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General Information
Symbol
Dmel\αTub84B
Species
D. melanogaster
Name
α-Tubulin at 84B
Annotation Symbol
CG1913
Feature Type
FlyBase ID
FBgn0003884
Gene Model Status
Stock Availability
Gene Snapshot
In progress.Contributions welcome.
Also Known As
α-tubulin, tubulin, α1-tubulin, α tubulin, α-Tub
Key Links
Genomic Location
Cytogenetic map
Sequence location
3R:7,086,599..7,088,839 [+]
Recombination map
3-48
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the tubulin family. (P06603)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
Summaries
Gene Group (FlyBase)
ALPHA TUBULINS -
Tubulins are cytoskeletal proteins. α- and β-tubulin heterodimers polymerise to form microtubules, the roles of which include mechanical strength, intracellular trafficking and chromosome segregation. (Adapted from PMID:1121746).
Protein Function (UniProtKB)
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
(UniProt, P06603)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
αTub84B (α1t)
A structural gene for α-Tubulin; present in one copy per haploid genome; transcribed into mRNA in the oocyte and the embryo, reaching a maximum concentration 6-9 hr after the egg is laid (Kalfayan and Wensink, 1982). A variety of post-translational modifications of αTub84b protein found in all tissues, some of which are tissue-specific (Matthews et al., 1989). The gene appears to be constitutively expressed, its functions being common to most cells; the resulting tubulins are very similar to those of other animal species (Theurkauf et al., 1986). Mutations in αTub84B have been induced as lethals over Df(3R)Scx2. Alleles αTub84B1 and αTub84B3 produce proteins that are electrophoretic variants of the wild-type tubulin. Based on the extent of lethality and sterility found in interallelic heterozygotes and in heterozygotes over wild type, the severity of αTub84B alleles can be ordered as follows: αTub84B6 < αTub84B1 < αTub84B2 < αTub84B4 < αTub84B5 = αTub84B3 (Matthews and Kaufman, 1987). Maternal-effect as well as zygotic lethality is shown by the more severe alleles. In the less severe hypomorphic alleles, heterozygotes die late in pupal life or early in adult life, the adults showing head, thoracic, or abdominal defects, bristle abnormalities, leg tremors, and sterility (Matthews and Kaufman, 1987).
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\αTub84B or the JBrowse view of Dmel\αTub84B for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0081639
1752
450
Additional Transcript Data and Comments
Reported size (kB)
1.75 (unknown)
2.250 (sequence analysis)
2.0 (northern blot)
1.3 (unknown)
1.8 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0081153
49.9
450
4.78
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with Ote (PubMed:22751930).
(UniProt, P06603)
Post Translational Modification
Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling (By similarity).
(UniProt, P06603)
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\αTub84B using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (14 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000172598
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR003008, InterPro:IPR008280
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000172598
(assigned by GO_Central )
Biological Process (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000172598
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000172598
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000172598
(assigned by GO_Central )
Cellular Component (7 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000172598
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000172598
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference

Comment: reference states 0-12 hr AEL

Comment: reference states 0-21 hr AEL

Additional Descriptive Data
αTub84B transcript is detected in all developmental stages.
The αTub84Btranscript is expressed at all stages of development, with highest levelsdetected betweeen 0 and 6 hours of embryonic development, and high levelsbetween 9 and 18 hours of embryonic development and the first and second larvalinstars.
Expression ofαTub84B is abundant throughout development, with the highest concentrationof transcript expressed at 6-9 hours of embryonic development.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference

Comment: reference states 0-2 hr AEL

Comment: reference states 9-13 hr AEL

Comment: reference states 16-20 hr AEL

Additional Descriptive Data
localization of acetylated or glutamylated tubulin detected; found only in stabilized microtubules.
To compare synthesis and accumulation of α-tubulin proteins, 35S</up>methionine radiolabeled tissues were subjected to Western analysis using monoclonal anti-α-tubulin antibody. The predominant α-tubulin protein in all tissues and stages examined is αTub84B protein. The protein did not accumulate in the adult head.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from direct assay
Expression Deduced from Reporters
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{αTub84B-QF2.P}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{αTub84B-QF2w.P}
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{CXY21.29+4a}X15
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{CXY21.29+33}X15
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{Tub-EGFP.B}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{Tub-EGFP.ban}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{tubP-GAL4}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{tub-QS.P}
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\αTub84B in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
synapse & microtubule, with Scer\GAL4how-24B
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (9)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
10 of 15
Yes
Yes
8 of 15
No
Yes
8 of 15
No
Yes
7 of 15
No
Yes
7 of 15
No
Yes
 
