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Gene Dmel\spn-B

General Information
Symbol	Dmel\spn-B	Species	D. melanogaster
Name	spindle B	Annotation symbol	CG3325
Feature type	protein_coding_gene	FlyBase ID	FBgn0003480
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	spnB
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	88B4-88B4	Sequence location	3R:10,153,773..10,155,330 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene spindle B is referred to in FlyBase by the symbol Dmel\spn-B (CG3325, FBgn0003480). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: recombinase activity. There is experimental evidence that it is involved in the biological process: germarium-derived oocyte fate determination; polarity specification of dorsal/ventral axis; intracellular mRNA localization; oogenesis; polarity specification of anterior/posterior axis; karyosome formation. 16 alleles are reported. The phenotypes of these alleles are annotated with: germline cyst; egg; oocyte; presumptive oocyte; karyosome; oocyte nucleus; dorsal appendage; nurse cell; synaptonemal complex. It has one annotated transcript and one annotated polypeptide. Protein features are: DNA recombination and repair protein Rad51, C-terminal; DNA recombination and repair protein, RecA-like; DNA recombination/repair protein RecA/RadB, ATP-binding domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1630018_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of spn-B. Gene sequence location is 3R:10153773..10155330.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	spn-B: spindle B Maternal-effect lethal. Similar to spn-A but normal eggs also recovered; eggs always unfertilized and abnormal eggs often long.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 88B4-88B4   Limits computationally determined from genome sequence between P{lacW}trxj14A6&P{PZ}trx00347 and P{PZ}cv-c06951
Experimentally Determined Cytological Location
Cytogenetic map Notes References 88B-88B     (Ray, 1998.6.1)
Experimentally Determined Recombination Data
Location	3-54 (Gonzalez-Reyes et al., 1997) 3-52 (Tearle and Nusslein-Volhard, 1987)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\spn-B for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.48 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) spn-B-RA FBtr0082933  NM_057392   1384   341
Additional Transcript Data & Comments
Reported size (kB)	1.35, 0.55 (unknown) (Ghabrial et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein spn-B-PA   FBpp0082392   38.2   341   8.62     NP_476740   AAF55050
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • DNA recombination and repair protein Rad51, C-terminal (IPR013632) DNA recombination and repair protein, RecA-like (IPR016467) P-loop containing nucleoside triphosphate hydrolase (IPR027417) DNA recombination/repair protein RecA/RadB, ATP-binding domain (IPR020588)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF55050   AC016792   AF069531 AAC42663   AY070920 AAL48542   BI163309   BI213930   BI214161   BI640454   BT044133   CO185618   CO282623   CO293630   CO299374   CO313834   CO319274   CZ484477   CZ487601   CZ487602   EC065336   EC089781   EC213883   EC217354   EC222616   EC245466   EC247150   EC262461   GH748388   GH749799   GH751946   GH752740   GH754571   GH755082   GH755821   GH756410   GH757140   GH758380   GH759175   GH770156   GH771813   GH780008   GH782497   GH786565   GH787923   GH788362   GH797325   GH799295   GH800092   GH801974   GH803688   GH808251   GH812754   GH812881   GH819757   GH822487   GH823227   GH826496   GH826521   GH829983   GH830393   GH834318   GH835784   GH836567   GH839867   GH842294   GH843466   GH849791   GH851206   GH854464   GH855137   GH856575   GH857040   GH858799   GH861725   GH863556   GH863727   GH867715   GH868705   GH868993   GH869251   GH869606   GH870527   GH870663   GH871791   GH889658   GH892437   GH893218   GH894373   GH896553   GH896704   GH899320   GH899536   GH901557   GH902703   GH904687   GH906057   GH907474   GH908538   GH910551   GH912654   GH913848   GH918890   GH920802   GH921109   GH921786   GH922343   GH922928   GH925236   GH927398   GH936342   GH937648   GH937753   GH939243   GH939517
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O77135 Q8SZF1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) Comment:rapidly degraded organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   ventral nerve cord primordium   (Fisher et al., 2012) Comment:reported as ventral nerve cord anlage embryonic stage 9 -- 10   ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) oogenesis   organism | ubiquitous   (Ghabrial et al., 1998)
