α-tubulin, α tubulin, tubulin, α-Tub, Tub
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.49
There is only one protein coding transcript and one polypeptide associated with this gene
450 (aa); 55 (kD predicted)
The location of maternal and constitutive
α-tubulins is indistinguishable until ~8 hrs of development as determined
by staining with maternal α-tubulin-specific and general α-tubulin
antibodies. Studies with mutant embryos lacking zygotic α-tubulin show
that the constitutive form is more stable than the maternal form.
Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.
Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling (By similarity).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\αTub84D using the Feature Mapper tool.
localization of acetylated or glutamylated tubulin detected; found only in stabilized microtubules.
The constitutive αTub isotypes peak between 9 and 15 hours of embryogenesis. Levels of the constitutive isoforms remain strong in all tissues through embryogenesis, especially in the esophagus and in the epidermis at segment boundaries.
GBrowse - Visual display of RNA-Seq signalsView Dmel\αTub84D in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: αTub84D CG2512
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
Both meiosis and cleavage stage mitoses are severely affected by mutations that result in a substantial decrease in the αTub67C/αTub84B+αTub84D ratio, though an increase has little effect on meiosis while still disrupting mitotic spindle formation.
Total α-tubulin levels remain high throughout embryogenesis. Constitutive isotypes are more stable than maternal isotypes in most tissues.
In D.melanogaster, two multigene families, each made up of four members, code for α- and β-tubulins. Tubulins are a highly conserved family of proteins that are the main structural components of microtubules in mitotic and meiotic spindles, cilia, flagella, neural processes and the cytoskeleton; nontubulin proteins (MAPS or microtubule-associated proteins) are involved along with tubulins in the formation of specialized microtubules (FBrf0045282; FBrf0046966). αTub84D is coordinately expressed with αTub84B at most developmental stages with the exception of the adult stage where no αTub84D mRNA can be detected in males (FBrf0049502).