FlyBase Gene Report: Dmel\stau

General Information

Symbol

Dmel\stau

Species

D. melanogaster

Name

staufen

Annotation Symbol

CG5753

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003520

Gene Model Status

Stock Availability

19 publicly available

Gene Snapshot

staufen (stau) encodes a double-stranded RNA binding protein involved in mRNA localization. It contributes to anterior and posterior patterning, nervous system development and long term memory. [Date last reviewed: 2019-03-14]

Other Summaries

Alliance
Auto summary
UniProtKB
Red Book
Interactive Fly

Also Known As

dStau

Key Links

Genomic Location

Cytogenetic map

Sequence location

2R:18,117,896..18,125,779 [-]

Recombination map

2-84

RefSeq locus

NT_033778 REGION:18117896..18125779

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (34 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

enables mRNA 3'-UTR binding

inferred from direct assay

(Jenny et al., 2006, Li et al., 1997)

enables mRNA binding

inferred from direct assay

(Mahoney et al., 2016)

enables protein binding

inferred from physical interaction with FLYBASE:mira; FB:FBgn0021776

(Jia et al., 2015)

enables RNA binding

inferred from direct assay

(Landskron et al., 2018)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables double-stranded RNA binding

inferred from sequence or structural similarity

(Lasko, 2000)

traceable author statement

(van Eeden and St. Johnston, 1999)

Biological Process (16 terms)

Terms Based on Experimental Evidence (10 terms)

CV Term

Evidence

References

involved_in anterior/posterior axis specification, embryo

inferred from genetic interaction with FLYBASE:mira; FB:FBgn0021776

(Irion et al., 2006)

involved_in intracellular mRNA localization

inferred from mutant phenotype

(Landskron et al., 2018)

involved_in pole plasm assembly

inferred from mutant phenotype

(Raff et al., 1990)

involved_in pole plasm protein localization

inferred from mutant phenotype

(Irion et al., 2006)

involved_in pole plasm RNA localization

inferred from mutant phenotype

(Raff et al., 1990)

involved_in positive regulation of cytoplasmic mRNA processing body assembly

inferred from mutant phenotype

(Eulalio et al., 2007)

involved_in positive regulation of synaptic growth at neuromuscular junction

inferred from mutant phenotype

(Gardiol and St Johnston, 2014)

involved_in protein localization to synapse

inferred from mutant phenotype

(Gardiol and St Johnston, 2014)

involved_in regulation of pole plasm oskar mRNA localization

inferred from genetic interaction with FLYBASE:mira; FB:FBgn0021776

(Irion et al., 2006)

involved_in RNA localization

inferred from mutant phenotype

(Li et al., 1997)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

involved_in asymmetric protein localization involved in cell fate determination

traceable author statement

(Bardin et al., 2004)

involved_in bicoid mRNA localization

traceable author statement

(Johnstone and Lasko, 2001, Ding et al., 1993)

involved_in long-term memory

traceable author statement

(Greenspan, 2003)

involved_in neuroblast fate determination

traceable author statement

(Bardin et al., 2004)

involved_in oogenesis

traceable author statement

(Wylie, 1999)

involved_in regulation of oskar mRNA translation

traceable author statement

(Mahowald, 2001, van Eeden and St. Johnston, 1999)

Cellular Component (13 terms)

Terms Based on Experimental Evidence (13 terms)

CV Term

Evidence

References

located_in apical cortex

inferred from direct assay

(Li et al., 1997)

located_in apical part of cell

inferred from direct assay

(Fuerstenberg et al., 1998)

located_in apical plasma membrane

inferred from direct assay

(Shen et al., 1998)

located_in basal cortex

inferred from direct assay

(Landskron et al., 2018, Fuerstenberg et al., 1998)

located_in basal plasma membrane

inferred from direct assay

(Shen et al., 1998)

located_in cell cortex

inferred from direct assay

(Li et al., 1997)

located_in cytoplasm

inferred from direct assay

(Fuerstenberg et al., 1998)

located_in germ plasm

inferred from direct assay

(assigned by BHF-UCL )

(Li et al., 2009)

part_of messenger ribonucleoprotein complex

inferred from direct assay

(assigned by UniProt )

(Barbee et al., 2006)

located_in neuronal cell body

inferred from direct assay

(assigned by UniProt )

(Hillebrand et al., 2010, Barbee et al., 2006)

located_in neuronal ribonucleoprotein granule

inferred from direct assay

(assigned by UniProt )

(Hillebrand et al., 2010, Barbee et al., 2006)

located_in P-body

inferred from direct assay

(Eulalio et al., 2007)

located_in subsynaptic reticulum

inferred from direct assay

(Gardiol and St Johnston, 2014)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

RNA-binding protein which forms ribonucleoprotein complexes (RNPs) that play critical roles in the localization, translational repression and turnover of RNAs during embryogenesis, neurotransmission and neurogenesis (PubMed:10698941, PubMed:1712672, PubMed:8001156, PubMed:17178403). In the oocyte, essential for the localization of both the osk/oskar mRNA to the posterior pole and bcd/bicoid RNA to the anterior pole, and is therefore required for the correct anterior-posterior patterning of the developing embryo (PubMed:10698941, PubMed:1712672, PubMed:8001156). Association with osk or bcd at their respective poles, appears to promote the formation and stabilization of the ribonucleoprotein complexes (PubMed:1712672, PubMed:8001156). Integral component of diverse neuritic ribonucleoprotein complexes (RNPs) that mediate the transport, translation and turnover of neuronal RNAs during neuorgenesis and the translation repression of synaptic transcripts in preparation for their dendritic targeting (PubMed:17178403).

