anch, ana, anarchist
Gene model reviewed during 5.50
Interacts with pim and Sse. Cleavage of thr contributes to inactivation of Sse.
Proteolytically cleaved after the metaphase-to-anaphase transition, C-terminal cleavage product is degraded. Cleavage can only proceed within complexes that contain active Sse.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\thr using the Feature Mapper tool.
In Northern blots, thr transcript is detected at high levels in 4 hr and younger embryos, and at lower levels throughout the rest of embryogenesis. In situ hybridization experiments show thr transcript to be evenly distributed in the embryo before cellularization. After cellularization, thr transcript continues to be expressed ubiquitously, but is at higher levels in the pole cell region. Expression declines during germ band extension. After germ band extension, thr transcript is detected in the central and peripheral nervous sys ems. After germ band retraction, a subset of cells in the ventral nerve cord and the proliferating areas of the brain express thr. RNAse protection experiments show that thr transcript is present in third instar larvae, in adults of both sexes, and in Schneider line 2 cells.
GBrowse - Visual display of RNA-Seq signalsView Dmel\thr in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly long spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
The thr gene was cloned by a combination of chromosome microdissection and P element tagging: predicted protein bears little resemblence to any sequence previously reported. Most significant similarity is over a 26 amino acid stretch where similarity to the S.pombe nuc2 nuclear scaffold-like protein is detectable. Mutants in thr show abnormal mitoses, becoming delayed at mitosis 15, with the chromosomes remaining at the metaphase plate, and subsequently decondensing. Maternal mRNA and protein are apparently sufficient for 14 rounds of mitosis in thr mutant embryos.