 Drosophila mojavensis Dmoj\GI23388 Drosophila grimshawi Dgri\GH18396
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	38898 y1 sc* v1; P{TRiP.GL00669}attP2 31199 y1 v1; P{TRiP.JF01716}attP2/TM3, Sb1 3322 Dp(1;Y)BS; ru1 st1 e1 spn-A1 ca1/TM3, Sb1
VDRC	v13362 w1118 P{GD5050}v13362 v14021 w1118; P{GD5050}v14021/TM3
Genomic Clones ( 2 )
	BACR05L15 BACR07A18 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 16 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE29170 RH24133
Other clones	DPiM_RE29170
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD33913 LP03360 LP16118 LP17075 RH24470 SD11494 SD18854
Other clones	47199 EK175437 FGH001G04 LD35801 LD44962 UUGC0312
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003479 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43577
Antibody Information
	polyclonal (Brough et al., 2008, Chiolo et al., 2011)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Loss of spn-A sensitizes cells to DNA damage. (Brough et al., 2008) Analysis of mutants of the 5 spindle genes (spn-A, spn-B, mus301, spn-D and spn-E) reveals that the group of genes is required for each of the symmetry-breaking steps that generate polarity during axis formation. spn-E is required for the localisation of fs(1)K10, bcd and osk mRNAs, spn-A. spn-B, mus301 and spn-D play an indirect role in this process. (Gonzalez-Reyes et al., 1997) Isolation and preliminary molecular analysis of spn-A. (McKee et al., 1996) spn-A has been cloned and sequenced. (Akaboshi et al., 1994) Mutations in the spn-A, spn-B and spn-D genes result in a very similar phenotype to those in mus301, and in all cases the misplacement of the oocyte has the same consequences on the establishment of AP polarity in the egg chamber, as determined by the slbo expression in the follicle cells and the localization of osk and bcd mRNAs within the oocyte. (Gonzalez-Reyes and St. Johnston, 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079844 NM_170463 RefSeq (Proteins) • NP_524583 NP_733342 DDBJ / EMBL / GenBank • AAA64873 AAF57005 AAN14213 AAN71546 AI512877 BAA04580 BAA07039 BI584842 BT001791 D17726 D37788 L41342 Entrez Gene - A searchable database of RefSeq genes. • 43577 UniProtKB/Swiss-Prot • Q27297
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • AAA+ ATPase domain (IPR003593) DNA repair Rad51/transcription factor NusA, alpha-helical (IPR010995) DNA recombination/repair protein Rad51 (IPR011941) DNA recombination and repair protein Rad51, C-terminal (IPR013632) Helix-hairpin-helix DNA-binding motif, class 1 (IPR003583) DNA recombination and repair protein, RecA-like (IPR016467) DNA recombination/repair protein RecA, monomer-monomer interface (IPR020587) DNA recombination/repair protein RecA/RadB, ATP-binding domain (IPR020588) P-loop containing nucleoside triphosphate hydrolase (IPR027417) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE29170
Linkouts
	BioGRID - A database of protein and genetic interactions • 68433 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0003479 DroID - A comprehensive database of gene and protein interactions. • FBgn0003479 DRSC - Results from RNAi screens. • FBgn0003479 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003479 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7948-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003479 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43577 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /sturtevant/rad51spina1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43577 modMine - Data generated by the modENCODE project. • FBgn0003479
Synonyms & Secondary IDs ( 24 )
Reported As