(UniProt, P25159)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

stau: staufen (T. Schupbach)

Maternal-effect lethal. Embryos from homozygous mothers exhibit a so-called "grandchildless-knirps" phenotype; all eggs lack polar granules and no pole cells are formed; most embryos show variable deletions of abdominal segments, whereby segment A4 is deleted most frequently; larger deletions may delete segments A2 through A7; in extreme cases, anterior parts of segment A1 become fused to posterior parts of segment A8, but telson elements are always present and relatively normal. In addition, embryos show deletions of the anterior-most head structures and the cephalic furrow is shifted anteriorly at gastrulation. Analysis of germline clones indicates that the mutation is germline autonomous (Schupbach and Wieschaus, 1986, Dev. Biol. 113: 443-48).

Summary (Interactive Fly)

Double stranded RNA binding protein - required for the localization of multiple mRNA species during oogenesis and zygotic development -direct binding of Staufen to and mRNAs is a requirement for their subcellular distribution - as a posterior group protein Staufen required for the localization of mRNA to the posterior pole of the oocyte

Interactive Fly

Gene Model and Products

Number of Transcripts

3

Number of Unique Polypeptides

3

Please see the JBrowse view of Dmel\stau for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0086783

5240

1026

FBtr0086784

4910

914

FBtr0301614

3509

1025

Additional Transcript Data and Comments

Reported size (kB)

5.3 (northern blot)

(St. Johnston et al., 1991)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0085962

110.3

1026

9.63

FBpp0085963

98.0

914

9.51

FBpp0290829

110.2

1025

9.63

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

1026 (aa); 150 (kD observed)

(St. Johnston et al., 1991)

Comments

External Data

Subunit Structure (UniProtKB)

Component of neuronal ribonucleoprotein complexes (RNPs) that contains at least various translational repressor and mRNA turnover proteins such as me31B, tral, Upf1, AGO2 and sometimes Fmr1.

(UniProt, P25159)

Domain

Contains a proline-rich domain. The insertion of this domain in the DRBM 2 domain is required for stau-oskar mRNA localization.

DRBM 3 domain binds optimally to stem-loops containing 12 bp (in vitro).

(UniProt, P25159)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

1EKZ
1STU

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\stau using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage oogenesis stage S3 -- S4

female germline stem cell

(St. Johnston et al., 1991)

fertilized egg stage

fertilized egg | ubiquitous

(St. Johnston et al., 1991)

adult stage oogenesis stage S6 -- S10

(St. Johnston et al., 1991)

adult stage & oogenesis stage S6

oocyte | presumptive

(St. Johnston et al., 1991)

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

dorsal epidermis primordium

(Fisher et al., 2012)

dorsal sensory complex primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

embryonic stage 1 -- 4

(Kang et al., 2009)

embryonic stage 14

ventral nerve cord

(Kang et al., 2009)

Additional Descriptive Data

stau is maternally expressed in the embryo and later expression is observed in a symmetric pattern along the ventral nerve cord.

(Kang et al., 2009)

stau transcript is first detected in germline cells at stage S3-S4 of oogenesis. By stage S6, stau expression is concentrated in the presumptive oocyte, but is also detected in the nurse cells. By stage S10, stau transcript is predominantly in the nurse cells, which later transfer their cytoplasm to the oocyte. Thus, stau transcript is uniformly distributed in the freshly laid egg. At cellularization, stau transcript is found in the basal regions of the blastoderm cells, and persists until after gastrulation.

(St. Johnston et al., 1991)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis stage S10

oocyte | posterior

(Breitwieser et al., 1996)

