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Gene Dmel\spn-E

General Information
Symbol	Dmel\spn-E	Species	D. melanogaster
Name	spindle E	Annotation symbol	CG3158
Feature type	protein_coding_gene	FlyBase ID	FBgn0003483
Gene Model Status	Current	Stock availability	13 publicly available
Also Known As	hls, spnE
Genomic Location
Chromosome (arm)	3R	Recombination map	3-62
Cytogenetic map	89A5-89A5	Sequence location	3R:11,663,159..11,670,138 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene spindle E is referred to in FlyBase by the symbol Dmel\spn-E (CG3158, FBgn0003483). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: helicase activity; ATP-dependent RNA helicase activity. There is experimental evidence for 15 unique biological process terms, many of which group under: cellular component organization or biogenesis; cellular process involved in reproduction; multicellular organismal reproductive process; biological regulation; anatomical structure development; localization; anterior/posterior axis specification; dorsal/ventral axis specification; regulation of cellular macromolecule biosynthetic process; positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; cell cycle; polarity specification of dorsal/ventral axis; regulation of multicellular organismal development. 34 alleles are reported. The phenotypes of these alleles are annotated with: germline cyst; P granule; oocyte; karyosome; spermatocyte; egg chamber; microtubule; dorsal appendage; synaptonemal complex. It has one annotated transcript and one annotated polypeptide. Protein features are: DEAD-like helicase; DNA/RNA helicase, DEAD/DEAH box type, N-terminal; Helicase, C-terminal; Helicase-associated domain; Maternal tudor protein; Tudor domain; Tudor subgroup; Zinc finger, C2H2; Zinc finger, C2H2-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1636886_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of spn-E. Gene sequence location is 3R:11663159..11670138.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	spn-E: spindle E Maternal-effect lethal. Similar to spn-A.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10	References Kibanov et al., 2011, Mol. Biol. Cell 22(18): 3410--3419 Saito et al., 2010, Genes Dev. 24(22): 2493--2498
FB2012_01	References Handler et al., 2011, EMBO J. 30(19): 3977--3993 Rouget et al., 2010, Nature 467(7319): 1128--1132 Shpiz et al., 2011, Nucleic Acids Res. 39(20): 8703--8711 Stefanov et al., 2010, Mol. Genet. Genomics 284(5): 373--381 Controlled Vocabulary Terms positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Sequence features BKN21576 HFA17064
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 89A5-89A5   Limits computationally determined from genome sequence between P{PZ}l(3)0653606536 and P{PZ}blp01618
Experimentally Determined Cytological Location
Cytogenetic map Notes References 89B1-89B2   (determined by in situ hybridisation)   (Spradling et al., 1999) 89A5-89A6   (determined by in situ hybridisation)   (Gillespie and Berg, 1995) 89A5-89A6   (determined by in situ hybridisation)   (Berg, 1994) 89B1-89B2     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	3-62 (Tearle and Nusslein-Volhard, 1987, Gonzalez-Reyes et al., 1997)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\spn-E for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) spn-E-RA FBtr0083183  NM_057393   4661   1434
Additional Transcript Data & Comments
Reported size (kB)	4.6 (northern blot) (Gillespie and Berg, 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein spn-E-PA   FBpp0082637   164.5   1434   7.22     NP_476741   AAF55235
Additional Polypeptide Data & Comments
Reported size (kDa)	1441 (aa); 155 (kD predicted) (Gillespie and Berg, 1995)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Tudor subgroup (IPR018351) Zinc finger, C2H2-like (IPR015880) Helicase, C-terminal (IPR001650) Tudor domain (IPR002999) Zinc finger, C2H2 (IPR007087) Helicase-associated domain (IPR007502) Maternal tudor protein (IPR008191) DEAD-like helicase (IPR014001) DNA/RNA helicase, DEAD/DEAH box type, N-terminal (IPR011545)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA142297   AE014297 AAF55235   AC010122   AQ025574   BP559549   BP559550   BT029921   BT100306   CO267601   CZ466715   CZ471194   CZ481218   CZ481219   CZ481860   EC083913   EC084674   EL871700   EL871701   EL871823   EL871824   EL871953   EL871954   GH943009   GH943732   GH943896   GH944462   GH944854   GH947757   GH949818   GH949987   GH950044   GH950511   GH950896   GH954075   GH954899   GH955531   GH958151   GH960212   GH961164   GH964422   GH969215   GH970178   GH972678   GH973298   GH978641   S79915 AAB35476
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VF26
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage | early   organism | ubiquitous   (Gillespie and Berg, 1995) adult stage & oogenesis   germarium   (Gillespie and Berg, 1995) female germline stem cell   (Gillespie and Berg, 1995) nurse cell   (Gillespie and Berg, 1995) oogenesis & adult stage | female   ovary   (Gillespie and Berg, 1995)
