FlyBase Gene Report: Dmel\RpS27A

General Information

Symbol

Dmel\RpS27A

Species

D. melanogaster

Name

Ribosomal protein S27A

Annotation Symbol

CG5271

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003942

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Snapshot

Contributions welcome

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

l(2)04820, DUb80, mfs48, Ubiquitin, Ubi-m

Key Links

Genomic Location

Cytogenetic map

31E1-31E1

Sequence location

2L:10,408,352..10,410,456 [+]

Recombination map

2-42

RefSeq locus

NT_033779 REGION:10408352..10410456

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables protein tag

inferred from sequence or structural similarity with SGD:S000003962

(FlyBase Curators, 2008--)

inferred from biological aspect of ancestor with PANTHER:PTN000071508

(assigned by GO_Central )

(Gaudet et al., 2011)

enables structural constituent of ribosome

inferred from electronic annotation with InterPro:IPR002906

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

traceable author statement

(Marygold et al., 2007)

enables ubiquitin protein ligase binding

inferred from biological aspect of ancestor with PANTHER:PTN000071508

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in cytoplasmic translation

traceable author statement

(Marygold et al., 2007)

involved_in modification-dependent protein catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000071508

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in protein ubiquitination

inferred from biological aspect of ancestor with PANTHER:PTN000071508

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with SGD:S000003962

(FlyBase Curators, 2008--)

involved_in translation

inferred from electronic annotation with InterPro:IPR002906, InterPro:IPR011332

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000071508

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of cytosolic small ribosomal subunit

inferred from biological aspect of ancestor with PANTHER:PTN002241455

(assigned by GO_Central )

(Gaudet et al., 2011)

traceable author statement

(Marygold et al., 2007)

located_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000071508

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

CYTOPLASMIC SMALL RIBOSOMAL PROTEINS

UBIQUITIN

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

CYTOPLASMIC SMALL RIBOSOMAL PROTEINS -: Cytoplasmic ribosomes are found on the endoplasmic reticulum and in the cytoplasm. They translate all mRNAs produced from nuclear genes and perform the majority of cellular protein synthesis. Ribosomes are formed by two ribonucleoprotein subunits, large and small. The principle function of the small subunit is to bind mRNA. (Adapted from FBrf0205398)
UBIQUITIN -: Ubiquitin is a small (76 amino acid), highly conserved protein found in all eukaryotes. Through its covalent attachment to other proteins, ubiquitin can modify protein activity in numerous ways. The most well known is the targeting of proteins for proteasomal degradation. Ubiquitin is often expressed as a fusion protein, either as polyubiquitin or fused with the ribosomal proteins L40 and S27a. Ubiquitin monomers are generated by post-translational cleavage. (Adapted from PMID:12729753 and PMID:27012465)

Protein Function (UniProtKB)

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-48-linked is involved in protein degradation via the proteasome. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity). Ribosomal protein S27a is a component of the 40S subunit of the ribosome.

(UniProt, P15357)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

mfs(2)31: male-female-sterile (2) in region 31

Homozygotes have short thin bristles; relative viability 25% at 25; hemizygotes lethal. Both males and females sterile at 25 but some fertility observed in flies raised at 23. Males raised at 28.5 have no motile sperm and some spermatids have micronuclei, and occasionally two basal bodies and axonemes are observed.

Ubi-m: Ubiquitin-monomeric

Structural gene for ubiquitin (Lee et al., 1988). Ubi-m codes for a fusion protein whose single ubiquitin sequence is attached to a tail polypeptide with 65% identity to the yeast ubiquitin tail protein and 82% identity to the human ubiquitin tail protein. Ubiquitin-monomeric is not inducible by heat shock.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\RpS27A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Shares 5' UTR with upstream gene.

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

RpS27A-RA

FBtr0080016

NM_057430

592

156

Additional Transcript Data and Comments

Reported size (kB)

0.9 (northern blot)

(Barrio et al., 1994, Lee et al., 1988)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

RpS27A-PA

FBpp0079606

17.9

156

10.44

NP_476778

AAF52941

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

156 (aa)

(Lee et al., 1988)

Comments

External Data

Subunit Structure (UniProtKB)

Ribosomal protein S27a is part of the 40S ribosomal subunit.

