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Gene Dmel\spn-E

General Information
Symbol	Dmel\spn-E	Species	D. melanogaster
Name	spindle E	Annotation symbol	CG3158
Feature type	protein_coding_gene	FlyBase ID	FBgn0003483
Gene Model Status	Current	Stock availability	14 publicly available
Also Known As	hls, spnE
Genomic Location
Chromosome (arm)	3R	Recombination map	3-62
Cytogenetic map	89A5-89A5	Sequence location	3R:11,663,159..11,670,138 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene spindle E is referred to in FlyBase by the symbol Dmel\spn-E (CG3158, FBgn0003483). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: helicase activity; ATP-dependent RNA helicase activity. There is experimental evidence for 16 unique biological process terms, many of which group under: cellular component organization or biogenesis; biological regulation; single-organism developmental process; localization; multicellular organism reproduction; anterior/posterior axis specification; gene silencing; dorsal/ventral axis specification; germarium-derived oocyte differentiation; negative regulation of cellular biosynthetic process; regulation of developmental process; karyosome formation; positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; cell cycle; polarity specification of dorsal/ventral axis. 35 alleles are reported. The phenotypes of these alleles are annotated with: germline cyst; P granule; oocyte; karyosome; spermatocyte; egg chamber; microtubule; dorsal appendage; synaptonemal complex. It has one annotated transcript and one annotated polypeptide. Protein features are: DNA/RNA helicase, DEAD/DEAH box type, N-terminal; Helicase, C-terminal; Helicase, superfamily 1/2, ATP-binding domain; Helicase-associated domain; Tudor domain; Zinc finger, C2H2; Zinc finger, C2H2-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1636886_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of spn-E. Gene sequence location is 3R:11663159..11670138.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	spn-E: spindle E Maternal-effect lethal. Similar to spn-A.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02	Controlled Vocabulary Terms negative regulation of transposition
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 89A5-89A5   Limits computationally determined from genome sequence between P{PZ}l(3)0653606536 and P{PZ}blp01618
Experimentally Determined Cytological Location
Cytogenetic map Notes References 89B1-89B2   (determined by in situ hybridisation)   (Spradling et al., 1999) 89A5-89A6   (determined by in situ hybridisation)   (Gillespie and Berg, 1995) 89A5-89A6   (determined by in situ hybridisation)   (Berg, 1994) 89B1-89B2     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	3-62 (Tearle and Nusslein-Volhard, 1987, Gonzalez-Reyes et al., 1997)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\spn-E for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) spn-E-RA FBtr0083183  NM_057393   4661   1434
Additional Transcript Data & Comments
Reported size (kB)	4.6 (northern blot) (Gillespie and Berg, 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein spn-E-PA   FBpp0082637   164.5   1434   7.22     NP_476741   AAF55235
Additional Polypeptide Data & Comments
Reported size (kDa)	1441 (aa); 155 (kD predicted) (Gillespie and Berg, 1995)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, C2H2-like (IPR015880) Helicase, C-terminal (IPR001650) Tudor domain (IPR002999) Zinc finger, C2H2 (IPR007087) Helicase-associated domain (IPR007502) Helicase, superfamily 1/2, ATP-binding domain (IPR014001) P-loop containing nucleoside triphosphate hydrolase (IPR027417) DNA/RNA helicase, DEAD/DEAH box type, N-terminal (IPR011545)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA142297   AE014297 AAF55235   AC010122   AQ025574   BP559549   BP559550   BT029921   BT100306   CO267601   CZ466715   CZ471194   CZ481218   CZ481219   CZ481860   EC083913   EC084674   EL871700   EL871701   EL871823   EL871824   EL871953   EL871954   GH943009   GH943732   GH943896   GH944462   GH944854   GH947757   GH949818   GH949987   GH950044   GH950511   GH950896   GH954075   GH954899   GH955531   GH958151   GH960212   GH961164   GH964422   GH969215   GH970178   GH972678   GH973298   GH978641   S79915 AAB35476
UniProtKB/Swiss-Prot	Q9VF26
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage | early   organism | ubiquitous   (Gillespie and Berg, 1995) adult stage & oogenesis   germarium   (Gillespie and Berg, 1995) female germline stem cell   (Gillespie and Berg, 1995) nurse cell   (Gillespie and Berg, 1995) oogenesis & adult stage | female   ovary   (Gillespie and Berg, 1995)
Additional Descriptive Data
