Gene Dmel\stg

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General Information

Symbol

Dmel\stg

Species

D. melanogaster

Name

string

Annotation symbol

CG1395

Feature type

protein_coding_gene

FlyBase ID

FBgn0003525

Gene Model Status

Current

Stock availability

27 publicly available

Also Known As

cdc25, cdc25^string, l(3)01235, Cdc25^stg, stg^cdc25, string

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

99A5-99A5

Sequence location

3R:25,077,548..25,081,522 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene string is referred to in FlyBase by the symbol Dmel\stg (CG1395, FBgn0003525). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein tyrosine phosphatase activity. There is experimental evidence for 16 unique biological process terms, many of which group under: biological regulation; cellular component organization or biogenesis; cellular process; cell cycle; positive regulation of biological process; cell cycle process; regulation of anatomical structure size; organelle organization; cell proliferation; macromolecule modification; positive regulation of cell proliferation; cell fate commitment; single-organism developmental process. 135 alleles are reported. The phenotypes of these alleles are annotated with: organ system; organ system subdivision; multicellular structure; adult segment; imaginal precursor; sense organ; external compound sense organ; thoracic segment; nuclear part; embryonic/larval hemocoel; eo support cell; embryonic/larval neuron; cell cycle. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: M-phase inducer phosphatase; Rhodanese-like domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval trachea, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1633174_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of stg. Gene sequence location is 3R:25077548..25081522.

User Contributed Data

Beta version, feedback welcome

External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

stg: string

Homozygous embryonic lethal; denticle bands reduced. First 13 nuclear divisions of zygote proceed on schedule; division 14 permanently arrested in G2 in homozygotes for strong alleles; no evidence of nuclear-envelope breakdown or chromosome condensation; division 14 severely impaired in weak alleles. DNA synthesis in arrested embryos confined to the polyploid amnioserosal cells. Gastrulation proceeds on schedule in stg embryos with but 5,000 cells, and markers ordinarily expressed in normal embryos with 50,000 cells also expressed in stg embryos. The only defect seems to be in the initiation of the first mitotic division that is under zygotic control. Clones of homozygous cells induced early don't survive; few late clones produced.

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

Update Feed

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FB2013_03

Alleles

stg[DY]

stg[MI08204]

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

99A5-99A5

Limits computationally determined from genome sequence between P{PZ}l(3)04708⁰⁴⁷⁰⁸ and P{PZ}l(3)06743⁰⁶⁷⁴³

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

99A5-99A7

(determined by in situ hybridisation)

(Ranz et al., 2001)

99A6-99A6

(Prokopenko, 1999.8.3)

99A5-99A6

(determined by in situ hybridisation)

(Spradling et al., 1999)

99A-99A

(determined by in situ hybridisation) 99A1--4 (determined by in situ hybridisation) 99A1--8 (determined by in situ hybridisation)

(Szeged Stock Center, 1998-)

98F-99A

(determined by in situ hybridisation) 99A (determined by in situ hybridisation) 99A1--4 (determined by in situ hybridisation)

(Salzberg et al., 1997)

99A1-99A4

(determined by in situ hybridisation) 99A (determined by in situ hybridisation) 99A1--8 (determined by in situ hybridisation) 98F3--11 (determined by in situ hybridisation)

(Deak et al., 1997)

99A5-99A6

(determined by in situ hybridisation)

(Perrimon et al., 1996)

99A1-99A11

(BDGP Project Members, 1994-1999)

99A-99A

(determined by in situ hybridisation)

(Berg and Spradling, 1991)

98F-99A

(determined by in situ hybridisation)

(Edgar and O'Farrell, 1989)

99A-99A

(determined by in situ hybridisation)

(Cooley et al., 1988)

99A-99A

(Tearle and Nusslein-Volhard, 1987)

Experimentally Determined Recombination Data

Location

3-99

(Tearle and Nusslein-Volhard, 1987, Dong et al., 1997)

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\stg for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

stg-RA

FBtr0085397

NM_079823

3064

479

stg-RB

FBtr0334867

NM_001276137

2747

479

Additional Transcript Data & Comments

Reported size (kB)

2.8 (northern blot)

(Jimenez et al., 1990)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

stg-PA

FBpp0084766

54.1

479

6.93

NP_524547

AAF56885

stg-PB

FBpp0306890

54.1

479

6.93

NP_001263066

AGB96446

Additional Polypeptide Data & Comments

Reported size (kDa)

479 (aa)

(Jimenez et al., 1990)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

M-phase inducer phosphatase (IPR000751)

Rhodanese-like domain (IPR001763)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P20483

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

regulatory region

FBsf0000435027
3R:25,103,167..25,110,404

evidence=Reporter construct (in vivo)

(Lehman et al., 1999, Halfon, 2012.6.19)

regulatory region

FBsf0000435161
3R:25,097,298..25,103,162

evidence=Reporter construct (in vivo)

(Lehman et al., 1999, Halfon, 2012.6.19)

regulatory region

FBsf0000435583
3R:25,088,623..25,089,791

evidence=Reporter construct (in vivo)

(Lehman et al., 1999, Halfon, 2012.6.19)

regulatory region

FBsf0000435857
3R:25,090,536..25,096,418

evidence=Reporter construct (in vivo)

(Lehman et al., 1999, Halfon, 2012.6.19)

regulatory region

FBsf0000436163
3R:25,088,628..25,091,028

evidence=Reporter construct (in vivo)

(Lehman et al., 1999, Halfon, 2012.6.19)

regulatory region

FBsf0000436229
3R:25,087,453..25,094,062

evidence=Reporter construct (in vivo)

(Lehman et al., 1999, Halfon, 2012.6.19)

regulatory region

FBsf0000436435
3R:25,082,448..25,087,448

evidence=Reporter construct (in vivo)

(Lehman et al., 1999, Halfon, 2012.6.19)

regulatory region

FBsf0000436461
3R:25,081,576..25,090,541

evidence=Reporter construct (in vivo)

(Lehman et al., 1999, Halfon, 2012.6.19)

regulatory region

FBsf0000436504
3R:25,094,578..25,097,298

evidence=Reporter construct (in vivo)

(Lehman et al., 1999, Halfon, 2012.6.19)

regulatory region

FBsf0000436560
3R:25,080,102..25,082,453

evidence=Reporter construct (in vivo)

(Lehman et al., 1999, Halfon, 2012.6.19)

regulatory region

FBsf0000436566
3R:25,090,483..25,091,482

evidence=Reporter construct (in vivo)

(Rouault et al., 2010, Halfon, 2012.6.19)

regulatory region

FBsf0000436787
3R:25,076,160..25,082,112

evidence=Rescue construct

(Lehman et al., 1999, Halfon, 2012.6.19)

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

optic lobe

(Alphey et al., 1992)

adult stage | female

(Jimenez et al., 1990)

adult stage & oogenesis

oocyte

(Alphey et al., 1992)

spermatogenesis & adult stage

testis

(Alphey et al., 1992)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage | early

(Alphey et al., 1992)

embryonic stage 1 -- 3

organism

(Fisher et al., 2012)

Comment:maternally deposited

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic cycle 12

organism | ubiquitous

(Lehman et al., 1999)

Comment:maternally deposited

embryonic stage 5

organism | ventral

(Mata et al., 2000)

embryonic stage 6

mitotic domain 1 -- mitotic domain 10

(Lehman et al., 1999)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 8

mitotic domain 1 -- mitotic domain 14

(Lehman et al., 1999)

embryonic stage 9

ventral neurectoderm intermediate zone 1 -- mitotic domain 25

(Lehman et al., 1999)

mitotic domain N

(Lehman et al., 1999)

embryonic epidermis | lateral

(Lehman et al., 1999)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 11

embryonic head

(Lehman et al., 1999)

embryonic stage 10

mitotic domain M

(Lehman et al., 1999)

embryonic ventral epidermis | lateral

(Lehman et al., 1999)

embryonic neuroblast

(Lehman et al., 1999)

embryonic stage 10 -- 11

ventral neurectoderm

(Lehman et al., 1999)

embryonic stage 10 -- 17

Malpighian tubule | restricted

(Kerber et al., 1998)

embryonic stage 11

tracheal primordium

(Lehman et al., 1999)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 12

embryonic epidermis

(Lehman et al., 1999)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval circulatory system

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

larval stage

eye-antennal disc

(Alphey et al., 1992)

adult stage & oogenesis

nurse cell

(Alphey et al., 1992)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage | early

(Jimenez et al., 1990)

Additional Descriptive Data

During Malpighian tubule development, stg is expressed asymmetrically in everting tubules, and subsequently in the distal proliferation zone.

(Kerber et al., 1998)

stg transcripts are expressed maximally in nurse cells in the later stages of oogenesis and the transcripts are transferred to the oocyte from stage 11 onward. The maternally derived transcripts are uniformly distributed in the cytoplasm of the early embryo and are excluded from the forming cells during cellularization. Zygotic expression of stg initiates at cycle 14 and has been described elsewhere (FBrf 49340). stg transcripts are expressed in a variety of proliferating cells and are shown in a broad band that anticipates the mitotic divisions as the morphogenetic furrow passes aacross the eye-entennal disc and in proliferating cells in the optic lobes of the brain. stg transcripts are also expressed in the apical cells of the male testis.

(Alphey et al., 1992)

stg transcripts are most abundant in early embryos and adult females.

(Jimenez et al., 1990)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

cell | subset

(Edgar and Datar, 1996)

Comment:S, G2 and M cycle cells only

Additional Descriptive Data

stg accumulates in S, G2 and M cell cycle phases, but not in G1 phase.

