FB2020_04, released Aug 18, 2020

Gene: Dmel\Btk29A

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General Information
Symbol
Dmel\Btk29A
Species
D. melanogaster
Name
Btk family kinase at 29A
Annotation Symbol
CG8049
Feature Type
protein_coding_gene
FlyBase ID
FBgn0003502
Gene Model Status
Current
Stock Availability
26 publicly available
Enzyme Name (EC)
Non-specific protein-tyrosine kinase (2.7.10.2)
Gene Snapshot
Btk family kinase at 29A (Btk29A) encodes a non-receptor tyrosine kinase with diverse roles including cellularization, morphogenesis, patterning and germ cell proliferation. [Date last reviewed: 2019-03-07]
Other Summaries
Also Known As

Tec29, Dsrc28C, Tec29A, fic, dsrc29A

Key Links
Genomic Location
Cytogenetic map
29A1-29A3
Sequence location
2L:8,258,373..8,301,079 [-]
Recombination map

2-31

RefSeq locus
NT_033779 REGION:8258373..8301079
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (30 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
protein tyrosine kinase activity
inferred from direct assay
(Theroux and Wadsworth, 1992)
inferred from mutant phenotype
(Hamada-Kawaguchi et al., 2014)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
ATP binding
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
non-membrane spanning protein tyrosine kinase activity
inferred from biological aspect of ancestor with PANTHER:PTN001231474
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (22 terms)
Terms Based on Experimental Evidence (17 terms)
CV Term
Evidence
References
actin cytoskeleton organization
inferred from mutant phenotype
(Djagaeva et al., 2005)
cellularization
inferred from mutant phenotype
(Thomas and Wieschaus, 2004)
determination of adult lifespan
inferred from mutant phenotype
(assigned by UniProt )
(Baba et al., 1999)
DNA endoreduplication
inferred from mutant phenotype
(Chandrasekaran and Beckendorf, 2005)
female germline ring canal formation
inferred from mutant phenotype
(Hamada-Kawaguchi et al., 2015, Roulier et al., 1998)
inferred from direct assay
(assigned by UniProt )
(Dodson et al., 1998, Guarnieri et al., 1998)
head involution
inferred from mutant phenotype
(Roulier et al., 1998)
imaginal disc-derived male genitalia development
inferred from mutant phenotype
(assigned by UniProt )
(Baba et al., 1999)
inferred from mutant phenotype
(Hamada et al., 2005)
oocyte karyosome formation
inferred from mutant phenotype
(Djagaeva et al., 2005)
oogenesis
inferred from mutant phenotype
(Djagaeva et al., 2005)
open tracheal system development
inferred from mutant phenotype
(Matusek et al., 2006)
ovarian fusome organization
inferred from mutant phenotype
(Djagaeva et al., 2005)
ovarian nurse cell to oocyte transport
inferred from mutant phenotype
(Djagaeva et al., 2005)
peptidyl-tyrosine phosphorylation
inferred from direct assay
(Theroux and Wadsworth, 1992)
inferred from mutant phenotype
(Hamada-Kawaguchi et al., 2015)
regulation of actin filament polymerization
inferred from mutant phenotype
(Chandrasekaran and Beckendorf, 2005)
salivary gland morphogenesis
inferred from mutant phenotype
(Chandrasekaran and Beckendorf, 2005)
sensitization
inferred from mutant phenotype
(Asztalos et al., 2007)
spiracle morphogenesis, open tracheal system
inferred from mutant phenotype
(Tsikala et al., 2014)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
courtship behavior
non-traceable author statement
(Sokolowski, 2001)
dorsal closure
traceable author statement
(Harden, 2002)
intracellular signal transduction
inferred from electronic annotation with InterPro:IPR001562
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
JNK cascade
traceable author statement
(Harden, 2002)
peptidyl-tyrosine autophosphorylation
inferred from biological aspect of ancestor with PANTHER:PTN001231474
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (5 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
apical part of cell
inferred from direct assay
(Tsikala et al., 2014)
cell periphery
inferred from direct assay
(Tsikala et al., 2014)
cytoplasm
inferred from direct assay
(Wadsworth et al., 1990)
germline ring canal
inferred from direct assay
(assigned by UniProt )
(Dodson et al., 1998, Guarnieri et al., 1998)
plasma membrane
inferred from direct assay
(Wadsworth et al., 1990)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily. (P08630)
Protein Signatures (InterPro)
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate (2.7.10.2)
Summaries
Gene Group (FlyBase)
NON-RECEPTOR TYROSINE KINASES -
Non-Receptor Tyrosine kinases (nRTK) are cytoplasmic protein kinases that specifically phosphorylate tyrosine residues. nRTKs are involved in signalling cascades and possess targeting domains such as SH2 domains. (Adapted from FBrf0132098).
Protein Function (UniProtKB)
Required for proper ring canal development. Also required for the development of male genitalia and for adult survival.
(UniProt, P08630)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Src2
Src transcripts abundant in early embryos and in adult ovaries; also found in imaginal disks. Level declines in later embryos and is low in males and ovarectomized females. Inferred to be maternally acting gene whose product is required for early embryogenesis (Wadsworth et al.). Monoclonal antibodies detect two protein products of Src2, a doublet of 66 kd and one of 55 kd; the 55 kd polypeptide appears to initiate at the third methionine codon of the sequence; a faint 95 kd band appears to track the 66 kd band in developmental Northern blots. Monoclonal antibodies that differentiate between the two protein products show that they are differentially regulated. The 66 kd polypeptide first appears at the periphery of cells of the cellular blastoderm; this staining is transiently resolved into 13 or 14 segmental stripes plus uncharacterized staining in the head region in the fully extended germ band; no 66 kd protein detectable after 12 h of development. The 55 kd polypeptide first appears at 4-6 h and is widely distributed in the central and peripheral nervous systems. Larvae transformed with constructs that produce either the 66 or the 55 kd products under Hsp70 promoter action display cell-surface localization of the 66 kd protein and cytoplasmic localization of the 55 kd protein in third-instar salivary glands.
Summary (Interactive Fly)

protein-tyrosine kinase - necessary for maintaining the equilibrium between monomeric actin and filamentous actin during invagination of the salivary placodes - functions during oogenesis as a key downstream effector of Src64 during ring canal growth - targets β-catenin, which functions downstream of Wnt4 in escort cells to terminate Drosophila germ cell proliferation through up-regulation of expression

