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Gene Dmel\αTub85E

General Information
Symbol	Dmel\αTub85E	Species	D. melanogaster
Name	α-Tubulin at 85E	Annotation symbol	CG9476
Feature type	protein_coding_gene	FlyBase ID	FBgn0003886
Gene Model Status	Current	Stock availability	2 publicly available
Also Known As	Tub, α-tubulin, α-Tub, α85E, α2, alphaTub85E
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	85E6-85E6	Sequence location	3R:5,556,943..5,558,972 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene α-Tubulin at 85E is referred to in FlyBase by the symbol Dmel\αTub85E (CG9476, FBgn0003886). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: structural constituent of cytoskeleton. There is experimental evidence that it is involved in the biological process: mitotic spindle assembly checkpoint; cellular process; mitotic spindle organization; centrosome duplication. 6 alleles are reported. The phenotypes of these alleles are annotated with: spermatid; wing. It has one annotated transcript and one annotated polypeptide. Protein features are: Alpha tubulin; Tubulin; Tubulin, C-terminal; Tubulin, conserved site; Tubulin/FtsZ, 2-layer sandwich domain; Tubulin/FtsZ, C-terminal; Tubulin/FtsZ, GTPase domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult testis. Comments on Affy2 ProbeSet: ProbeSet 1623910_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of alphaTub85E. Gene sequence location is 3R:5556943..5558972.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	αTub85E (α2t) A structural gene for α-Tubulin. Only α-Tubulin not expressed maternally. Transcripts present from 6-8 hr through adult. Protein found in support cells of chordotonal organs, developing muscles, and somatic component of the testis (Matthews, Miller, and Kaufman).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 85E6-85E6   Limits computationally determined from genome sequence between P{EP}CrcEP3006 and P{lacW}s2681
Experimentally Determined Cytological Location
Cytogenetic map Notes References 85E-85E   (determined by in situ hybridisation)   (Drosopoulou and Scouras, 1998) 85E6-85E10   (determined by in situ hybridisation)   (Natzle and McCarthy, 1984) 85E6-85E10   (determined by in situ hybridisation)   (Mischke and Pardue, 1982) 85E6-85E10   (determined by in situ hybridisation)   (Kalfayan and Wensink, 1981) 85E6-85E10   (determined by in situ hybridisation)   (Sanchez et al., 1980)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\αTub85E for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46 Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) αTub85E-RA FBtr0082087  NM_079573   1560   449
Additional Transcript Data & Comments
Reported size (kB)	2.049 (sequence analysis) (Theurkauf et al., 1986) 2.0, 1.8 (northern blot) (Natzle and McCarthy, 1984) 1.85, 1.65 (northern blot) (Kalfayan and Wensink, 1982) 1.55 (northern blot) (Matthews et al., 1989)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein αTub85E-PA   FBpp0081565   50.0   449   4.83     NP_524297   AAF54433
Additional Polypeptide Data & Comments
Reported size (kDa)	449 (aa); 55 (kD predicted) (Theurkauf et al., 1986)
Comments	Polypeptides were identified in wing imaginal discs and in the CME W2 wing imaginal disc cell line by 2D gel electrophoresis and by microsequencing. (Santaren et al., 1993)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Tubulin (IPR000217) Alpha tubulin (IPR002452) Tubulin/FtsZ, GTPase domain (IPR003008) Tubulin/FtsZ, C-terminal (IPR008280) Tubulin, conserved site (IPR017975) Tubulin/FtsZ, 2-layer sandwich domain (IPR018316) Tubulin, C-terminal (IPR023123)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA979650   AE014297 AAF54433   AI295451   AI944857   AI946623   AK278735   AK281777   AK282586   AY084208   AY113631 AAM29636   AY231787   AY432371   BE978409   BF485571   BF489482   BF495372   BF495746   BF497424   BF500552   BF503401   BF504060   BI165568   BI167088   BI213816   BI236732   BI239011   BI240461   BI240899   BI240922   BI240981   BI240983   BI356315   BI368579   BI374066   BI374963   BI482869   BI578815   BI580417   BI605864   BT003630   BT012506   BT016118   BT044334   BX005570   BX006068   BX006881   BX007023   BX007757   BX007996   BX009126   BX009302   BX011538   BX014125   BX015446   BX015701   BX016319   BX016721   BX017052   BX018244   BX019574   BX020136   BX020289   BX020626   BX020651   BX020705   BX021167   BX022058   BX022214   BX023695   BX024048   BX024443   BX026450   BX026755   BX026827   BX028325   BX029668   BX029685   BX029728   BX030828   BX031517   BX032230   BX033080   BX034162   BX034268   BX034454   BX037080   BX038833   BX041328   BX043013   BX044536   BX047470   BX050483   BX051458   BX053542   BX054934   BX056074   BX056628   BX057344   BX060127   BX061026   BX061199   BX062157   BX063462   BX064928   BX065134   BX066139   BX067109   BX067643   BX068310   BX069757   BX070179   BX071989   BX072076   CO312800   CR938635   DQ440241   DQ675550   EC065044   EF207983   GH749019   GH749596   GH751617   GH771349   GH772029   GH772223   GH774730   GH776376   GH776821   GH778163   GH780804   GH780881   GH781132   GH781519   GH783734   GH784451   GH788392   GH789296   GH789685   GH790054   GH792512   GH794792   