FlyBase Gene Report: Dmel\Ubi-p63E

General Information

Symbol

Dmel\Ubi-p63E

Species

D. melanogaster

Name

Ubiquitin-63E

Annotation Symbol

CG11624

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003943

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Snapshot

Ubiquitin-63E (Ubi-p63E) encodes a polyubiquitin precursor: once processed by deubiquitinating enzymes, it produces ten ubiquitin molecules, which are predominantly conjugated to target proteins. Ubiquitin conjugation is involved in a wide range of biological processes, such as protein degradation, endocytosis of plasma membrane proteins, protein trafficking, DNA-damage response and inflammation and immune system response. [Date last reviewed: 2018-09-06]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

Ub, ubiquitin, Ubi-p, polyubiquitin, ubi63E

Key Links

Genomic Location

Cytogenetic map

63F5-63F5

Sequence location

3L:3,899,259..3,903,184 [-]

Recombination map

3-9

RefSeq locus

NT_037436 REGION:3899259..3903184

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

protein tag

inferred from biological aspect of ancestor with PANTHER:PTN000071508

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with SGD:S000003962

(FlyBase Curators, 2008--)

ubiquitin protein ligase binding

inferred from biological aspect of ancestor with PANTHER:PTN000071508

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

autophagy

inferred from mutant phenotype

(Chang et al., 2013)

larval midgut cell programmed cell death

inferred from mutant phenotype

(Chang et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

modification-dependent protein catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000071508

(assigned by GO_Central )

(Gaudet et al., 2011)

protein ubiquitination

inferred from biological aspect of ancestor with PANTHER:PTN000071508

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with SGD:S000003962

(FlyBase Curators, 2008--)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000071508

(assigned by GO_Central )

(Gaudet et al., 2011)

nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000071508

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

UBIQUITIN

Protein Family (UniProt)

Belongs to the ubiquitin family. (P0CG69)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

UBIQUITIN -: Ubiquitin is a small (76 amino acid), highly conserved protein found in all eukaryotes. Through its covalent attachment to other proteins, ubiquitin can modify protein activity in numerous ways. The most well known is the targeting of proteins for proteasomal degradation. Ubiquitin is often expressed as a fusion protein, either as polyubiquitin or fused with the ribosomal proteins L40 and S27a. Ubiquitin monomers are generated by post-translational cleavage. (Adapted from PMID:12729753 and PMID:27012465)

Protein Function (UniProtKB)

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-48-linked is involved in protein degradation via the proteasome. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity).

(UniProt, P0CG69)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Ubi-p: Ubiquitin-polymeric

Structural gene for ubiquitin (Arribas et al., 1988); structure and expression described by Lee et al. (1988). Threefold increase in level of gene by heat shock. An Ubi-p - lacZ fusion gene introduced by germ line transformation has been expressed at high levels at all life stages tested (embryonic, larval, pupal, and adult).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ubi-p63E for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Apparent introns not annotated: probable artifacts due to repetitive sequence.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ubi-p63E-RA

FBtr0073177

NM_168043

2521

763

Ubi-p63E-RB

FBtr0073178

NM_079185

2759

763

Ubi-p63E-RC

FBtr0073179

NM_206272

2710

763

Ubi-p63E-RD

FBtr0333142

NM_001274454

2624

763

Additional Transcript Data and Comments

Reported size (kB)

4.4 (northern blot)

(Lee et al., 1988)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Ubi-p63E-PA

FBpp0073034

85.8

763

7.95

NP_728908

AAF47806

Ubi-p63E-PB

FBpp0073035

85.8

763

7.95

NP_523909

AAG22241

Ubi-p63E-PC

FBpp0089269

85.8

763

7.95

NP_995994

AAS64964

Ubi-p63E-PD

FBpp0305348

85.8

763

7.95

NP_001261383

AGB94078

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

763 aa isoforms: Ubi-p63E-PA, Ubi-p63E-PB, Ubi-p63E-PC, Ubi-p63E-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

