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Gene Dmel\tipE

General Information
Symbol	Dmel\tipE	Species	D. melanogaster
Name	temperature-induced paralytic E	Annotation symbol	CG1232
Feature type	protein_coding_gene	FlyBase ID	FBgn0003710
Gene Model Status	Current	Stock availability	6 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map	3-13.5
Cytogenetic map	64A10-64A10	Sequence location	3L:4,188,530..4,193,788 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene temperature-induced paralytic E is referred to in FlyBase by the symbol Dmel\tipE (CG1232, FBgn0003710). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: sodium channel regulator activity. There is experimental evidence that it is involved in the biological process: male courtship behavior, veined wing generated song production; cellular response to heat; positive regulation of sodium ion transport via voltage-gated sodium channel activity. 16 alleles are reported. The phenotype of these alleles is annotated with: trichogen cell. It has 2 annotated transcripts and 2 annotated polypeptides. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, during late pupal stages, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system. Gene sequence location is 3L:4188530..4193788.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	tipE (J.C. Hall) Mutant becomes paralyzed rapidly at 38; recovers within seconds when temperature is lowered. No apparent increase in recovery times with longer exposure to high temperature (Jackson et al., 1986). When in a double mutant combination with parats1, tipE results in action potential failures in recordings from larval motor neurons at 33, a temperature at which either parats1 or tipE alone seems to have normal nerve conduction (Ganetzky, 1986). Adult action potential failures in napts;tipE double mutants occur at a lower temperature (31) than in wild type (Ganetzky, 1986); flies of this double-mutant genotype exhibit poorer viability and become paralyzed at lower temperatures than either mutant alone. The parats1; tipE double mutants also survive poorly, showing unconditional lethality in combinations with certain para alleles; In para/+; tipE/tipE mutants, para alleles become semi-dominant for heat-induced paralysis (Ganetzky, 1986). Biochemically, tipE leads to reduced levels of saxitonin binding activity (not temperature-dependent) in adult head extracts, but the dissociation constants for such binding is normal (Jackson et al., 1986). The mutant is slightly resistant to veratrine and shows enhanced tetrodotoxin sensitivity (Jackson et al., 1986). Sodium current density in cultured embryonic neurons is about half that recorded using "tight-seal" techniques from wild-type cells (O'Dowd and Aldrich, 1988, J. Neurosci. 8: 3633-43). When rather lengthy heat treatments are given to seits, the fairly long recovery times are partially suppressed by tipE. Partial suppression of Sh-induced leg shaking is also effected by tipE (Jackson et al., 1986).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	Sequence features BKN31194 HFA08738 BKN46061
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 64A10-64A10   Limits computationally determined from genome sequence between P{PZ}l(3)rG166rG166 and P{PZ}sinu06524
Experimentally Determined Cytological Location
Cytogenetic map Notes References 64B2-64B2     (Hall, 1995.5.24)
Experimentally Determined Recombination Data
Location	3-13.5
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\tipE for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Multiphase exon postulated: this gene shares a region of coding sequence with an overlapping gene, but different reading frames are utilized in the overlapping coding region.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) tipE-RA FBtr0073219  NM_079196   4053   452 tipE-RB FBtr0073220  NM_168067   3907   452
Additional Transcript Data & Comments
