• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Tbp

General Information
Symbol	Dmel\Tbp	Species	D. melanogaster
Name	TATA binding protein	Annotation symbol	CG9874
Feature type	protein_coding_gene	FlyBase ID	FBgn0003687
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	TFIID, dTBP, TFIIDτ, dTFIID
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	57F8-57F8	Sequence location	2R:17,573,705..17,575,145 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene TATA binding protein is referred to in FlyBase by the symbol Dmel\Tbp (CG9874, FBgn0003687). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly; transcription factor binding; TFIIA-class transcription factor binding; sequence-specific core promoter binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly. There is experimental evidence that it is involved in the biological process: transcription initiation from RNA polymerase II promoter; snRNA transcription from RNA polymerase III promoter; regulation of transcription from RNA polymerase III promoter; tRNA transcription from RNA polymerase III promoter. 30 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Beta2-adaptin/TATA-box binding, C-terminal; TATA-box binding protein; Transcription factor TFIID, C-terminal/DNA glycosylase, N-terminal. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1637663_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Tbp. Gene sequence location is 2R:17573705..17575145.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10	Transgenic Constructs P{UAS-Tbp.R}
FB2012_01	References Theisen et al., 2010, Mol. Cell. Biol. 30(14): 3471--3479 Vorobyeva et al., 2009, Cell Cycle 8(14): 2152--2156 Yokomori et al., 1993, Genes Dev. 7(12B): 2587--2597 Controlled Vocabulary Terms TFIIA-class transcription factor binding TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly sequence-specific core promoter binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly Sequence features HFA04662 BKN20836
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 57F8-57F8   Limits computationally determined from genome sequence between P{EP}CG10082EP436&P{EP}EP712EP712 and P{EP}Tim10EP541&P{lacW}l(2)k09617k09617
Experimentally Determined Cytological Location
Cytogenetic map Notes References 57F8-57F10   (determined by in situ hybridisation)   (Hoey et al., 1990)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Tbp for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Tbp-RA FBtr0071679  NM_079081   1377   353
Additional Transcript Data & Comments
Reported size (kB)	1.6 (northern blot) (Hoey et al., 1990)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Tbp-PA   FBpp0071596   38.5   353   9.79     NP_523805   AAF46754
Additional Polypeptide Data & Comments
Reported size (kDa)	353 (aa); 38 (kD predicted) (Muhich et al., 1990, Hoey et al., 1990)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • TATA-box binding protein (IPR000814) Transcription factor TFIID, C-terminal/DNA glycosylase, N-terminal (IPR012294) Beta2-adaptin/TATA-box binding, C-terminal (IPR012295)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA802793   AA952022   AE013599 AAF46754   AI512297   AW943695   AY069663 AAL39808   BG635541   BI165814   BI358167   BI638511   BI641849   BQ102720   CK604483   CO176801   CO185992   CO271538   CO313366   CO332330   CO335305   EC070153   EC078550   EC080163   EC088083   EC090970   EC197615   EC205450   EC217583   EC251113   EL886533   EV582084   M38082 AAA28931   M38388 AAA28926   U11718 AAA68629   U35147 AAA79092
UniProtKB/Swiss-Prot	P20227
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Hoey et al., 1990)
Additional Descriptive Data
	Tbp transcripts are detected at a constant level throughout embryogenesis. (Hoey et al., 1990)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0003687    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0003687 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0003687 expression level Developmental Stage   Expression Level embryo 00-02hr    4981 embryo 02-04hr    3078 embryo 04-06hr    3099 embryo 06-08hr    2589 embryo 08-10hr    1908 embryo 10-12hr    1262 embryo 12-14hr    1136 embryo 14-16hr    708 embryo 16-18hr    514 embryo 18-20hr    431 embryo 20-22hr    433 embryo 22-24hr    410 larva L1    785 larva L2    627 larva L3 12hr old    491 larva L3 puffstage 1-2    548 larva L3 puffstage 3-6    743 larva L3 puffstage 7-9    890 white prepupae new    921 white prepupae 12hr    929 white prepupae 24hr    1009 pupae 2d postWPP    623 pupae 3d postWPP    350 pupae 4d postWPP    264 adult male 01day    368 adult male 05day    423 adult male 30day    476 adult female 01day    1192 adult female 05day    2391 adult female 30day    2434 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4981) embryo 02-04hr  (3078) embryo 04-06hr  (3099) embryo 06-08hr  (2589) embryo 08-10hr  (1908) embryo 10-12hr  (1262) embryo 12-14hr  (1136) embryo 14-16hr  (708) embryo 16-18hr  (514) embryo 18-20hr  (431) embryo 20-22hr  (433) embryo 22-24hr  (410) larva L1  (785) larva L2  (627) larva L3 12hr old  (491) larva L3 puffstage 1-2  (548) larva L3 puffstage 3-6  (743) larva L3 puffstage 7-9  (890) white prepupae new  (921) white prepupae 12hr  (929) white prepupae 24hr  (1009) pupae 2d postWPP  (623) pupae 3d postWPP  (350) pupae 4d postWPP  (264) adult male 01day  (368) adult male 05day  (423) adult male 30day  (476) adult female 01day  (1192) adult female 05day  (2391) adult female 30day  (2434) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4981) embryo 02-04hr  (3078) embryo 04-06hr  (3099) embryo 06-08hr  (2589) embryo 08-10hr  (1908) embryo 10-12hr    1262 embryo 12-14hr    1136 embryo 14-16hr    708 embryo 16-18hr    514 embryo 18-20hr    431 embryo 20-22hr    433 embryo 22-24hr    410 larva L1    785 larva L2    627 larva