231 (aa)

(Lee et al., 1988)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ubi-p63E using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

spermatogenesis & adult stage | male

spermatocyte

(Lu et al., 2013)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage & larval stage & pupal stage

(Lee et al., 1988)

embryonic stage -- adult stage

(Lee et al., 1988)

Additional Descriptive Data

Ubi-p63E transcript is expressed in spermatocytes. Expression is isoform-specific; very little Ubi-p63E-RC transcript is detected in spermatocytes, while Ubi-p63E-RA has higher expression levels than other isoforms.

(Lu et al., 2013)

Ubi-p63E transcripts are observed in all stages assayed. A reporter construct was used to show that they are expressed in a tissue-general pattern in all life stages assayed.

(Lee et al., 1988)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ubi-p63E in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ubi-p63E

View exonic expression by tissue for Dmel\Ubi-p63E

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0003943

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG11624-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11624

Flygut - An atlas of the Drosophila adult midgut

FBgn0003943

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 11 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ubi-p63E

Transgenic constructs containing regulatory region of Ubi-p63E

Deletions and Duplications ( 4 )

Disrupted in

Df(3L)Exel6098

(Lu et al., 2013, Ryder, 2004.4.26)

Not disrupted in

Df(3L)ED208

(Lu et al., 2013)

Df(3L)ED4341

(Lu et al., 2013)

Partially disrupted in

Df(3L)Ubi-p63E^12c

(Lu et al., 2013)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable (with Ubi-p63E^12c)

Ubi-p63E^23b

viable (with Ubi-p63E^23b)

Ubi-p63E^12c

Sterility

Allele

female fertile

Ubi-p63E^12c

female fertile (with Ubi-p63E^12c)

Ubi-p63E^23b

female fertile (with Ubi-p63E^23b)

Ubi-p63E^12c

fertile

Ubi-p63E^EY07341

male sterile

male sterile (with Ubi-p63E^12c)

Ubi-p63E^23b

male sterile (with Ubi-p63E^23b)

Ubi-p63E^12c

Other Phenotypes

Allele

meiotic cell cycle defective | cell autonomous | male limited | somatic clone

Ubi-p63E^12c

meiotic cell cycle defective | male limited

meiotic cell cycle defective | male limited (with Df(3L)Exel6098)

Ubi-p63E^EY07341

meiotic cell cycle defective | male limited (with Ubi-p63E^12c)

meiotic cell cycle defective | male limited (with Ubi-p63E^23b)

meiotic cell cycle defective | male limited (with Ubi-p63E^EY07341)

meiotic cell cycle defective | male limited (with Ubi-p63E^Z3-2534)

meiotic cell cycle defective | male limited (with Ubi-p63E^Z3-3969)

meiotic cell cycle defective | male limited (with Ubi-p63E^Z3-4216)

meiotic cell cycle defective | male limited (with Ubi-p63E^Z3-5802)

Phenotype manifest in

Allele

spermatocyte

Ubi-p63E^unspecified

spermatocyte | cell autonomous | somatic clone

Ubi-p63E^12c

testis

Ubi-p63E^12c

testis (with Df(3L)Exel6098)

Ubi-p63E^EY07341

testis (with Ubi-p63E^12c)

testis (with Ubi-p63E^23b)

testis (with Ubi-p63E^EY07341)

testis (with Ubi-p63E^Z3-2534)

testis (with Ubi-p63E^Z3-3969)

testis (with Ubi-p63E^Z3-4216)

testis (with Ubi-p63E^Z3-5802)

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (21)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\UBC

11 of 15

Yes

Hsap\UBB

6 of 15

Yes

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (32)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Ubc

