A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Gene Dmel\spn-E

General Information
SymbolDmel\spn-ESpeciesD. melanogaster
Namespindle EAnnotation symbolCG3158
Feature typeprotein_coding_geneFlyBase IDFBgn0003483
Gene Model StatusCurrent Stock availability 13 publicly available
Also Known Ashls, spnE
Genomic Location
Chromosome (arm)3RRecombination map3-62
Cytogenetic map89A5-89A5Sequence location3R:11,663,159..11,670,138 [+]

Genomic Maps

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modENCODE GBrowse
detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene spindle E is referred to in FlyBase by the symbol Dmel\spn-E (CG3158, FBgn0003483). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: helicase activity; ATP-dependent RNA helicase activity. There is experimental evidence for 15 unique biological process terms, many of which group under: cellular component organization or biogenesis; cellular process involved in reproduction; multicellular organismal reproductive process; biological regulation; anatomical structure development; localization; anterior/posterior axis specification; dorsal/ventral axis specification; regulation of cellular macromolecule biosynthetic process; positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; cell cycle; polarity specification of dorsal/ventral axis; regulation of multicellular organismal development. 34 alleles are reported. The phenotypes of these alleles are annotated with: germline cyst; P granule; oocyte; karyosome; spermatocyte; egg chamber; microtubule; dorsal appendage; synaptonemal complex. It has one annotated transcript and one annotated polypeptide. Protein features are: DEAD-like helicase; DNA/RNA helicase, DEAD/DEAH box type, N-terminal; Helicase, C-terminal; Helicase-associated domain; Maternal tudor protein; Tudor domain; Tudor subgroup; Zinc finger, C2H2; Zinc finger, C2H2-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1636886_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of spn-E. Gene sequence location is 3R:11663159..11670138.

External Summaries
hide Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage
spn-E: spindle E
Maternal-effect lethal. Similar to spn-A.
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2011_10
References
FB2012_01
References
Controlled Vocabulary Terms
Sequence features
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
89A5-89A5  
Limits computationally determined from genome sequence between P{PZ}l(3)0653606536 and P{PZ}blp01618  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
89B1-89B2  
(determined by in situ hybridisation)  
89A5-89A6  
(determined by in situ hybridisation)  
89A5-89A6  
(determined by in situ hybridisation)  
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
hide Gene Model & Products
Please see the GBrowse view of Dmel\spn-E for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
detailed view FBtr0300570 FBtr0300571 FBtr0309604 FBtr0309604 FBtr0083191 FBtr0083183 FBtr0083190 FBtr0083189 FBpp0289797 FBpp0289798 FBpp0082645 FBpp0082637 FBpp0082644 FBpp0082643 FBti0128452 FBti0048467 FBti0037718 FBti0009068_1 FBti0009068_2 FBti0040897 FBti0067085 FBti0048302 FBti0126109_2 FBti0126109_1 FBti0046073 FBti0126110_2 FBti0126110_1
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
FBtr0083183
  4661
  1434
Additional Transcript Data & Comments
Reported size (kB)
4.6 (northern blot)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
FBpp0082637  
164.5  
1434  
7.22  
Additional Polypeptide Data & Comments
Reported size (kDa)
1441 (aa); 155 (kD predicted)
Comments
External Data
Linkouts
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
hide Mapped Features
Mapped Features have been reorganized, please see this article for details.
Additional mapped features and mutations can be found on GBrowse or related reports.
Type
Symbol & Location
Additional Notes
References
hide External Data
Linkouts
Crossreferences
hide Expression Data
hideTranscript Expression
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
spn-E transcripts are expressed predominantly in ovaries but small amounts are detected in total male and female carcass RNA. In the germarium, expression is limited to the germline cells. Expression decreases through oogenesis stages S4/S5, increases during S8 and peaks in nurse cells at stage S10. No transcript is detected in the oocyte until stage S11 when it is deposited from the nurse cells. spn-E transcripts are uniformly distributed in early embryos.
Marker for
Subcellular Localization
CV Term
Notes
hidePolypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
hide High-Throughput Expression Data
or
Untitled Document detailed view ND23-RA spn-E-RA Pbp45-RA CG31150-RA
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

modENCODE Temporal Expression Data for FBgn0003483


   Styles
Linear
Logarithmic
Heatmap
   Scales
max expr for FBgn0003483
Very low expression bin max
Moderate expression bin max
High expression bin max
Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages.
[download data (TSV)]

Guide to modENCODE expression level colors
 
No expression (0 - 0)
 
Extremely low expression (1 - 10)
 
Very low expression (11 - 100)
 
Low expression (101 - 400)
 
Moderate expression (401 - 1400)
 
Moderately high expression (1401 - 4000)
 
High expression (4001 - 10000)
 
Very high expression (10001 - 100000)
 
Extremely high expression (100001 - 2000000)

Linear, scaled to maximum FBgn0003483 expression level
Developmental Stage   Expression Level
embryo 00-02hr
 