L3 12hr old    491 larva L3 puffstage 1-2    548 larva L3 puffstage 3-6    743 larva L3 puffstage 7-9    890 white prepupae new    921 white prepupae 12hr    929 white prepupae 24hr    1009 pupae 2d postWPP    623 pupae 3d postWPP    350 pupae 4d postWPP    264 adult male 01day    368 adult male 05day    423 adult male 30day    476 adult female 01day    1192 adult female 05day  (2391) adult female 30day  (2434) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4981 embryo 02-04hr    3078 embryo 04-06hr    3099 embryo 06-08hr    2589 embryo 08-10hr    1908 embryo 10-12hr    1262 embryo 12-14hr    1136 embryo 14-16hr    708 embryo 16-18hr    514 embryo 18-20hr    431 embryo 20-22hr    433 embryo 22-24hr    410 larva L1    785 larva L2    627 larva L3 12hr old    491 larva L3 puffstage 1-2    548 larva L3 puffstage 3-6    743 larva L3 puffstage 7-9    890 white prepupae new    921 white prepupae 12hr    929 white prepupae 24hr    1009 pupae 2d postWPP    623 pupae 3d postWPP    350 pupae 4d postWPP    264 adult male 01day    368 adult male 05day    423 adult male 30day    476 adult female 01day    1192 adult female 05day    2391 adult female 30day    2434 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4981 embryo 02-04hr    3078 embryo 04-06hr    3099 embryo 06-08hr    2589 embryo 08-10hr    1908 embryo 10-12hr    1262 embryo 12-14hr    1136 embryo 14-16hr    708 embryo 16-18hr    514 embryo 18-20hr    431 embryo 20-22hr    433 embryo 22-24hr    410 larva L1    785 larva L2    627 larva L3 12hr old    491 larva L3 puffstage 1-2    548 larva L3 puffstage 3-6    743 larva L3 puffstage 7-9    890 white prepupae new    921 white prepupae 12hr    929 white prepupae 24hr    1009 pupae 2d postWPP    623 pupae 3d postWPP    350 pupae 4d postWPP    264 adult male 01day    368 adult male 05day    423 adult male 30day    476 adult female 01day    1192 adult female 05day    2391 adult female 30day    2434 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0003687 expression level Developmental Stage   Expression Level embryo 00-02hr    4981 embryo 02-04hr    3078 embryo 04-06hr    3099 embryo 06-08hr    2589 embryo 08-10hr    1908 embryo 10-12hr    1262 embryo 12-14hr    1136 embryo 14-16hr    708 embryo 16-18hr    514 embryo 18-20hr    431 embryo 20-22hr    433 embryo 22-24hr    410 larva L1    785 larva L2    627 larva L3 12hr old    491 larva L3 puffstage 1-2    548 larva L3 puffstage 3-6    743 larva L3 puffstage 7-9    890 white prepupae new    921 white prepupae 12hr    929 white prepupae 24hr    1009 pupae 2d postWPP    623 pupae 3d postWPP    350 pupae 4d postWPP    264 adult male 01day    368 adult male 05day    423 adult male 30day    476 adult female 01day    1192 adult female 05day    2391 adult female 30day    2434 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4981) embryo 02-04hr  (3078) embryo 04-06hr  (3099) embryo 06-08hr  (2589) embryo 08-10hr  (1908) embryo 10-12hr  (1262) embryo 12-14hr  (1136) embryo 14-16hr  (708) embryo 16-18hr  (514) embryo 18-20hr  (431) embryo 20-22hr  (433) embryo 22-24hr  (410) larva L1  (785) larva L2  (627) larva L3 12hr old  (491) larva L3 puffstage 1-2  (548) larva L3 puffstage 3-6  (743) larva L3 puffstage 7-9  (890) white prepupae new  (921) white prepupae 12hr  (929) white prepupae 24hr  (1009) pupae 2d postWPP  (623) pupae 3d postWPP  (350) pupae 4d postWPP  (264) adult male 01day  (368) adult male 05day  (423) adult male 30day  (476) adult female 01day  (1192) adult female 05day  (2391) adult female 30day  (2434) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4981) embryo 02-04hr  (3078) embryo 04-06hr  (3099) embryo 06-08hr  (2589) embryo 08-10hr  1908 embryo 10-12hr    1262 embryo 12-14hr    1136 embryo 14-16hr    708 embryo 16-18hr    514 embryo 18-20hr    431 embryo 20-22hr    433 embryo 22-24hr    410 larva L1    785 larva L2    627 larva L3 12hr old    491 larva L3 puffstage 1-2    548 larva L3 puffstage 3-6    743 larva L3 puffstage 7-9    890 white prepupae new    921 white prepupae 12hr    929 white prepupae 24hr    1009 pupae 2d postWPP    623 pupae 3d postWPP    350 pupae 4d postWPP    264 adult male 01day    368 adult male 05day    423 adult male 30day    476 adult female 01day    1192 adult female 05day  2391 adult female 30day  2434 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4981 embryo 02-04hr    3078 embryo 04-06hr    3099 embryo 06-08hr    2589 embryo 08-10hr    1908 embryo 10-12hr    1262 embryo 12-14hr    1136 embryo 14-16hr    708 embryo 16-18hr    514 embryo 18-20hr    431 embryo 20-22hr    433 embryo 22-24hr    410 larva L1    785 larva L2    627 larva L3 12hr old    491 larva L3 puffstage 1-2    548 larva L3 puffstage 3-6    743 larva L3 puffstage 7-9    890 white prepupae new    921 white prepupae 12hr    929 white prepupae 24hr    1009 pupae 2d postWPP    623 pupae 3d postWPP    350 pupae 4d postWPP    264 adult male 01day    368 adult male 05day    423 adult male 30day    476 adult female 01day    1192 adult female 05day    2391 adult female 30day    2434 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4981 embryo 02-04hr    3078 embryo 04-06hr    3099 embryo 06-08hr    2589 embryo 08-10hr    1908 embryo 10-12hr    1262 embryo 12-14hr    1136 embryo 14-16hr    708 embryo 16-18hr    514 embryo 18-20hr    431 embryo 20-22hr    433 embryo 22-24hr    410 larva L1    785 larva L2    627 larva L3 12hr old    491 larva L3 puffstage 1-2    548 larva L3 puffstage 3-6    743 larva L3 puffstage 7-9    890 white prepupae new    921 white prepupae 12hr    929 white prepupae 24hr    1009 pupae 2d postWPP    623 pupae 3d postWPP    350 pupae 4d postWPP    264 adult male 01day    368 adult male 05day    423 adult male 30day    476 adult female 01day    1192 adult female 05day    2391 adult female 30day    2434 