10 of 15

Yes

6 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Oas1c

1 of 15

Yes

Mmus\Oas1d

1 of 15

Yes

Mmus\Oas1e

1 of 15

Yes

Mmus\Oas1f

1 of 15

Yes

Mmus\Oas1g

1 of 15

Yes

Mmus\Oas1h

1 of 15

Yes

Mmus\Oasl1

1 of 15

Yes

Mmus\Oasl2

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Rattus norvegicus (Norway rat) (33)

Rnor\Ubc

8 of 13

Yes

Rnor\Ubb

3 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Rnor\Oas1a

1 of 13

Yes

Rnor\Oas1b

1 of 13

Yes

Rnor\Oas1d

1 of 13

Yes

Rnor\Oas1e

1 of 13

Yes

Rnor\Oas1g

1 of 13

Yes

Rnor\Oas1i

1 of 13

Yes

Rnor\Oasl2

1 of 13

Yes

Rnor\Oasl

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (15)

Xtro\ubc

6 of 12

Yes

Xtro\LOC100485835

3 of 12

Yes

Xtro\LOC105945138

2 of 12

Yes

Xtro\nedd8

2 of 12

Xtro\rps27a

2 of 12

Xtro\ubb

2 of 12

Yes

Xtro\fau

1 of 12

Xtro\LOC100216108

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (18)

Drer\ubc

9 of 15

Yes

Drer\ubb

8 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Drer\si:ch211-202a12.4

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (16)

Cele\ubq-1

10 of 15

Yes

Cele\F52C6.2

2 of 15

Yes

Cele\ned-8

2 of 15

Cele\C16C8.4

1 of 15

Yes

Cele\C16C8.5

1 of 15

Yes

Cele\C16C8.11

1 of 15

Yes

Cele\C16C8.13

1 of 15

Yes

Cele\C16C8.14

1 of 15

Yes

Cele\F14D2.11

1 of 15

Yes

Cele\F34H10.1

1 of 15

Cele\F52C6.3

1 of 15

Cele\F52C6.4

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (19)

Atha\UBQ8

5 of 9

Yes

Atha\UBQ14

5 of 9

Yes

Atha\UBQ7

3 of 9

Yes

Atha\AT1G23410

2 of 9

Atha\AT1G53930

2 of 9

Yes

Atha\RUB1

2 of 9

Yes

Atha\UBQ4

2 of 9

Yes

Atha\UBQ10

2 of 9

Yes

Atha\UBQ12

2 of 9

Yes

Atha\UBQ13

2 of 9

Yes

Atha\AT2G36170

1 of 9

Atha\AT5G09340

1 of 9

Yes

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

Yes

Atha\UBQ11

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\UBI4

10 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\ubi4

9 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091901Y0 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila simulans

Drosophila simulans

Drosophila simulans

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA30006

Drosophila pseudoobscura pseudoobscura

Dpse\GA30652

Drosophila persimilis

Dper\GL16204

Drosophila persimilis

Dper\GL20284

Drosophila persimilis

Dper\GL20619

Drosophila willistoni

Dwil\GK20481

Drosophila willistoni

Dwil\GK28304

Drosophila virilis

Dvir\GJ16000

Drosophila virilis

Dvir\GJ16068

Drosophila mojavensis

Dmoj\GI12770

Drosophila mojavensis

Dmoj\GI14272

Drosophila mojavensis

Dmoj\GI21526

Drosophila grimshawi

Dgri\GH15714

Drosophila grimshawi

Dgri\GH12661

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500RT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17022142