Additional Descriptive Data
	spn-B transcripts are uniformly expressed throughout oogenesis. (Ghabrial et al., 1998)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    26.825 Larval Midgut    12.5 Larval Hindgut    10.1 Larval Malpighian Tubules    16 Larval Fat Body    14.6 Larval Salivary Gland    20 Larval Trachea    28.25 Larval Carcass    18.95 Adult Head    17.8 Adult Eye    14.525 Adult Brain    20.9 Adult Thoracic-Abdominal Ganglion    22.1 Adult Crop    19.1 Adult Midgut    20.3 Adult Hindgut    15.1 Adult Malpighian Tubules    16.1 Adult Fat Body    13.9 Adult Salivary Gland    27.5 Adult Heart    11.725 Adult VirginFemale Spermatheca    14.6 Adult InseminatedFemale Spermatheca    11.5 Adult Ovary    132.2 Adult Testis    15.9 Adult Male Accessory Gland    12.5 Adult Carcass    12.9 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    26.825 Larval Midgut    12.5 Larval Hindgut    10.1 Larval Malpighian Tubules    16 Larval Fat Body    14.6 Larval Salivary Gland    20 Larval Trachea    28.25 Larval Carcass    18.95 Adult Head    17.8 Adult Eye    14.525 Adult Brain    20.9 Adult Thoracic-Abdominal Ganglion    22.1 Adult Crop    19.1 Adult Midgut    20.3 Adult Hindgut    15.1 Adult Malpighian Tubules    16.1 Adult Fat Body    13.9 Adult Salivary Gland    27.5 Adult Heart    11.725 Adult VirginFemale Spermatheca    14.6 Adult InseminatedFemale Spermatheca    11.5 Adult Ovary    132.2 Adult Testis    15.9 Adult Male Accessory Gland    12.5 Adult Carcass    12.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    26.825 Larval Midgut    12.5 Larval Hindgut    10.1 Larval Malpighian Tubules    16 Larval Fat Body    14.6 Larval Salivary Gland    20 Larval Trachea    28.25 Larval Carcass    18.95 Adult Head    17.8 Adult Eye    14.525 Adult Brain    20.9 Adult Thoracic-Abdominal Ganglion    22.1 Adult Crop    19.1 Adult Midgut    20.3 Adult Hindgut    15.1 Adult Malpighian Tubules    16.1 Adult Fat Body    13.9 Adult Salivary Gland    27.5 Adult Heart    11.725 Adult VirginFemale Spermatheca    14.6 Adult InseminatedFemale Spermatheca    11.5 Adult Ovary    132.2 Adult Testis    15.9 Adult Male Accessory Gland    12.5 Adult Carcass    12.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    26.825 Larval Midgut    12.5 Larval Hindgut    10.1 Larval Malpighian Tubules    16 Larval Fat Body    14.6 Larval Salivary Gland    20 Larval Trachea    28.25 Larval Carcass    18.95 Adult Head    17.8 Adult Eye    14.525 Adult Brain    20.9 Adult Thoracic-Abdominal Ganglion    22.1 Adult Crop    19.1 Adult Midgut    20.3 Adult Hindgut    15.1 Adult Malpighian Tubules    16.1 Adult Fat Body    13.9 Adult Salivary Gland    27.5 Adult Heart    11.725 Adult VirginFemale Spermatheca    14.6 Adult InseminatedFemale Spermatheca    11.5 Adult Ovary    132.2 Adult Testis    15.9 Adult Male Accessory Gland    12.5 Adult Carcass    12.9 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    26.825 Larval Midgut    12.5 Larval Hindgut    10.1 Larval Malpighian Tubules    16 Larval Fat Body    14.6 Larval Salivary Gland    20 Larval Trachea    28.25 Larval Carcass    18.95 Adult Head    17.8 Adult Eye    14.525 Adult Brain    20.9 Adult Thoracic-Abdominal Ganglion    22.1 Adult Crop    19.1 Adult Midgut    20.3 Adult Hindgut    15.1 Adult Malpighian Tubules    16.1 Adult Fat Body    13.9 Adult Salivary Gland    27.5 Adult Heart    11.725 Adult VirginFemale Spermatheca    14.6 Adult InseminatedFemale Spermatheca    11.5 Adult Ovary    132.2 Adult Testis    15.9 Adult Male Accessory Gland    12.5 Adult Carcass    12.9 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    26.825 Larval Midgut    12.5 Larval Hindgut    10.1 Larval Malpighian Tubules    16 Larval Fat Body    14.6 Larval Salivary Gland    20 Larval Trachea    28.25 Larval Carcass    18.95 Adult Head    17.8 Adult Eye    14.525 Adult Brain    20.9 Adult Thoracic-Abdominal Ganglion    22.1 Adult Crop    19.1 Adult Midgut    20.3 Adult Hindgut    15.1 Adult Malpighian Tubules    16.1 Adult Fat Body    13.9 Adult Salivary Gland    27.5 Adult Heart    11.725 Adult VirginFemale Spermatheca    14.6 Adult InseminatedFemale Spermatheca    11.5 Adult Ovary    132.2 Adult Testis    15.9 Adult Male Accessory Gland    12.5 Adult Carcass    12.