Gene Dmel\Btk29A

General Information
Symbol	Dmel\Btk29A	Species	D. melanogaster
Name	Btk family kinase at 29A	Annotation symbol	CG8049
Feature type	protein_coding_gene	FlyBase ID	FBgn0003502
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	29A1-29A3	Sequence location	2L:8,259,504..8,301,072 [-]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene Btk family kinase at 29A is referred to in FlyBase by the symbol Btk29A (CG8049, FBgn0003502). It has the cytological map location 29A1-29A3. Its sequence location is 2L:8259504..8301072. Its molecular function is described as: protein-tyrosine kinase activity; ATP binding; protein binding. It is involved in the biological processes described with 20 unique terms, many of which group under: anatomical structure development; gamete generation; ovarian nurse cell to oocyte transport; organelle organization and biogenesis; mating; embryonic development via the syncytial blastoderm; cell communication; transport; cellular macromolecule metabolic process; embryonic development ending in birth or egg hatching; actin filament-based process. 41 alleles are reported. The phenotypes of these alleles are annotated with 26 unique terms, many of which group under: organ system; cephalopharyngeal skeleton; anatomical structure; embryonic head; adult; actin cytoskeleton; embryonic/larval tracheal system; pharyngeal sclerite; embryonic foregut; embryonic tagma. It has 6 annotated transcripts and 6 annotated polypeptides.
External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Src2 Src transcripts abundant in early embryos and in adult ovaries; also found in imaginal disks. Level declines in later embryos and is low in males and ovarectomized females. Inferred to be maternally acting gene whose product is required for early embryogenesis (Wadsworth et al.). Monoclonal antibodies detect two protein products of Src2, a doublet of 66 kd and one of 55 kd; the 55 kd polypeptide appears to initiate at the third methionine codon of the sequence; a faint 95 kd band appears to track the 66 kd band in developmental Northern blots. Monoclonal antibodies that differentiate between the two protein products show that they are differentially regulated. The 66 kd polypeptide first appears at the periphery of cells of the cellular blastoderm; this staining is transiently resolved into 13 or 14 segmental stripes plus uncharacterized staining in the head region in the fully extended germ band; no 66 kd protein detectable after 12 h of development. The 55 kd polypeptide first appears at 4-6 h and is widely distributed in the central and peripheral nervous systems. Larvae transformed with constructs that produce either the 66 or the 55 kd products under Hsp70 promoter action display cell-surface localization of the 66 kd protein and cytoplasmic localization of the 55 kd protein in third-instar salivary glands.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 29A1-29A3 Limits computationally determined from genome sequence between P{lacW}l(2)k16919^k16919&P{lacW}Bsg^k09030 and P{lacW}Btk29A^k00206&P{EP}Btk29A^EP715
Experimentally Determined Cytological Location
Cytogenetic map Notes References 29A-29A (Guarnieri, 1998.1.21) 29A1-29A2 (determined by in situ hybridisation) (Spradling et al., 1999) 29A-29A (determined by in situ hybridisation) (Baba et al., 1999) 29A1-29A2 (BDGP Project Members, 1994-1999) 29A-29A (determined by in situ hybridisation) (Katzen et al., 1990) 29A-29A (determined by in situ hybridisation) (Vincent et al., 1989) 29A-29A (determined by in situ hybridisation) (Gregory et al., 1987) 29A-29A (determined by in situ hybridisation) (Simon et al., 1983)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Btk29A for information on other features
Comments on Gene Model
	EST data support existence of multiple transcripts
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Btk29A-RA FBtr0079618 NM_057398 3273 603 Btk29A-RB FBtr0079616 NM_057397 3414 786 Btk29A-RC FBtr0079615 NM_164805 3276 603 Btk29A-RD FBtr0079617 NM_164804 3668 786 Btk29A-RE FBtr0112836 NM_001103651 3894 786 Btk29A-RF FBtr0112835 NM_001103650 5056 603
Additional Transcript Data & Comments
