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General Information
Symbol
Dmel\tup
Species
D. melanogaster
Name
tailup
Annotation Symbol
CG10619
Feature Type
FlyBase ID
FBgn0003896
Gene Model Status
Stock Availability
Gene Summary
tailup (tup) encodes a transcription factor that regulates neuronal sub-type identity, including motor, serotonergic and dopaminergic neuron identity. It regulates germ band retraction, dorsal closure, muscle and heart development. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
Also Known As

islet, isl

Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:18,859,507..18,881,261 [-]
Recombination map
2-54
RefSeq locus
NT_033779 REGION:18859507..18881261
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (27 terms)
Molecular Function (6 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001197649
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001197649
(assigned by GO_Central )
Biological Process (20 terms)
Terms Based on Experimental Evidence (16 terms)
CV Term
Evidence
References
inferred from mutant phenotype
involved_in axon guidance
inferred from mutant phenotype
involved_in chaeta development
inferred from mutant phenotype
inferred from mutant phenotype
involved_in dendrite guidance
inferred from mutant phenotype
involved_in dorsal closure
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
involved_in heart development
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Chi; FB:FBgn0013764
inferred from direct assay
inferred from mutant phenotype
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
involved_in axonogenesis
inferred from biological aspect of ancestor with PANTHER:PTN001197637
(assigned by GO_Central )
involved_in head involution
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN001197637
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000653991
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001197649
(assigned by GO_Central )
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN001197649
(assigned by GO_Central )
Protein Family (UniProt)
-
Summaries
Gene Snapshot
tailup (tup) encodes a transcription factor that regulates neuronal sub-type identity, including motor, serotonergic and dopaminergic neuron identity. It regulates germ band retraction, dorsal closure, muscle and heart development. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
LIM HOMEOBOX TRANSCRIPTION FACTORS -
LIM homeobox transcription factors are sequence-specific DNA binding proteins that regulate transcription. They have two N-terminal LIM domains (cysteine-rich, double-zinc finger motifs) and a centrally located sequence-specific DNA binding homeodomain. (Adapted from PMID:24676471 and FBrf0125444).
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
tup: tailup
Embryonic lethal. Head broad. Germband shortening apparently ceases early, resulting in the posteriormost three segments remaining on dorsal side of the embryo.
Summary (Interactive Fly)

transcription factor - homeodomain and LIM domain - required in combination with other transcription factors for serotonergic and dopaminergic neuron identity

Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\tup for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0081111
3251
534
FBtr0081112
3314
465
FBtr0342921
2270
534
Additional Transcript Data and Comments
Reported size (kB)

3.237 (longest cDNA)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0080661
58.0
534
7.85
FBpp0080662
51.8
465
7.63
FBpp0309708
58.0
534
7.85
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

534 aa isoforms: tup-PA, tup-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\tup using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

antennal anlage

Comment: reported as procephalic ectoderm anlage

central brain anlage

Comment: reported as procephalic ectoderm anlage

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

visual anlage

Comment: reported as procephalic ectoderm anlage

Additional Descriptive Data

tup transcripts are detected in the dorsal ectoderm starting in embryonic stage 10. tup transcripts are also present in Mef2-positive myocardial cells.

tup transcript is first detected in stage 10 embryos in the precursors of the dorsal vessel, pharynx, and amnioserosa. By stage 16 expression has narrowed to the dorsal vessel and the alary and pharyngeal muscles. tup CNS expression is detected in stage 12 embryos in subsets of cells in the ventral nerve cord and brain. Expression is not found in neuroblasts, but in neuronal progeny. By stage 13, there are 20 cells expressing tup per hemisegment, and this pattern is retained through embryonic development. Reporter construct expression (Btau\MAPTisl.H.T:Hsap\MYC) indicates that the tup expressing cells are a subset of motor neurons and interneurons.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

In embryos, tup can be detected in the cardiac cells of the heart tip, but not in the most anterior cardioblasts, including the pair of anterior cardioblasts.