 2962
embryo 02-04hr
 
 1079
embryo 04-06hr
 
 318
embryo 06-08hr
 
 143
embryo 08-10hr
 
 188
embryo 10-12hr
 
 99
embryo 12-14hr
 
 133
embryo 14-16hr
 
 160
embryo 16-18hr
 
 129
embryo 18-20hr
 
 75
embryo 20-22hr
 
 177
embryo 22-24hr
 
 180
larva L1
 
 52
larva L2
 
 54
larva L3 12hr old
 
 47
larva L3 puffstage 1-2
 
 64
larva L3 puffstage 3-6
 
 96
larva L3 puffstage 7-9
 
 121
white prepupae new
 
 128
white prepupae 12hr
 
 195
white prepupae 24hr
 
 167
pupae 2d postWPP
 
 123
pupae 3d postWPP
 
 95
pupae 4d postWPP
 
 150
adult male 01day
 
 117
adult male 05day
 
 179
adult male 30day
 
 179
adult female 01day
 
 966
adult female 05day
 
 1882
adult female 30day
 
 1783
Expression Level Scale
 None 
 Extremely low 
 Very low 
 Low 
 Moderate 
 Moderately high 
Linear, scaled to Very low expression
Developmental Stage   Expression Level
embryo 00-02hr
 (2962)
embryo 02-04hr
 (1079)
embryo 04-06hr
 (318)
embryo 06-08hr
 (143)
embryo 08-10hr
 (188)
embryo 10-12hr
 
 99
embryo 12-14hr
 (133)
embryo 14-16hr
 (160)
embryo 16-18hr
 (129)
embryo 18-20hr
 
 75
embryo 20-22hr
 (177)
embryo 22-24hr
 (180)
larva L1
 
 52
larva L2
 
 54
larva L3 12hr old
 
 47
larva L3 puffstage 1-2
 
 64
larva L3 puffstage 3-6
 
 96
larva L3 puffstage 7-9
 (121)
white prepupae new
 (128)
white prepupae 12hr
 (195)
white prepupae 24hr
 (167)
pupae 2d postWPP
 (123)
pupae 3d postWPP
 
 95
pupae 4d postWPP
 (150)
adult male 01day
 (117)
adult male 05day
 (179)
adult male 30day
 (179)
adult female 01day
 (966)
adult female 05day
 (1882)
adult female 30day
 (1783)
Expression Level Scale
 None 
 Extremely low 
 Very low 
 Low 
Linear, scaled to Moderate expression
Developmental Stage   Expression Level
embryo 00-02hr
 (2962)
embryo 02-04hr
 
 1079
embryo 04-06hr
 
 318
embryo 06-08hr
 
 143
embryo 08-10hr
 
 188
embryo 10-12hr
 
 99
embryo 12-14hr
 
 133
embryo 14-16hr
 
 160
embryo 16-18hr
 
 129
embryo 18-20hr
 
 75
embryo 20-22hr
 
 177
embryo 22-24hr
 
 180
larva L1
 
 52
larva L2
 
 54
larva L3 12hr old
 
 47
larva L3 puffstage 1-2
 
 64
larva L3 puffstage 3-6
 
 96
larva L3 puffstage 7-9
 
 121
white prepupae new
 
 128
white prepupae 12hr
 
 195
white prepupae 24hr
 
 167
pupae 2d postWPP
 
 123
pupae 3d postWPP
 
 95
pupae 4d postWPP
 
 150
adult male 01day
 
 117
adult male 05day
 
 179
adult male 30day
 
 179
adult female 01day
 
 966
adult female 05day
 (1882)
adult female 30day
 (1783)
Expression Level Scale
 None 
 Extremely low 
 Very low 
 Low 
 Moderate 
 Moderately high 
Linear, scaled to High expression
Developmental Stage   Expression Level
embryo 00-02hr
 
 2962
embryo 02-04hr
 
 1079
embryo 04-06hr
 
 318
embryo 06-08hr
 
 143
embryo 08-10hr
 
 188
embryo 10-12hr
 
 99
embryo 12-14hr
 
 133
embryo 14-16hr
 
 160
embryo 16-18hr
 
 129
embryo 18-20hr
 
 75
embryo 20-22hr
 
 177
embryo 22-24hr
 
 180
larva L1
 
 52
larva L2
 
 54
larva L3 12hr old
 
 47
larva L3 puffstage 1-2
 
 64
larva L3 puffstage 3-6
 
 96
larva L3 puffstage 7-9
 
 121
white prepupae new
 
 128
white prepupae 12hr
 
 195
white prepupae 24hr
 
 167
pupae 2d postWPP
 
 123
pupae 3d postWPP
 
 95
pupae 4d postWPP
 
 150
adult male 01day
 
 117
adult male 05day
 
 179
adult male 30day
 
 179
adult female 01day
 
 966
adult female 05day
 
 1882
adult female 30day
 
 1783
Expression Level Scale
 None 
 Extremely low 
 Very low 
 Low 
 Moderate 
 Moderately high 
 High 
 Very high 
Linear, scaled to Extremely high expression
Developmental Stage   Expression Level
embryo 00-02hr
 