Gene Model and Products
Number of Transcripts
8
Number of Unique Polypeptides
4

Please see the JBrowse view of Dmel\Btk29A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.55

Gene model reviewed during 5.45

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.44

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Btk29A-RA
FBtr0079618
NM_057398
4026
603
Btk29A-RB
FBtr0079616
NM_057397
3420
786
Btk29A-RC
FBtr0079615
NM_164805
3279
603
Btk29A-RD
FBtr0079617
NM_164804
3674
786
Btk29A-RE
FBtr0112836
NM_001103651
4785
786
Btk29A-RF
FBtr0112835
NM_001103650
4992
603
Btk29A-RG
FBtr0330692
NM_001273314
3302
565
Btk29A-RH
FBtr0330693
NM_001273315
3826
464
Additional Transcript Data and Comments
Reported size (kB)

4.0, 3.0 (northern blot)

(Baba et al., 1999)

3.5 (northern blot)

(Gregory et al., 1987)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Btk29A-PA
FBpp0079238
66.8
603
8.80
NP_476746
AAF52631
Btk29A-PB
FBpp0079236
87.4
786
9.39
NP_476745
AAF52632
Btk29A-PC
FBpp0079235
66.8
603
8.80
NP_723370
AAN11162
Btk29A-PD
FBpp0079237
87.4
786
9.39
NP_723369
AAN11161
Btk29A-PE
FBpp0111749
87.4
786
9.39
NP_001097121
ABV53649
Btk29A-PF
FBpp0111748
66.8
603
8.80
NP_001097120
ABV53648
Btk29A-PG
FBpp0303535
62.4
565
8.74
NP_001260243
AGB92779
Btk29A-PH
FBpp0303536
52.9
464
8.60
NP_001260244
AGB92780
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

603 aa isoforms: Btk29A-PA, Btk29A-PC, Btk29A-PF
786 aa isoforms: Btk29A-PB, Btk29A-PD, Btk29A-PE
Additional Polypeptide Data and Comments
Reported size (kDa)

786, 603 (aa)

(Baba et al., 1999)

66, 55 (kD)

(Theroux and Wadsworth, 1992)

590 (aa); 66 (kD)

(Gregory et al., 1987)
Comments

The 55kD gene product of Btk29A differs from

the 66kD product by the absence of the 125 N-terminal amino acids. When

expressed in Sf9 cells via a recombinant baculovirus system it does not

exhibit detectable protein tyrosine-kinase activity. The p55 product lacks

an N-terminal myristylation signal.

(Theroux and Wadsworth, 1992)

In addition to the 66 and 55 kD Btk29A proteins, a 95 kD protein is observed on Western blots.

(Vincent et al., 1989)
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Btk29A using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
dot blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
(Wadsworth et al., 1985)
oogenesis & adult stage | female
ovary
(Wadsworth et al., 1985)
embryonic stage
(Wadsworth et al., 1985)
prepupal stage -- pupal stage
(Wadsworth et al., 1985)
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
epithelial cell

Comment: clustered apical distribution

(Lecuyer et al., 2007)
epithelium
(Lecuyer et al., 2007)
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
foregut anlage in statu nascendi
(Fisher et al., 2012)
organism | gap expression pattern
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
embryonic stage 7 -- 8
antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
anterior endoderm anlage
(Fisher et al., 2012)
dorsal ectoderm
(Fisher et al., 2012)
foregut anlage
(Fisher et al., 2012)
hindgut anlage
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
anterior endoderm
(Fisher et al., 2012)
dorsal ectoderm
(Fisher et al., 2012)
foregut primordium
(Fisher et al., 2012)
inclusive hindgut primordium
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
dorsal epidermis primordium
(Fisher et al., 2012)
foregut primordium
(Fisher et al., 2012)
hindgut proper primordium
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
posterior spiracle primordium

