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Gene Dmel\tsh

General Information
Symbol	Dmel\tsh	Species	D. melanogaster
Name	teashirt	Annotation symbol	CG1374
Feature type	protein_coding_gene	FlyBase ID	FBgn0003866
Gene Model Status	Current	Stock availability	13 publicly available
Also Known As	ae
Genomic Location
Chromosome (arm)	2L	Recombination map	2-55.8
Cytogenetic map	40A5-40A5	Sequence location	2L:21,828,593..21,837,011 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene teashirt is referred to in FlyBase by the symbol Dmel\tsh (CG1374, FBgn0003866). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription. There is experimental evidence for 15 unique biological process terms, many of which group under: single-organism developmental process; system development; segment specification; biological regulation; regulation of RNA metabolic process; trunk segmentation; post-embryonic organ morphogenesis; sensory organ development; proximal/distal pattern formation, imaginal disc; negative regulation of cellular biosynthetic process; renal tubule development; regionalization; embryonic pattern specification; embryonic development via the syncytial blastoderm; digestive tract development. 61 alleles are reported. The phenotypes of these alleles are annotated with: adult segment; organ system subdivision; organ system; thoracic segment; acellular anatomical structure; appendage segment; imaginal precursor; cell cycle; cellular component organization or biogenesis; embryonic gnathal segment; non-connected developing system; larval head segment; external compound sense organ. It has one annotated transcript and one annotated polypeptide. Protein features are: Teashirt; Teashirt family; Zinc finger, C2H2; Zinc finger, C2H2-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval/adult Malpighian tubules, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1626150_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of tsh. Gene sequence location is 2L:21828593..21837011.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	*ae: aeroplane Wings spread, balancers drooping. Overlaps wild type. RK3.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 40A5-40A5   Limits computationally determined from genome sequence between P{lacW}Df31k05815 and P{PZ}cul-202074&P{lacW}l(2)k08110k08110
Experimentally Determined Cytological Location
Cytogenetic map Notes References 40A1-40A4   (determined by in situ hybridization)   (Spradling et al., 1999) 40A1-40A4     (BDGP Project Members, 1994-1999) 40A-40C   (determined by in situ hybridization)   (Hartenstein and Jan, 1992) 40A-40A   (determined by in situ hybridization)   (Fasano et al., 1991)
Experimentally Determined Recombination Data
Location	2-55.8   2-54.6 (Soanes and Bell, 1999)
Left of (cM)	cn (Soanes and Bell, 1999)
Right of (cM)	b (Soanes and Bell, 1999)
Notes	Map position determined using tshae-l. (Soanes and Bell, 1999)
Gene Model & Products
Please see the GBrowse view of Dmel\tsh for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.44 Low-frequency junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) tsh-RA FBtr0085906  NM_078891   4732   954
Additional Transcript Data & Comments
Reported size (kB)	8.5, 8.1, 5.4, 5.0 (northern blot) (Fasano et al., 1991)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein tsh-PA   FBpp0085261   100.6   954   8.64     NP_523615   AAF57236
Additional Polypeptide Data & Comments
Reported size (kDa)	993 (aa); 106 (kD) (Fasano et al., 1991)
Comments	Several isoforms of tsh protein are observed on gels. The smaller 116kD form was determined to be a hypophosphorylated form.The phosphorylated forms have apparent molecular weights of 120~130kD. (Gallet et al., 1999) The tsh protein has three separated Zn fingers of the HX5H type which are also found in the hb, human PRDII-BF1,Pep, and Su(var)3-7 proteins. Outside of the Zn-finger region, no clear regions of similarity were observed to other proteins. (Fasano et al., 1991)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, C2H2-like (IPR015880) Zinc finger, C2H2 (IPR007087) Teashirt (IPR027009) Teashirt family (IPR027008)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA140723   AA140734   AA141256   AE014134 AAF57236   AI294468   AI297086   AI512694   BG637450   BI484200   BI575139   BI577898   BI627693   BI628693   BI630363   BP543345   BP543346   BP544748   BP544749   BP545990   BT015201 AAT94430   BT030762   CO187403   CO189318   CO189321   CO264894   CO265136   CO271729   CO298393   CO299335   CO305641   CO307861   CO321364   CO322258   CO323961   CO324044   CO327519   CO331659   CO343992   EC062194   EC064934   EC071628   EC084239   EC085944   EC085962   EC086210   EC197127   EC203931   EC206321   EC209829   EC217706   EC226187   EC235223   EC238123   EC240335   EC240921   EC249277   EC256248   G00738   M57496 AAA28983
UniProtKB/Swiss-Prot	P22265
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References protein binding site FBsf0000159776 2L:21,853,033..21,853,048 bound_moiety=abd-A-XP evidence=experimental bound_moiety=Antp-XP bound_moiety=Ubx-XP (Bergman et al., 2005, McCormick et al., 1995) protein binding site FBsf0000159777 2L:21,853,539..21,853,547 bound_moiety=ftz-XP evidence=experimental (Core et al., 1997, Bergman et al., 2005) protein binding site FBsf0000159778 2L:21,853,291..21,853,310 bound_moiety=Antp-XP bound_moiety=abd-A-XP evidence=experimental bound_moiety=Ubx-XP (McCormick et al., 1995, Bergman et al., 2005) protein binding site FBsf0000159779 2L:21,853,580..21,853,590 bound_moiety=ftz-XP evidence=experimental (Core et al., 1997, Bergman et al., 2005) protein binding site FBsf0000159780 2L:21,853,215..21,853,235 bound_moiety=abd-A-XP evidence=experimental bound_moiety=Antp-XP bound_moiety=Ubx-XP (McCormick et al., 1995, Bergman et al., 2005) protein binding site FBsf0000159781 2L:21,853,391..21,853,398 bound_moiety=ftz-XP evidence=experimental (Core et al., 1997, Bergman et al., 2005) protein binding site FBsf0000160453 2L:21,853,143..21,853,155 evidence=experimental bound_moiety=Antp-XP bound_moiety=Ubx-XP bound_moiety=abd-A-XP (Bergman et al., 2005, McCormick et al., 1995) protein binding site FBsf0000160454 2L:21,853,488..21,853,515 bound_moiety=ftz-XP evidence=experimental (Core et al., 1997, Bergman et al., 2005) regulatory region FBsf0000435125 2L:21,852,966..21,853,191 evidence=Reporter construct (in vivo) (McCormick et al., 1995, Halfon, 2012.6.19) regulatory region FBsf0000435265 2L:21,851,516..21,853,659 evidence=Reporter construct (in vivo) (McCormick et al., 1995, Halfon, 2012.6.19) regulatory region FBsf0000435632 2L:21,847,519..21,855,735 evidence=Reporter construct (in vivo) (Core et al., 1997, Halfon, 2012.6.19) regulatory region FBsf0000435799 2L:21,852,965..21,853,378 evidence=Reporter construct (in vivo) (McCormick et al., 1995, Halfon, 2012.6.19) regulatory region FBsf0000436293 2L:21,853,101..21,853,428 evidence=Reporter construct (in vivo) (McCormick et al., 1995, Halfon, 2012.6.19) regulatory region FBsf0000436302 2L:21,853,372..21,853,592 evidence=Rescue construct (Core et al., 1997, Halfon, 2012.6.19) regulatory region FBsf0000436573 2L:21,852,966..21,853,428 evidence=Reporter construct (in vivo) (McCormick et al., 1995, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage   head   (Roder et al., 1992) presumptive embryonic/larval central nervous system   (Fasano et al., 1991) midgut constriction 2   (Fasano et al., 1991) embryonic/larval anus   (Fasano et al., 1991) epidermis of parasegment 3 -- parasegment 13   (Fasano et al., 1991) embryonic stage 13   mesothoracic segment   (Roder et al., 1992) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   embryonic/larval pharynx   (Fasano et al., 1991) somatic mesoderm   (Fasano et al., 1991) visceral mesoderm   (Fasano et al., 1991) blastoderm stage   parasegment 3 -- parasegment 13   (Roder et al., 1992) embryonic stage 4 -- 6   anlage in statu nascendi   (Fisher et al., 2012) dorsal ectoderm anlage   (Fisher et al., 2012) organism | gap expression pattern   (Fisher et al., 2012) trunk mesoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi ventral ectoderm anlage   (Fisher et al., 2012) embryonic stage 7 -- 8   amnioserosa anlage   (Fisher et al., 2012) dorsal