The gene spindle E is referred to in FlyBase by the symbol Dmel\spn-E (CG3158, FBgn0003483). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: helicase activity; ATP-dependent RNA helicase activity. There is experimental evidence for 16 unique biological process terms, many of which group under: cellular component organization or biogenesis; biological regulation; single-organism developmental process; localization; multicellular organism reproduction; anterior/posterior axis specification; gene silencing; dorsal/ventral axis specification; germarium-derived oocyte differentiation; negative regulation of cellular biosynthetic process; regulation of developmental process; karyosome formation; positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; cell cycle; polarity specification of dorsal/ventral axis. 35 alleles are reported. The phenotypes of these alleles are annotated with: germline cyst; P granule; oocyte; karyosome; spermatocyte; egg chamber; microtubule; dorsal appendage; synaptonemal complex. It has one annotated transcript and one annotated polypeptide. Protein features are: DNA/RNA helicase, DEAD/DEAH box type, N-terminal; Helicase, C-terminal; Helicase, superfamily 1/2, ATP-binding domain; Helicase-associated domain; Tudor domain; Zinc finger, C2H2; Zinc finger, C2H2-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1636886_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of spn-E. Gene sequence location is 3R:11663159..11670138.
User Contributed Data
External Summaries
Phenotypic Description from the Red Book (Lindsley
& Zimm 1992)
Gene/Allele symbols may differ
from current usage
spn-E: spindle E
Maternal-effect lethal. Similar to spn-A.
Recent Updates
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spn-E transcripts are expressed predominantly in ovaries but small amounts are detected in total male and female carcass RNA. In the germarium, expression is limited to the germline cells. Expression decreases through oogenesis stages S4/S5, increases during S8 and peaks in nurse cells at stage S10. No transcript is detected in the oocyte until stage S11 when it is deposited from the nurse cells. spn-E transcripts are uniformly distributed in early embryos.
Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary.
[download data (TSV)]
Guide to FlyAtlas expression level colors
No expression (0 - 9.999)
Low expression (10 - 99.999)
Moderate expression (100 - 499.999)
High level expression (500 - 999.999)
Very high expression (>999.999)
Linear, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
49.15
Larval Midgut
8.2
Larval Hindgut
8.9
Larval Malpighian Tubules
5.3
Larval Fat Body
53.5
Larval Salivary Gland
13.3
Larval Trachea
12.15
Larval Carcass
12.7
Adult Head
10.1
Adult Eye
33.575
Adult Brain
4.4
Adult Thoracic-Abdominal Ganglion
5.7
Adult Crop
5.9
Adult Midgut
8.1
Adult Hindgut
5.3
Adult Malpighian Tubules
5.4
Adult Fat Body
11.2
Adult Salivary Gland
14.1
Adult Heart
6.525
Adult VirginFemale Spermatheca
6.5
Adult InseminatedFemale Spermatheca
8.6
Adult Ovary
466.7
Adult Testis
66.6
Adult Male Accessory Gland
13.2
Adult Carcass
17.6
Expression Level Scale
None
Low
Moderate
High
Linear, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
49.15
Larval Midgut
8.2
Larval Hindgut
8.9
Larval Malpighian Tubules
5.3
Larval Fat Body
53.5
Larval Salivary Gland
13.3
Larval Trachea
12.15
Larval Carcass
12.7
Adult Head
10.1
Adult Eye
33.575
Adult Brain
4.4
Adult Thoracic-Abdominal Ganglion
5.7
Adult Crop
5.9
Adult Midgut
8.1
Adult Hindgut
5.3
Adult Malpighian Tubules
5.4
Adult Fat Body
11.2
Adult Salivary Gland
14.1
Adult Heart
6.525
Adult VirginFemale Spermatheca
6.5
Adult InseminatedFemale Spermatheca
8.6
Adult Ovary
466.7
Adult Testis
66.6
Adult Male Accessory Gland
13.2
Adult Carcass
17.6
Expression Level Scale
None
Low
Moderate
High
Linear, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
49.15
Larval Midgut
8.2
Larval Hindgut