Additional Descriptive Data
	spn-E transcripts are expressed predominantly in ovaries but small amounts are detected in total male and female carcass RNA. In the germarium, expression is limited to the germline cells. Expression decreases through oogenesis stages S4/S5, increases during S8 and peaks in nurse cells at stage S10. No transcript is detected in the oocyte until stage S11 when it is deposited from the nurse cells. spn-E transcripts are uniformly distributed in early embryos. (Gillespie and Berg, 1995)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0003483    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0003483 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0003483 expression level Developmental Stage   Expression Level embryo 00-02hr    2962 embryo 02-04hr    1079 embryo 04-06hr    318 embryo 06-08hr    143 embryo 08-10hr    188 embryo 10-12hr    99 embryo 12-14hr    133 embryo 14-16hr    160 embryo 16-18hr    129 embryo 18-20hr    75 embryo 20-22hr    177 embryo 22-24hr    180 larva L1    52 larva L2    54 larva L3 12hr old    47 larva L3 puffstage 1-2    64 larva L3 puffstage 3-6    96 larva L3 puffstage 7-9    121 white prepupae new    128 white prepupae 12hr    195 white prepupae 24hr    167 pupae 2d postWPP    123 pupae 3d postWPP    95 pupae 4d postWPP    150 adult male 01day    117 adult male 05day    179 adult male 30day    179 adult female 01day    966 adult female 05day    1882 adult female 30day    1783 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2962) embryo 02-04hr  (1079) embryo 04-06hr  (318) embryo 06-08hr  (143) embryo 08-10hr  (188) embryo 10-12hr    99 embryo 12-14hr  (133) embryo 14-16hr  (160) embryo 16-18hr  (129) embryo 18-20hr    75 embryo 20-22hr  (177) embryo 22-24hr  (180) larva L1    52 larva L2    54 larva L3 12hr old    47 larva L3 puffstage 1-2    64 larva L3 puffstage 3-6    96 larva L3 puffstage 7-9  (121) white prepupae new  (128) white prepupae 12hr  (195) white prepupae 24hr  (167) pupae 2d postWPP  (123) pupae 3d postWPP    95 pupae 4d postWPP  (150) adult male 01day  (117) adult male 05day  (179) adult male 30day  (179) adult female 01day  (966) adult female 05day  (1882) adult female 30day  (1783) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2962) embryo 02-04hr    1079 embryo 04-06hr    318 embryo 06-08hr    143 embryo 08-10hr    188 embryo 10-12hr    99 embryo 12-14hr    133 embryo 14-16hr    160 embryo 16-18hr    129 embryo 18-20hr    75 embryo 20-22hr    177 embryo 22-24hr    180 larva L1    52 larva L2    54 larva L3 12hr old    47 larva L3 puffstage 1-2    64 larva L3 puffstage 3-6    96 larva L3 puffstage 7-9    121 white prepupae new    128 white prepupae 12hr    195 white prepupae 24hr    167 pupae 2d postWPP    123 pupae 3d postWPP    95 pupae 4d postWPP    150 adult male 01day    117 adult male 05day    179 adult male 30day    179 adult female 01day    966 adult female 05day  (1882) adult female 30day  (1783) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2962 embryo 02-04hr    1079 embryo 04-06hr    318 embryo 06-08hr    143 embryo 08-10hr    188 embryo 10-12hr    99 embryo 12-14hr    133 embryo 14-16hr    160 embryo 16-18hr    129 embryo 18-20hr    75 embryo 20-22hr    177 embryo 22-24hr    180 larva L1    52 larva L2    54 larva L3 12hr old    47 larva L3 puffstage 1-2    64 larva L3 puffstage 3-6    96 larva L3 puffstage 7-9    121 white prepupae new    128 white prepupae 12hr    195 white prepupae 24hr    167 pupae 2d postWPP    123 pupae 3d postWPP    95 pupae 4d postWPP    150 adult male 01day    117 adult male 05day    179 adult male 30day    179 adult female 01day    966 adult female 05day    1882 adult female 30day    1783 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2962 embryo 02-04hr    1079 embryo 04-06hr    318 embryo 06-08hr    143 embryo 08-10hr    188 embryo 10-12hr    99 embryo 12-14hr    133 embryo 14-16hr    160 embryo 16-18hr    129 embryo 18-20hr    75 embryo 20-22hr    177 embryo 22-24hr    180 larva L1    52 larva L2    54 larva L3 12hr old    47 larva L3 puffstage 1-2    64 larva L3 puffstage 3-6    96 larva L3 puffstage 7-9    121 white prepupae new    128 white prepupae 12hr    195 white prepupae 24hr    167 pupae 2d postWPP    123 pupae 3d postWPP    95 pupae 4d postWPP    150 adult male 01day    117 adult male 05day    179 adult male 30day    179 adult female 01day    966 adult female 05day    1882 adult female 30day    1783 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0003483 expression level Developmental Stage   Expression Level embryo 00-02hr    2962 embryo 02-04hr    1079 embryo 04-06hr    318 embryo 06-08hr    143 embryo 08-10hr    188 embryo 10-12hr    99 embryo 12-14hr    133 embryo 14-16hr    160 embryo 16-18hr    129 embryo 18-20hr    75 embryo 20-22hr    177 embryo 22-24hr    180 larva L1    52 larva L2    54 larva L3 12hr old    47 larva L3 