GH794801   GH794866   GH799053   GH799203   GH799635   GH801959   GH802197   GH803254   GH803293   GH803307   GH803912   GH808435   GH810048   GH811385   GH816497   GH816782   GH817191   GH817698   GH818080   GH821050   GH821821   GH822346   GH822889   GH824944   GH825064   GH826344   GH828876   GH829447   GH832364   GH832422   GH833191   GH833802   GH834034   GH835391   GH839277   GH841878   GH842499   GH842982   GH843672   GH843958   GH846182   GH846684   GH847202   GH847372   GH849578   GH849679   GH853535   GH855184   GH855699   GH858155   GH858247   GH858989   GH859615   GH861317   GH861579   GH861905   GH861937   GH863407   GH865572   GH866193   GH866787   GH867033   GH867264   GH869614   GH870299   GH871090   GH871584   GH874747   GH874810   GH874872   GH876672   GH876916   GH878410   GH880509   GH882696   GH885169   GH891486   GH892872   GH893601   GH894177   GH895149   GH895414   GH895749   GH897079   GH898479   GH900460   GH903714   GH904547   GH904728   GH905298   GH905746   GH907928   GH908769   GH909318   GH909749   GH909829   GH910117   GH910956   GH911864   GH912860   GH915000   GH915265   GH917491   GH918167   GH918856   GH919229   GH920223   GH920230   GH921216   GH924174   GH929577   GH930783   GH931101   GH932157   GH935123   GH936835   GH938945   GH940515   GH940814   GH941090   M14644 AAA28986
UniProtKB/Swiss-Prot	P06604
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference   organism   (Matthews et al., 1989) Comment:reference states 8-12 hr AEL in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic/larval somatic muscle   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 11     (Natzle and McCarthy, 1984) Comment:reference states 0-6 hr AEL embryonic stage 4 -- 17     (Natzle and McCarthy, 1984) Comment:reference states 2-21 hr AEL embryonic stage 11 -- 15     (Kalfayan and Wensink, 1982) Comment:reference states 6-12 hr AEL larval stage     (Kalfayan and Wensink, 1982) larval stage -- pupal stage     (Matthews et al., 1989) first instar larval stage     (Natzle and McCarthy, 1984) second instar larval stage     (Natzle and McCarthy, 1984) third instar larval stage     (Natzle and McCarthy, 1984) prepupal stage     (Natzle and McCarthy, 1984) pupal stage     (Kalfayan and Wensink, 1982, Natzle and McCarthy, 1984) adult stage | male     (Kalfayan and Wensink, 1982) spermatogenesis & adult stage   testis   (Matthews et al., 1989)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   wing disc   (Santaren et al., 1993) immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 13 -- first instar larval stage   scolopidial dendritic cap cell | subset   (Inbal et al., 2004) first instar larval stage   scolopidial ligament cell | subset   (Inbal et al., 2004) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 17     (Matthews et al., 1989) Comment:reference states 16-20 hr AEL larval stage   imaginal disc   (Matthews et al., 1989) spermatogenesis & adult stage   testis   (Matthews et al., 1989)
Additional Descriptive Data
	To compare synthesis and accumulation of α-tubulin proteins, 35S</up>methionine radiolabeled tissues were subjected to Western analysis using monoclonal anti-α-tubulin antibody. Newly synthesized αTub85E protein was observed possibly in testes and imaginal disc tissue, but a stable pool was observed only in late embryos. (Matthews et al., 1989)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    5.175 Larval Midgut    2.2 Larval Hindgut    24.6 Larval Malpighian Tubules    4.2 Larval Fat Body    72.3 Larval Salivary Gland    27.1 Larval Trachea    43 Larval Carcass    512.15 Adult Head    7.1 Adult Eye    3.6 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    10.1 Adult Crop    11.9 Adult Midgut    2.5 Adult Hindgut    2.5 Adult Malpighian Tubules    2.8 Adult Fat Body    8.1 Adult Salivary Gland    8.4 Adult Heart    1.825 Adult VirginFemale Spermatheca    6.8 Adult InseminatedFemale Spermatheca    3.9 Adult Ovary    4.9 Adult Testis    361.6 Adult Male Accessory Gland    10.1 Adult Carcass    53.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    5.175 Larval Midgut    2.2 Larval Hindgut    24.6 Larval Malpighian Tubules    4.2 Larval Fat Body    72.3 Larval Salivary Gland    27.1 Larval Trachea    43 Larval Carcass  512.15 Adult Head    7.1 Adult Eye    3.6 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    10.1 Adult Crop    11.9 Adult Midgut    2.5 Adult Hindgut    2.5 Adult Malpighian Tubules    2.8 Adult Fat Body    8.1 Adult Salivary Gland    8.4 Adult Heart    1.825 Adult VirginFemale Spermatheca    6.8 Adult InseminatedFemale Spermatheca    3.9 Adult Ovary    4.9 Adult Testis    361.6 Adult Male Accessory Gland    10.1 Adult Carcass    53.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    5.175 Larval Midgut    2.2 Larval Hindgut    24.6 Larval Malpighian Tubules    4.2 Larval Fat Body    72.3 Larval Salivary Gland    27.1 Larval Trachea    43 Larval Carcass    512.15 Adult Head    7.1 Adult Eye    3.6 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    10.1 Adult Crop    11.9 Adult Midgut    2.5 Adult Hindgut    2.5 Adult Malpighian Tubules    2.8 Adult Fat Body    8.1 Adult Salivary Gland    8.4 Adult Heart    1.825 Adult VirginFemale Spermatheca    6.8 Adult InseminatedFemale Spermatheca    3.9 Adult Ovary    4.9 Adult Testis    361.6 Adult Male Accessory Gland    10.1 Adult Carcass    53.