FlyBase Gene Report: Dmel\sub

General Information

Symbol

Dmel\sub

Species

D. melanogaster

Name

subito

Annotation Symbol

CG12298

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003545

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Summary

subito (sub) encodes a kinesin-6 homolog. Kinesin-6 proteins are known to bundle antiparallel microtubules. The product of sub is required for cytokinesis in mitosis and spindle organization and chromosome segregation in female meiosis. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

Dub, mei-1794, Double or nothing, Mklp2

Key Links

Genomic Location

Cytogenetic map

54E7-54E7

Sequence location

2R:17,745,500..17,748,078 [-]

Recombination map

2-84

RefSeq locus

NT_033778 REGION:17745500..17748078

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (25 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables gamma-tubulin complex binding

inferred from direct assay

(Romé and Ohkura, 2018)

enables microtubule motor activity

inferred from mutant phenotype

(assigned by UniProt )

(Giunta et al., 2002)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables ATP hydrolysis activity

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(assigned by GO_Central )

(Gaudet et al., 2011)

enables microtubule binding

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(assigned by GO_Central )

(Gaudet et al., 2011)

enables microtubule motor activity

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (12 terms)

Terms Based on Experimental Evidence (11 terms)

CV Term

Evidence

References

involved_in embryonic cleavage

inferred from mutant phenotype

(assigned by UniProt )

(Giunta et al., 2002)

involved_in female meiosis chromosome segregation

inferred from mutant phenotype

(Giunta et al., 2002)

inferred from genetic interaction with FLYBASE:ncd; FB:FBgn0002924

(Giunta et al., 2002)

inferred from genetic interaction with FLYBASE:nod; FB:FBgn0002948

(Giunta et al., 2002)

involved_in gamma-tubulin complex localization

inferred from direct assay

(Romé and Ohkura, 2018)

involved_in male meiotic nuclear division

inferred from mutant phenotype

(assigned by UniProt )

(Giunta et al., 2002)

involved_in meiotic spindle midzone assembly

inferred from direct assay

(Jang et al., 2005)

involved_in meiotic spindle organization

inferred from mutant phenotype

(Giunta et al., 2002)

inferred from genetic interaction with FLYBASE:ncd; FB:FBgn0002924

(Giunta et al., 2002)

involved_in metaphase/anaphase transition of mitotic cell cycle

inferred from mutant phenotype

(Cesario et al., 2006)

involved_in microtubule nucleation

inferred from direct assay

(Romé and Ohkura, 2018)

involved_in mitotic cell cycle

inferred from mutant phenotype

(Giunta et al., 2002)

involved_in mitotic spindle organization

inferred from mutant phenotype

(Cesario et al., 2006)

involved_in spindle assembly involved in female meiosis

inferred from genetic interaction with FLYBASE:wac; FB:FBgn0035120

(Romé and Ohkura, 2018)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in microtubule-based movement

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (8 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

located_in meiotic spindle midzone

inferred from direct assay

(Romé and Ohkura, 2018)

part_of microtubule associated complex

inferred from direct assay

(assigned by UniProt )

(Giunta et al., 2002)

colocalizes_with spindle microtubule

inferred from direct assay

(Jang et al., 2007, Cesario et al., 2006)

located_in spindle midzone

inferred from direct assay

(Cesario et al., 2006, Jang et al., 2005)

located_in spindle pole

inferred from mutant phenotype

(assigned by UniProt )

(Giunta et al., 2002)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

part_of kinesin complex

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in microtubule

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN001189478

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

KINESINS

Protein Family (UniProt)

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. (Q9V877)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

KINESINS -: Kinesin superfamily proteins (KIFs) are microtubule motor proteins which use the hydrolysis of ATP to drive directional movement along microtubules. KIFs possess a well-conserved 360 residue globular head domain which binds and hydrolyses ATP and interacts with microtubules. Many KIFs homodimerize via coiled-coil interactions in the stalk region. KIFs bind cargo through their variable tail regions and are involved in transporting organelles, protein complexes, mRNAs and the movement of spindles and chromosomes during cell division. (Adapted from FBrf0219884).