(Edgar and Datar, 1996)

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

chromosome

inferred from direct assay

(Edgar et al., 1994)

nucleus

inferred from direct assay

(Edgar et al., 1994)

Expression Deduced from Reporters

Reporter: P{lacW}S096705a

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval peripheral nervous system | restricted

(Salzberg et al., 1997)

presumptive embryonic/larval central nervous system

(Salzberg et al., 1997)

Reporter: P{lacW}stg^S022406

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Salzberg et al., 1997)

Reporter: P{lacW}stg^S024503

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Salzberg et al., 1997)

Reporter: P{lacW}stg^S024532

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Salzberg et al., 1997)

Reporter: P{lacW}stg^S043922

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Salzberg et al., 1997)

Reporter: P{lacW}stg^S073013

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Salzberg et al., 1997)

Reporter: P{lacW}stg^S089605a

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval central nervous system

(Salzberg et al., 1997)

ectoderm

(Salzberg et al., 1997)

Reporter: P{lacW}stg^S098006

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval peripheral nervous system | restricted

(Salzberg et al., 1997)

ventral neurectoderm

(Salzberg et al., 1997)

Reporter: P{lacW}stg^S106006a

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Salzberg et al., 1997)

Reporter: P{lacW}stg^S108908

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Salzberg et al., 1997)

Reporter: P{stg-lacZ.β-E4.9}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage | early

mesoderm

(Lehman et al., 1999)

embryonic head

(Lehman et al., 1999)

embryonic head

(FlyBase, 1992-)

embryonic neuroblast

(FlyBase, 1992-)

presumptive embryonic/larval peripheral nervous system

(Lehman et al., 1999)

mesoderm

(FlyBase, 1992-)

embryonic epidermis | ventral

(FlyBase, 1992-)

ventral neurectoderm

(Lehman et al., 1999)

presumptive embryonic/larval peripheral nervous system

(FlyBase, 1992-)

embryonic epidermis | ventral

(Lehman et al., 1999)

mesectoderm

(FlyBase, 1992-)

mesectoderm

(Lehman et al., 1999)

embryonic neuroblast

(Lehman et al., 1999)

ventral neurectoderm

(FlyBase, 1992-)

embryonic cycle 1 -- embryonic cycle 13

(Lehman et al., 1999)

(FlyBase, 1992-)

embryonic cycle 12

organism | ubiquitous

(Lehman et al., 1999)

Comment:maternally deposited

embryonic stage 8

mitotic domain 10

(Lehman et al., 1999)

mesoderm

(Lehman et al., 1999)

mitotic domain 14

(Lehman et al., 1999)

mesectoderm

(Lehman et al., 1999)

embryonic stage 8 -- 9

ventral neurectoderm intermediate zone 1

(Lehman et al., 1999)

embryonic stage 8 -- 10

ventral neurectoderm

(Lehman et al., 1999)

embryonic stage 9

mitotic domain N

(Lehman et al., 1999)

ventral neurectoderm intermediate zone 1

(Lehman et al., 1999)

embryonic stage 9 -- 10

mitotic domain M

(Lehman et al., 1999)

embryonic ventral epidermis

(Lehman et al., 1999)

embryonic neuroblast

(Lehman et al., 1999)

embryonic stage 10

embryonic head

(Lehman et al., 1999)

larval stage

embryonic/larval central nervous system

(Lehman et al., 1999)

neuroblast

(FlyBase, 1992-)

embryonic/larval central nervous system

(FlyBase, 1992-)

neuroblast

(Lehman et al., 1999)

second instar larval stage

larval outer optic anlage

(Lehman et al., 1999)

second instar larval stage -- third instar larval stage

neuroblast of embryonic/larval brain

(Lehman et al., 1999)

Comment:central brain neuroblast

third instar larval stage

larval outer optic anlage | anterior & posterior

(Lehman et al., 1999)

adult stage oogenesis stage S1 -- S2

germarium

(Deng et al., 2001)

follicle cell

(Deng et al., 2001)

Reporter: P{stg-lacZ.β}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage | early

presumptive embryonic/larval central nervous system

(Lehman et al., 1999)

embryonic neuroblast

(Lehman et al., 1999)

embryonic epidermis | lateral

(Lehman et al., 1999)

larval stage

optic lobe

(Lehman et al., 1999)

embryonic/larval central nervous system

(Lehman et al., 1999)

neuroblast

(Lehman et al., 1999)

adult stage & oogenesis

germline stem cell

(Deng et al., 2001)

Reporter: P{stg-lacZ.HZ-B8.2}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage | early

mesoderm

(Lehman et al., 1999)

embryonic head

(Lehman et al., 1999)

embryonic head

(FlyBase, 1992-)

tracheal primordium

(Lehman et al., 1999)

mesoderm

(FlyBase, 1992-)

presumptive embryonic/larval central nervous system

(FlyBase, 1992-)

embryonic epidermis | ventral

(FlyBase, 1992-)

neuroblast

(Lehman et al., 1999)

ventral neurectoderm

(Lehman et al., 1999)

embryonic epidermis | ventral

(Lehman et al., 1999)

neuroblast

(FlyBase, 1992-)

presumptive embryonic/larval central nervous system

(Lehman et al., 1999)

mesectoderm

(FlyBase, 1992-)

mesectoderm

(Lehman et al., 1999)

ventral neurectoderm

(FlyBase, 1992-)

tracheal primordium

(FlyBase, 1992-)

embryonic cycle 1 -- embryonic cycle 13

(Lehman et al., 1999)

(FlyBase, 1992-)

Reporter: P{stg-lacZ.HZ}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage | early

embryonic neuroblast

(FlyBase, 1992-)

embryonic epidermis | lateral

(FlyBase, 1992-)

presumptive embryonic/larval central nervous system

(Lehman et al., 1999)

presumptive embryonic/larval central nervous system

(FlyBase, 1992-)

embryonic neuroblast

(Lehman et al., 1999)

embryonic epidermis | lateral

(Lehman et al., 1999)

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval trachea, larval carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		852.825
Larval Midgut		33.2
Larval Hindgut		47.7
Larval Malpighian Tubules		10.7
Larval Fat Body		27
Larval Salivary Gland		16.1
Larval Trachea		284.175
Larval Carcass		108.725
Adult Head		13.7
Adult Eye		5.325
Adult Brain		6.9
Adult Thoracic-Abdominal Ganglion		7.8
Adult Crop		11.9
Adult Midgut		6.9
Adult Hindgut		7.1
Adult Malpighian Tubules		9.9
Adult Fat Body		27.4
Adult Salivary Gland		13.9
Adult Heart		25.075
Adult VirginFemale Spermatheca		18.4
Adult InseminatedFemale Spermatheca		17.7
Adult Ovary		1774.4
Adult Testis		5.4
Adult Male Accessory Gland		18.2
Adult Carcass		30.2
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(852.825)
Larval Midgut		33.2
Larval Hindgut		47.7
Larval Malpighian Tubules		10.7
Larval Fat Body		27
Larval Salivary Gland		16.1
Larval Trachea		284.175
Larval Carcass		108.725
Adult Head		13.7
Adult Eye		5.325
Adult Brain		6.9
Adult Thoracic-Abdominal Ganglion		7.8
Adult Crop		11.9
Adult Midgut		6.9
Adult Hindgut		7.1
Adult Malpighian Tubules		9.9
Adult Fat Body		27.4
Adult Salivary Gland		13.9
Adult Heart		25.075
Adult VirginFemale Spermatheca		18.4
Adult InseminatedFemale Spermatheca		17.7
Adult Ovary		(1774.4)
Adult Testis		5.4
Adult Male Accessory Gland		18.2
Adult Carcass		30.2
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		852.825
Larval Midgut		33.2
Larval Hindgut		47.7
Larval Malpighian Tubules		10.7
Larval Fat Body		27
Larval Salivary Gland		16.1
Larval Trachea		284.175
Larval Carcass		108.725
Adult Head		13.7
Adult Eye		5.325
Adult Brain		6.9
Adult Thoracic-Abdominal Ganglion		7.8
Adult Crop		11.9
Adult Midgut		6.9
Adult Hindgut		7.1
Adult Malpighian Tubules		9.9
Adult Fat Body		27.4
Adult Salivary Gland		13.9
Adult Heart		25.075
Adult VirginFemale Spermatheca		18.4
Adult InseminatedFemale Spermatheca		17.7
Adult Ovary		(1774.4)
Adult Testis		5.4
Adult Male Accessory Gland		18.2
Adult Carcass		30.2
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		852.825
Larval Midgut		33.2
Larval Hindgut		47.7
Larval Malpighian Tubules		10.7
Larval Fat Body		27
Larval Salivary Gland		16.1
Larval Trachea		284.175
Larval Carcass		108.725
Adult Head		13.7
Adult Eye		5.325
Adult Brain		6.9
Adult Thoracic-Abdominal Ganglion		7.8
Adult Crop		11.9
Adult Midgut		6.9
Adult Hindgut		7.1
Adult Malpighian Tubules		9.9
Adult Fat Body		27.4
Adult Salivary Gland		13.9
Adult Heart		25.075
Adult VirginFemale Spermatheca		18.4
Adult InseminatedFemale Spermatheca		17.7
Adult Ovary		1774.4
Adult Testis		5.4
Adult Male Accessory Gland		18.2
Adult Carcass		30.2
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		852.825
Larval Midgut		33.2
Larval Hindgut		47.7
Larval Malpighian Tubules		10.7
Larval Fat Body		27
Larval Salivary Gland		16.1
Larval Trachea		284.175
Larval Carcass		108.725
Adult Head		13.7
Adult Eye		5.325
Adult Brain		6.9
Adult Thoracic-Abdominal Ganglion		7.8
Adult Crop		11.9
Adult Midgut		6.9
Adult Hindgut		7.1
Adult Malpighian Tubules		9.9
Adult Fat Body		27.4
Adult Salivary Gland		13.9
Adult Heart		25.075
Adult VirginFemale Spermatheca		18.4
Adult InseminatedFemale Spermatheca		17.7
Adult Ovary		1774.4
Adult Testis		5.4
Adult Male Accessory Gland		18.2
Adult Carcass		30.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(852.825)
Larval Midgut		33.2
Larval Hindgut		47.7
Larval Malpighian Tubules		10.7
Larval Fat Body		27
Larval Salivary Gland		16.1
Larval Trachea		284.175
Larval Carcass		108.725
Adult Head		13.7
Adult Eye		5.325
Adult Brain		6.9
Adult Thoracic-Abdominal Ganglion		7.8
Adult Crop		11.9
Adult Midgut		6.9
Adult Hindgut		7.1
Adult Malpighian Tubules		9.9
Adult Fat Body		27.4
Adult Salivary Gland		13.9
Adult Heart		25.075
Adult VirginFemale Spermatheca		18.4
Adult InseminatedFemale Spermatheca		17.7
Adult Ovary		(1774.4)
Adult Testis		5.4
Adult Male Accessory Gland		18.2
Adult Carcass		30.2
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		852.825
Larval Midgut		33.2
Larval Hindgut		47.7
Larval Malpighian Tubules		10.7
Larval Fat Body		27
Larval Salivary Gland		16.1
Larval Trachea		284.175
Larval Carcass		108.725
Adult Head		13.7
Adult Eye		5.325
Adult Brain		6.9
Adult Thoracic-Abdominal Ganglion		7.8
Adult Crop		11.9
Adult Midgut		6.9
Adult Hindgut		7.1
Adult Malpighian Tubules		9.9
Adult Fat Body		27.4
Adult Salivary Gland		13.9
Adult Heart		25.075
Adult VirginFemale Spermatheca		18.4
Adult InseminatedFemale Spermatheca		17.7
Adult Ovary		(1774.4)
Adult Testis		5.4
Adult Male Accessory Gland		18.2
Adult Carcass		30.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		852.825
Larval Midgut		33.2
Larval Hindgut		47.7
Larval Malpighian Tubules		10.7
Larval Fat Body		27
Larval Salivary Gland		16.1
Larval Trachea		284.175
Larval Carcass		108.725
Adult Head		13.7
Adult Eye		5.325
Adult Brain		6.9
Adult Thoracic-Abdominal Ganglion		7.8
Adult Crop		11.9
Adult Midgut		6.9
Adult Hindgut		7.1
Adult Malpighian Tubules		9.9
Adult Fat Body		27.4
Adult Salivary Gland		13.9
Adult Heart		25.075
Adult VirginFemale Spermatheca		18.4
Adult InseminatedFemale Spermatheca		17.7
Adult Ovary		1774.4
Adult Testis		5.4
Adult Male Accessory Gland		18.2
Adult Carcass		30.2
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	13.7
NA	Eye	5.325
NA	Brain	6.9
852.825	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	7.8
NA	Crop	11.9
33.2	Midgut	6.9
47.7	Hindgut	7.1
no informative data	Malpighian Tubules	9.9
27	Fat Body	27.4
16.1	Salivary Gland	no informative data
NA	Heart	25.075
284.175	Trachea	NA
NA	VirginFemale Spermatheca	no informative data
NA	InseminatedFemale Spermatheca	no informative data
NA	Ovary	1774.4
NA	Testis	5.4
NA	Male Accessory Gland	no informative data
108.725	Carcass	no informative data