	spn-E transcripts are expressed predominantly in ovaries but small amounts are detected in total male and female carcass RNA. In the germarium, expression is limited to the germline cells. Expression decreases through oogenesis stages S4/S5, increases during S8 and peaks in nurse cells at stage S10. No transcript is detected in the oocyte until stage S11 when it is deposited from the nurse cells. spn-E transcripts are uniformly distributed in early embryos. (Gillespie and Berg, 1995)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Grimaud et al., 2006) nucleus inferred from direct assay (Grimaud et al., 2006) P granule inferred from direct assay (Patil and Kai, 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland    13.3 Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland    14.1 Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland    13.2 Adult Carcass    17.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland    13.3 Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland    14.1 Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland    13.2 Adult Carcass    17.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland    13.3 Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland    14.1 Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland    13.2 Adult Carcass    17.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland    13.3 Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland    14.1 Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland    13.2 Adult Carcass    17.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland    13.3 Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland    14.1 Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland    13.2 Adult Carcass    17.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland    13.3 Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland    14.1 Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland    13.2 Adult Carcass    17.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland    13.3 Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland    14.1 Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland    13.2 Adult Carcass    17.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland    13.3 Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland    14.1 Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland    13.2 Adult Carcass    17.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    10.1   NA  Eye    33.575   NA  Brain    4.4   49.15  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    5.7   NA  Crop    5.9   8.2  Midgut    8.1   8.9  Hindgut    5.3   5.3  Malpighian Tubules    5.4   53.5  Fat Body    11.2   no informative data  Salivary Gland    no informative data   NA  Heart    6.525   12.15  Trachea    NA   NA  VirginFemale Spermatheca    6.5   NA  InseminatedFemale Spermatheca    8.6   NA  Ovary    466.7   NA  Testis    66.6   NA  Male Accessory Gland    no informative data   12.7  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    52 ovary, mated 4-day female    35 testis, mated 4-day male    3 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female  (52) ovary, mated 4-day female  (35) testis, mated 4-day male    3 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    52 ovary, mated 4-day female    35 testis, mated 4-day male    3 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    52 ovary, mated 4-day female    35 testis, mated 4-day male    3 accessory gland, mated 4-day male    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    52 ovary, mated 4-day female    35 testis, mated 4-day male    3 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female  (52) ovary, mated 4-day female  (35) testis, mated 4-day male    3 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    52 ovary, mated 4-day female    35 testis, mated 4-day male    3 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    52 ovary, mated 4-day female    35 testis, mated 4-day male    3 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    2 embryo 08-10hr    2 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    13 adult female 05day    25 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (39) embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    2 embryo 08-10hr    2 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    13 adult female 05day    25 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    2 embryo 08-10hr    2 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    13 adult female 05day    25 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    2 embryo 08-10hr    2 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    13 adult female 05day    25 adult female 30day    23 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    2 embryo 08-10hr    2 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    13 adult female 05day    25 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (39) embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    