Protein Function (UniProtKB)

Required during female meiosis for bipolar spindle formation in the absence of the centrosomes and chromosome homolog segregation. Also has roles in male meiosis and mitotic divisions of the early embryo.

(UniProt, Q9V877)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

sub: subito (T. Schupbach)

Maternal-effect lethal or female sterile; homozygous females lay eggs which show no visible signs of development when observed under transmitted light in a stereo microscope. These eggs are defective in fertilization or very early embryonic development.

Summary (Interactive Fly)

kinesin-6 homolog - bundles antiparallel microtubules - required for cytokinesis in mitosis and spindle organization and chromosome segregation in female meiosis

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\sub for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V877)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

sub-RA

FBtr0086881

NM_137416

2460

628

sub-RB

FBtr0340277

NM_001299619

2123

628

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

sub-PA

FBpp0086041

71.4

628

5.12

FBpp0309241

71.4

628

5.12

Q9V877

NP_001286548

AHN56344

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

628 aa isoforms: sub-PA, sub-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\sub using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

sub_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:

low

high

Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.

Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:

low

high

as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in meiotic spindle midzone

inferred from direct assay

(Romé and Ohkura, 2018)

part_of microtubule associated complex

inferred from direct assay

(assigned by UniProt )

(Giunta et al., 2002)

colocalizes_with spindle microtubule

inferred from direct assay

(Jang et al., 2007, Cesario et al., 2006)

located_in spindle midzone

inferred from direct assay

(Cesario et al., 2006, Jang et al., 2005)

located_in spindle pole

inferred from mutant phenotype

(assigned by UniProt )

(Giunta et al., 2002)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\sub in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\sub

View exonic expression by tissue for Dmel\sub

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44870/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003545

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003545

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003545

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12298

Flygut - An atlas of the Drosophila adult midgut

FBgn0003545

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 15 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 28 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of sub

Transgenic constructs containing regulatory region of sub

Aberrations (Deficiencies and Duplications) ( 9 )

Inferred from experimentation ( 9 )

Gene disrupted in

Df(2R)BSC347

(Kahsai et al., 2016, Christensen and Cook, 2007.10.29)

Df(2R)BSC348

(Christensen and Cook, 2007.10.29)

Df(2R)Exel7150

(Ryder, 2004.4.26)

Df(2R)PC4

(Giunta et al., 2002, Doane, 1996, Tearle, 1996.5.14, Moore et al., 1994, Schupbach and Wieschaus, 1991, Schupbach and Wieschaus, 1989)

Df(2R)Pcl^XM82

(Giunta et al., 2002, Tearle, 1996.5.14)

Gene not disrupted in

Df(2R)PC29

(Giunta et al., 2002)

Df(2R)Pcl7B

(Giunta et al., 2002, Tearle, 1996.5.14)

Df(2R)Pcl11B

(Giunta et al., 2002, Tearle, 1996.5.14)

Df(2R)RM2-1

(Giunta et al., 2002)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | maternal effect | recessive

lethal | recessive

sub^Dub

lethal - all die before end of pupal stage | recessive | heat sensitive

sub^Dub

lethal - all die during embryonic stage | non-rescuable maternal effect | recessive

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

sub^GD7559

viable, with Scer\GAL4^Mef2.PR

sub^GD7559

viable, with Scer\GAL4^pnr-MD237

sub^GD7559

viable, with Scer\GAL4^tin.CΔ4

sub^GD7559

Sterility

Allele

female semi-fertile (with sub¹⁷⁹⁴)

sub^Dub

female semi-fertile (with sub^Dub)

sub¹⁷⁹⁴

female semi-fertile | recessive

female sterile

sub^Dub

female sterile, with Scer\GAL4^VP16.nos.UTR

sub^{ΔNT.UASp.EGFP}

female sterile, with Scer\GAL4^VP16.nos.UTR, sub¹⁷⁹⁴/sub¹

sub^CT.UASp.EGFP

female sterile (with Df(2R)BSC347)

sub¹

female sterile (with sub¹)

sub¹³¹

female sterile (with sub¹), with Scer\GAL4^VP16.nos.UTR, sub^CT.UASp.EGFP

sub¹⁷⁹⁴

female sterile (with sub²²)

sub^Dub

female sterile (with sub⁷²)