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis stage S11

oocyte | anterior

(Martin et al., 2003)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

extended germ band stage

presumptive embryonic/larval nervous system

(St. Johnston et al., 1991)

embryonic stage 1

organism | 90-100% egg length

Comment: 95-100% egg length

(Ferrandon et al., 1994)

organism | 0-10% egg length

Comment: 0-5% egg length

(Ferrandon et al., 1994)

organism | terminal expression pattern

(Ferrandon et al., 1994)

adult stage oogenesis stage S10B -- S14

(St. Johnston et al., 1991)

embryonic stage 13

central nervous system

(St. Johnston et al., 1991)

oogenesis stage S3 -- S4

oocyte | presumptive

(St. Johnston et al., 1991)

adult stage oogenesis stage S8 -- S10B

oocyte | posterior

(St. Johnston et al., 1991)

oogenesis stage S6 -- S8

polar follicle cell | posterior

(Xi et al., 2003)

embryonic stage

embryonic neuroblast

• basal cortex

Comment: localized in a dense basal cresent as cells enter mitosis

(Fuerstenberg et al., 1998)

embryonic neuroblast

• apical cortex

Comment: localized in a diffuse apical cresent in mitotic cells

(Fuerstenberg et al., 1998)

embryonic ganglion mother cell

• cytoplasm

Comment: post-mitotic localization

(Fuerstenberg et al., 1998)

embryonic stage 4

primordial germ cell

(St. Johnston et al., 1991)

organism | anterior

(St. Johnston et al., 1991)

embryonic stage 1 -- 5

organism | posterior

(Breitwieser et al., 1996)

third instar larval stage

embryonic/larval neuromuscular junction

(Gardiol and St Johnston, 2014)

Comment: postsynaptic side

(Gardiol and St Johnston, 2014)

oogenesis stage S9 & adult stage | female

oocyte | posterior | restricted

(Ng et al., 2016)

oogenesis stage S10 & adult stage | female

oocyte | posterior | restricted

(Sanghavi et al., 2016)

oogenesis & adult stage | female

oocyte | posterior

(Vachias et al., 2014)

Additional Descriptive Data

stau protein localizes to the apical cortex of mitotic neuroblasts in late interphase and early prophase. It then localizes to the basal cortex in late prophase, metaphase, anaphase, and telophase. After mitosis, it segregates into the basal daughter cell.

(Ng et al., 2016, Shen et al., 1998)

stau protein is localized to the apical cortex and cytoplasm in late interphase neuroblasts. From metaphase through to telophase, stau protein is localized in a basal cortical crescent. At cytokinesis, it segregates into the daughter ganglion mother cell (GMC). stau protein localization is not microtubule-dependent.

(Gardiol and St Johnston, 2014, Broadus et al., 1998)

A GFP-stau protein fusion was localized in live oocytes to the anterior region in the same position as the bcd transcript.

(Martin et al., 2003)

In a freshly laid fertilized embryo, stau protein is detected at the anterior and posterior poles. An unfertilized egg displays initial stau protein localization, which diffuses as the egg ages.

(Matsuzaki et al., 1998, Ferrandon et al., 1994)

stau protein localizes to the apical side of neuroblasts at interphase. During prophase it forms a crescent on the basal side of the cell and remains there through the rest of mitosis. It partitions to the GMC when the cell divides. stau protein is often associated with the centrosome on the apical side of the neuroblast during anaphase. The carboxy-terminal 157 amino acids of stau are required for its localization to the cortex in neuroblasts.

(Schuldt et al., 1998, St. Johnston et al., 1991)

In stage 10 oocytes, vas protein, osk protein, and stau protein colocalize at the posterior pole. In early embryos, vas protein and osk protein localize to the polar granules but stau protein does not colocalize with them. It is present in a thin crescent apposed closely to the posterior cortex and disappears before the pole cell stage. A GFP-Vas fusion protein was used to determine the vas protein distribution.

(Breitwieser et al., 1996)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in apical cortex

inferred from direct assay

(Li et al., 1997)

located_in apical part of cell

inferred from direct assay

(Fuerstenberg et al., 1998)

located_in apical plasma membrane

inferred from direct assay

(Shen et al., 1998)

located_in basal cortex

inferred from direct assay

(Landskron et al., 2018, Fuerstenberg et al., 1998)

located_in basal plasma membrane

inferred from direct assay

(Shen et al., 1998)

located_in cell cortex

inferred from direct assay

(Li et al., 1997)

located_in cytoplasm

inferred from direct assay

(Fuerstenberg et al., 1998)

located_in germ plasm

inferred from direct assay

(assigned by BHF-UCL )

(Li et al., 2009)

part_of messenger ribonucleoprotein complex

inferred from direct assay

(assigned by UniProt )

(Barbee et al., 2006)

located_in neuronal cell body

inferred from direct assay

(assigned by UniProt )

(Hillebrand et al., 2010, Barbee et al., 2006)

located_in neuronal ribonucleoprotein granule

inferred from direct assay

(assigned by UniProt )

(Hillebrand et al., 2010, Barbee et al., 2006)

located_in P-body

inferred from direct assay

(Eulalio et al., 2007)

located_in subsynaptic reticulum

inferred from direct assay

(Gardiol and St Johnston, 2014)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\stau in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\stau

View exonic expression by tissue for Dmel\stau

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003520

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0003520

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5753-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5753

Flygut - An atlas of the Drosophila adult midgut

FBgn0003520

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\stau

Stages(s) 4-6
Stages(s) 11-12
Stages(s) 13-16

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 20 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 29 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of stau