(UniProt, P15357)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P15357

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpS27A using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male & spermatogenesis

germinal proliferation center

(Lu et al., 2013)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval fat body

(Barrio et al., 1994)

embryonic/larval salivary gland

(Barrio et al., 1994)

imaginal disc

(Barrio et al., 1994)

Malpighian tubule

(Barrio et al., 1994)

adult stage

ovary

(Barrio et al., 1994)

embryonic stage -- adult stage

(Barrio et al., 1994)

Additional Descriptive Data

RpS27A transcript is expressed at the apical end of the testis.

(Lu et al., 2013)

RpS27A transcripts are detected in all stages and in all tissues tested. Tissues include larval third instar Malpighian tubules, imaginal discs, fat body, and salivary gland and adult ovary.

(Barrio et al., 1994)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\RpS27A in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\RpS27A

View exonic expression by tissue for Dmel\RpS27A

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0003942

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5271-RA

Flygut - An atlas of the Drosophila adult midgut

FBgn0003942

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of RpS27A

Transgenic constructs containing regulatory region of RpS27A

Deletions and Duplications ( 19 )

Disrupted in

Df(2L)BSC144

(Marygold et al., 2007)

Df(2L)BSC209

(Bloomington Drosophila Stock Center, 2014.8.18, Marygold et al., 2007, Christensen and Cook, 2006.8.30)

Df(2L)BSC342

(Christensen and Cook, 2007.10.29)

Df(2L)BSC453

(Christensen et al., 2008.4.15)

Df(2L)ED13205

(Marygold et al., 2007)

Df(2L)G2

(Clegg et al., 1993)

Df(2L)J2

(Spradling et al., 1999, BDGP Project Members, 1994-1999, Clegg et al., 1993, Sinclair et al., 1992)

Df(2L)J3

(Clegg et al., 1993)

Df(2L)J4

(Marygold et al., 2007)

Df(2L)J16

(Clegg et al., 1993)

Df(2L)J27

(Clegg et al., 1993, Sinclair et al., 1992)

Df(2L)J39

(Marygold et al., 2007, Clegg et al., 1993, Sinclair et al., 1992)

Df(2L)J77

(Mottus et al., 1997, Clegg et al., 1993, Salas and Lengyel, 1984)

Df(2L)J106

(Marygold et al., 2007, Clegg et al., 1993, Sinclair et al., 1992, Salas and Lengyel, 1984)

Df(2L)J233

(Sinclair et al., 1992, Salas and Lengyel, 1984)

In(2L)EIN52^LEIN53^R

(Marygold et al., 2007)

Not disrupted in

Df(2L)J1

(Clegg et al., 1993)

Df(2L)J17

(Clegg et al., 1993)

T(Y;2)J^rv99

(Salas and Lengyel, 1984)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

RpS27A^GD10725

lethal | recessive

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

RpS27A^GD10725

partially lethal

RpS27A^mfs31

Sterility

Allele

female sterile | heat sensitive

RpS27A^mfs31

male sterile | heat sensitive

RpS27A^mfs31

Phenotype manifest in

Allele

macrochaeta

RpS27A^mfs31

spermatid

RpS27A^mfs31

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (21)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\RPS27A

12 of 15

Yes

3 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (34)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Rps27a

7 of 15

Yes

Mmus\LOC100042019

6 of 15

Yes

3 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Gm8797

1 of 15

Yes

Mmus\Gm9843

1 of 15

Mmus\Isg15

1 of 15

Mmus\Oas1a

1 of 15

Yes

Mmus\Oas1c

1 of 15

Yes

Mmus\Oas1d

1 of 15

Yes

Mmus\Oas1e

1 of 15

Yes

Mmus\Oas1f

1 of 15

Yes

Mmus\Oas1g

1 of 15

Yes

Mmus\Oas1h

1 of 15

Yes

Mmus\Oasl1

1 of 15

Yes

Mmus\Oasl2

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Rattus norvegicus (Norway rat) (36)