2 embryo 08-10hr    2 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    13 adult female 05day    25 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    2 embryo 08-10hr    2 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    13 adult female 05day    25 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    2 embryo 08-10hr    2 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    2 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    2 adult female 01day    13 adult female 05day    25 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    10 embryonic 1182-4H    7 embryonic GM2    12 embryonic Kc167    20 embryonic S1    26 embryonic S3    7 leg disc CME L1    11 wing disc CME-W2    26 wing disc ML-DmD8    9 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    4 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    16 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    78 ovary OSC    48 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    10 embryonic 1182-4H    7 embryonic GM2    12 embryonic Kc167    20 embryonic S1  26 embryonic S3    7 leg disc CME L1    11 wing disc CME-W2  26 wing disc ML-DmD8    9 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    4 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    16 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS  (78) ovary OSC  (48) ovary OSS  (28) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    10 embryonic 1182-4H    7 embryonic GM2    12 embryonic Kc167    20 embryonic S1    26 embryonic S3    7 leg disc CME L1    11 wing disc CME-W2    26 wing disc ML-DmD8    9 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    4 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    16 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    78 ovary OSC    48 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    10 embryonic 1182-4H    7 embryonic GM2    12 embryonic Kc167    20 embryonic S1    26 embryonic S3    7 leg disc CME L1    11 wing disc CME-W2    26 wing disc ML-DmD8    9 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    4 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    16 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    78 ovary OSC    48 ovary OSS    28 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    10 embryonic 1182-4H    7 embryonic GM2    12 embryonic Kc167    20 embryonic S1    26 embryonic S3    7 leg disc CME L1    11 wing disc CME-W2    26 wing disc ML-DmD8    9 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    4 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    16 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    78 ovary OSC    48 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    10 embryonic 1182-4H    7 embryonic GM2    12 embryonic Kc167    20 embryonic S1  26 embryonic S3    7 leg disc CME L1    11 wing disc CME-W2  26 wing disc ML-DmD8    9 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    4 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    16 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS  (78) ovary OSC  (48) ovary OSS  28 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    10 embryonic 1182-4H    7 embryonic GM2    12 embryonic Kc167    20 embryonic S1    26 embryonic S3    7 leg disc CME L1    11 wing disc CME-W2    26 wing disc ML-DmD8    9 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    4 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    16 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    78 ovary OSC    48 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    7 Schneider line 2 Sg4    10 embryonic 1182-4H    7 embryonic GM2    12 embryonic Kc167    20 embryonic S1    26 embryonic S3    7 leg disc CME L1    11 wing disc CME-W2    26 wing disc ML-DmD8    9 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    12 wing disc ML-DmD21    4 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    16 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    14 tumorous blood cells mbn2    15 ovary fGS/OSS    78 ovary OSC    48 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3  (29) Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3  29 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_13 mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	BDGP expression data • PC00432 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003483 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3158-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | recessive spn-Ehls-Δ125 lethal | polyphasic | recessive spn-Ehls-Δ139 spn-Ehls-Δ157 lethal | recessive spn-Ehls-Δ125 spn-Ehls-Δ157 viable, with Scer\GAL4pnr-MD237 spn-EGD10299 Sterility Allele female sterile spn-E167 spn-E167R33 spn-Ehls-03987 female sterile, with Scer\GAL4nos.PG, Scer\GAL4nos.UTR.T:Hsim\VP16, Scer\GAL4otu.T:Hsim\VP16 