ectoderm   (Fisher et al., 2012) organism | gap expression pattern   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) embryonic cycle 14A   organism | 40-60% egg length   (Fasano et al., 1991) Comment:reference states 45-60% egg length embryonic stage 9 -- 10   dorsal ectoderm   (Fisher et al., 2012) organism | gap expression pattern   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   dorsal epidermis primordium   (Fisher et al., 2012) ventral epidermis primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13   abdominal segment 1 -- abdominal segment 8   (Roder et al., 1992) embryonic stage 13 -- 16   embryonic dorsal epidermis   (Fisher et al., 2012) embryonic midgut chamber   (Fisher et al., 2012) embryonic ventral epidermis   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Fasano et al., 1991)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage 8   parasegment 3 -- parasegment 14   (Bachiller et al., 1994) immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 10   embryonic thoracic segment   (Pages and Kerridge, 2000) embryonic abdominal segment 1 -- embryonic abdominal segment 8   (Pages and Kerridge, 2000) embryonic stage 11   epidermis of parasegment 3   (McCormick et al., 1995) mesoderm of parasegment 3   (McCormick et al., 1995) epidermis of parasegment 4   (McCormick et al., 1995) mesoderm of parasegment 4   (McCormick et al., 1995) epidermis of parasegment 5   (McCormick et al., 1995) mesoderm of parasegment 5   (McCormick et al., 1995) ventral nerve cord | restricted   (McCormick et al., 1995) Comment:parasegments 3-13 embryonic stage 15   embryonic Malpighian tubule | restricted   (Denholm et al., 2003) second instar larval stage   wing disc   (Wu and Cohen, 2002) third instar larval stage   wing disc   (Wu and Cohen, 2002)
Additional Descriptive Data
	It appears that the hypophosphorylated form of tsh protein is largely cytoplasmic while the phosphorylated forms are nuclear. (Gallet et al., 1999) tsh protein is expressed in the presumptive notum region of the wing disc and repressed in the presumptive wing region (Wu and Cohen, 2002) tsh expression is specific to the trunk region of the developing embryo and is responsive to changes in homeotic gene expression. (Bachiller et al., 1994) Expression of the tsh protein was detected in stage 15 embryonic Malpighian tubule stellate cells. (Denholm et al., 2003)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Bessa et al., 2009, Alexandre et al., 1996)
Expression Deduced from Reporters
Reporter: P{A92}tsh1 Stage Tissue/Position (including subcellular localization) Reference embryonic stage   visceral mesoderm   (Fasano et al., 1991) presumptive embryonic/larval peripheral nervous system   (Fasano et al., 1991) midgut constriction 2   (Fasano et al., 1991) somatic mesoderm   (Fasano et al., 1991) central nervous system   (Fasano et al., 1991) embryonic/larval pharynx   (Fasano et al., 1991) epidermis of parasegment 3 -- parasegment 13   (Fasano et al., 1991) embryonic/larval anus   (Fasano et al., 1991) larval stage   embryonic/larval midgut   (Lemaitre and Coen, 1991) imaginal disc   (Lemaitre and Coen, 1991) embryonic/larval brain   (Lemaitre and Coen, 1991) embryonic/larval cuticle   (Lemaitre and Coen, 1991) wing disc | restricted   (Zecca and Struhl, 2002) wing hinge | presumptive   (Zecca and Struhl, 2002) Reporter: P{GawB}tshc724 Stage Tissue/Position (including subcellular localization) Reference embryonic stage 15   embryonic Malpighian tubule | restricted   (Denholm et al., 2003) larval stage   Malpighian tubule Type II cell   (Sozen et al., 1997) Malpighian tubule Type II cell   (Sozen et al., 1997) adult stage   initial segment of Malpighian tubule   (Sozen et al., 1997) Malpighian tubule Type II cell   (Sozen et al., 1997) Malpighian tubule Type II cell   (Sozen et al., 1997) initial segment of Malpighian tubule | restricted   (Sozen et al., 1997) Reporter: P{GawB}tshGal4-1 Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   peripodial epithelium   (Shiga et al., 1996) wing hinge | presumptive   (Shiga et al., 1996) wing disc | restricted   (Shiga et al., 1996, Tomoyasu et al., 1998) mesothoracic tergum primordium   (Tomoyasu et al., 1998) Reporter: P{lacW}A(2)-6 Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Sun et al., 1995) third instar larval stage   eye disc | restricted   (Sun et al., 1995) wing disc | restricted   (Sun et al., 1995) ventral thoracic disc | restricted   (Sun et al., 1995) antennal disc | restricted   (Sun et al., 1995) Reporter: P{lacW}tshB4-2-12 Stage Tissue/Position (including subcellular localization) Reference embryonic stage | mid   ectoderm of thoracic segment   (Bier et al., 1989) ectoderm of abdominal segment   (Bier et al., 1989) embryonic stage | late   ventral nerve cord   (Bier et al., 1989) embryonic stage 11 -- 17   epidermis of prothoracic segment   (Hartenstein and Jan, 1992) epidermis of abdominal segment 1 -- abdominal segment 9   (Hartenstein and Jan, 1992) epidermis of mesothoracic segment   (Hartenstein and Jan, 1992) embryonic ventral nervous system of mesothoracic segment   (Hartenstein and Jan, 1992) epidermis of metathoracic segment   (Hartenstein and Jan, 1992) embryonic ventral nervous system of prothoracic segment   (Hartenstein and Jan, 1992) embryonic ventral nervous system of metathoracic segment   (Hartenstein and Jan, 1992) abdominal segment 1 -- abdominal segment 9 of embryonic ventral nervous system   (Hartenstein and Jan, 1992) Reporter: P{PZ}tsh04319 Stage Tissue/Position (including subcellular localization) Reference embryonic stage   embryonic Malpighian tubule | restricted   (Denholm et al., 2003) presumptive embryonic/larval central nervous system   (Jackle and Janning, 1998.8.10) embryonic/larval midgut   (Jackle and Janning, 1998.8.10) anterior embryonic/larval midgut   (Jackle and Janning, 1998.8.10) epidermis   (Jackle and Janning, 1998.8.10) excretory star cell   (Denholm et al., 2003) embryonic/larval pharynx   (Jackle and Janning, 1998.8.10) wandering third instar larval stage   imaginal disc   (Meister and Braun, 1995.10) embryonic/larval midgut   (Meister and Braun, 1995.10) Malpighian tubule   (Meister and Braun, 1995.10)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval/adult Malpighian tubules, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    224.775 Larval Midgut    71 Larval Hindgut    14.7 Larval Malpighian Tubules    120.3 Larval Fat Body    36.1 Larval Salivary Gland    9.3 Larval Trachea    14.725 Larval Carcass    189.725 Adult Head    25.1 Adult Eye    30.025 Adult Brain    35.2 Adult Thoracic-Abdominal Ganglion    493.9 Adult Crop    79.1 Adult Midgut    30 Adult Hindgut    13.6 Adult Malpighian Tubules    239.1 Adult Fat Body    25.5 Adult Salivary Gland    12 Adult Heart    45.55 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    28.4 Adult Ovary    9.4 Adult Testis    23.4 Adult Male Accessory Gland    12.8 Adult Carcass    65.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    224.775 Larval Midgut    71 Larval Hindgut    14.7 Larval Malpighian Tubules    120.3 Larval Fat Body    36.1 Larval Salivary Gland    9.3 Larval Trachea    14.725 Larval Carcass    189.725 Adult Head    25.1 Adult Eye    30.025 Adult Brain    35.2 Adult Thoracic-Abdominal Ganglion    493.9 Adult Crop    79.1 Adult Midgut    30 Adult Hindgut    13.6 Adult Malpighian Tubules    239.1 Adult Fat Body    25.5 Adult Salivary Gland    12 Adult Heart    45.55 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    28.4 Adult Ovary    9.4 Adult Testis    23.4 Adult Male Accessory Gland    12.8 Adult Carcass    65.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    224.775 Larval Midgut    71 Larval Hindgut    14.7 Larval Malpighian Tubules    120.3 Larval Fat Body    36.1 Larval Salivary Gland    9.3 Larval Trachea    14.725 Larval Carcass    189.725 Adult Head    25.1 Adult Eye    30.025 Adult Brain    35.2 Adult Thoracic-Abdominal Ganglion    493.9 Adult Crop    79.1 Adult Midgut    30 Adult Hindgut    13.6 Adult Malpighian Tubules    239.1 Adult Fat Body    25.5 Adult Salivary Gland    12 Adult Heart    45.55 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    28.4 Adult Ovary    9.4 Adult Testis    23.4 Adult Male Accessory Gland    12.8 Adult Carcass    65.