puffstage 1-2    64 larva L3 puffstage 3-6    96 larva L3 puffstage 7-9    121 white prepupae new    128 white prepupae 12hr    195 white prepupae 24hr    167 pupae 2d postWPP    123 pupae 3d postWPP    95 pupae 4d postWPP    150 adult male 01day    117 adult male 05day    179 adult male 30day    179 adult female 01day    966 adult female 05day    1882 adult female 30day    1783 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2962) embryo 02-04hr  (1079) embryo 04-06hr  (318) embryo 06-08hr  143 embryo 08-10hr  (188) embryo 10-12hr    99 embryo 12-14hr  133 embryo 14-16hr  (160) embryo 16-18hr  129 embryo 18-20hr    75 embryo 20-22hr  (177) embryo 22-24hr  (180) larva L1    52 larva L2    54 larva L3 12hr old    47 larva L3 puffstage 1-2    64 larva L3 puffstage 3-6    96 larva L3 puffstage 7-9  121 white prepupae new  128 white prepupae 12hr  (195) white prepupae 24hr  (167) pupae 2d postWPP  123 pupae 3d postWPP    95 pupae 4d postWPP  (150) adult male 01day  117 adult male 05day  (179) adult male 30day  (179) adult female 01day  (966) adult female 05day  (1882) adult female 30day  (1783) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2962) embryo 02-04hr    1079 embryo 04-06hr    318 embryo 06-08hr    143 embryo 08-10hr    188 embryo 10-12hr    99 embryo 12-14hr    133 embryo 14-16hr    160 embryo 16-18hr    129 embryo 18-20hr    75 embryo 20-22hr    177 embryo 22-24hr    180 larva L1    52 larva L2    54 larva L3 12hr old    47 larva L3 puffstage 1-2    64 larva L3 puffstage 3-6    96 larva L3 puffstage 7-9    121 white prepupae new    128 white prepupae 12hr    195 white prepupae 24hr    167 pupae 2d postWPP    123 pupae 3d postWPP    95 pupae 4d postWPP    150 adult male 01day    117 adult male 05day    179 adult male 30day    179 adult female 01day    966 adult female 05day  1882 adult female 30day  1783 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2962 embryo 02-04hr    1079 embryo 04-06hr    318 embryo 06-08hr    143 embryo 08-10hr    188 embryo 10-12hr    99 embryo 12-14hr    133 embryo 14-16hr    160 embryo 16-18hr    129 embryo 18-20hr    75 embryo 20-22hr    177 embryo 22-24hr    180 larva L1    52 larva L2    54 larva L3 12hr old    47 larva L3 puffstage 1-2    64 larva L3 puffstage 3-6    96 larva L3 puffstage 7-9    121 white prepupae new    128 white prepupae 12hr    195 white prepupae 24hr    167 pupae 2d postWPP    123 pupae 3d postWPP    95 pupae 4d postWPP    150 adult male 01day    117 adult male 05day    179 adult male 30day    179 adult female 01day    966 adult female 05day    1882 adult female 30day    1783 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2962 embryo 02-04hr    1079 embryo 04-06hr    318 embryo 06-08hr    143 embryo 08-10hr    188 embryo 10-12hr    99 embryo 12-14hr    133 embryo 14-16hr    160 embryo 16-18hr    129 embryo 18-20hr    75 embryo 20-22hr    177 embryo 22-24hr    180 larva L1    52 larva L2    54 larva L3 12hr old    47 larva L3 puffstage 1-2    64 larva L3 puffstage 3-6    96 larva L3 puffstage 7-9    121 white prepupae new    128 white prepupae 12hr    195 white prepupae 24hr    167 pupae 2d postWPP    123 pupae 3d postWPP    95 pupae 4d postWPP    150 adult male 01day    117 adult male 05day    179 adult male 30day    179 adult female 01day    966 adult female 05day    1882 adult female 30day    1783 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0003483    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0003483 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0003483 expression level Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland no informative data Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland no informative data Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland no informative data Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland no informative data Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland no informative data Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland no informative data Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland no informative data Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland no informative data Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0003483 expression level Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland no informative data Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland no informative data Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland no informative data Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland no informative data Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland no informative data Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland no informative data Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    49.15 Larval Midgut    8.2 Larval Hindgut    8.9 Larval Malpighian Tubules    5.3 Larval Fat Body    53.5 Larval Salivary Gland no informative data Larval Trachea    12.15 Larval Carcass    12.7 Adult Head    10.1 Adult Eye    33.575 