Reported size (kB)	5.4, 4.4, 1.7 (northern blot) (Feng et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein tipE-PA   FBpp0073075   50.2   452   4.10     NP_523920   AAF47857 tipE-PB   FBpp0073076   50.2   452   4.10     NP_728952   AAN11591
Additional Polypeptide Data & Comments
Reported size (kDa)	452 (aa); 50 (kD predicted) (Feng et al., 1995)
Comments	tipE was shown to be a highly glycosylated integral membrane protein. It dramatically stimulates functional expression of the sodium channel alpha subunit encoded by para in Xenopus oocytes. (Feng et al., 1995)
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF47857   AE014296 AAN11591   AI387939   AW941827   AX093915   AY060317 AAL25356   BI169358   BI356446   BI367107   CK133615   CO266776   CO267805   CO274388   CO275940   CO308902   CO310450   CO331558   CO332743   CO340965   CZ471629   CZ474120   EC064683   EC079156   EC195066   EC202414   EC215110   EC216625   EC221976   EC240660   EC245109   EC249471   EC254683   EC260172   GH739357   GH739802   GH740856   GH741661   GH743067   GH743635   GH744154   GH744651   GH745637   GH746291   GH746507   GH761481   GH761779   GH763815   GH763959   GH765084   GH766140   GH766257   GH766726   GH768155   U27561 AAC48307   Z83485
UniProtKB/Swiss-Prot	P48613
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   presumptive embryonic/larval peripheral nervous system   (Feng et al., 1995) central nervous system   (Feng et al., 1995) antenno-maxillary complex   (Feng et al., 1995) northern blot Stage Tissue/Position (including subcellular localization) Reference adult stage   adult head   (Feng et al., 1995) antenna   (Feng et al., 1995) leg   (Feng et al., 1995)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0003710    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0003710 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, during late pupal stages, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0003710 expression level Developmental Stage   Expression Level embryo 00-02hr    11 embryo 02-04hr    27 embryo 04-06hr    20 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    58 embryo 12-14hr    117 embryo 14-16hr    291 embryo 16-18hr    563 embryo 18-20hr    630 embryo 20-22hr    569 embryo 22-24hr    703 larva L1    645 larva L2    224 larva L3 12hr old    67 larva L3 puffstage 1-2    47 larva L3 puffstage 3-6    101 larva L3 puffstage 7-9    124 white prepupae new    100 white prepupae 12hr    195 white prepupae 24hr    254 pupae 2d postWPP    463 pupae 3d postWPP    832 pupae 4d postWPP    463 adult male 01day    464 adult male 05day    454 adult male 30day    478 adult female 01day    223 adult female 05day    69 adult female 30day    72 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    11 embryo 02-04hr    27 embryo 04-06hr    20 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    58 embryo 12-14hr  (117) embryo 14-16hr  (291) embryo 16-18hr  (563) embryo 18-20hr  (630) embryo 20-22hr  (569) embryo 22-24hr  (703) larva L1  (645) larva L2  (224) larva L3 12hr old    67 larva L3 puffstage 1-2    47 larva L3 puffstage 3-6  101 larva L3 puffstage 7-9  (124) white prepupae new    100 white prepupae 12hr  (195) white prepupae 24hr  (254) pupae 2d postWPP  (463) pupae 3d postWPP  (832) pupae 4d postWPP  (463) adult male 01day  (464) adult male 05day  (454) adult male 30day  (478) adult female 01day  (223) adult female 05day    69 adult female 30day    72 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    11 embryo 02-04hr    27 embryo 04-06hr    20 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    58 embryo 12-14hr    117 embryo 14-16hr    291 embryo 16-18hr    563 embryo 18-20hr    630 embryo 20-22hr    569 embryo 22-24hr    703 larva L1    645 larva L2    224 larva L3 12hr old    67 larva L3 puffstage 1-2    47 larva L3 puffstage 3-6    101 larva L3 puffstage 7-9    124 white prepupae new    100 white prepupae 12hr    195 white prepupae 24hr    254 pupae 2d postWPP    463 pupae 3d postWPP    832 pupae 4d postWPP    463 adult male 01day    464 adult male 05day    454 adult male 30day    478 adult female 01day    