spn-EdsRNA.shRNA.Scer\UAS.P\T female sterile | recessive spn-Ehls-03987 female sterile germline-dependent, with Scer\GAL4nos.PG, Scer\GAL4nos.UTR.T:Hsim\VP16, Scer\GAL4otu.T:Hsim\VP16 spn-EdsRNA.shRNA.Scer\UAS.P\T fertile (with spn-E1) spn-Ehls-Δ125 fertile (with spn-Ehls-Δ125) spn-E1 male fertile spn-E167 spn-E167R33 male sterile (with spn-E1) spn-Ehls-Δ125 male sterile (with spn-Ehls-Δ125) spn-E1 male sterile | partially spn-E1 sterile (with spn-Ehls-03987) spn-Ehls-Δ139 spn-Ehls-Δ157 sterile (with spn-Ehls-Δ139) spn-Ehls-03987 sterile (with spn-Ehls-Δ157) spn-Ehls-03987 Other Phenotypes Allele meiotic cell cycle defective spn-E1 spn-E2 spn-Ehls-03987 meiotic cell cycle defective (with spn-E1) spn-E2 spn-Ehls-03987 spn-Ehls-Δ125 meiotic cell cycle defective (with spn-E2) spn-E1 meiotic cell cycle defective (with spn-Ehls-03987) spn-E1 meiotic cell cycle defective (with spn-Ehls-Δ125) spn-E1 suppressor of variegation spn-E1 spn-EDE8 spn-Ehls-03987 spn-Ehls-E1 spn-Ehls-Δ125 suppressor of variegation, with P{T1(-FRT)}T190-177 spn-Ehls-Δ125 suppressor of variegation, with P{T1}T190-177 spn-Ehls-Δ125 Phenotype manifest in Allele dorsal appendage spn-E1 spn-E167 spn-E167R33 spn-Ehls-03987 egg | dorsal spn-E1 egg chamber spn-E167R33 spn-Ehls-03987 egg chamber | germline clone spn-Eunspecified germline cyst spn-E1 karyosome spn-E1 spn-Eunspecified meiosis & nuclear chromosome spn-E1 spn-E2 spn-Ehls-03987 meiosis & nuclear chromosome (with spn-E1) spn-E2 spn-Ehls-Δ125 spn-Ehls-03987 meiosis & nuclear chromosome (with spn-E2) spn-E1 meiosis & nuclear chromosome (with spn-Ehls-03987) spn-E1 meiosis & nuclear chromosome (with spn-Ehls-Δ125) spn-E1 meiosis & nuclear chromosome | male spn-E1 microtubule spn-Ehls-03987 oocyte spn-E1 spn-Ehls-03987 oocyte (with spn-E4E2-14) spn-Ehls-Δ157 oocyte (with spn-Ehls-Δ157) spn-E4E2-14 P granule (with spn-E1) spn-Ehls-03987 P granule (with spn-Ehls-03987) spn-E1 spermatocyte spn-E1 synaptonemal complex spn-E1
Classical Alleles ( 25 )
For All Classical Alleles Show
Allele of spn-E Class Mutagen Stocks Known lesion spn-E1 hypomorphic allele - genetic evidence ethyl methanesulfonate 2 -- spn-Ec00786 piggyBac transposase 2 -- spn-Ec07043 piggyBac transposase 1 -- spn-Ee03441 piggyBac transposase 1 -- spn-Ehls-03987 hypomorphic allele - genetic evidence P-element activity 1 Yes spn-Ehls-Δ125 loss of function allele Delta2-3 1 Yes spn-E167R33 amorphic allele - genetic evidence Delta2-3 0 -- spn-E100.37 0 -- spn-E125 0 -- spn-E167 hypomorphic allele - genetic evidence Delta2-3 0 -- spn-E167R18 Delta2-3 0 -- spn-E2 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- spn-E2A9-14 ethyl methanesulfonate 0 -- spn-E4E2-14 ethyl methanesulfonate 0 -- spn-E7G2-5 ethyl methanesulfonate 0 -- spn-E8D4-11 ethyl methanesulfonate 0 -- spn-E9A2-17 ethyl methanesulfonate 0 -- spn-E9A9-18 ethyl methanesulfonate 0 -- spn-ED58 0 -- spn-EDE8 0 -- spn-Ee04103 piggyBac transposase 0 -- spn-Ehls-E1 0 -- spn-Ehls-Δ139 Delta2-3 0 Yes spn-Ehls-Δ157 loss of function allele Delta2-3 0 Yes spn-Eunspecified 0 --
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of spn-E Class Mutagen Stocks Known lesion spn-EGD10299 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes spn-EGL00206 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spn-EHMS00117 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spn-EJF01405 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spn-EKK101525 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spn-EdsRNA.cDa in vitro construct - RNAi 0 Yes spn-EdsRNA.shRNA.Scer\UAS.P\T in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes spn-ENIG.3158R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes spn-ET:Uuuu\T7 in vitro construct - coding region fusion 0 Yes spn-EtCH322-93D04 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)160R34 (Matsubayashi and Yamamoto, 1998) Df(3R)160R104 (Matsubayashi and Yamamoto, 1998) Df(3R)167R11 (Matsubayashi and Yamamoto, 1998) Df(3R)167R17 (Matsubayashi and Yamamoto, 1998) Df(3R)167R37 (Matsubayashi and Yamamoto, 1998) Df(3R)c(3)G-2 (Matsubayashi and Yamamoto, 1998) Df(3R)Exel6175 (Ryder, 2004.4.26) Df(3R)Exel8162 (Ryder, 2004.4.26) Df(3R)Po2 (Matsubayashi and Yamamoto, 1998) Df(3R)R24 (Matsubayashi and Yamamoto, 1998) Df(3R)R44 (Matsubayashi and Yamamoto, 1998) Df(3R)sbd105 (Gonzalez-Reyes et al., 1997, Gillespie and Berg, 1995)
Not disrupted in	Df(3R)160R5 (Matsubayashi and Yamamoto, 1998) Df(3R)160R8 (Matsubayashi and Yamamoto, 1998) Df(3R)160R32 (Matsubayashi and Yamamoto, 1998)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10299} NA P{KK101525} NA P{NIG.3158R} NA P{TRiP.GL00206} NA P{TRiP.HMS00117} NA P{TRiP.JF01405} NA P{UASp-spn-E.shRNA} NA characterization construct PBac{CH322-93D04} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}spn-Ec00786 NA PBac{PB}spn-Ec07043 NA PBac{RB}spn-Ee03441 NA PBac{RB}spn-Ee04103 NA insertion of enhancer trap P{lwB}spn-E167 No P{lwB}spn-E167R18 No P{lwB}spn-E167R33 No P{PZ}spn-Ehls-03987 No
