Gene Dmel\ttk

General Information
Symbol	Dmel\ttk	Species	D. melanogaster
Name	tramtrack	Annotation symbol	CG1856
Feature type	protein_coding_gene	FlyBase ID	FBgn0003870
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	100D1-100D1	Sequence location	3R:27,539,606..27,561,114 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene tramtrack is referred to in FlyBase by the symbol ttk (CG1856, FBgn0003870). It has the cytological map location 100D1. Its sequence location is 3R:27539606..27561114. Its molecular function is described as: specific RNA polymerase II transcription factor activity; transcription repressor activity; protein binding; chromatin binding; specific transcriptional repressor activity; zinc ion binding; nucleic acid binding. It is involved in the biological processes: peripheral nervous system development; R7 cell development; dorsal appendage formation; cell fate determination; regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; dendrite morphogenesis; neuron development; ovarian follicle cell development. 131 alleles are reported. The phenotypes of these alleles are annotated with 85 unique terms, many of which group under: peripheral nervous system; nervous system; embryonic nervous system; adult segment; organ system; adult mesothoracic segment; embryonic glial cell; imaginal precursor; larval abdominal segment; cone cell. It has 6 annotated transcripts and 6 annotated polypeptides.
External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Ttk: Tramtrack Gene encodes a zinc-finger protein binding to a number of sites involved in the transcriptional control of fushi-tarazu in the Antennapedia complex.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 100D1-100D1 Limits computationally determined from genome sequence between P{EP}pygo^EP1076 and P{PZ}ttk⁰²⁶⁶⁷&P{lacW}ttk^j6C7
Experimentally Determined Cytological Location
Cytogenetic map Notes References 100D1-100D2 (determined by in situ hybridisation) (Spradling et al., 1999) 100D-100D (determined by in situ hybridisation) (Szeged Stock Center, 1998-) 100D-100D (determined by in situ hybridisation) (Salzberg et al., 1997) 100D-100D (determined by in situ hybridisation) (Deak et al., 1997) 100D1-100D2 (determined by in situ hybridisation) (Giesen et al., 1997) 100D1-100D2 (determined by in situ hybridisation) (Lai et al., 1996) 100D-100D (determined by in situ hybridisation) (Brown, 1992) 100D1-100D2 100D2--3 (BDGP Project Members, 1994-1999) 100D-100D (determined by in situ hybridisation) (Xiong and Montell, 1993) 100D-100D (determined by in situ hybridisation) (Brown et al., 1991) 100D3-100D4 (determined by in situ hybridisation) (Harrison and Travers, 1990)
Experimentally Determined Recombination Data
Location	3-102.7 (Salzberg et al., 1994) 3- (Horn et al., 2003)
Left of (cM)
Right of (cM)	Med (Hudson et al., 1998)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: Med+ anon-H31? anon-I7114? awd? ttk? (Hudson et al., 1998) Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\ttk for information on other features
Comments on Gene Model
	EST data support existence of multiple transcripts
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ttk-RA FBtr0085829 NM_170564 3905 813 ttk-RB FBtr0085827 NM_170565 3820 813 ttk-RC FBtr0085828 NM_080172 4111 643 ttk-RD FBtr0085830 NM_170567 4196 643 ttk-RE FBtr0085825 NM_170566 3883 813 ttk-RF FBtr0085826 NM_170568 4174 643
Additional Transcript Data & Comments
Reported size (kB)	5.0, 4.2 (northern blot) (Xiong and Montell, 1993) 4.2 (longest cDNA) (Read and Manley, 1992)
Comments	cDNAs isolated from an eye disc library differ in their 5\\' and 3\\' UTRs from the cDNAs isolated earlier from an embryonic library (FBrf55940), but are predicted to encode the same polypeptides. (Xiong and Montell, 1993, Xiong and Montell, 1993)
