Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tpl using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
JBrowse - Visual display of RNA-Seq signals
View Dmel\Tpl in JBrowse3-47.4
Maps close to Ki.
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Haploinsufficient locus.
Increased dosage of the Is locus suppresses the triplo-lethal, but not the haplo-lethal phenotype of Tpl. Altered dosage of Is in the presence of two copies of Tpl has no obvious effects. Is probably does not directly repress Tpl expression, but rather acts downstream of the Tpl triplo-lethal phenotype.
Lethal when present in either one (Tpl/Df) or three doses (Tpl/Dp) in an otherwise diploid individual. These individuals do not survive to the adult stage, but a few larvae with three doses of 83D-E develop to the third instar. The surviving larvae are also hyperploid for the X chromosome (as in 3X;2A metafemales); they can be produced in genotypes duplicated for 7C and 7D-E.
Three hypotheses are suggested for the lack of point mutations in Tpl : 1) the locus is very small and so the mutation rate is very low, 2) the locus is less sensitive to single base changes than a "typical" structural gene and 3) the locus consists of a gene cluster with at least partial redundancy, so that mutation in one of the genes would not inactivate the entire cluster.
Crosses of Flies with a deficiency for 83D-E in one chromosome 3 and a duplication for the region in the other to wild type fail to produce viable adults.
Flies with a deficiency for 83D-E in one chromosome 3 and a duplication for the region in the other (two doses in all) are viable.
Unique dosage-sensitive locus at 83D-E; First detected by segmental aneuploidy, using the Y-autosome translocations T(Y;3)L132 and T(Y;3)A109.
Record revised by A. Christensen, 19 January 1996.
The Tpl region is both triplo-lethal and haplo-lethal and neither point nor insertional mutations of Tpl have been isolated despite extensive mutagenesis analysis. This raises the possibility that Tpl consists of a cluster of genes with at least partial redundancy. The "Osiris" family of genes contains 23 members. 20 of the "Osiris" genes are located in a cluster within the Tpl region of the genome at 83D-83E (the remaining 3 members - Osi21, Osi22 and Osi23 - are located at three different sites in the genome. None of these 3 loci are triplo-lethal or are located within a haplo-insufficient region). The "Osiris" gene family is also present in the A.gambiae genome and shows remarkable synteny and sequence conservation with the D.melanogaster cluster. There appears to have been selection on the "Osiris" family both for expression level and function. It is suggested that the "Osiris" gene family cluster corresponds to Tpl.