Gene Ontology: Function, Process & Cellular Component ( 30 unique terms )
Terms Based on Experimental Evidence ( 19 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
chromatin silencing inferred from mutant phenotype (Pal-Bhadra et al., 2004) dorsal appendage formation inferred from mutant phenotype (Klattenhoff et al., 2007) germarium-derived oocyte fate determination inferred from genetic interaction with mus301 (Gonzalez-Reyes et al., 1997) intracellular mRNA localization inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) karyosome formation inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) mitotic chromosome condensation inferred from mutant phenotype (Pek and Kai, 2011) negative regulation of transposition inferred from mutant phenotype (Specchia et al., 2010) oocyte localization involved in germarium-derived egg chamber formation inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) oocyte maturation inferred from mutant phenotype (Kennerdell et al., 2002) oogenesis inferred from genetic interaction with fs(1)K10 (Gonzalez-Reyes et al., 1997) polarity specification of anterior/posterior axis inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) polarity specification of dorsal/ventral axis inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) pole plasm oskar mRNA localization inferred from mutant phenotype (Martin et al., 2003) positive regulation of nuclear-transcribed mRNA poly(A) tail shortening inferred from mutant phenotype (Rouget et al., 2010) regulation of pole plasm oskar mRNA localization inferred from mutant phenotype (Cook et al., 2004) targeting of mRNA for destruction involved in RNA interference inferred from mutant phenotype (Kennerdell et al., 2002)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Grimaud et al., 2006) nucleus inferred from direct assay (Grimaud et al., 2006) P granule inferred from direct assay (Patil and Kai, 2010)
Terms Based on Predictions or Assertions ( 14 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR001650, InterPro:IPR011545 (FlyBase Curators et al., 2004-) ATP-dependent RNA helicase activity inferred from sequence or structural similarity with InterPro:IPR001410 (FlyBase, 1992-) helicase activity inferred from sequence or structural similarity (Lasko, 2000) nucleic acid binding inferred from electronic annotation with InterPro:IPR001650, InterPro:IPR011545 (FlyBase Curators et al., 2004-) RNA-dependent ATPase activity traceable author statement (Wylie, 1999) RNA helicase activity traceable author statement (van Eeden and St. Johnston, 1999) zinc ion binding inferred from electronic annotation with InterPro:IPR007087, InterPro:IPR015880 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
intracellular mRNA localization traceable author statement (Cooperstock and Lipshitz, 1997) oocyte differentiation non-traceable author statement (Deng and Lin, 2001) oogenesis traceable author statement (Wylie, 1999, van Eeden and St. Johnston, 1999) pole plasm assembly non-traceable author statement (Williamson and Lehmann, 1996) RNA interference traceable author statement (Lavorgna et al., 2004, Murchison and Hannon, 2004) RNA localization traceable author statement (Wylie, 1999)
Cellular Component
CV term Evidence References
cytoplasm non-traceable author statement (Morris and Lehmann, 1999)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
spn-E - qin anti tag coimmunoprecipitation, anti tag western blot, western blot (Anand and Kai, 2012) spn-E - tej anti tag coimmunoprecipitation, anti tag western blot (Patil and Kai, 2010)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions spn-E allele Gene References spn-E1 spn-D (Gonzalez-Reyes et al., 1997) Ste (Stapleton et al., 2001) spn-Ehls-Δ125 Ste (Stapleton et al., 2001) unspecified spn-B (Gonzalez-Reyes et al., 1997)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66977 DroID - A comprehensive database of gene and protein interactions. • FBgn0003483 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41919
Orthologs
OrthoDB Orthologs (44) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6C2HVT Dipteran inclusive ortholog search EOG6N04NW Insect inclusive ortholog search EOG6BK3ZQ Arthropod inclusive ortholog search EOG6BG79T Metazoa inclusive ortholog search EOG6ZPSN0
Orthologs in Drosophila Species (EOG6C2HVT)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\spn-E     Drosophila simulans   Dsim\GD19053 Y   Drosophila sechellia   Dsec\GM24265 Y   Drosophila erecta   Dere\GG16958 Y   Drosophila yakuba   Dyak\GE24344 Y   Drosophila ananassae   Dana\GF18547 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA16329 Y   Drosophila persimilis   Dper\GL22075 Y   Drosophila willistoni   Dwil\GK11214 Y   Drosophila virilis   Dvir\GJ23765 Y   Drosophila mojavensis   Dmoj\GI22949 Y   Drosophila grimshawi   Dgri\GH23451 Y