223 adult female 05day    69 adult female 30day    72 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    11 embryo 02-04hr    27 embryo 04-06hr    20 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    58 embryo 12-14hr    117 embryo 14-16hr    291 embryo 16-18hr    563 embryo 18-20hr    630 embryo 20-22hr    569 embryo 22-24hr    703 larva L1    645 larva L2    224 larva L3 12hr old    67 larva L3 puffstage 1-2    47 larva L3 puffstage 3-6    101 larva L3 puffstage 7-9    124 white prepupae new    100 white prepupae 12hr    195 white prepupae 24hr    254 pupae 2d postWPP    463 pupae 3d postWPP    832 pupae 4d postWPP    463 adult male 01day    464 adult male 05day    454 adult male 30day    478 adult female 01day    223 adult female 05day    69 adult female 30day    72 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    11 embryo 02-04hr    27 embryo 04-06hr    20 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    58 embryo 12-14hr    117 embryo 14-16hr    291 embryo 16-18hr    563 embryo 18-20hr    630 embryo 20-22hr    569 embryo 22-24hr    703 larva L1    645 larva L2    224 larva L3 12hr old    67 larva L3 puffstage 1-2    47 larva L3 puffstage 3-6    101 larva L3 puffstage 7-9    124 white prepupae new    100 white prepupae 12hr    195 white prepupae 24hr    254 pupae 2d postWPP    463 pupae 3d postWPP    832 pupae 4d postWPP    463 adult male 01day    464 adult male 05day    454 adult male 30day    478 adult female 01day    223 adult female 05day    69 adult female 30day    72 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0003710 expression level Developmental Stage   Expression Level embryo 00-02hr    11 embryo 02-04hr    27 embryo 04-06hr    20 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    58 embryo 12-14hr    117 embryo 14-16hr    291 embryo 16-18hr    563 embryo 18-20hr    630 embryo 20-22hr    569 embryo 22-24hr    703 larva L1    645 larva L2    224 larva L3 12hr old    67 larva L3 puffstage 1-2    47 larva L3 puffstage 3-6    101 larva L3 puffstage 7-9    124 white prepupae new    100 white prepupae 12hr    195 white prepupae 24hr    254 pupae 2d postWPP    463 pupae 3d postWPP    832 pupae 4d postWPP    463 adult male 01day    464 adult male 05day    454 adult male 30day    478 adult female 01day    223 adult female 05day    69 adult female 30day    72 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    11 embryo 02-04hr    27 embryo 04-06hr    20 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    58 embryo 12-14hr  117 embryo 14-16hr  (291) embryo 16-18hr  (563) embryo 18-20hr  (630) embryo 20-22hr  (569) embryo 22-24hr  (703) larva L1  (645) larva L2  (224) larva L3 12hr old    67 larva L3 puffstage 1-2    47 larva L3 puffstage 3-6  101 larva L3 puffstage 7-9  124 white prepupae new    100 white prepupae 12hr  (195) white prepupae 24hr  (254) pupae 2d postWPP  (463) pupae 3d postWPP  (832) pupae 4d postWPP  (463) adult male 01day  (464) adult male 05day  (454) adult male 30day  (478) adult female 01day  (223) adult female 05day    69 adult female 30day    72 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    11 embryo 02-04hr    27 embryo 04-06hr    20 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    58 embryo 12-14hr    117 embryo 14-16hr    291 embryo 16-18hr    563 embryo 18-20hr    630 embryo 20-22hr    569 embryo 22-24hr    703 larva L1    645 larva L2    224 larva L3 12hr old    67 larva L3 puffstage 1-2    47 larva L3 puffstage 3-6    101 larva L3 puffstage 7-9    124 white prepupae new    100 white prepupae 12hr    195 white prepupae 24hr    254 pupae 2d postWPP    463 pupae 3d postWPP    832 pupae 4d postWPP    463 adult male 01day    464 adult male 05day    454 adult male 30day    478 adult female 01day    223 adult female 05day    69 adult female 30day    72 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    11 embryo 02-04hr    27 embryo 04-06hr    20 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    58 embryo 12-14hr    117 embryo 14-16hr    291 