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		57
central nervous system, larvae L3		87
central nervous system, pupae P8		6
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		23
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		3
digestive system, 4-day adult		11
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		9
fat body, pupae P8		17
carcass, larvae L3 wandering		34
carcass, 1-day adult		6
carcass, 4-day adult		16
carcass, 20-day adult		13
ovary, virgin 4-day female		284
ovary, mated 4-day female		252
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(57)
central nervous system, larvae L3		(87)
central nervous system, pupae P8		6
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		23
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		3
digestive system, 4-day adult		11
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		9
fat body, pupae P8		17
carcass, larvae L3 wandering		(34)
carcass, 1-day adult		6
carcass, 4-day adult		16
carcass, 20-day adult		13
ovary, virgin 4-day female		(284)
ovary, mated 4-day female		(252)
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		57
central nervous system, larvae L3		87
central nervous system, pupae P8		6
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		23
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		3
digestive system, 4-day adult		11
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		9
fat body, pupae P8		17
carcass, larvae L3 wandering		34
carcass, 1-day adult		6
carcass, 4-day adult		16
carcass, 20-day adult		13
ovary, virgin 4-day female		(284)
ovary, mated 4-day female		(252)
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		57
central nervous system, larvae L3		87
central nervous system, pupae P8		6
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		23
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		3
digestive system, 4-day adult		11
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		9
fat body, pupae P8		17
carcass, larvae L3 wandering		34
carcass, 1-day adult		6
carcass, 4-day adult		16
carcass, 20-day adult		13
ovary, virgin 4-day female		284
ovary, mated 4-day female		252
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		57
central nervous system, larvae L3		87
central nervous system, pupae P8		6
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		23
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		3
digestive system, 4-day adult		11
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		9
fat body, pupae P8		17
carcass, larvae L3 wandering		34
carcass, 1-day adult		6
carcass, 4-day adult		16
carcass, 20-day adult		13
ovary, virgin 4-day female		284
ovary, mated 4-day female		252
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(57)
central nervous system, larvae L3		(87)
central nervous system, pupae P8		6
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		23
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		3
digestive system, 4-day adult		11
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		9
fat body, pupae P8		17
carcass, larvae L3 wandering		(34)
carcass, 1-day adult		6
carcass, 4-day adult		16
carcass, 20-day adult		13
ovary, virgin 4-day female		(284)
ovary, mated 4-day female		(252)
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		57
central nervous system, larvae L3		87
central nervous system, pupae P8		6
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		23
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		3
digestive system, 4-day adult		11
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		9
fat body, pupae P8		17
carcass, larvae L3 wandering		34
carcass, 1-day adult		6
carcass, 4-day adult		16
carcass, 20-day adult		13
ovary, virgin 4-day female		(284)
ovary, mated 4-day female		(252)
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		57
central nervous system, larvae L3		87
central nervous system, pupae P8		6
head, virgin 1-day female		1
head, virgin 4-day female		1
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		1
head, mated 20-day female		1
head, mated 1-day male		1
head, mated 4-day male		1
head, mated 20-day male		1
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		23
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		3
digestive system, 4-day adult		11
digestive system, 20-day adult		6
fat body, larvae L3 wandering		1
fat body, white prepupae		9
fat body, pupae P8		17
carcass, larvae L3 wandering		34
carcass, 1-day adult		6
carcass, 4-day adult		16
carcass, 20-day adult		13
ovary, virgin 4-day female		284
ovary, mated 4-day female		252
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		145
embryo 02-04hr		77
embryo 04-06hr		154
embryo 06-08hr		135
embryo 08-10hr		182
embryo 10-12hr		118
embryo 12-14hr		56
embryo 14-16hr		24
embryo 16-18hr		16
embryo 18-20hr		14
embryo 20-22hr		7
embryo 22-24hr		9
larva L1		2
larva L2		2
larva L3 12hr old		4
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		13
white prepupae 12hr		22
white prepupae 24hr		68
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		32
adult female 05day		73
adult female 30day		72
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(145)
embryo 02-04hr		(77)
embryo 04-06hr		(154)
embryo 06-08hr		(135)
embryo 08-10hr		(182)
embryo 10-12hr		(118)
embryo 12-14hr		(56)
embryo 14-16hr		24
embryo 16-18hr		16
embryo 18-20hr		14
embryo 20-22hr		7
embryo 22-24hr		9
larva L1		2
larva L2		2
larva L3 12hr old		4
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		13
white prepupae 12hr		22
white prepupae 24hr		(68)
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		(32)
adult female 05day		(73)
adult female 30day		(72)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		(145)
embryo 02-04hr		77
embryo 04-06hr		(154)
embryo 06-08hr		(135)
embryo 08-10hr		(182)
embryo 10-12hr		(118)
embryo 12-14hr		56
embryo 14-16hr		24
embryo 16-18hr		16
embryo 18-20hr		14
embryo 20-22hr		7
embryo 22-24hr		9
larva L1		2
larva L2		2
larva L3 12hr old		4
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		13
white prepupae 12hr		22
white prepupae 24hr		68
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		32
adult female 05day		73
adult female 30day		72
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		145
embryo 02-04hr		77
embryo 04-06hr		154
embryo 06-08hr		135
embryo 08-10hr		182
embryo 10-12hr		118
embryo 12-14hr		56
embryo 14-16hr		24
embryo 16-18hr		16
embryo 18-20hr		14
embryo 20-22hr		7
embryo 22-24hr		9
larva L1		2
larva L2		2
larva L3 12hr old		4
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		13
white prepupae 12hr		22
white prepupae 24hr		68
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		32
adult female 05day		73
adult female 30day		72
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		145
embryo 02-04hr		77
embryo 04-06hr		154
embryo 06-08hr		135
embryo 08-10hr		182
embryo 10-12hr		118
embryo 12-14hr		56
embryo 14-16hr		24
embryo 16-18hr		16
embryo 18-20hr		14
embryo 20-22hr		7
embryo 22-24hr		9
larva L1		2
larva L2		2
larva L3 12hr old		4
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		13
white prepupae 12hr		22
white prepupae 24hr		68
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		32
adult female 05day		73
adult female 30day		72
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(145)
embryo 02-04hr		(77)
embryo 04-06hr		(154)
embryo 06-08hr		(135)
embryo 08-10hr		(182)
embryo 10-12hr		(118)
embryo 12-14hr		(56)
embryo 14-16hr		24
embryo 16-18hr		16
embryo 18-20hr		14
embryo 20-22hr		7
embryo 22-24hr		9
larva L1		2
larva L2		2
larva L3 12hr old		4
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		13
white prepupae 12hr		22
white prepupae 24hr		(68)
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		32
adult female 05day		(73)
adult female 30day		(72)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		(145)
embryo 02-04hr		77
embryo 04-06hr		(154)
embryo 06-08hr		135
embryo 08-10hr		(182)
embryo 10-12hr		118
embryo 12-14hr		56
embryo 14-16hr		24
embryo 16-18hr		16
embryo 18-20hr		14
embryo 20-22hr		7
embryo 22-24hr		9
larva L1		2
larva L2		2
larva L3 12hr old		4
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		13
white prepupae 12hr		22
white prepupae 24hr		68
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		32
adult female 05day		73
adult female 30day		72
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		145
embryo 02-04hr		77
embryo 04-06hr		154
embryo 06-08hr		135
embryo 08-10hr		182
embryo 10-12hr		118
embryo 12-14hr		56
embryo 14-16hr		24
embryo 16-18hr		16
embryo 18-20hr		14
embryo 20-22hr		7
embryo 22-24hr		9
larva L1		2
larva L2		2
larva L3 12hr old		4
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		13
white prepupae 12hr		22
white prepupae 24hr		68
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		4
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		32
adult female 05day		73
adult female 30day		72
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		72
Schneider line 2 Sg4		321
embryonic 1182-4H		95
embryonic GM2		50
embryonic Kc167		17
embryonic S1		25
embryonic S3		222
leg disc CME L1		216
wing disc CME-W2		97
wing disc ML-DmD8		197
wing disc ML-DmD9		129
wing disc ML-DmD16-c3		423
wing disc ML-DmD21		39
wing disc ML-DmD32		60
haltere disc ML-DmD17-c3		75
eye-antennal disc ML-DmD11		97
antennal disc ML-DmD20-c5		203
mixed discs ML-DmD4-c1		125
CNS ML-DmBG1-c1		187
CNS ML-DmBG2-c2		132
tumorous blood cells mbn2		46
ovary fGS/OSS		40
ovary OSC		28
ovary OSS		18
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(72)
Schneider line 2 Sg4		(321)
embryonic 1182-4H		(95)
embryonic GM2		(50)
embryonic Kc167		17
embryonic S1		25
embryonic S3		(222)
leg disc CME L1		(216)
wing disc CME-W2		(97)
wing disc ML-DmD8		(197)
wing disc ML-DmD9		(129)
wing disc ML-DmD16-c3		(423)
wing disc ML-DmD21		(39)
wing disc ML-DmD32		(60)
haltere disc ML-DmD17-c3		(75)
eye-antennal disc ML-DmD11		(97)
antennal disc ML-DmD20-c5		(203)
mixed discs ML-DmD4-c1		(125)
CNS ML-DmBG1-c1		(187)
CNS ML-DmBG2-c2		(132)
tumorous blood cells mbn2		(46)
ovary fGS/OSS		(40)
ovary OSC		(28)
ovary OSS		18
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		72
Schneider line 2 Sg4		(321)
embryonic 1182-4H		95
embryonic GM2		50
embryonic Kc167		17
embryonic S1		25
embryonic S3		(222)
leg disc CME L1		(216)
wing disc CME-W2		97
wing disc ML-DmD8		(197)
wing disc ML-DmD9		(129)
wing disc ML-DmD16-c3		(423)
wing disc ML-DmD21		39
wing disc ML-DmD32		60
haltere disc ML-DmD17-c3		75
eye-antennal disc ML-DmD11		97
antennal disc ML-DmD20-c5		(203)
mixed discs ML-DmD4-c1		(125)
CNS ML-DmBG1-c1		(187)
CNS ML-DmBG2-c2		(132)
tumorous blood cells mbn2		46
ovary fGS/OSS		40
ovary OSC		28
ovary OSS		18
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		72
Schneider line 2 Sg4		321
embryonic 1182-4H		95
embryonic GM2		50
embryonic Kc167		17
embryonic S1		25
embryonic S3		222
leg disc CME L1		216
wing disc CME-W2		97
wing disc ML-DmD8		197
wing disc ML-DmD9		129
wing disc ML-DmD16-c3		423
wing disc ML-DmD21		39
wing disc ML-DmD32		60
haltere disc ML-DmD17-c3		75
eye-antennal disc ML-DmD11		97
antennal disc ML-DmD20-c5		203
mixed discs ML-DmD4-c1		125
CNS ML-DmBG1-c1		187
CNS ML-DmBG2-c2		132
tumorous blood cells mbn2		46
ovary fGS/OSS		40
ovary OSC		28
ovary OSS		18
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		72
Schneider line 2 Sg4		321
embryonic 1182-4H		95
embryonic GM2		50
embryonic Kc167		17
embryonic S1		25
embryonic S3		222
leg disc CME L1		216
wing disc CME-W2		97
wing disc ML-DmD8		197
wing disc ML-DmD9		129
wing disc ML-DmD16-c3		423
wing disc ML-DmD21		39
wing disc ML-DmD32		60
haltere disc ML-DmD17-c3		75
eye-antennal disc ML-DmD11		97
antennal disc ML-DmD20-c5		203
mixed discs ML-DmD4-c1		125
CNS ML-DmBG1-c1		187
CNS ML-DmBG2-c2		132
tumorous blood cells mbn2		46
ovary fGS/OSS		40
ovary OSC		28
ovary OSS		18
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(72)
Schneider line 2 Sg4		(321)
embryonic 1182-4H		(95)
embryonic GM2		(50)
embryonic Kc167		17
embryonic S1		25
embryonic S3		(222)
leg disc CME L1		(216)
wing disc CME-W2		(97)
wing disc ML-DmD8		(197)
wing disc ML-DmD9		(129)
wing disc ML-DmD16-c3		(423)
wing disc ML-DmD21		(39)
wing disc ML-DmD32		(60)
haltere disc ML-DmD17-c3		(75)
eye-antennal disc ML-DmD11		(97)
antennal disc ML-DmD20-c5		(203)
mixed discs ML-DmD4-c1		(125)
CNS ML-DmBG1-c1		(187)
CNS ML-DmBG2-c2		(132)
tumorous blood cells mbn2		(46)
ovary fGS/OSS		(40)
ovary OSC		28
ovary OSS		18
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		72
Schneider line 2 Sg4		(321)
embryonic 1182-4H		95
embryonic GM2		50
embryonic Kc167		17
embryonic S1		25
embryonic S3		(222)
leg disc CME L1		(216)
wing disc CME-W2		97
wing disc ML-DmD8		(197)
wing disc ML-DmD9		129
wing disc ML-DmD16-c3		(423)
wing disc ML-DmD21		39
wing disc ML-DmD32		60
haltere disc ML-DmD17-c3		75
eye-antennal disc ML-DmD11		97
antennal disc ML-DmD20-c5		(203)
mixed discs ML-DmD4-c1		125
CNS ML-DmBG1-c1		(187)
CNS ML-DmBG2-c2		132
tumorous blood cells mbn2		46
ovary fGS/OSS		40
ovary OSC		28
ovary OSS		18
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		72
Schneider line 2 Sg4		321
embryonic 1182-4H		95
embryonic GM2		50
embryonic Kc167		17
embryonic S1		25
embryonic S3		222
leg disc CME L1		216
wing disc CME-W2		97
wing disc ML-DmD8		197
wing disc ML-DmD9		129
wing disc ML-DmD16-c3		423
wing disc ML-DmD21		39
wing disc ML-DmD32		60
haltere disc ML-DmD17-c3		75
eye-antennal disc ML-DmD11		97
antennal disc ML-DmD20-c5		203
mixed discs ML-DmD4-c1		125
CNS ML-DmBG1-c1		187
CNS ML-DmBG2-c2		132
tumorous blood cells mbn2		46
ovary fGS/OSS		40
ovary OSC		28
ovary OSS		18
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		59
cold shock, 4-day adult		33
heat shock, 4-day adult		50
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		64
Cadmium 100 mM 48 hrs, 4-day adult		207
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		155
Zinc 5 mM 12 hrs, larvae L3		13
Zinc 4.5 mM 48 hrs, 4-day adult		31
Ethanol 2.5% 3 hrs, larvae L3		130
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		187
Caffeine 25 mg/ml 48 hrs, 4-day adult		61
Paraquat 5 mM 48 hrs, 4-day adult		108
Paraquat 10 mM 48 hrs, 4-day adult		79
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(59)
cold shock, 4-day adult		(33)
heat shock, 4-day adult		(50)
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		(64)
Cadmium 100 mM 48 hrs, 4-day adult		(207)
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		(155)
Zinc 5 mM 12 hrs, larvae L3		13
Zinc 4.5 mM 48 hrs, 4-day adult		(31)
Ethanol 2.5% 3 hrs, larvae L3		(130)
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(187)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(61)
Paraquat 5 mM 48 hrs, 4-day adult		(108)
Paraquat 10 mM 48 hrs, 4-day adult		(79)
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		59
cold shock, 4-day adult		33
heat shock, 4-day adult		50
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		64
Cadmium 100 mM 48 hrs, 4-day adult		(207)
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		(155)
Zinc 5 mM 12 hrs, larvae L3		13
Zinc 4.5 mM 48 hrs, 4-day adult		31
Ethanol 2.5% 3 hrs, larvae L3		(130)
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(187)
Caffeine 25 mg/ml 48 hrs, 4-day adult		61
Paraquat 5 mM 48 hrs, 4-day adult		(108)
Paraquat 10 mM 48 hrs, 4-day adult		79
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		59
cold shock, 4-day adult		33
heat shock, 4-day adult		50
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		64
Cadmium 100 mM 48 hrs, 4-day adult		207
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		155
Zinc 5 mM 12 hrs, larvae L3		13
Zinc 4.5 mM 48 hrs, 4-day adult		31
Ethanol 2.5% 3 hrs, larvae L3		130
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		187
Caffeine 25 mg/ml 48 hrs, 4-day adult		61
Paraquat 5 mM 48 hrs, 4-day adult		108
Paraquat 10 mM 48 hrs, 4-day adult		79
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		59
cold shock, 4-day adult		33
heat shock, 4-day adult		50
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		64
Cadmium 100 mM 48 hrs, 4-day adult		207
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		155
Zinc 5 mM 12 hrs, larvae L3		13
Zinc 4.5 mM 48 hrs, 4-day adult		31
Ethanol 2.5% 3 hrs, larvae L3		130
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		187
Caffeine 25 mg/ml 48 hrs, 4-day adult		61
Paraquat 5 mM 48 hrs, 4-day adult		108
Paraquat 10 mM 48 hrs, 4-day adult		79
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(59)
cold shock, 4-day adult		(33)
heat shock, 4-day adult		(50)
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		(64)
Cadmium 100 mM 48 hrs, 4-day adult		(207)
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		(155)
Zinc 5 mM 12 hrs, larvae L3		13
Zinc 4.5 mM 48 hrs, 4-day adult		31
Ethanol 2.5% 3 hrs, larvae L3		(130)
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(187)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(61)
Paraquat 5 mM 48 hrs, 4-day adult		(108)
Paraquat 10 mM 48 hrs, 4-day adult		(79)
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		59
cold shock, 4-day adult		33
heat shock, 4-day adult		50
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		64
Cadmium 100 mM 48 hrs, 4-day adult		(207)
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		(155)
Zinc 5 mM 12 hrs, larvae L3		13
Zinc 4.5 mM 48 hrs, 4-day adult		31
Ethanol 2.5% 3 hrs, larvae L3		130
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(187)
Caffeine 25 mg/ml 48 hrs, 4-day adult		61
Paraquat 5 mM 48 hrs, 4-day adult		108
Paraquat 10 mM 48 hrs, 4-day adult		79
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		59
cold shock, 4-day adult		33
heat shock, 4-day adult		50
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		10
Cadmium 50 mM 48 hrs, 4-day adult		64
Cadmium 100 mM 48 hrs, 4-day adult		207
Copper 0.5 mM 12 hrs, larvae L3		11
Copper 15 mM 48 hrs, 4-day adult		155
Zinc 5 mM 12 hrs, larvae L3		13
Zinc 4.5 mM 48 hrs, 4-day adult		31
Ethanol 2.5% 3 hrs, larvae L3		130
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		187
Caffeine 25 mg/ml 48 hrs, 4-day adult		61
Paraquat 5 mM 48 hrs, 4-day adult		108
Paraquat 10 mM 48 hrs, 4-day adult		79
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