Rnor\Rps27a

11 of 13

Yes

Rnor\Nedd8

3 of 13

Rnor\LOC679594

2 of 13

Yes

Rnor\LOC100360645

2 of 13

Yes

Rnor\LOC100363494

2 of 13

Yes

Rnor\LOC100910768

2 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Rnor\Oas1a

1 of 13

Yes

Rnor\Oas1b

1 of 13

Yes

Rnor\Oas1d

1 of 13

Yes

Rnor\Oas1e

1 of 13

Yes

Rnor\Oas1g

1 of 13

Yes

Rnor\Oas1i

1 of 13

Yes

Rnor\Oasl2

1 of 13

Yes

Rnor\Oasl

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Rnor\Rps27a-ps5

1 of 13

Yes

Rnor\Rps27a-ps6

1 of 13

Yes

Rnor\Ubb

1 of 13

Rnor\Ubc

1 of 13

Rnor\Ubd

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (13)

Xtro\rps27a

7 of 12

Yes

3 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (19)

Drer\rps27a

11 of 15

Yes

3 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\rad23aa

1 of 15

Drer\rad23ab

1 of 15

Drer\rad23b

1 of 15

Drer\si:ch211-202a12.4

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (16)

Cele\ubl-1

9 of 15

Yes

Cele\F34H10.1

3 of 15

Yes

Cele\K08C9.7

3 of 15

Yes

Cele\ned-8

3 of 15

Cele\F52C6.2

2 of 15

Yes

Cele\ubq-2

2 of 15

Cele\C16C8.4

1 of 15

Yes

Cele\C16C8.5

1 of 15

Yes

Cele\C16C8.11

1 of 15

Yes

Cele\C16C8.13

1 of 15

Yes

Cele\C16C8.14

1 of 15

Yes

Cele\F14D2.11

1 of 15

Yes

Cele\F52C6.3

1 of 15

Cele\F52C6.4

1 of 15

Yes

Cele\ubq-1

1 of 15

Cele\ubql-1

1 of 15

Arabidopsis thaliana (thale-cress) (15)

Atha\AT1G23410

7 of 9

Yes

Atha\UBQ5

7 of 9

Yes

Atha\UBQ6

7 of 9

Yes

Atha\AT1G53930

1 of 9

Atha\AT1G53950

1 of 9

Yes

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

Yes

Atha\UBQ10

1 of 9

Atha\UBQ12

1 of 9

Atha\UBQ13

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\RPS31

11 of 15

Yes

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\SPAC589.10c

9 of 12

Yes

Spom\ubi3

9 of 12

Yes

Spom\ned8

1 of 12

Spom\ubi4

1 of 12

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190IOU )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA24218

Drosophila persimilis

Dper\GL18966

Drosophila willistoni

Dwil\GK18589

Drosophila virilis

Dvir\GJ21841

Drosophila mojavensis

Dmoj\GI10618

Drosophila grimshawi

Dgri\GH13542

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500RT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17022142

Musca domestica

House fly

Mdoa\17025753

Musca domestica

House fly

Mdoa\17011646

Musca domestica

House fly

Mdoa\17014836

Musca domestica

House fly

Mdoa\17017692

Glossina morsitans

Tsetse fly

Gmor\GMOY007966

Glossina morsitans

Tsetse fly

Gmor\GMOY005690

Mayetiola destructor

Hessian fly

Mdes\Mdes004661

Mayetiola destructor

Hessian fly

Mdes\Mdes009910

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL007168

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL000679

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL003888

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013536

Aedes aegypti

Yellow fever mosquito

Aaeg\Ub52

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013680

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL000795

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL008920

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL002368

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL003881

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL003877

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL011219

Anopheles darlingi

American malaria mosquito

Adar\ADAC006535

Anopheles darlingi

American malaria mosquito

Adar\ADAC006534

Anopheles darlingi

American malaria mosquito

Adar\ADAC006215

Anopheles darlingi

American malaria mosquito

Adar\ADAC009123

Anopheles gambiae

Malaria mosquito

Agam\AGAP002599

Anopheles gambiae

Malaria mosquito

Agam\AGAP001969

Anopheles gambiae

Malaria mosquito

Agam\AGAP007913

Anopheles gambiae

Malaria mosquito

Agam\AGAP013742

Anopheles gambiae

Malaria mosquito

Agam\AGAP001971

Anopheles gambiae

Malaria mosquito

Agam\Rpl40

Anopheles gambiae

Malaria mosquito

Agam\AGAP001970

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ013710

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ015770

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ013324

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003598

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003589

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002413

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003099

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ001487

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003588

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00LT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA001415