Adult Brain    4.4 Adult Thoracic-Abdominal Ganglion    5.7 Adult Crop    5.9 Adult Midgut    8.1 Adult Hindgut    5.3 Adult Malpighian Tubules    5.4 Adult Fat Body    11.2 Adult Salivary Gland no informative data Adult Heart    6.525 Adult VirginFemale Spermatheca    6.5 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    466.7 Adult Testis    66.6 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland no informative data Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    10.1   NA  Eye    33.575   NA  Brain    4.4   49.15  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    5.7   NA  Crop    5.9   8.2  Midgut    8.1   8.9  Hindgut    5.3   5.3  Malpighian Tubules    5.4   53.5  Fat Body    11.2   no informative data  Salivary Gland    no informative data   NA  Heart    6.525   12.15  Trachea    NA   NA  VirginFemale Spermatheca    6.5   NA  InseminatedFemale Spermatheca    8.6   NA  Ovary    466.7   NA  Testis    66.6   NA  Male Accessory Gland    no informative data   12.7  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_13
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003483 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3158-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | recessive spn-Ehls-Δ125 lethal | polyphasic | recessive spn-Ehls-Δ139 spn-Ehls-Δ157 lethal | recessive spn-Ehls-Δ125 spn-Ehls-Δ157 viable, with Scer\GAL4pnr-MD237 spn-EGD10299 Sterility Allele female sterile spn-E167 spn-E167R33 spn-Ehls-03987 female sterile, with Scer\GAL4nos.PG, Scer\GAL4nos.UTR.T:Hsim\VP16, Scer\GAL4otu.T:Hsim\VP16 spn-EdsRNA.shRNA.Scer\UAS.P\T female sterile | recessive spn-Ehls-03987 female sterile germline-dependent, with Scer\GAL4nos.PG, Scer\GAL4nos.UTR.T:Hsim\VP16, Scer\GAL4otu.T:Hsim\VP16 spn-EdsRNA.shRNA.Scer\UAS.P\T fertile (with spn-E1) spn-Ehls-Δ125 fertile (with spn-Ehls-Δ125) spn-E1 male fertile spn-E167 spn-E167R33 male sterile (with spn-E1) spn-Ehls-Δ125 male sterile (with spn-Ehls-Δ125) spn-E1 male sterile | partially spn-E1 sterile (with spn-Ehls-03987) spn-Ehls-Δ139 spn-Ehls-Δ157 sterile (with spn-Ehls-Δ139) spn-Ehls-03987 sterile (with spn-Ehls-Δ157) spn-Ehls-03987 Other Phenotypes Allele meiotic cell cycle defective spn-E1 spn-E2 spn-Ehls-03987 meiotic cell cycle defective (with spn-E1) spn-E2 spn-Ehls-03987 spn-Ehls-Δ125 meiotic cell cycle defective (with spn-E2) spn-E1 meiotic cell cycle defective (with spn-Ehls-03987) spn-E1 meiotic cell cycle defective (with spn-Ehls-Δ125) spn-E1 suppressor of variegation spn-E1 spn-EDE8 spn-Ehls-03987 spn-Ehls-E1 spn-Ehls-Δ125 suppressor of variegation, with P{T1(-FRT)}T190-177 spn-Ehls-Δ125 suppressor of variegation, with P{T1}T190-177 spn-Ehls-Δ125 Phenotype manifest in Allele dorsal appendage spn-E1 spn-E167 spn-E167R33 spn-Ehls-03987 egg | dorsal spn-E1 egg chamber spn-E167R33 spn-Ehls-03987 egg chamber | germline clone spn-Eunspecified germline cyst spn-E1 karyosome spn-E1 spn-Eunspecified meiosis & nuclear chromosome spn-E1 spn-E2 spn-Ehls-03987 meiosis & nuclear chromosome (with spn-E1) spn-E2 spn-Ehls-Δ125 spn-Ehls-03987 meiosis & nuclear chromosome (with spn-E2) spn-E1 meiosis & nuclear chromosome (with spn-Ehls-03987) spn-E1 meiosis & nuclear chromosome (with spn-Ehls-Δ125) spn-E1 meiosis & nuclear chromosome | male spn-E1 microtubule spn-Ehls-03987 oocyte spn-E1 spn-Ehls-03987 oocyte (with spn-E4E2-14) spn-Ehls-Δ157 oocyte (with spn-Ehls-Δ157) spn-E4E2-14 P granule (with spn-E1) spn-Ehls-03987 P granule (with spn-Ehls-03987) spn-E1 spermatocyte spn-E1 synaptonemal complex spn-E1
Classical Alleles ( 25 )
For All Classical Alleles Show
Allele of spn-E Class Mutagen Stocks Known lesion spn-E1 hypomorphic allele - genetic evidence ethyl methanesulfonate 2 -- spn-Ec00786 piggyBac transposase 2 -- spn-Ec07043 piggyBac transposase 1 -- spn-Ee03441 piggyBac transposase 1 -- spn-Ehls-03987 hypomorphic allele - genetic evidence P-element activity 1 Yes spn-E167R33 amorphic allele - genetic evidence Delta2-3 0 -- spn-E100.37 0 -- spn-E125 0 -- spn-E167 hypomorphic allele - genetic evidence Delta2-3 0 -- spn-E167R18 Delta2-3 0 -- spn-E2 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- spn-E2A9-14 ethyl methanesulfonate 0 -- spn-E4E2-14 ethyl methanesulfonate 0 -- spn-E7G2-5 ethyl methanesulfonate 0 -- spn-E8D4-11 ethyl methanesulfonate 0 -- spn-E9A2-17 ethyl methanesulfonate 0 -- spn-E9A9-18 ethyl methanesulfonate 0 -- spn-ED58 0 -- spn-EDE8 0 -- spn-Ee04103 piggyBac transposase 0 -- spn-Ehls-E1 0 -- spn-Ehls-Δ125 loss of function allele Delta2-3 0 Yes spn-Ehls-Δ139 Delta2-3 0 Yes spn-Ehls-Δ157 loss of function allele Delta2-3 0 Yes spn-Eunspecified 0 --