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    224.775 Larval Midgut    71 Larval Hindgut    14.7 Larval Malpighian Tubules    120.3 Larval Fat Body    36.1 Larval Salivary Gland    9.3 Larval Trachea    14.725 Larval Carcass    189.725 Adult Head    25.1 Adult Eye    30.025 Adult Brain    35.2 Adult Thoracic-Abdominal Ganglion    493.9 Adult Crop    79.1 Adult Midgut    30 Adult Hindgut    13.6 Adult Malpighian Tubules    239.1 Adult Fat Body    25.5 Adult Salivary Gland    12 Adult Heart    45.55 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    28.4 Adult Ovary    9.4 Adult Testis    23.4 Adult Male Accessory Gland    12.8 Adult Carcass    65.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    224.775 Larval Midgut    71 Larval Hindgut    14.7 Larval Malpighian Tubules    120.3 Larval Fat Body    36.1 Larval Salivary Gland    9.3 Larval Trachea    14.725 Larval Carcass    189.725 Adult Head    25.1 Adult Eye    30.025 Adult Brain    35.2 Adult Thoracic-Abdominal Ganglion    493.9 Adult Crop    79.1 Adult Midgut    30 Adult Hindgut    13.6 Adult Malpighian Tubules    239.1 Adult Fat Body    25.5 Adult Salivary Gland    12 Adult Heart    45.55 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    28.4 Adult Ovary    9.4 Adult Testis    23.4 Adult Male Accessory Gland    12.8 Adult Carcass    65.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    224.775 Larval Midgut    71 Larval Hindgut    14.7 Larval Malpighian Tubules    120.3 Larval Fat Body    36.1 Larval Salivary Gland    9.3 Larval Trachea    14.725 Larval Carcass    189.725 Adult Head    25.1 Adult Eye    30.025 Adult Brain    35.2 Adult Thoracic-Abdominal Ganglion    493.9 Adult Crop    79.1 Adult Midgut    30 Adult Hindgut    13.6 Adult Malpighian Tubules    239.1 Adult Fat Body    25.5 Adult Salivary Gland    12 Adult Heart    45.55 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    28.4 Adult Ovary    9.4 Adult Testis    23.4 Adult Male Accessory Gland    12.8 Adult Carcass    65.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    224.775 Larval Midgut    71 Larval Hindgut    14.7 Larval Malpighian Tubules    120.3 Larval Fat Body    36.1 Larval Salivary Gland    9.3 Larval Trachea    14.725 Larval Carcass    189.725 Adult Head    25.1 Adult Eye    30.025 Adult Brain    35.2 Adult Thoracic-Abdominal Ganglion    493.9 Adult Crop    79.1 Adult Midgut    30 Adult Hindgut    13.6 Adult Malpighian Tubules    239.1 Adult Fat Body    25.5 Adult Salivary Gland    12 Adult Heart    45.55 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    28.4 Adult Ovary    9.4 Adult Testis    23.4 Adult Male Accessory Gland    12.8 Adult Carcass    65.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    224.775 Larval Midgut    71 Larval Hindgut    14.7 Larval Malpighian Tubules    120.3 Larval Fat Body    36.1 Larval Salivary Gland    9.3 Larval Trachea    14.725 Larval Carcass    189.725 Adult Head    25.1 Adult Eye    30.025 Adult Brain    35.2 Adult Thoracic-Abdominal Ganglion    493.9 Adult Crop    79.1 Adult Midgut    30 Adult Hindgut    13.6 Adult Malpighian Tubules    239.1 Adult Fat Body    25.5 Adult Salivary Gland    12 Adult Heart    45.55 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    28.4 Adult Ovary    9.4 Adult Testis    23.4 Adult Male Accessory Gland    12.8 Adult Carcass    65.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    no informative data   NA  Eye    30.025   NA  Brain    35.2   224.775  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    493.9   NA  Crop    79.1   71  Midgut    no informative data   no informative data  Hindgut    no informative data   120.3  Malpighian Tubules    239.1   36.1  Fat Body    no informative data   9.3  Salivary Gland    no informative data   NA  Heart    45.55   no informative data  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    9.4   NA  Testis    23.4   NA  Male Accessory Gland    no informative data   189.725  Carcass    65.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering    29 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (29) central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering  (29) carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering    29 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering    29 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering    29 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  29 central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering  29 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering    29 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering    29 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    26 embryo 04-06hr    64 embryo 06-08hr    61 embryo 08-10hr    58 embryo 10-12hr    39 embryo 12-14hr    47 embryo 14-16hr    27 embryo 16-18hr    26 embryo 18-20hr    26 embryo 20-22hr    22 embryo 22-24hr    26 larva L1    15 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    21 white prepupae new    26 white prepupae 12hr    23 white prepupae 24hr    31 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr  26 embryo 04-06hr  (64) embryo 06-08hr  (61) embryo 08-10hr  (58) embryo 10-12hr  (39) embryo 12-14hr  (47) embryo 14-16hr  (27) embryo 16-18hr  26 embryo 18-20hr  26 embryo 20-22hr    22 embryo 22-24hr  26 larva L1    15 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    21 white prepupae new  26 white prepupae 12hr    23 white prepupae 24hr  (31) pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    26 embryo 04-06hr    64 embryo 06-08hr    61 embryo 08-10hr    58 embryo 10-12hr    39 embryo 12-14hr    47 embryo 14-16hr    27 embryo 16-18hr    26 embryo 18-20hr    26 embryo 20-22hr    22 embryo 22-24hr    26 larva L1    15 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    21 white prepupae new    26 white prepupae 12hr    23 white prepupae 24hr    31 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    26 embryo 04-06hr    64 embryo 06-08hr    61 embryo 08-10hr    58 embryo 10-12hr    39 embryo 12-14hr    47 embryo 14-16hr    27 embryo 16-18hr    26 embryo 18-20hr    26 embryo 20-22hr    22 embryo 22-24hr    26 larva L1    15 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    21 white prepupae new    26 white prepupae 12hr    23 white prepupae 24hr    31 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    26 embryo 04-06hr    64 embryo 06-08hr    61 embryo 08-10hr    58 embryo 10-12hr    39 embryo 12-14hr    47 embryo 14-16hr    27 embryo 16-18hr    26 embryo 18-20hr    26 embryo 20-22hr    22 embryo 22-24hr    26 larva L1    15 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    21 white prepupae new    26 white prepupae 12hr    23 white prepupae 24hr    31 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr  26 embryo 04-06hr  (64) embryo 06-08hr  (61) embryo 08-10hr  (58) embryo 10-12hr  (39) embryo 12-14hr  (47) embryo 14-16hr  27 embryo 16-18hr  26 embryo 18-20hr  26 embryo 20-22hr    22 embryo 22-24hr  26 larva L1    15 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    21 white prepupae new  26 white prepupae 12hr    23 white prepupae 24hr  31 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    26 embryo 04-06hr    64 embryo 06-08hr    61 embryo 08-10hr    58 embryo 10-12hr    39 embryo 12-14hr    47 embryo 14-16hr    27 embryo 16-18hr    26 embryo 18-20hr    26 embryo 20-22hr    22 embryo 22-24hr    26 larva L1    15 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    21 white prepupae new    26 white prepupae 12hr    23 white prepupae 24hr    31 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    26 embryo 04-06hr    64 embryo 06-08hr    61 embryo 08-10hr    58 embryo 10-12hr    39 embryo 12-14hr    47 embryo 14-16hr    27 embryo 16-18hr    26 embryo 18-20hr    26 embryo 20-22hr    22 embryo 22-24hr    26 larva L1    15 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    21 white prepupae new    26 white prepupae 12hr    23 white prepupae 24hr    31 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    5 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    54 Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    2 embryonic S1    21 embryonic S3    22 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    