Bombyx mori

Silkmoth

Bmor\BGIBMGA001549

Bombyx mori

Silkmoth

Bmor\BGIBMGA011273

Bombyx mori

Silkmoth

Bmor\UBI1

Bombyx mori

Silkmoth

Bmor\BGIBMGA007334

Danaus plexippus

Monarch butterfly

Dple\DPOGS208502-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS204814-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS202511-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS211179-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL012420

Heliconius melpomene

Postman butterfly

Hmel\HMEL011485

Heliconius melpomene

Postman butterfly

Hmel\HMEL007444

Heliconius melpomene

Postman butterfly

Hmel\HMEL008746

Heliconius melpomene

Postman butterfly

Hmel\HMEL016506

Heliconius melpomene

Postman butterfly

Hmel\HMEL013556

Apis florea

Little honeybee

Aflo\2965419524

Apis florea

Little honeybee

Aflo\5081404820

Apis florea

Little honeybee

Aflo\2823877220

Apis florea

Little honeybee

Aflo\2147486065

Apis florea

Little honeybee

Aflo\2147485985

Apis florea

Little honeybee

Aflo\2147696803

Apis florea

Little honeybee

Aflo\3378550612

Apis mellifera

Western honey bee

Amel\RpL40

Apis mellifera

Western honey bee

Amel\LOC409675

Apis mellifera

Western honey bee

Amel\GB53968

Apis mellifera

Western honey bee

Amel\GB45661

Apis mellifera

Western honey bee

Amel\RpS27A

Apis mellifera

Western honey bee

Amel\GB53967

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP18939

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP10609

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP25239

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP11826

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP16856

Bombus terrestris

Buff-tailed bumblebee

Bter\15201502

Bombus terrestris

Buff-tailed bumblebee

Bter\15203048

Bombus terrestris

Buff-tailed bumblebee

Bter\15204858

Bombus terrestris

Buff-tailed bumblebee

Bter\15216577

Bombus terrestris

Buff-tailed bumblebee

Bter\15205336

Linepithema humile

Argentine ant

Lhum\LH16507

Linepithema humile

Argentine ant

Lhum\LH26187

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0021af

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002657

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0025f3

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0020f3

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG023157

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG011275

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG006666

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG017061

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG013235

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG031153

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_07754

Tribolium castaneum

Red flour beetle

Tcas\TC014299

Tribolium castaneum

Red flour beetle

Tcas\TC009978

Tribolium castaneum

Red flour beetle

Tcas\TC032827

Pediculus humanus

Human body louse

Phum\PHUM528640

Pediculus humanus

Human body louse

Phum\PHUM442590

Pediculus humanus

Human body louse

Phum\PHUM602270

Rhodnius prolixus

Kissing bug

Rpro\RPRC006442

Rhodnius prolixus

Kissing bug

Rpro\RPRC008770

Rhodnius prolixus

Kissing bug

Rpro\RPRC012247

Rhodnius prolixus

Kissing bug

Rpro\RPRC014620

Cimex lectularius

Bed bug

Clec\CLEC008644

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI086543

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI064888

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI066032

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005197

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000086

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007765

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003315

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000076

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008479

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_08320

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_09025

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_08554

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_02553

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_15362

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X08AW )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR005736

Strigamia maritima

European centipede

Smar\SMAR005739

Strigamia maritima

European centipede

Smar\SMAR005737

Ixodes scapularis

Black-legged tick

Isca\ISCW010407

Ixodes scapularis

Black-legged tick

Isca\ISCW023133

Ixodes scapularis

Black-legged tick

Isca\ISCW004394

Ixodes scapularis

Black-legged tick

Isca\ISCW000125

Stegodyphus mimosarum

African social velvet spider

Smim\KFM70679.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM70936.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur06g00900