Reported size (kB)	3.5 (northern blot) (Gregory et al., 1987) 4.0, 3.0 (northern blot) (Baba et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Btk29A-PA FBpp0079238 66.8 603 8.80 NP_476746 AAF52631 Btk29A-PB FBpp0079236 87.4 786 9.39 NP_476745 AAF52632 Btk29A-PC FBpp0079235 66.8 603 8.80 NP_723370 AAN11162 Btk29A-PD FBpp0079237 87.4 786 9.39 NP_723369 AAN11161 Btk29A-PE FBpp0111749 87.4 786 9.39 NP_001097121 ABV53649 Btk29A-PF FBpp0111748 66.8 603 8.80 NP_001097120 ABV53648
Additional Polypeptide Data & Comments
Reported size (kD)	66, 55 (kD) (Theroux and Wadsworth, 1992) 786, 603 (aa) (Baba et al., 1999) 590 (aa); 66 (kD) (Gregory et al., 1987)
Comments	In addition to the 66 and 55 kD Btk29A proteins, a 95 kD protein is observed on Western blots. (Vincent et al., 1989) In addition to the SH2, SH3 and kinase domains it shares with the type 1 Btk29A protein, the type 2 Btk29A protein has the PH (Pleckstrin homology) and TH (Tec homology) domains that are characteristic of the Btk family of tyrosine kinases. (Baba et al., 1999) The 55kD gene product of Btk29A differs from the 66kD product by the absence of the 125 N-terminal amino acids. When expressed in Sf9 cells via a recombinant baculovirus system it does not exhibit detectable protein tyrosine-kinase activity. The p55 product lacks an N-terminal myristylation signal. (Theroux and Wadsworth, 1992) The 66kD protein appears as a doublet on Western blots. (Baba et al., 1999)
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0003502
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, core (IPR000719) SH2 motif (IPR000980) Tyrosine protein kinase (IPR001245) Src homology-3 (IPR001452) Tec/Btk (IPR001562) Pleckstrin-like (IPR001849) Tyrosine protein kinase, active site (IPR008266) Protein kinase-like (IPR011009) Pleckstrin homology-type (IPR011993) Protein kinase ATP binding, conserved site (IPR017441)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA246761 AA264318 AA390591 AA391653 AA438503 AA440825 AA441318 AA441618 AA538669 AA540300 AA541036 AA950333 AA951150 AA979284 AB009840 BAA24063 AB009841 BAA24064 AE003620 AAF52631 AE003620 AAF52632 AE003620 AAN11161 AE003620 AAN11162 AF044337 AAB99858 AQ034168 AQ073362 AW942403 AW942474 AY069457 AAL39602 AY128441 AAM75034 BG638638 BH011378 BH634787 BH634791 BI212739 BI216557 BI232426 BI232483 BI232848 BI235536 BI242949 BI356604 BI356781 BI357130 BI367061 BI367344 BI372071 BI374495 BI375302 BI375857 BI482492 BI485183 BI486321 BI486354 BI486776 BI486795 BI567289 BI580569 BI588973 BI641051 BP540222 BP540223 BP540614 BP540615 BT011183 CK662260 CO177688 CO179032 CO185413 CO188209 CO196286 CO262453 CO262506 CO269924 CO273754 CO275980 CO276498 CO279816 CO280727 CO281719 CO281796 CO282986 CO283040 CO288184 CO288400 CO289014 CO289117 CO289167 CO291263 CO291492 CO293930 CO295397 CO301658 CO312743 CO317252 CO319121 CO319716 CO325311 CO331579 CO338442 CZ470809 CZ470810 CZ474711 CZ475686 CZ478767 CZ486937 CZ486938 CZ487406 CZ488124 EC051809 EC052317 EC052899 EC053321 EC054305 EC055867 EC056540 EC058499 EC058731 EC058956 EC063049 EC065034 EC065687 EC070456 EC073341 EC074258 EC078071 EC079675 EC079693 EC081613 EC082638 EC083345 EC086817 EC089929 EC196190 EC196299 EC204559 EC205141 EC205164 EC205654 EC207120 EC213013 EC214406 EC217675 EC218988 EC219326 EC220354 EC220528 EC222467 EC228663 EC234246 EC240382 EC241729 EC242369 EC243898 EC245641 EC245642 EC246157 EC258223 EC262555 LD04781 LD31469 M16599 AAA28912 X02305 CAA26170

UniProtKB/Swiss-Prot	P08630
UniProtKB/TrEMBL

Maps to	DS08544 (Roulier et al., 1998)
Does NOT map to
Identified with	LD18251 LD16208 RE17878 (BDGP Project Members, 2000-) Dm4224 (Spradling et al., 1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References point mutation Btk29A[e482] 2L:8,260,970..8,260,970 na_change=G8253201A evidence=experimental reported_na_change=G1473A\|FBrf0102812 pr_change=W689@\|Btk29A-P2 reported_pr_change=W491@\|FBrf0102812 (Guarnieri et al., 1998)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 6889
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference prepupal stage-pupal stage pupal/adult digestive system \| restricted (Katzen et al., 1990) adult stage adult dorsal vessel (Katzen et al., 1990) larval stage \| third instar imaginal disc (Gregory et al., 1987) embryonic stage \| stage 8-11 procephalic segment (Katzen et al., 1990)