embryo 16-18hr    563 embryo 18-20hr    630 embryo 20-22hr    569 embryo 22-24hr    703 larva L1    645 larva L2    224 larva L3 12hr old    67 larva L3 puffstage 1-2    47 larva L3 puffstage 3-6    101 larva L3 puffstage 7-9    124 white prepupae new    100 white prepupae 12hr    195 white prepupae 24hr    254 pupae 2d postWPP    463 pupae 3d postWPP    832 pupae 4d postWPP    463 adult male 01day    464 adult male 05day    454 adult male 30day    478 adult female 01day    223 adult female 05day    69 adult female 30day    72 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    11 embryo 02-04hr    27 embryo 04-06hr    20 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    58 embryo 12-14hr    117 embryo 14-16hr    291 embryo 16-18hr    563 embryo 18-20hr    630 embryo 20-22hr    569 embryo 22-24hr    703 larva L1    645 larva L2    224 larva L3 12hr old    67 larva L3 puffstage 1-2    47 larva L3 puffstage 3-6    101 larva L3 puffstage 7-9    124 white prepupae new    100 white prepupae 12hr    195 white prepupae 24hr    254 pupae 2d postWPP    463 pupae 3d postWPP    832 pupae 4d postWPP    463 adult male 01day    464 adult male 05day    454 adult male 30day    478 adult female 01day    223 adult female 05day    69 adult female 30day    72 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0003710    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0003710 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis no informative data Adult Male Accessory Gland     Adult Carcass no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_14
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003710 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG18675-RA;CG1232-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for tipE Stages(s) 4-6 Stages(s) 7-8 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 tipEGD2293 Other Phenotypes Allele chemical resistant tipE1 courtship behavior defective | male | recessive tipE1 neuroanatomy defective | recessive | heat sensitive tipEunspecified paralytic | heat sensitive tipE1 tipEunspecified paralytic | heat sensitive (with tipE1) tipETE2 paralytic | heat sensitive (with tipETE2) tipE1 paralytic | recessive | heat sensitive tipEunspecified visible, with Scer\GAL4pnr-MD237 tipEGD2293 wild-type tipE+t7.4 tipE+t12 tipE+t42 Phenotype manifest in Allele neuromuscular junction & synapse tipEunspecified synapse | ectopic | heat sensitive tipEunspecified trichogen cell, with Scer\GAL4pnr-MD237 tipEGD2293
Classical Alleles ( 7 )
For All Classical Alleles Show
Allele of tipE Class Mutagen Stocks Known lesion tipEd00490 P-element activity 1 -- tipENP6572 1 -- tipENP6627 1 -- tipE1 loss of function allele ethyl methanesulfonate 0 Yes tipEd04767 P-element activity 0 -- tipETE2 gamma ray 0 Yes tipEunspecified 0 --
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of tipE Class Mutagen Stocks Known lesion tipEGD2293 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes tipEJF02148 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tipE+t12 in vitro construct - genomic fragment 0 Yes tipE+t42 in vitro construct - genomic fragment 0 Yes tipE+t7.4 in vitro construct - genomic fragment 0 Yes tipEhs.PF in vitro construct - regulatory fusion 0 Yes tipEmut.hs in vitro construct - site directed mutagenesis 0 Yes tipEtFa in vitro construct - genomic fragment 0 Yes tipEtFb in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)9-7K (Kulkarni et al., 1994) Df(3L)A466 (Kulkarni et al., 1994) Df(3L)Exel6100 (Ryder, 2004.4.26) Df(3L)GN19 (Feng et al., 1995) Df(3L)HR277 (Feng et al., 1995) Df(3L)TE1 (Kulkarni et al., 1994, Feng et al., 1995) Df(3L)TE3 (Kulkarni et al., 1994, Feng et al., 1995) Df(3L)X37 (Feng et al., 1995) T(2;3)TE2 (Feng et al., 1995)