Comment: reported as posterior spiracle specific anlage

(Fisher et al., 2012)
salivary gland body primordium
(Fisher et al., 2012)
salivary gland duct primordium
(Fisher et al., 2012)
somatic muscle primordium
(Fisher et al., 2012)
tracheal primordium
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic dorsal epidermis
(Fisher et al., 2012)
embryonic foregut
(Fisher et al., 2012)
embryonic head epidermis
(Fisher et al., 2012)
embryonic hindgut
(Fisher et al., 2012)
embryonic/larval dorsal vessel
(Fisher et al., 2012)
embryonic/larval nervous system
(Fisher et al., 2012)
embryonic/larval posterior spiracle
(Fisher et al., 2012)
embryonic/larval somatic muscle
(Fisher et al., 2012)
lateral cord
(Fisher et al., 2012)
ventral nerve cord
(Fisher et al., 2012)
embryonic stage 11 -- 17
posterior spiracle primordium
(Tsikala et al., 2014)
third instar larval stage
embryonic/larval garland cell
(Tutor et al., 2014)
wandering third instar larval stage
wing pouch | restricted
(Molnar et al., 2012)
wing hinge primordium | restricted
(Molnar et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage -- pupal stage
midline glial cell
(Baba et al., 1999)
neuron | subset
(Baba et al., 1999)
mushroom body
(Baba et al., 1999)
antennal lobe
(Baba et al., 1999)
pupal stage
male genital disc | restricted
(Baba et al., 1999)
adult stage
adult head
(Baba et al., 1999)
(Baba et al., 1999)
adult stage & oogenesis
oocyte
(Gregory et al., 1987)
nurse cell
(Gregory et al., 1987)
adult stage | female
(Gregory et al., 1987)
embryonic stage
(Gregory et al., 1987)
third instar larval stage
imaginal disc
(Gregory et al., 1987)
radioisotope in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 9 -- 12
anterior midgut primordium
(Katzen et al., 1990)
proctodeum
(Katzen et al., 1990)
posterior midgut primordium
(Katzen et al., 1990)
embryonic stage 16 -- 17
epidermis
(Katzen et al., 1990)
embryonic stage 12 -- 14
embryonic/larval salivary gland
(Katzen et al., 1990)
embryonic stage 5 -- 6
(Katzen et al., 1990)
embryonic stage 1 -- 4
organism | ubiquitous
(Katzen et al., 1990)
embryonic stage 8 -- 11
procephalic segment
(Katzen et al., 1990)
ectoderm | segmentally repeated
(Katzen et al., 1990)
amnioserosa
(Katzen et al., 1990)
adult stage
adult proventriculus
(Katzen et al., 1990)
adult dorsal vessel
(Katzen et al., 1990)
ejaculatory bulb | restricted
(Katzen et al., 1990)
pupal stage
imaginal disc | restricted
(Katzen et al., 1990)
genital disc | restricted
(Katzen et al., 1990)
third instar larval stage
embryonic/larval lymph gland
(Katzen et al., 1990)
imaginal disc
(Katzen et al., 1990)
gonad
(Katzen et al., 1990)
adult stage & oogenesis
nurse cell
(Katzen et al., 1990)
ovary
(Katzen et al., 1990)
prepupal stage -- pupal stage
pupal/adult digestive system | restricted
(Katzen et al., 1990)
embryonic stage 15
trachea | restricted
(Katzen et al., 1990)
embryonic/larval posterior spiracle
(Katzen et al., 1990)
embryonic stage 12 -- 17
presumptive embryonic/larval central nervous system | presumptive
(Katzen et al., 1990)
prepupal stage
histoblast
(Katzen et al., 1990)
lamellocyte
(Katzen et al., 1990)
embryonic stage 12
gnathal segment
(Katzen et al., 1990)
stomodeal invagination | anterior
(Katzen et al., 1990)
labral segment
(Katzen et al., 1990)
larval stage -- adult stage
central nervous system
(Katzen et al., 1990)
embryonic stage 5
amnioserosa anlage in statu nascendi
(Katzen et al., 1990)
hindgut anlage | restricted

Comment: proctodeum anlage

(Katzen et al., 1990)
Additional Descriptive Data

Btk29A transcript is expressed in posterior spiracle cells, and their precursors, from embryonic stage 11 through the end of embryogenesis.

(Tsikala et al., 2014)

Type 1 and type 2 Btk29A transcripts are expressed in mutually exclusive patterns in the pupal and larval genital and nervous systems. Type 2 transcript is expressed in the male pupal genital disc along the edge of the male genital primordium facing the lumen, which is thought to give rise to main body of the male genitalia. In the larval and pupal CNS, type 2 transcript is detected in the mushroom body and antennal lobe, while type 1 transcript is detected in dispersed neurons and midline glial cells.

(Baba et al., 1999)

Type 1 and type 2 Btk29A transcripts are expressed in mutually exclusive patterns in the pupal and larval genital and nervous systems. Type 1 transcript expression in the male pupal genital disc is restricted to the anterior bulbus, which will give rise to the internal genitalia.In the larval and pupal CNS, type 2 transcript is detected in the mushroom body and antennal lobe, while type 1 transcript is detected in dispersed neurons and midline glial cells.

(Baba et al., 1999)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
epitope tag
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female
ring canal
(Lu et al., 2004)
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
extended germ band stage
organism | striped
(Vincent et al., 1989)
first instar larval stage
embryonic/larval central nervous system
(Vincent et al., 1989)
embryonic stage 13 -- 17
peripheral nervous system
(Vincent et al., 1989)
presumptive embryonic/larval central nervous system
(Vincent et al., 1989)
adult stage & oogenesis
ring canal
(Roulier et al., 1998)
embryonic stage 5
furrow canal | restricted
(Thomas and Wieschaus, 2004)
embryonic stage 5 -- 17
organism
(Vincent et al., 1989)
embryonic stage 11 -- 17
posterior spiracle primordium
(Tsikala et al., 2014)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 9 -- 16

Comment: reference states 4-14 hr AEL

(Vincent et al., 1989)
embryonic stage 9 -- 17

Comment: reference states >=4 hr AEL

(Vincent et al., 1989)
adult stage | female
ovary
(Lu et al., 2004)
Additional Descriptive Data

Btk29A protein is expressed in posterior spiracle cells, and their precursors, from embryonic stage 11 through the end of embryogenesis.

(Tsikala et al., 2014)

Type 2 protein is the predominant form in the female germline.

(Lu et al., 2004)

Btk29A protein is localized strongly to the cellularization front, and less strongly to the apico-lateral membrane, during cellularization of the embryo.

(Thomas and Wieschaus, 2004)

Btk29A protein is detected in egg chambers. It is detected in ring canals starting at region 2b of the germarium through the remainder of oogenesis.

(Roulier et al., 1998)

The 55 kD Btk29A protein is first detected at 4-6 hr of embryogenesis, and persists through the rest of embryogenesis. It is likely that the 55 kD protein is the nervous-system-specific cytoplasmic form.

(Vincent et al., 1989)

A reticular pattern of staining around the periphery of ectodermal cells is observed in cellular blastoderm embryos, and persists through early germ band extension. A transient (1 hr) 13-14 striped pattern of expression is observed at full germ band extension. After the striped pattern fades, Btk29A protein is observed in the central and peripheral nervous systems, and is no longer peripheral, but cytoplasmic.