Musca domestica

House fly

Mdoa\17025753

Musca domestica

House fly

Mdoa\17011646

Musca domestica

House fly

Mdoa\17014836

Musca domestica

House fly

Mdoa\17017692

Glossina morsitans

Tsetse fly

Gmor\GMOY007966

Glossina morsitans

Tsetse fly

Gmor\GMOY005690

Mayetiola destructor

Hessian fly

Mdes\Mdes004661

Mayetiola destructor

Hessian fly

Mdes\Mdes009910

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL007168

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL000679

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL003888

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013536

Aedes aegypti

Yellow fever mosquito

Aaeg\Ub52

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013680

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL000795

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL008920

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL002368

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL003881

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL003877

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL011219

Anopheles darlingi

American malaria mosquito

Adar\ADAC006535

Anopheles darlingi

American malaria mosquito

Adar\ADAC006534

Anopheles darlingi

American malaria mosquito

Adar\ADAC006215

Anopheles darlingi

American malaria mosquito

Adar\ADAC009123

Anopheles gambiae

Malaria mosquito

Agam\AGAP002599

Anopheles gambiae

Malaria mosquito

Agam\AGAP001969

Anopheles gambiae

Malaria mosquito

Agam\AGAP007913

Anopheles gambiae

Malaria mosquito

Agam\AGAP013742

Anopheles gambiae

Malaria mosquito

Agam\AGAP001971

Anopheles gambiae

Malaria mosquito

Agam\Rpl40

Anopheles gambiae

Malaria mosquito

Agam\AGAP001970

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ013710

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ015770

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ013324

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003598

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003589

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002413

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003099

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ001487

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003588

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00LT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA001415

Bombyx mori

Silkmoth

Bmor\BGIBMGA001549

Bombyx mori

Silkmoth

Bmor\BGIBMGA011273

Bombyx mori

Silkmoth

Bmor\UBI1

Bombyx mori

Silkmoth

Bmor\BGIBMGA007334

Danaus plexippus

Monarch butterfly

Dple\DPOGS208502-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS204814-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS202511-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS211179-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL012420

Heliconius melpomene

Postman butterfly

Hmel\HMEL011485

Heliconius melpomene

Postman butterfly

Hmel\HMEL007444

Heliconius melpomene

Postman butterfly

Hmel\HMEL008746

Heliconius melpomene

Postman butterfly

Hmel\HMEL016506

Heliconius melpomene

Postman butterfly

Hmel\HMEL013556

Apis florea

Little honeybee

Aflo\2965419524

Apis florea

Little honeybee

Aflo\5081404820

Apis florea

Little honeybee

Aflo\2823877220

Apis florea

Little honeybee

Aflo\2147486065

Apis florea

Little honeybee

Aflo\2147485985

Apis florea

Little honeybee

Aflo\2147696803

Apis florea

Little honeybee

Aflo\3378550612

Apis mellifera

Western honey bee

Amel\RpL40

Apis mellifera

Western honey bee

Amel\LOC409675

Apis mellifera

Western honey bee

Amel\GB53968

Apis mellifera

Western honey bee

Amel\GB45661

Apis mellifera

Western honey bee

Amel\RpS27A

Apis mellifera

Western honey bee

Amel\GB53967

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP18939

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP10609

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP25239

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP11826

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP16856

Bombus terrestris

Buff-tailed bumblebee

Bter\15201502

Bombus terrestris

Buff-tailed bumblebee

Bter\15203048

Bombus terrestris

Buff-tailed bumblebee

Bter\15204858

Bombus terrestris

Buff-tailed bumblebee

Bter\15216577

Bombus terrestris

Buff-tailed bumblebee

Bter\15205336

Linepithema humile

Argentine ant

Lhum\LH16507

Linepithema humile

Argentine ant

Lhum\LH26187

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0021af

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002657

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0025f3

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0020f3

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG023157

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG011275

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG006666

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG017061

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG013235

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG031153

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_07754

Tribolium castaneum

Red flour beetle

Tcas\TC014299

Tribolium castaneum

Red flour beetle

Tcas\TC009978

Tribolium castaneum

Red flour beetle

Tcas\TC032827

Pediculus humanus

Human body louse

Phum\PHUM528640

Pediculus humanus

Human body louse

Phum\PHUM442590

Pediculus humanus

Human body louse

Phum\PHUM602270

Rhodnius prolixus

Kissing bug

Rpro\RPRC006442

Rhodnius prolixus

Kissing bug

Rpro\RPRC008770

Rhodnius prolixus

Kissing bug

Rpro\RPRC012247

Rhodnius prolixus

Kissing bug

Rpro\RPRC014620

Cimex lectularius

Bed bug

Clec\CLEC008644

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI086543

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI064888

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI066032

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005197

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000086

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007765

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003315

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000076

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008479

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_08320

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_09025

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_08554

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_02553

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_15362

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X08AW )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR005736