Not disrupted in	Df(3L)ems13 (Feng et al., 1995) Df(3L)GN34 (Feng et al., 1995) Df(3L)HR298 (Feng et al., 1995) T(Y;3)B887 (Kulkarni et al., 1994)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data heat-shock construct P{hs-tipE} NA P{mut-tipE} NA UAS construct P{GD2293} NA P{TRiP.JF02148} NA characterization construct P{tipE+7.4} NA P{tipE+12} NA P{tipE+42} NA P{tipEtFa} NA P{tipEtFb} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap binary system P{GawB}tipENP6572 No P{GawB}tipENP6627 No insertion of mobile activating element P{XP}tipEd00490 NA P{XP}tipEd04767 NA
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function
CV term Evidence References
sodium channel regulator activity inferred from direct assay (Feng et al., 1995)
Biological Process
CV term Evidence References
cellular response to heat inferred from direct assay (Feng et al., 1995) male courtship behavior, veined wing generated song production inferred from mutant phenotype (Peixoto and Hall, 1998) positive regulation of sodium ion transport via voltage-gated sodium channel activity inferred from direct assay (Feng et al., 1995, Derst et al., 2006)
Cellular Component
CV term Evidence References
integral to plasma membrane inferred from direct assay (Feng et al., 1995) plasma membrane inferred from direct assay AND inferred from sequence or structural similarity (Feng et al., 1995)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
voltage-gated sodium channel activity inferred from sequence or structural similarity with EMBL:AF131734 (FlyBase, 1992-) traceable author statement (Littleton and Ganetzky, 2000)
Biological Process
CV term Evidence References
sodium ion transport non-traceable author statement (Littleton and Ganetzky, 2000)
Cellular Component
CV term Evidence References
integral to plasma membrane non-traceable author statement (Swiss-Prot Project Members, 1996.2.1) plasma membrane traceable author statement (Littleton and Ganetzky, 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions tipE allele Gene References unspecified mle (Jackson et al., 1986, Ganetzky, 1986)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0003710
Orthologs
Genome-wide drosophilid orthologs	Dana\GF10561 Dere\GG15201 Dgri\GH15930 Dmoj\GI21413 Dper\GL12833 Dpse\GA11553 Dsec\GM14633 Dsim\GD13821 Dvir\GJ12831 Dwil\GK12714 Dyak\GE21421
Curated drosophilid orthologs	Dpse\GA11553 (Derst et al., 2006, ) Dsec\tipE (Gleason and Ritchie, 2004) Dsim\tipE (Gleason and Ritchie, 2004)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL002127-PA Aedes aegypti (mosquito) AGAP002820-PA Anopheles gambiae (mosquito) BGIBMGA010081-PA Bombyx mori (silk worm) CPIJ004297 Culex pipens (mosquito) 5229 Daphnia pulex (daphnia) PHUM010194-PA Pediculus humanus (human body louse) XP_970009.1 Tribolium castaneum (red flour beetle) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB17409 NV50210 LH13130 FBgn0238681 FBgn0214723 FBgn0200066 FBgn0185519 FBgn0169554 JGI_V11_5229 FBgn0071604 FBgn0150439 FBgn0144143 FBgn0139327 FBgn0123401 FBgn0107454 FBgn0087602 CPIJ004297 BGIBMGA010081 GB12769 AGAP002820 AAEL002127 PHUM488040 GLEAN_01758
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	26249 y1 v1; P{TRiP.JF02148}attP2/TM3, Sb1
Harvard	- P{XP}tipEd00490
Kyoto	105264 y* w*; P{GawB}tipENP6572 / TM6, P{UAS-lacZ.UW23-1}UW23-1 114007 y* w*; P{GawB}tipENP6627 / TM6, P{UAS-lacZ.UW23-1}UW23-1
VDRC	v4482 w1118; P{GD2293}v4482 v4483 w1118; P{GD2293}v4483
Genomic Clones ( 2 )
	BACR39O11 BACR48M07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 46 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH18645
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE10508 RE42593 RE52786
Other clones	36623 38758 99279 103840 149500 156723 214567.3 214573.14 273627 277069 CK01.15A.A6 CK01.33A.A4 EK113953 EK119206 EK147516 EK151861 EK221660 EK259624 EK300525 EK304421 EP11640 RE31965 RP01336 RP001010416 RP001012506 RP001014437 RP001018174 RP001019946 RP001020042 RP001021902 RP001022000 RP001022310 RP001024572 RP001026478 RP001029308 RP001035481 RP001049016 RP001049103 RP001053077 RP001054311 RP001055370 RP001056321
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003710 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38504
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: tipE CG1232 (Gabrielian, 1999.11)