10 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    45 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    27 tumorous blood cells mbn2    7 ovary fGS/OSS    24 ovary OSC    1 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (54) Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    2 embryonic S1    21 embryonic S3    22 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    10 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3  (45) eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2  (27) tumorous blood cells mbn2    7 ovary fGS/OSS    24 ovary OSC    1 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    54 Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    2 embryonic S1    21 embryonic S3    22 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    10 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    45 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    27 tumorous blood cells mbn2    7 ovary fGS/OSS    24 ovary OSC    1 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    54 Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    2 embryonic S1    21 embryonic S3    22 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    10 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    45 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    27 tumorous blood cells mbn2    7 ovary fGS/OSS    24 ovary OSC    1 ovary OSS    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    54 Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    2 embryonic S1    21 embryonic S3    22 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    10 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    45 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    27 tumorous blood cells mbn2    7 ovary fGS/OSS    24 ovary OSC    1 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (54) Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    2 embryonic S1    21 embryonic S3    22 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    10 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3  (45) eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2  27 tumorous blood cells mbn2    7 ovary fGS/OSS    24 ovary OSC    1 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    54 Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    2 embryonic S1    21 embryonic S3    22 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    10 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    45 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    27 tumorous blood cells mbn2    7 ovary fGS/OSS    24 ovary OSC    1 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    54 Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    2 embryonic S1    21 embryonic S3    22 leg disc CME L1    3 wing disc CME-W2    0 wing disc ML-DmD8    1 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    10 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    45 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    27 tumorous blood cells mbn2    7 ovary fGS/OSS    24 ovary OSC    1 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    2 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_11 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • RE67261 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003866 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1374-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for tsh Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal tsh1 tsh2757R8 lethal (with Df(2L)305) tsh12-5 lethal | embryonic stage, with Scer\GAL448Y tshScer\UAS.cGa lethal | embryonic stage | recessive tsh17 tsh82 tsh134 lethal | heat sensitive tshhs.PdZ lethal | pupal stage, with Scer\GAL4dpp.blk1 tshScer\UAS.cGa lethal | recessive tsh4 tsh8 tsh9 tsh04319 tshae-23.2 tshae-23.7 tshGal4-1 viable tsh55 tsh75 tsh179 tsh253 tshA(2)-8 tshae-l viable, with Scer\GAL4pnr-MD237 tshGD1552 Other Phenotypes Allele cell shape defective | somatic clone, with Scer\GAL4αTub84B.PZ tshScer\UAS.cGa developmental rate defective, with Scer\GAL4dpp.blk1 tshScer\UAS.cGa flightless tshae-l flightless | recessive tshae-l large body, with Scer\GAL4dpp.blk1 tshScer\UAS.cGa mitotic cell cycle defective | recessive tshA3-2-66 tshNG1 planar polarity defective (with Df(2L)305) tsh12-5 visible tsh8 tsh51 visible, with Scer\GAL4ap-md544 tshScer\UAS.cGCa visible, with Scer\GAL4bi-omb-Gal4 tshScer\UAS.cGa visible, with Scer\GAL4Bx-MS1096 tshScer\UAS.cGCa visible, with Scer\GAL4cb26 tshScer\UAS.cGa visible, with Scer\GAL4cb41 tshScer\UAS.cGa visible, with Scer\GAL4dpp.blk1 tshScer\UAS.cGa visible, with Scer\GAL4dpp.PS tshScer\UAS.cGa visible, with Scer\GAL4ey.PH tshScer\UAS.cGa visible, with Scer\GAL4GMR.PF tshScer\UAS.cGa visible, with Scer\GAL4Ser.PGF tshScer\UAS.cGa visible | dominant tsh4 tsh8 tsh9 tshA3-2-66 tshDMR.PP tshFe visible | homeotic tsh8 tsh2757R8 visible | homeotic, with Scer\GAL469B tshScer\UAS.cGa visible | recessive tsh1 tsh75 tshA3-2-66 tshae-1 tshae-l tshi71 visible | somatic clone tsh8 visible | somatic clone, with Scer\GAL4Act5C.PI tshScer\UAS.cGa visible | somatic clone, with Scer\GAL4Ubx.PdC tshScer\UAS.cGCa Phenotype manifest in Allele abdomen | segment polarity expression pattern tshcFa adult head tshDMR.PP tshFe antenna & eye | ectopic tshDMR.PP tshFe antenna | somatic clone, with Scer\GAL4Act5C.PI tshScer\UAS.cGa antennal disc, with Scer\GAL4dpp.PS tshScer\UAS.cDa tshΔZn1.Scer\UAS tshΔZn2.Scer\UAS tshΔZn3.Scer\UAS tshΔPLDLS.Scer\UAS antennal disc | ectopic | third instar larval stage, with Scer\GAL4cb26 tshScer\UAS.cGa antennal disc | ectopic | third instar larval stage, with Scer\GAL4cb41 tshScer\UAS.cGa antennal disc | ectopic | third instar larval stage, with Scer\GAL4dpp.PS tshScer\UAS.cGa antennal disc | ectopic | third instar larval stage, with Scer\GAL4Ser.PGF tshScer\UAS.cGa antennal disc | somatic clone, with Scer\GAL4Scer\FRT.Act5C tshΔZn3.Scer\UAS antennal disc | somatic clone | ventral, with Scer\GAL4Scer\FRT.Act5C tshScer\UAS.cDa tshΔZn1.Scer\UAS anterior scutellar bristle tsh1 arista tshDMR.PP tshFe coxa tshmd621 coxa (with Df(2L)305) tsh12-5 tsh3 coxa (with tshHD1) tshmd621 coxa (with tshmd621) tshHD1 cuprophilic cell, with Scer\GAL448Y tshScer\UAS.cGa denticle, with Scer\GAL469B tshScer\UAS.cGa denticle belt tsh8 tsha.cFa tshunspecified denticle belt, with Scer\GAL469B tshScer\UAS.cGa denticle belt & embryonic prothoracic segment tshunspecified denticle belt | heat sensitive tshhs.PdZ denticle belt | larval stage tsh8 denticle row 1 tsh8 denticle row 2 tsh2757R8 tsh8 denticle row 3 tsh8 denticle row 4 tsh2757R8 tsh8 denticle row 5 | supernumerary tsh2757R8 dorsal closure embryo, with Scer\GAL469B tshScer\UAS.cGa embryonic/first instar larval cuticle tsh8 embryonic/first instar larval cuticle, with Scer\GAL469B tshScer\UAS.cGa embryonic/first instar larval cuticle, with Scer\GAL4hs.PB tshScer\UAS.cGa embryonic/larval cuticle tsh8 embryonic antennal segment, with Scer\GAL469B tshScer\UAS.cGa embryonic head, with Scer\GAL469B tshScer\UAS.cGa embryonic labial segment, with Scer\GAL469B tshScer\UAS.cGa embryonic labial segment | ectopic tsh8 embryonic maxillary segment, with Scer\GAL469B tshScer\UAS.cGa embryonic prothoracic segment tsh8 embryonic prothoracic segment | ectopic, with Scer\GAL469B tshScer\UAS.cGa eye, with Scer\GAL4arm.PS, Scer\GAL4ey.PH tshdsRNA.Scer\UAS eye, with Scer\GAL4dpp.blk1 tshScer\UAS.cGa eye, with Scer\GAL4ey.PH tshScer\UAS.cGa eye | dorsal, with Scer\GAL4bi-omb-Gal4 tshScer\UAS.cGa eye | dorsal | somatic clone tsh8 eye | dorsal | somatic clone, with Scer\GAL4Act5C.PI tshScer\UAS.cGa eye | ectopic tshDMR.PP tshFe eye | ectopic, with Scer\GAL4Bx-MS1096 tshScer\UAS.cGa eye | ectopic, with Scer\GAL4cb26 tshScer\UAS.cGa eye | ectopic, with Scer\GAL4cb41 tshScer\UAS.cGa eye | ectopic, with Scer\GAL4dpp.blk1 tshScer\UAS.cGa eye | ectopic, with Scer\GAL4dpp.PS tshScer\UAS.cDa tshΔZn1.Scer\UAS tshΔZn2.Scer\UAS tshΔZn3.Scer\UAS tshΔPLDLS.Scer\UAS tshScer\UAS.cGa eye | ectopic, with Scer\GAL4MD705 tshScer\UAS.cGa eye | ectopic, with Scer\GAL4Ser.PGF tshScer\UAS.cGa eye | ectopic | somatic clone, with Scer\GAL4Act5C.PI tshScer\UAS.cGa eye | somatic clone, with Scer\GAL4Act5C.PI tshdsRNA.Scer\UAS eye | somatic clone | ventral tsh8 