Strigamia maritima

European centipede

Smar\SMAR005739

Strigamia maritima

European centipede

Smar\SMAR005737

Ixodes scapularis

Black-legged tick

Isca\ISCW010407

Ixodes scapularis

Black-legged tick

Isca\ISCW023133

Ixodes scapularis

Black-legged tick

Isca\ISCW004394

Ixodes scapularis

Black-legged tick

Isca\ISCW000125

Stegodyphus mimosarum

African social velvet spider

Smim\KFM70679.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM70936.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur06g00900

Tetranychus urticae

Two-spotted spider mite

Turt\tetur17g01490

Tetranychus urticae

Two-spotted spider mite

Turt\tetur03g06910

Tetranychus urticae

Two-spotted spider mite

Turt\tetur18g03450

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_52566

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_210344

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_243700

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_17671

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_48503

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_9558

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_302125

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_48454

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G178I )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Rps27a

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Nedd8_2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ubq/RL40fp

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-UbcL

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-C19orf50L

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Polyq

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000020815

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000005834

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000002990

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000018890

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000005008

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000007605

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000004509

Gallus gallus

Domestic chicken

Ggal\RPS27A

Gallus gallus

Domestic chicken

Ggal\C19orf50

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (11)

Gene Symbol

Score

Source

Alignment

7 of 10

5 of 10

4 of 10

4 of 10

3 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Ubi-p63E^unspecified

exacerbates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Steffan et al., 2004)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

11 of 15

6 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ubi-p63E - Act88F

ion exchange chromatography, peptide massfingerprinting

(Schmitz et al., 2003)

Ubi-p63E - CG5958

split luciferase complementation, luminiscence technology

(Kolkhof et al., 2017)

Ubi-p63E - CYLD

deubiquitinase assay, molecular weight estimation by staining, anti tag western blot

(Asaoka et al., 2016, Tsichritzis et al., 2007)

Ubi-p63E - Dronc

anti tag coimmunoprecipitation, western blot, anti tag western blot

(Kamber Kaya et al., 2017)

Ubi-p63E - Dscam1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Yang et al., 2012)

Ubi-p63E - Gpat4

split luciferase complementation, luminiscence technology

(Kolkhof et al., 2017)

Ubi-p63E - Graf

pull down, western blot

(Kim et al., 2017)

Ubi-p63E - LUBEL

enzymatic study, western blot, pull down

(Asaoka et al., 2016)

Ubi-p63E - Lsd-1

split luciferase complementation, luminiscence technology

(Kolkhof et al., 2017)

Ubi-p63E - Lsd-2

split luciferase complementation, luminiscence technology

(Kolkhof et al., 2017)

Ubi-p63E - Marf

anti bait coimmunoprecipitation, western blot

(Poole et al., 2010)

Ubi-p63E - Rbf

anti tag coimmunoprecipitation, anti tag western blot

(Raj et al., 2012)

Ubi-p63E - Ufl1

split luciferase complementation, luminiscence technology

(Kolkhof et al., 2017)

Ubi-p63E - bam

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, Identification by mass spectrometry

(Ji et al., 2017)

Ubi-p63E - d

anti tag coimmunoprecipitation, western blot

(Bosch et al., 2014)

Ubi-p63E - grim

anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot

(Yeh and Bratton, 2013)

Ubi-p63E - lqf

pull down, western blot

(Xie et al., 2012)

Ubi-p63E - lwr

pull down, western blot

(Shih et al., 2002)