Source for database merge of
Additional comments
Other Comments
	Area matching Drosophila TipE gene, Acc. No. U27561. (Davies, 2001.3.15) Coexpression of para with tipE in Xenopus oocytes results in robust sodium currents. The biophysical and biochemical properties of invertebrate and vertebrate neuronal sodium channels are distinct. (Warmke et al., 1997) tipE defines a novel membrane protein which is essential for modulating para sodium channel functional expression. (Feng et al., 1995) Critical period for tipE expression to alleviate adult mutant paralysis is during pupal development. (Feng et al., 1995) The tipE encoded integral membrane protein dramatically stimulates functional para expression in Xenopus oocytes. tipE has a function in adults that protects them against a heat-induced lethality. (Feng et al., 1995) mlenap-ts1 in combination with tipE1 leads to poor viability at permissive temperature. (Jackson et al., 1986, Ganetzky, 1986)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079196 NM_168067 RefSeq (Proteins) • NP_523920 NP_728952 DDBJ / EMBL / GenBank • AAC48307 AAF47857 AAL25356 AAN11591 AI387939 AW941827 AX093915 AY060317 CZ471629 CZ474120 U27561 Z83485 Entrez Gene - A searchable database of RefSeq genes. • 38504 UniProtKB/Swiss-Prot • P48613
Other Crossreferences
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0003710 DRSC - Results from RNAi screens. • FBgn0003710 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003710 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG18675-RA;CG1232-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003710 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38504 InParanoid A subset of ortholog calls from InParanoid. • AAEL002127-PA Aedes aegypti (mosquito) AGAP002820-PA Anopheles gambiae (mosquito) BGIBMGA010081-PA Bombyx mori (silk worm) CPIJ004297 Culex pipens (mosquito) 5229 Daphnia pulex (daphnia) PHUM010194-PA Pediculus humanus (human body louse) XP_970009.1 Tribolium castaneum (red flour beetle) modMine - Data generated by the modENCODE project. • FBgn0003710 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB17409 NV50210 LH13130 FBgn0238681 FBgn0214723 FBgn0200066 FBgn0185519 FBgn0169554 JGI_V11_5229 FBgn0071604 FBgn0150439 FBgn0144143 FBgn0139327 FBgn0123401 FBgn0107454 FBgn0087602 CPIJ004297 BGIBMGA010081 GB12769 AGAP002820 AAEL002127 PHUM488040 GLEAN_01758
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	CG1232 (Perkins et al., 2009.3.31) TipE (Derst et al., 2006, Shichor et al., 2002, Littleton and Ganetzky, 2000, Zlotkin et al., 1999, Olson et al., 2008, Cohen et al., 2009, He et al., 2011) tip-E (Hall et al., 1991) tipE (Usherwood et al., 2005, Hodges et al., 2002, Usherwood et al., 2007, Feng et al., 1995, Adryan et al., 2007, Repnikova et al., 2010, Burton et al., 2011, Gleason, 2005)
Name Synonym	paralytic-E (Gleason, 2005) temperature-induced- (Gleason, 2005) temperature-induced paralysis locus E (Feng et al., 1995) temperature-induced paralytic E
Secondary FlyBase IDs
References ( 73 )
Generate a list of	All references relating to this gene ( 73 )
List References by type	Research paper  ( 30 ) Supplementary material  ( 2 ) Review  ( 6 ) Personal communication to FlyBase  ( 3 ) Abstract  ( 17 ) FlyBase analysis  ( 2 ) Curated genome annotation  ( 2 ) Patent  ( 1 ) DNA/RNA sequence record  ( 2 ) Protein sequence record  ( 1 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 ) Letter  ( 2 )
Recent research papers ( 3 )
	Burton et al., 2011, Insect Biochem. Mol. Biol. 41(9): 723--732 Differential resistance of insect sodium channels with kdr mutations to deltamethrin, permethrin and DDT. [FBrf0214046] He et al., 2011, PLoS ONE 6(1): e14510 Localization of Receptor Site on Insect Sodium Channel for Depressant β-toxin BmK IT2. [FBrf0212808] Repnikova et al., 2010, J. Neurosci. 30(18): 6466--6476 Sialyltransferase regulates nervous system function in Drosophila. [FBrf0210736] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...