sub^Dub

female sterile (with sub¹⁰⁴)

sub^Dub

female sterile (with sub¹³¹)

female sterile (with sub¹⁵⁸)

sub^Dub

female sterile (with sub²⁰²)

sub^Dub

female sterile (with sub²¹⁸)

sub^Dub

female sterile (with sub¹⁷⁹⁴), with Scer\GAL4^VP16.nos.UTR, sub^CT.UASp.EGFP

sub¹

female sterile (with sub^Dub)

female sterile | recessive

male sterile

sub^Dub

Other Phenotypes

Allele

abnormal locomotor behavior | adult stage

sub^Dub

abnormal meiotic cell cycle

sub^Dub

abnormal meiotic cell cycle (with Df(2R)Pcl^XM82)

sub¹⁷⁹⁴

abnormal meiotic cell cycle (with sub¹)

sub¹⁷⁹⁴

abnormal meiotic cell cycle (with sub¹⁷⁹⁴)

abnormal meiotic cell cycle (with sub^Dub)

sub¹⁷⁹⁴

abnormal meiotic cell cycle | dominant | heat sensitive

sub^Dub

abnormal meiotic cell cycle | oogenesis | oogenesis (with sub¹)

sub¹³¹

abnormal meiotic cell cycle | oogenesis | oogenesis (with sub¹³¹)

sub¹

abnormal meiotic cell cycle | recessive

abnormal mitotic cell cycle

sub¹³¹

abnormal mitotic cell cycle | larval stage (with sub¹)

sub¹³¹

abnormal mitotic cell cycle | larval stage (with sub¹³¹)

sub¹

abnormal mitotic cell cycle | maternal effect | recessive

melanotic mass phenotype | pupal stage

sub^Dub

short lived

sub^Dub

some die during embryonic stage | recessive

some die during pupal stage | recessive | heat sensitive

sub^Dub

visible, with Scer\GAL4^pnr-MD237

sub^GD7559

visible (with sub²²)

sub^Dub

visible (with sub⁷²)

sub^Dub

visible (with sub¹⁰⁴)

sub^Dub

visible (with sub¹³¹)

sub^Dub

visible (with sub¹⁵⁸)

sub^Dub

visible (with sub²⁰²)

sub^Dub

visible (with sub²¹⁸)

sub^Dub

visible (with sub^Dub)

visible | adult stage

sub^Dub

Phenotype manifest in

Allele

abdominal tergite 1 | heat sensitive

sub^Dub

abdominal tergite 2 | heat sensitive

sub^Dub

abdominal tergite 3 | heat sensitive

sub^Dub

abdominal tergite 4 | heat sensitive

sub^Dub

abdominal tergite 5 | heat sensitive

sub^Dub

abdominal tergite 6 | heat sensitive

sub^Dub

abdominal tergite 7 | heat sensitive

sub^Dub

brain | larval stage

sub¹³¹

brain | larval stage (with sub¹)

sub¹³¹

brain | larval stage (with sub¹³¹)

sub¹

chaeta | increased number, with Scer\GAL4^pnr-MD237

sub^GD7559

chromosome, centromeric region (with Df(2R)PC4)

sub¹⁷⁹⁴

chromosome | oogenesis | oogenesis, with Scer\GAL4^{VP16.mat.αTub67C}

sub^GL00583

eye (with sub²²)

sub^Dub

eye (with sub⁷²)

sub^Dub

eye (with sub¹⁰⁴)

sub^Dub

eye (with sub¹³¹)

sub^Dub

eye (with sub¹⁵⁸)

sub^Dub

eye (with sub²⁰²)

sub^Dub

eye (with sub²¹⁸)

sub^Dub

eye (with sub^Dub)

eye | heat sensitive

sub^Dub

imaginal disc | larval stage

sub^Dub

karyosome

sub¹⁷⁹⁴

karyosome, with Scer\GAL4^VP16.nos.UTR

sub^{ΔNT.UASp.EGFP}

karyosome (with Df(2R)Pcl^XM82)

sub¹⁷⁹⁴

karyosome (with sub¹⁷⁹⁴)

sub^Dub

karyosome (with sub^Dub)