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    26.825 Larval Midgut    12.5 Larval Hindgut    10.1 Larval Malpighian Tubules    16 Larval Fat Body    14.6 Larval Salivary Gland    20 Larval Trachea    28.25 Larval Carcass    18.95 Adult Head    17.8 Adult Eye    14.525 Adult Brain    20.9 Adult Thoracic-Abdominal Ganglion    22.1 Adult Crop    19.1 Adult Midgut    20.3 Adult Hindgut    15.1 Adult Malpighian Tubules    16.1 Adult Fat Body    13.9 Adult Salivary Gland    27.5 Adult Heart    11.725 Adult VirginFemale Spermatheca    14.6 Adult InseminatedFemale Spermatheca    11.5 Adult Ovary    132.2 Adult Testis    15.9 Adult Male Accessory Gland    12.5 Adult Carcass    12.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    26.825 Larval Midgut    12.5 Larval Hindgut    10.1 Larval Malpighian Tubules    16 Larval Fat Body    14.6 Larval Salivary Gland    20 Larval Trachea    28.25 Larval Carcass    18.95 Adult Head    17.8 Adult Eye    14.525 Adult Brain    20.9 Adult Thoracic-Abdominal Ganglion    22.1 Adult Crop    19.1 Adult Midgut    20.3 Adult Hindgut    15.1 Adult Malpighian Tubules    16.1 Adult Fat Body    13.9 Adult Salivary Gland    27.5 Adult Heart    11.725 Adult VirginFemale Spermatheca    14.6 Adult InseminatedFemale Spermatheca    11.5 Adult Ovary    132.2 Adult Testis    15.9 Adult Male Accessory Gland    12.5 Adult Carcass    12.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    17.8   NA  Eye    no informative data   NA  Brain    20.9   26.825  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    22.1   NA  Crop    19.1   no informative data  Midgut    20.3   10.1  Hindgut    15.1   16  Malpighian Tubules    16.1   no informative data  Fat Body    13.9   20  Salivary Gland    no informative data   NA  Heart    no informative data   28.25  Trachea    NA   NA  VirginFemale Spermatheca    14.6   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    132.2   NA  Testis    15.9   NA  Male Accessory Gland    no informative data   18.95  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    2 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    2 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    2 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    2 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    2 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    2 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    2 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    2 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    5 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    2 embryo 14-16hr    3 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    1 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    4 adult female 05day    9 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    5 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    2 embryo 14-16hr    3 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    1 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    4 adult female 05day    9 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    5 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    2 embryo 14-16hr    3 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    1 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    4 adult female 05day    9 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    5 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    2 embryo 14-16hr    3 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    1 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    4 adult female 05day    9 adult female 30day    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    5 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    2 embryo 14-16hr    3 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    1 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    4 adult female 05day    9 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    5 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    2 embryo 14-16hr    3 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    1 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    4 adult female 05day    9 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    5 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    2 embryo 14-16hr    3 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    1 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    4 adult female 05day    9 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    5 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    2 embryo 14-16hr    3 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    