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of spn-E Class Mutagen Stocks Known lesion spn-EGD10299 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes spn-EGL00206 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spn-EHMS00117 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spn-EJF01405 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spn-EKK101525 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spn-EdsRNA.cDa in vitro construct - RNAi 0 Yes spn-EdsRNA.shRNA.Scer\UAS.P\T in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes spn-ET:Uuuu\T7 in vitro construct - coding region fusion 0 Yes spn-EtCH322-93D04 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)160R34 (Matsubayashi and Yamamoto, 1998) Df(3R)160R104 (Matsubayashi and Yamamoto, 1998) Df(3R)167R11 (Matsubayashi and Yamamoto, 1998) Df(3R)167R17 (Matsubayashi and Yamamoto, 1998) Df(3R)167R37 (Matsubayashi and Yamamoto, 1998) Df(3R)c(3)G-2 (Matsubayashi and Yamamoto, 1998) Df(3R)Exel6175 (Ryder, 2004.4.26) Df(3R)Exel8162 (Ryder, 2004.4.26) Df(3R)Po2 (Matsubayashi and Yamamoto, 1998) Df(3R)R24 (Matsubayashi and Yamamoto, 1998) Df(3R)R44 (Matsubayashi and Yamamoto, 1998) Df(3R)sbd105 (Gonzalez-Reyes et al., 1997, Gillespie and Berg, 1995)
Not disrupted in	Df(3R)160R5 (Matsubayashi and Yamamoto, 1998) Df(3R)160R8 (Matsubayashi and Yamamoto, 1998) Df(3R)160R32 (Matsubayashi and Yamamoto, 1998)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10299} NA P{KK101525} NA P{TRiP.GL00206} NA P{TRiP.HMS00117} NA P{TRiP.JF01405} NA P{UASp-spn-E.shRNA} NA characterization construct PBac{CH322-93D04} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}spn-Ec00786 NA PBac{PB}spn-Ec07043 NA PBac{RB}spn-Ee03441 NA PBac{RB}spn-Ee04103 NA insertion of enhancer trap P{lwB}spn-E167 No P{lwB}spn-E167R18 No P{lwB}spn-E167R33 No P{PZ}spn-Ehls-03987 No
Gene Ontology: Function, Process & Cellular Component ( 29 unique terms )
Terms Based on Experimental Evidence ( 18 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
chromatin silencing inferred from mutant phenotype (Pal-Bhadra et al., 2004) dorsal appendage formation inferred from mutant phenotype (Klattenhoff et al., 2007) germarium-derived oocyte fate determination inferred from genetic interaction with mus301 (Gonzalez-Reyes et al., 1997) intracellular mRNA localization inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) karyosome formation inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) mitotic chromosome condensation inferred from mutant phenotype (Pek and Kai, 2011) oocyte localization involved in germarium-derived egg chamber formation inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) oocyte maturation inferred from mutant phenotype (Kennerdell et al., 2002) oogenesis inferred from genetic interaction with fs(1)K10 (Gonzalez-Reyes et al., 1997) polarity specification of anterior/posterior axis inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) polarity specification of dorsal/ventral axis inferred from mutant phenotype (Gonzalez-Reyes et al., 1997) pole plasm oskar mRNA localization inferred from mutant phenotype (Martin et al., 2003) positive regulation of nuclear-transcribed mRNA poly(A) tail shortening inferred from mutant phenotype (Rouget et al., 2010) regulation of pole plasm oskar mRNA localization inferred from mutant phenotype (Cook et al., 2004) targeting of mRNA for destruction involved in RNA interference inferred from mutant phenotype (Kennerdell et al., 2002)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Grimaud et al., 2006) nucleus inferred from direct assay (Grimaud et al., 2006) P granule inferred from direct assay (Patil and Kai, 2010)
Terms Based on Predictions or Assertions ( 14 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR001650, InterPro:IPR011545 (FlyBase Curators et al., 2004-) ATP-dependent RNA helicase activity inferred from sequence or structural similarity with InterPro:IPR001410 (FlyBase, 1992-) helicase activity inferred from sequence or structural similarity (Lasko, 2000) nucleic acid binding inferred from electronic annotation with InterPro:IPR001650, InterPro:IPR011545 (FlyBase Curators et al., 2004-) RNA-dependent ATPase activity traceable author statement (Wylie, 1999) RNA helicase activity traceable author statement (van Eeden and St. Johnston, 1999) zinc ion binding inferred from electronic annotation with InterPro:IPR007087, InterPro:IPR015880 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
intracellular mRNA localization traceable author statement (Cooperstock and Lipshitz, 1997) oocyte differentiation non-traceable author statement (Deng and Lin, 2001) oogenesis traceable author statement (Wylie, 1999, van Eeden and St. Johnston, 1999) pole plasm assembly non-traceable author statement (Williamson and Lehmann, 1996) RNA interference traceable author statement (Lavorgna et al., 2004, Murchison and Hannon, 2004) RNA localization traceable author statement (Wylie, 1999)
Cellular Component
CV term Evidence References
cytoplasm non-traceable author statement (Morris and Lehmann, 1999)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions spn-E allele Gene References spn-E1 spn-D (Gonzalez-Reyes et al., 1997) Ste (Stapleton et al., 2001) spn-Ehls-Δ125 Ste (Stapleton et al., 2001) unspecified spn-B (Gonzalez-Reyes et al., 1997)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66977 DroID - A comprehensive database of gene and protein interactions. • FBgn0003483 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41919