Transgenic constructs containing regulatory region of stau

Deletions and Duplications ( 24 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | maternal effect

stau^unspecified

lethal | maternal effect | recessive

lethal | maternal effect | recessive | heat sensitive

stau³

lethal | non-rescuable maternal effect

stau¹

lethal - all die before end of embryonic stage | germline clone | non-rescuable maternal effect | recessive

lethal - all die before end of embryonic stage | maternal effect | recessive

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

stau^GD11758

Sterility

Allele

female sterile (with Df(2R)BSC334)

stau¹

female sterile (with Df(2R)ED3610)

stau¹

female sterile | maternal effect

female sterile | recessive

stau^unspecified

stau¹

grandchildless | recessive

stau^unspecified

male sterile | maternal effect

Other Phenotypes

Allele

memory defective | heat sensitive (with stau²)

stau³

memory defective | heat sensitive (with stau³)

stau²

neuroanatomy defective | somatic clone, with Scer\FLP1^UAS.cUa, Scer\GAL4^Act.PU, Scer\GAL4^eve.RRK

stau^G2796

neuroanatomy defective | third instar larval stage (with Df(2R)Pcl7B)

some die during embryonic stage | non-rescuable maternal effect | recessive

some die during embryonic stage | recessive | maternal effect | recessive

some die during pupal stage, with Scer\GAL4^pnr-MD237

stau^GD11758

Phenotype manifest in

Allele

abdominal segment

stau¹

cephalic furrow | maternal effect

stau^unspecified

dendrite | somatic clone, with Scer\FLP1^UAS.cUa, Scer\GAL4^Act.PU, Scer\GAL4^eve.RRK

stau^G2796

dorsal arm of pharyngeal sclerite | maternal effect (with Df(2R)PC4)

stau¹

embryo | germline clone | maternal effect | posterior

embryonic/larval neuromuscular junction | third instar larval stage (with Df(2R)Pcl7B)

embryonic abdomen

embryonic abdomen | maternal effect

stau^ry9

embryonic abdominal segment 1

stau^unspecified

embryonic abdominal segment 2

stau^unspecified

embryonic abdominal segment 3

stau^unspecified

embryonic abdominal segment 4

stau^unspecified

embryonic abdominal segment 5

stau^unspecified

embryonic abdominal segment 6

stau^unspecified

embryonic abdominal segment 7

stau^unspecified

embryonic abdominal segment 8

stau^unspecified

embryonic abdominal segment 9

stau^unspecified

embryonic abdominal segment 10

stau^unspecified

embryonic abdominal segment 11

stau^unspecified

embryonic abdominal segment | maternal effect

stau^unspecified

embryonic abdominal segment | maternal effect (with Df(2R)PC4)

stau¹

embryonic abdominal segment | non-rescuable maternal effect

stau¹

embryonic head | anterior

stau^unspecified

embryonic head | anterior | maternal effect

stau^unspecified

embryonic head | germline clone | maternal effect

embryonic head | maternal effect

stau^ry9

embryonic labral segment | non-rescuable maternal effect

stau¹

median tooth | maternal effect (with Df(2R)PC4)

stau¹

pole granule | maternal effect

stau^unspecified

stau^unspecified

primordial germ cell

primordial germ cell | maternal effect

stau^unspecified

primordial germ cell | non-rescuable maternal effect

stau¹

RP2 motor neuron | somatic clone, with Scer\FLP1^UAS.cUa, Scer\GAL4^Act.PU, Scer\GAL4^eve.RRK

stau^G2796

type Ib terminal bouton | third instar larval stage (with Df(2R)Pcl7B)

ventral arm of pharyngeal sclerite | maternal effect (with Df(2R)PC4)

stau¹

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

11 of 15

Yes

Yes

10 of 15

No

Yes

2 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

9 of 15

Yes

Yes

9 of 15

Yes

Yes

2 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Rattus norvegicus (Norway rat) (6)

7 of 13

Yes

Yes

5 of 13

No

Yes

2 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Xenopus tropicalis (Western clawed frog) (4)

9 of 12

Yes

Yes

5 of 12

No

Yes

2 of 12

No

No

1 of 12

No

No

Danio rerio (Zebrafish) (6)

10 of 15

Yes

Yes

10 of 15

Yes

Yes

2 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Caenorhabditis elegans (Nematode, roundworm) (2)

12 of 15

Yes

Yes

1 of 15

No

Yes

Arabidopsis thaliana (thale-cress) (1)

1 of 9

Yes

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091906QT )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00003128

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150495 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_13803

Mayetiola destructor

Hessian fly

Mdes\Mdes012280

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL007470

Anopheles darlingi

American malaria mosquito

Adar\ADAC002263

Anopheles gambiae

Malaria mosquito

Agam\AGAP007478

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ000296

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05H4 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA002695

Danaus plexippus

Monarch butterfly

Dple\DPOGS201522-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL015506

Apis florea

Little honeybee

Aflo\2345803364

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002503

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG013994

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_08157

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM616960

Rhodnius prolixus

Kissing bug

Rpro\RPRC010019

Cimex lectularius

Bed bug

Clec\CLEC007684

Cimex lectularius

Bed bug

Clec\CLEC007684-PB

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008002

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_08079

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X04V8 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR004496