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    5.175 Larval Midgut    2.2 Larval Hindgut    24.6 Larval Malpighian Tubules    4.2 Larval Fat Body    72.3 Larval Salivary Gland    27.1 Larval Trachea    43 Larval Carcass    512.15 Adult Head    7.1 Adult Eye    3.6 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    10.1 Adult Crop    11.9 Adult Midgut    2.5 Adult Hindgut    2.5 Adult Malpighian Tubules    2.8 Adult Fat Body    8.1 Adult Salivary Gland    8.4 Adult Heart    1.825 Adult VirginFemale Spermatheca    6.8 Adult InseminatedFemale Spermatheca    3.9 Adult Ovary    4.9 Adult Testis    361.6 Adult Male Accessory Gland    10.1 Adult Carcass    53.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    5.175 Larval Midgut    2.2 Larval Hindgut    24.6 Larval Malpighian Tubules    4.2 Larval Fat Body    72.3 Larval Salivary Gland    27.1 Larval Trachea    43 Larval Carcass    512.15 Adult Head    7.1 Adult Eye    3.6 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    10.1 Adult Crop    11.9 Adult Midgut    2.5 Adult Hindgut    2.5 Adult Malpighian Tubules    2.8 Adult Fat Body    8.1 Adult Salivary Gland    8.4 Adult Heart    1.825 Adult VirginFemale Spermatheca    6.8 Adult InseminatedFemale Spermatheca    3.9 Adult Ovary    4.9 Adult Testis    361.6 Adult Male Accessory Gland    10.1 Adult Carcass    53.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    5.175 Larval Midgut    2.2 Larval Hindgut    24.6 Larval Malpighian Tubules    4.2 Larval Fat Body    72.3 Larval Salivary Gland    27.1 Larval Trachea    43 Larval Carcass  512.15 Adult Head    7.1 Adult Eye    3.6 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    10.1 Adult Crop    11.9 Adult Midgut    2.5 Adult Hindgut    2.5 Adult Malpighian Tubules    2.8 Adult Fat Body    8.1 Adult Salivary Gland    8.4 Adult Heart    1.825 Adult VirginFemale Spermatheca    6.8 Adult InseminatedFemale Spermatheca    3.9 Adult Ovary    4.9 Adult Testis    361.6 Adult Male Accessory Gland    10.1 Adult Carcass    53.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    5.175 Larval Midgut    2.2 Larval Hindgut    24.6 Larval Malpighian Tubules    4.2 Larval Fat Body    72.3 Larval Salivary Gland    27.1 Larval Trachea    43 Larval Carcass    512.15 Adult Head    7.1 Adult Eye    3.6 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    10.1 Adult Crop    11.9 Adult Midgut    2.5 Adult Hindgut    2.5 Adult Malpighian Tubules    2.8 Adult Fat Body    8.1 Adult Salivary Gland    8.4 Adult Heart    1.825 Adult VirginFemale Spermatheca    6.8 Adult InseminatedFemale Spermatheca    3.9 Adult Ovary    4.9 Adult Testis    361.6 Adult Male Accessory Gland    10.1 Adult Carcass    53.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    5.175 Larval Midgut    2.2 Larval Hindgut    24.6 Larval Malpighian Tubules    4.2 Larval Fat Body    72.3 Larval Salivary Gland    27.1 Larval Trachea    43 Larval Carcass    512.15 Adult Head    7.1 Adult Eye    3.6 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    10.1 Adult Crop    11.9 Adult Midgut    2.5 Adult Hindgut    2.5 Adult Malpighian Tubules    2.8 Adult Fat Body    8.1 Adult Salivary Gland    8.4 Adult Heart    1.825 Adult VirginFemale Spermatheca    6.8 Adult InseminatedFemale Spermatheca    3.9 Adult Ovary    4.9 Adult Testis    361.6 Adult Male Accessory Gland    10.1 Adult Carcass    53.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    7.1   NA  Eye    3.6   NA  Brain    4.1   5.175  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    no informative data   2.2  Midgut    2.5   no informative data  Hindgut    2.5   4.2  Malpighian Tubules    2.8   no informative data  Fat Body    8.1   no informative data  Salivary Gland    8.4   NA  Heart    1.825   43  Trachea    NA   NA  VirginFemale Spermatheca    6.8   NA  InseminatedFemale Spermatheca    3.9   NA  Ovary    4.9   NA  Testis    361.6   NA  Male Accessory Gland    no informative data   512.15  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    23 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8    51 carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    138 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    23 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8  (51) carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male  (138) accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    23 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8    51 carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male  (138) accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    23 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8    51 carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    138 accessory gland, mated 4-day male    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    23 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8    51 carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    138 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    23 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8  (51) carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male  (138) accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    23 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8    51 carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male  138 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    23 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8    51 carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    138 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    5 embryo 08-10hr    10 embryo 10-12hr    71 embryo 12-14hr    70 embryo 14-16hr    149 embryo 16-18hr    132 embryo 18-20hr    166 embryo 20-22hr    134 embryo 22-24hr    112 larva L1    139 larva L2    118 larva L3 12hr old    80 larva L3 puffstage 1-2    32 larva L3 puffstage 3-6    40 larva L3 puffstage 7-9    38 white prepupae new    31 white prepupae 12hr    40 white prepupae 24hr    150 pupae 2d postWPP    88 pupae 3d postWPP    65 pupae 4d postWPP    47 adult male 01day    39 adult male 05day    36 adult male 30day    36 adult female 01day    6 adult female 05day    1 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    5 embryo 08-10hr    10 embryo 10-12hr  (71) embryo 12-14hr  (70) embryo 14-16hr  (149) embryo 16-18hr  (132) embryo 18-20hr  (166) embryo 20-22hr  (134) embryo 22-24hr  (112) larva L1  (139) larva L2  (118) larva L3 12hr old  (80) larva L3 puffstage 1-2  (32) larva L3 puffstage 3-6  (40) larva L3 puffstage 7-9  (38) white prepupae new  (31) white prepupae 12hr  (40) white prepupae 24hr  (150) pupae 2d postWPP  (88) pupae 3d postWPP  (65) pupae 4d postWPP  (47) adult male 01day  (39) adult male 05day  (36) adult male 30day  (36) adult female 01day    6 adult female 05day    1 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    5 embryo 08-10hr    10 embryo 10-12hr    71 embryo 12-14hr    70 embryo 14-16hr  (149) embryo 16-18hr  (132) embryo 18-20hr  (166) embryo 20-22hr  (134) embryo 22-24hr  (112) larva L1  (139) larva L2  (118) larva L3 12hr old    80 larva L3 puffstage 1-2    32 larva L3 puffstage 3-6    40 larva L3 puffstage 7-9    38 white prepupae new    31 white prepupae 12hr    40 white prepupae 24hr  (150) pupae 2d postWPP    88 pupae 3d postWPP    65 pupae 4d postWPP    47 adult male 01day    39 adult male 05day    36 adult male 30day    36 adult female 01day    6 adult female 05day    1 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    5 embryo 08-10hr    10 embryo 10-12hr    71 embryo 12-14hr    70 embryo 14-16hr    149 embryo 16-18hr    132 embryo 18-20hr    166 embryo 20-22hr    134 embryo 22-24hr    112 larva L1    139 larva L2    118 larva L3 12hr old    80 larva L3 puffstage 1-2    32 larva L3 puffstage 3-6    40 larva L3 puffstage 7-9    38 white prepupae new    31 white prepupae 12hr    40 white prepupae 24hr    150 pupae 2d postWPP    88 pupae 3d postWPP    65 pupae 4d postWPP    47 adult male 01day    39 adult male 05day    36 adult male 30day    36 adult female 01day    6 adult female 05day    1 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    5 embryo 08-10hr    10 embryo 10-12hr    71 embryo 12-14hr    70 embryo 14-16hr    149 embryo 16-18hr    132 embryo 18-20hr    166 embryo 20-22hr    134 embryo 22-24hr    112 larva L1    139 larva L2    118 larva L3 12hr old    80 larva L3 puffstage 1-2    32 larva L3 puffstage 3-6    40 larva L3 puffstage 7-9    38 white prepupae new    31 white prepupae 12hr    40 white prepupae 24hr    150 pupae 2d postWPP    88 pupae 3d postWPP    65 pupae 4d postWPP    47 adult male 01day    39 adult male 05day    36 adult male 30day    36 adult female 01day    6 adult female 05day    1 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    5 embryo 08-10hr    10 embryo 10-12hr  (71) embryo 12-14hr  (70) embryo 14-16hr  (149) embryo 16-18hr  (132) embryo 18-20hr  (166) embryo 20-22hr  (134) embryo 22-24hr  (112) larva L1  (139) larva L2  (118) larva L3 12hr old  (80) larva L3 puffstage 1-2  32 larva L3 puffstage 3-6  (40) larva L3 puffstage 7-9  (38) white prepupae new  31 white prepupae 12hr  (40) white prepupae 24hr  (150) pupae 2d postWPP  (88) pupae 3d postWPP  (65) pupae 4d postWPP  (47) adult male 01day  (39) adult male 05day  (36) adult male 30day  (36) adult female 01day    6 adult female 05day    1 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    5 embryo 08-10hr    10 embryo 10-12hr    71 embryo 12-14hr    70 embryo 14-16hr  (149) embryo 16-18hr  132 embryo 18-20hr  (166) embryo 20-22hr  134 embryo 22-24hr  112 larva L1  139 larva L2  118 larva L3 12hr old    80 larva L3 puffstage 1-2    32 larva L3 puffstage 3-6    40 larva L3 puffstage 7-9    38 white prepupae new    31 white prepupae 12hr    40 white prepupae 24hr  (150) pupae 2d postWPP    88 pupae 3d postWPP    65 pupae 4d postWPP    47 adult male 01day    39 adult male 05day    36 adult male 30day    36 adult female 01day    6 adult female 05day    1 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    5 embryo 08-10hr    10 embryo 10-12hr    71 embryo 12-14hr    70 embryo 14-16hr    149 embryo 16-18hr    132 embryo 18-20hr    166 embryo 20-22hr    134 embryo 22-24hr    112 larva L1    139 larva L2    118 larva L3 12hr old    80 larva L3 puffstage 1-2    32 larva L3 puffstage 3-6    40 larva L3 puffstage 7-9    38 white prepupae new    31 white prepupae 12hr    40 