Orthologs
Genome-wide drosophilid orthologs	Dana\GF18547 Dere\GG16958 Dgri\GH23451 Dmoj\GI22949 Dper\GL22075 Dpse\GA16329 Dsec\GM24265 Dsim\GD19053 Dvir\GJ23765 Dwil\GK11214 Dyak\GE24344
Curated drosophilid orthologs	Dpse\GA16329
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL013235-PA Aedes aegypti (mosquito) AGAP002829-PA Anopheles gambiae (mosquito) XP_001122827.1 Apis mellifera (honey bee) 65715 Branchiostoma floridae (lancelet) BGIBMGA004949-PA Bombyx mori (silk worm) ENSCAFP00000027055 Canis familiaris (dog) CPIJ017541 Culex pipens (mosquito) ENSCPOP00000002506 Cavia porcellus (guinea pig) ENSCSAVP00000003618 Ciona savignyi (sedentary tunicate) 316598 Daphnia pulex (daphnia) ENSECAP00000021540 Equus caballus (horse) ENSGACP00000011040 Gymnopilus aculeatus (mushroom) ENSGALP00000018840 Gallus gallus (chicken) ENSP00000387303 Homo sapiens (human) 110746 Lottia gigantea (owl limpet) ENSMMUP00000008216 Macaca mulatta (rhesus monkey) ENSMUSP00000078022 Mus musculus (house mouse) XP_001600067.1 Nasonia vitripennis (wasp) ENSOANP00000001235 Ornithorynchus anatinus (platypus) ENSORLP00000019840 Oryzias latipes (japanese killifish) PHUM005771-PA Pediculus humanus (human body louse) ENSPPYP00000007024 Pongo pygmaeus (orangutan) ENSPTRP00000044404 Pan troglodytes (chimpanzee) XP_971741.1 Tribolium castaneum (red flour beetle) ENSTNIP00000020491 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000024088 Takifugu rubripes (pufferfish Takifugu) 3836.m02132 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000049887 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13901 PB11696 NV11276 LH14588 LH23051 FBgn0241465 FBgn0213225 FBgn0210862 FBgn0190560 FBgn0179129 JGI_V11_316598 FBgn0076345 FBgn0159667 FBgn0145676 FBgn0130908 FBgn0109186 FBgn0095565 CPIJ017541 BGIBMGA004949 ACYPI007191 GB13563 AGAP002829 AAEL013235 PHUM492140 GLEAN_14516
Stocks & Reagents
Stocks Listed in FlyBase ( 13 )
Bloomington	31620 y1 v1; P{TRiP.JF01405}attP2 35303 y1 sc* v1; P{TRiP.GL00206}attP2 34808 y1 sc* v1; P{TRiP.HMS00117}attP2 10274 w1118; PBac{PB}spn-Ec00786/TM6B, Tb1 3327 ru1 st1 spn-E1 e1 ca1/TM3, Sb1 Ser1 24853 mwh1 ry506 P{PZ}spn-Ehls-03987 es/TM3, ryRK Sb1
Harvard	- PBac{PB}spn-Ec00786 - PBac{PB}spn-Ec07043 - PBac{RB}spn-Ee03441
VDRC	v103913 P{KK101525}VIE-260B v21376 w1118; P{GD10299}v21376 v21374 w1118; P{GD10299}v21374/TM3
	More stocks available...
Genomic Clones ( 1 )
	BACR06B08 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 33 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones	FI13017 IP03663
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	31122 38618 CK01654 EK151039 FGM321E10 IP03763 IP03863 IP03963 RP004042001 RP004094296 RP004106336 RP004120062 RP004164037 RP004171435 RP004177803 RP004178554 RP004180611 RP004205358 RP004237204 RP004240366 RP004246114 RP004248147 RP004252132 RP004257601 RP004384232 RP004394487 RP004430887 RP004444415 RP004504120 RP004537884 RP004547222
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003483 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41919
Antibody Information
	polyclonal (Patil and Kai, 2010)
Other Information
Discoverer
Etymology
	Named "homeless" based on oocyte mislocalisation phenotype and RNA localisation phenotype. (Gillespie and Berg, 1995)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: spn-E CG3158 (Kennison, 1999.11)
Source for database merge of
Additional comments
Other Comments
	spn-E is required for the accumulation of repeat-associated small interfering RNAs (rasiRNAs). (Vagin et al., 2006) piwi, aub and spn-E are suppressors of repeat-induced silencing, as seen at heterochromatic tandem arrays of insertions of the P{lacW} transposon. (Pal-Bhadra et al., 2004) spn-E mutations have a dramatic effect on the distribution of Su(var)205 protein. Instead of the Su(var)205 protein being localised to pericentric heterochromatin and the fourth chromosome it is distributed across the whole of the polytene chromosomes. There is a reduction in the levels of the methyl-K9 form of His3. (Pal-Bhadra et al., 2004) Identification: in a germline clone screen for mutants that are defective in localisation of an Avic\GFP-stau marker in living oocytes. 6 alleles of spn-E have been identified in the screen. (Martin et al., 2003) spn-E mutants block RNAi activation which normally occurs during egg maturation. (Kennerdell et al., 2002) spn-E has a role in repressing the Ste locus and may have a role in repressing other repeated sequences. (Stapleton et al., 2001) Analysis of mutants of the 5 spindle genes (spn-A, spn-B, mus301, spn-D and spn-E) reveals that the group of genes is required for each of the symmetry-breaking steps that generate polarity during axis formation. spn-E is required for the localisation of fs(1)K10, bcd and osk mRNAs, spn-A. spn-B, mus301 and spn-D play an indirect role in this process. (Gonzalez-Reyes et al., 1997) spn-E gene product is necessary for the correct localisation of several RNAs that are involved in anteroposterior and dorsoventral axis formation during oogenesis. (Gillespie and Berg, 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057393 RefSeq (Proteins) • NP_476741 DDBJ / EMBL / GenBank • AAB35476 AAF55235 AC010122 AQ025574 CZ466715 CZ471194 CZ481218 CZ481219 CZ481860 S79915 Entrez Gene - A searchable database of RefSeq genes. • 41919 UniProtKB/TrEMBL • Q9VF26