1 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    4 adult female 05day    9 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    3 embryonic 1182-4H    2 embryonic GM2    3 embryonic Kc167    4 embryonic S1    1 embryonic S3    3 leg disc CME L1    2 wing disc CME-W2    4 wing disc ML-DmD8    3 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    2 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    2 ovary fGS/OSS    1 ovary OSC    0 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    3 embryonic 1182-4H    2 embryonic GM2    3 embryonic Kc167    4 embryonic S1    1 embryonic S3    3 leg disc CME L1    2 wing disc CME-W2    4 wing disc ML-DmD8    3 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    2 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    2 ovary fGS/OSS    1 ovary OSC    0 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    3 embryonic 1182-4H    2 embryonic GM2    3 embryonic Kc167    4 embryonic S1    1 embryonic S3    3 leg disc CME L1    2 wing disc CME-W2    4 wing disc ML-DmD8    3 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    2 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    2 ovary fGS/OSS    1 ovary OSC    0 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    3 embryonic 1182-4H    2 embryonic GM2    3 embryonic Kc167    4 embryonic S1    1 embryonic S3    3 leg disc CME L1    2 wing disc CME-W2    4 wing disc ML-DmD8    3 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    2 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    2 ovary fGS/OSS    1 ovary OSC    0 ovary OSS    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    3 embryonic 1182-4H    2 embryonic GM2    3 embryonic Kc167    4 embryonic S1    1 embryonic S3    3 leg disc CME L1    2 wing disc CME-W2    4 wing disc ML-DmD8    3 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    2 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    2 ovary fGS/OSS    1 ovary OSC    0 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    3 embryonic 1182-4H    2 embryonic GM2    3 embryonic Kc167    4 embryonic S1    1 embryonic S3    3 leg disc CME L1    2 wing disc CME-W2    4 wing disc ML-DmD8    3 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    2 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    2 ovary fGS/OSS    1 ovary OSC    0 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    3 embryonic 1182-4H    2 embryonic GM2    3 embryonic Kc167    4 embryonic S1    1 embryonic S3    3 leg disc CME L1    2 wing disc CME-W2    4 wing disc ML-DmD8    3 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    2 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    2 ovary fGS/OSS    1 ovary OSC    0 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    3 embryonic 1182-4H    2 embryonic GM2    3 embryonic Kc167    4 embryonic S1    1 embryonic S3    3 leg disc CME L1    2 wing disc CME-W2    4 wing disc ML-DmD8    3 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    2 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    2 ovary fGS/OSS    1 ovary OSC    0 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (Graveley et al., 2011) mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	BDGP expression data • FI03211 RE02671 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003480 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3325-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable spn-B1 spn-B2 spn-BBC spn-BBU spn-BCN viable, with Scer\GAL4pnr-MD237 spn-BGD4235 Sterility Allele female sterile spn-BBU female sterile | recessive spn-BBU Other Phenotypes Allele planar polarity defective spn-BBU radiation sensitive spn-B1 spn-BBU wild-type spn-B+tR144E5.9 spn-B+tRE5.9BE3.5 Phenotype manifest in Allele chorion | maternal effect spn-BBU chorion | maternal effect (with spn-B2) spn-BBU chorion | maternal effect (with spn-BBU) spn-B2 dorsal appendage spn-B1 spn-B2 spn-BBU spn-Bunspecified dorsal appendage | maternal effect spn-BBU dorsal appendage | maternal effect (with spn-BBU) spn-BΔ37C dorsal appendage | maternal effect (with spn-BΔ37C) spn-BBU egg spn-BBU spn-Bunspecified egg | dorsal spn-B1 germline cyst spn-B1 karyosome spn-B1 spn-B2 spn-BBU spn-Bunspecified nuclear membrane & oocyte nucleus spn-BBU nurse cell spn-B1 oocyte spn-B1 oocyte nucleus spn-BBU oocyte nucleus & meiotic cell cycle spn-BBU presumptive oocyte spn-BBU synaptonemal complex spn-B1
Classical Alleles ( 10 )
For All Classical Alleles Show