LD47579

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0003525

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG1395-RA

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for stg

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | larval stage | recessive

lethal | pharate adult stage | recessive

stg^knot

lethal | pupal stage | recessive

stg^S041621

partially lethal - majority die, with Scer\GAL4^29BD

stg^GS3120

partially lethal - majority die, with Scer\GAL4^c355

stg^GS3120

partially lethal - majority die, with Scer\GAL4^sca-4512

stg^GS-M1565

viable

viable, with Scer\GAL4^pnr-MD237

stg^GD8177

viable, with Scer\GAL4^sca-4512

Sterility

Allele

female fertile

female sterile

fertile

stg^EY12388

male sterile

stg^hs.PE2

Other Phenotypes

Allele

cell shape defective, with Scer\GAL4^Act5C.PI

stg^Scer\UAS.cNa

decreased cell size, with Scer\GAL4^{bam.T:Hsim\VP16}

stg^Scer\UAS.cNa

decreased cell size | somatic clone, with Scer\GAL4^Act5C.PP

stg^Scer\UAS.cNa

increased cell number, with Scer\GAL4^{bam.T:Hsim\VP16}

stg^Scer\UAS.cNa

increased cell size | embryonic stage | recessive

stg^AR2

increased cell size | somatic clone

mitotic cell cycle defective

mitotic cell cycle defective | embryonic stage | recessive

stg^AR2

mitotic cell cycle defective | recessive

mitotic cell cycle defective | somatic clone, with Scer\GAL4^Act5C.PP

stg^Scer\UAS.cNa

visible, with Scer\GAL4^29BD

stg^GS3120

visible, with Scer\GAL4^c355

stg^GS3120

visible, with Scer\GAL4^pnr-MD237

stg^GD8177

visible, with Scer\GAL4^sca-537.4

visible | recessive

wild-type

wild-type, with Scer\GAL4^dpp.blk1

stg^GS3120

wild-type, with Scer\GAL4^hs.2sev

stg^GS3120

Phenotype manifest in

Allele

aCC neuron | precursor

stg⁶

cardioblast

stg²

cell cycle, with Scer\GAL4^Act5C.PP

stg^Scer\UAS.cNa

centriole

stg²

chaeta, with Scer\GAL4^pnr-MD237

stg^GD8177

chordotonal organ

stg²

crystal cell

crystal cell | ectopic (with Df(2L)200)