Orthologs in non-Drosophila Dipterans (EOG6N04NW)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\spn-E   Anopheles gambiae Malaria mosquito  Agam\AGAP002829   Culex quinquefasciatus Southern house mosquito  Cqui\spn-E
Orthologs in non-Dipteran Insects (EOG6BK3ZQ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\spn-E   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG002966   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH18136   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH15893   Atta cephalotes Leafcutter ant  Acep\ACEP10965   Atta cephalotes Leafcutter ant  Acep\ACEP15960   Camponotus floridanus Florida carpenter ant  Cflo\CFLO13379   Camponotus floridanus Florida carpenter ant  Cflo\CFLO22023   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL20859   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL13977   Linepithema humile Argentine ant  Lhum\LH23051   Linepithema humile Argentine ant  Lhum\LH14588   Pogonomyrmex barbatus Red harvester ant  Pbar\PB11696   Pogonomyrmex barbatus Red harvester ant  Pbar\CG:02545989   Solenopsis invicta Red fire ant  Sinv\SINV11082   Solenopsis invicta Red fire ant  Sinv\SINV14885   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005239   Bombyx mori Silkmoth  Bmor\BGIBMGA004949   Pediculus humanus Human body louse  Phum\PHUM492140   Tribolium castaneum Red flour beetle  Tcas\spn-E
Orthologs in non-Insect Arthropods (EOG6BG79T)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_316598
Orthologs in non-Arthropod Metazoa (EOG6ZPSN0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_026759gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_002501gn   Danio rerio Zebrafish  Drer\tdrd9   Xenopus tropicalis Western clawed frog  Xtro\tdrd9   Gallus gallus Domestic chicken  Ggal\TDRD9   Mus musculus House mouse  Mmus\Tdrd9   Rattus norvegicus Norway rat  Rnor\Tdrd9   Homo sapiens Human  Hsap\TDRD9
Human Orthologs (1)
Gene OMIM HGNC Hsap\TDRD9
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD19053 Drosophila sechellia Dsec\GM24265 Drosophila erecta Dere\GG16958 Drosophila yakuba Dyak\GE24344 Drosophila ananassae Dana\GF18547 Drosophila pseudoobscura pseudoobscura Dpse\GA16329 Drosophila persimilis Dper\GL22075 Drosophila willistoni Dwil\GK11214 Drosophila virilis Dvir\GJ23765 Drosophila mojavensis Dmoj\GI22949 Drosophila grimshawi Dgri\GH23451
Stocks & Reagents
Stocks Listed in FlyBase ( 14 )
Bloomington	31620 y1 v1; P{TRiP.JF01405}attP2 35303 y1 sc* v1; P{TRiP.GL00206}attP2 34808 y1 sc* v1; P{TRiP.HMS00117}attP2 10274 w1118; PBac{PB}spn-Ec00786/TM6B, Tb1 43638 P{neoFRT}82B ry506 spn-Ehls-Δ125 e1/TM3, ry* Sb1 3327 ru1 st1 spn-E1 e1 ca1/TM3, Sb1 Ser1
Harvard	- PBac{PB}spn-Ec00786 - PBac{PB}spn-Ec07043 - PBac{RB}spn-Ee03441
VDRC	v103913 P{KK101525}VIE-260B v21376 w1118; P{GD10299}v21376 v21374 w1118; P{GD10299}v21374/TM3
	More stocks available...
Genomic Clones ( 1 )
	BACR06B08 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 33 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones	FI13017 IP03663
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	31122 38618 CK01654 EK151039 FGM321E10 IP03763 IP03863 IP03963 RP004042001 RP004094296 RP004106336 RP004120062 RP004164037 RP004171435 RP004177803 RP004178554 RP004180611 RP004205358 RP004237204 RP004240366 RP004246114 RP004248147 RP004252132 RP004257601 RP004384232 RP004394487 RP004430887 RP004444415 RP004504120 RP004537884 RP004547222
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003483 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41919
Antibody Information
	polyclonal (Patil and Kai, 2010)
Other Information
Discoverer
Etymology
	Named "homeless" based on oocyte mislocalisation phenotype and RNA localisation phenotype. (Gillespie and Berg, 1995)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: spn-E CG3158 (Kennison, 1999.11)
Source for database merge of
Additional comments
Other Comments
	spn-E is required for the accumulation of repeat-associated small interfering RNAs (rasiRNAs). (Vagin et al., 2006) piwi, aub and spn-E are suppressors of repeat-induced silencing, as seen at heterochromatic tandem arrays of insertions of the P{lacW} transposon. (Pal-Bhadra et al., 2004) spn-E mutations have a dramatic effect on the distribution of Su(var)205 protein. Instead of the Su(var)205 protein being localised to pericentric heterochromatin and the fourth chromosome it is distributed across the whole of the polytene chromosomes. There is a reduction in the levels of the methyl-K9 form of His3. (Pal-Bhadra et al., 2004) Identification: in a germline clone screen for mutants that are defective in localisation of an Avic\GFP-stau marker in living oocytes. 