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0003687    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0003687 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0003687 expression level Tissue   Expression Level Larval Central Nervous System    108.175 Larval Midgut no informative data Larval Hindgut    42.1 Larval Malpighian Tubules    43.3 Larval Fat Body    34.6 Larval Salivary Gland    84.9 Larval Trachea    86.575 Larval Carcass    41.075 Adult Head    36.4 Adult Eye    47.55 Adult Brain    70.7 Adult Thoracic-Abdominal Ganglion    59.7 Adult Crop    57.3 Adult Midgut    38.7 Adult Hindgut    44.9 Adult Malpighian Tubules    45.4 Adult Fat Body no informative data Adult Salivary Gland    78.2 Adult Heart    36.075 Adult VirginFemale Spermatheca    25.8 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary    240.9 Adult Testis    16.5 Adult Male Accessory Gland    61.5 Adult Carcass    30.1 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    108.175 Larval Midgut no informative data Larval Hindgut    42.1 Larval Malpighian Tubules    43.3 Larval Fat Body    34.6 Larval Salivary Gland    84.9 Larval Trachea    86.575 Larval Carcass    41.075 Adult Head    36.4 Adult Eye    47.55 Adult Brain    70.7 Adult Thoracic-Abdominal Ganglion    59.7 Adult Crop    57.3 Adult Midgut    38.7 Adult Hindgut    44.9 Adult Malpighian Tubules    45.4 Adult Fat Body no informative data Adult Salivary Gland    78.2 Adult Heart    36.075 Adult VirginFemale Spermatheca    25.8 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary    240.9 Adult Testis    16.5 Adult Male Accessory Gland    61.5 Adult Carcass    30.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    108.175 Larval Midgut no informative data Larval Hindgut    42.1 Larval Malpighian Tubules    43.3 Larval Fat Body    34.6 Larval Salivary Gland    84.9 Larval Trachea    86.575 Larval Carcass    41.075 Adult Head    36.4 Adult Eye    47.55 Adult Brain    70.7 Adult Thoracic-Abdominal Ganglion    59.7 Adult Crop    57.3 Adult Midgut    38.7 Adult Hindgut    44.9 Adult Malpighian Tubules    45.4 Adult Fat Body no informative data Adult Salivary Gland    78.2 Adult Heart    36.075 Adult VirginFemale Spermatheca    25.8 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary    240.9 Adult Testis    16.5 Adult Male Accessory Gland    61.5 Adult Carcass    30.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    108.175 Larval Midgut no informative data Larval Hindgut    42.1 Larval Malpighian Tubules    43.3 Larval Fat Body    34.6 Larval Salivary Gland    84.9 Larval Trachea    86.575 Larval Carcass    41.075 Adult Head    36.4 Adult Eye    47.55 Adult Brain    70.7 Adult Thoracic-Abdominal Ganglion    59.7 Adult Crop    57.3 Adult Midgut    38.7 Adult Hindgut    44.9 Adult Malpighian Tubules    45.4 Adult Fat Body no informative data Adult Salivary Gland    78.2 Adult Heart    36.075 Adult VirginFemale Spermatheca    25.8 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary    240.9 Adult Testis    16.5 Adult Male Accessory Gland    61.5 Adult Carcass    30.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0003687 expression level Tissue   Expression Level Larval Central Nervous System    108.175 Larval Midgut no informative data Larval Hindgut    42.1 Larval Malpighian Tubules    43.3 Larval Fat Body    34.6 Larval Salivary Gland    84.9 Larval Trachea    86.575 Larval Carcass    41.075 Adult Head    36.4 Adult Eye    47.55 Adult Brain    70.7 Adult Thoracic-Abdominal Ganglion    59.7 Adult Crop    57.3 Adult Midgut    38.7 Adult Hindgut    44.9 Adult Malpighian Tubules    45.4 Adult Fat Body no informative data Adult Salivary Gland    78.2 Adult Heart    36.075 Adult VirginFemale Spermatheca    25.8 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary    240.9 Adult Testis    16.5 Adult Male Accessory Gland    61.5 Adult Carcass    30.1 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    108.175 Larval Midgut no informative data Larval Hindgut    42.1 Larval Malpighian Tubules    43.3 Larval Fat Body    34.6 Larval Salivary Gland    84.9 Larval Trachea    86.575 Larval Carcass    41.075 Adult Head    36.4 Adult Eye    47.55 Adult Brain    70.7 Adult Thoracic-Abdominal Ganglion    59.7 Adult Crop    57.3 Adult Midgut    38.7 Adult Hindgut    44.9 Adult Malpighian Tubules    45.4 Adult Fat Body no informative data Adult Salivary Gland    78.2 Adult Heart    36.075 Adult VirginFemale Spermatheca    25.8 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary    240.9 Adult Testis    16.5 Adult Male Accessory Gland    61.5 Adult Carcass    30.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    108.175 Larval Midgut no informative data Larval Hindgut    42.1 Larval Malpighian Tubules    43.3 Larval Fat Body    34.6 Larval Salivary Gland    84.9 Larval Trachea    86.575 Larval Carcass    41.075 Adult Head    36.4 Adult Eye    47.55 Adult Brain    70.7 Adult Thoracic-Abdominal Ganglion    59.7 Adult Crop    57.3 Adult Midgut    38.7 Adult Hindgut    44.9 Adult Malpighian Tubules    45.4 Adult Fat Body no informative data Adult Salivary Gland    78.2 Adult Heart    36.075 Adult VirginFemale Spermatheca    25.8 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary    240.9 Adult Testis    16.5 Adult Male Accessory Gland    61.5 Adult Carcass    30.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    108.175 Larval Midgut no informative data Larval Hindgut    42.1 Larval Malpighian Tubules    43.3 Larval Fat Body    34.6 Larval Salivary Gland    84.9 Larval Trachea    86.575 Larval Carcass    41.075 Adult Head    36.4 Adult Eye    47.55 Adult Brain    70.7 Adult Thoracic-Abdominal Ganglion    59.7 Adult Crop    57.3 Adult Midgut    38.7 Adult Hindgut    44.9 Adult Malpighian Tubules    45.4 Adult Fat Body no informative data Adult Salivary Gland    78.2 Adult Heart    36.075 Adult VirginFemale Spermatheca    25.8 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary    240.9 Adult Testis    16.5 Adult Male Accessory Gland    61.5 Adult Carcass    30.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    36.4   NA  Eye    47.55   NA  Brain    70.7   108.175  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    59.7   NA  Crop    57.3   no informative data  Midgut    38.7   42.1  Hindgut    44.9   43.3  Malpighian Tubules    45.4   34.6  Fat Body    no informative data   84.9  Salivary Gland    78.2   NA  Heart    36.075   86.575  Trachea    NA   NA  VirginFemale Spermatheca    25.8   NA  InseminatedFemale Spermatheca    22.9   NA  Ovary    240.9   NA  Testis    16.5   NA  Male Accessory Gland    61.5   41.075  Carcass    30.1 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_13