sub¹⁷⁹⁴

macrochaeta | heat sensitive

sub^Dub

meiotic spindle | oogenesis, with Scer\GAL4^{VP16.mat.αTub67C}

sub^GL00583

meiotic spindle | oogenesis (with sub²)

sub¹³¹

meiotic spindle | oogenesis (with sub¹³¹)

sub²

oocyte, with Scer\GAL4^VP16.nos.UTR

sub^{ΔNT.UASp.EGFP}

oocyte (with sub¹)

sub¹³¹

oocyte (with sub²)

sub¹³¹

oocyte (with sub¹³¹)

oocyte | oogenesis stage S14, with Scer\GAL4^VP16.nos.UTR

sub^{ΔNT.UASp.EGFP}

spindle

spindle, with Scer\GAL4^{VP16.mat.αTub67C}

sub^GL00583

spindle (with Df(2R)Pcl^XM82)

sub¹⁷⁹⁴

spindle (with sub¹⁷⁹⁴)

sub^Dub

spindle (with sub^Dub)

sub¹⁷⁹⁴

spindle & oocyte

sub¹³¹

spindle & oocyte (with sub¹)

sub¹³¹

spindle & oocyte (with sub¹³¹)

sub¹

spindle | ectopic, with Scer\GAL4^VP16.nos.UTR

sub^{ΔNT.UASp.EGFP}

spindle microtubule & oocyte, with Scer\GAL4^VP16.nos.UTR

spindle microtubule | larval stage

sub¹³¹

spindle microtubule | larval stage (with sub¹)

sub¹³¹

spindle microtubule | larval stage (with sub¹³¹)

sub¹

spindle microtubule | oogenesis stage S14, with Scer\GAL4^VP16.nos.UTR

sub^{ΔNT.UASp.EGFP}

spindle midzone & oocyte

sub¹³¹

spindle pole & oocyte

sub¹

trichogen cell, with Scer\GAL4^pnr-MD237

sub^GD7559

wing (with sub²²)

sub^Dub

wing (with sub⁷²)

sub^Dub

wing (with sub¹⁰⁴)

sub^Dub

wing (with sub¹³¹)

sub^Dub

wing (with sub¹⁵⁸)

sub^Dub

wing (with sub²⁰²)

sub^Dub

wing (with sub²¹⁸)

sub^Dub

wing (with sub^Dub)

wing | heat sensitive

sub^Dub

wing margin (with sub²²)

sub^Dub

wing margin (with sub⁷²)

sub^Dub

wing margin (with sub¹⁰⁴)

sub^Dub

wing margin (with sub¹³¹)

sub^Dub

wing margin (with sub¹⁵⁸)

sub^Dub

wing margin (with sub²⁰²)

sub^Dub

wing margin (with sub²¹⁸)

sub^Dub

wing margin (with sub^Dub)

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\KIF20A

8 of 15

Yes

Hsap\KIF20B

4 of 15

Yes

Hsap\KIF23

3 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Kif20a

8 of 15

Yes

Mmus\Kif20b

4 of 15

Yes

Mmus\Kif23

2 of 15

Rattus norvegicus (Norway rat) (3)

Rnor\Kif20a

6 of 13

Yes

Rnor\Kif20b

3 of 13

Yes

Rnor\Kif23

2 of 13

Xenopus tropicalis (Western clawed frog) (4)

Xtro\kif20a

5 of 12

Yes

Xtro\kif23

2 of 12

Xtro\LOC100489788

2 of 12

Yes

Xtro\kif20b

1 of 12

Yes

Danio rerio (Zebrafish) (6)

Drer\kif20a

8 of 15

Yes

Drer\kif20ba

6 of 15

Yes

Drer\zgc:56231

5 of 15

Yes

Drer\kif20bb

4 of 15

Yes

Drer\kif23

3 of 15

Drer\si:dkey-96l17.6

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\zen-4

3 of 15

Yes

Cele\klp-7

1 of 15

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\SMY1

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\klp9

7 of 12

Yes

Spom\klp8

1 of 12

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (26)