Ixodes scapularis

Black-legged tick

Isca\ISCW004051

Stegodyphus mimosarum

African social velvet spider

Smim\KFM77620.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur29g00400

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_321006

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_324210

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G07A0 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Staufen1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Staufen2

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (8)

Gene Symbol

Score

Source

Alignment

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN8OS^CTG112.UAS

(Mutsuddi et al., 2004)

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN8OS^CTG112.UAS

(Mutsuddi et al., 2004)

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN8OS^CTG112.UAS

(Mutsuddi et al., 2004)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

STAU2; staufen double-stranded RNA binding protein 2

11 of 15

STAU1; staufen double-stranded RNA binding protein 1

10 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization, anti tag coimmunoprecipitation, western blot

(Lu et al., 2018, Laver et al., 2013)

anti tag coimmunoprecipitation, western blot

(Lu et al., 2018)

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Bansal et al., 2020)

anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry

(Bansal et al., 2020)

anti tag coimmunoprecipitation, western blot

(Gonsalvez et al., 2010)

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Landskron et al., 2018)

anti tag coimmunoprecipitation, western blot

(Gáspár et al., 2017)

anti bait coimmunoprecipitation, western blot

(Wodarz et al., 1999)

pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, primer specific pcr

(Jia et al., 2015, Loedige et al., 2015)

pull down, western blot, anti bait coimmunoprecipitation

(Piccioni et al., 2009)

anti tag coimmunoprecipitation, western blot

(Lu et al., 2018)

anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry

(Bansal et al., 2020)

two hybrid, pull down, autoradiography

(Li et al., 1997)

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation

(Gaspar et al., 2014)

anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry

(Bansal et al., 2020)

anti bait coimmunoprecipitation, western blot, isothermal titration calorimetry, predetermined participant, pull down, autoradiography, two hybrid, molecular sieving, x-ray crystallography, anti tag coimmunoprecipitation, anti tag western blot

(Jia et al., 2015, Petritsch et al., 2003, Schuldt et al., 1998, Shen et al., 1998)

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, two hybrid, pull down, autoradiography, anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Lu et al., 2018, Gaspar et al., 2014, Laver et al., 2013, Breitwieser et al., 1996)

RNA-protein

Physical Interaction

Assay

References

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization, anti tag coimmunoprecipitation, western blot

(Lu et al., 2018, Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization, anti tag coimmunoprecipitation

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization, anti tag coimmunoprecipitation

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization, anti tag coimmunoprecipitation

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization, anti tag coimmunoprecipitation

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization, anti tag coimmunoprecipitation

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Mahoney et al., 2016)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization, anti tag coimmunoprecipitation

(Laver et al., 2013)

stau - VhaPPA1-1

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization, primer specific pcr, anti tag coimmunoprecipitation, rna three hybrid, fluorescence polarization spectroscopy, predetermined participant

(Lazzaretti et al., 2018, Laver et al., 2013, Laver et al., 2012, Irion and St Johnston, 2007)

pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, primer specific pcr

(Jia et al., 2015, Loedige et al., 2015)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013)

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Mahoney et al., 2016)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization, anti tag coimmunoprecipitation

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

stau - hpRNA:CR18854

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization, anti tag coimmunoprecipitation

(Laver et al., 2013)

stau - hpRNA:CR32207

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization, anti tag coimmunoprecipitation

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

stau - l(3)neo38

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

stau - lncRNA:cherub

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Landskron et al., 2018)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, two hybrid, pull down, autoradiography, anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Lu et al., 2018, Gaspar et al., 2014, Laver et al., 2013, Breitwieser et al., 1996)

pull down, autoradiography, anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013, Li et al., 1997)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

RNA-RNA

Physical Interaction

Assay

References

luminiscence technology

(Iovino et al., 2009)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Navarro et al., 2004)

suppressible

(Navarro et al., 2004)

suppressible

(Navarro et al., 2004)

enhanceable

(Bolduc et al., 2008)

suppressible

(Navarro et al., 2004)

enhanceable

(Ruden et al., 1997)

suppressible

stau::Mdoa\stau

(Micklem et al., 2000)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Timmerman et al., 2013)

suppressible

(Navarro et al., 2004)

suppressible

(Navarro et al., 2004)

suppressible

(Navarro et al., 2004)

suppressible

(Navarro et al., 2004)

suppressible

(Ida et al., 2009)

enhanceable

(Bolduc et al., 2008)

enhanceable

(Fan et al., 2011)

suppressible

(Irion et al., 2006)

enhanceable

(Ruden et al., 1997)

suppressible

(Micklem et al., 2000)

External Data

Subunit Structure (UniProtKB)

Component of neuronal ribonucleoprotein complexes (RNPs) that contains at least various translational repressor and mRNA turnover proteins such as me31B, tral, Upf1, AGO2 and sometimes Fmr1.