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003687 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9874-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | first instar larval stage | recessive Tbpf00190 lethal | second instar larval stage (with Tbpf00190), with Scer\GAL4hs.PB TbpScer\UAS.cRa lethal | second instar larval stage (with TbpScer\UAS.cRa), with Scer\GAL4hs.PB Tbpf00190 Phenotype manifest in Allele
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of Tbp Class Mutagen Stocks Known lesion Tbpf00190 piggyBac transposase 3 -- TbpE-3911 0 -- TbpsBT1 X ray 0 -- TbpsC4 ethyl methanesulfonate 0 -- TbpsEU4 X ray 0 -- TbpsI10 ethyl methanesulfonate 0 -- TbpsO12 ethyl methanesulfonate 0 -- TbpsP5a ethyl methanesulfonate 0 --
Alleles Carried on Transgenic Constructs ( 22 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Tbp Class Mutagen Stocks Known lesion Tbp1-157.T:Scer\GAL4 in vitro construct - coding region fusion 0 Yes Tbp1-282.T:Scer\GAL4 in vitro construct - coding region fusion 0 Yes Tbp163-353.T:Scer\GAL4 in vitro construct - coding region fusion 0 Yes Tbpa.cKa in vitro construct - RNAi 0 Yes TbpAct5C.cHa.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes TbpAct5C.PC in vitro construct - regulatory fusion 0 Yes TbpAct5C.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes TbpAct5C.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes TbpcKa in vitro construct - RNAi 0 Yes TbpdsRNA.cWa in vitro construct - RNAi 0 Yes Tbpfl.T:Scer\GAL4 in vitro construct - coding region fusion 0 Yes TbpGD4495 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Tbphs.T:Zzzz\FLAG in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes TbpKK100740 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes TbpMtnA.T:Zzzz\FLAG in vitro construct - coding region fusion 0 Yes TbpR332H.Act5C.T:Ivir\HA1 in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes TbpR332H.MtnA.T:Zzzz\FLAG in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes TbpScer\UAS.cRa in vitro construct - regulatory fusion 0 Yes TbpT:Ivir\HA1 in vitro construct - coding region fusion 0 Yes TbpΔ315-320.T:Scer\GAL4 in vitro construct - deletion 0 Yes TbpΔBgl.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes TbpΔNde.Act5C.T:Ivir\HA1 in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4495} NA P{KK100740} NA P{UAS-Tbp.R} NA heat-shock construct P{eTBP} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}Tbpf00190 NA
Gene Ontology: Function, Process & Cellular Component ( 15 unique terms )
Terms Based on Experimental Evidence ( 12 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with mbf1 (Liu et al., 2003) contributes_to sequence-specific core promoter binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly inferred from direct assay (Hoey et al., 1990) TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly inferred from physical interaction with Taf12 (Yokomori et al., 1993) TFIIA-class transcription factor binding inferred from physical interaction with TfIIA-L (Yokomori et al., 1993) transcription factor binding inferred from physical interaction with Taf1 (Kokubo et al., 1994) inferred from physical interaction with Taf105 (Vilalta et al., 1997)
Biological Process
CV term Evidence References
regulation of transcription from RNA polymerase III promoter inferred from mutant phenotype (Trivedi et al., 1996) snRNA transcription from RNA polymerase III promoter inferred from direct assay (Trivedi et al., 1996) transcription initiation from RNA polymerase II promoter inferred from direct assay (Hoey et al., 1990) tRNA transcription from RNA polymerase III promoter inferred from direct assay (Trivedi et al., 1996) inferred from direct assay AND inferred from mutant phenotype (Vilalta et al., 1997)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Hoey et al., 1990) transcription factor TFIID complex inferred from physical interaction with Taf4 (Wright et al., 2006) inferred from physical interaction with Taf6 (Wright et al., 2006) colocalizes_with transcription factor TFIIIB complex inferred from physical interaction with Taf105 (Vilalta et al., 1997)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from electronic annotation with InterPro:IPR000814, InterPro:IPR012294 (FlyBase Curators et al., 2004-) non-traceable author statement (Swiss-Prot Project Members, 1991.2.1) contributes_to sequence-specific core promoter binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly inferred from sequence or structural similarity with human TBP (Aoyagi and Wassarman, 2000) inferred from sequence or structural similarity with Saccharomyces SPT15 (Aoyagi and Wassarman, 2000)
Biological Process
CV term Evidence References
