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General Information
Symbol
Dmel\Btk29A
Species
D. melanogaster
Name
Btk family kinase at 29A
Annotation Symbol
CG8049
Feature Type
FlyBase ID
FBgn0003502
Gene Model Status
Stock Availability
Enzyme Name (EC)
Non-specific protein-tyrosine kinase (2.7.10.2)
Gene Snapshot
Btk family kinase at 29A (Btk29A) encodes a non-receptor tyrosine kinase with diverse roles including cellularization, morphogenesis, patterning and germ cell proliferation. [Date last reviewed: 2019-03-07]
Also Known As

Tec29, Dsrc28C, Tec29A, fic, dsrc29A

Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:8,258,373..8,301,079 [-]
Recombination map

2-31

RefSeq locus
NT_033779 REGION:8258373..8301079
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (30 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
inferred from mutant phenotype
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN001231474
(assigned by GO_Central )
Biological Process (22 terms)
Terms Based on Experimental Evidence (17 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from direct assay
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
non-traceable author statement
traceable author statement
inferred from electronic annotation with InterPro:IPR001562
(assigned by InterPro )
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN001231474
(assigned by GO_Central )
Cellular Component (5 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily. (P08630)
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate (2.7.10.2)
Summaries
Gene Group (FlyBase)
NON-RECEPTOR TYROSINE KINASES -
Non-Receptor Tyrosine kinases (nRTK) are cytoplasmic protein kinases that specifically phosphorylate tyrosine residues. nRTKs are involved in signalling cascades and possess targeting domains such as SH2 domains. (Adapted from FBrf0132098).
Protein Function (UniProtKB)
Required for proper ring canal development. Also required for the development of male genitalia and for adult survival.
(UniProt, P08630)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Src2
Src transcripts abundant in early embryos and in adult ovaries; also found in imaginal disks. Level declines in later embryos and is low in males and ovarectomized females. Inferred to be maternally acting gene whose product is required for early embryogenesis (Wadsworth et al.). Monoclonal antibodies detect two protein products of Src2, a doublet of 66 kd and one of 55 kd; the 55 kd polypeptide appears to initiate at the third methionine codon of the sequence; a faint 95 kd band appears to track the 66 kd band in developmental Northern blots. Monoclonal antibodies that differentiate between the two protein products show that they are differentially regulated. The 66 kd polypeptide first appears at the periphery of cells of the cellular blastoderm; this staining is transiently resolved into 13 or 14 segmental stripes plus uncharacterized staining in the head region in the fully extended germ band; no 66 kd protein detectable after 12 h of development. The 55 kd polypeptide first appears at 4-6 h and is widely distributed in the central and peripheral nervous systems. Larvae transformed with constructs that produce either the 66 or the 55 kd products under Hsp70 promoter action display cell-surface localization of the 66 kd protein and cytoplasmic localization of the 55 kd protein in third-instar salivary glands.
Summary (Interactive Fly)

protein-tyrosine kinase - necessary for maintaining the equilibrium between monomeric actin and filamentous actin during invagination of the salivary placodes - functions during oogenesis as a key downstream effector of Src64 during ring canal growth - targets β-catenin, which functions downstream of Wnt4 in escort cells to terminate Drosophila germ cell proliferation through up-regulation of expression

Gene Model and Products
Number of Transcripts
8
Number of Unique Polypeptides
4

Please see the JBrowse view of Dmel\Btk29A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.55

Gene model reviewed during 5.45

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.44

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0079618
4026
603
FBtr0079616
3420
786
FBtr0079615
3279
603
FBtr0079617
3674
786
FBtr0112836
4785
786
FBtr0112835
4992
603
FBtr0330692
3302
565
FBtr0330693
3826
464
Additional Transcript Data and Comments
Reported size (kB)

4.0, 3.0 (northern blot)

3.5 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0079238
66.8
603
8.80
FBpp0079236
87.4
786
9.39
FBpp0079235
66.8
603
8.80
FBpp0079237
87.4
786
9.39
FBpp0111749
87.4
786
9.39
FBpp0111748
66.8
603
8.80
FBpp0303535
62.4
565
8.74
FBpp0303536
52.9
464
8.60
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

603 aa isoforms: Btk29A-PA, Btk29A-PC, Btk29A-PF
786 aa isoforms: Btk29A-PB, Btk29A-PD, Btk29A-PE
Additional Polypeptide Data and Comments
Reported size (kDa)

786, 603 (aa)

590 (aa); 66 (kD)

Comments

The 55kD gene product of Btk29A differs from

the 66kD product by the absence of the 125 N-terminal amino acids. When

expressed in Sf9 cells via a recombinant baculovirus system it does not

exhibit detectable protein tyrosine-kinase activity. The p55 product lacks

an N-terminal myristylation signal.

In addition to the 66 and 55 kD Btk29A proteins, a 95 kD protein is observed on Western blots.

External Data
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Btk29A using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
dot blot
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
radioisotope in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Btk29A transcript is expressed in posterior spiracle cells, and their precursors, from embryonic stage 11 through the end of embryogenesis.