eye | somatic clone | ventral, with Scer\GAL4Act5C.PI tshScer\UAS.cGa eye | ventral, with Scer\GAL4bi-omb-Gal4 tshScer\UAS.cGa eye-antennal disc, with Scer\GAL4dpp.blk1 tshScer\UAS.cGa eye disc, with Scer\GAL4bi-omb-Gal4 tshScer\UAS.cGa eye disc, with Scer\GAL4ey.PH tshScer\UAS.cGa eye disc | somatic clone, with Scer\GAL4αTub84B.PZ tshScer\UAS.cGa eye disc | somatic clone | ventral, with Scer\GAL4Act5C.PI tshScer\UAS.cGa eye photoreceptor cell, with Scer\GAL4GMR.PF tshScer\UAS.cGa femur tshmd621 femur (with tshHD1) tshmd621 femur (with tshmd621) tshHD1 femur | proximal (with Df(2L)305) tsh12-5 tsh3 femur | somatic clone, with Scer\GAL4Act5C.PP tshScer\UAS.cGa femur | somatic clone, with Scer\GAL4arm.T:Hsim\VP16 tshScer\UAS.cGa haltere tsh51 tshA3-2-66 tshae-1 tshae-l haltere sclerite tshae-1 haltere sclerite (with tsh8.1) tshae-1 haltere sclerite (with tshae-1) tsh8.1 head & eye | ectopic tshDMR.PP tshFe interommatidial bristle | ectopic, with Scer\GAL4Bx-MS1096 tshScer\UAS.cGa Keilin's organ tsh1 tsh8 tsha.cFa labial segment | heat sensitive tshhs.PdZ larval abdomen tsh8 tshR6 larval abdominal segment 1 tshR6 larval antennal segment & denticle | ectopic, with Scer\GAL469B tshScer\UAS.cGa larval head, with Scer\GAL469B tshScer\UAS.cGa larval labial segment, with Scer\GAL469B tshScer\UAS.cGa larval maxillary segment & denticle | ectopic, with Scer\GAL469B tshScer\UAS.cGa larval mesothoracic segment tshR6 larval thorax tsh8 leg tshi71 tshmd621 leg (with tshHD1) tshmd621 leg (with tshmd621) tshHD1 leg & cuticle | dorsal | somatic clone, with Scer\GAL4Act5C.PP tshScer\UAS.cGa leg & cuticle | dorsal | somatic clone, with Scer\GAL4arm.T:Hsim\VP16 tshScer\UAS.cGa leg & cuticle | somatic clone, with Scer\GAL4Act5C.PP tshScer\UAS.cGa leg & cuticle | somatic clone, with Scer\GAL4arm.T:Hsim\VP16 tshScer\UAS.cGa leg & cuticle | ventral | somatic clone, with Scer\GAL4Act5C.PP tshScer\UAS.cGa leg & cuticle | ventral | somatic clone, with Scer\GAL4arm.T:Hsim\VP16 tshScer\UAS.cGa leg | ectopic tshDMR.PP tshFe maxillary palpus tsh4 tsh75 tsh8 tsh9 maxillary segment | heat sensitive tshhs.PdZ mesothoracic coxal bristle on hairy island tshmd621 mesothoracic coxal bristle on hairy island (with tshHD1) tshmd621 mesothoracic coxal bristle on hairy island (with tshmd621) tshHD1 mesothoracic coxal sensillum trichodeum tshmd621 mesothoracic coxal sensillum trichodeum (with tshHD1) tshmd621 mesothoracic coxal sensillum trichodeum (with tshmd621) tshHD1 mesothoracic femoral bract | somatic clone, with Scer\GAL4Act5C.PP tshScer\UAS.cGa mesothoracic femoral bract | somatic clone, with Scer\GAL4arm.T:Hsim\VP16 tshScer\UAS.cGa metaphase & condensed nuclear chromosome tshNG1 metathoracic coxal bristle on hairy island tshmd621 metathoracic coxal bristle on hairy island (with tshHD1) tshmd621 metathoracic coxal bristle on hairy island (with tshmd621) tshHD1 metathoracic coxal sensillum trichodeum tshmd621 metathoracic coxal sensillum trichodeum (with tshHD1) tshmd621 metathoracic coxal sensillum trichodeum (with tshmd621) tshHD1 metathoracic femoral bract | somatic clone, with Scer\GAL4Act5C.PP tshScer\UAS.cGa metathoracic femoral bract | somatic clone, with Scer\GAL4arm.T:Hsim\VP16 tshScer\UAS.cGa midgut constriction 1 tsh8 midgut constriction 2 tsh8 mitotic anaphase tshNG1 mitotic cell cycle tshA3-2-66 tshNG1 mitotic metaphase tshNG1 mouthpart tsha.cFa tshcFa photoreceptor cell | ectopic, with Scer\GAL4Bx-MS1096 tshScer\UAS.cGa photoreceptor cell | ectopic, with Scer\GAL4hs.PB tshScer\UAS.cGa photoreceptor cell | ectopic, with Scer\GAL4MD705 tshScer\UAS.cGa photoreceptor cell | somatic clone, with Scer\GAL4Act5C.PI tshScer\UAS.cGa procephalic segment | heat sensitive tshhs.PdZ prothoracic coxal bristle on hairy island tshmd621 prothoracic coxal bristle on hairy island (with tshHD1) tshmd621 prothoracic coxal bristle on hairy island (with tshmd621) tshHD1 prothoracic coxal sensillum trichodeum tshmd621 prothoracic coxal sensillum trichodeum (with tshHD1) tshmd621 prothoracic coxal sensillum trichodeum (with tshmd621) tshHD1 prothoracic femoral bract | somatic clone, with Scer\GAL4Act5C.PP tshScer\UAS.cGa prothoracic femoral bract | somatic clone, with Scer\GAL4arm.T:Hsim\VP16 tshScer\UAS.cGa prothoracic segment tsh2757R8 tsh8 T1 beard, with Scer\GAL469B tshScer\UAS.cGa T1 beard | ectopic, with Scer\GAL469B tshScer\UAS.cGa tibia | somatic clone, with Scer\GAL4Act5C.PP tshScer\UAS.cGa tibia | somatic clone, with Scer\GAL4arm.T:Hsim\VP16 tshScer\UAS.cGa trochanter tshmd621 trochanter (with Df(2L)305) tsh12-5 tsh3 trochanter (with tshHD1) tshmd621 trochanter (with tshmd621) tshHD1 ventral thoracic disc | somatic clone, with Scer\GAL4Act5C.PP tshScer\UAS.cGa ventral thoracic disc | somatic clone, with Scer\GAL4arm.T:Hsim\VP16 tshScer\UAS.cGa vertical cleft (with tsh8.1) tshae-1 vertical cleft (with tshae-1) tsh8.1 wing tsh51 tshA3-2-66 tshae-1 tshae-l wing | dorsal compartment, with Scer\GAL4ap-md544 tshScer\UAS.cGCa wing | dorsal compartment, with Scer\GAL4Bx-MS1096 tshScer\UAS.cGCa wing | somatic clone, with Scer\GAL4Ubx.PdC tshScer\UAS.cGCa wing disc, with Scer\GAL4bi-omb-Gal4 tshScer\UAS.cGa wing disc, with Scer\GAL4dpp.blk1 tshScer\UAS.cGa wing disc, with Scer\GAL4sd-SG29.1 tshScer\UAS.cWa wing hinge tshae-1 wing hinge (with tsh8.1) tshae-1 wing hinge (with tshae-1) tsh8.1 wing hinge | ectopic, with Scer\GAL4ap-md544 tshScer\UAS.cGCa wing hinge | ectopic, with Scer\GAL4Bx-MS1096 tshScer\UAS.cGCa wing hinge | ectopic, with Scer\GAL4Ubx.PdC tshScer\UAS.cGCa wing margin tshA3-2-66 wing sclerite tshae-1 wing sclerite (with tsh8.1) tshae-1 wing sclerite (with tshae-1) tsh8.1 wing sclerite | ectopic, with Scer\GAL4ap-md544 tshScer\UAS.cGCa wing sclerite | ectopic, with Scer\GAL4Bx-MS1096 tshScer\UAS.cGCa wing sclerite | ectopic, with Scer\GAL4Ubx.PdC tshScer\UAS.cGCa wing vein | ectopic tshA3-2-66
Classical Alleles ( 38 )
For All Classical Alleles Show
Allele of tsh Class Mutagen Stocks Known lesion tsh4-3 P-element activity 2 Yes tshmd621 P-element activity 2 -- tsh04319 P-element activity 1 Yes tsh3 P-element activity 1 -- tshA3-2-66 P-element activity 1 Yes tshi71 Js 1 Yes tshNP0514 1 -- tshPyR248 P-element activity 1 Yes tsh8 loss of function allele, amorphic allele - genetic evidence Delta2-3 0 Yes tshae-l loss of function allele, amorphic allele - genetic evidence spontaneous 0 Yes tsh12-5 ethyl methanesulfonate 0 -- tsh13-16 ethyl methanesulfonate 0 -- tsh134 P-element activity 0 Yes tsh179 P-element activity 0 -- tsh17 P-element activity 0 Yes tsh1 P-element activity 0 Yes tsh253 P-element activity 0 -- tsh2757R8 loss of function allele 0 -- tsh4 gamma ray 0 Yes tsh51 P-element activity 0 Yes tsh55 P-element activity 0 -- tsh70 ethyl methanesulfonate 0 -- tsh75 P-element activity 0 -- tsh8.1 P-element activity 0 Yes tsh82 P-element activity 0 Yes tsh9 ethyl methanesulfonate 0 -- tshA(2)-8 P-element activity 0 Yes tshae-1 spontaneous transposable element activity 0 Yes tshae-23.2 Delta2-3 0 Yes tshae-23.7 Delta2-3 0 Yes tshB4-2-12 P-element activity 0 -- tshc724 P-element activity 0 Yes tshFe gain of function allele P-element activity 0 Yes tshGal4-1 P-element activity 0 -- tshHD1 P-element activity 0 Yes tshNG1 N-methyl-N'-nitro-N-nitrosoguanidine 0 Yes tshR6 P-element activity Delta2-3 0 Yes tshunspecified 0 --
Alleles Carried on Transgenic Constructs ( 23 )
For All Alleles Carried on Transgenic Constructs Show