regulation of transcription from RNA polymerase II promoter non-traceable author statement (Swiss-Prot Project Members, 1991.2.1) traceable author statement (Davidson, 2003) transcription initiation from RNA polymerase II promoter inferred from sequence or structural similarity with human TBP AND inferred from sequence or structural similarity with human TBPL1 AND inferred from sequence or structural similarity with Saccharomyces SPT15 AND inferred from sequence or structural similarity with worm tbp-1 AND inferred from sequence or structural similarity with worm tlp-1 (Aoyagi and Wassarman, 2000)
Cellular Component
CV term Evidence References
DNA-directed RNA polymerase III complex inferred by curator from GO:0006359|GO:0042797|GO:0042796 (Trivedi et al., 1996) nucleus non-traceable author statement (Swiss-Prot Project Members, 1991.2.1) transcription factor TFIID complex inferred from sequence or structural similarity with human TBP AND inferred from sequence or structural similarity with human TBPL1 AND inferred from sequence or structural similarity with Saccharomyces SPT15 AND inferred from sequence or structural similarity with worm tbp-1 AND inferred from sequence or structural similarity with worm tlp-1 (Aoyagi and Wassarman, 2000) non-traceable author statement (Swiss-Prot Project Members, 1991.2.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Tbp allele Gene References TbpsBT1 N (Verheyen et al., 1996) TbpsC4 N (Verheyen et al., 1996) TbpsEU4 N (Verheyen et al., 1996) TbpsI10 N (Verheyen et al., 1996) unspecified N (Verheyen et al., 1996)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63101 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0003687 DroID - A comprehensive database of gene and protein interactions. • FBgn0003687 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37476
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11627 Dere\GG20753 Dgri\GH23121 Dmoj\GI18943 Dper\GL11271 Dpse\GA22088 Dsec\GM15696 Dsim\GD25175 Dvir\Tbp Dwil\GK10649 Dyak\GE13685
Curated drosophilid orthologs	Dpse\GA22088   Dvir\Tbp (Oh, 2000.1.3)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL012330-PA Aedes aegypti (mosquito) AGAP010958-PA Anopheles gambiae (mosquito) XP_623088.1 Apis mellifera (honey bee) XP_001949590.1 Acyrthosiphon pisum (pea aphid) At3g13445.1 Arabidopsis thaliana (wall cress) 90475 Batrachochytrium dendrobatidis (chytrid fungus) 121529 Branchiostoma floridae (lancelet) BGIBMGA010619-PA Bombyx mori (silk worm) ENSBTAP00000010109 Bos taurus (bovine) CAL0001658 Candida albicans (a form of yeast) CN12208 Caenorhabditis brenneri (small nematode) CBP02248 Caenorhabditis briggsae (nematode) CE01408 Caenorhabditis elegans (worm) ENSCAFP00000006103 Canis familiaris (dog) CAGL0I06688g Candida glabrata (haploid yeast) Chro.80238 Cryptosporidium hominis (obligate parasite) ENSCINP00000005464 Ciona intestinalis (sea squirt) JA18543 Caenorhabditis japonica (nematode) CMF095C Cyanidioschyzon merolae (red alga) cn12106 Cryptococcus neoformans (yeast-like fungus) cgd8_2030 Cryptosporidium parvum (protozoan parasite) CPIJ007600 Culex pipens (mosquito) CPIJ018368 Culex pipens (mosquito) ENSCPOP00000001072 Cavia porcellus (guinea pig) 100267 Chlamidomonas reinhardtii (green alga) RP20018 Caenorhabditis remanei (nematode) ENSCSAVP00000012157 Ciona savignyi (sedentary tunicate) 209597 Capitella spl (polychaete worm) DDB0185066 Dictyostelium discoideum (amoeba) DEHA2G11836g Debaryomyces Hansenii (yeast) 194512 Daphnia pulex (daphnia) ENSDARP00000010211 Danio rerio (zebra fish) ENSECAP00000006680 Equus caballus (horse) ENSGACP00000021304 Gymnopilus aculeatus (mushroom) ENSGALP00000036926 Gallus gallus (chicken) 86082 Hypsipyla robusta (cedar shoot borer) ENSP00000375942 Homo sapiens (human) ISCW019416-PA Ixodes scapularis (deer tick) KLLA0E10099g Kluyveromyces lactis (yeast) LmjF19.1390 Leishmania major (virus) ENSMODP00000006780 Monodelphis domestica (gray opossum) ENSMMUP00000020642 Macaca mulatta (rhesus monkey) ENSMUSP00000114007 Mus musculus (house mouse) 203375 Nematostella vectensis (starlet anemone) XP_001603648.1 Nasonia vitripennis (wasp) ENSOANP00000020337 Ornithorynchus anatinus (platypus) ENSORLP00000013489 Oryzias latipes (japanese killifish) LOC_Os10g29660.1 Oryza sativa (asian rice) 29198 Ostreococcus tauri (green alga) PHUM010718-PA Pediculus humanus (human body louse) 115252 Physcomitrella patens (moss) ENSPPYP00000019257 Pongo pygmaeus (orangutan) 84970 Phytophthora ramorum (sudden oak death pathogen) 129327 Phytophthora sojae (root rot pathogen) 730014 Populus trichocarpa (black cottonwood) ENSPTRP00000032146 Pan troglodytes (chimpanzee) ENSRNOP00000002038 Rattus norvegicus (norway rat) 4774638 Sorghum bicolor (sorghum) YER148W Saccharomyces cerevisiae (budding yeast) Smp_098950 Schistosoma mansoni (trematode parasite) SPAC29E6.08 Schizosaccharomyces pombe (fission yeast) NP_999786.1 Strongylocentrotus purpuratus (purple sea urchin) XP_795391.2 Strongylocentrotus purpuratus (purple sea urchin) 50358 Trichoplax adhaerens (metazoa) XP_969326.1 Tribolium castaneum (red flour beetle) Tc00.1047053503809.149 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000002633 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000017711 Takifugu rubripes (pufferfish Takifugu) 3812.m02620 Tetrahymena thermophila (ciliate protozoa) 89741.m00028 Trichomonas vaginalis (parasitic flagellated protozoan) 90019.m00264 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000018725 Xenopus tropicalis (frog) YALI0B23056g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • LH13241 ISCW019416 FBgn0231340 FBgn0212662 FBgn0202120 FBgn0041651 FBgn0196487 FBgn0170612 JGI_V11_194512 FBgn0082072 FBgn0148880 FBgn0141682 FBgn0130578 FBgn0112940 FBgn0088667 CPIJ007600 CPIJ018368 BGIBMGA010619 ACYPI005546 ACYPI001633 GB17946 AGAP010958 AAEL012330 PB20491 PHUM533080 GLEAN_08403
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	18301 w1118; PBac{WH}Tbpf00190/CyO
Harvard	- PBac{WH}Tbpf00190
Kyoto	114517 yd2 w1118 P{ey-FLP.N}2; P{neoFRT}42D PBac{WH}Tbpf00190 /CyO y+