Allele of spn-B Class Mutagen Stocks Known lesion spn-B1 amorphic allele - genetic evidence ethyl methanesulfonate 2 Yes spn-Bf01980 piggyBac transposase 1 -- spn-Bf05011 piggyBac transposase 1 -- spn-BG6412 P-element activity 1 -- spn-B2 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes spn-BBC antimorphic allele - genetic evidence 0 Yes spn-BBU antimorphic allele - genetic evidence 0 Yes spn-BCN antimorphic allele - genetic evidence 0 Yes spn-Bunspecified 0 -- spn-BΔ37C P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of spn-B Class Mutagen Stocks Known lesion spn-BGD4235 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes spn-BGL01500 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spn-BKK100830 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spn-B+tR144E5.9 in vitro construct - genomic fragment 0 Yes spn-B+tRE5.9BE3.5 in vitro construct - genomic fragment 0 Yes spn-BtR144NS9.5 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6267 (Ryder, 2004.4.26) Df(3R)red3l (Gonzalez-Reyes et al., 1997, Tearle and Nusslein-Volhard, 1987) Df(3R)red-P93 (Ghabrial et al., 1998, Tearle and Nusslein-Volhard, 1987) Df(3R)trxE12 (Ghabrial et al., 1998)
Not disrupted in	Df(3R)red-P52 (Ghabrial et al., 1998, Tearle and Nusslein-Volhard, 1987)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4235} NA P{KK100830} NA P{TRiP.GL01500} NA characterization construct P{R144E5.9} NA P{R144NS9.5} NA P{RE5.9BE3.5} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{?GSV6}spn-BΔ37C NA PBac{WH}spn-Bf01980 NA PBac{WH}spn-Bf05011 NA insertion of mobile activating element P{EP}spn-BG6412 NA
Gene Ontology: Function, Process & Cellular Component ( 18 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
germarium-derived oocyte fate determination inferred from genetic interaction with mus301 (Gonzalez-Reyes et al., 1997) intracellular mRNA localization inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) karyosome formation inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) oogenesis inferred from genetic interaction with fs(1)K10 (Gonzalez-Reyes et al., 1997) polarity specification of anterior/posterior axis inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) polarity specification of dorsal/ventral axis inferred from mutant phenotype (Gonzalez-Reyes et al., 1997)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 14 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR020588 (FlyBase Curators et al., 2004-) DNA binding inferred from electronic annotation with InterPro:IPR020588 (FlyBase Curators et al., 2004-) DNA-dependent ATPase activity inferred from electronic annotation with InterPro:IPR020588 (FlyBase Curators et al., 2004-) recombinase activity inferred from sequence or structural similarity with human XRCC3 (FlyBase, 1992-, Sekelsky et al., 2000)
Biological Process
CV term Evidence References
chromosome condensation traceable author statement (van Eeden and St. Johnston, 1999) DNA recombination inferred from sequence or structural similarity with human XRCC3 (Sekelsky et al., 2000) DNA repair inferred from sequence or structural similarity with human XRCC3 (Sekelsky et al., 2000) non-traceable author statement (van Eeden and St. Johnston, 1999) double-strand break repair traceable author statement (Deng and Lin, 2001, Lee and Orr-Weaver, 2003) intracellular mRNA localization traceable author statement (Cooperstock and Lipshitz, 1997) meiosis traceable author statement (Deng and Lin, 2001) oocyte differentiation non-traceable author statement (Deng and Lin, 2001) oogenesis traceable author statement (van Eeden and St. Johnston, 1999) reciprocal meiotic recombination traceable author statement (van Eeden and St. Johnston, 1999) regulation of translation traceable author statement (Cooperstock and Lipshitz, 1997)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions spn-B allele Gene References spn-B1 spn-D (Gonzalez-Reyes et al., 1997) spn-B2 mei-W68 (Ghabrial and Schupbach, 1999) spn-BBU mei-41 (Ghabrial and Schupbach, 1999) mei-217 (Liu et al., 2000) mei-218 (Liu et al., 2000) mei-P22 (Liu et al., 2002) mei-W68 (Ghabrial and Schupbach, 1999, Liu et al., 2000, Liu et al., 2002) spn-Bunspecified mei-41 (Masrouha et al., 2003) unspecified fs(1)K10 (Gonzalez-Reyes et al., 1997) spn-E (Gonzalez-Reyes et al., 1997)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66822 DroID - A comprehensive database of gene and protein interactions. • FBgn0003480 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41746