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Tudor subgroup (IPR018351) Zinc finger, C2H2-like (IPR015880) Helicase, C-terminal (IPR001650) Tudor domain (IPR002999) Zinc finger, C2H2 (IPR007087) Helicase-associated domain (IPR007502) Maternal tudor protein (IPR008191) DEAD-like helicase (IPR014001) DNA/RNA helicase, DEAD/DEAH box type, N-terminal (IPR011545)
Linkouts
	BioGRID - A database of protein and genetic interactions • 66977 DroID - A comprehensive database of gene and protein interactions. • FBgn0003483 DRSC - Results from RNAi screens. • FBgn0003483 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003483 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3158-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003483 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41919 InParanoid A subset of ortholog calls from InParanoid. • AAEL013235-PA Aedes aegypti (mosquito) AGAP002829-PA Anopheles gambiae (mosquito) XP_001122827.1 Apis mellifera (honey bee) 65715 Branchiostoma floridae (lancelet) BGIBMGA004949-PA Bombyx mori (silk worm) ENSCAFP00000027055 Canis familiaris (dog) CPIJ017541 Culex pipens (mosquito) ENSCPOP00000002506 Cavia porcellus (guinea pig) ENSCSAVP00000003618 Ciona savignyi (sedentary tunicate) 316598 Daphnia pulex (daphnia) ENSECAP00000021540 Equus caballus (horse) ENSGACP00000011040 Gymnopilus aculeatus (mushroom) ENSGALP00000018840 Gallus gallus (chicken) ENSP00000387303 Homo sapiens (human) 110746 Lottia gigantea (owl limpet) ENSMMUP00000008216 Macaca mulatta (rhesus monkey) ENSMUSP00000078022 Mus musculus (house mouse) XP_001600067.1 Nasonia vitripennis (wasp) ENSOANP00000001235 Ornithorynchus anatinus (platypus) ENSORLP00000019840 Oryzias latipes (japanese killifish) PHUM005771-PA Pediculus humanus (human body louse) ENSPPYP00000007024 Pongo pygmaeus (orangutan) ENSPTRP00000044404 Pan troglodytes (chimpanzee) XP_971741.1 Tribolium castaneum (red flour beetle) ENSTNIP00000020491 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000024088 Takifugu rubripes (pufferfish Takifugu) 3836.m02132 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000049887 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/homels1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41919 modMine - Data generated by the modENCODE project. • FBgn0003483 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13901 PB11696 NV11276 LH14588 LH23051 FBgn0241465 FBgn0213225 FBgn0210862 FBgn0190560 FBgn0179129 JGI_V11_316598 FBgn0076345 FBgn0159667 FBgn0145676 FBgn0130908 FBgn0109186 FBgn0095565 CPIJ017541 BGIBMGA004949 ACYPI007191 GB13563 AGAP002829 AAEL013235 PHUM492140 GLEAN_14516
Synonyms & Secondary IDs ( 23 )
Reported As
Symbol Synonym	CG3158 (Toledano-Katchalski et al., 2007, Keleman et al., 2009.8.5, Ni et al., 2008, Ni et al., 2011.7.19) fs(1)hls (Berg, 1994, ) fs(3)hls   hes (Stefanov et al., 2010) hls (Pal-Bhadra et al., 2004, Stapleton et al., 2001, Gillespie, 1996.2.16, Matsubayashi and Yamamoto, 1998, Ponting, 1997, Gillespie and Berg, 1995, Gillespie and Berg, 1995, Berg, 1994, Kavi and Birchler, 2007, Grimaud et al., 2006, Haynes et al., 2006, Kavi et al., 2006, Kavi and Birchler, 2008, Simmons et al., 2007, Josse et al., 2007, Peng and Karpen, 2007, Chambeyron et al., 2008, Cross and Simmons, 2008) homeless (Birchler et al., 2004) spin-E (Rouget et al., 2010) Spn-E (Klenov et al., 2004, Vagin et al., 2006, Vagin et al., 2006, Dorner et al., 2006, Georlette et al., 2007, Peng and Karpen, 2007, Nishida et al., 2009) spnE (Deng and Lin, 2001, Johnstone and Lasko, 2001, Huynh and St. Johnston, 2000, Morris et al., 2000, Ghabrial et al., 1998, Vagin et al., 2006, Snee and Macdonald, 2004, Wei and Rong, 2007, Chen et al., 2007, Navarro et al., 2009, Chambeyron et al., 2008, Robine et al., 2009) spn E (Lasko, 2000) spn-E (McCaffrey et al., 2006, Klattenhoff et al., 2007, Klenov et al., 2007, Kogan et al., 2003, Lim and Kai, 2007, Shpiz et al., 2007, Pek and Kai, 2011, Kalmykova et al., 2005, Roy et al., 2007, Usakin et al., 2007, Kalmykova et al., 2008, Shpiz et al., 2008, Aravin et al., 2004, Xing et al., 2007, Pane et al., 2007, Kawamura et al., 2008, Lim and Kai, 2007, Kalmykova et al., 2004, Blumenstiel et al., 2008, Malone et al., 2009, Shpiz et al., 2009, Lim et al., 2009, Robine et al., 2009, Venken et al., 2009, Heger and Ponting, 2007, Patil and Kai, 2010, Moshkovich and Lei, 2010, Nagao et al., 2010, Pek and Kai, 2011, Sharma et al., 2011, Roy et al., 2011, Ni et al., 2011, Seong et al., 2011, Saito et al., 2010, Handler et al., 2011, Stefanov et al., 2010, Rouget et al., 2010) SPN-E (Janic et al., 2010) spn-E/hls (Cooperstock and Lipshitz, 1997)