stg²

cycle 14 embryo

stg²

cystocyte, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

denticle belt

embryo

stg²

embryonic/first instar larval cuticle

stg⁰¹²³⁵

embryonic/larval brain

stg^knot

embryonic neuroblast

stg²

external sensory organ

stg²

eye

eye-antennal disc

stg^hs.PE2

eye disc

stg^hwy

eye photoreceptor cell

follicle cell | somatic clone

stg²

ganglion mother cell

stg^hs.PE2

ganglion mother cell GMC4-2a

stg⁶

germline cyst

stg^knot

germline cyst, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

glial cell & eye disc | ectopic, with Scer\GAL4^bi-omb-Gal4

stg^Scer\UAS.cNa

haltere

imaginal disc

stg²

larval hindgut & ectoderm

stg^9M53

longitudinal connective, with Scer\GAL4^sca-4512

stg^GS-M1565

macrochaeta

macrochaeta, with Scer\GAL4^c355

stg^GS3120

macrochaeta, with Scer\GAL4^klu-G410

stg^Scer\UAS.cHa

macrochaeta, with Scer\GAL4^sca-537.4

stg^Scer\UAS.cHa

macrochaeta, with Scer\GAL4^sca-4512

stg^GS-M1565

macrochaeta & adult head

macrochaeta & adult thorax

macrochaeta & scutellum

macrochaeta & wing

macrochaeta | somatic clone, with Scer\GAL4^αTub84B.PP

stg^Scer\UAS.cHa

microchaeta, with Scer\GAL4^klu-G410

stg^Scer\UAS.cHa

microchaeta, with Scer\GAL4^sca-537.4

stg^Scer\UAS.cHa

microchaeta | somatic clone, with Scer\GAL4^αTub84B.PP

stg^Scer\UAS.cHa

mitotic cell cycle

mitotic cell cycle & eye disc

stg^hs.PE3

mitotic cell cycle | ectopic

stg^hs.PE2

mitotic cell cycle | embryonic stage

stg^unspecified

mitotic cell cycle | somatic clone, with Scer\GAL4^Act5C.PP

stg^Scer\UAS.cNa

multidendritic neuron

stg²

neuroblast NB6-4 of abdomen

stg²

neuroblast NB6-4 of thorax

stg²

neuron | embryonic stage

neuropil | embryonic stage

stg^unspecified

nuclear chromosome

nuclear membrane

ommatidium

oocyte, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

stg^{Scer\UAS.P\T.cMa}

pCC neuron

stg⁶

pCC neuron | precursor

stg⁶

pericardial cell

stg²

photoreceptor cell | ectopic

stg^hwy

photoreceptor cell R2 | ectopic

stg^hwy

photoreceptor cell R5 | ectopic

stg^hwy

plasmatocyte

stg⁰¹²³⁵

presumptive embryonic/larval peripheral nervous system

stg^S096705b

RP2sib neuron

stg⁶

scutellar bristle

scutum & macrochaeta

stg^hs.PE2

sensory mother cell, with Scer\GAL4^klu-G410

stg^Scer\UAS.cHa

socket

stg^unspecified

spermatogonial cyst, with Scer\GAL4^{bam.T:Hsim\VP16}

stg^Scer\UAS.cNa

spindle

thecogen cell | ectopic, with Scer\GAL4^sca-537.4

stg^EP3261

tormogen cell | ectopic, with Scer\GAL4^sca-537.4

trichogen cell, with Scer\GAL4^pnr-MD237

stg^GD8177

trichogen cell, with Scer\GAL4^sca-537.4

stg^EP3261

trichogen cell | ectopic, with Scer\GAL4^sca-537.4

ventral furrow, with Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, stg^AR2

stg^Scer\UAS.cNa

ventral furrow, with Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, stg^Scer\UAS.cNa

stg^AR2

ventral nerve cord commissure, with Scer\GAL4^sca-4512

wing

wing, with Scer\GAL4^29BD

stg^GS3120

wing cell

stg^hs.PE3

wing disc

Classical Alleles ( 108 )

For All Classical Alleles Show

Allele of stg	Class	Mutagen	Stocks	Known lesion
stg⁴	amorphic allele - genetic evidence	ethyl methanesulfonate	2	Yes
stg^01453b		P-element activity	1	--
stg⁰¹			1	Yes
stg^04614b			1	--
stg^5-HA-1288			1	--
stg^5-HA-1571			1	--
stg^c05524		piggyBac transposase	1	--
stg^c05571		piggyBac transposase	1	--
stg^CB-5046-3			1	--
stg^CB-6762-3			1	--
stg^d03102		P-element activity	1	--
stg^d04407		P-element activity	1	--
stg^e03714			1	--
stg^EY12388		P-element activity	1	--
stg^GS3120		Delta2-3	1	Yes
stg^KG01444b		P-element activity	1	--
stg^NP4448			1	--
stg^NP5327			1	--
stg²	loss of function allele, amorphic allele - genetic evidence	ethyl methanesulfonate	0	Yes
stg^AR2	loss of function allele, amorphic allele - genetic evidence	P-element activity	0	Yes
stg⁰¹²³⁵		P-element activity	0	--
stg⁰²⁴⁹⁹		P-element activity	0	--
stg⁰⁵⁴⁷³		P-element activity	0	--
stg¹⁰		ethyl methanesulfonate	0	--
stg¹¹	loss of function allele	P-element activity PM hybrid dysgenesis	0	Yes
stg¹²	loss of function allele	PM hybrid dysgenesis P-element activity	0	Yes
stg¹³		P-element activity	0	Yes
stg¹⁴		P-element activity	0	Yes
stg^14R2		Delta2-3	0	Yes
stg^14R5		Delta2-3	0	Yes
stg¹	hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	--
stg³	loss of function allele	ethyl methanesulfonate	0	--
stg^3D1		P-element activity	0	--
stg⁵	loss of function allele	ethyl methanesulfonate	0	--
stg⁶	loss of function allele, hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	--
stg⁷⁶			0	--
stg⁷	hypermorphic allele - genetic evidence, hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	--
stg⁸	hypermorphic allele - genetic evidence, hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	--
stg⁹		ethyl methanesulfonate	0	--
stg^9M53			0	--
stg^AA53		P-element activity	0	--
stg^AR1		P-element activity	0	--
stg^AR3		P-element activity	0	--
stg^AR4	loss of function allele	P-element activity	0	--
stg^AR5	loss of function allele	P-element activity	0	Yes
stg^AR6		P-element activity	0	--
stg^AR7		P-element activity	0	--
stg^AR8		P-element activity	0	--
stg^BG02313		P-element activity	0	Yes
stg^CB-6871-3			0	--
stg^CB03726		P-element activity	0	Yes
stg^d00810		P-element activity	0	--
stg^d02477		P-element activity	0	--
stg^d05881		P-element activity	0	--
stg^D115		X ray ethyl methanesulfonate	0	--
stg^dd1		ethyl methanesulfonate	0	--
stg^dd2		ethyl methanesulfonate	0	--
stg^dd3		ethyl methanesulfonate	0	--
stg^DD937		X ray ethyl methanesulfonate	0	--
stg^DG05404		P-element activity	0	--
stg^Dr-1			0	Yes
stg^Dr-FA30			0	Yes
stg^Dr-L2			0	Yes
stg^Dr-Mio			0	--
stg^Dr-mr21			0	--
stg^DY		P-element activity	0	--
stg^eAS7a		ethyl methanesulfonate	0	--
stg^EP1213		P-element activity	0	Yes
stg^EP3040		P-element activity	0	--
stg^EP3261		P-element activity	0	Yes
stg^EP3426		P-element activity	0	Yes
stg^EP3432		P-element activity	0	Yes
stg^EPg30417		P-element activity	0	Yes
stg^GS-M1565		P-element activity	0	Yes
stg^HLE			0	Yes
stg^hwy		spontaneous	0	Yes
stg^j10B9		P-element activity	0	--
stg^j1D3		P-element activity	0	--
stg^j1E3		P-element activity	0	--
stg^j3A1a		P-element activity	0	--
stg^j3D1		P-element activity	0	--
stg^knot		ethyl methanesulfonate	0	--
stg^MI08204			0	--
stg^RXT13			0	--
stg^ry129		P-element activity	0	--
stg^ry80		P-element activity	0	--
stg^S(rux)3A		ethyl methanesulfonate X ray	0	--
stg^S022406		Delta2-3 P-element activity	0	--
stg^S024503		Delta2-3 P-element activity	0	--
stg^S024532		P-element activity	0	Yes
stg^S041621		P-element activity	0	--
stg^S043922		Delta2-3 P-element activity	0	--
stg^S073013		Delta2-3 P-element activity	0	--
stg^S089605a		Delta2-3 P-element activity	0	--
stg^S096705b		Delta2-3 P-element activity	0	--
stg^S098006		Delta2-3 P-element activity	0	--
stg^S106006a		Delta2-3 P-element activity	0	--
stg^S108306b		Delta2-3 P-element activity	0	--
stg^S108310b		P-element activity	0	--
stg^S108313b		Delta2-3 P-element activity	0	--
stg^S108908		Delta2-3 P-element activity	0	--
stg^S114302b		Delta2-3 P-element activity	0	--
stg^s2213		P-element activity	0	--
stg^sCG4		X ray	0	--
stg^sM3		ethyl methanesulfonate	0	--
stg^unspecified			0	--
stg^X1		X ray	0	--
stg^YD0685		piggyBac transposase	0	--