Symbol Synonym	CG7948 (Jin et al., 2001, Ni et al., 2008, Ni et al., 2010.12.1) D37788 (Ptak and Petrov, 2002) DMR (Akaboshi, 1995, Kawasaki et al., 1996) DMR1 (Akaboshi et al., 1994) DMR/DroRAD51 (Kawasaki et al., 1998) DmRad51 (McVey et al., 2004, Pastink and Lohman, 1999) dmRAD51 (Staeva-Vieira et al., 2003, Brough et al., 2008) Dm Rad51 (Abdu et al., 2003) DmRAD51 (Staeva-Vieira et al., 2003, Vieira and Lehmann, 2002) Dmrad51 (Morris and Lehmann, 1999) dmrad51 (Brough et al., 2008) RA51 (Brocchieri and Karlin, 1998) RA51_DROME (Storm and Sonnhammer, 2003) Rad51 (Ghabrial et al., 1998, Villeneuve and Hillers, 2001, Sekelsky et al., 2000, Gene Ontology Consortium, 2000, Brendel et al., 1997, Alexiadis et al., 2004, Alexiadis and Kadonaga, 2002, Bozas and Carroll, 2006, Pawlowski and Cande, 2005, Holmes et al., 2006, Yoo and McKee, 2004, Zhai et al., 2012, Wei and Rong, 2007, Johnson-Schlitz and Engels, 2006, Bozas et al., 2009, Chan et al., 2010, Maruyama et al., 2012) RAD51 (McVey et al., 2003, McKee, 1995, Thacker, 1999) rad51 (Yu and McVey, 2010, Johnson-Schlitz et al., 2007, Klovstad et al., 2008) Rad51dm (McKee et al., 1996) spnA (McVey et al., 2004, Johnstone and Lasko, 2001, Huynh and St. Johnston, 2000, Morris et al., 2000, Ghabrial et al., 1998, Bozas and Carroll, 2006, Holmes et al., 2006, Hochwagen, 2006, Yu and McVey, 2010, Yoo, 2006, Wei and Rong, 2007, Klovstad et al., 2008, Brough et al., 2008, Navarro et al., 2009, Bozas et al., 2009, Johnson-Schlitz and Engels, 2006, Johnson-Schlitz et al., 2007, Lancaster et al., 2010, Loh et al., 2012) spn-A (Mukai et al., 2006, LaRocque et al., 2007, Zhai et al., 2012, Blanton et al., 2005, McCaffrey et al., 2006, Barbosa et al., 2007, Trowbridge et al., 2007, Toku et al., 2011, Joyce and McKim, 2009, Anderson et al., 2009, Andersen et al., 2009, Joyce et al., 2011, Chan et al., 2010, Reis et al., 2011, Andersen et al., 2011, Ferguson et al., 2012, Japanese National Institute of Genetics, 2012.5.21) Spn-A (Dworkin et al., 2009)
Name Synonym	Rad51-like   Spindle-A (Berg, 2005) spindle A (Mukai et al., 2006) spindle-A (Yoo, 2006)
Secondary FlyBase IDs
	FBgn0011700
References ( 104 )
Generate a list of	All references relating to this gene ( 104 )
List References by type	Research paper  ( 57 ) Supplementary material  ( 4 ) Review  ( 13 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 12 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 2 ) Stock list  ( 2 ) Protein sequence record  ( 1 ) Website  ( 1 ) Book  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 11 )
	Beaucher et al., 2012, Genetics 191(2): 407--417 Multiple pathways suppress telomere addition to DNA breaks in the Drosophila germline. [FBrf0218640] Ferguson et al., 2012, J. Cell Sci. 125(6): 1407--1419 Modulation of gurken translation by insulin and TOR signaling in Drosophila. [FBrf0218097] Joyce et al., 2012, Genetics 191(3): 739--746 Multiple barriers to nonhomologous DNA end joining during meiosis in Drosophila. [FBrf0218878] Loh et al., 2012, J. Cell Sci. 125(19): 4457--4462 The conserved kinase SRPK regulates karyosome formation and spindle microtubule assembly in Drosophila oocytes. [FBrf0220249] Maruyama et al., 2012, Biol. Pharmaceut. Bull. 35(11): 2017--2022 RecQ5 Interacts with Rad51 and Is Involved in Resistance of Drosophila to Cisplatin Treatment. [FBrf0219869] Zhai et al., 2012, PLoS Genet. 8(3): e1002582 Antagonistic regulation of apoptosis and differentiation by the cut transcription factor represents a tumor-suppressing mechanism in Drosophila. [FBrf0217859] Andersen et al., 2011, PLoS Genet. 7(10): e1002315 Three Structure-Selective Endonucleases Are Essential in the Absence of BLM Helicase in Drosophila. [FBrf0216460] Chiolo et al., 2011, Cell 144(5): 732--744 Double-Strand Breaks in Heterochromatin Move Outside of a Dynamic HP1a Domain to Complete Recombinational Repair. [FBrf0213146] Joyce et al., 2011, J. Cell Biol. 195(3): 359--367 Drosophila ATM and ATR have distinct activities in the regulation of meiotic DNA damage and repair. [FBrf0216489] Reis et al., 2011, PLoS ONE 6(3): e17512 Drosophila Genes That Affect Meiosis Duration Are among the Meiosis Related Genes That Are More Often Found Duplicated. [FBrf0213266] Toku et al., 2011, Comput. Biol. Chem. 35(5): 282--292 Reconstruction and crosstalk of protein-protein interaction networks of Wnt and Hedgehog signaling in Drosophila melanogaster. [FBrf0216393] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...