(UniProt, P25159 )

Linkouts

BioGRID - A database of protein and genetic interactions.

62749

DroID - A comprehensive database of gene and protein interactions.

FBgn0003520

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37065

MIST (protein-protein) - An integrated Molecular Interaction Database

stau

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2R

Recombination map

2-84

Cytogenetic map

Sequence location

2R:18,117,896..18,125,779 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

55B5-55B7

Limits computationally determined from genome sequence between P{PZ}grh⁰⁶⁸⁵⁰&P{lacW}olf186-F^k11505 and P{PZ}Hsf⁰³⁰⁹¹&P{EP}Dgp-1^EP731

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

55A-55B

(determined by in situ hybridisation)

(St. Johnston et al., 1991)

Experimentally Determined Recombination Data

Location

2-84

(FlyBase, 2016)

2-88.7

(Comeron et al., 2012)

2-86

(Schupbach and Wieschaus, 1989)

2-83.5

(Tearle and Nusslein-Volhard, 1987)

Left of (cM)

Right of (cM)

c

(Doane, 1999)

Notes

Stocks and Reagents

Stocks (19)

86573

cn¹ stau²/CyO

86576

w^*; stau^r9/SM6a

1507

stau¹ cn¹ bw¹/CyO

31247

y¹ v¹; P{TRiP.JF01764}attP2

27013

y¹ w^*; P{EP}stau^G2796

86572

stau² PBac{αTub67C-stau.PB}VK00037/SM6a

86571

stau² PBac{αTub67C-stau.PA.mGFP6}VK00037/SM6a

104387

w^*; P{GawB}stau^NP3132

106354

stau¹ cn¹ bw¹/CyO

v330243

P{VSH330243}attP40

v106645

P{KK108121}VIE-260B

v318898

PBac{fTRG01404.sfGFP-TVPTBF}VK00033

More stocks available...

Genomic Clones (22)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (102)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003520

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003520

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37065

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Laver et al., 2012)

polyclonal

(Krauss et al., 2009, St. Johnston et al., 1991)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: stau CG5753

(Li, 1999.11)

Source for database merge of

Additional comments

Other Comments

stau is required for localisation of cora mRNA and protein at the postsynaptic side of the neuromuscular junction.

(Gardiol and St Johnston, 2014)

stau has a role in long-term memory.

(Dubnau et al., 2003)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

stau is not required for the cell fate decision in the NB6-4T lineage.

(Akiyama-Oda et al., 2000)

Distinct domains of stau mediate microtubule and actin based MRNA transport.

(Micklem et al., 2000)

pros mRNA and the RNA-binding protein stau are asymmetrically localised in mitotic neuroblasts and are specifically partitioned into the GMC, as is pros protein. stau is required for localisation of pros RNA but not of pros protein. Loss of localisation of stau or of pros RNA alters GMC development, but only in embryos with reduced levels of pros protein, suggesting that pros RNA and pros protein act redundantly to specify GMC fate. GMCs do not transcribe the pros gene, showing that inheritance of pros RNA and/or pros proteins from the neuroblast is essential for GMC specification.

(Broadus et al., 1998)

mira function is required for asymmetrical localisation of stau protein.

(Shen et al., 1998)

insc, pros and stau localisation in neuroblasts in vitro is studied. Extrinsic and intrinsic cues and microfilaments regulate the subcellular localisation of the pros, insc and stau proteins in neuroblasts.

(Broadus and Doe, 1997)

Binding of the bcd 3'UTR by stau protein requires a double stranded conformation of the stems within the bcd RNA localisation signal.

(Ferrandon et al., 1997)

insc and stau mediate asymmetric localization and segregation of pros RNA during neuroblast cell divisions. stau protein binds the pros RNA 3'UTR, via stau residues 565-661, which lie amino terminal to and distinct from the region of stau required to interact with the insc protein.

(Li et al., 1997)

osk, stau, vas and tud are essential for pole plasm formation. stau is required for both osk RNA localisation and translation.

(Breitwieser et al., 1996)

Mutants in chic resemble those in capu in that they fail to localize stau protein and osk mRNA to the posterior pole of the developing oocyte.

(Manseau et al., 1996)

Multinuclear/multidimensional NMR methods have been used to determine that the stau double stranded RNA binding domain (dsRBD) 3 forms a compact protein domain with an α-β-β-β-α structure. In vitro mutagenesis has identified F43 and T50 as two residues essential for RNA binding.

(Bycroft et al., 1995)

Nurse cell-specific genes are functional in the pseudonurse cells of otu mutants, but the transport of pum, otu, ovo and bcd RNAs to the cytoplasm is affected.

(Heino et al., 1995)

The stau product associates specifically with both osk and bcd mRNAs to mediate their localizations, but at two distinct stages of development. stau protein is required to anchor bcd mRNA at the anterior of the egg, and is transported with osk mRNA during oogenesis.

(Ferrandon et al., 1994)

Distribution of tud protein in mutant embryos has been studied.