Ubi-p63E - morgue

anti tag coimmunoprecipitation, peptide massfingerprinting, pull down, western blot

(Zhou et al., 2013)

Ubi-p63E - rdx

anti tag coimmunoprecipitation, anti tag western blot

(Zhou et al., 2015)

Ubi-p63E - rngo

pull down, anti tag western blot, anti bait coimmunoprecipitation

(Morawe et al., 2011)

Ubi-p63E - smo

anti tag coimmunoprecipitation, covalent binding, anti tag western blot

(Yang et al., 2013)

Ubi-p63E - sturkopf

split luciferase complementation, luminiscence technology

(Kolkhof et al., 2017)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Usp14

enhanceable

Ubi-p63E

(Kovács et al., 2020)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63944

DroID - A comprehensive database of gene and protein interactions.

FBgn0003943

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

38456

MIST (protein-protein) - An integrated Molecular Interaction Database

Ubi-p63E

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-9

Cytogenetic map

63F5-63F5

Sequence location

3L:3,899,259..3,903,184 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

63F5-63F5

Limits computationally determined from genome sequence between P{PZ}kst⁰¹³¹⁸ and P{PZ}Sc2⁰⁵⁶³⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

63F2-63F4

(determined by in situ hybridisation)

(Ranz et al., 1997)

63E-63E

(determined by in situ hybridisation)

(Izquierdo, 1994)

63F-63F

(determined by in situ hybridisation)

(Arribas et al., 1986)

Experimentally Determined Recombination Data

Location

3-9

(FlyBase, 2016)

3-8.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Same map position as 63F early ecdysone-induced puff.

Stocks and Reagents

Stocks (5)

Bloomington

16814

y¹ w^67c23; P{EPgy2}Ubi-p63E^EY07341

61503

y¹ w^*; Ubi-p63E^23b/TM3, Sb¹

61502

y¹ w^*; Df(3L)Ubi-p63E^12c, Ubi-p63E^12c/TM3, Sb¹

33405

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00284}attP2/TM3, Sb¹

VDRC

v102379

P{KK109327}VIE-260B

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (95)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003943

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003943

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38456

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

The Ubi-p5E and Ubi-p63E genes share at least 50% identity.

(Langille and Clark, 2007)

The insertion in the "l(3)05634" line is within the Sc2 gene, rather than affecting Ubi-p63E as stated in FBrf0111489.

(Vaskova et al., 2000)

The insertion in the "l(3)05634" line is stated to affect Ubi-p63E in this paper. A subsequent publication suggests that this is incorrect and the insertion is in fact in Sc2 (see FBrf0130136).

(Spradling et al., 1999)

Other Comments

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Area matching Drosophila Ubiquitin gene, Acc. No. M22428.

(Davies, 2001.3.15)

Antibody staining of polytene chromosomes shows that ubiquitin is mainly associated with the compact and stabilized structure that forms the bands rather than with the more decondensed and destabilized protein-DNA structure that forms interbands and puffs.

(Izquierdo, 1994)

Ubi-p63E expression has been studied in heat shocked aging flies.

(Niedzwiecki and Fleming, 1993)

Structural gene for ubiquitin (Arribas, Sampedro and Izquierdo, 1988); structure and expression described by Lee, Simon and Lis (1988). Threefold increase in level of gene by heat shock. A Ubi-p63E-lacZ fusion gene introduced by germ line transformation has been expressed at high levels at all life stages tested (embryonic, larval, pupal and adult).

Origin and Etymology

Discoverer

Etymology

Named 'magellan' because mutant spermatocytes arrest development with abnormal structures that resemble Magellanic clouds.

(Lu et al., 2013)

Identification

External Crossreferences and Linkouts ( 207 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38456

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P0CG69

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003943

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11624

Flygut - An atlas of the Drosophila adult midgut

FBgn0003943

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38456

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG11624

modMine - A data warehouse for the modENCODE project

FBgn0003943

PDB - An information portal to biological macromolecular structures

Linkouts

BioGRID - A database of protein and genetic interactions.