(Vincent et al., 1989)
Marker for
 
ring canal
(Lu et al., 2004)
Subcellular Localization
CV Term
Evidence
References
apical part of cell
inferred from direct assay
(Tsikala et al., 2014)
cell periphery
inferred from direct assay
(Tsikala et al., 2014)
cytoplasm
inferred from direct assay
(Wadsworth et al., 1990)
germline ring canal
inferred from direct assay
(assigned by UniProt )
(Dodson et al., 1998, Guarnieri et al., 1998)
plasma membrane
inferred from direct assay
(Wadsworth et al., 1990)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Btk29A in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 39 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 35 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Btk29A
Transgenic constructs containing regulatory region of Btk29A
Deletions and Duplications ( 7 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal (with Df(2L)BSC200)
lethal | recessive
lethal - all die before end of embryonic stage | partially (with Btk29Ak00206)
lethal - all die before end of embryonic stage | partially (with Btk29Ak05610)
lethal - all die before end of embryonic stage | recessive
lethal - all die before end of first instar larval stage
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female semi-sterile, with Scer\GAL4nos.PG, Scer\GAL4VP16.nos.UTR, Scer\GAL4VP16.otu
female semi-sterile, with Scer\GAL4VP16.mat.αTub67C
male sterile
Other Phenotypes
Allele
behavior defective
cell shape defective
short lived
size defective, with Scer\GAL4en-e16E
some die during embryonic stage | partially (with Btk29Ak00206)
some die during embryonic stage | partially (with Btk29Ak05610)
some die during embryonic stage | recessive
some die during first instar larval stage
wild-type
Phenotype manifest in
Allele
actin cytoskeleton
actin filament | embryonic stage 5 | germline clone | maternal effect
actin filament | germline clone
actin filament bundle | embryonic stage 17
cephalopharyngeal skeleton
dorsal bridge
egg
egg chamber
egg chamber & actin cytoskeleton | germ-line clone
embryonic/first instar larval cuticle
embryonic/first instar larval cuticle (with Btk29Ak00206)
embryonic/first instar larval cuticle (with Btk29Ak05610)
embryonic/first instar larval cuticle (with Df(2L)TE29Aa-14)
embryonic/larval dorsal trunk | embryonic stage 17
embryonic/larval muscle system
embryonic/larval posterior spiracle
embryonic/larval salivary gland, with Scer\GAL4fkh.PH
embryonic/larval trachea
embryonic head
epipharyngeal sclerite
female germline cell
filzkorper
follicle cell
germarium, with Scer\GAL4C587
hypostomal sclerite
karyosome | germline clone
labrum
male genitalia
mouth hook | posterior
nurse cell fusome | germline clone
nurse cell ring canal
nurse cell ring canal | germline clone
oocyte | oogenesis stage S14
ovary
phallapodeme
phallapodeme | ectopic
phallapodeme | ectopic (with Btk29Afic-P)
phallapodeme | ectopic (with Btk29AL1-3)
posterior ejaculatory duct
presumptive embryonic salivary gland
presumptive embryonic salivary gland (with Btk29Ak00206)
presumptive embryonic salivary gland (with Btk29Ak05610)
salivary gland common duct primordium
spectrosome, with Scer\GAL4C587
spiracular chamber
stigmatophore
taenidial fold
taenidial fold | embryonic stage 17
vertical bridge | anterior
wing | posterior, with Scer\GAL4en-e16E
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (23)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\TEC
12 of 15
Yes
Yes
Hsap\TXK
10 of 15
No
Yes
Hsap\ITK
9 of 15
No
Yes
Hsap\BTK
8 of 15
No
Yes
 