7 of 15
No
Yes
4 of 15
No
No
4 of 15
No
Yes
3 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
10 of 15
Yes
Yes
8 of 15
No
Yes
7 of 15
No
Yes
7 of 15
No
Yes
6 of 15
No
Yes
4 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
Rattus norvegicus (Norway rat) (10)
10 of 13
Yes
Yes
7 of 13
No
Yes
5 of 13
No
Yes
4 of 13
No
Yes
4 of 13
No
No
3 of 13
No
Yes
3 of 13
No
Yes
3 of 13
No
Yes
3 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (15)
6 of 12
Yes
Yes
4 of 12
No
Yes
4 of 12
No
Yes
3 of 12
No
Yes
3 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
No
2 of 12
No
No
2 of 12
No
Yes
2 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (14)
7 of 15
Yes
Yes
7 of 15
Yes
Yes
7 of 15
Yes
Yes
7 of 15
Yes
Yes
5 of 15
No
Yes
5 of 15
No
Yes
5 of 15
No
Yes
4 of 15
No
No
3 of 15
No
No
3 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (11)
11 of 15
Yes
No
6 of 15
No
Yes
5 of 15
No
Yes
5 of 15
No
Yes
5 of 15
No
Yes
4 of 15
No
Yes
4 of 15
No
No
4 of 15
No
Yes
4 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (6)
8 of 9
Yes
Yes
5 of 9
No
Yes
5 of 9
No
Yes
4 of 9
No
Yes
3 of 9
No
Yes
2 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
13 of 15
Yes
Yes
9 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
10 of 12
Yes
Yes
10 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091907W2 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila simulans
Drosophila sechellia
Drosophila sechellia
Drosophila erecta
Drosophila erecta
Drosophila yakuba
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091504K1 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Anopheles gambiae
Malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04D6 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X049T )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0736 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (11)
8 of 10
8 of 10
6 of 10
4 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 4 )
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with Ote (PubMed:22751930).
    (UniProt, P06603 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-48
    Cytogenetic map
    Sequence location
    3R:7,086,599..7,088,839 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    84B2-84B2
    Limits computationally determined from genome sequence between P{lacW}l(3)L2100L2100 and P{EP}EP3060EP3060
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    84B-84B
    (determined by in situ hybridisation)
    84B4-84C2
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Notes
    Mapping based on seven recombinants.
    Stocks and Reagents
    Stocks (25)
    Genomic Clones (9)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (1350)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for identity of: αTub84B CG1913
    Source for database merge of
    Source for merge of: αTub84B BEST:LD32507
    Additional comments
    l(3)03076 may correspond to αTub84B: the P{PZ}l(3)0307603076 insertion maps within the transcription unit.
    Other Comments
    RNAi screen using dsRNA made from templates generated with primers directed against this gene in S2 cell results in dim staining of microtubules, and a short monopolar spindle. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a greater than three-fold increase in AttA activity in response to heat-killed E.coli after ecdysone treatment in S2 cells.
    RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
    In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
    Though αTub84B and αTub85E are very similar in sequence they are functionally distinct. αTub85E can provide function in the male germ line of generic microtubule arrays but lacks a subset of the functional properties possessed by specific features of microtubule assembly unique to the male germ cells.
    αTub84B staining is used to analyse microtubule configuration during the first division of the zygote to investigate the organisation of the first cleavage spindle and the origin of the functional centrosome.
    Mixtures of ncd or Khc motor domain treated with the zero-length cross linker EDC generates covalently cross linked products of ncd or Khc with βTub56D and αTub84B. These results indicate that kinesin family motors of opposite polarity interact with both βTub56D and αTub84B and support a model in which some portion of each ncd or Khc proteins motor domain overlaps adjacent βTub56D and αTub84B subunits.
    Investigation of the proteins that bind the regulatory sequences of αTub84B. DNaseI footprinting assays demonstrate Trl binds to tubulin element 2 (TE2) and the intron of αTub84B, but not to tubulin element 1 (TE1) and Tbf1 binds to tubulin element 1 (TE1) and tubulin element 2 (TE2) of αTub84B.
    Two upstream regions, tubulin element 1 (TE1) and tubulin element 2 (TE2), and an intron activate expression of αTub84B. These regions act ubiquitously to insulate from position effects and to activate transcription.
    Both meiosis and cleavage stage mitoses are severely affected by mutations that result in a substantial decrease in the αTub67C/αTub84B+αTub84D ratio, though an increase has little effect on meiosis while still disrupting mitotic spindle formation.
    Total α-tubulin levels remain high throughout embryogenesis. Constitutive isotypes are more stable than maternal isotypes in most tissues.
    Apical localization of pair-rule transcripts restricts lateral protein diffusion allowing pair-rule proteins to define sharp boundaries and precise spatial domains.