 2962
embryo 02-04hr
 
 1079
embryo 04-06hr
 
 318
embryo 06-08hr
 
 143
embryo 08-10hr
 
 188
embryo 10-12hr
 
 99
embryo 12-14hr
 
 133
embryo 14-16hr
 
 160
embryo 16-18hr
 
 129
embryo 18-20hr
 
 75
embryo 20-22hr
 
 177
embryo 22-24hr
 
 180
larva L1
 
 52
larva L2
 
 54
larva L3 12hr old
 
 47
larva L3 puffstage 1-2
 
 64
larva L3 puffstage 3-6
 
 96
larva L3 puffstage 7-9
 
 121
white prepupae new
 
 128
white prepupae 12hr
 
 195
white prepupae 24hr
 
 167
pupae 2d postWPP
 
 123
pupae 3d postWPP
 
 95
pupae 4d postWPP
 
 150
adult male 01day
 
 117
adult male 05day
 
 179
adult male 30day
 
 179
adult female 01day
 
 966
adult female 05day
 
 1882
adult female 30day
 
 1783
Expression Level Scale
 None 
 Extremely low 
 Very low 
 Low 
 Moderate 
 Moderately high 
 High 
 Very high 
 Extremely high 
log, scaled to maximum FBgn0003483 expression level
Developmental Stage   Expression Level
embryo 00-02hr
 
 2962
embryo 02-04hr
 
 1079
embryo 04-06hr
 
 318
embryo 06-08hr
 
 143
embryo 08-10hr
 
 188
embryo 10-12hr
 
 99
embryo 12-14hr
 
 133
embryo 14-16hr
 
 160
embryo 16-18hr
 
 129
embryo 18-20hr
 
 75
embryo 20-22hr
 
 177
embryo 22-24hr
 
 180
larva L1
 
 52
larva L2
 
 54
larva L3 12hr old
 
 47
larva L3 puffstage 1-2
 
 64
larva L3 puffstage 3-6
 
 96
larva L3 puffstage 7-9
 
 121
white prepupae new
 
 128
white prepupae 12hr
 
 195
white prepupae 24hr
 
 167
pupae 2d postWPP
 
 123
pupae 3d postWPP
 
 95
pupae 4d postWPP
 
 150
adult male 01day
 
 117
adult male 05day
 
 179
adult male 30day
 
 179
adult female 01day
 
 966
adult female 05day
 
 1882
adult female 30day
 
 1783
Expression Level Scale
 None 
 Extremely low 
 Very low 
 Low 
 Moderate 
 Moderately high 
 High 
log, scaled to Very low expression
Developmental Stage   Expression Level
embryo 00-02hr
 (2962)
embryo 02-04hr
 (1079)
embryo 04-06hr
 (318)
embryo 06-08hr
 143
embryo 08-10hr
 (188)
embryo 10-12hr
 
 99
embryo 12-14hr
 133
embryo 14-16hr
 (160)
embryo 16-18hr
 129
embryo 18-20hr
 
 75
embryo 20-22hr
 (177)
embryo 22-24hr
 (180)
larva L1
 
 52
larva L2
 
 54
larva L3 12hr old
 
 47
larva L3 puffstage 1-2
 
 64
larva L3 puffstage 3-6
 
 96
larva L3 puffstage 7-9
 121
white prepupae new
 128
white prepupae 12hr
 (195)
white prepupae 24hr
 (167)
pupae 2d postWPP
 123
pupae 3d postWPP
 
 95
pupae 4d postWPP
 (150)
adult male 01day
 117
adult male 05day
 (179)
adult male 30day
 (179)
adult female 01day
 (966)
adult female 05day
 (1882)
adult female 30day
 (1783)
Expression Level Scale
 None 
 Extremely low 
 Very low 
 Low 
log, scaled to Moderate expression
Developmental Stage   Expression Level
embryo 00-02hr
 (2962)
embryo 02-04hr
 
 1079
embryo 04-06hr
 
 318
embryo 06-08hr
 
 143
embryo 08-10hr
 
 188
embryo 10-12hr
 
 99
embryo 12-14hr
 
 133
embryo 14-16hr
 
 160
embryo 16-18hr
 
 129
embryo 18-20hr
 
 75
embryo 20-22hr
 
 177
embryo 22-24hr
 
 180
larva L1
 
 52
larva L2
 
 54
larva L3 12hr old
 
 47
larva L3 puffstage 1-2
 
 64
larva L3 puffstage 3-6
 
 96
larva L3 puffstage 7-9
 
 121
white prepupae new
 
 128
white prepupae 12hr
 
 195
white prepupae 24hr
 
 167
pupae 2d postWPP
 
 123
pupae 3d postWPP
 
 95
pupae 4d postWPP
 
 150
adult male 01day
 
 117
adult male 05day
 
 179
adult male 30day
 
 179
adult female 01day
 
 966
adult female 05day
 1882
adult female 30day
 1783
Expression Level Scale
 None 
 Extremely low 
 Very low 
 Low 
 Moderate 
 Moderately high 
log, scaled to High expression
Developmental Stage   Expression Level
embryo 00-02hr
 