3  
Hsap\BMX
7 of 15
No
Yes
Hsap\PTK6
3 of 15
No
Yes
Hsap\SRMS
3 of 15
No
Yes
Hsap\BLK
2 of 15
No
No
Hsap\CSK
2 of 15
No
No
Hsap\FRK
2 of 15
No
No
Hsap\FYN
2 of 15
No
No
2  
Hsap\HCK
2 of 15
No
No
Hsap\LCK
2 of 15
No
No
Hsap\LYN
2 of 15
No
No
Hsap\MATK
2 of 15
No
No
1  
Hsap\SRC
2 of 15
No
No
Hsap\YES1
2 of 15
No
No
Hsap\FGR
1 of 15
No
No
Hsap\JAK1
1 of 15
No
No
Hsap\JAK2
1 of 15
No
No
Hsap\JAK3
1 of 15
No
No
Hsap\TYK2
1 of 15
No
No
Hsap\ZBTB24
1 of 15
No
No
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (22)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Tec
12 of 15
Yes
Yes
Mmus\Txk
10 of 15
No
Yes
Mmus\Itk
9 of 15
No
Yes
Mmus\Btk
8 of 15
No
Yes
Mmus\Bmx
7 of 15
No
Yes
Mmus\Ptk6
3 of 15
No
Yes
Mmus\Srms
3 of 15
No
Yes
Mmus\Blk
2 of 15
No
No
Mmus\Csk
2 of 15
No
No
Mmus\Fgr
2 of 15
No
No
Mmus\Frk
2 of 15
No
No
Mmus\Fyn
2 of 15
No
No
Mmus\Hck
2 of 15
No
No
Mmus\Lck
2 of 15
No
No
Mmus\Lyn
2 of 15
No
No
Mmus\Matk
2 of 15
No
No
Mmus\Src
2 of 15
No
No
Mmus\Yes1
2 of 15
No
No
Mmus\Jak1
1 of 15
No
No
Mmus\Jak2
1 of 15
No
No
Mmus\Jak3
1 of 15
No
No
Mmus\Tyk2
1 of 15
No
No
Rattus norvegicus (Norway rat) (20)
Rnor\Itk
9 of 13
Yes
Yes
Rnor\Txk
8 of 13
No
Yes
Rnor\Tec
7 of 13
No
Yes
Rnor\Bmx
5 of 13
No
Yes
Rnor\Ptk6
3 of 13
No
Yes
Rnor\Srms
3 of 13
No
Yes
Rnor\Blk
2 of 13
No
No
Rnor\Btk
2 of 13
No
Yes
Rnor\Csk
2 of 13
No
No
Rnor\Fgr
2 of 13
No
No
Rnor\Frk
2 of 13
No
No
Rnor\Fyn
2 of 13
No
No
Rnor\Hck
2 of 13
No
No
Rnor\Lck
2 of 13
No
No
Rnor\Lyn
2 of 13
No
No
Rnor\Matk
2 of 13
No
No
Rnor\Src
2 of 13
No
No
Rnor\Yes1
2 of 13
No
No
Rnor\Jak1
1 of 13
No
No
Rnor\Tyk2
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (20)
Xtro\tec
6 of 12
Yes
Yes
Xtro\btk
2 of 12
No
Yes
Xtro\fgr
2 of 12
No
No
Xtro\fyn
2 of 12
No
No
Xtro\lck
2 of 12
No
No
Xtro\lyn
2 of 12
No
No
Xtro\src
2 of 12
No
No
Xtro\yes1
2 of 12
No
No
Xtro\blk
1 of 12
No
No
Xtro\csk
1 of 12
No
No
Xtro\frk
1 of 12
No
No
Xtro\hck
1 of 12
No
No
Xtro\jak1
1 of 12
No
No
Xtro\jak2
1 of 12
No
No
Xtro\jak3
1 of 12
No
No
Xtro\LOC100491469
1 of 12
No
No
Xtro\LOC101733045
1 of 12
No
Yes
Xtro\ptk6
1 of 12
No
No
Xtro\srms
1 of 12
No
No
Xtro\tyk2
1 of 12
No
No
Danio rerio (Zebrafish) (23)
Drer\btk
7 of 15
Yes
Yes
Drer\tec
7 of 15
Yes
Yes
Drer\itk
6 of 15
No
Yes
Drer\txk
6 of 15
No
Yes
Drer\ptk6b
3 of 15
No
Yes
Drer\csk
2 of 15
No
No
Drer\frk
2 of 15
No
No
Drer\fyna
2 of 15
No
No
Drer\fynb
2 of 15
No
No
Drer\hck
2 of 15
No
No
Drer\lck
2 of 15
No
No
Drer\lyn
2 of 15
No
No
Drer\ptk6a
2 of 15
No
Yes
Drer\si:ch73-340m8.2
2 of 15
No
No
Drer\yes1
2 of 15
No
No
Drer\zgc:194282
2 of 15
No
Yes
Drer\blk
1 of 15
No
No
Drer\jak1
1 of 15
No
No
Drer\jak2a
1 of 15
No
No
Drer\jak2b
1 of 15
No
No
Drer\matk
1 of 15
No
No
Drer\src
1 of 15
No
No
Drer\yrk
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (7)
Cele\src-1
2 of 15
Yes
No
Cele\src-2
2 of 15
Yes
No
Cele\Y47G6A.5
2 of 15
Yes
Yes
Cele\abl-1
1 of 15
No
No
Cele\kin-6
1 of 15
No
No
Cele\rol-3
1 of 15
No
No
Cele\T06C10.3
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (31)
Atha\AT1G17540
1 of 9
Yes
Yes
Atha\AT1G48220
1 of 9
Yes
Yes
Atha\AT1G55200
1 of 9
Yes
Yes
Atha\AT1G66460
1 of 9
Yes
Yes
Atha\AT1G70130
1 of 9
Yes
Yes
Atha\AT1G70450
1 of 9
Yes
Yes
Atha\AT2G19230
1 of 9
Yes
Yes
Atha\AT3G17410
1 of 9
Yes
Yes
Atha\AT3G58760
1 of 9
Yes
Yes
Atha\AT4G02630
1 of 9
Yes
Yes
Atha\AT4G04960
1 of 9
Yes
Yes
Atha\AT5G18500
1 of 9
Yes
Yes
Atha\AT5G18910
1 of 9
Yes
Yes
Atha\AT5G37790
1 of 9
Yes
Yes
Atha\AT5G65530
1 of 9
Yes
Yes
Atha\CRLK2
1 of 9
Yes
Yes
Atha\GPK1
1 of 9
Yes
No
Atha\PERK1
1 of 9
Yes
Yes
Atha\PERK3
1 of 9
Yes
Yes
Atha\PERK4
1 of 9
Yes
Yes
Atha\PERK5
1 of 9
Yes
Yes
Atha\PERK6
1 of 9
Yes
Yes
Atha\PERK7
1 of 9
Yes
Yes
Atha\PERK15
1 of 9
Yes
Yes
Atha\RBK1
1 of 9
Yes
Yes
Atha\RBK2
1 of 9
Yes
Yes
Atha\RKF1
1 of 9
Yes
Yes
Atha\SERK1
1 of 9
Yes
Yes
Atha\SRF8
1 of 9
Yes
Yes
Atha\SUB
1 of 9
Yes
Yes
Atha\VIK
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091903G3 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00000391
Drosophila simulans
Dsim\GD22424
Drosophila sechellia
Dsec\GM13100
Drosophila erecta
Dere\GG23459
Drosophila yakuba
Dyak\GE11074
Drosophila ananassae
Dana\GF15268
Drosophila pseudoobscura pseudoobscura
Dpse\GA20789
Drosophila persimilis
Dper\GL19386
Drosophila willistoni
Dwil\GK23856
Drosophila virilis
Dvir\GJ20581
Drosophila mojavensis
Dmoj\GI14244
Drosophila grimshawi
Dgri\GH10183
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500SQ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17021015
Musca domestica
House fly
Mdoa\17011701
Glossina morsitans
Tsetse fly
Gmor\GMOY011025
Glossina morsitans
Tsetse fly
Gmor\GMOY004919
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_03923
Mayetiola destructor
Hessian fly
Mdes\Mdes007557
Mayetiola destructor
Hessian fly
Mdes\Mdes009665
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL003937
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL012752
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL008777
Anopheles darlingi
American malaria mosquito
Adar\ADAC010197
Anopheles darlingi
American malaria mosquito
Adar\ADAC008874
Anopheles gambiae
Malaria mosquito
Agam\AGAP004989
Anopheles gambiae
Malaria mosquito
Agam\AGAP008317
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ009602
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ000833
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ003985
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00MF )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA000973
Bombyx mori
Silkmoth
Bmor\BGIBMGA004089
Bombyx mori
Silkmoth
Bmor\BGIBMGA002069
Bombyx mori
Silkmoth
Bmor\BGIBMGA012094
Bombyx mori
Silkmoth
Bmor\BGIBMGA000974
Bombyx mori
Silkmoth
Bmor\BGIBMGA000972
Danaus plexippus
Monarch butterfly
Dple\DPOGS207312-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS207465-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS200085-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS211780-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS211781-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL016683
Heliconius melpomene
Postman butterfly
Hmel\HMEL015762
Heliconius melpomene
Postman butterfly
Hmel\HMEL015066
Heliconius melpomene
Postman butterfly
Hmel\HMEL017653
Apis florea
Little honeybee
Aflo\2188804676
Apis florea
Little honeybee
Aflo\2802943476
Apis florea
Little honeybee
Aflo\2623606004
Apis florea
Little honeybee
Aflo\2152282052
Apis mellifera
Western honey bee
Amel\LOC412588
Apis mellifera
Western honey bee
Amel\GB47566
Apis mellifera
Western honey bee
Amel\LOC409127
Apis mellifera
Western honey bee
Amel\GB46371
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP17868
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP21745
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP16169
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP12987
Bombus terrestris
Buff-tailed bumblebee
Bter\15208566
Bombus terrestris
Buff-tailed bumblebee
Bter\15209628
Bombus terrestris
Buff-tailed bumblebee
Bter\15213667
Bombus terrestris
Buff-tailed bumblebee
Bter\15205435
Linepithema humile
Argentine ant
Lhum\LH10586
Linepithema humile
Argentine ant
Lhum\LH11572
Linepithema humile
Argentine ant
Lhum\LH24553
Linepithema humile
Argentine ant
Lhum\LH22348
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0015bc
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0002f3
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001ea3
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG009131
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG016443
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG006407
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG009605
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_00955
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_12391
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_08800
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_09867
Tribolium castaneum
Red flour beetle
Tcas\TC034634
Tribolium castaneum
Red flour beetle
Tcas\TC002522
Tribolium castaneum
Red flour beetle
Tcas\TC031200
Tribolium castaneum
Red flour beetle
Tcas\GLEAN_07809
Pediculus humanus
Human body louse
Phum\PHUM538010
Pediculus humanus
Human body louse
Phum\PHUM305940
Pediculus humanus
Human body louse
Phum\PHUM378190
Pediculus humanus
Human body louse
Phum\PHUM118390
Rhodnius prolixus
Kissing bug
Rpro\RPRC012995
Rhodnius prolixus
Kissing bug
Rpro\Src64B
Rhodnius prolixus
Kissing bug
Rpro\RPRC008334
Rhodnius prolixus
Kissing bug
Rpro\Src42
Cimex lectularius
Bed bug
Clec\CLEC005989
Cimex lectularius
Bed bug
Clec\CLEC010008
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI007038
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI001933
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI009437
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI001730
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_09483
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_03797
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_07944
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_05378
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01J8 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR013578
Ixodes scapularis
Black-legged tick
Isca\ISCW012174
Stegodyphus mimosarum
African social velvet spider
Smim\KFM59334.1
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_310538
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0D46 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Sfk3
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Sfk5
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Btk
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Frk
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Abl
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Sfk1h
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Skf6
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Frk_1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Sfk7
Ciona intestinalis
Vase tunicate
Cint\LOC445711
Ciona intestinalis
Vase tunicate
Cint\LOC100183015
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000016554
Ciona intestinalis
Vase tunicate
Cint\LOC100184615
Ciona intestinalis
Vase tunicate
Cint\LOC100180374
Ciona intestinalis
Vase tunicate
Cint\LOC100184799
Ciona intestinalis
Vase tunicate
Cint\LOC100177921
Gallus gallus
Domestic chicken
Ggal\SRC
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000004785
Gallus gallus
Domestic chicken
Ggal\SRMS
Gallus gallus
Domestic chicken
Ggal\LYN
Gallus gallus
Domestic chicken
Ggal\FRK
Gallus gallus
Domestic chicken
Ggal\BLK
Gallus gallus
Domestic chicken
Ggal\ITK
Gallus gallus
Domestic chicken
Ggal\BMX
Gallus gallus
Domestic chicken
Ggal\BTK
Gallus gallus
Domestic chicken
Ggal\FYN
Gallus gallus
Domestic chicken
Ggal\TEC
Gallus gallus
Domestic chicken
Ggal\YES1
Gallus gallus
Domestic chicken
Ggal\TXK
Gallus gallus
Domestic chicken
Ggal\ABL1
Gallus gallus
Domestic chicken
Ggal\LCK
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000004213
Gallus gallus
Domestic chicken
Ggal\HCK
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (13)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Csk
4 of 10
Src42A
4 of 10
Src64B
4 of 10
Ack-like
3 of 10
Shark
3 of 10
Abl
2 of 10
Ack
2 of 10
FER
2 of 10
CG10702
1 of 10
Fak
1 of 10
hop
1 of 10
Tie
1 of 10
Wsck
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 3 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    TEC; tec protein tyrosine kinase
    12 of 15
    600583
        TXK; TXK tyrosine kinase
        10 of 15
        600058
            ITK; IL2 inducible T cell kinase
            9 of 15
            186973
            BTK; Bruton tyrosine kinase
            8 of 15
            300300
             