Type 1 and type 2 Btk29A transcripts are expressed in mutually exclusive patterns in the pupal and larval genital and nervous systems. Type 2 transcript is expressed in the male pupal genital disc along the edge of the male genital primordium facing the lumen, which is thought to give rise to main body of the male genitalia. In the larval and pupal CNS, type 2 transcript is detected in the mushroom body and antennal lobe, while type 1 transcript is detected in dispersed neurons and midline glial cells.

Type 1 and type 2 Btk29A transcripts are expressed in mutually exclusive patterns in the pupal and larval genital and nervous systems. Type 1 transcript expression in the male pupal genital disc is restricted to the anterior bulbus, which will give rise to the internal genitalia.In the larval and pupal CNS, type 2 transcript is detected in the mushroom body and antennal lobe, while type 1 transcript is detected in dispersed neurons and midline glial cells.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
epitope tag
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference

Comment: reference states 4-14 hr AEL

Comment: reference states >=4 hr AEL

Additional Descriptive Data

Btk29A protein is expressed in posterior spiracle cells, and their precursors, from embryonic stage 11 through the end of embryogenesis.

Type 2 protein is the predominant form in the female germline.

Btk29A protein is localized strongly to the cellularization front, and less strongly to the apico-lateral membrane, during cellularization of the embryo.

Btk29A protein is detected in egg chambers. It is detected in ring canals starting at region 2b of the germarium through the remainder of oogenesis.

The 55 kD Btk29A protein is first detected at 4-6 hr of embryogenesis, and persists through the rest of embryogenesis. It is likely that the 55 kD protein is the nervous-system-specific cytoplasmic form.

A reticular pattern of staining around the periphery of ectodermal cells is observed in cellular blastoderm embryos, and persists through early germ band extension. A transient (1 hr) 13-14 striped pattern of expression is observed at full germ band extension. After the striped pattern fades, Btk29A protein is observed in the central and peripheral nervous systems, and is no longer peripheral, but cytoplasmic.

Marker for
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Btk29A in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 39 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 35 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Btk29A
Transgenic constructs containing regulatory region of Btk29A
Deletions and Duplications ( 7 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
egg chamber & actin cytoskeleton | germ-line clone
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (23)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
10 of 15
No
Yes
9 of 15
No
Yes
8 of 15
No
Yes
 
3  
7 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2  
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1  
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (22)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
10 of 15
No
Yes
9 of 15
No
Yes
8 of 15
No
Yes
7 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (20)
9 of 13
Yes
Yes
8 of 13
No
Yes
7 of 13
No
Yes
5 of 13
No
Yes
3 of 13
No
Yes
3 of 13
No
Yes
2 of 13
No
No
2 of 13
No
Yes
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (20)
6 of 12
Yes
Yes
2 of 12
No
Yes
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (23)
7 of 15
Yes
Yes
7 of 15
Yes
Yes
6 of 15
No
Yes
6 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (7)
2 of 15
Yes
No
2 of 15
Yes
No
2 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (31)
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
No
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091903G3 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500SQ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00MF )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01J8 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0D46 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (13)
4 of 10
4 of 10
4 of 10
3 of 10
3 of 10
2 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 3 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Dmel gene
    Ortholog showing functional complementation
    Supporting References
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map

    2-31

    Cytogenetic map
    Sequence location
    2L:8,258,373..8,301,079 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    29A1-29A3
    Limits computationally determined from genome sequence between P{lacW}l(2)k16919k16919&P{lacW}Bsgk09030 and P{lacW}Btk29Ak00206&P{EP}Btk29AEP715
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    29A1-29A2
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (26)
    Genomic Clones (43)
    cDNA Clones (129)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of

    Source for merge of: Btk29A CG18355

    Additional comments
    Other Comments

    Btk29A is required non cell-autonomously for the invagination of the spiracular chamber during morphogenesis of the embryonic respiratory system.

    Btk29A plays a role in the cytoskeletal dynamics that occur during cellularization of the blastoderm.

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    Btk29A exerts pleiotropic functions in different developmental contexts in different tissues through the generation of distinct forms of protein products by means of alternative splicing.

    Btk29A is required for the development of the male genitalia and substrates required for adult survival.

    Btk29A is essential for head involution during embryogenesis and ring canal development during oogenesis.

    Phylogenetic analysis of the PTK family.

    Both the p55 and p66 products lack a N-terminal myristylation signal. 66kD gene product expressed in Sf9 cells via a recombinant baculovirus has protein tyrosine-kinase activity, but it does not autophosphorylate nor does it use classic 'src' substrates. 55kD gene product expressed in the same system does not exhibit detectable protein tyrosine-kinase activity. A deletion derivative of the 66kD gene product lacking the SH3 and SH2 domains also failed to phosphorylate Sf9 cell proteins.

    The mutant stocks studied which show neoplastic growth have structural rearrangements in the Btk29A gene.

    Examination of the expression of Btk29A two major proteins, p66 and p55, suggests that Btk29A plays a role in the development of numerous embryonic and larval tissues.

    Structural and biochemical evidence suggest the gene encodes a protein tyrosine kinase.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 107 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DPiM - Drosophila Protein interaction map
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (39)
    Reported As
    Symbol Synonym
    C-src2
    C-src4
    CG18355
    S13
    Src2
    src2
    src4
    Name Synonyms
    Btk family kinase at 29A
    Src oncogene 29A
    Secondary FlyBase IDs
    • FBgn0032011
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (214)