Tetranychus urticae

Two-spotted spider mite

Turt\tetur17g01490

Tetranychus urticae

Two-spotted spider mite

Turt\tetur03g06910

Tetranychus urticae

Two-spotted spider mite

Turt\tetur18g03450

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_52566

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_210344

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_243700

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_17671

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_48503

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_9558

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_302125

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_48454

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G178I )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Rps27a

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Nedd8_2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ubq/RL40fp

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-UbcL

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-C19orf50L

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Polyq

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000020815

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000005834

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000002990

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000018890

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000005008

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000007605

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000004509

Gallus gallus

Domestic chicken

Ggal\RPS27A

Gallus gallus

Domestic chicken

Ggal\C19orf50

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (11)

Gene Symbol

Score

Source

Alignment

5 of 10

4 of 10

4 of 10

3 of 10

3 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RPS27A; ribosomal protein S27a

12 of 15

191343

NEDD8; NEDD8 ubiquitin like modifier

3 of 15

603171

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

RpS27A - Nrd1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Yoon et al., 2017)

RpS27A - Rack1

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS27A - Raf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

RpS27A - RpL10

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL10Ab

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL13A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL14

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL18A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL19

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL22

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL23A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL28

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL30

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL31

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL32

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL34a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL35

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS27A - RpL35A

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS27A - RpL36

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL36A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL37A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL37a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL38

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL39

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS27A - RpL4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL40

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS27A - RpL41

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS27A - RpL5

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL7A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpL9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpLP0

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpLP1

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpLP2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS10a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS14a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS14b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS15Aa

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS16

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS19a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS20

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS25

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS28b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS30

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS27A - RpS3A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS5a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - RpS9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - bbx

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

RpS27A - eEF2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS27A - her

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

RpS27A - slim

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

RpS27A - sta

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS27A - vig2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RNA-protein

Physical Interaction

Assay

References

RpS27A - 18SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS27A - 28SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS27A - 2SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS27A - 5.8SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS27A - 5SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Ribosomal protein S27a is part of the 40S ribosomal subunit.

(UniProt, P15357 )

Linkouts

BioGRID - A database of protein and genetic interactions.

60502

DroID - A comprehensive database of gene and protein interactions.

FBgn0003942

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34420

MIST (protein-protein) - An integrated Molecular Interaction Database

RpS27A

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ribosome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-42

Cytogenetic map

31E1-31E1

Sequence location

2L:10,408,352..10,410,456 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

31E1-31E1

Limits computationally determined from genome sequence between P{lacW}RnrL^k06709 and P{lacW}KdelR^k00311

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

31E-31E

(Barrio, 1992.11.5)

31E3-31E5

(determined by in situ hybridisation)

(Spradling et al., 1999)

31E-31E

(determined by in situ hybridisation)

(Ranz et al., 1997, Izquierdo, 1994)

31E3-31E5

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-42

(FlyBase, 2016)

2-35.5

(Comeron et al., 2012)

(Barrio, 1992.11.5)

2-41.35

Left of (cM)

Right of (cM)

Notes

Based on 23 recombinants between J and RpS27A with da as a middle marker.

Stocks and Reagents

Stocks (10)

Bloomington

2531

RpS27A^mfs31/CyO

11387

P{PZ}RpS27A⁰⁴⁸²⁰ cn¹/CyO; ry⁵⁰⁶

85035

w¹¹¹⁸; PBac{PB}Ip259^c02960 RpS27A^c02960/CyO

35418

y¹ sc^* v¹ sev²¹; P{TRiP.GL00340}attP2

78986

y¹ w^*; TI{CRIMIC.TG4.0}RpS27A^{CR00689-TG4.0}/SM6a

FlyORF

F001616

M{UAS-RpS27A.ORF}ZH-86Fb

F000847

M{UAS-RpS27A.ORF.3xHA}ZH-86Fb

107386

v105501

v34325

w¹¹¹⁸; P{GD10725}v34325/CyO

Genomic Clones (28)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (338)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003942

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003942

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34420

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: RpS27A l(2)04820

(Spradling et al., 1999)

Additional comments

"RpS27A" likely corresponds to "M(2)31E".