8.9
Larval Malpighian Tubules
5.3
Larval Fat Body
53.5
Larval Salivary Gland
13.3
Larval Trachea
12.15
Larval Carcass
12.7
Adult Head
10.1
Adult Eye
33.575
Adult Brain
4.4
Adult Thoracic-Abdominal Ganglion
5.7
Adult Crop
5.9
Adult Midgut
8.1
Adult Hindgut
5.3
Adult Malpighian Tubules
5.4
Adult Fat Body
11.2
Adult Salivary Gland
14.1
Adult Heart
6.525
Adult VirginFemale Spermatheca
6.5
Adult InseminatedFemale Spermatheca
8.6
Adult Ovary
466.7
Adult Testis
66.6
Adult Male Accessory Gland
13.2
Adult Carcass
17.6
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
49.15
Larval Midgut
8.2
Larval Hindgut
8.9
Larval Malpighian Tubules
5.3
Larval Fat Body
53.5
Larval Salivary Gland
13.3
Larval Trachea
12.15
Larval Carcass
12.7
Adult Head
10.1
Adult Eye
33.575
Adult Brain
4.4
Adult Thoracic-Abdominal Ganglion
5.7
Adult Crop
5.9
Adult Midgut
8.1
Adult Hindgut
5.3
Adult Malpighian Tubules
5.4
Adult Fat Body
11.2
Adult Salivary Gland
14.1
Adult Heart
6.525
Adult VirginFemale Spermatheca
6.5
Adult InseminatedFemale Spermatheca
8.6
Adult Ovary
466.7
Adult Testis
66.6
Adult Male Accessory Gland
13.2
Adult Carcass
17.6
Expression Level Scale
Very high
log, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
49.15
Larval Midgut
8.2
Larval Hindgut
8.9
Larval Malpighian Tubules
5.3
Larval Fat Body
53.5
Larval Salivary Gland
13.3
Larval Trachea
12.15
Larval Carcass
12.7
Adult Head
10.1
Adult Eye
33.575
Adult Brain
4.4
Adult Thoracic-Abdominal Ganglion
5.7
Adult Crop
5.9
Adult Midgut
8.1
Adult Hindgut
5.3
Adult Malpighian Tubules
5.4
Adult Fat Body
11.2
Adult Salivary Gland
14.1
Adult Heart
6.525
Adult VirginFemale Spermatheca
6.5
Adult InseminatedFemale Spermatheca
8.6
Adult Ovary
466.7
Adult Testis
66.6
Adult Male Accessory Gland
13.2
Adult Carcass
17.6
Expression Level Scale
None
Low
Moderate
High
log, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
49.15
Larval Midgut
8.2
Larval Hindgut
8.9
Larval Malpighian Tubules
5.3
Larval Fat Body
53.5
Larval Salivary Gland
13.3
Larval Trachea
12.15
Larval Carcass
12.7
Adult Head
10.1
Adult Eye
33.575
Adult Brain
4.4
Adult Thoracic-Abdominal Ganglion
5.7
Adult Crop
5.9
Adult Midgut
8.1
Adult Hindgut
5.3
Adult Malpighian Tubules
5.4
Adult Fat Body
11.2
Adult Salivary Gland
14.1
Adult Heart
6.525
Adult VirginFemale Spermatheca
6.5
Adult InseminatedFemale Spermatheca
8.6
Adult Ovary
466.7
Adult Testis
66.6
Adult Male Accessory Gland
13.2
Adult Carcass
17.6
Expression Level Scale
None
Low
Moderate
High
log, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
49.15
Larval Midgut
8.2
Larval Hindgut
8.9
Larval Malpighian Tubules
5.3
Larval Fat Body
53.5
Larval Salivary Gland
13.3
Larval Trachea
12.15
Larval Carcass
12.7
Adult Head
10.1
Adult Eye
33.575
Adult Brain
4.4
Adult Thoracic-Abdominal Ganglion
5.7
Adult Crop
5.9
Adult Midgut
8.1
Adult Hindgut
5.3
Adult Malpighian Tubules
5.4
Adult Fat Body
11.2
Adult Salivary Gland
14.1
Adult Heart
6.525
Adult VirginFemale Spermatheca
6.5
Adult InseminatedFemale Spermatheca
8.6
Adult Ovary
466.7
Adult Testis
66.6
Adult Male Accessory Gland
13.2
Adult Carcass
17.6
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
49.15
Larval Midgut
8.2
Larval Hindgut
8.9
Larval Malpighian Tubules
5.3
Larval Fat Body
53.5
Larval Salivary Gland
13.3
Larval Trachea
12.15
Larval Carcass
12.7
Adult Head
10.1
Adult Eye
33.575
Adult Brain
4.4
Adult Thoracic-Abdominal Ganglion
5.7
Adult Crop
5.9
Adult Midgut
8.1
Adult Hindgut
5.3
Adult Malpighian Tubules
5.4
Adult Fat Body
11.2
Adult Salivary Gland
14.1
Adult Heart
6.525
Adult VirginFemale Spermatheca
6.5
Adult InseminatedFemale Spermatheca
8.6
Adult Ovary
466.7
Adult Testis
66.6
Adult Male Accessory Gland
13.2
Adult Carcass
17.6
Expression Level Scale
None
Low
Moderate
High
Very high
Heatmap
Tissue
Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass
FlyAtlas Organ/Tissue Expression, larval vs. adult
Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages.