Genomic Clones ( 3 )
	BACR13F14 BACR34C17 BACR46G10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 27 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD44083
Other clones	DPiM_LD44083
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM06325 LD29033 RE05773 RE44855 SD12983 SD20813 SD25492
Other clones	106733 176081 275289 283251 CK01.14C.H1 CK01.86D.A10 CK01.169B.F1 Dmel_dig_007665_0721_0837 EC090970 EK014922 EK051032 EK103821 EK232439 EK303154 EK316751 IP17711 UT01228 UUGC0104
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003687 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37476
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	DNA-protein interactions: genome-wide binding profile assayed for Tbp protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069). (Filion et al., 2010) dsRNA has been made from templates generated with primers directed against this gene. (Marr et al., 2006) The proteins encoded by Tbp, Taf1, Taf2 and Taf11 are likely peripheral subunits of TFIID. (Wright et al., 2006) The mechanism of transcriptional repression by eve protein involves a direct interaction with the Tbp protein. (Li and Manley, 1998) Hsf interacts directly with the general transcription factor Tbp and these two factor bind cooperatively to heat shock promoters. Interaction of Hsf and Tbp is mediated by residues in both the amino and carboxy terminus of Hsf. The acidic domain of RpII215 associates with Tbp in vitro and is specifically displaced from Tbp upon addition of Hsf. Trl also interacts with Hsf to further stabilise Hsf binding to heat shock elements (HSEs). (Mason and Lis, 1997) Taf105 is physically associated with Tbp in vitro and in vivo. Mutation at position 332 of Tbp disrupts the ability of Taf105 to associated with Tbp. (Vilalta et al., 1997) Mutation at position 332 of Tbp renders it inactive for tRNA gene transcription in vitro. (Vilalta et al., 1997) TfIID binds to a conserved downstream basal promoter element (DPE) that is present in many TATA-box deficient promoters. The DPE acts in conjunction with the initiation site sequence to provide a binding site for TfIID in the absence of a TATA box to mediate transcription of TAT-less promoters. (Burke and Kadonaga, 1996) Tbp can be central to both activation and repression mechanisms. (Manley et al., 1996) In Schneider S-2 cells Tbp is limiting for the expression of both TATA-containing and TATA-lacking RNA polymerase III promoters. (Trivedi et al., 1996) The tandem Adh promoters are differentially transcribed in the embryo owing to critical differences in the core promoter elements. Reconstituted differential Adh promoter transcription in vitro provides evidence that selective Adh promoter utilization is mediated by a specific Tbp-TAF complex in combination with TfIIA. TAFs in the Tbp complex are required for discrimination between the Adh distal and proximal initiator elements. (Hansen and Tjian, 1995) Tbp and TfIIB genes share common features in the promoter regions. (Lira-De Vito et al., 1995) A quadruple complex containing Tbp, Taf1, Taf4 and Taf6 mediates transcriptional synergism by bcd and hb, whereas triple Tbp-Taf complexes lacking one or other coactivator failed to support synergistic activation. The concerted action of multiple regulators with different coactivators helps to establish the pattern and level of segmentation gene transcription during development. (Sauer et al., 1995) eve efficiently blocks, or "squelches", Tbp-enhanced transcription in cotransfected Schneider L2 cells. Squelching does not require eve DNA-binding sites on the reporter plasmids used, but is dependent on the presence of the eve repression domain. (Um et al., 1995) The assembly and purification of various functional Tbp TAF complexes demonstrate that Tbp plus the TAFs is sufficient to replace endogenous TFIID to support activated transcription in vitro. (Chen et al., 1994) The role of the two essential transcription components, TfIIIB and TfIIIC, in response to TPA treatment is studied; only TfIIIB activity is increased. Tbp activity is also increased. Results suggest that the induction of transcription is due to an increase in the activity of TfIIIB which results in an increase in the number of functional transcription complexes. (Garber et al., 1994) TFIID subunit proteins and the Tbp protein can interact in pairwise combinations with several subunits in a network of interactions within TFIID. (Kokubo et al., 1994) Taf1 contains at least two sites of interaction with Tbp. N terminal sites mediate strong physical interaction with Tbp and inhibit Tbp function. Interaction of the N terminal region with Tbp may directly control TATA-box binding. (Kokubo et al., 1994) Recombinant purified TfIIA-L and TfIIA-S can form a stable complex with Tbp on DNA, the recombinant protein can stimulate basal transcription and enhances transcriptional activation. (Yokomori et al., 1994) Purified Taf5 is unable to bind Tbp directly or to interact strongly with the C-terminal domain of Taf1, but can only interact with a complex containing Tbp, Taf1, Taf4 and Taf6. (Dynlacht et al., 1993) Mutations downstream of the TATA element of the Hsp70 promoter reduce the binding of the Tbp gene product. (Emanuel and Gilmour, 1993) Purified TfIID, containing endogenous Tbp and at least seven TAFs is necessary and sufficient for Sp1 activation in a reconstituted transcription reaction. (Hoey et al., 1993) In vitro expressed Taf4 protein interacts directly with Taf1 which itself interacts with Tbp, a complex of all three is suggested. Binding study with various mutants of Taf1 suggests that both Taf4 and Tbp interact with the N-terminal 352-amino acid portion of Taf1. (Kokubo et al., 1993) A number of polypeptides (encoded by Taf1, Taf4, Taf5, Taf6, e(y)1 and Taf12) that