6 alleles of spn-E have been identified in the screen. (Martin et al., 2003) spn-E mutants block RNAi activation which normally occurs during egg maturation. (Kennerdell et al., 2002) spn-E has a role in repressing the Ste locus and may have a role in repressing other repeated sequences. (Stapleton et al., 2001) Analysis of mutants of the 5 spindle genes (spn-A, spn-B, mus301, spn-D and spn-E) reveals that the group of genes is required for each of the symmetry-breaking steps that generate polarity during axis formation. spn-E is required for the localisation of fs(1)K10, bcd and osk mRNAs, spn-A. spn-B, mus301 and spn-D play an indirect role in this process. (Gonzalez-Reyes et al., 1997) spn-E gene product is necessary for the correct localisation of several RNAs that are involved in anteroposterior and dorsoventral axis formation during oogenesis. (Gillespie and Berg, 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057393 RefSeq (Proteins) • NP_476741 DDBJ / EMBL / GenBank • AAB35476 AAF55235 AC010122 AQ025574 CZ466715 CZ471194 CZ481218 CZ481219 CZ481860 S79915 Entrez Gene - A searchable database of RefSeq genes. • 41919 UniProtKB/Swiss-Prot • Q9VF26
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, C2H2-like (IPR015880) Helicase, C-terminal (IPR001650) Tudor domain (IPR002999) Zinc finger, C2H2 (IPR007087) Helicase-associated domain (IPR007502) Helicase, superfamily 1/2, ATP-binding domain (IPR014001) P-loop containing nucleoside triphosphate hydrolase (IPR027417) DNA/RNA helicase, DEAD/DEAH box type, N-terminal (IPR011545) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • PC00432
Linkouts
	BioGRID - A database of protein and genetic interactions • 66977 DroID - A comprehensive database of gene and protein interactions. • FBgn0003483 DRSC - Results from RNAi screens. • FBgn0003483 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003483 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3158-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003483 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41919 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/homels1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41919 modMine - Data generated by the modENCODE project. • FBgn0003483
Synonyms & Secondary IDs ( 24 )
Reported As
Symbol Synonym	CG3158 (Toledano-Katchalski et al., 2007, Keleman et al., 2009.8.5, Ni et al., 2008, Ni et al., 2011.7.19) fs(1)hls (Berg, 1994, ) fs(3)hls   hes (Stefanov et al., 2010) hls (Pal-Bhadra et al., 2004, Stapleton et al., 2001, Gillespie, 1996.2.16, Matsubayashi and Yamamoto, 1998, Ponting, 1997, Gillespie and Berg, 1995, Gillespie and Berg, 1995, Berg, 1994, Kavi and Birchler, 2007, Grimaud et al., 2006, Haynes et al., 2006, Kavi et al., 2006, Kavi and Birchler, 2008, Simmons et al., 2007, Josse et al., 2007, Kavi and Birchler, 2009, Peng and Karpen, 2007, Chambeyron et al., 2008, Cross and Simmons, 2008) homeless (Birchler et al., 2004) spin-E (Rouget et al., 2010) Spn-E (Klenov et al., 2004, Vagin et al., 2006, Vagin et al., 2006, Dorner et al., 2006, Georlette et al., 2007, Peng and Karpen, 2007, Nishida et al., 2009) spnE (Deng and Lin, 2001, Johnstone and Lasko, 2001, Huynh and St. Johnston, 2000, Morris et al., 2000, Ghabrial et al., 1998, Vagin et al., 2006, Snee and Macdonald, 2004, Wei and Rong, 2007, Meier et al., 2012, Anand and Kai, 2012, Chen et al., 2007, Navarro et al., 2009, Chambeyron et al., 2008, Robine et al., 2009, Hodgetts et al., 2012) spn E (Lasko, 2000) spn-E (McCaffrey et al., 2006, Klattenhoff et al., 2007, Klenov et al., 2007, Kogan et al., 2003, Lim and Kai, 2007, Shpiz et al., 2007, Shpiz et al., 2011, Pek and Kai, 2011, Kalmykova et al., 2005, Roy et al., 2007, Usakin et al., 2007, Kalmykova et al., 2008, Shpiz et al., 2008, Aravin et al., 2004, Xing et al., 2007, Tchurikov and Kretova, 2011, Castillo et al., 2011, Pane et al., 2007, Kawamura et al., 2008, Lim and Kai, 2007, Kalmykova et al., 2004, Blumenstiel et al., 2008, Malone et al., 2009, Shpiz et al., 2009, Lim et al., 2009, Robine et al., 2009, Venken et al., 2009, Heger and Ponting, 2007, Patil and Kai, 2010, Moshkovich and Lei, 2010, Nagao et al., 2010, Pek and Kai, 2011, Sharma et al., 2011, Roy et al., 2011, Ni et al., 2011, Seong et al., 2011, Saito et al., 2010, Handler et al., 2011, Stefanov et al., 2010, Rouget et al., 2010, Chotkowski et al., 2008, Klenov et al., 2011, Japanese National Institute of Genetics, 2012.5.21, van Bergeijk et al., 2012, Olivieri et al., 2012) SPN-E (Kolaczkowski et al., 2011, Janic et al., 2010) spn-E/hls (Cooperstock and Lipshitz, 1997)