Orthologs
OrthoDB Orthologs (33) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG698VKX Dipteran inclusive ortholog search EOG6T1HWB Insect inclusive ortholog search EOG6VHJ44 Arthropod inclusive ortholog search EOG6FTTH9 Metazoa inclusive ortholog search EOG60SC8G
Orthologs in Drosophila Species (EOG698VKX)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\spn-B     Drosophila simulans   Dsim\GD18959 Y   Drosophila sechellia   Dsec\GM24163 Y   Drosophila erecta   Dere\GG16853 Y   Drosophila yakuba   Dyak\GE24233 Y   Drosophila ananassae   Dana\GF17509 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA17378 Y   Drosophila persimilis   Dper\GL23505 Y   Drosophila willistoni   Dwil\GK10943 Y   Drosophila virilis   Dvir\GJ22809 Y   Drosophila mojavensis   Dmoj\GI22807 Y   Drosophila grimshawi   Dgri\GH17480 Y
Orthologs in non-Drosophila Dipterans (EOG6T1HWB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL005399   Anopheles gambiae Malaria mosquito  Agam\AGAP013180   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ008008
Orthologs in non-Dipteran Insects (EOG6VHJ44)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC726326   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG002109   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11845   Atta cephalotes Leafcutter ant  Acep\ACEP26206   Camponotus floridanus Florida carpenter ant  Cflo\CFLO22951   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15943   Linepithema humile Argentine ant  Lhum\LH17440   Pogonomyrmex barbatus Red harvester ant  Pbar\PB12817   Acyrthosiphon pisum Pea aphid  Apim\ACYPI003584   Tribolium castaneum Red flour beetle  Tcas\spn-B
Orthologs in non-Insect Arthropods (EOG6FTTH9)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW021580
Orthologs in non-Arthropod Metazoa (EOG60SC8G)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_023836gn   Danio rerio Zebrafish  Drer\xrcc3   Xenopus tropicalis Western clawed frog  Xtro\xrcc3   Gallus gallus Domestic chicken  Ggal\XRCC3   Mus musculus House mouse  Mmus\Xrcc3   Rattus norvegicus Norway rat  Rnor\LOC100359601   Homo sapiens Human  Hsap\XRCC3
Human Orthologs (1)
Gene OMIM HGNC Hsap\XRCC3
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD18959 Drosophila sechellia Dsec\GM24163 Drosophila erecta Dere\GG16853 Drosophila yakuba Dyak\GE24233 Drosophila ananassae Dana\GF17509 Drosophila pseudoobscura pseudoobscura Dpse\GA17378 Drosophila persimilis Dper\GL23505 Drosophila willistoni Dwil\GK10943 Drosophila virilis Dvir\GJ22809 Drosophila mojavensis Dmoj\GI22807 Drosophila grimshawi Dgri\GH17480
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	33271 w1118; P{EP}spn-BG6412 43158 y1 v1; P{TRiP.GL01500}attP2 3324 Dp(1;Y)BS, B+; ru1 st1 spn-B1 e1 ca1/TM3, Sb1
Harvard	- PBac{WH}spn-Bf01980 - PBac{WH}spn-Bf05011
Kyoto	106978 Dp(1;Y)BS; ru1 st1 spn-B1 e1 ca1/TM3, Sb1
VDRC	v105623 P{KK100830}VIE-260B v30490 w1118; P{GD4235}v30490 v30491 w1118; P{GD4235}v30491
Genomic Clones ( 1 )
	BACR26A02 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 106 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE02671
Other clones	FI03211
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE19578 RE19883 SD23317
Other clones	45151 47370 73501 105557 108113 173278 569473 CK01.7C.A6 EK010527 EK160845 EK175163 EK203903 EK234157 EK277530 RP003003176 RP003007305 RP003019450 RP003044610 RP003057210 RP003063099 RP003063257 RP003067086 RP003068433 RP003071539 RP003084383 RP003087009 RP003097233 RP003098483 RP003112359 RP003123902 RP003126311 RP003127631 RP003127769 RP003132165 RP003138146 RP003152425 RP003154723 RP003161392 RP003172607 RP003186857 RP003197044 RP003203515 RP003212227 RP003217256 RP003224922 RP003233344 RP003246511 RP003247828 RP003249129 RP003253120 RP003258962 RP003264286 RP003269870 RP003271207 RP003274404 RP003277174 RP003278000 RP003284979 RP003286621 RP003299540 RP003304659 RP003306333 RP003306682 RP003309852 RP003317566 RP003318879 RP003324112 RP003324431 RP003332999 RP003353791 RP003356369 RP003357175 RP003365880 RP003380839 RP003407819 RP003421707 RP003428339 RP003440938 RP003455411 RP003468662 RP003472360 RP003511340 RP003545928 RP003554698 RP003563480 RP003566711 RP003572450 RP003576478 RP003585032 RP003593561 RP003601390 RP003612942 RP003613836 RP003628762 RP003630345 RP003635705 RP003650559 RP003661645 RP003662056 RP003675393 RP003681602
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003480 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41746