(Marygold et al., 2007)

One or more of the processed transcripts for this gene share(s) untranslated sequences with a transcript of an adjacent gene, but encode(s) a single open reading frame (ORF). The non-overlapping ORFs that share untranslated sequences are represented by RpS27A and Ip259.

(FlyBase Genome Annotators, 2002-2003)

Other Comments

Likely Minute gene.

(Marygold et al., 2007)

Deletions removing RpS27A but no other cytoplasmic ribosomal protein-encoding genes show Minute phenotypes.

(Marygold et al., 2007)

RpS27A and Ip259 share a 5' untranslated exon, however, alternative splicing produces two structurally unrelated proteins which do not share any common sequences.

(Mottus et al., 1997)

The genomic organisation of the RpS27A locus has been characterised.

(Barrio et al., 1994)

Antibody staining of polytene chromosomes shows that ubiquitin is mainly associated with the compact and stabilized structure that forms the bands rather than with the more decondensed and destabilized protein-DNA structure that forms interbands and puffs.

(Izquierdo, 1994)

Four (unnamed) recessive lethal alleles have been isolated during a cytogenetic analysis of chromosomal region 31.

(Clegg et al., 1993)

RpS27A expression has been studied in heat shocked aging flies.

(Niedzwiecki and Fleming, 1993)

Recessive alleles suppress position-effect variegation of In(1)w^m4 when homozygous. The suppressor phenotypes are sexually dimorphic.

(Sinclair et al., 1992)

Structural gene for ubiquitin (Lee et al., 1988). Ubi-f80 codes for a fusion protein whose single ubiquitin sequence is attached to a tail polypeptide with 65% identity to the yeast ubiquitin tail protein and 82% identity to the human ubiquitin tail protein. Ubiquitin-monomeric is not inducible by heat shock.

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 144 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34420

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P15357

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003942

Flygut - An atlas of the Drosophila adult midgut

FBgn0003942

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

104239

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34420

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG5271

modMine - A data warehouse for the modENCODE project

FBgn0003942

Linkouts

BioGRID - A database of protein and genetic interactions.

60502

DroID - A comprehensive database of gene and protein interactions.

FBgn0003942

DRSC - Results frm RNAi screens

FBgn0003942

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0003942

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5271-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003942

FlyMine - An integrated database for Drosophila genomics

FBgn0003942

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34420

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ribosome

MIST (protein-protein) - An integrated Molecular Interaction Database

RpS27A

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

CG5271

(Kolkhof et al., 2017, Neuert et al., 2017, Caizzi et al., 2016, Ristic et al., 2016, Lu et al., 2014, Sing et al., 2014, Schertel et al., 2013, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Hughes et al., 2008, Goldman and Arbeitman, 2007, Marygold et al., 2007, Quinones-Coello, 2007)

DUb80

(Mottus et al., 1997, Barrio et al., 1994, Barrio, 1992.11.5)

Dub80

RpS27

(Lu et al., 2013)

UB3-D

(Niedzwiecki and Fleming, 1993)

Ubi-f80

Ubi-m

(Lindsley and Zimm, 1992)

Ubiq

(Wesley and Mok, 2003)

l(2)04820

(Mesilaty-Gross et al., 1999, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

l(2)31Ei

mfs(2)31

mfs(2)48

mfs48

(Cummings and Cronmiller, 1994, Salas and Lengyel, 1984)

Name Synonyms

CG5271

(Goldman and Arbeitman, 2007)

Ribosomal protein S27A

Ubiquitin

(Herz et al., 2006, Wesley and Mok, 2003)

Ubiquitin fusion 80

Ubiquitin-monomeric

(Lindsley and Zimm, 1992)

male female sterile 31

ubiquitin

(Murphey and Godenschwege, 2002)

Secondary FlyBase IDs

FBgn0002728
FBgn0010585
FBgn0064114

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (96)

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