Name Synonym	female sterile(3)homeless   homeless (Extavour and Akam, 2003, Wylie, 1999, de Cuevas et al., 1997, Williamson and Lehmann, 1996, Grunert and St. Johnston, 1996, Pal-Bhadra et al., 2004, Kavi et al., 2006, Ronsseray et al., 2008, Simmons et al., 2007, Josse et al., 2007, Chambeyron et al., 2008, Cross and Simmons, 2008, Specchia et al., 2010) Homeless (Anantharaman et al., 2002, Bernstein et al., 2001, Korbut et al., 2008, Szakmary et al., 2009) HOMELESS (Ronsseray et al., 2007) homeless/spindle-E (Zaratiegui, 2007) spindle-E (Lavorgna et al., 2004, Cook et al., 2004, Glaser et al., 2007, Lei and Corces, 2006, Klenov et al., 2007, Kogan et al., 2003, Wu and Silverman, 2007, Shpiz et al., 2007, Kalmykova et al., 2005, Roy et al., 2007, Aravin et al., 2004, Blumenstiel et al., 2008, Malone et al., 2009, Shpiz et al., 2009, Cross and Simmons, 2008, Patil and Kai, 2010, Olivieri et al., 2010, Seong et al., 2011, Handler et al., 2011) Spindle E (Klenov et al., 2003, Bhadra et al., 2007) spindle E (Xing et al., 2007, Specchia et al., 2010, Musters et al., 2006) Spindle-E (Carmi, 2006, Lei et al., 2008, Kawamura et al., 2008, Szakmary et al., 2009, Janic et al., 2010) SpindleE (Chambeyron et al., 2008) spindleE (Hodgetts et al., 2012) Spindle-E (homeless) (Berg, 2005)
Secondary FlyBase IDs
	FBgn0004883
References ( 177 )
Generate a list of	All references relating to this gene ( 177 )
List References by type	Research paper  ( 95 ) Supplementary material  ( 4 ) Review  ( 35 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 22 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Conference report  ( 1 ) Stock list  ( 2 ) Curated genome annotation  ( 3 ) Book  ( 1 )
Recent research papers ( 19 )
	Anand and Kai, 2012, EMBO J. 31(4): 870--882 The tudor domain protein Kumo is required to assemble the nuage and to generate germline piRNAs in Drosophila. [FBrf0217541] Hodgetts et al., 2012, Genome 55(4): 312--326 An intact RNA interference pathway is required for expression of the mutant wing phenotype of vg(21-3), a P-element-induced allele of the vestigial gene in Drosophila. [FBrf0217920] Lee and Langley, 2012, Genetics 192(4): 1411--1432 Long-term and short-term evolutionary impacts of transposable elements on Drosophila. [FBrf0220133] Meier et al., 2012, PLoS Genet. 8(5): e1002676 LINT, a Novel dL(3)mbt-Containing Complex, Represses Malignant Brain Tumour Signature Genes. [FBrf0218194] Olivieri et al., 2012, Mol. Cell 47(6): 954--969 The Cochaperone Shutdown Defines a Group of Biogenesis Factors Essential for All piRNA Populations in Drosophila. [FBrf0219572] van Bergeijk et al., 2012, PLoS ONE 7(5): e36539 Genome-Wide Expression Analysis Identifies a Modulator of Ionizing Radiation-Induced p53-Independent Apoptosis in Drosophila melanogaster. [FBrf0218467] Castillo et al., 2011, BMC Evol. Biol. 11: 258 Molecular evolution under increasing transposable element burden in Drosophila: A speed limit on the evolutionary arms race. [FBrf0216257] Handler et al., 2011, EMBO J. 30(19): 3977--3993 A systematic analysis of Drosophila TUDOR domain-containing proteins identifies Vreteno and the Tdrd12 family as essential primary piRNA pathway factors. [FBrf0216344] Kibanov et al., 2011, Mol. Biol. Cell 22(18): 3410--3419 A novel organelle, the piNG-body, in the nuage of Drosophila male germ cells is associated with piRNA-mediated gene silencing. [FBrf0215572] Klenov et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(46): 18760--18765 Separation of stem cell maintenance and transposon silencing functions of Piwi protein. [FBrf0216776] Kolaczkowski et al., 2011, Mol. Biol. Evol. 28(2): 1033--1042 Recurrent adaptation in RNA interference genes across the Drosophila phylogeny. [FBrf0212798] Ni et al., 2011, Nat. Methods 8(5): 405--407 A genome-scale shRNA resource for transgenic RNAi in Drosophila. [FBrf0213581] Pek and Kai, 2011, Proc. Natl. Acad. Sci. U.S.A. 108(29): 12007--12012 DEAD-box RNA helicase Belle/DDX3 and the RNA interference pathway promote mitotic chromosome segregation. [FBrf0214405] Pek and Kai, 2011, Curr. Biol. 21(1): 39--44 A role for vasa in regulating mitotic chromosome condensation in Drosophila. [FBrf0212718] Roy et al., 2011, Mol. Genet. Genomics 285(2): 113--123 Lack of the Drosophila BEAF insulator proteins alters regulation of genes in the Antennapedia complex. [FBrf0212912] Seong et al., 2011, Cell 145(7): 1049--1061 Inheritance of Stress-Induced, ATF-2-Dependent Epigenetic Change. [FBrf0214019] Sharma et al., 2011, Chemosphere 82(3): 370--376 Transcriptome analysis provides insights for understanding the adverse effects of endosulfan in Drosophila melanogaster. [FBrf0212610] Shpiz et al., 2011, Nucleic Acids Res. 39(20): 8703--8711 Mechanism of the piRNA-mediated silencing of Drosophila telomeric retrotransposons. [FBrf0216525] Tchurikov and Kretova, 2011, PLoS ONE 6(7): e21882 Both piRNA and siRNA Pathways Are Silencing Transcripts of the Suffix Element in the Drosophila melanogaster Germline and Somatic Cells. [FBrf0214474] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...