are tightly associated with Tbp and are native TFIID components have been purified. Protein blotting experiments suggest that one of these proteins, Taf1, interacts directly with Tbp, while the association between Tbp and the other proteins is either weak or is an indirect association via Taf1. (Kokubo et al., 1993) Taf1 gene product has been isolated and characterised and has been shown to interact directly with Tbp. (Kokubo et al., 1993) TATA complex formation on the Hsp70Bb core promoter shows sequence dependence at the TATA element, at the transcription start site and further downstream. The TATA complex contains Tbp. (Purnell and Gilmour, 1993) See also Dynlacht, Cell 66:563. (Weinzierl et al., 1993) TfIID transcription factor is a multiprotein complex including TATA-binding protein (Tbp) and Tbp-associated factors (TAFs). Taf1 has been partially cloned and the assembly of a partial complex containing recombinant Tbp, Taf1 and Taf4 analysed. This triple complex supports activation of HeLa cell Sp1 and reveals specific interactions between Tbp, Taf1 and Taf4. (Weinzierl et al., 1993) There is a weak but reproducible interaction between the products of the Tbp and Taf6 loci in vitro. Tbp, Taf1, Taf4 and Taf6 products can assemble into a mini-complex in vitro, showing that Taf6 and Taf4 occupy distinct binding sites on the C terminal part of Taf1. (Weinzierl et al., 1993) snRNA:U1:95Ca transcription in vitro requires Tbp. (Zamrod et al., 1993) Overexpression of full length protein in cultured cells can enhance expression dramatically from a weak minimal TATA-containing promoter. (Colgan and Manley, 1992) A TATA-dependent protein-DNA complex is found in fractionated embryonic nuclear extracts. DNase I footprint analysis identifies polypeptides (including Taf12, Tbp and RpII140) that require the Hsp70Bb TATA element for binding to regions of the Hsp70Bb promoter. (Gilmour et al., 1990)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079081 RefSeq (Proteins) • NP_523805 DDBJ / EMBL / GenBank • AAA28926 AAA28931 AAA68629 AAA79092 AAF46754 AAF46754.1 AAL39808 AI512297 AW943695 AY069663 M38082 M38388 U11718 U35147 Entrez Gene - A searchable database of RefSeq genes. • 37476 UniProtKB/Swiss-Prot • P20227
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • TATA-box binding protein (IPR000814) Transcription factor TFIID, C-terminal/DNA glycosylase, N-terminal (IPR012294) Beta2-adaptin/TATA-box binding, C-terminal (IPR012295)
Linkouts
	BioGRID - A database of protein and genetic interactions • 63101 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0003687 DroID - A comprehensive database of gene and protein interactions. • FBgn0003687 DRSC - Results from RNAi screens. • FBgn0003687 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003687 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9874-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003687 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37476 InParanoid A subset of ortholog calls from InParanoid. • AAEL012330-PA Aedes aegypti (mosquito) AGAP010958-PA Anopheles gambiae (mosquito) XP_623088.1 Apis mellifera (honey bee) XP_001949590.1 Acyrthosiphon pisum (pea aphid) At3g13445.1 Arabidopsis thaliana (wall cress) 90475 Batrachochytrium dendrobatidis (chytrid fungus) 121529 Branchiostoma floridae (lancelet) BGIBMGA010619-PA Bombyx mori (silk worm) ENSBTAP00000010109 Bos taurus (bovine) CAL0001658 Candida albicans (a form of yeast) CN12208 Caenorhabditis brenneri (small nematode) CBP02248 Caenorhabditis briggsae (nematode) CE01408 Caenorhabditis elegans (worm) ENSCAFP00000006103 Canis familiaris (dog) CAGL0I06688g Candida glabrata (haploid yeast) Chro.80238 Cryptosporidium hominis (obligate parasite) ENSCINP00000005464 Ciona intestinalis (sea squirt) JA18543 Caenorhabditis japonica (nematode) CMF095C Cyanidioschyzon merolae (red alga) cn12106 Cryptococcus neoformans (yeast-like fungus) cgd8_2030 Cryptosporidium parvum (protozoan parasite) CPIJ007600 Culex pipens (mosquito) CPIJ018368 Culex pipens (mosquito) ENSCPOP00000001072 Cavia porcellus (guinea pig) 100267 Chlamidomonas reinhardtii (green alga) RP20018 Caenorhabditis remanei (nematode) ENSCSAVP00000012157 Ciona savignyi (sedentary tunicate) 209597 Capitella spl (polychaete worm) DDB0185066 Dictyostelium discoideum (amoeba) DEHA2G11836g Debaryomyces Hansenii (yeast) 194512 Daphnia pulex (daphnia) ENSDARP00000010211 Danio rerio (zebra fish) ENSECAP00000006680 Equus caballus (horse) ENSGACP00000021304 Gymnopilus aculeatus (mushroom) ENSGALP00000036926 Gallus gallus (chicken) 86082 Hypsipyla robusta (cedar shoot borer) ENSP00000375942 Homo sapiens (human) ISCW019416-PA Ixodes scapularis (deer tick) KLLA0E10099g Kluyveromyces lactis (yeast) LmjF19.1390 Leishmania major (virus) ENSMODP00000006780 Monodelphis domestica (gray opossum) ENSMMUP00000020642 Macaca mulatta (rhesus monkey) ENSMUSP00000114007 Mus musculus (house mouse) 203375 Nematostella vectensis (starlet anemone) XP_001603648.1 Nasonia vitripennis (wasp) ENSOANP00000020337 Ornithorynchus anatinus (platypus) ENSORLP00000013489 Oryzias latipes (japanese killifish) LOC_Os10g29660.1 Oryza sativa (asian rice) 29198 Ostreococcus tauri (green alga) PHUM010718-PA Pediculus humanus (human body louse) 115252 Physcomitrella patens (moss) ENSPPYP00000019257 Pongo pygmaeus (orangutan) 84970 Phytophthora ramorum (sudden oak death pathogen) 129327 Phytophthora sojae (root rot pathogen) 730014 Populus trichocarpa (black cottonwood) ENSPTRP00000032146 Pan troglodytes (chimpanzee) ENSRNOP00000002038 Rattus norvegicus (norway rat) 4774638 Sorghum bicolor (sorghum) YER148W Saccharomyces cerevisiae (budding yeast) Smp_098950 Schistosoma mansoni (trematode parasite) SPAC29E6.08 Schizosaccharomyces pombe (fission yeast) NP_999786.1 Strongylocentrotus purpuratus (purple sea urchin) XP_795391.2 Strongylocentrotus purpuratus (purple sea urchin) 50358 Trichoplax adhaerens (metazoa) XP_969326.1 Tribolium castaneum (red flour beetle) Tc00.1047053503809.149 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000002633 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000017711 Takifugu rubripes (pufferfish Takifugu) 3812.m02620 Tetrahymena thermophila (ciliate protozoa) 89741.m00028 Trichomonas vaginalis (parasitic flagellated protozoan) 90019.m00264 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000018725 Xenopus tropicalis (frog) YALI0B23056g Yarrowia lipolytica (nonconventional yeast) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/tbp.