Alleles Carried on Transgenic Constructs ( 29 )

For All Alleles Carried on Transgenic Constructs Show

Allele of stg	Mutagen	Stocks	Known lesion
stg^Scer\UAS.cNa	in vitro construct - regulatory fusion	4	Yes
stg^GD8177	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
stg^GL00513	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
stg^HMS00146	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
stg^JF03235	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
stg^{9A.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis	0	Yes
stg^a.cPa	in vitro construct - RNAi	0	Yes
stg^BCD	in vitro construct - genomic fragment	0	Yes
stg^BCDE	in vitro construct - genomic fragment	0	Yes
stg^cBa	in vitro construct	0	--
stg^CD	in vitro construct - genomic fragment	0	Yes
stg^cPa	in vitro construct - RNAi	0	Yes
stg^{CR-SK.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis	0	Yes
stg^cWa	in vitro construct	0	Yes
stg^{fs(1)K10.Kstem5'3'}	in vitro construct - site directed mutagenesis	0	Yes
stg^{fs(1)K10.Kstem5'}	in vitro construct - site directed mutagenesis	0	Yes
stg^fs(1)K10.TLS	in vitro construct	0	Yes
stg^hs.PE2	in vitro construct - regulatory fusion	0	Yes
stg^hs.PE3	in vitro construct - regulatory fusion	0	Yes
stg^{MtnA.T:Ivir\HA1}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
stg^NIG.1395R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
stg^Scer\UAS.cHa	in vitro construct - regulatory fusion	0	--
stg^Scer\UAS.cLa	in vitro construct - regulatory fusion	0	Yes
stg^{Scer\UAS.P\T.cMa}	in vitro construct - regulatory fusion	0	Yes
stg^{Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
stg^{Scer\UAS.T:Zzzz\Dronpa}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
stg^t31.6	in vitro construct - genomic fragment	0	Yes
stg^t6.0	in vitro construct - genomic fragment	0	Yes
stg^twi.PS	in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(3R)01215

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(3R)3450

(Szeged Stock Center, 1998-, Verheyen et al., 1996, Hayashi et al., 1997)

Df(3R)B219

(Muller et al., 1999)

Df(3R)BSC500

(Christensen et al., 2008.4.15)

Df(3R)BSC501

(Christensen et al., 2008.4.15)

Df(3R)BSC846

(Christensen et al., 2009.7.22)

Df(3R)Dr-RXT13

(Tearle et al., 1994)

Df(3R)ED6310

(Ryder and Roote, 2004.11.10)

Df(3R)ED6316

(Ryder and Roote, 2004.11.10)

Df(3R)Exel6212

(Ryder, 2004.4.26)

Df(3R)J55

(Jurgens et al., 1984)

Df(3R)Ptp99A^R3

(Mozer and Easwarachandran, 1999)

Df(3R)R61

(Mozer, 2001)

Df(3R)stg^AR2

(Edgar et al., 1994, Edgar and Datar, 1996, White-Cooper et al., 1996)

Df(3R)stg^AR5

(Edgar and Datar, 1996)

Ts(YSt;3Lt)G75

(Jurgens et al., 1984)

Ts(YSt;3Lt)R128

(Jurgens et al., 1984)

Not disrupted in

Df(3R)3450

(Spradling et al., 1999, BDGP Project Members, 1994-1999, Salzberg et al., 1997)

Df(3R)KE

(Mozer and Easwarachandran, 1999)

Df(3R)sbd45

(Salzberg et al., 1997)

Duplicated in

Dp(3;Y)B219

(Grosshans and Wieschaus, 2000)

Dp(3;Y)J55

(Grosshans and Wieschaus, 2000)

Ts(YSt;3Rt)B226

(Jurgens and Weigel, 1988)

Ts(YSt;3Rt)J116

(Jurgens and Weigel, 1988)

Not duplicated in

Dp(3;1)Dr-RXT11

(Jurgens and Weigel, 1988)

Dp(3;Y)B204

(Grosshans and Wieschaus, 2000)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

reporter construct

P{stg-lacZ.HZ}

P{stg-lacZ.HZ-B8.2}

P{stg-lacZ.HZ-BEa1.2}

P{stg-lacZ.HZ-SpEa2.3}

heat-shock construct

UAS construct

P{UAS-stg.CR-SK.eGFP}

characterization construct

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

insertion of enhancer trap

miscellaneous insertions

insertion of enhancer trap binary system

P{GawB}stg^NP4448

P{GawB}stg^NP5327

Gene Ontology: Function, Process & Cellular Component ( 26 unique terms )

Terms Based on Experimental Evidence ( 19 terms )

Molecular Function

CV term

Evidence

References

protein tyrosine phosphatase activity

inferred from direct assay

(Dunphy and Kumagai, 1991)

Biological Process

CV term

Evidence

References

cell cycle

inferred from mutant phenotype

(Kiger et al., 2003)

cell proliferation

inferred from mutant phenotype

(Kondylis et al., 2011)

cellular process

inferred from mutant phenotype

(Boutros et al., 2004)

centriole replication

inferred from mutant phenotype

(Vidwans et al., 1999)

G2/M transition of mitotic cell cycle

inferred from mutant phenotype

(Edgar and O'Farrell, 1990, Chen et al., 2007)

gastrulation

inferred from mutant phenotype

(Grosshans and Wieschaus, 2000)

Golgi organization

inferred from mutant phenotype

(Kondylis et al., 2011, Zacharogianni et al., 2011)

lateral inhibition

inferred from mutant phenotype

(Mummery-Widmer et al., 2009)

negative regulation of cell size

inferred from mutant phenotype

(Zacharogianni et al., 2011)

negative regulation of mitotic cell cycle

inferred from mutant phenotype

(Kondylis et al., 2011)

positive regulation of cell proliferation

inferred from mutant phenotype

(Zacharogianni et al., 2011)

positive regulation of mitotic cell cycle

inferred from mutant phenotype

(Zacharogianni et al., 2011)

positive regulation of mitotic cell cycle, embryonic

inferred from mutant phenotype

(Di Talia and Wieschaus, 2012)

protein dephosphorylation

inferred from direct assay

(Dunphy and Kumagai, 1991)

regulation of cell diameter

inferred from mutant phenotype

(Kondylis et al., 2011)

regulation of mitotic cell cycle

inferred from mutant phenotype

(Pile et al., 2002)

Cellular Component

CV term

Evidence

References

chromosome

inferred from direct assay

(Edgar et al., 1994)

nucleus

inferred from direct assay

(Edgar et al., 1994)

Terms Based on Predictions or Assertions ( 10 terms )

Molecular Function

CV term

Evidence

References

non-membrane spanning protein tyrosine phosphatase activity

non-traceable author statement

(Swiss-Prot Project Members, 1991.2.1)

protein tyrosine/serine/threonine phosphatase activity

non-traceable author statement

(Morrison et al., 2000)

Biological Process

CV term

Evidence

References

G2/M transition of mitotic cell cycle

inferred from sequence or structural similarity

(Edgar and O'Farrell, 1989)

non-traceable author statement

(Ghysen and Thomas, 2003)

traceable author statement

(Lee and Orr-Weaver, 2003)

gastrulation

non-traceable author statement

(Leptin, 1999)

mesoderm development

traceable author statement

(Leptin, 1999)

M phase of mitotic cell cycle

inferred from electronic annotation with InterPro:IPR000751

(FlyBase Curators et al., 2004-)

open tracheal system development

traceable author statement

(Zelzer and Shilo, 2000)

peripheral nervous system development

traceable author statement

(Prokopenko et al., 2000)

protein dephosphorylation

non-traceable author statement

(Morrison et al., 2000)

regulation of mitosis

non-traceable author statement

(Swiss-Prot Project Members, 1991.2.1)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

stg - CG11266

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

stg - RpL7-like

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

stg allele

Gene

References

stg¹

CycE

(Secombe et al., 1998)

stg²

arm

(Greaves et al., 1999)

stg⁴

Myb

(Okada et al., 2002)

stg⁵

CycE

(Secombe et al., 1998)

stg^AR2

CycE

(Secombe et al., 1998)

(Bianchi-Frias et al., 2004)

pUf68

(Quinn et al., 2004)

rux

(Thomas et al., 1994)

(Verheyen et al., 1996)

stg^EP3040

pnr

(Pena-Rangel et al., 2002)

stg^EP3261

pnr

(Pena-Rangel et al., 2002)

stg^EP3426

pnr

(Pena-Rangel et al., 2002)

stg^EP3432

pnr

(Pena-Rangel et al., 2002)

stg^hs.PE2

Myb

(Katzen et al., 1998)

stg^hs.PE3

Egfr

(Kylsten and Saint, 1997)

stg^RXT13

rux

(Thomas et al., 1994)

(Perrin et al., 2003)

pnt

(Baonza et al., 2002)

trbl

(Mata et al., 2000)

ttk

(Baonza et al., 2002)

stg^{Scer\UAS.P\T.cMa}

trbl

(Mata et al., 2000)

stg^sCG4

(Verheyen et al., 1996)

stg^sM3

(Verheyen et al., 1996)

stg^unspecified

CycE

(Brumby et al., 2004)

unspecified

(White-Cooper et al., 1996)

pbl

(Gregory et al., 2007)

shg

(Greaves et al., 1999)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

68333

DPiM - DPiM, Drosophila Protein interaction Map

•

FBgn0003525

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0003525

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

43466

Orthologs

OrthoDB Orthologs (33) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG60ZRHF

Dipteran inclusive ortholog search

EOG698V88

Insect inclusive ortholog search

EOG6254Q8

Arthropod inclusive ortholog search

EOG6V9S75

Metazoa inclusive ortholog search

EOG63C0GC

Orthologs in Drosophila Species (EOG60ZRHF)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA12648

Drosophila persimilis

Dper\GL13601

Drosophila willistoni

Dwil\GK11032

Drosophila virilis

Dvir\GJ14225

Drosophila mojavensis

Dmoj\GI22346

Drosophila grimshawi

Dgri\GH14002

Orthologs in non-Drosophila Dipterans (EOG698V88)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL014408

Anopheles gambiae

Malaria mosquito

Agam\AGAP003301

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ001662

Orthologs in non-Dipteran Insects (EOG6254Q8)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\LOC726510

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG006799

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH19182

Atta cephalotes

Leafcutter ant

Acep\ACEP27313

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO20349

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL22995

Linepithema humile

Argentine ant

Lhum\LH20520

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB21174

Solenopsis invicta

Red fire ant

Sinv\SINV11316

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000305

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005531

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005336

Pediculus humanus

Human body louse

Phum\PHUM420140

Tribolium castaneum

Red flour beetle

Tcas\twe

Orthologs in non-Insect Arthropods (EOG6V9S75)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_44847

Ixodes scapularis

Deer tick

Isca\ISCW005282

Orthologs in non-Arthropod Metazoa (EOG63C0GC)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\cdc-25.2

Danio rerio

Zebrafish

Drer\cdc25d

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA12648

Drosophila persimilis

Dper\GL13601

Drosophila willistoni

Dwil\GK11032

Drosophila virilis

Dvir\GJ14225

Drosophila mojavensis

Dmoj\GI22346

Drosophila grimshawi

Dgri\GH14002

Stocks & Reagents

Stocks Listed in FlyBase ( 27 )

Bloomington

4778

w¹¹¹⁸; P{UAS-stg.N}4

4777

w¹¹¹⁸; P{UAS-stg.N}16

107873

107872

w¹¹¹⁸; P{UAS-stg.N}16

123428

w¹¹¹⁸; P{RS3}stg^CB-5046-3

124210

w¹¹¹⁸; P{RS3}stg^CB-6762-3

VDRC

v17760

w¹¹¹⁸; P{GD8177}v17760/TM3

More stocks available...