63944

DroID - A comprehensive database of gene and protein interactions.

FBgn0003943

DRSC - Results frm RNAi screens

FBgn0003943

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0003943

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG11624-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003943

FlyMine - An integrated database for Drosophila genomics

FBgn0003943

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

38456

MIST (protein-protein) - An integrated Molecular Interaction Database

Ubi-p63E

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (28)

Reported As

Symbol Synonym

CG11624

(Ristic et al., 2016, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Hughes et al., 2008, Qin et al., 2005, Ramet et al., 2002, Fulton et al., 2001)

DmUb

(Shih et al., 2002)

DmUbi-p63E

(Hoe et al., 2011)

(Chi et al., 2016, Poole et al., 2010)

(Nagy et al., 2018, Ji et al., 2017, Kim et al., 2017, Zhou et al., 2015, Bosch et al., 2014, Chang et al., 2013, Yang et al., 2013, Yeh and Bratton, 2013, Zhou et al., 2013, Raj et al., 2012, Tsichritzis et al., 2007, Koh et al., 2006, Koh et al., 2006, Moberg et al., 2005, Kanuka et al., 2003, Huang et al., 1995)

Ub-p63E

(Kanoh et al., 2015)

Ubi-P63e

(Qin et al., 2005)

Ubi-p

(Vaskova et al., 2000, Lindsley and Zimm, 1992)

Ubi-p63E

(Zhang and Zhang, 2019, Geronikolou et al., 2018, Asaoka et al., 2016, Brewer-Jensen et al., 2016, Cruz-Becerra et al., 2016, Yu et al., 2016, Dent et al., 2015, Lu et al., 2013, Neumüller et al., 2013, Munoz-Soriano et al., 2012, Fisher et al., 2008, Langille and Clark, 2007, Marygold, 2007.1.12, Zhang et al., 2007, Ma et al., 2006, Stroschein-Stevenson et al., 2006, Ulvila et al., 2006, Lawniczak and Begun, 2004, Gim et al., 2001)

Ubi63E

(Bernards and Hariharan, 2001, Fernandez-Funez et al., 2000)

Ubi63p

(Stielow et al., 2008)

Ubip

(Maroni, 1994)

Ubip63E

(Munoz-Soriano et al., 2012)

Ubiq

(Wesley and Mok, 2003)

Ubp

(Fischer-Vize et al., 1997)

magn

(Lu et al., 2013)

ubi

(Witzberger et al., 2008)

ubi-p63E

(Zhou et al., 2008)

ubi63E

(Ryoo et al., 2002, Ryoo et al., 2002)

Name Synonyms

Ubiquitin

(Kolkhof et al., 2017, Herz et al., 2006, Wesley and Mok, 2003, Zoghbi and Botas, 2002, Noll et al., 2000, Pham and Sauer, 2000)

Ubiquitin 63E

(Bardet et al., 2013)

Ubiquitin-63-E

(Yang et al., 2012)

Ubiquitin-63E

(Urban et al., 2017, Nybakken et al., 2005, Qin et al., 2005, Gim et al., 2001)

Ubiquitin-polymeric

(Lindsley and Zimm, 1992)

magellan

(Lu et al., 2013)

polyubiquitin

(Lu et al., 2013, Niedzwiecki and Fleming, 1993)

ubiquitin

(Xu et al., 2017, Chang et al., 2013, Morawe et al., 2011, Simonsen et al., 2007, Lu and Bilder, 2005, Moberg et al., 2005, Park et al., 2005, Sakata et al., 2004, Finley et al., 2003, Schmitz et al., 2003, Auluck et al., 2002, Murphey and Godenschwege, 2002)

ubiquitin-63E

(Geronikolou et al., 2018)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (289)

The following external sites may use different assemblies or annotations than FlyBase.

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