 2962
embryo 02-04hr
 
 1079
embryo 04-06hr
 
 318
embryo 06-08hr
 
 143
embryo 08-10hr
 
 188
embryo 10-12hr
 
 99
embryo 12-14hr
 
 133
embryo 14-16hr
 
 160
embryo 16-18hr
 
 129
embryo 18-20hr
 
 75
embryo 20-22hr
 
 177
embryo 22-24hr
 
 180
larva L1
 
 52
larva L2
 
 54
larva L3 12hr old
 
 47
larva L3 puffstage 1-2
 
 64
larva L3 puffstage 3-6
 
 96
larva L3 puffstage 7-9
 
 121
white prepupae new
 
 128
white prepupae 12hr
 
 195
white prepupae 24hr
 
 167
pupae 2d postWPP
 
 123
pupae 3d postWPP
 
 95
pupae 4d postWPP
 
 150
adult male 01day
 
 117
adult male 05day
 
 179
adult male 30day
 
 179
adult female 01day
 
 966
adult female 05day
 
 1882
adult female 30day
 
 1783
Expression Level Scale
 None 
 Extremely low 
 Very low 
 Low 
 Moderate 
 Moderately high 
 High 
 Very high 
log, scaled to Extremely high expression
Developmental Stage   Expression Level
embryo 00-02hr
 
 2962
embryo 02-04hr
 
 1079
embryo 04-06hr
 
 318
embryo 06-08hr
 
 143
embryo 08-10hr
 
 188
embryo 10-12hr
 
 99
embryo 12-14hr
 
 133
embryo 14-16hr
 
 160
embryo 16-18hr
 
 129
embryo 18-20hr
 
 75
embryo 20-22hr
 
 177
embryo 22-24hr
 
 180
larva L1
 
 52
larva L2
 
 54
larva L3 12hr old
 
 47
larva L3 puffstage 1-2
 
 64
larva L3 puffstage 3-6
 
 96
larva L3 puffstage 7-9
 
 121
white prepupae new
 
 128
white prepupae 12hr
 
 195
white prepupae 24hr
 
 167
pupae 2d postWPP
 
 123
pupae 3d postWPP
 
 95
pupae 4d postWPP
 
 150
adult male 01day
 
 117
adult male 05day
 
 179
adult male 30day
 
 179
adult female 01day
 
 966
adult female 05day
 
 1882
adult female 30day
 
 1783
Expression Level Scale
 None 
 Extremely low 
 Very low 
 Low 
 Moderate 
 Moderately high 
 High 
 Very high 
 Extremely high 
Heatmap
Developmental Stage   Expression Level
embryo 00-02hr
 
 
embryo 02-04hr
 
 
embryo 04-06hr
 
 
embryo 06-08hr
 
 
embryo 08-10hr
 
 
embryo 10-12hr
 
 
embryo 12-14hr
 
 
embryo 14-16hr
 
 
embryo 16-18hr
 
 
embryo 18-20hr
 
 
embryo 20-22hr
 
 
embryo 22-24hr
 
 
larva L1
 
 
larva L2
 
 
larva L3 12hr old
 
 
larva L3 puffstage 1-2
 
 
larva L3 puffstage 3-6
 
 
larva L3 puffstage 7-9
 
 
white prepupae new
 
 
white prepupae 12hr
 
 
white prepupae 24hr
 
 
pupae 2d postWPP
 
 
pupae 3d postWPP
 
 
pupae 4d postWPP
 
 
adult male 01day
 
 
adult male 05day
 
 
adult male 30day
 
 
adult female 01day
 
 
adult female 05day
 
 
adult female 30day
 
 

FlyAtlas Anatomical Expression Data for FBgn0003483


   Styles
Linear
Logarithmic
Heatmap
Back-to-back
   Scales
max expr for FBgn0003483
Moderate expression bin max
High level expression bin max
Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary.
[download data (TSV)]

Guide to FlyAtlas expression level colors
 
No expression (0 - 9.999)
 
Low expression (10 - 99.999)
 
Moderate expression (100 - 499.999)
 
High level expression (500 - 999.999)
 
Very high expression (1000 - 25000)

Linear, scaled to maximum FBgn0003483 expression level
Tissue   Expression Level
Larval Central Nervous System
 
 49.15
Larval Midgut
 
 8.2
Larval Hindgut
 
 8.9
Larval Malpighian Tubules
 
 5.3
Larval Fat Body
 
 53.5
Larval Salivary Gland no informative data
Larval Trachea
 
 12.15
Larval Carcass
 
 12.7
Adult Head
 
 10.1
Adult Eye
 
 33.575
Adult Brain
 
 4.4
Adult Thoracic-Abdominal Ganglion
 
 5.7
Adult Crop
 
 5.9
Adult Midgut
 
 8.1
Adult Hindgut
 
 5.3
Adult Malpighian Tubules
 
 5.4
Adult Fat Body
 
 11.2
Adult Salivary Gland no informative data
Adult Heart
 
 6.525
Adult VirginFemale Spermatheca
 
 6.5
Adult InseminatedFemale Spermatheca
 
 8.6
Adult Ovary
 
 466.7
Adult Testis
 
 66.6
Adult Male Accessory Gland no informative data
Adult Carcass no informative data
Expression Level Scale
 None 
 Low 
 Moderate 
 High 
Linear, scaled to Moderate expression
Tissue   Expression Level
Larval Central Nervous System
 