white prepupae 24hr    150 pupae 2d postWPP    88 pupae 3d postWPP    65 pupae 4d postWPP    47 adult male 01day    39 adult male 05day    36 adult male 30day    36 adult female 01day    6 adult female 05day    1 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    9 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    17 embryonic S1    1 embryonic S3    1 leg disc CME L1    16 wing disc CME-W2    28 wing disc ML-DmD8    9 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    9 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    17 embryonic S1    1 embryonic S3    1 leg disc CME L1    16 wing disc CME-W2  (28) wing disc ML-DmD8    9 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    9 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    17 embryonic S1    1 embryonic S3    1 leg disc CME L1    16 wing disc CME-W2    28 wing disc ML-DmD8    9 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    9 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    17 embryonic S1    1 embryonic S3    1 leg disc CME L1    16 wing disc CME-W2    28 wing disc ML-DmD8    9 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    9 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    17 embryonic S1    1 embryonic S3    1 leg disc CME L1    16 wing disc CME-W2    28 wing disc ML-DmD8    9 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    9 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    17 embryonic S1    1 embryonic S3    1 leg disc CME L1    16 wing disc CME-W2  28 wing disc ML-DmD8    9 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    9 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    17 embryonic S1    1 embryonic S3    1 leg disc CME L1    16 wing disc CME-W2    28 wing disc ML-DmD8    9 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    9 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    17 embryonic S1    1 embryonic S3    1 leg disc CME L1    16 wing disc CME-W2    28 wing disc ML-DmD8    9 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    1 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    29 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    34 Ethanol 10% 3 hrs, larvae L3    28 Caffeine 1.5 mg/ml 4 hrs, larvae L3    31 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3    30 Rotenone 8 μg 12 hrs, larvae L3    38 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3  (29) Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3  (34) Ethanol 10% 3 hrs, larvae L3  (28) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (31) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3  (30) Rotenone 8 μg 12 hrs, larvae L3  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    29 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    34 Ethanol 10% 3 hrs, larvae L3    28 Caffeine 1.5 mg/ml 4 hrs, larvae L3    31 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3    30 Rotenone 8 μg 12 hrs, larvae L3    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    29 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    34 Ethanol 10% 3 hrs, larvae L3    28 Caffeine 1.5 mg/ml 4 hrs, larvae L3    31 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3    30 Rotenone 8 μg 12 hrs, larvae L3    38 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    29 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    34 Ethanol 10% 3 hrs, larvae L3    28 Caffeine 1.5 mg/ml 4 hrs, larvae L3    31 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3    30 Rotenone 8 μg 12 hrs, larvae L3    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3  29 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3  (34) Ethanol 10% 3 hrs, larvae L3  28 Caffeine 1.5 mg/ml 4 hrs, larvae L3  31 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3  30 Rotenone 8 μg 12 hrs, larvae L3  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    29 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    34 Ethanol 10% 3 hrs, larvae L3    28 Caffeine 1.5 mg/ml 4 hrs, larvae L3    31 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3    30 Rotenone 8 μg 12 hrs, larvae L3    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    29 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    34 Ethanol 10% 3 hrs, larvae L3    28 Caffeine 1.5 mg/ml 4 hrs, larvae L3    31 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3    30 Rotenone 8 μg 12 hrs, larvae L3    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_29 mE1_20_mRNA_expression_cluster_15
External Data & Images
Linkouts	BDGP expression data • FI07460 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003886 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9476-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Sterility Allele male fertile αTub85EβTub85D.PH Other Phenotypes Allele visible, with Scer\GAL4Bx-MS1096 αTub85EHM04009 Phenotype manifest in Allele spermatid αTub85EβTub85D.PH wing, with Scer\GAL4Bx-MS1096 αTub85EHM04009