Genomic Clones ( 1 )

BACR22E21

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 80 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD47579

Other clones

DPiM_LD47579

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0003525

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

43466

Antibody Information

polyclonal

(Di Talia et al., 2013, Inaba et al., 2011)

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: stg CG1395

(Liang, 1999.11)

Source for database merge of

Source for merge of: stg EP1213

(Bidet et al., 2003)

Additional comments

P{EP} insertion in "EP1213^EP1213" is approximately 1.5kb upstream of the stg transcription unit. However, it is not clear whether stg or a gene other than stg is affected in the P{EP}stg^EP1213 insertion line.

(Abdelilah-Seyfried et al., 2000)

"l(3)06743⁰⁶⁷⁴³" may be a leaky allele of "stg".

(Spradling, 1999.12.9)

"Dr" mutants are always double hits in two genes, one of which is "stg".

(Mozer, 1998.3.17)

Interactions between "Dr" revertant alleles and "stg" demonstrate they are separate loci.

(Tearle et al., 1994)

Can rescue allele 22 of Spom\cdc25, but not a deletion of the gene.

(Alphey et al., 1992)

Other Comments

Expression is enriched in embryonic gonads.

(Shigenobu et al., 2006)

dsRNA made from templates generated with primers directed against stg that is transfected into S2 treated with Listeria monocytogenes reveals stg to be involved in Listeria monocytogenes intracellular growth, with increased intracellular growth observed in stg-treated cells.

(Cheng et al., 2005)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R⁺ cells: cell size is increased, microtubules are uniform or disorganised, cell shape is irregular, and cell number is decreased indicative of a failure in cell cycle progression through G1 to S and G2 to M stages.

(Kiger et al., 2003)

The joint action of two RNA degradation pathways (a maternally encoded and a zygotic pathway) controls maternal transcript degradation and its timing in the early embryo. stg transcripts (relatively high in abundance) require the action of both pathways in order to be eliminated prior to the midblastula transition.

(Bashirullah et al., 1999)

Many modular elements with separable activities, spread over more than 30kb, control stg transcription in the embryo and imaginal discs.

(Lehman et al., 1999)

The Dr mutants are cis-regulatory alleles of stg, as well as alleles of Dr.

(Mozer and Easwarachandran, 1999)

stg is required for completion of daughter centriole assembly in embryos.

(Vidwans et al., 1999)

numb functions downstream of cell division genes (CycA, Rca1 and stg) and progression through the cell cycle is required for asymmetric localisation of numb and thus N mediated specification of the sib fate in the RP2/sib division.

(Wai et al., 1999)

The loss of CycA, Rca1 or stg leads to a block in the division of GMC-1, however this GMC-1 adopts a RP2 identity.

(Wai et al., 1999)

Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.

(Harvie et al., 1998)

The wg product induces G2 arrest in two subdomains of the developing wing margin by inducing ac and sc, which down-regulate stg.

(Johnston and Edgar, 1998)

Identification: Enhancer trap screen designed to discover genes involved in the cellular aspects of defense mechanisms, as well as in melanotic tumor formation processes linked to blood cell disregulation.

(Braun et al., 1997)

stg regulation is a critical part of the control of early entry into mitosis in some, but not all, G₂-arrested imaginal cells, identifying an additional mechanisms by which cellular proliferation is controlled in the growing adult tissues. stg is essential for the generation of the adult cuticle.

(Kylsten and Saint, 1997)

stg and twe complement each other in the germ-line and early embryo. stg is required for neither female germ cell divisions nor embryonic cycles 1-13. Lowering the maternal dose of stg and twe can advance the maternal/zygotic transition (MZT). Increasing twe, but not stg, can postpone the MZT.

(Edgar and Datar, 1996)

Genetic analysis of the Dr locus suggests it defines a novel regulator of stg.

(Mozer, 1995)

rux acts genetically to negatively regulate stg.

(Thomas et al., 1995)

The prolonged halflife of the mus209 protein in stg mutant embryos suggests involvement of phosphorylation or dephosphorylation of some proteins in disassembly of the DNA replication enzyme complex.

(Yamaguchi et al., 1995)

The coordinate program of expression of S phase genes (DNApol-α180, mus209, RnrL and RnrS) is not disrupted in stg mutants and is therefore not a secondary consequence of cell cycle progression.

(Duronio and O'Farrell, 1994)

Degradation of maternally supplied stg causes tyrosine dephosphorylation of cdc2 to become rate limiting for mitosis beginning in cycle 14.

(Edgar et al., 1994)

Known patterning genes act locally to influence stg transcription. Complete pattern of stg transcription requires >15.3kb of cis-acting regulatory sequences. stg transcription is largely unaffected in mutant embryos arrested in G₂ of cycles 14, 15 or 16, or G₁ of cycle 17. Thus there is a regulatory hierarchy in which developmental inputs, not cell cycle inputs, control the timing of stg transcription and hence cell cycle progression. Overall orientation not stated: anon-99A? stg+ ptls+

(Edgar et al., 1994)

stg is epistatic to fzy.

(Dawson et al., 1993)

Mutants are embryonic lethal; denticle bands are reduced. First 13 nuclear divisions of zygote proceed on schedule; division 14 permanently arrested in G2 in homozygotes for strong alleles; no evidence of nuclear-envelope breakdown or chromosome condensation; division 14 severely impaired in weak alleles. Gastrulation proceeds on schedule in stg mutant embryos with but 5,000 cells. The only defect seems to be in the initiation of the first mitotic division that is under zygotic control. Clones of homozygous cells induced early don't survive; few late clones produced.

(Lindsley and Zimm, 1992)

DNA synthesis in arrested stg embryos confined to the polyploid amnioserosal cells. Markers ordinarily expressed in normal embryos with 50,000 cells also expressed in stg embryos with 5,000 cells.

(Lindsley and Zimm, 1992)

Mutations in zygotic gene stg do not interact with RpII140^wimp.

(Parkhurst and Ish-Horowicz, 1991)

The effects of an altered nucleocytoplasmic ratio on transcripts that normally undergo changes in transcript pattern in cell cycle 14 is studied. A delay in the maternal-to-zygotic transition of the mitotic control gene stg is correlated with a decrease in nuclear density and a change in the cell cycle program.

(Yasuda et al., 1991)

The normal number of cell divisions is important to achieve proper cuticular differentiation, whereas the relative timing of these divisions is less critical.

(Edgar and O'Farrell, 1990)

stg gene product activity is rate limiting for the cell cycle transition G2/M during embryonic cell cycles 14, 15 and 16.

(Edgar and O'Farrell, 1990)

Regulated expression of stg mRNA controls the timing and location of zygotically driven embryonic cell divisions.

(Edgar and O'Farrell, 1989)

stg mutants display a strong reduction in the number of denticle rows.

(Jurgens et al., 1984)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

Entrez Gene - A searchable database of RefSeq genes.

•

43466

UniProtKB/Swiss-Prot

•

P20483

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

M-phase inducer phosphatase (IPR000751)

Rhodanese-like domain (IPR001763)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

LD47579

Linkouts

BioGRID - A database of protein and genetic interactions

•

68333

DPiM - DPiM, Drosophila Protein interaction Map

•

FBgn0003525

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0003525

DRSC - Results from RNAi screens.

•

FBgn0003525

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0003525

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG1395-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0003525

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

43466

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

•

/newgene/string1.htm

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

43466

modMine - Data generated by the modENCODE project.

•

FBgn0003525

REDfly - A database of transcriptional regulatory elements.

•

FBgn0003525

Synonyms & Secondary IDs ( 46 )

Reported As

Symbol Synonym

0224/06

0245/03

0439/22

0730/13

0896/05

0967/05

0980/06

1083/13

1089/08

1143/02

5473

(Harvie et al., 1998)

anon-EST:Liang-2.21

Cdc25

(Benton and St. Johnston, 2003, Vidwans et al., 2002, Edgar, 1995, Edgar et al., 1994, Jordan et al., 2006, Kondylis et al., 2007, Zielke et al., 2008, Mitchell et al., 2008, Ninov et al., 2009, McCleland et al., 2009, Glavic et al., 2009, Kondylis et al., 2011, Hong et al., 2008, Farrell et al., 2012)

CDC25

(Bakal et al., 2008, Gawlinski et al., 2007, Davidson et al., 2009)

Cdc25^stg

(Su and O'Farrell, 1998, Su et al., 1998)

Cdc25^Stg

(de Vries et al., 2005)

cdc25^string

(Coffman, 2004, Price et al., 2002, Myster and Duronio, 2000, Di Talia and Wieschaus, 2012, Di Talia and Wieschaus, 2012)

CDC25^string

(O'Farrell, 2001)

Cdc25^String

(Vidwans and Su, 2001, Marshall, 2001, Follette and O'Farrell, 1997)

Cdc25^string

(Bissen, 1997)

Cdc25c

(Morse et al., 2009)

CG1395

(Norga et al., 2003, Morrison et al., 2000, Quinones-Coello, 2007, Quinones-Coello, 2007, Mosallanejad et al., 2010, Krejcí et al., 2009, Perkins et al., 2009, Ni et al., 2011.7.19, Wheeler et al., 2004)

clone 2.21

(Liang and Biggin, 1998)

EP1213

(Abdelilah-Seyfried et al., 2000, )

l(3)01235

(Spradling, 1999.12.9, Braun et al., 1997, Perrimon et al., 1996, )

l(3)j1D3

l(3)j1E3

l(3)j3D1

l(3)j10B9

l(3)s2213

S(rux)3A

(Dong et al., 1997)