 49.15
Larval Midgut
 
 8.2
Larval Hindgut
 
 8.9
Larval Malpighian Tubules
 
 5.3
Larval Fat Body
 
 53.5
Larval Salivary Gland no informative data
Larval Trachea
 
 12.15
Larval Carcass
 
 12.7
Adult Head
 
 10.1
Adult Eye
 
 33.575
Adult Brain
 
 4.4
Adult Thoracic-Abdominal Ganglion
 
 5.7
Adult Crop
 
 5.9
Adult Midgut
 
 8.1
Adult Hindgut
 
 5.3
Adult Malpighian Tubules
 
 5.4
Adult Fat Body
 
 11.2
Adult Salivary Gland no informative data
Adult Heart
 
 6.525
Adult VirginFemale Spermatheca
 
 6.5
Adult InseminatedFemale Spermatheca
 
 8.6
Adult Ovary
 
 466.7
Adult Testis
 
 66.6
Adult Male Accessory Gland no informative data
Adult Carcass no informative data
Expression Level Scale
 None 
 Low 
 Moderate 
 High 
Linear, scaled to High level expression
Tissue   Expression Level
Larval Central Nervous System
 
 49.15
Larval Midgut
 
 8.2
Larval Hindgut
 
 8.9
Larval Malpighian Tubules
 
 5.3
Larval Fat Body
 
 53.5
Larval Salivary Gland no informative data
Larval Trachea
 
 12.15
Larval Carcass
 
 12.7
Adult Head
 
 10.1
Adult Eye
 
 33.575
Adult Brain
 
 4.4
Adult Thoracic-Abdominal Ganglion
 
 5.7
Adult Crop
 
 5.9
Adult Midgut
 
 8.1
Adult Hindgut
 
 5.3
Adult Malpighian Tubules
 
 5.4
Adult Fat Body
 
 11.2
Adult Salivary Gland no informative data
Adult Heart
 
 6.525
Adult VirginFemale Spermatheca
 
 6.5
Adult InseminatedFemale Spermatheca
 
 8.6
Adult Ovary
 
 466.7
Adult Testis
 
 66.6
Adult Male Accessory Gland no informative data
Adult Carcass no informative data
Expression Level Scale
 None 
 Low 
 Moderate 
 High 
 Very high 
Linear, scaled to Very high expression
Tissue   Expression Level
Larval Central Nervous System
 
 49.15
Larval Midgut
 
 8.2
Larval Hindgut
 
 8.9
Larval Malpighian Tubules
 
 5.3
Larval Fat Body
 
 53.5
Larval Salivary Gland no informative data
Larval Trachea
 
 12.15
Larval Carcass
 
 12.7
Adult Head
 
 10.1
Adult Eye
 
 33.575
Adult Brain
 
 4.4
Adult Thoracic-Abdominal Ganglion
 
 5.7
Adult Crop
 
 5.9
Adult Midgut
 
 8.1
Adult Hindgut
 
 5.3
Adult Malpighian Tubules
 
 5.4
Adult Fat Body
 
 11.2
Adult Salivary Gland no informative data
Adult Heart
 
 6.525
Adult VirginFemale Spermatheca
 
 6.5
Adult InseminatedFemale Spermatheca
 
 8.6
Adult Ovary
 
 466.7
Adult Testis
 
 66.6
Adult Male Accessory Gland no informative data
Adult Carcass no informative data
Expression Level Scale
 None 
 Low 
 Moderate 
 High 
 Very high 
log, scaled to maximum FBgn0003483 expression level
Tissue   Expression Level
Larval Central Nervous System
 
 49.15
Larval Midgut
 
 8.2
Larval Hindgut
 
 8.9
Larval Malpighian Tubules
 
 5.3
Larval Fat Body
 
 53.5
Larval Salivary Gland no informative data
Larval Trachea
 
 12.15
Larval Carcass
 
 12.7
Adult Head
 
 10.1
Adult Eye
 
 33.575
Adult Brain
 
 4.4
Adult Thoracic-Abdominal Ganglion
 
 5.7
Adult Crop
 
 5.9
Adult Midgut
 
 8.1
Adult Hindgut
 
 5.3
Adult Malpighian Tubules
 
 5.4
Adult Fat Body
 
 11.2
Adult Salivary Gland no informative data
Adult Heart
 
 6.525
Adult VirginFemale Spermatheca
 
 6.5
Adult InseminatedFemale Spermatheca
 
 8.6
Adult Ovary
 
 466.7
Adult Testis
 
 66.6
Adult Male Accessory Gland no informative data
Adult Carcass no informative data
Expression Level Scale
 None 
 Low 
 Moderate 
 High 
log, scaled to Moderate expression
Tissue   Expression Level
Larval Central Nervous System
 