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of αTub85E Class Mutagen Stocks Known lesion αTub85Eunspecified 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of αTub85E Class Mutagen Stocks Known lesion αTub85EHM04009 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes αTub85EKK109599 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes αTub85EGD14226 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes αTub85EHMS02598 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes αTub85EβTub85D.PH in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)by10 (Seshaiah et al., 2001) Df(3R)GB104 (Seshaiah et al., 2001) Tp(3;2)by62 (Seshaiah et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data reporter construct P{Tα2-lacZ.1} No P{Tα2-lacZ.2} No
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cellular process inferred from mutant phenotype (Boutros et al., 2004) centrosome duplication inferred from mutant phenotype (Müller et al., 2010) mitotic spindle assembly checkpoint inferred from direct assay (Logarinho et al., 2004) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
GTPase activity inferred from electronic annotation with InterPro:IPR008280, InterPro:IPR018316 (FlyBase Curators et al., 2004-) GTP binding inferred from electronic annotation with InterPro:IPR000217, InterPro:IPR002452, InterPro:IPR008280, InterPro:IPR017975, InterPro:IPR018316 (FlyBase Curators et al., 2004-) non-traceable author statement (Swiss-Prot Project Members, 1988.1.1) structural constituent of cytoskeleton inferred from electronic annotation with InterPro:IPR002452 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
Biological Process
CV term Evidence References
GTP catabolic process inferred from electronic annotation with InterPro:IPR008280, InterPro:IPR018316 (FlyBase Curators et al., 2004-) protein polymerization inferred from electronic annotation with InterPro:IPR003008, InterPro:IPR008280, InterPro:IPR018316 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
microtubule inferred from electronic annotation with InterPro:IPR000217, InterPro:IPR002452, InterPro:IPR017975 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) non-traceable author statement (Swiss-Prot Project Members, 1988.1.1) tubulin complex non-traceable author statement (Swiss-Prot Project Members, 1988.1.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions αTub85E allele Gene References αTub85EβTub85D.PH βTub85D (Hoyle et al., 2001)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0003886 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41183
Orthologs
OrthoDB Orthologs (54) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG69W316 Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search EOG6CK79F
Orthologs in Drosophila Species (EOG69W316)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\αTub85E     Drosophila simulans   Dsim\GD20767 Y   Drosophila sechellia   Dsec\GM26223 Y   Drosophila erecta   Dere\GG17337 Y   Drosophila yakuba   Dyak\GE24741 Y   Drosophila ananassae   Dana\GF11529 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (EOG6CK79F)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\tba-4 Y Caenorhabditis elegans Nematode  Cele\mec-12 Y Caenorhabditis elegans Nematode  Cele\tba-1 Y Caenorhabditis elegans Nematode  Cele\tba-9 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_004143gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_021668gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_019989gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_007984gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_021669gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_019990gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_012679gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024615gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016746gn Y Danio rerio Zebrafish  Drer\tuba4l Y Danio rerio Zebrafish  Drer\tuba1l Y Danio rerio Zebrafish  Drer\OTTDARG00000032770 Y Danio rerio Zebrafish  Drer\tuba2 Y Danio rerio Zebrafish  Drer\tuba1l2 Y Danio rerio Zebrafish  Drer\tuba8l4 Y Danio rerio Zebrafish  Drer\tuba8l2 Y Danio rerio Zebrafish  Drer\tuba8l Y Danio rerio Zebrafish  Drer\tuba1 Y Danio rerio Zebrafish  Drer\tuba8l3 Y Danio rerio Zebrafish  Drer\tuba7l Y Xenopus tropicalis Western clawed frog  Xtro\tuba4b Y Xenopus tropicalis Western clawed frog  Xtro\tuba1b Y Xenopus tropicalis Western clawed frog  Xtro\tuba4a Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000019254 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000019246 Y Xenopus tropicalis Western clawed frog  Xtro\tuba3c Y Gallus gallus Domestic chicken  Ggal\ENSGALG00000022081 Y Gallus gallus Domestic chicken  Ggal\TUBA4A Y Gallus gallus Domestic chicken  Ggal\ENSGALG00000011324 Y Gallus gallus Domestic chicken  Ggal\TUBA3E Y Gallus gallus Domestic chicken  Ggal\LOC424201 Y Gallus gallus Domestic chicken  Ggal\TUBA3E Y Mus musculus House mouse  Mmus\Tuba8 Y Mus musculus House mouse  Mmus\Tuba1b Y Mus musculus House mouse  Mmus\Tuba1a Y Mus musculus House mouse  Mmus\Tuba4a Y Rattus norvegicus Norway rat  Rnor\Tuba1a Y Rattus norvegicus Norway rat  Rnor\Tuba1b Y Rattus norvegicus Norway rat  Rnor\Tuba4a Y Homo sapiens Human  Hsap\TUBA2 Y Homo sapiens Human  Hsap\TUBA2 Y Homo sapiens Human  Hsap\TUBA4A Y Homo sapiens Human  Hsap\TUBA1C Y Homo sapiens Human  Hsap\TUBA3E Y
Human Orthologs (5)
Gene OMIM HGNC Hsap\TUBA2     Hsap\TUBA2     Hsap\TUBA4A     Hsap\TUBA1C     Hsap\TUBA3E