Gene Symbol

Score

Source

Alignment

pav

5 of 10

CG32318

3 of 10

cana

2 of 10

cmet

2 of 10

cos

2 of 10

Khc-73

2 of 10

Khc

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

ncd

2 of 10

neb

2 of 10

nod

2 of 10

unc-104

2 of 10

CG5004

1 of 10

CG14535

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

KIF20A; kinesin family member 20A

8 of 15

605664

CARDIOMYOPATHY, FAMILIAL RESTRICTIVE, 6; RCM6

KIF20B; kinesin family member 20B

4 of 15

605498

KIF23; kinesin family member 23

3 of 15

605064

ANEMIA, CONGENITAL DYSERYTHROPOIETIC, TYPE IIIa; CDAN3A

congenital dyserythropoietic anemia type III

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

sub - Grip71

anti tag coimmunoprecipitation, western blot

(Romé and Ohkura, 2018)

sub - Trm1

experimental knowledge based

(Guruharsha et al., 2011)

sub - polo

anti tag coimmunoprecipitation, western blot, anti tag western blot

(Cesario et al., 2006)

sub - γTub23C

anti tag coimmunoprecipitation, western blot

(Romé and Ohkura, 2018)

sub - γTub37C

anti tag coimmunoprecipitation, western blot

(Romé and Ohkura, 2018)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

sub

suppressible

BubR1

(Cesario et al., 2006)

sub

enhanceable

Incenp

(Cesario et al., 2006)

sub

suppressible

ncd

(Cesario et al., 2006)

sub

enhanceable

ncd

(Giunta et al., 2002)

sub

enhanceable

nod

(Giunta et al., 2002)

sub

enhanceable

polo

(Cesario et al., 2006)

sub

suppressible

Ran

(Cesario and McKim, 2011)

sub

enhanceable

tacc

(Jang et al., 2005)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Klp61F

enhanceable

sub

(Radford et al., 2017)

ncd

enhanceable

sub

(Giunta et al., 2002)

nod

enhanceable

sub

(Giunta et al., 2002)

Ran

suppressible

sub

(Cesario and McKim, 2011)

Spc105R

enhanceable

sub

(Radford et al., 2015)

tacc

enhanceable

sub

(Jang et al., 2005)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

69344

DroID - A comprehensive database of gene and protein interactions.

FBgn0003545

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0003545

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-84

Cytogenetic map

54E7-54E7

Sequence location

2R:17,745,500..17,748,078 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

54E7-54E7

Limits computationally determined from genome sequence between P{lacW}l(2)k07509^k07509&P{EP}CG10934^EP538 and P{EP}sub^EP616

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-84

(FlyBase, 2016)

2-86.8

(Comeron et al., 2012)

2-82.6

(Moore et al., 1994)

2-86

(Schupbach and Wieschaus, 1989)

Left of (cM)

(Giunta et al., 2002)

(Moore et al., 1994)

Right of (cM)

(Giunta et al., 2002)

(Moore et al., 1994)

Notes

Mapping based on 33 recombinants.

(Moore et al., 1994)

Stocks and Reagents

Stocks (8)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

17186

w¹¹¹⁸; P{EP}sub^EP616

28570

y¹ v¹; P{TRiP.HM05056}attP2

81796

y¹ v¹; P{TKO.GS04842}attP40

5117

cn¹ sub¹ bw¹/CyO, l(2)DTS513¹

36623

y¹ sc^* v¹ sev²¹; P{TRiP.GL00583}attP2

Kyoto

108050

cn¹ sub¹ bw¹/CyO, l(2)DTS513¹

VDRC

v18754

w¹¹¹⁸ P{GD7559}v18754

v45402

w¹¹¹⁸; P{GD7559}v45402/TM3

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (97)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD35138

Other clones

DPiM_LD35138

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003545

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003545

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

44870

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: mei-1794 CG12298

(McKim, 2001.3.5)

Source for database merge of

Source for merge of: sub Dub mei-1794

(Giunta et al., 2002)

Additional comments

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R⁺ cells: cell morphology is aberrant and there is an increased frequency of microtubule-based mitotic spindles, indicative of a failure in mitosis. This phenotype is not seen in Kc167 cells.

(Kiger et al., 2003)

sub is required for bipolar spindle formation. sub is dispensable for most mitotic divisions, though not the early embryonic divisions.