Allele of tsh Class Mutagen Stocks Known lesion tshGD1552 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tshHMS01443 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tshJF02856 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tsh+t28.4 in vitro construct - genomic fragment 0 Yes tsh+t86.4 in vitro construct - genomic fragment 0 Yes tsh220.mCa in vitro construct - regulatory fusion 0 Yes tsha.cFa in vitro construct - minigene 0 Yes tshbcd.mCa in vitro construct - site directed mutagenesis 0 Yes tshcFa in vitro construct - minigene 0 Yes tshDMR.PP in vitro construct - regulatory fusion 0 Yes tshdsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes tshhs.PdZ in vitro construct - regulatory fusion 0 Yes tshmCa in vitro construct - minigene 0 Yes tshNIG.1374R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes tshScer\UAS.cDa in vitro construct - regulatory fusion 0 Yes tshScer\UAS.cGa in vitro construct - regulatory fusion 0 Yes tshScer\UAS.cGCa in vitro construct - regulatory fusion 0 Yes tshScer\UAS.cWa in vitro construct - regulatory fusion 0 Yes tshScer\UAS.TVN.T:Avic\GFP-VN in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes tshΔPLDLS.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes tshΔZn1.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes tshΔZn2.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes tshΔZn3.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)305 (Soanes and Bell, 1999, Erkner et al., 1999, Kerridge, 1996.11.6) Df(2L)BSC150 (Christensen and Cook, 2006.3.8) Df(2L)BSC151 (Christensen and Cook, 2006.3.8) Df(2L)Exel6049 (Ryder, 2004.4.26) Df(2L)Exel7082 (Ryder, 2004.4.26) Df(2L)tsh7 (Fasano et al., 1991) Df(2L)TW161 (Soanes and Bell, 1999, Spradling et al., 1999, Fasano et al., 1991, BDGP Project Members, 1994-1999, Bilder and Scott, 1995, Kerridge, 1996.1.25, Duffy et al., 1996) In(2L)tsh3 (Kerridge, 1997.11.26)
Duplicated in	Dp(2;Y)H2 (Soanes and Bell, 1999)
Not disrupted in	Df(2L)C' (BDGP Project Members, 1994-1999) Df(2L)ltx217 (McCormick et al., 1995) Df(2L)TE38A-2 (Kerridge, 1996.11.6) Df(2L)TW65 (Soanes and Bell, 1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data reporter construct P{+Δ1-2} No P{EΔ1-2} No P{tsh-lacZ.0.8} No P{tsh-lacZ.0.22} No P{tsh-lacZ.2.0} No P{tsh-lacZ.8.0} No P{tsh-lacZ.209} No P{tsh-lacZ.481} No P{tsh-lacZ.DraI} No P{tsh-lacZ.E4.8} No P{tsh-lacZ.EB2.9} No P{tsh-lacZ.enh} No P{tsh-lacZ.HB1.0} No P{Δ1-4} No P{Δ1-5} No P{Δ2-5} No P{Δ3-5} No UAS construct P{GD1552} NA P{NIG.1374R} NA P{TRiP.HMS01443} NA P{TRiP.JF02856} NA P{UAS-tsh.dsRNA} NA P{UAS-tsh.G} NA P{UAS-tsh.GC} NA P{UAS-tsh.W} NA P{UAS-tsh-VN} NA PBac{UAS-tsh.D} NA PBac{UAS-tsh.ΔPLDLS} NA PBac{UAS-tsh.ΔZn1} NA PBac{UAS-tsh.ΔZn2} NA PBac{UAS-tsh.ΔZn3} NA heat-shock construct P{HS-tsh} NA characterization construct P{DMR-tsh.P} NA P{tsh220.mCa} NA P{tshbcd.mCa} NA P{tshmCa} NA PBac{UAS-Tio::Tsh.D} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{A92}tsh1 No P{A92}tshi71 No P{lacW}J834 No P{lacW}l(2)SH1702SH1702 No P{lacW}tsh55 No P{lacW}tsh75 No P{lacW}tsh179 No P{lacW}tsh253 No P{lacW}tshA3-2-66 No P{lacW}tshA(2)-8 No P{lacW}tshB4-2-12 No P{PyR}tshPyR248 No P{PZ}tsh8.1 No P{PZ}tsh04319 No P{PZ}tshae-23.2 No P{PZ}tshae-23.7 No insertion of enhancer trap binary system P{GawB}A4 No P{GawB}tshc724 No P{GawB}tshGal4-1 No P{GawB}tshmd621 No P{GawB}tshNP0514 No insertion of mobile activating element P{GSV1}GSd480GSd480 NA P{XP}tshd10944 NA miscellaneous insertions I{}tshae-1 NA P{DMR}tshFe NA P{SUPor-P}C380 NA P{wAR}tsh4-3 NA P{white-un2}tsh51 NA
Gene Ontology: Function, Process & Cellular Component ( 23 unique terms )
Terms Based on Experimental Evidence ( 17 terms )
Molecular Function
CV term Evidence References
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription inferred from direct assay (Alexandre et al., 1996) inferred from mutant phenotype (Alexandre et al., 1996)
Biological Process
CV term Evidence References
compound eye development inferred from mutant phenotype (Bessa and Casares, 2005) dorsal/ventral pattern formation, imaginal disc inferred from mutant phenotype (assigned by UniProtKB) (Singh et al., 2004) epidermis morphogenesis inferred from mutant phenotype (Laugier et al., 2005) eye-antennal disc development inferred from mutant phenotype (assigned by UniProtKB) (Singh et al., 2004) head involution inferred from mutant phenotype (Laugier et al., 2005) imaginal disc-derived wing morphogenesis inferred from mutant phenotype (Dworkin and Gibson, 2006) leg disc proximal/distal pattern formation inferred from mutant phenotype (assigned by UniProtKB) (Erkner et al., 1999, Wu and Cohen, 2000) Malpighian tubule stellate cell differentiation inferred from expression pattern (Liu et al., 1999, Laugier et al., 2005) midgut development inferred from mutant phenotype (assigned by UniProtKB) (Mathies et al., 1994) negative regulation of transcription from RNA polymerase II promoter inferred from mutant phenotype (Bessa et al., 2009, Alexandre et al., 1996) inferred from mutant phenotype (assigned by UniProtKB) (Soanes et al., 2001) regulation of transcription from RNA polymerase II promoter inferred from mutant phenotype (Roder et al., 1992) specification of segmental identity, head inferred from mutant phenotype (Laugier et al., 2005) specification of segmental identity, thorax inferred from mutant phenotype (Roder et al., 1992) specification of segmental identity, trunk inferred from mutant phenotype (Roder et al., 1992, Laugier et al., 2005) inferred from mutant phenotype (assigned by UniProtKB) (de Zulueta et al., 1994) wing disc proximal/distal pattern formation inferred from mutant phenotype (assigned by UniProtKB) (Soanes et al., 2001)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Bessa et al., 2009, Alexandre et al., 1996)
Terms Based on Predictions or Assertions ( 10 terms )
Molecular Function
CV term Evidence References
sequence-specific DNA binding transcription factor activity non-traceable author statement (FlyBase, 1992-) zinc ion binding inferred from electronic annotation with InterPro:IPR007087, InterPro:IPR015880 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
imaginal disc-derived leg morphogenesis traceable author statement (Milan and Cohen, 2000) negative regulation of salivary gland boundary specification traceable author statement (Bradley et al., 2001, Abrams et al., 2003) negative regulation of transcription from RNA polymerase II promoter non-traceable author statement (Swiss-Prot Project Members, 1991.8.1) regulation of transcription from RNA polymerase II promoter non-traceable author statement (FlyBase, 1992-) salivary gland development non-traceable author statement (Nelson, 2003) specification of segmental identity, abdomen non-traceable author statement (Swiss-Prot Project Members, 1991.8.1) specification of segmental identity, thorax non-traceable author statement (Swiss-Prot Project Members, 1991.8.1)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (FlyBase, 1992-, Swiss-Prot Project Members, 1991.8.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
tsh - CG11266 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) tsh - CG3817 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) tsh - CG7878 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) tsh - Imp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) tsh - Rpb8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) tsh - Sec23 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) tsh - Trap1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) tsh - exd pull down, autoradiography (Bessa et al., 2002) tsh - hth pull down, autoradiography, anti bait coimmunoprecipitation, western blot (Bessa et al., 2002) tsh - mRpS24 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) tsh - mRpS33 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) tsh - mod anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions tsh allele Gene References tsh8 Antp (Bhojwani et al., 1997) brk (Saller et al., 2002) ci (Angelats et al., 2002) Dfd (Robertson et al., 2004) tsh17 Antp (Bhojwani et al., 1997) tsh75 Antp (Bhojwani et al., 1997) tshScer\UAS.cGa ara (Singh et al., 2004) arm (Erkner et al., 1999) ci (Angelats et al., 2002) Dl (Singh et al., 2004) hth (Singh et al., 2002) Ser (Singh et al., 2004) spen (Wiellette et al., 1999) wg (Singh et al., 2002) tshunspecified spen (Wiellette et al., 1999) unspecified arm (Angelats et al., 2002) eya (Pan and Rubin, 1998) mod (Alexandre et al., 1996) so (Pan and Rubin, 1998) tio (Laugier et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61373 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0003866 DroID - A comprehensive database of gene and protein interactions. • FBgn0003866 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35430 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P22265
Orthologs