Name Synonym	female sterile(3)homeless   homeless (Extavour and Akam, 2003, Wylie, 1999, de Cuevas et al., 1997, Williamson and Lehmann, 1996, Grunert and St. Johnston, 1996, Pal-Bhadra et al., 2004, Kavi et al., 2006, Ronsseray et al., 2008, Simmons et al., 2007, Josse et al., 2007, Chambeyron et al., 2008, Cross and Simmons, 2008) Homeless (Anantharaman et al., 2002, Bernstein et al., 2001, Korbut et al., 2008, Szakmary et al., 2009) HOMELESS (Ronsseray et al., 2007) homeless/spindle-E (Zaratiegui, 2007) spindle-E (Lavorgna et al., 2004, Cook et al., 2004, Glaser et al., 2007, Lei and Corces, 2006, Klenov et al., 2007, Kogan et al., 2003, Wu and Silverman, 2007, Shpiz et al., 2007, Kalmykova et al., 2005, Roy et al., 2007, Aravin et al., 2004, Blumenstiel et al., 2008, Malone et al., 2009, Shpiz et al., 2009, Cross and Simmons, 2008, Patil and Kai, 2010, Olivieri et al., 2010, Seong et al., 2011, Handler et al., 2011) Spindle E (Klenov et al., 2003, Bhadra et al., 2007) spindle E (Xing et al., 2007, Specchia et al., 2010) Spindle-E (Carmi, 2006, Lei et al., 2008, Kawamura et al., 2008, Szakmary et al., 2009, Janic et al., 2010) SpindleE (Chambeyron et al., 2008) Spindle-E (homeless) (Berg, 2005)
Secondary FlyBase IDs
	FBgn0004883
References ( 162 )
Generate a list of	All references relating to this gene ( 162 )
List References by type	Research paper  ( 82 ) Supplementary material  ( 3 ) Review  ( 35 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 22 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Conference report  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 2 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 ) Book  ( 1 )
Recent research papers ( 18 )
	Handler et al., 2011, EMBO J. 30(19): 3977--3993 A systematic analysis of Drosophila TUDOR domain-containing proteins identifies Vreteno and the Tdrd12 family as essential primary piRNA pathway factors. [FBrf0216344] Kibanov et al., 2011, Mol. Biol. Cell 22(18): 3410--3419 A novel organelle, the piNG-body, in the nuage of Drosophila male germ cells is associated with piRNA-mediated gene silencing. [FBrf0215572] Ni et al., 2011, Nat. Methods 8(5): 405--407 A genome-scale shRNA resource for transgenic RNAi in Drosophila. [FBrf0213581] Pek and Kai, 2011, Proc. Natl. Acad. Sci. U.S.A. 108(29): 12007--12012 DEAD-box RNA helicase Belle/DDX3 and the RNA interference pathway promote mitotic chromosome segregation. [FBrf0214405] Pek and Kai, 2011, Curr. Biol. 21(1): 39--44 A role for vasa in regulating mitotic chromosome condensation in Drosophila. [FBrf0212718] Roy et al., 2011, Mol. Genet. Genomics 285(2): 113--123 Lack of the Drosophila BEAF insulator proteins alters regulation of genes in the Antennapedia complex. [FBrf0212912] Seong et al., 2011, Cell 145(7): 1049--1061 Inheritance of Stress-Induced, ATF-2-Dependent Epigenetic Change. [FBrf0214019] Sharma et al., 2011, Chemosphere 82(3): 370--376 Transcriptome analysis provides insights for understanding the adverse effects of endosulfan in Drosophila melanogaster. [FBrf0212610] Shpiz et al., 2011, Nucleic Acids Res. 39(20): 8703--8711 Mechanism of the piRNA-mediated silencing of Drosophila telomeric retrotransposons. [FBrf0216525] Janic et al., 2010, Science 330(6012): 1824--1827 Ectopic expression of germline genes drives malignant brain tumor growth in Drosophila. [FBrf0212713] Moshkovich and Lei, 2010, PLoS Genet. 6(3): e1000880 HP1 Recruitment in the Absence of Argonaute Proteins in Drosophila. [FBrf0210291] Nagao et al., 2010, RNA 16(12): 2503--2515 Biogenesis pathways of piRNAs loaded onto AGO3 in the Drosophila testis. [FBrf0212366] Olivieri et al., 2010, EMBO J. 29(19): 3301--3317 An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila. [FBrf0212004] Patil and Kai, 2010, Curr. Biol. 20(8): 724--730 Repression of Retroelements in Drosophila Germline via piRNA Pathway by the Tudor Domain Protein Tejas. [FBrf0211166] Rouget et al., 2010, Nature 467(7319): 1128--1132 Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo. [FBrf0212183] Saito et al., 2010, Genes Dev. 24(22): 2493--2498 Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. [FBrf0212297] Specchia et al., 2010, Nature 463(7281): 662--665 Hsp90 prevents phenotypic variation by suppressing the mutagenic activity of transposons. [FBrf0209921] Stefanov et al., 2010, Mol. Genet. Genomics 284(5): 373--381 Amplification of "defective" retrotransposon gtwin in D. melanogaster strain carrying large complex chromosomal aberration. [FBrf0212070] Show all research papers ...
Recent reviews ( 1 )
	Arkov and Ramos, 2010, Trends Cell Biol. 20(8): 482--490 Building RNA-protein granules: insight from the germline. [FBrf0212430] Show all reviews ...