            BMX; BMX non-receptor tyrosine kinase
            7 of 15
            300101
                PTK6; protein tyrosine kinase 6
                3 of 15
                602004
                    SRMS; src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation sites
                    3 of 15
                    617797
                        Functional Complementation Data
                        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                        Dmel gene
                        Ortholog showing functional complementation
                        Supporting References
                        Btk29A
                        Hsap\BTK
                        (Hamada et al., 2005)
                        Interactions
                        Summary of Physical Interactions
                        esyN Network Diagram
                        Show neighbor-neighbor interactions:
                        Select Layout:
                        Legend:
                        Protein
                        RNA
                        Selected Interactor(s)
                        Interactions Browser
                        View in Interactions Browser
                        Please see the Physical Interaction reports below for full details
                        protein-protein
                        Physical Interaction
                        Assay
                        References
                        Btk29A - Cad96Ca
                        two hybrid
                        (Tsarouhas et al., 2014)
                        Btk29A - Egfr
                        two hybrid
                        (Bianco et al., 2007)
                        Btk29A - Ptp10D
                        anti bait coimmunoprecipitation, western blot
                        (Tsarouhas et al., 2019)
                        Btk29A - Pvr
                        two hybrid
                        (Bianco et al., 2007)
                        Btk29A - wash
                        anti tag coimmunoprecipitation, western blot, anti tag western blot
                        (Tsarouhas et al., 2019)
                        Summary of Genetic Interactions
                        External Data
                        Linkouts
                        BioGRID - A database of protein and genetic interactions.
                        DroID - A comprehensive database of gene and protein interactions.
                        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                        MIST (genetic) - An integrated Molecular Interaction Database
                        MIST (protein-protein) - An integrated Molecular Interaction Database
                        Pathways
                        Signaling Pathways (FlyBase)
                        Metabolic Pathways
                        External Data
                        Genomic Location and Detailed Mapping Data
                        Chromosome (arm)
                        2L
                        Recombination map