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Analysis of mutants of the 5 spindle genes (spn-A, spn-B, mus301, spn-D and spn-E) reveals that the group of genes is required for each of the symmetry-breaking steps that generate polarity during axis formation. spn-E is required for the localisation of fs(1)K10, bcd and osk mRNAs, spn-A. spn-B, mus301 and spn-D play an indirect role in this process. Double mutants analysis suggests that spn-B and spn-D genes play a partially redundant role in oocyte positioning. (Gonzalez-Reyes et al., 1997) Mutations in the spn-A, spn-B and spn-D genes result in a very similar phenotype to those in mus301, and in all cases the misplacement of the oocyte has the same consequences on the establishment of AP polarity in the egg chamber, as determined by the slbo expression in the follicle cells and the localization of osk and bcd mRNAs within the oocyte. (Gonzalez-Reyes and St. Johnston, 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057392 RefSeq (Proteins) • NP_476740 DDBJ / EMBL / GenBank • AAC42663 AAF55050 AAL48542 AC016792 AF069531 AY070920 BI163309 CZ484477 CZ487601 CZ487602 Entrez Gene - A searchable database of RefSeq genes. • 41746 UniProtKB/TrEMBL • O77135 Q8SZF1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • DNA recombination and repair protein Rad51, C-terminal (IPR013632) DNA recombination and repair protein, RecA-like (IPR016467) P-loop containing nucleoside triphosphate hydrolase (IPR027417) DNA recombination/repair protein RecA/RadB, ATP-binding domain (IPR020588) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • FI03211 RE02671
Linkouts
	BioGRID - A database of protein and genetic interactions • 66822 DroID - A comprehensive database of gene and protein interactions. • FBgn0003480 DRSC - Results from RNAi screens. • FBgn0003480 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003480 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3325-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003480 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41746 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41746 modMine - Data generated by the modENCODE project. • FBgn0003480
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	CG3325 (Keleman et al., 2009.8.5, Ni et al., 2010.12.1) Rad51 (Jang et al., 2003) rad51-like (Hochwagen, 2006) spnB (Deng and Lin, 2001, Masrouha et al., 2003, Liu et al., 2002, McKim et al., 2002, Perelmuter et al., 2002, Johnstone and Lasko, 2001, McKee et al., 2000, Huynh and St. Johnston, 2000, McKim et al., 2000, Liu et al., 2000, Liu and McKim, 2000, Morris et al., 2000, Thacker, 1999, Ghabrial and Schupbach, 1999, Ghabrial et al., 1998, Ghabrial et al., 1998, Ray et al., 1996, Hochwagen, 2006, Wei and Rong, 2007, Jang et al., 2003, Navarro et al., 2009, Lancaster et al., 2010, Lake et al., 2011) SpnB (Lipshitz and Smibert, 2000) spn-B (Mukai et al., 2006, McCaffrey et al., 2006, Mehrotra and McKim, 2006, Abdu et al., 2007, Joyce and McKim, 2009, Anderson et al., 2009, Joyce et al., 2009, Reis et al., 2011, Ferguson et al., 2012) XRCC3 (Abdu et al., 2007)
Name Synonym	Spindle-B (Berg, 2005) spindle-B (Lee and Orr-Weaver, 2003, Riechmann and Ephrussi, 2001) spindle B (Mukai et al., 2006, Abdu et al., 2007) Spindle B (Felix et al., 2012)
Secondary FlyBase IDs
References ( 76 )
Generate a list of	All references relating to this gene ( 76 )
List References by type	Research paper  ( 32 ) Supplementary material  ( 3 ) Review  ( 15 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 9 ) FlyBase analysis  ( 2 ) Stock list  ( 1 ) DNA/RNA sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 ) Book  ( 1 )
Recent research papers ( 5 )
	Felix et al., 2012, Genetics 191(3): 989--1002 Age-Specific Variation in Immune Response in Drosophila melanogaster Has a Genetic Basis. [FBrf0218944] Ferguson et al., 2012, J. Cell Sci. 125(6): 1407--1419 Modulation of gurken translation by insulin and TOR signaling in Drosophila. [FBrf0218097] Joyce et al., 2012, Genetics 191(3): 739--746 Multiple barriers to nonhomologous DNA end joining during meiosis in Drosophila. [FBrf0218878] Lake et al., 2011, PLoS Genet. 7(2): e1002005 The Drosophila zinc finger protein trade embargo is required for double strand break formation in meiosis. [FBrf0213142] Reis et al., 2011, PLoS ONE 6(3): e17512 Drosophila Genes That Affect Meiosis Duration Are among the Meiosis Related Genes That Are More Often Found Duplicated. [FBrf0213266] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...