 49.15
Larval Midgut
 
 8.2
Larval Hindgut
 
 8.9
Larval Malpighian Tubules
 
 5.3
Larval Fat Body
 
 53.5
Larval Salivary Gland no informative data
Larval Trachea
 
 12.15
Larval Carcass
 
 12.7
Adult Head
 
 10.1
Adult Eye
 
 33.575
Adult Brain
 
 4.4
Adult Thoracic-Abdominal Ganglion
 
 5.7
Adult Crop
 
 5.9
Adult Midgut
 
 8.1
Adult Hindgut
 
 5.3
Adult Malpighian Tubules
 
 5.4
Adult Fat Body
 
 11.2
Adult Salivary Gland no informative data
Adult Heart
 
 6.525
Adult VirginFemale Spermatheca
 
 6.5
Adult InseminatedFemale Spermatheca
 
 8.6
Adult Ovary
 
 466.7
Adult Testis
 
 66.6
Adult Male Accessory Gland no informative data
Adult Carcass no informative data
Expression Level Scale
 None 
 Low 
 Moderate 
 High 
log, scaled to High level expression
Tissue   Expression Level
Larval Central Nervous System
 
 49.15
Larval Midgut
 
 8.2
Larval Hindgut
 
 8.9
Larval Malpighian Tubules
 
 5.3
Larval Fat Body
 
 53.5
Larval Salivary Gland no informative data
Larval Trachea
 
 12.15
Larval Carcass
 
 12.7
Adult Head
 
 10.1
Adult Eye
 
 33.575
Adult Brain
 
 4.4
Adult Thoracic-Abdominal Ganglion
 
 5.7
Adult Crop
 
 5.9
Adult Midgut
 
 8.1
Adult Hindgut
 
 5.3
Adult Malpighian Tubules
 
 5.4
Adult Fat Body
 
 11.2
Adult Salivary Gland no informative data
Adult Heart
 
 6.525
Adult VirginFemale Spermatheca
 
 6.5
Adult InseminatedFemale Spermatheca
 
 8.6
Adult Ovary
 
 466.7
Adult Testis
 
 66.6
Adult Male Accessory Gland no informative data
Adult Carcass no informative data
Expression Level Scale
 None 
 Low 
 Moderate 
 High 
 Very high 
log, scaled to Very high expression
Tissue   Expression Level
Larval Central Nervous System
 
 49.15
Larval Midgut
 
 8.2
Larval Hindgut
 
 8.9
Larval Malpighian Tubules
 
 5.3
Larval Fat Body
 
 53.5
Larval Salivary Gland no informative data
Larval Trachea
 
 12.15
Larval Carcass
 
 12.7
Adult Head
 
 10.1
Adult Eye
 
 33.575
Adult Brain
 
 4.4
Adult Thoracic-Abdominal Ganglion
 
 5.7
Adult Crop
 
 5.9
Adult Midgut
 
 8.1
Adult Hindgut
 
 5.3
Adult Malpighian Tubules
 
 5.4
Adult Fat Body
 
 11.2
Adult Salivary Gland no informative data
Adult Heart
 
 6.525
Adult VirginFemale Spermatheca
 
 6.5
Adult InseminatedFemale Spermatheca
 
 8.6
Adult Ovary
 
 466.7
Adult Testis
 
 66.6
Adult Male Accessory Gland no informative data
Adult Carcass no informative data
Expression Level Scale
 None 
 Low 
 Moderate 
 High 
 Very high 
Heatmap
Tissue   Expression Level
Larval Central Nervous System
 
 
Larval Midgut
 
 
Larval Hindgut
 
 
Larval Malpighian Tubules
 
 
Larval Fat Body
 
 
Larval Salivary Gland no informative data
Larval Trachea
 
 
Larval Carcass
 
 
Adult Head
 
 
Adult Eye
 
 
Adult Brain
 
 
Adult Thoracic-Abdominal Ganglion
 
 
Adult Crop
 
 
Adult Midgut
 
 
Adult Hindgut
 
 
Adult Malpighian Tubules
 
 
Adult Fat Body
 
 
Adult Salivary Gland no informative data
Adult Heart
 
 
Adult VirginFemale Spermatheca
 
 
Adult InseminatedFemale Spermatheca
 
 
Adult Ovary
 
 
Adult Testis
 
 
Adult Male Accessory Gland no informative data
Adult Carcass no informative data

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level Tissue Adult Expression Level
 