(Giunta et al., 2002)

In sub mutants spindles can be unipolar, multipolar or frayed with no defined poles. sub mutants do not interact with alleles of γTub37C, αTub67C, msps, tacc or wisp.

(Giunta et al., 2002)

Identification: genetic screen for meiotic mutants.

(Manheim et al., 2001)

Required for spindle assembly during meiosis, and specifically, the formation of the two spindle poles.

(Manheim et al., 2001)

Mutants show defective meiosis.

(Manheim et al., 2001)

Mutations of sub primarily affect the homolog segregation during the first meiotic division of both males and females (FBrf0073960).

(Moore and Orr-Weaver, 1995)

sub is unusual in that mutations cause aberrant chromosome segregation almost exclusively in meiosis I in both sexes.

(Moore et al., 1994)

Mutations in sub show a strict maternal effect phenotype with defects in fertilisation or early embryonic development.

(Schupbach and Wieschaus, 1989)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 42 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44870

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V877

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V877

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4LGW1

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V877

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003545

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44870/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003545

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

sub_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003545

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003545

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12298

Flygut - An atlas of the Drosophila adult midgut

FBgn0003545

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0003545

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

44870

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013546

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG12298

MARRVEL_MODEL - MARRVEL (model organism gene)

44870

modMine - A data warehouse for the modENCODE project

FBgn0003545

Linkouts

BioGRID - A database of protein and genetic interactions.

69344

DroID - A comprehensive database of gene and protein interactions.

FBgn0003545

DRSC - Results frm RNAi screens

FBgn0003545

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003545

FlyMine - An integrated database for Drosophila genomics

FBgn0003545

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

CG12298

(Çiçek et al., 2016, Sing et al., 2014, Ni et al., 2011.7.19, Perkins et al., 2009, Hughes et al., 2008, Balasubramanian et al., 2004, Mohr and Gelbart, 2002, Miki et al., 2001)

DmKlp54E

(Dagenbach and Endow, 2004)

DmSub

(Hirokawa, 2012)

Dub

(Tomkiel, 2000, Williams et al., 1996, Moore and Orr-Weaver, 1995, Orr-Weaver, 1995, Carpenter, 1994, Moore et al., 1994)

KIF 20A

(Miki et al., 2001)

KIF20A/B

(Hirokawa, 2012.11.20)

MKLP2

(Verma and Maresca, 2019)

MKlp2

(Costa and Ohkura, 2019)

Mklp2

(Maiato et al., 2015, Afonso et al., 2014)

SUB

(Wu et al., 2008)

Sub

(Hirokawa, 2012.11.20, Miki et al., 2005)

Subito

(Romé and Ohkura, 2018)

mei-1794

(Li and White, 2003, McKim, 2001.3.5, Goldstein and Gunawardena, 2000)

sub

(Wang et al., 2021, Kochanova et al., 2020, Witte et al., 2020, Hughes et al., 2018, Radford et al., 2017, Transgenic RNAi Project members, 2017-, Das et al., 2016, Radford et al., 2015, Puerma et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Radford et al., 2012, Radford et al., 2012, Cesario and McKim, 2011, Wu et al., 2008, Christensen and Cook, 2007.10.29, Christensen and Cook, 2007.10.29, Jang et al., 2007, Swan and Schupbach, 2007, Jang et al., 2005)

subito

(Colombié et al., 2008)

Name Synonyms

Double or nothing

(Moore and Orr-Weaver, 1995, Moore et al., 1994)

Kinesin_A

(Kollmar, 2015.9.15)

Subito

(Costa and Ohkura, 2019, Radford et al., 2017, Maiato et al., 2015, Afonso et al., 2014, Hirokawa, 2012.11.20, Radford et al., 2012, Cesario and McKim, 2011, Cesario and McKim, 2008, Wu et al., 2008, Swan and Schupbach, 2007, Jang et al., 2006)

double-or-nothing

(Philp, 1998)

subito

(Das et al., 2016, Puerma et al., 2014, Reis et al., 2011, Anderson et al., 2009, Fisher et al., 2008, Jang et al., 2007, Cesario et al., 2006, Jang et al., 2005)

Secondary FlyBase IDs

FBgn0013911
FBgn0034273

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

References (99)

The following external sites may use different assemblies or annotations than FlyBase.

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