(Omel'yanchuk et al., 2007)

stg

(Swaminathan and Pile, 2010, Gibson et al., 2006, Wells et al., 2006, Odenwald, 2005, Shigenobu et al., 2006, Mukherjee et al., 2006, Ji et al., 2012, Park et al., 2003, Sandmann et al., 2007, Zhai et al., 2012, Chen et al., 2007, Delanoue et al., 2004, Buszczak et al., 2007, Quinones-Coello, 2007, Quinones-Coello, 2007, Arancio et al., 2010, Shibutani et al., 2007, Zhang et al., 2007, Christensen et al., 2008.4.15, Christensen et al., 2008.4.15, Mesquita et al., 2010, O'Farrell and Kylsten, 2008, Di Talia and Wieschaus, 2012, Frise et al., 2010, Kankel et al., 2007, Fox et al., 2010, Chen et al., 2013, Sun and Deng, 2007, Buttitta et al., 2007, Choksi et al., 2006, da Silva and Vincent, 2007, Chang et al., 2008, Jemc and Rebay, 2007, White et al., 2007, Mitchell et al., 2008, Krejcí et al., 2009, Read et al., 2009, Andersen and Tapon, 2008, Christensen et al., 2009.7.22, Gawlinski et al., 2007, Davidson et al., 2009, Bhattacharya and Baker, 2009, Grosskortenhaus et al., 2005, Heriche et al., 2003, Insco et al., 2009, McCleland et al., 2009, Shyu et al., 2009, Gim et al., 2001, Morris et al., 2006, Insco et al., 2009, Berger et al., 2010, Lopes and Casares, 2010, Zhou et al., 2008, Rouault et al., 2010, Bernard et al., 2010, Schittenhelm et al., 2010, Nakamura et al., 2008, Loop et al., 2004, Trunova et al., 2001, Reid et al., 2012, Silicheva et al., 2010, Takahashi et al., 2012, Korenjak et al., 2012, Di Talia and Wieschaus, 2012, Japanese National Institute of Genetics, 2012.5.21, Farrell et al., 2012, Sun and Spradling, 2012, Althoff et al., 2012, Aliee et al., 2012, Marinari et al., 2012, Manning et al., 2012, Pinto et al., 2011)

Stg

(Tanaka-Matakatsu et al., 2007, Baehrecke, 2006, Calzone et al., 2007, Rimkus et al., 2008, Rafel and Milán, 2008, Glavic et al., 2009, Tanaka-Matakatsu et al., 2007, Buttitta et al., 2010)

STG

(Pile et al., 2002)

stg^cdc25

(Lanzotti et al., 2004, Johnston and Sanders, 2003)

str/cdc25

(Nabel-Rosen et al., 2005)

string

(Becam et al., 2011, Ninov et al., 2009)

String/Cdc25

(Jacobs et al., 2003)

SY3-4

(Rebay et al., 2000)

Name Synonym

Cdc25

(Glavic et al., 2009)

cdc25/string

(Lopes and Casares, 2010)

String

(Swaminathan and Pile, 2010, Thomas, 2005, Jordan et al., 2006, Baehrecke, 2006, Calzone et al., 2007, Morse et al., 2009, Kondylis et al., 2007, Rimkus et al., 2008, Rafel and Milán, 2008, Glavic et al., 2009, Gim et al., 2001, Kondylis et al., 2011, Wheeler et al., 2004, Reid et al., 2012, Hong et al., 2008, Gupta et al., 2008)

string

(Jafar-Nejad and Bellen, 2004, Su et al., 2000, Wodarz and Gonzalez, 2006, Hyun et al., 2006, Silva and Vincent, 2007, Jemc and Rebay, 2007, Gibson et al., 2006, Sanchez-Elsner et al., 2006, Sanchez-Elsner et al., 2006, Maile et al., 2004, Gregory et al., 2007, Mukherjee et al., 2006, Foglietti et al., 2006, Garcia et al., 2007, Estella and Mann, 2010, Hutterer et al., 2006, Odenwald, 2005, Mesquita et al., 2010, O'Farrell and Kylsten, 2008, Bellotto et al., 2002, Moshkin et al., 2007, Somma et al., 2008, Kankel et al., 2007, Southall and Brand, 2009, Herranz et al., 2008, Sun and Deng, 2007, Buttitta et al., 2007, Choksi et al., 2006, da Silva and Vincent, 2007, De Renzis et al., 2007, Chang et al., 2008, Jemc and Rebay, 2007, White et al., 2007, Zielke et al., 2008, Thomsen et al., 2010, Krejcí et al., 2009, Lu et al., 2009, Gawlinski et al., 2007, Davidson et al., 2009, Bhattacharya and Baker, 2009, Insco et al., 2009, Tanaka-Matakatsu et al., 2007, McCleland et al., 2009, Shyu et al., 2009, Wilkie et al., 2001, Morris et al., 2006, Sims et al., 2009, Berger et al., 2010, Rouault et al., 2010, Sung et al., 2013, Bossing et al., 2012, Nakamura et al., 2008, Omel'yanchuk et al., 2007, Trunova et al., 2001, Kaplow et al., 2007, Wheeler et al., 2004, Farrell et al., 2012)

string/cdc25

(Lopes and Casares, 2010, Baig et al., 2010)

String/Cdc25 phosphatase

(Reber et al., 2006)

Suppressor of rux 3A

(Dong et al., 1997)

Secondary FlyBase IDs

FBgn0011019
FBgn0011315
FBgn0011317
FBgn0011354
FBgn0011428
FBgn0043376

References ( 530 )

Generate a list of

All references relating to this gene ( 530 )

List References by type

Research paper ( 316 )
Supplementary material ( 15 )
Review ( 83 )
Personal communication to FlyBase ( 24 )
Abstract ( 74 )
FlyBase analysis ( 3 )
Conference report ( 5 )
Stock list ( 3 )
Curated genome annotation ( 3 )
Book ( 1 )
DNA/RNA sequence record ( 1 )
Protein sequence record ( 1 )
Website ( 1 )

Recent research papers ( 25 )

Chen et al., 2013, RNA 19(2): 208--218: BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing. [FBrf0220579]
Di Talia et al., 2013, Curr. Biol. 23(2): 127--132: Posttranslational Control of Cdc25 Degradation Terminates Drosophila's Early Cell-Cycle Program. [FBrf0220610]
Pitsouli and Perrimon, 2013, Sci. Signal. 6(263): ra12: The homeobox transcription factor cut coordinates patterning and growth during Drosophila airway remodeling. [FBrf0220848]
Sung et al., 2013, Curr. Biol. 23(2): 133--138: Number of nuclear divisions in the Drosophila blastoderm controlled by onset of zygotic transcription. [FBrf0220648]
Zhan et al., 2013, PLoS ONE 8(2): e57214: Identification of Genetic Modifiers of TDP-43 Neurotoxicity in Drosophila. [FBrf0220979]
Aliee et al., 2012, Curr. Biol. 22(11): 967--976: Physical mechanisms shaping the Drosophila dorsoventral compartment boundary. [FBrf0218592]
Althoff et al., 2012, Mol. Biol. Cell 23(12): 2275--2291: Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1. [FBrf0218626]
Bossing et al., 2012, Dev. Cell 23(2): 433--440: Disruption of Microtubule Integrity Initiates Mitosis during CNS Repair. [FBrf0219196]
Di Talia and Wieschaus, 2012, Dev. Cell 22(4): 763--774: Short-Term Integration of Cdc25 Dynamics Controls Mitotic Entry during Drosophila Gastrulation. [FBrf0218101]
Farrell et al., 2012, Genes Dev. 26(7): 714--725: Embryonic onset of late replication requires Cdc25 down-regulation. [FBrf0217935]
Ji et al., 2012, G3 (Bethesda) 2(12): 1651--1660: In Vivo Regulation of E2F1 by Polycomb Group Genes in Drosophila. [FBrf0220353]
Korenjak et al., 2012, Mol. Cell. Biol. 32(21): 4375--4387: RBF Binding to both Canonical E2F Targets and Noncanonical Targets Depends on Functional dE2F/dDP Complexes. [FBrf0219643]
Manning et al., 2012, Cell Rep. 2(4): 1002--1013: A Resource for Manipulating Gene Expression and Analyzing cis-Regulatory Modules in the Drosophila CNS. [FBrf0219785]
Marinari et al., 2012, Nature 484(7395): 542--545: Live-cell delamination counterbalances epithelial growth to limit tissue overcrowding. [FBrf0218620]
Reid et al., 2012, Front. Cell. Infect. Microbiol. 2: 24: Identification of genetic modifiers of CagA-induced epithelial disruption in Drosophila. [FBrf0219288]
Sun and Spradling, 2012, Curr. Biol. 22(10): 862--871: NR5A Nuclear Receptor Hr39 Controls Three-Cell Secretory Unit Formation in Drosophila Female Reproductive Glands. [FBrf0218434]
Takahashi et al., 2012, Evolution 66(9): 2878--2890: Deficiency screening for genomic regions with effects on environmental sensitivity of the sensory bristles of Drosophila melanogaster. [FBrf0219405]
Zhai et al., 2012, PLoS Genet. 8(3): e1002582: Antagonistic regulation of apoptosis and differentiation by the cut transcription factor represents a tumor-suppressing mechanism in Drosophila. [FBrf0217859]
Becam et al., 2011, Development 138(17): 3781--3789: Notch-mediated repression of bantam miRNA contributes to boundary formation in the Drosophila wing. [FBrf0214603]
Clark et al., 2011, Development 138(13): 2705--2715: Fibroblast growth factor signalling controls successive cell behaviours during mesoderm layer formation in Drosophila. [FBrf0213889]
Horn et al., 2011, Nat. Methods 8(4): 341--346: Mapping of signaling networks through synthetic genetic interaction analysis by RNAi. [FBrf0213352]
Inaba et al., 2011, Development 138(23): 5079--5086: String (Cdc25) regulates stem cell maintenance, proliferation and aging in Drosophila testis. [FBrf0216589]
Kondylis et al., 2011, PLoS ONE 6(2): e17173: Identification of ER Proteins Involved in the Functional Organisation of the Early Secretory Pathway in Drosophila Cells by a Targeted RNAi Screen. [FBrf0213167]
Pinto et al., 2011, EMBO J. 30(12): 2431--2444: RNA polymerase II kinetics in polo polyadenylation signal selection. [FBrf0213944]
Zacharogianni et al., 2011, EMBO J. 30(18): 3684--3700: ERK7 is a negative regulator of protein secretion in response to amino-acid starvation by modulating Sec16 membrane association. [FBrf0215221]

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Recent reviews (0)

All reviews listed in FlyBase were published before 2011

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