NA 
Head
 
 10.1
 
NA 
Eye
 
 33.575
 
NA 
Brain
 
 4.4
 
49.15 
Central Nervous System
 
 NA
 
NA 
Thoracic-Abdominal Ganglion
 
 5.7
 
NA 
Crop
 
 5.9
 
8.2 
Midgut
 
 8.1
 
8.9 
Hindgut
 
 5.3
 
5.3 
Malpighian Tubules
 
 5.4
 
53.5 
Fat Body
 
 11.2
 
no informative data 
Salivary Gland
 
 no informative data
 
NA 
Heart
 
 6.525
 
12.15 
Trachea
 
 NA
 
NA 
VirginFemale Spermatheca
 
 6.5
 
NA 
InseminatedFemale Spermatheca
 
 8.6
 
NA 
Ovary
 
 466.7
 
NA 
Testis
 
 66.6
 
NA 
Male Accessory Gland
 
 no informative data
 
12.7 
Carcass
 
 no informative data

modENCODE Temporal Expression Data (Graveley et al., 2011)
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
hide Expression Clusters
A cluster of genes with similar mRNA expression dynamics across development.
hide External Data & Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
hide Alleles & Phenotypes
hide Summary of Allele Phenotypes
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
meiosis & nuclear chromosome
meiosis & nuclear chromosome (with spn-E1)
meiosis & nuclear chromosome (with spn-E2)
meiosis & nuclear chromosome (with spn-Ehls-03987)
meiosis & nuclear chromosome (with spn-Ehls-Δ125)
meiosis & nuclear chromosome | male
hide Classical Alleles ( 25 )
For All Classical Alleles Show

Allele of spn-EClassMutagenStocksKnown lesion
spn-E1hypomorphic allele - genetic evidence2 --
spn-Ec007862 --
spn-Ec070431 --
spn-Ee034411 --
spn-Ehls-03987hypomorphic allele - genetic evidence1 Yes
spn-E167R33amorphic allele - genetic evidence0 --
spn-E100.37
0 --
spn-E125
0 --
spn-E167hypomorphic allele - genetic evidence0 --
spn-E167R180 --
spn-E2hypomorphic allele - genetic evidence0 --
spn-E2A9-140 --
spn-E4E2-140 --
spn-E7G2-50 --
spn-E8D4-110 --
spn-E9A2-170 --
spn-E9A9-180 --
spn-ED58
0 --
spn-EDE8
0 --
spn-Ee041030 --
spn-Ehls-E1
0 --
spn-Ehls-Δ125loss of function allele0 Yes
spn-Ehls-Δ1390 Yes
spn-Ehls-Δ157loss of function allele0 Yes
spn-Eunspecified
0 --
hide Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show

Allele of spn-EClassMutagenStocksKnown lesion
spn-EGD102992 Yes
spn-EGL002061 Yes
spn-EHMS001171 Yes
spn-EJF014051 Yes
spn-EKK1015251 Yes
spn-EdsRNA.cDa0 Yes
spn-EdsRNA.shRNA.Scer\UAS.P\T0 Yes
spn-ET:Uuuu\T70 Yes
spn-EtCH322-93D040 Yes
hide Aneuploid Aberrations
Disrupted in
Not disrupted in
hide Transgenic Constructs & Insertions
Transgenic Constructs
Type of construct
Name
Expression data
characterization construct
Insertions
Type of insertions
Name
Expression data
hide Gene Ontology: Function, Process & Cellular Component ( 29 unique terms )
hide Terms Based on Experimental Evidence ( 18 terms )
Molecular Function ( 0 terms)
Biological Process
CV term
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with fs(1)K10
Cellular Component
CV term
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
hide Terms Based on Predictions or Assertions ( 14 terms )
Molecular Function
CV term
References
inferred from sequence or structural similarity with InterPro:IPR001410
inferred from sequence or structural similarity
traceable author statement
Biological Process
CV term
References
non-traceable author statement
non-traceable author statement
traceable author statement
Cellular Component
CV term
References
non-traceable author statement
hide Sequence Ontology: Class of Gene
hide Interactions & Pathways
hide Summary of Physical Interactions
Protein-protein
Interacting group
Assay
References
hide Summary of Genetic Interactions
Interacts with
Please look at the allele data for full details of the genetic interactions
spn-E allele
Gene
References
hide External Data
Linkouts
BioGRID - A database of protein and genetic interactions
DroID - A comprehensive database of gene and protein interactions.
InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.
hide Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs
Linkouts
InParanoid A subset of ortholog calls from InParanoid.
OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs.
hide Stocks & Reagents
hide Stocks Listed in FlyBase ( 13 )
Bloomington
Harvard
VDRC
hide Genomic Clones ( 1 )
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
hide cDNA Clones ( 33 )
Please Note
This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
hide RNAi & Array Information
Linkouts
DRSC - Results from RNAi screens.
GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
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hide Etymology
Named "homeless" based on oocyte mislocalisation phenotype and RNA localisation phenotype.
hide Identification
hide Relationship to Other Genes
Source for database identity of
Source for identity of: spn-E CG3158
Source for database merge of
Additional comments
hide Other Comments
spn-E is required for the accumulation of repeat-associated small interfering RNAs (rasiRNAs).
piwi, aub and spn-E are suppressors of repeat-induced silencing, as seen at heterochromatic tandem arrays of insertions of the P{lacW} transposon.
spn-E mutations have a dramatic effect on the distribution of Su(var)205 protein. Instead of the Su(var)205 protein being localised to pericentric heterochromatin and the fourth chromosome it is distributed across the whole of the polytene chromosomes. There is a reduction in the levels of the methyl-K9 form of His3.
Identification: in a germline clone screen for mutants that are defective in localisation of an Avic\GFP-stau marker in living oocytes. 6 alleles of spn-E have been identified in the screen.
spn-E mutants block RNAi activation which normally occurs during egg maturation.
spn-E has a role in repressing the Ste locus and may have a role in repressing other repeated sequences.
Analysis of mutants of the 5 spindle genes (spn-A, spn-B, mus301, spn-D and spn-E) reveals that the group of genes is required for each of the symmetry-breaking steps that generate polarity during axis formation. spn-E is required for the localisation of fs(1)K10, bcd and osk mRNAs, spn-A. spn-B, mus301 and spn-D play an indirect role in this process.
spn-E gene product is necessary for the correct localisation of several RNAs that are involved in anteroposterior and dorsoventral axis formation during oogenesis.
hide External Crossreferences & Linkouts
Sequence Crossreferences
RefSeq (Transcripts)
RefSeq (Proteins)
Entrez Gene - A searchable database of RefSeq genes.
Other Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
Linkouts
BioGRID - A database of protein and genetic interactions
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results from RNAi screens.
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
InParanoid A subset of ortholog calls from InParanoid.
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.
modMine - Data generated by the modENCODE project.
OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs.
hide Synonyms & Secondary IDs ( 23 )
Reported As
Symbol Synonym
fs(1)hls
fs(3)hls
 