[download data (TSV)]
Please Note FlyBase no
longer curates genomic clone accessions so this list
may not be complete
cDNA Clones ( 33 )
Please Note
This section lists
cDNAs and ESTs that fall within the genomic extent
of the gene model, which may include cDNAs and ESTs
of genes within introns, or of overlapping genes.
Please see GBrowse for alignment of the cDNAs and ESTs
to the gene model.
spn-E mutations have a dramatic effect on the distribution of Su(var)205 protein. Instead of the Su(var)205 protein being localised to pericentric heterochromatin and the fourth chromosome it is distributed across the whole of the polytene chromosomes. There is a reduction in the levels of the methyl-K9 form of His3.
Identification: in a germline clone screen for mutants that are defective in localisation of an Avic\GFP-stau marker in living oocytes. 6 alleles of spn-E have been identified in the screen.
Analysis of mutants of the 5 spindle genes (spn-A, spn-B, mus301, spn-D and spn-E) reveals that the group of genes is required for each of the symmetry-breaking steps that generate polarity during axis formation. spn-E is required for the localisation of fs(1)K10, bcd and osk mRNAs, spn-A. spn-B, mus301 and spn-D play an indirect role in this process.
spn-E gene product is necessary for the correct localisation of several RNAs that are involved in anteroposterior and dorsoventral axis formation during oogenesis.
The tudor domain protein Kumo is required to assemble the nuage and to generate germline piRNAs in Drosophila. [FBrf0217541]
Hodgetts et al., 2012, Genome 55(4): 312--326
An intact RNA interference pathway is required for expression of the mutant wing phenotype of vg(21-3), a P-element-induced allele of the vestigial gene in Drosophila. [FBrf0217920]
Lee and Langley, 2012, Genetics 192(4): 1411--1432
Long-term and short-term evolutionary impacts of transposable elements on Drosophila. [FBrf0220133]
The Cochaperone Shutdown Defines a Group of Biogenesis Factors Essential for All piRNA Populations in Drosophila. [FBrf0219572]
van Bergeijk et al., 2012, PLoS ONE 7(5): e36539
Genome-Wide Expression Analysis Identifies a Modulator of Ionizing Radiation-Induced p53-Independent Apoptosis in Drosophila melanogaster. [FBrf0218467]
Castillo et al., 2011, BMC Evol. Biol. 11: 258
Molecular evolution under increasing transposable element burden in Drosophila: A speed limit on the evolutionary arms race. [FBrf0216257]
Handler et al., 2011, EMBO J. 30(19): 3977--3993
A systematic analysis of Drosophila TUDOR domain-containing proteins identifies Vreteno and the Tdrd12 family as essential primary piRNA pathway factors. [FBrf0216344]
Kibanov et al., 2011, Mol. Biol. Cell 22(18): 3410--3419
A novel organelle, the piNG-body, in the nuage of Drosophila male germ cells is associated with piRNA-mediated gene silencing. [FBrf0215572]
Klenov et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(46): 18760--18765
Separation of stem cell maintenance and transposon silencing functions of Piwi protein. [FBrf0216776]
Kolaczkowski et al., 2011, Mol. Biol. Evol. 28(2): 1033--1042
Recurrent adaptation in RNA interference genes across the Drosophila phylogeny. [FBrf0212798]
Ni et al., 2011, Nat. Methods 8(5): 405--407
A genome-scale shRNA resource for transgenic RNAi in Drosophila. [FBrf0213581]
Pek and Kai, 2011, Proc. Natl. Acad. Sci. U.S.A. 108(29): 12007--12012
DEAD-box RNA helicase Belle/DDX3 and the RNA interference pathway promote mitotic chromosome segregation. [FBrf0214405]
Pek and Kai, 2011, Curr. Biol. 21(1): 39--44
A role for vasa in regulating mitotic chromosome condensation in Drosophila. [FBrf0212718]
Roy et al., 2011, Mol. Genet. Genomics 285(2): 113--123
Lack of the Drosophila BEAF insulator proteins alters regulation of genes in the Antennapedia complex. [FBrf0212912]
Seong et al., 2011, Cell 145(7): 1049--1061
Inheritance of Stress-Induced, ATF-2-Dependent Epigenetic Change. [FBrf0214019]
Sharma et al., 2011, Chemosphere 82(3): 370--376
Transcriptome analysis provides insights for understanding the adverse effects of endosulfan in Drosophila melanogaster. [FBrf0212610]
Shpiz et al., 2011, Nucleic Acids Res. 39(20): 8703--8711
Mechanism of the piRNA-mediated silencing of Drosophila telomeric retrotransposons. [FBrf0216525]
Tchurikov and Kretova, 2011, PLoS ONE 6(7): e21882
Both piRNA and siRNA Pathways Are Silencing Transcripts of the Suffix Element in the Drosophila melanogaster Germline and Somatic Cells. [FBrf0214474]