AAA Orthologs (6) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD20767 Drosophila sechellia Dsec\GM26223 Drosophila erecta Dere\GG17337 Drosophila yakuba Dyak\GE24741 Drosophila ananassae Dana\GF11529 Drosophila mojavensis Dmoj\GI23620
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
Bloomington	31703 y1 v1; P{TRiP.HM04009}attP2
VDRC	v103202 P{KK109599}VIE-260B
Genomic Clones ( 2 )
	BACR23O04 BACR30F01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 178 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT25469 AT26363 LD07757 RH71862 SD07763
Other clones	FI07460 yak-em_alphaTub84B
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT04207 AT05341 AT09392 AT11589 AT15535 AT19377 AT19611 AT25436 RE05488 RE07384 RE19425 RE32839 RE35390 RE37310 RE37856 RE37881 RE37950 RE37952 RE42430 RE54572 RE61578 RE62660 RE65657 RE72744 RE74610
Other clones	bs06c05 bs28h03 bs78d07 EK229987 RP003036370 RP003037110 RP003043364 RP003046276 RP003059319 RP003063709 RP003067221 RP003068767 RP003071426 RP003073335 RP003074961 RP003087155 RP003087918 RP003088227 RP003097436 RP003101992 RP003103838 RP003103902 RP003115741 RP003118720 RP003120683 RP003122603 RP003129829 RP003130213 RP003134904 RP003135973 RP003146366 RP003149199 RP003151288 RP003157867 RP003158849 RP003159328 RP003159776 RP003163787 RP003175554 RP003175584 RP003176614 RP003182294 RP003183718 RP003186955 RP003193454 RP003194954 RP003199190 RP003201905 RP003202456 RP003202502 RP003204618 RP003211442 RP003224148 RP003228390 RP003228579 RP003231642 RP003233190 RP003236734 RP003240840 RP003251293 RP003263175 RP003265545 RP003266693 RP003268901 RP003272266 RP003274311 RP003278150 RP003279464 RP003282422 RP003282655 RP003283028 RP003286970 RP003292827 RP003293981 RP003296851 RP003298479 RP003298511 RP003309979 RP003312224 RP003316646 RP003331044 RP003331855 RP003332675 RP003342080 RP003346844 RP003348811 RP003349525 RP003352163 RP003392793 RP003394228 RP003402272 RP003408333 RP003421264 RP003425639 RP003431032 RP003451230 RP003457731 RP003459119 RP003462197 RP003464808 RP003464831 RP003470550 RP003478964 RP003479624 RP003484079 RP003504050 RP003516950 RP003521879 RP003532774 RP003543171 RP003544190 RP003552134 RP003555964 RP003556787 RP003556932 RP003571526 RP003586526 RP003587454 RP003588016 RP003591857 RP003604269 RP003605774 RP003610647 RP003616858 RP003617751 RP003624108 RP003626035 RP003629525 RP003629940 RP003635675 RP003636476 RP003640976 RP003642338 RP003650673 RP003654970 RP003655323 RP003655394 RP003662009 RP003664677 RP003665428 RP003667382 RP003669747 RP003672460 RP003672531 RP003673469 RP003674328
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003886 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41183
Antibody Information
	polyclonal (Klein et al., 2010)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene in S2 cell results in dim staining of microtubules, and a short monopolar spindle. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection acting against preferred codons at the start of genes. (Kliman and Eyre-Walker, 1998) Though αTub84B and αTub85E are very similar in sequence they are functionally distinct. αTub85E can provide function in the male germ line fr generic microtubule arrays but lacks a subset of the functional properties possessed by necessary for specific features of microtubule assembly unique to the male germ cells. (Hutchens et al., 1997) Identified in 2D gels of CMW W2 wing imaginal disc cell proteins. (Santaren et al., 1993) The tissue specific pattern of αTub85E protein accumulation is identified by isotope specific antibody. (Matthews et al., 1990) The expression of βTub60D is accompanied by a coordinate transient increase in the level of synthesis of the embryonic α-tubulins, thereby maintaining an approximately equimolar synthesis of α- and β-tubulins throughout embryogenesis. (Raff et al., 1982) In D.melanogaster, two multigene families, each made up of four members, code for α- and β-tubulins. Tubulins are a highly conserved family of proteins that are the main structural components of microtubules in mitotic and meiotic spindles, cilia, flagella, neural processes and the cytoskeleton; nontubulin proteins (MAPS or microtubule-associated proteins) are involved along with tubulins in the formation of specialized microtubules (FBrf0045282; FBrf0046966). αTub85E is the only α-tubulin not expressed maternally. Transcripts present from 6-8 hr through adult. Protein found in support cells of chordotonal organs, developing muscles and somatic component of the testis (FBrf0049502). In adults expression of αTub85E is male-specific (FBrf0045282).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079573 RefSeq (Proteins) • NP_524297 DDBJ / EMBL / GenBank • AA979650 AAA28986 AAF54433 AAM29636 AI295451 AI944857 AI946623 AY113631 BI605864 M14644 Entrez Gene - A searchable database of RefSeq genes. • 41183 UniProtKB/Swiss-Prot • P06604
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Tubulin (IPR000217) Alpha tubulin (IPR002452) Tubulin/FtsZ, GTPase domain (IPR003008) Tubulin/FtsZ, C-terminal (IPR008280) Tubulin, conserved site (IPR017975) Tubulin/FtsZ, 2-layer sandwich domain (IPR018316) Tubulin, C-terminal (IPR023123) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • FI07460