Name Synonym
female sterile(3)homeless
 
homeless/spindle-E
Spindle-E (homeless)
Secondary FlyBase IDs
  • FBgn0004883
hide References ( 162 )
Generate a list of
List References by type
hide Recent research papers ( 18 )
Handler et al., 2011, EMBO J. 30(19): 3977--3993
A systematic analysis of Drosophila TUDOR domain-containing proteins identifies Vreteno and the Tdrd12 family as essential primary piRNA pathway factors. [FBrf0216344]
Kibanov et al., 2011, Mol. Biol. Cell 22(18): 3410--3419
A novel organelle, the piNG-body, in the nuage of Drosophila male germ cells is associated with piRNA-mediated gene silencing. [FBrf0215572]
Ni et al., 2011, Nat. Methods 8(5): 405--407
A genome-scale shRNA resource for transgenic RNAi in Drosophila. [FBrf0213581]
Pek and Kai, 2011, Proc. Natl. Acad. Sci. U.S.A. 108(29): 12007--12012
DEAD-box RNA helicase Belle/DDX3 and the RNA interference pathway promote mitotic chromosome segregation. [FBrf0214405]
Pek and Kai, 2011, Curr. Biol. 21(1): 39--44
A role for vasa in regulating mitotic chromosome condensation in Drosophila. [FBrf0212718]
Roy et al., 2011, Mol. Genet. Genomics 285(2): 113--123
Lack of the Drosophila BEAF insulator proteins alters regulation of genes in the Antennapedia complex. [FBrf0212912]
Seong et al., 2011, Cell 145(7): 1049--1061
Inheritance of Stress-Induced, ATF-2-Dependent Epigenetic Change. [FBrf0214019]
Sharma et al., 2011, Chemosphere 82(3): 370--376
Transcriptome analysis provides insights for understanding the adverse effects of endosulfan in Drosophila melanogaster. [FBrf0212610]
Shpiz et al., 2011, Nucleic Acids Res. 39(20): 8703--8711
Mechanism of the piRNA-mediated silencing of Drosophila telomeric retrotransposons. [FBrf0216525]
Janic et al., 2010, Science 330(6012): 1824--1827
Ectopic expression of germline genes drives malignant brain tumor growth in Drosophila. [FBrf0212713]
Moshkovich and Lei, 2010, PLoS Genet. 6(3): e1000880
HP1 Recruitment in the Absence of Argonaute Proteins in Drosophila. [FBrf0210291]
Nagao et al., 2010, RNA 16(12): 2503--2515
Biogenesis pathways of piRNAs loaded onto AGO3 in the Drosophila testis. [FBrf0212366]
Olivieri et al., 2010, EMBO J. 29(19): 3301--3317
An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila. [FBrf0212004]
Patil and Kai, 2010, Curr. Biol. 20(8): 724--730
Repression of Retroelements in Drosophila Germline via piRNA Pathway by the Tudor Domain Protein Tejas. [FBrf0211166]
Rouget et al., 2010, Nature 467(7319): 1128--1132
Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo. [FBrf0212183]
Saito et al., 2010, Genes Dev. 24(22): 2493--2498
Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. [FBrf0212297]
Specchia et al., 2010, Nature 463(7281): 662--665
Hsp90 prevents phenotypic variation by suppressing the mutagenic activity of transposons. [FBrf0209921]
Stefanov et al., 2010, Mol. Genet. Genomics 284(5): 373--381
Amplification of "defective" retrotransposon gtwin in D. melanogaster strain carrying large complex chromosomal aberration. [FBrf0212070]
hide Recent reviews ( 1 )
Arkov and Ramos, 2010, Trends Cell Biol. 20(8): 482--490
Building RNA-protein granules: insight from the germline. [FBrf0212430]