                        2-31

                        Cytogenetic map
                        29A1-29A3
                        Sequence location
                        2L:8,258,373..8,301,079 [-]
                        FlyBase Computed Cytological Location
                        Cytogenetic map
                        Evidence for location
                        29A1-29A3
                        Limits computationally determined from genome sequence between P{lacW}l(2)k16919k16919&P{lacW}Bsgk09030 and P{lacW}Btk29Ak00206&P{EP}Btk29AEP715
                        Experimentally Determined Cytological Location
                        Cytogenetic map
                        Notes
                        References
                        29A-29A
                        (Guarnieri, 1998.1.21)
                        29A1-29A2
                        (determined by in situ hybridisation)
                        (Spradling et al., 1999)
                        29A-29A
                        (determined by in situ hybridisation)
                        (Baba et al., 1999, Katzen et al., 1990, Vincent et al., 1989, Gregory et al., 1987, Simon et al., 1983)
                        29A1-29A2
                        (BDGP Project Members, 1994-1999)
                        Experimentally Determined Recombination Data
                        Location

                        2-31

                        (FlyBase, 2016)

                        2-27.5

                        (Comeron et al., 2012)
                        Left of (cM)
                        Right of (cM)
                        Notes
                        Stocks and Reagents
                        Stocks (26)
                        Genomic Clones (43)
                        cDNA Clones (129)
                         

                        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

                        cDNA clones, fully sequenced
                        BDGP DGC clones
                        Other clones
                        Drosophila Genomics Resource Center cDNA clones

                        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                        cDNA Clones, End Sequenced (ESTs)
                        BDGP DGC clones
                        Other clones
                        RNAi and Array Information
                        Linkouts
                        DRSC - Results frm RNAi screens
                        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                        Antibody Information
                        Laboratory Generated Antibodies
                         

                        polyclonal

                        (Hamada-Kawaguchi et al., 2014, Tsikala et al., 2014)

                        monoclonal antibody

                        (Thomas and Wieschaus, 2004)

                        monoclonal

                        (Vincent et al., 1989)
                        Commercially Available Antibodies
                         
                        Other Information
                        Relationship to Other Genes
                        Source for database identify of
                        Source for database merge of

                        Source for merge of: Btk29A CG18355

                        (Manning, 2001.10.16)
                        Additional comments
                        Other Comments

                        Btk29A is required non cell-autonomously for the invagination of the spiracular chamber during morphogenesis of the embryonic respiratory system.

                        (Tsikala et al., 2014)

                        Btk29A plays a role in the cytoskeletal dynamics that occur during cellularization of the blastoderm.

                        (Thomas and Wieschaus, 2004)

                        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

                        (Kiger et al., 2003)

                        Btk29A exerts pleiotropic functions in different developmental contexts in different tissues through the generation of distinct forms of protein products by means of alternative splicing.

                        (Baba et al., 1999)

                        Btk29A is required for the development of the male genitalia and substrates required for adult survival.

                        (Baba et al., 1999)

                        Btk29A is essential for head involution during embryogenesis and ring canal development during oogenesis.

                        (Roulier et al., 1998)

                        Phylogenetic analysis of the PTK family.

                        (Suga et al., 1997)

                        Both the p55 and p66 products lack a N-terminal myristylation signal. 66kD gene product expressed in Sf9 cells via a recombinant baculovirus has protein tyrosine-kinase activity, but it does not autophosphorylate nor does it use classic 'src' substrates. 55kD gene product expressed in the same system does not exhibit detectable protein tyrosine-kinase activity. A deletion derivative of the 66kD gene product lacking the SH3 and SH2 domains also failed to phosphorylate Sf9 cell proteins.

                        (Theroux and Wadsworth, 1992)

                        The mutant stocks studied which show neoplastic growth have structural rearrangements in the Btk29A gene.

                        (Nabirochkin et al., 1991)

                        Examination of the expression of Btk29A two major proteins, p66 and p55, suggests that Btk29A plays a role in the development of numerous embryonic and larval tissues.

                        (Wadsworth et al., 1990)

                        Structural and biochemical evidence suggest the gene encodes a protein tyrosine kinase.