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein ttk-PA FBpp0085190 88.4 813 6.19 NP_733443 AAF57180 ttk-PB FBpp0085188 88.4 813 6.19 NP_733444 AAF57179 ttk-PC FBpp0085189 68.8 643 7.65 NP_524911 AAF57182 ttk-PD FBpp0085191 68.8 643 7.65 NP_733446 AAF57181 ttk-PE FBpp0085186 88.4 813 6.19 NP_733445 AAN14282 ttk-PF FBpp0085187 68.8 643 7.65 NP_733447 AAN14283
Additional Polypeptide Data & Comments
Reported size (kD)	813, 643 (aa); 88, 69 (kD predicted) (Xiong and Montell, 1993) 811, 641 (aa); 97 (kD observed); 88 (kD predicted) (Read and Manley, 1992) 641 (aa); 69 (kD predicted) (Harrison and Travers, 1990) 641 (aa) (Brown et al., 1991)
Comments	The ttk⁺P881 protein has a predicted molecular weight of 88 kD, although it runs about 50% slower than predicted on a polyacrylamide-SDS gel. The consensus DNA binding sites of the ttk⁺P881 and ttk⁺P641 proteins differ. The ttk⁺P641 protein binds sites in the eve promoter and autoregulatory element, while the ttk⁺P881 protein binds a region in the en promoter. (Read and Manley, 1992) As absence of ttk⁺P811 correlates with supernumerary R7 photoreceptor cells, this protein may be required for suppression of genes which promote the R7 fate. (Xiong and Montell, 1993) ttk⁺P641 may repress expression of genes incompatible with the photoreceptor cell fate. (Xiong and Montell, 1993) Three binding sites for ttk protein were detected in the ftz zebra element. Three additional binding sites were detected in the ftz promoter region. (Harrison and Travers, 1990) Although the ttk⁺P641 protein has a predicted molecular weight of 69kD, it runs at about 97 kD on a polyacrylamide-SDS gel. The zinc finger motifs, and the consensus DNA binding sites of the ttk⁺P881 and ttk⁺P641 proteins differ. The ttk⁺P641 protein binds sites in the eve promoter and autoregulatory element, while the ttk⁺P881 protein binds a region in the en promoter. (Read and Manley, 1992)
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0003870
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • BTB/POZ fold (IPR011333) Zinc finger, C2H2-type/integrase, DNA-binding (IPR013087) Zinc finger, C2H2-like (IPR015880) BTB/POZ-like (IPR000210) Zinc finger, C2H2-type (IPR007087) BTB/POZ (IPR013069) HMG-I and HMG-Y, DNA-binding (IPR000637) PDB - Protein Data Bank. An information portal to biological macromolecular structures • 2DRP TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles • T00843 T00844
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141202 AA141203 AA390579 AA392395 AA441484 AA695379 AA696379 AA697110 AA950222 AE003779 AAF57179 AE003779 AAF57180 AE003779 AAF57181 AE003779 AAF57182 AE003779 AAN14282 AE003779 AAN14283 AF006649 AI124297 AI238209 AI258039 AI260063 AI532881 AQ025128 AQ034094 AQ073312 AW941964 AW942904 AY094762 AAM11115 AY122169 AAM52681 AY863929 AY863930 AY863931 AY863932 AY863933 AY863934 AY863935 AY863936 AY863937 AY863938 AY863939 AY863940 AY863941 AY863942 AY863943 AY863944 AY863945 AY863946 AY863947 AY863948 AY863949 AY863950 AY863951 BF486941 BI161772 BI161773 BI163305 BI164804 BI166318 BI167989 BI170311 BI171259 BI227822 BI228898 BI228901 BI229661 BI235078 BI238162 BI238371 BI238427 BI356109 BI357077 BI357087 BI361688 BI362314 BI362647 BI366138 BI371469 BI373830 BI374086 BI375436 BI481914 BI482134 BI483924 BI484659 BI485958 BI574369 BI577169 BP541496 BP546131 BT001723 AAN71478 BT023939 BT025183 CK01338 CK131440 CK661486 CO178012 CO179644 CO188089 CO188496 CO188905 CO261921 CO267218 CO271104 CO271324 CO273344 CO273503 CO273581 CO274924 CO275008 CO277438 CO278289 CO279950 CO281846 CO282530 CO283173 CO283450 CO289434 CO292799 CO294516 CO294887 CO299553 CO300392 CO303276 CO303582 CO304467 CO308777 CO309078 CO310886 CO313027 CO313885 CO314077 CO314134 CO316801 CO318433 CO318665 CO320916 CO321957 CO322853 CO325470 CO331571 CO332128 CO332234 CO333626 CO339127 CO339977 CO340237 CO340806 CZ466544 CZ466640 CZ466793 CZ467174 CZ467212 CZ472781 CZ472821 CZ473573 CZ473597 CZ475463 CZ475516 CZ475873