OrthoDB Orthologs (38) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6Z8ZFZ Dipteran inclusive ortholog search EOG6JQ45Q Insect inclusive ortholog search EOG6W3RH0 Arthropod inclusive ortholog search EOG66M90N Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6Z8ZFZ)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\CG34383     Drosophila melanogaster fruit fly  Dmel\tsh     Drosophila simulans   Dsim\GD15154   Y Drosophila simulans   Dsim\GD18927   Y Drosophila sechellia   Dsec\GM24128   Y Drosophila erecta   Dere\GG19805   Y Drosophila yakuba   Dyak\GE26294   Y Drosophila ananassae   Dana\GF16879   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA15277   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA30134   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA22354   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA18005   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA22338   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA28833   Y Drosophila persimilis   Dper\GL23087   Y Drosophila willistoni   Dwil\GK14196   Y Drosophila virilis   Dvir\GJ14476   Y Drosophila mojavensis   Dmoj\GI23162   Y Drosophila grimshawi   Dgri\GH14270   Y
Orthologs in non-Drosophila Dipterans (EOG6JQ45Q)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL003252 Y Anopheles gambiae Malaria mosquito  Agam\AGAP005285 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ801513 Y
Orthologs in non-Dipteran Insects (EOG6W3RH0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB53562 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012509 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH22599 Y Atta cephalotes Leafcutter ant  Acep\ACEP24207 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO15065 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL25867 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14414 Y Linepithema humile Argentine ant  Lhum\LH19970 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB14314 Y Solenopsis invicta Red fire ant  Sinv\SINV16596 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI001170 Y Bombyx mori Silkmoth  Bmor\BGIBMGA011806 Y Bombyx mori Silkmoth  Bmor\BGIBMGA000203 Y Pediculus humanus Human body louse  Phum\PHUM262070 Y Tribolium castaneum Red flour beetle  Tcas\TC010799 Y
Orthologs in non-Insect Arthropods (EOG66M90N)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW001499 Y
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD24333 Drosophila sechellia Dsec\GM16144 Drosophila erecta Dere\GG21347 Drosophila yakuba Dyak\GE12928 Drosophila ananassae Dana\GF21464 Drosophila pseudoobscura pseudoobscura Dpse\GA12497 Drosophila persimilis Dper\GL25653 Drosophila willistoni Dwil\GK18714 Drosophila virilis Dvir\GJ11344 Drosophila mojavensis Dmoj\GI17699 Drosophila grimshawi Dgri\GH22172
Stocks & Reagents
Stocks Listed in FlyBase ( 13 )
Bloomington	28022 y1 v1; P{TRiP.JF02856}attP2 8883 In(2L)tsh3, l(2)34Dbtsh3 tsh3/CyO 10842 y1 w*; P{lacW}tshA3-2-66/CyO 11370 P{PZ}tsh04319 cn1/CyO; ry506 35030 y1 sc* v1; P{TRiP.HMS01443}attP2 3040 y1 w1118; P{GawB}tshmd621/CyO; P{UAS-y.C}MC1/TM2 1296 C(1)M3, y1/Dp(1;Y)y+; P{wAR}tsh4-3; awdK
Kyoto	103629 w*; P{GawB}tshNP0514 / CyO 109143 y1 w67c23; P{PyR}tshPyR248 / CyO 106797 y1 w1118; P{GawB}tshmd621/CyO; P{UAS-y.C}MC1/TM2 106268 C(1)M3, y1/Dp(1;Y)y+; P{wAR}tsh4-3; awdK
VDRC	v16892 w1118; P{GD1552}v16892
	More stocks available...
Genomic Clones ( 2 )
	BACR03L08 BACR09H20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 52 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE67261
Other clones	DPiM_FI01023 FI01023
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD44646 LP07848 LP11282 RE71662 RH28413 RH57207 RH59387 RH69068 SD15455
Other clones	31635 47188 61920 62155 65346 65559 103188 125496 146232 149849 159491 214255.13 CK01.4A.B8 CK01.24B.D1 CK01.24B.G1 CK01.32C.H7 CK01.119A.B8 CK01.170B.D2 CK00422 CK01473 EK029109 EK043935 EK043944 EK104445 EK140840 EK141624 EK172909 EK175121 EK185529 EK187744 EK192042 EK192415 EK249143 EK255854 EK301008 EP07425 EP18462 FGC020A02 FGC027E11 FGH101F09
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003866 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35430
Antibody Information
Other Information
Discoverer
	Mohr, 24th Nov. 1926.
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: tsh CG1374 (Deng, 1999.11)
Source for database merge of	Source for merge of: tsh ae (Soanes and Bell, 2000)
Additional comments	Complementation tests between tshae-l and various other tsh alleles define a distinct complementation group though molecular analysis places a tshae-l lesion very close to the 3' end of the tsh transcription unit, between the 3' end of tsh and previously defined regulatory sequences. (Soanes and Bell, 1999)
Other Comments
	tsh is required in undifferentiated cells for eye specification, but its expression must later be turned off to allow retinal differentiation. (Bessa and Casares, 2005) dsRNA made from templates generated with primers directed against this gene and injected into embryos on the dorsal side causes a phenotype at the larval stage. These larvae have no T1 denticle beard and also show a reduced number of denticle rows in the abdominal segments compared to wild type. (Laugier et al., 2005) tsh and tio repress each other's expression. (Laugier et al., 2005) The primary signal for repression of tsh expression during wing development is wg. (Zirin and Mann, 2004) Different combinations of the proteins ci, tsh and arm appear to be employed for the specification of naked cuticle at distinct positions both along the anterior posterior axis and within individual trunk segments. (Angelats et al., 2002) ci, tsh and arm may act as a complex. (Angelats et al., 2002) tsh is required for thr formation of proximal leg segments, but has no role in boundary formation. (Wu and Cohen, 2000) tsh contributes to the differences in cell-cell adhesion between proximal and distal leg cells. (Erkner et al., 1999) wg signaling promotes the phosphorylation and the nuclear accumulation of tsh protein. (Gallet et al., 1999) spen cooperates with Antp and tsh to repress head-like sclerites in the thorax and promote thoracic identity. (Wiellette et al., 1999) tsh protein binds to, and requires, arm protein for the naked cell-fate choice in the larval trunk. (Gallet et al., 1998) tsh and ey induce the expression of each other, acting in a gene network that functions to specify eye identity. (Pan and Rubin, 1998) Targeted expression of tsh in imaginal discs is sufficient to induce ectopic eye formation in non-eye tissues. tsh functions with the other eye specification genes ey, so and dac to specify eye identity. (Pan and Rubin, 1998) tsh specifies adult head segments by repressing Antp expression. (Bhojwani et al., 1997) mod is a genetic target of tsh. A biological role of tsh is to repress mod expression in the ectoderm and this negative control is performed independently of Scr. tsh protein binds to specific DNA sequences within a 5' mod regulatory region that reproduces tsh-dependent expression in the ectoderm. (Alexandre et al., 1996) P{wAR}4-3 localizes to the 3' end of the tsh gene. (Kerridge, 1996.1.31) tsh expression in the visceral mesoderm is required for the development of anterior and central midgut structures and is regulated by homeotic genes and dpp and wg. (Mathies et al., 1995) tsh may act as a cofactor for homeotic function, acting in parallel to Antp in epidermal enhancer activity in the thorax while playing an antagonistic role to Ubx in somatic mesoderm. (McCormick et al., 1995) A homeotic response element (HOMRE) has been identified and characterized within the tsh gene. Different homeotic proteins control tsh enhancer activity in different tissues. (McCormick et al., 1995) Expression pattern of Ecol\lacZ from silencer detector insertion P{lacW}A(2)-6 reflects that of tsh. (Sun et al., 1995) Salivary gland induction by Scr in the trunk is limited by tsh. (Andrew et al., 1994) tsh acts in a partially redundant way with certain homeotic complex proteins but co-operates with them for the establishment of specific segment types. The tsh product and homeotic complex proteins regulate common sets of downstream target genes. (de Zulueta et al., 1994) tsh is necessary for proper formation of anterior and central midgut structures. Antp, Ubx, abd-A, dpp and wg are required for proper tsh expression. The control of tsh by Ubx, abd-A and probably also by Antp is mediated by secreted signaling molecules. (Mathies et al., 1994) tsh is a target gene of the homeotic selector genes and is regulated by exd. (Rauskolb and Wieschaus, 1994) Ectopic expression of dpp eliminates Scr and Antp expression, attenuating abd-A expression, inducing Ubx, dpp, wg and tsh expression in the visceral mesoderm and inducing lab expression in the apposing endoderm. The result is failure of all of the morphogenetic events except formation of midgut constriction 2. (Staehling-Hampton and Hoffmann, 1994) In the epidermis tsh is expressed in a parasegmental and then a segmental register whereas in the CNS its expression remains parasegmental. The maintenance of tsh expression in the posterior compartments of trunk segments is dependent on the common function of Antp and the bithorax complex genes. Scr and lab are expressed ectopically in embryos deficient for tsh and Antp. Antp and Ubx act independently of tsh for the determination of trunk identity, although tsh is essential for the complete function of the trunk homeotic genes. (Roder et al., 1992) tsh is required for normal development of the cells giving rise to the labial prothoracic region, ventral trunk region and anal opening. (Fasano et al., 1991) Mutants have outheld wings.