                        (Wadsworth, 1990)
                        Origin and Etymology
                        Discoverer
                        Etymology
                        Identification
                        External Crossreferences and Linkouts ( 107 )
                        Sequence Crossreferences
                        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                        Other crossreferences
                        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                        Flygut - An atlas of the Drosophila adult midgut
                        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                        InterPro - A database of protein families, domains and functional sites
                        KEGG Genes - Molecular building blocks of life in the genomic space.
                        modMine - A data warehouse for the modENCODE project
                        SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                        Linkouts
                        BioGRID - A database of protein and genetic interactions.
                        DPiM - Drosophila Protein interaction map
                        DroID - A comprehensive database of gene and protein interactions.
                        DRSC - Results frm RNAi screens
                        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
                        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                        FlyMine - An integrated database for Drosophila genomics
                        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
                        MIST (genetic) - An integrated Molecular Interaction Database
                        MIST (protein-protein) - An integrated Molecular Interaction Database
                        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                        Synonyms and Secondary IDs (39)
                        Reported As
                        Symbol Synonym
                        Btk
                        (Fernández et al., 2014)
                        Btk29
                        (Eikenes et al., 2015)
                        Btk29A
                        (Meltzer et al., 2019, Tsarouhas et al., 2019, Lee et al., 2018, Hamada-Kawaguchi and Yamamoto, 2017, Neuert et al., 2017, Tokusumi et al., 2017, Transgenic RNAi Project members, 2017-, Clandinin and Owens, 2016-, Gene Disruption Project members, 2016-, Hoi et al., 2016, Öztürk-Çolak et al., 2016, Öztürk-Çolak et al., 2016, Sunouchi et al., 2016, Gene Disruption Project members, 2015-, Hamada-Kawaguchi et al., 2015, Kavi et al., 2015, Ashwal-Fluss et al., 2014, Baril et al., 2014, Chen et al., 2014, Faisal et al., 2014, Hamada-Kawaguchi et al., 2014, Sopko et al., 2014, Tsikala et al., 2014, Tutor et al., 2014, Carter, 2013, de Celis et al., 2013.9.11, Djiane et al., 2013, Jagut et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Nawaz et al., 2012, Carreira et al., 2011, Juarez et al., 2011, Hazelett et al., 2009, Southall and Brand, 2009, Akdemir et al., 2007, Asztalos et al., 2007, Bianco et al., 2007, Lecuyer et al., 2007, Minidorff et al., 2007, Minidorff et al., 2007, Quinones-Coello, 2007, Quinones-Coello, 2007, Beckett and Baylies, 2006, Christensen and Cook, 2006.8.30, Christensen and Cook, 2006.12.5, Christensen and Cook, 2006.12.5, Christensen and Cook, 2006.12.5, Edwards et al., 2006, Molnar et al., 2006)
                        Btk29a
                        (Sytnikova et al., 2014)
                        C-src2
                        C-src4
                        CG18355
                        CG8049
                        (Neuert et al., 2017, Sienski et al., 2012, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Perkins et al., 2009.1.15, Quinones-Coello, 2007, Quinones-Coello, 2007, Molnar et al., 2006, Metaxakis et al., 2005)
                        CT2415
                        (Morrison et al., 2000)
                        CT41718
                        (Morrison et al., 2000)
                        DSrc28
                        (Palmer et al., 1999)
                        DTec29
                        (Palmer et al., 1999)
                        Dm SRC2
                        (Gibson and Spring, 1998)
                        Dsrc28C
                        (Dobrovolskaia and Belitsky, 1997, Suga et al., 1997, Yamamoto, 1997, Yamamoto et al., 1997, Woods and Bryant, 1993, Theroux and Wadsworth, 1992, Shishido et al., 1991, Wadsworth et al., 1990)
                        Dsrc29A
                        (Yamamoto, 1997.12.15, Yamamoto, 1997.12.15, Hoffmann, 1991)
                        EC2-9
                        (Baril et al., 2014)
                        S13
                        SRC 29A
                        (Morrison et al., 2000)
                        Src2
                        Src29A
                        (Roulier and Beckendorf, 1998)
                        Tec
                        (Lu et al., 2004, Roche, 1998)
                        Tec29
                        (Chougule et al., 2020, Öztürk-Çolak et al., 2016, Ríos-Barrera and Riesgo-Escovar, 2013, Matusek et al., 2006, Chandrasekaran and Beckendorf, 2005, Chandrasekaran and Beckendorf, 2005, Djagaeva, 2005, Djagaeva et al., 2005, Laberge et al., 2005, Lu et al., 2004, Lu et al., 2003, Stern et al., 2003, Hudson and Cooley, 2002, Jacinto et al., 2002, Muda et al., 2002, Stern and Beckendorf, 2002, Deng and Lin, 2001, Lu and Simon, 2001, Stern and Beckendorf, 2001, van der Flier and Sonnenberg, 2001, Roche, 2000, Stern et al., 2000, Tateno et al., 2000, Wilson et al., 2000, Cooley, 1998, Guarnieri, 1998.1.21, Guarnieri et al., 1998, Roulier et al., 1998, Simon et al., 1998)
                        Tec29A
                        (Csiszar et al., 2010, Matusek et al., 2005, Chandrasekaran and Beckendorf, 2004, Schnorr et al., 2001, Li et al., 2000, Jackson and Berg, 1999, Roulier and Beckendorf, 1998)
                        btk
                        (Roussou et al., 2019, Langton et al., 2007)
                        btk29
                        (Maybeck and Röper, 2009)
                        btk29A
                        (Levinson and Cagan, 2016, Tsarouhas et al., 2014, Langton et al., 2007)
                        btk29a
                        (McPhee et al., 2010)
                        c-src/fps
                        (Nabirochkin et al., 1991)
                        dsrc29A
                        (Manning and Krasnow, 1993, Katzen, 1990, Katzen et al., 1990)
                        fic
                        (Billeter et al., 2002, Baba et al., 1999, Yamamoto and Nakano, 1998, Yamamoto, 1997, Yamamoto et al., 1997)
                        src-4
                        (Pribyl et al., 1988)
                        src2
                        src28C
                        (Wadsworth, 1990)
                        src4
                        tec29
                        (Maybeck and Röper, 2009, Thomas and Wieschaus, 2004, Kelso et al., 2001, Kockel et al., 2001)
                        Name Synonyms
                        Btk family kinase at 29A
                        (Faisal et al., 2014, Edwards et al., 2006)
                        Btk29A type 2
                        (Hamada-Kawaguchi et al., 2014)
                        Src oncogene 29A
                        fickle
                        (Baba et al., 1997)
                        Secondary FlyBase IDs
                        • FBgn0032011
                        Datasets (0)
                        Study focus (0)
                        Experimental Role
                        Project
                        Project Type
                        Title
                        References (214)