In early embryonic stage 11, immediately after MP3 division, tup is absent from the H-cell and H-cell sib. Later in stage 11, tup is present in both the H-cell and H-cell sib. By the end of stage 11, tup is present in the H-cell but absent in H-cell sib. Expression in the H-cell remains on through the end of embryogenesis.

tup protein is detected in a broad domain along the dorsal side of the embryo within the ectodermal layer starting in embryonic stage 10. Expression in the cardiogenic mesoderm begins at mid-stage 11 in ~10 small clusters of cells that are also positive for eve. The tup-expressing cells are part of the pericardial lineage. By late stage 11, tup protein expression expands within the cardiogenic mesoderm (is coexpressed with tin throughout the cardogenic mesoderm) and continues in all myocardial cells during embryogenesis. tup is also expressed in at least two of the tin-positive pericardial cells and all odd-expressing pericardial cells in each hemisegment. In lymph glands, tup is only observed in some of the odd-positive cells. tup is also observed in the amnioserosa.

Approximately 50% of adPNs and 80% of lPNs express tup.

tup expression is confined to the most proximal region of the wing disc, which correspons to part of the prospective notum, as the early late first/early second instar larval stage. During second instar and part of third instar, tup is expressed in the entire medial notum area and extends into the lateral notum. In mid-late third instar, strong expression is maintained in the posterior medial and part of the lateral notum. The most lateral region of the posterior notum is free of tup expression.

The pattern of tup protein expression closely matches that of tup transcript expression. CNS expression is detected in subsets of cells in the brain and ventral nerve cord starting at stage 12; the pattern of about 20 tup expressing cells per hemisegment seen by stage 13 is retained through the rest of embryogenesis.

Marker for
Subcellular Localization
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
Expression Deduced from Reporters
Reporter: P{GawB}tupJQ3
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{islH-tau-myc}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{tup-EGFP.F4}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{tup-GAL4.AME-R}
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\tup in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
EMBL-EBI Single Cell Expression Atlas
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 14 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of tup
Transgenic constructs containing regulatory region of tup
Aberrations (Deficiencies and Duplications) ( 11 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
11 of 15
No
Yes
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
10 of 15
No
Yes
Rattus norvegicus (Norway rat) (3)
13 of 13
Yes
Yes
8 of 13
No
Yes
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (2)
5 of 12
Yes
Yes
5 of 12
Yes
Yes
Danio rerio (Zebrafish) (4)
13 of 15
Yes
Yes
10 of 15
No
Yes
9 of 15
No
Yes
5 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
12 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (4)
1 of 9
Yes
No
1 of 9
Yes
No
1 of 9
Yes
Yes
1 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091909O3 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915070W )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W09CS )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0998 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G09L9 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    suppressible
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-54
    Cytogenetic map
    Sequence location
    2L:18,859,507..18,881,261 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    37B1-37B1
    Limits computationally determined from genome sequence between P{EP}CG10413EP2164 and P{lacW}l(2)37Dbk16106&P{lacW}Catsupk05424
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    37A-37A
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (20)
    Genomic Clones (33)
    cDNA Clones (104)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       

      monoclonal

      Commercially Available Antibodies
       
      Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of
      Additional comments
      Other Comments

      tup is required to specify DA2 muscle identity via repression of kn transcription.

      tup is required for the specification of the notum territory.

      eve and tup constitute a bimodal switch regulating axonal growth and directing motor axons to ventral (tup) or dorsal (eve) regions of the muscle field during embryogenesis.

      Isolated from an embryonic expression library, using monoclonal antibodies to rat Islet-1 and Islet-2.

      tup has been cloned and characterised.

      A member of the ush-group of genes which are required for maintenance of the amnioserosa once it has differentiated.

      Mutants affect amnioserosa differentiation as well as germ band retraction.

      Mutations in zygotic gene tup do not interact with RpII140wimp.

      Zygotically active locus involved in the terminal developmental program in the embryo.

      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 47 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      EMBL-EBI Single Cell Expression Atlas
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      MARRVEL_MODEL
      modMine - A data warehouse for the modENCODE project
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (18)
      Reported As
      Symbol Synonym
      l(2)37Aa
      l(2)E41
      Name Synonyms
      Secondary FlyBase IDs
      • FBgn0002017
      • FBgn0015395
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (225)