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078891 RefSeq (Proteins) • NP_523615 DDBJ / EMBL / GenBank • AAA28983 AAF57236 AAS64735 AAT94430 BI484200 BT015201 G00738 M57496 Entrez Gene - A searchable database of RefSeq genes. • 35430 UniProtKB/Swiss-Prot • P22265
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, C2H2-like (IPR015880) Zinc finger, C2H2 (IPR007087) Teashirt (IPR027009) Teashirt family (IPR027008) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE67261
Linkouts
	BioGRID - A database of protein and genetic interactions • 61373 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0003866 DroID - A comprehensive database of gene and protein interactions. • FBgn0003866 DRSC - Results from RNAi screens. • FBgn0003866 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003866 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1374-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003866 FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways • 103933 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35430 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /gene/teashirt.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35430 modMine - Data generated by the modENCODE project. • FBgn0003866 REDfly - A database of transcriptional regulatory elements. • FBgn0003866 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P22265
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	ae (Soanes and Bell, 1999, Lindsley and Zimm, 1992) ael (Soanes and Bell, 1999) CG1374 (Datta et al., 2011, Perkins et al., 2009, Ni et al., 2011.7.19) l(2)04319   l(2)B4-2-12   tsh (Sanders et al., 2008, Dworkin and Gibson., 2006, Venken, 2006, Jordan et al., 2006, Kumar and Luhur, 2007, Kumar and Salzer, 2007, Kumar and Datta, 2007, Noro et al., 2006, Dworkin and Gibson, 2006, Culi et al., 2006, Roederer et al., 2005, Friedrich, 2006, Kim et al., 2006, Akimoto et al., 2005, Puetz et al., 2005, Estella and Mann, 2010, Takacs et al., 2008, Anderson and Kumar, 2008, Chauvet et al., 2000, Datta et al., 2011, Minidorff et al., 2007, Datta et al., 2011, Rubinstein et al., 2010, Zhang et al., 2011, Bao and Friedrich, 2009, Chen et al., 2008, Patel et al., 2007, Coiffier et al., 2008, Tanaka et al., 2007, Taghli-Lamallem et al., 2007, Merabet et al., 2007, Peng et al., 2007, Salzer and Kumar, 2009, Firth and Baker, 2009, Zirin and Mann, 2007, Oktaba et al., 2008, Campbell et al., 2010, Terriente et al., 2008, Dichtel-Danjoy et al., 2009, Bessa et al., 2009, Ayroles et al., 2009, Perea et al., 2009, Fang et al., 2009, Peng et al., 2009, Datta et al., 2009, Gambetta et al., 2009, Grieder et al., 2009, Lopes and Casares, 2010, Grad et al., 2004, Wang et al., 2004, Cherbas et al., 2011, Oros et al., 2010, Brockmann et al., 2011, Cunha et al., 2010, Berni et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Nfonsam et al., 2012, Hudry et al., 2012) Tsh (Lan et al., 2007, Venken et al., 2006, Hudry et al., 2011, Luque and Milan, 2007, Ayala and Bach, 2008, Datta and Kumar, 2008, Datta et al., 2011, Nusinow et al., 2008, Rafel and Milán, 2008, McKay et al., 2009, Benítez et al., 2009, Urbano et al., 2009, Gambetta et al., 2009, Campbell et al., 2009, Zirin and Mann, 2007, Papadopoulos et al., 2011) T shirt (Chinnadurai, 2003)
Name Synonym	aeroplane (Lindsley and Zimm, 1992) teashirt (Pearson et al., 2005, Jordan et al., 2006, Kumar and Luhur, 2007, Kumar and Salzer, 2007, Kumar and Datta, 2007, Noro et al., 2006, Dworkin and Gibson, 2006, Roederer et al., 2005, Friedrich, 2006, Akimoto et al., 2005, Estella and Mann, 2010, Takacs et al., 2008, Chauvet et al., 2000, Datta et al., 2011, Bao and Friedrich, 2009, Sandmann et al., 2006, Patel et al., 2007, Coiffier et al., 2008, Tanaka et al., 2007, Zirin and Mann, 2007, Oktaba et al., 2008, Dichtel-Danjoy et al., 2009, Bessa et al., 2009, Gambetta et al., 2009, Datta et al., 2009, Lopes and Casares, 2010, Wang et al., 2004, Gautam and Tapadia, 2010, Oros et al., 2010, Brockmann et al., 2011, Hudry et al., 2012) Teashirt (Hudry et al., 2011, Luque and Milan, 2007, Ayala and Bach, 2008, Datta et al., 2011, Ishihara and Shibata, 2008, Taghli-Lamallem et al., 2007, Rafel and Milán, 2008, Urbano et al., 2009, Peng et al., 2009, Zirin and Mann, 2007, Dougherty et al., 2008) T-shirt (Foronda et al., 2009)
Secondary FlyBase IDs
	FBgn0000058 FBgn0010570 FBgn0013543
References ( 293 )
Generate a list of	All references relating to this gene ( 293 )
List References by type	Research paper  ( 164 ) Supplementary material  ( 10 ) Review  ( 42 ) Personal communication to FlyBase  ( 20 ) Abstract  ( 46 ) FlyBase analysis  ( 2 ) Book  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 3 ) Protein sequence record  ( 1 ) Website  ( 1 ) Conference report  ( 1 )
Recent research papers ( 13 )
	Schaub and Frasch, 2013, Dev. Biol. 376(2): 245--259 Org-1 is required for the diversification of circular visceral muscle founder cells and normal midgut morphogenesis. [FBrf0221030] Staller et al., 2013, Genetics 193(1): 51--61 Depleting Gene Activities in Early Drosophila Embryos with the "Maternal-Gal4-shRNA" System. [FBrf0220341] Berni et al., 2012, Curr. Biol. 22(20): 1861--1870 Autonomous circuitry for substrate exploration in freely moving Drosophila larvae. [FBrf0219792] Fisher et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(52): 21330--21335 DNA regions bound at low occupancy by transcription factors do not drive patterned reporter gene expression in Drosophila. [FBrf0220344] Hudry et al., 2012, PLoS Biol. 10(6): e1001351 Hox Proteins Display a Common and Ancestral Ability to Diversify Their Interaction Mode with the PBC Class Cofactors. [FBrf0218732] Nfonsam et al., 2012, PLoS ONE 7(8): e44583 Analysis of the Transcriptomes Downstream of Eyeless and the Hedgehog, Decapentaplegic and Notch Signaling Pathways in Drosophila melanogaster. [FBrf0219414] Brockmann et al., 2011, Dev. Dyn. 240(1): 75--85 Regulation of ocellar specification and size by twin of eyeless and homothorax. [FBrf0212641] Cherbas et al., 2011, Genome Res. 21(2): 301--314 The transcriptional diversity of 25 Drosophila cell lines. [FBrf0213077] Datta et al., 2011, Dev. Biol. 360(2): 391--402 A dissection of the teashirt and tiptop genes reveals a novel mechanism for regulating transcription factor activity. [FBrf0216557] Datta et al., 2011, Evol. Dev. 13(1): 58--71 Differential selection within the Drosophila retinal determination network and evidence for functional divergence between paralog pairs. [FBrf0212714] Hudry et al., 2011, BMC Biol. 9: 5 Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay. [FBrf0213066] Papadopoulos et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(29): 11959--11964 Functional synthetic Antennapedia genes and the dual roles of YPWM motif and linker size in transcriptional activation and repression. [FBrf0214342] Zhang et al., 2011, PLoS ONE 6(7): e22278 Yki/YAP, Sd/TEAD and Hth/MEIS Control Tissue Specification in the Drosophila Eye Disc Epithelium. [FBrf0214606] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...