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0003886 DRSC - Results from RNAi screens. • FBgn0003886 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003886 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9476-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003886 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41183 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41183 modMine - Data generated by the modENCODE project. • FBgn0003886
Synonyms & Secondary IDs ( 34 )
Reported As
Symbol Synonym	2t (Fyrberg and Goldstein, 1990) a2t (Fuller, 1993) ALPHA 85E (Goldstein and Gunawardena, 2000) alphaTub85E (Friedman et al., 2011, Müller et al., 2010) aTub85E (Kliman and Eyre-Walker, 1998) CG9476 (Goldstein and Gunawardena, 2000, Goshima et al., 2007, Keleman et al., 2009.8.5, Wertheim et al., 2005, Ni et al., 2008, Ni et al., 2010.12.1) DTA3 (Natzle and McCarthy, 1984) T (Courgeon et al., 1993) Tub (Wei et al., 2004, Harris and Peifer, 2004, Tokusumi et al., 2012) α2 (Drosopoulou and Scouras, 1998, Bo and Wensink, 1989) α2t   α85E (Hoyle et al., 2001, Raff, 1984, Nielsen et al., 2006) α85E-tub (Inbal et al., 2004) α-Tub (Ruel et al., 2003, Fichelson et al., 2010) αTub (Audibert et al., 2005) α-tub (Delgehyr et al., 2012) αTub2 (Mansfield et al., 1994, ) αTub84E (Moritz, 1993) α-Tub85E (Klein et al., 2010) αTub85E (Goshima et al., 2007, Weake et al., 2011, Wertheim et al., 2005, Sharma et al., 2011) α-tubulin (Martinez-Campos et al., 2004, Wasser and Chia, 2003, Maiato et al., 2006)
Name Synonym	alpha-tubulin (Przewloka et al., 2007, Siudeja et al., 2011) alpha tubulin (Nielsen et al., 2010) tubulin (Harris and Peifer, 2004, Galindo et al., 2007, Harris and Peifer, 2005, Goshima et al., 2005, Ashley et al., 2005, Yuen et al., 2008) α2-tubulin (Bo and Wensink, 1989) α2tubulin (Raff et al., 1982) α85E-tubulin (Dettman et al., 2001) αT (Galindo et al., 2007) α-Tubulin (Wei et al., 2004, Betschinger et al., 2005, Berdnik and Knoblich, 2002, van Steensel et al., 2001, Brandt et al., 2006, Szafranski and Goode, 2007, Fichelson et al., 2010) α-tubulin (Kwon and Scholey, 2004, Cottam et al., 2004, Fuse et al., 2003, Betschinger et al., 2003, Mollinari et al., 2002, Sampaio et al., 2001, Bellaiche et al., 2001, Wakefield et al., 2001, Walker et al., 2000, Ben-Yaacov et al., 2001, Leismann et al., 2000, Roos et al., 2000, Lemos et al., 2000, Lange et al., 2000, Wilson, 1999, Wilson, 1999, Gho et al., 1999, Herrmann et al., 1998, Kuchinke et al., 1998, Riparbelli et al., 1997, Eaton et al., 1996, Brill et al., 1995, Endow et al., 1994, Callaini et al., 1994, Baker et al., 1993, Wu et al., 1990, Logarinho et al., 2004, Cenci et al., 2003, Somma et al., 2003, Roper and Brown, 2004, Mendjan et al., 2006, Verollet et al., 2006, Goshima et al., 2007, Wilson, 2005, McHugh et al., 2004, Murray and Saint, 2007, Ohlstein and Spradling, 2007, Rujano et al., 2006, Sakaguchi and Steward, 2007, Meignin et al., 2004, Cullen et al., 2005, Cheng et al., 2008, Holtzman et al., 2008, Gao et al., 2008, Wang et al., 2007, Zhang et al., 2009, Kner et al., 2009, Brandt, 2006, Bucciarelli et al., 2009, Delgehyr et al., 2012) αtubulin (Battaglia et al., 2001, Grieder and Spradling, 1999) α tubulin (Donaldson et al., 2001, Audibert and Simonelig, 1999, Nicolai et al., 1999, Avides and Glover, 1999, Bonaccorsi et al., 1998, Abdu et al., 2006) α-Tubulin 85E (O'Farrell and Kylsten, 2008) α-tubulin 85E (Cachero et al., 2011) α-Tubulin at 85E
Secondary FlyBase IDs
References ( 160 )
Generate a list of	All references relating to this gene ( 160 )
List References by type	Research paper  ( 125 ) Supplementary material  ( 9 ) Review  ( 8 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 6 ) Letter  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 ) Book  ( 1 )
Recent research papers ( 8 )
	Delgehyr et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(15): 5729--5734 Drosophila Mgr, a Prefoldin subunit cooperating with von Hippel Lindau to regulate tubulin stability. [FBrf0218047] Tokusumi et al., 2012, PLoS ONE 7(7): e41604 Gene regulatory networks controlling hematopoietic progenitor niche cell production and differentiation in the Drosophila lymph gland. [FBrf0219204] Cachero et al., 2011, PLoS Biol. 9(1): e1000568 The gene regulatory cascade linking proneural specification with differentiation in Drosophila sensory neurons. [FBrf0212891] Friedman et al., 2011, Sci. Signal. 4(196): rs10 Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. [FBrf0216513] Meyer et al., 2011, Curr. Biol. 21(6): 485--491 Interkinetic nuclear migration is a broadly conserved feature of cell division in pseudostratified epithelia. [FBrf0213254] Sharma et al., 2011, Chemosphere 82(3): 370--376 Transcriptome analysis provides insights for understanding the adverse effects of endosulfan in Drosophila melanogaster. [FBrf0212610] Siudeja et al., 2011, EMBO Mol. Med. 3(12): 755--766 Impaired Coenzyme A metabolism affects histone and tubulin acetylation in Drosophila and human cell models of pantothenate kinase associated neurodegeneration. [FBrf0216869] Weake et al., 2011, Genes Dev. 25(14): 1499--1509 Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation. [FBrf0214372] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...