    An allele of αTub84B has been identified due to second site non-complementation of alleles (including a null allele) of βTub85D, though a deletion for αTub84B complements βTub85Dn. The genetic interaction between βTub85D and αTub84B is based on the structural interaction between the protein products of each gene.
    The expression of βTub60D is accompanied by a coordinate transient increase in the level of synthesis of the embryonic α-tubulins, thereby maintaining an approximately equimolar synthesis of α- and β-tubulins throughout embryogenesis.
    In D.melanogaster, two multigene families, each made up of four members, code for α- and β-tubulins. Tubulins are a highly conserved family of proteins that are the main structural components of microtubules in mitotic and meiotic spindles, cilia, flagella, neural processes and the cytoskeleton; nontubulin proteins (MAPS or microtubule-associated proteins) are involved along with tubulins in the formation of specialized microtubules (FBrf0045282; FBrf0046966). Transcribed in the oocyte and the embryo, reaching a maximum level 6-9 hr after the egg is laid (FBrf0037623). A variety of post-translational modifications of αTub84B protein found in all tissues, some of which are tissue-specific (FBrf0049502). The gene appears to be constitutively expressed, its functions being common to most cells. Maternal-effect as well as zygotic lethality is shown by the more severe alleles. In the less severe hypomorphic alleles, heterozygotes die late in pupal life or early in adult life, the adults showing head, thoracic, or abdominal defects, bristle abnormalities, leg tremors and sterility (FBrf0046004). Both meiosis and cleavage stage mitoses are severely affected by mutations that result in a substantial decrease in the αTub67C/αTub84B+αTub84D ratio, though an increase has little effect on meiosis while still disrupting mitotic spindle formation (FBrf0058107). Missense mutations may be male sterile when doubly heterozygous with missense allele of βTub85D (FBrf0050548).
    Origin and Etymology
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    External Crossreferences and Linkouts ( 62 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    Other crossreferences
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    modMine - A data warehouse for the modENCODE project
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    BioGRID - A database of protein and genetic interactions.
    Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
    Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyMine - An integrated database for Drosophila genomics
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (86)
    Reported As
    Symbol Synonym
    Alpha-Tubulin84B
    BEST:LD32507
    Tuba84B
    alpha-tub84B
    anon-EST:Liang-1.59
    anon-EST:Liang-2.30
    chr3R:2914276..2914416
    l(3)84Bd
    l(3)g3
    ms(3)nc33
    α-tub84B
    α-tubulin 84B
    αTub1
    Name Synonyms
    &agr-tubulin 84B
    Alpha-tubulin 84B
    Tubulin 1 α-chain
    alpha tubulin
    alpha-tubulin 84B
    tubulin 1α
    tubulin alpha1 promoter
    tubulin84B
    α tubulin 84B
    α-Tubulin 84B
    α-Tubulin84B
    α-tubulin
    (Girotra et al., 2017, Valzania et al., 2014, Delgehyr et al., 2012, Hughes et al., 2011, Bucciarelli et al., 2009, Kner et al., 2009, Liang et al., 2009, Zhang et al., 2009, Cheng et al., 2008, Colombié et al., 2008, Gao et al., 2008, Hallen et al., 2008, Holtzman et al., 2008, Hoyle et al., 2008, Popodi et al., 2008, Popodi et al., 2008, Silverman-Gavrila et al., 2008, Dyer et al., 2007, Griffis et al., 2007, Lee et al., 2007, Murakami et al., 2007, Murray and Saint, 2007, Ohlstein and Spradling, 2007, Pandey et al., 2007, Rebollo et al., 2007, Rodrigues-Martins et al., 2007, Sakaguchi and Steward, 2007, Wang et al., 2007, Brandt, 2006, Hawley and Gilliland, 2006, MacDonald et al., 2006, Mendjan et al., 2006, Rujano et al., 2006, Staudt et al., 2006, Verollet et al., 2006, Yu et al., 2006, Cullen et al., 2005, Jin et al., 2005, Neisch et al., 2005, Wilson, 2005, Cook et al., 2004, Cottam et al., 2004, Kwon and Scholey, 2004, Logarinho et al., 2004, Maiato et al., 2004, McHugh et al., 2004, Meignin et al., 2004, Moon and Hazelrigg, 2004, Roper and Brown, 2004, Betschinger et al., 2003, Fuse et al., 2003, Lee et al., 2003, Mathe et al., 2003, Somma et al., 2003, Fei et al., 2002, Mollinari et al., 2002, Savoian and Rieder, 2002, Bellaiche et al., 2001, Ben-Yaacov et al., 2001, Li et al., 2001, Loppin et al., 2001, Sampaio et al., 2001, Stevenson et al., 2001, Wakefield et al., 2001, Brent et al., 2000, Lange et al., 2000, Leismann et al., 2000, Lemos et al., 2000, Roos et al., 2000, Sibon et al., 2000, Walker et al., 2000, Cullen et al., 1999, Gho et al., 1999, Larkin et al., 1999, Megraw et al., 1999, Moutinho-Santos et al., 1999, Swan et al., 1999, Wilson, 1999, Wilson, 1999, Yu and Bienz, 1999, Endow and Komma, 1998, Helps et al., 1998, Herrmann et al., 1998, Kuchinke et al., 1998, Callaini and Riparbelli, 1996, Eaton et al., 1996, Li and Kaufman, 1996, Brill et al., 1995, Johansen et al., 1995, Callaini et al., 1994, Endow et al., 1994, Baker et al., 1993, Bendena et al., 1991, Yasuda et al., 1991, Wu et al., 1990)
    α-tubulin 1
    α-tubulin84B
    α1 tubulin
    α1-tubulin
    αTubulin84B
    Secondary FlyBase IDs
    • FBgn0046254
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    References (633)