(Bardsley et al., 1993)

Comparison of bcd and hts transcript distribution in swa, exu and stau mutant embryos indicates different genetic requirements for proper localization of bcd and hts RNAs.

(Ding et al., 1993)

The localization of the osk RNA and stau protein in oogenesis do not depend of the product of the psq locus.

(Siegel et al., 1993)

Only vas and tud are essential for osk-induced pole cell and abdomen formation, stau is not.

(Ephrussi and Lehmann, 1992)

vas, vls and tud (but not stau, capu, or spir) are necessary for the 6xosk mutant phenotypes.

(Smith et al., 1992)

Deletion analysis and computer analysis identifies a 65 to 68 amino acid consensus sequence defining the minimal dsDNA binding domain.

(St. Johnston et al., 1992)

stau is required specifically for the localization of the posterior signal in the embryo, not the synthesis of the signal. BicD,stau embryos exhibit a duplication of the posterior telson at the anterior: the posterior signal can be active at the anterior independent of stau function. stau gene function is unique among maternal genes as it is required for both the accumulation of the anterior bcd-dependent signal and the posterior nos-dependent signal.

(Lehmann and Nusslein-Volhard, 1991)

Mutations in maternal posterior class gene stau do not interact with RpII140^wimp.

(Parkhurst and Ish-Horowicz, 1991)

The posterior group gene stau is required both for the localization of maternal determinants to the posterior pole of the egg and for bcd RNA to localize correctly to the anterior pole. The stau product is one of the first molecules to localize to the posterior pole of the oocyte, perhaps in association with osk RNA. Once localized, stau product is found in the polar granules and is required to hold other polar granule components at the posterior pole. By the time the egg is laid, stau protein is also concentrated at the anterior pole, in the same region as bcd RNA.

(St. Johnston et al., 1991)

stau mutants exhibit deletion of the abdomen and pole plasm and anterior head defects.

(Gaul and Jäckle, 1990)

Mature follicles are immunologically stained for asymmetric distribution of ecdysteroid-related antigen. During late oogenesis localisation of the antigen changes dramatically suggesting the antigen plays a role in early embryogenesis and, perhaps, in pattern formation.

(Grau and Gutzeit, 1990)

In stau mutant females vas synthesis appears normal but the vas protein is not localized.

(Hay et al., 1990)

stau plays a role in polar granule formation.

(Raff et al., 1990)

Mutation in stau results in a maternal effect "grandchildless knirps-like" phenotype.

(Schupbach and Wieschaus, 1989)

stau mutations disrupt the fourth stage of bcd RNA localization during oogenesis: localization to a spherical region that occupies a slightly dorsal position of the anterior pole.

(St. Johnston et al., 1989)

Mutations in stau cause failure of germ cell formation and deletions in the abdominal segments in the embryo.

(Schupbach and Wieschaus, 1986)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 51 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37065

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P25159

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

E1JGK6

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003520

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003520

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5753

Flygut - An atlas of the Drosophila adult midgut

FBgn0003520

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37065

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004615

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG5753

modMine - A data warehouse for the modENCODE project

FBgn0003520

PDB - An information portal to biological macromolecular structures

1EKZ
1STU

Linkouts

BioGRID - A database of protein and genetic interactions.

62749

DroID - A comprehensive database of gene and protein interactions.

FBgn0003520

DRSC - Results frm RNAi screens

FBgn0003520

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0003520

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5753-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003520

FlyMine - An integrated database for Drosophila genomics

FBgn0003520

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37065

MIST (protein-protein) - An integrated Molecular Interaction Database

stau

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

CG5753

(Meadows, 2017.1.11, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Ida et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2008, Molnar et al., 2006, Goldstein and Gunawardena, 2000)

Dmstau

(DeSousa et al., 2003)

STAU

(Sofola et al., 2007, Fierro-Monti and Mathews, 2000)

Stau

(Bansal et al., 2020, Dold et al., 2020, Lee et al., 2020, Gáspár et al., 2017, Jansen and Niessing, 2012, Ottone et al., 2012, Parton et al., 2011, Poulton et al., 2011, Sun et al., 2011, Tanaka et al., 2011, Vazquez-Pianzola et al., 2011, Yan et al., 2011, Klusza and Deng, 2010, Krahn et al., 2010, Weil et al., 2010, Yu et al., 2010, Yu et al., 2010, McNeil et al., 2009, Shyu et al., 2009, Snee and Macdonald, 2009, Tian and Deng, 2009, Gervais et al., 2008, Li et al., 2008, Lin et al., 2008, Tanaka and Nakamura, 2008, Tian and Deng, 2008, Weil et al., 2008, Yu et al., 2008, Eulalio et al., 2007, Geng and MacDonald, 2007, Irion and St Johnston, 2007, Januschke et al., 2007, Meignin et al., 2007, Nurminsky, 2007, Polesello and Tapon, 2007, Sofola et al., 2007, Song et al., 2007, Geng and Macdonald, 2006, Poulton and Deng, 2006, Yucel and Small, 2006, Minakhina and Steward, 2005)

