Tec29, Dsrc28C, Tec29A, fic, dsrc29A
protein-tyrosine kinase - necessary for maintaining the equilibrium between monomeric actin and filamentous actin during invagination of the salivary placodes - functions during oogenesis as a key downstream effector of Src64 during ring canal growth - targets β-catenin, which functions downstream of Wnt4 in escort cells to terminate Drosophila germ cell proliferation through up-regulation of expression
Please see the JBrowse view of Dmel\Btk29A for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.55
Gene model reviewed during 5.45
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.44
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Gene model reviewed during 5.56
4.0, 3.0 (northern blot)
3.5 (northern blot)
786, 603 (aa)
66, 55 (kD)
590 (aa); 66 (kD)
The 55kD gene product of Btk29A differs from
the 66kD product by the absence of the 125 N-terminal amino acids. When
expressed in Sf9 cells via a recombinant baculovirus system it does not
exhibit detectable protein tyrosine-kinase activity. The p55 product lacks
an N-terminal myristylation signal.
In addition to the 66 and 55 kD Btk29A proteins, a 95 kD protein is observed on Western blots.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Btk29A using the Feature Mapper tool.
Comment: maternally deposited
Comment: anlage in statu nascendi
Comment: anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as ventral nerve cord anlage
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as posterior spiracle specific anlage
Btk29A transcript is expressed in posterior spiracle cells, and their precursors, from embryonic stage 11 through the end of embryogenesis.
Type 1 and type 2 Btk29A transcripts are expressed in mutually exclusive patterns in the pupal and larval genital and nervous systems. Type 2 transcript is expressed in the male pupal genital disc along the edge of the male genital primordium facing the lumen, which is thought to give rise to main body of the male genitalia. In the larval and pupal CNS, type 2 transcript is detected in the mushroom body and antennal lobe, while type 1 transcript is detected in dispersed neurons and midline glial cells.
Type 1 and type 2 Btk29A transcripts are expressed in mutually exclusive patterns in the pupal and larval genital and nervous systems. Type 1 transcript expression in the male pupal genital disc is restricted to the anterior bulbus, which will give rise to the internal genitalia.In the larval and pupal CNS, type 2 transcript is detected in the mushroom body and antennal lobe, while type 1 transcript is detected in dispersed neurons and midline glial cells.
Comment: reference states 4-14 hr AEL
Comment: reference states >=4 hr AEL
Btk29A protein is expressed in posterior spiracle cells, and their precursors, from embryonic stage 11 through the end of embryogenesis.
Type 2 protein is the predominant form in the female germline.
Btk29A protein is localized strongly to the cellularization front, and less strongly to the apico-lateral membrane, during cellularization of the embryo.
Btk29A protein is detected in egg chambers. It is detected in ring canals starting at region 2b of the germarium through the remainder of oogenesis.
The 55 kD Btk29A protein is first detected at 4-6 hr of embryogenesis, and persists through the rest of embryogenesis. It is likely that the 55 kD protein is the nervous-system-specific cytoplasmic form.
A reticular pattern of staining around the periphery of ectodermal cells is observed in cellular blastoderm embryos, and persists through early germ band extension. A transient (1 hr) 13-14 striped pattern of expression is observed at full germ band extension. After the striped pattern fades, Btk29A protein is observed in the central and peripheral nervous systems, and is no longer peripheral, but cytoplasmic.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Btk29A in GBrowse 22-31
2-27.5
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
monoclonal antibody
monoclonal
Source for merge of: Btk29A CG18355
Btk29A is required non cell-autonomously for the invagination of the spiracular chamber during morphogenesis of the embryonic respiratory system.
Btk29A plays a role in the cytoskeletal dynamics that occur during cellularization of the blastoderm.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Btk29A exerts pleiotropic functions in different developmental contexts in different tissues through the generation of distinct forms of protein products by means of alternative splicing.
Btk29A is required for the development of the male genitalia and substrates required for adult survival.
Btk29A is essential for head involution during embryogenesis and ring canal development during oogenesis.
Phylogenetic analysis of the PTK family.
Both the p55 and p66 products lack a N-terminal myristylation signal. 66kD gene product expressed in Sf9 cells via a recombinant baculovirus has protein tyrosine-kinase activity, but it does not autophosphorylate nor does it use classic 'src' substrates. 55kD gene product expressed in the same system does not exhibit detectable protein tyrosine-kinase activity. A deletion derivative of the 66kD gene product lacking the SH3 and SH2 domains also failed to phosphorylate Sf9 cell proteins.
The mutant stocks studied which show neoplastic growth have structural rearrangements in the Btk29A gene.
Structural and biochemical evidence suggest the gene encodes a protein tyrosine kinase.