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37476 modMine - Data generated by the modENCODE project. • FBgn0003687 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • LH13241 ISCW019416 FBgn0231340 FBgn0212662 FBgn0202120 FBgn0041651 FBgn0196487 FBgn0170612 JGI_V11_194512 FBgn0082072 FBgn0148880 FBgn0141682 FBgn0130578 FBgn0112940 FBgn0088667 CPIJ007600 CPIJ018368 BGIBMGA010619 ACYPI005546 ACYPI001633 GB17946 AGAP010958 AAEL012330 PB20491 PHUM533080 GLEAN_08403
Synonyms & Secondary IDs ( 18 )
Reported As
Symbol Synonym	CG9874 (Aoyagi and Wassarman, 2000, Keleman et al., 2009.8.5) dTBP (Georgieva et al., 2001, Muratoglu et al., 2003, Park et al., 2001, Das and Scovell, 2001, Adamkewicz et al., 2001, Park et al., 2001, Pham et al., 1999, Li and Manley, 1999, Bell and Tora, 1999, Li and Manley, 1998, Chen and Tjian, 1996, George et al., 1995, Yokomori et al., 1993, Zamrod et al., 1993, Pugh and Tjian, 1991, Pereira et al., 2001, Ren et al., 2011) dTbp (Farmer et al., 1996, Frank et al., 1995) dtbp (Ren et al., 2011) dTFIID (Pugh and Tjian, 1990, Hoey et al., 1990) RBP (Mueller and Tora, 2004) Tbp (Breiling et al., 2004, Straub et al., 2005, Ren et al., 2011) TBP (Huang et al., 2002, Lebedeva, 2005, Lebedeva et al., 2005, Kim et al., 2004, Ma, 2005, Dantonel et al., 1999, Hochheimer and Tjian, 2003, Hernandez-Hernandez and Ferrus, 2001, Pham and Sauer, 2000, Takada et al., 2000, Georgieva et al., 2000, Burke, 1994.6.30, Baek, 1995.8.30, Breiling et al., 1999, Wang and Lindquist, 1998, Zhou et al., 1998, Sandaltzopoulos and Becker, 1998, Sauer and Tjian, 1997, Kornberg, 1996, Gupta et al., 1996, ten Harmsel and Biggin, 1995, Um et al., 1995, Austin and Biggin, 1995, Sauer et al., 1995, Lira-De Vito et al., 1995, Goodrich and Tjian, 1994, Weinzierl et al., 1993, Sanchez-Elsner et al., 2006, Maile et al., 2004, Marr et al., 2006, Wright et al., 2006, Kim and Lis, 2005, Papp and Muller, 2006, Kahn et al., 2006, Aguilar-Fuentes et al., 2008, Jin et al., 2004, Fu and Ma, 2005, Wang and Brock, 2003, Leibovitch et al., 2002, Wood et al., 2008, Levine and Tjian, 2003, Wang et al., 2007, Isogai et al., 2007, Kopytova et al., 2006, Katzenberger et al., 2006, Metcalf and Wassarman, 2007, Barakat and Stumph, 2008, Aguilar-Fuentes et al., 2006, Wright and Tjian, 2009, Choi et al., 2004, Hsu et al., 2008, Fregoso et al., 2007, Plata et al., 2009, Weake et al., 2009, Aoyagi and Wassarman, 2001, Filion et al., 2010, Park et al., 2010, Li and Arnosti, 2011, Vorobyeva et al., 2011, Santoso and Kadonaga, 2006, Theisen et al., 2010, Vorobyeva et al., 2009) TBP38 (Tansey and Herr, 1997) TFIID (Butler and Kadonaga, 2002, Hochheimer et al., 2002, Gu et al., 2002, Stathopoulos and Levine, 2002, Mizuguchi et al., 2001, Okonogi et al., 2000, Kal et al., 2000, Leibovitch et al., 2000, Smale et al., 1998, Burke and Kadonaga, 1997, Weber et al., 1997, Kingston et al., 1996, ten Harmsel and Biggin, 1995, Shopland et al., 1995, Weber and Gilmour, 1995, Hansen and Tjian, 1995, Chen et al., 1994, Colgan and Manley, 1992, Muhich et al., 1990, Wampler et al., 1990, Lewin, 1990, ) TfIID (Smale, 1997) TFIIDτ (Kokubo et al., 1993, Kokubo et al., 1993, Kokubo et al., 1993, Kokubo et al., 1993)
Name Synonym	42kDa polypeptide (Gilmour et al., 1990) TATA-binding protein (Breiling et al., 2004, Kim and Lis, 2005) TATA binding protein (Shalaby et al., 2009) TATA box-binding protein (Wright et al., 2006, Barakat and Stumph, 2008, Li and Arnosti, 2011) TATA-box binding protein (Metcalf and Wassarman, 2007) TATA box binding protein (Choi et al., 2004)
Secondary FlyBase IDs
	FBgn0013961 FBpp0071596 FBtr0071679
References ( 196 )
Generate a list of	All references relating to this gene ( 196 )
List References by type	Research paper  ( 135 ) Supplementary material  ( 5 ) Review  ( 35 ) Personal communication to FlyBase  ( 3 ) Abstract  ( 7 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 3 ) Letter  ( 3 ) Protein sequence record  ( 1 ) Computer file  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 6 )
	Li and Arnosti, 2011, Curr. Biol. 21(5): 406--412 Long- and short-range transcriptional repressors induce distinct chromatin States on repressed genes. [FBrf0213170] Ren et al., 2011, Hum. Mol. Genet. 20(17): 3424--3436 A Drosophila model of the neurodegenerative disease SCA17 reveals a role of RBP-J/Su(H) in modulating the pathological outcome. [FBrf0214639] Vorobyeva et al., 2011, Cell Cycle 10(11): 1821--1827 SAYP interacts with DHR3 nuclear receptor and participates in ecdysone-dependent transcription regulation. [FBrf0213948] Filion et al., 2010, Cell 143(2): 212--224 Systematic protein location mapping reveals five principal chromatin types in Drosophila cells. [FBrf0212051] Park et al., 2010, Biochim. biophys. Acta 1799(7): 510--519 Regulation of the Drosophila p38b gene by transcription factor DREF in the adult midgut. [FBrf0211129] Theisen et al., 2010, Mol. Cell. Biol. 30(14): 3471--3479 Three key subregions contribute to the function of the downstream RNA polymerase II core promoter. [FBrf0214907] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...