Stauf

(Slack et al., 2007)

Staufen

(Landskron et al., 2018)

dStau

(Macchi et al., 2003, Daviet et al., 2000)

stau

(Bono, 2020.3.10, Eichler et al., 2020, Lasko, 2020, Yang et al., 2020, Tiwari et al., 2019, Trcek and Lehmann, 2019, Whittle and Extavour, 2019, Lazzaretti et al., 2018, Cao et al., 2016, Ng et al., 2016, Sanghavi et al., 2016, Sarov et al., 2016, Schwartz et al., 2016, Jambor et al., 2015, Jia et al., 2015, Kavi et al., 2015, Little et al., 2015, Loedige et al., 2015, Gardiol and St Johnston, 2014, Williams et al., 2014, Laver et al., 2013, Morais-de-Sá et al., 2013, Sanghavi et al., 2012, Fan et al., 2011, Chang et al., 2010, Gonsalvez et al., 2010, Besse et al., 2009, Ida et al., 2009, Iovino et al., 2009, Kang et al., 2009, Li et al., 2009, Mhlanga et al., 2009, Sousa-Nunes et al., 2009, Spirov et al., 2009, Venken et al., 2009, Bolduc et al., 2008, Christensen et al., 2008.4.15, He et al., 2008, Zimyanin et al., 2008, Bakal et al., 2007, Christensen et al., 2007.10.29, Irion and St Johnston, 2007, Serbus and Sullivan, 2007, Snee et al., 2007, Sofola et al., 2007, Stark et al., 2007, Ashraf et al., 2006, Doerflinger et al., 2006, Lin et al., 2006, Molnar et al., 2006, Munro et al., 2006, Ni et al., 2006, Weil et al., 2006, Navarro et al., 2004, Hall, 2003, Wang et al., 1994)

Name Synonyms

STAUFEN

(Beraud et al., 2007)

Staufen

(Lu et al., 2020, Baskar et al., 2019, Gáspár et al., 2017, Nieuwburg et al., 2017, Mahoney et al., 2016, Nashchekin et al., 2016, Gardiol and St Johnston, 2014, Gaspar et al., 2014, Ghosh et al., 2014, Rumpf et al., 2014, Leibfried et al., 2013, Timmerman et al., 2013, Jansen and Niessing, 2012, Kelsom and Lu, 2012, Ottone et al., 2012, Sanghavi et al., 2012, Parton et al., 2011, Poulton et al., 2011, Sun et al., 2011, Tanaka et al., 2011, van Eyk et al., 2011, Vazquez-Pianzola et al., 2011, Yan et al., 2011, Doerflinger et al., 2010, Gonsalvez et al., 2010, Hillebrand et al., 2010, Klusza and Deng, 2010, Krahn et al., 2010, Kim et al., 2009, Krauss et al., 2009, Krauss et al., 2009, McNeil et al., 2009, Mhlanga et al., 2009, Piccioni et al., 2009, Shyu et al., 2009, Snee and Macdonald, 2009, Tian and Deng, 2009, Boylan et al., 2008, Chia et al., 2008, Gervais et al., 2008, Li et al., 2008, Lin, 2008, Lin et al., 2008, Sousa-Nunes et al., 2008, Sung et al., 2008, Weil et al., 2008, Yu et al., 2008, Zimyanin et al., 2008, Barbosa et al., 2007, Coutelis and Ephrussi, 2007, Eulalio et al., 2007, Irion and St Johnston, 2007, Klusza and Deng, 2007, Meignin et al., 2007, Mische et al., 2007, Polesello and Tapon, 2007, Rusten and Stenmark, 2007, Slack et al., 2007, Weil et al., 2007, Zimyanin et al., 2007, Zimyanin et al., 2007, Barbee et al., 2006, Betschinger et al., 2006, Choksi et al., 2006, Denman, 2006, Doerflinger et al., 2006, Geng and Macdonald, 2006, Irion et al., 2006, Jenny et al., 2006, Kiebler and Bassell, 2006, Poulton and Deng, 2006, Shav-Tal and Singer, 2005)

Stauffen

(Dollar et al., 2002)

staufen

(Davis, 2015, Cheung et al., 2014, Morais-de-Sá et al., 2014, Laver et al., 2013, Miyashita et al., 2012, Winbush et al., 2012, Furman and Bukharina, 2011, Jambor et al., 2011, Loiseau et al., 2010, Weil et al., 2010, Besse et al., 2009, Kang et al., 2009, Spirov et al., 2009, Bolduc et al., 2008, He et al., 2008, Stark et al., 2007, Yucel and Small, 2006, Hall, 2003, Fuerstenberg et al., 1998)

Secondary FlyBase IDs

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

transgene_used

Stau_RIP_interaction_map

interactome

Interaction map generated by purification of stau, with identificaiton of copurifying RNA transcripts by microarray.

References (518)