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Gene Dmel\Vm26Ab

General Information
Symbol	Dmel\Vm26Ab	Species	D. melanogaster
Name	Vitelline membrane 26Ab	Annotation symbol	CG9046
Feature type	protein_coding_gene	FlyBase ID	FBgn0003980
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	sV23, VM26A.2, fs(2)QJ42
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	26A3-26A3	Sequence location	2L:5,957,004..5,957,628 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Vitelline membrane 26Ab is referred to in FlyBase by the symbol Dmel\Vm26Ab (CG9046, FBgn0003980). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: structural constituent of vitelline membrane. There is experimental evidence that it is involved in the biological process: chorion-containing eggshell formation; egg activation. 7 alleles are reported. The phenotype of these alleles is annotated with: endochorion. It has one annotated transcript and one annotated polypeptide. Protein features are: Vitelline membrane cysteine-rich domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of extremely low expression. Peak expression observed in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1641651_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Vm26Ab. Gene sequence location is 2L:5957004..5957628.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features BKN31612 HFA03473
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 26A3-26A3   Limits computationally determined from genome sequence between P{EP}bchsEP2299&P{EP}GpdhEP466 and P{lacW}chick13321
Experimentally Determined Cytological Location
Cytogenetic map Notes References 26A-26A   (determined by in situ hybridisation)   (Whiting et al., 1989) 26A-26A   (determined by in situ hybridisation)   (Burke et al., 1987)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Vm26Ab for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Vm26Ab-RA FBtr0079171  NM_057436   625   168
Additional Transcript Data & Comments
Reported size (kB)	0.7 (northern blot) (Popodi et al., 1988)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Vm26Ab-PA   FBpp0078802   16.8   168   8.00     NP_476784   AAF52308
Additional Polypeptide Data & Comments
Reported size (kDa)	168 (aa); 16.595 (kD predicted) (Popodi et al., 1988)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Vitelline membrane cysteine-rich domain (IPR013135)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014134 AAF52308   AC004268   AC004758   AC092239   BT023245   BT029421   BT031036   EV575430   EV576614   EV577349   EV577615   EV578125   EV579047   EV579964   EV580846   EV581385   EV581542   EV582185   EV582507   EV582743   EV583660   EV584103   EV586038   EV592005   M20936 AAA28984
UniProtKB/Swiss-Prot	P13238
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference adult stage & oogenesis   follicle cell   (Popodi et al., 1988) oogenesis stage S8 -- S11     (Popodi et al., 1988)
Additional Descriptive Data
	Vm26Ab is first detected in stage S8-S9 egg chambers, and persists until stage S11. Expression is follicle cell-specific. (Popodi et al., 1988)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   vitelline membrane   (Pascucci et al., 1996) oogenesis stage S9 -- S14     (Pascucci et al., 1996)
Additional Descriptive Data
	The mobility of the Vm26Ab protein on Western blots shifts from a sharp 23 kD band at stage S9-S10A to a smear with a leading edge at 21 kD (up to stage S13) and later at 17 kD (stage S13-S14). In stage S10 egg chambers,Vm26Ab protein localizes to the vitelline membrane. (Pascucci et al., 1996)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0003980    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0003980 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of extremely low expression. Peak expression observed in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0003980 expression level Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    23 embryo 04-06hr    29 embryo 06-08hr    42 embryo 08-10hr    21 embryo 10-12hr    11 embryo 12-14hr    11 embryo 14-16hr    27 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    17 embryo 22-24hr    15 larva L1    3 larva L2    1 larva L3 12hr old    4 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    9 white prepupae 12hr    56 white prepupae 24hr    36 pupae 2d postWPP    29 pupae 3d postWPP    19 pupae 4d postWPP    149 adult male 01day    445 adult male 05day    29 adult male 30day    40 adult female 01day    111036 adult female 05day    236989 adult female 30day    210886 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    23 embryo 04-06hr    29 embryo 06-08hr    42 embryo 08-10hr    21 embryo 10-12hr    11 embryo 12-14hr    11 embryo 14-16hr    27 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    17 embryo 22-24hr    15 larva L1    3 larva L2    1 larva L3 12hr old    4 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    9 white prepupae 12hr    56 white prepupae 24hr    36 pupae 2d postWPP    29 pupae 3d postWPP    19 pupae 4d postWPP  (149) adult male 01day  (445) adult male 05day    29 adult male 30day    40 adult female 01day  (111036) adult female 05day  (236989) adult female 30day  (210886) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    23 embryo 04-06hr    29 embryo 06-08hr    42 embryo 08-10hr    21 embryo 10-12hr    11 embryo 12-14hr    11 embryo 14-16hr    27 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    17 embryo 22-24hr    15 larva L1    3 larva L2    1 larva L3 12hr old    4 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    9 white prepupae 12hr    56 white prepupae 24hr    36 pupae 2d postWPP    29 pupae 3d postWPP    19 pupae 4d postWPP    149 adult male 01day    445 adult male 05day    29 adult male 30day    40 adult female 01day  (111036) adult female 05day  (236989) adult female 30day  (210886) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    23 embryo 04-06hr    29 embryo 06-08hr    42 embryo 08-10hr    21 embryo 10-12hr    11 embryo 12-14hr    11 embryo 14-16hr    27 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    17 embryo 22-24hr    15 larva L1    3 larva L2    1 larva L3 12hr old    4 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    9 white prepupae 12hr    56 white prepupae 24hr    36 pupae 2d postWPP    29 pupae 3d postWPP    19 pupae 4d postWPP    149 adult male 01day    445 adult male 05day    29 adult male 30day    40 adult female 01day  (111036) adult female 05day  (236989) adult female 30day  (210886) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    23 embryo 04-06hr    29 embryo 06-08hr    42 embryo 08-10hr    21 embryo 10-12hr    11 embryo 12-14hr    11 embryo 14-16hr    27 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    17 embryo 22-24hr    15 larva L1    3 larva L2    1 larva L3 12hr old    4 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    9 white prepupae 12hr    56 white prepupae 24hr    36 pupae 2d postWPP    29 pupae 3d postWPP    19 pupae 4d postWPP    149 adult male 01day    445 adult male 05day    29 adult male 30day    40 adult female 01day    111036 adult female 05day    236989 adult female 30day    210886 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0003980 expression level Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    23 embryo 04-06hr    29 embryo 06-08hr    42 embryo 08-10hr    21 embryo 10-12hr    11 embryo 12-14hr    11 embryo 14-16hr    27 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    17 embryo 22-24hr    15 larva L1    3 larva L2    1 larva L3 12hr old    4 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    9 white prepupae 12hr    56 white prepupae 24hr    36 pupae 2d postWPP    29 pupae 3d postWPP    19 pupae 4d postWPP    149 adult male 01day    445 adult male 05day    29 adult male 30day    40 adult female 01day    111036 adult female 05day    236989 adult female 30day    210886 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    23 embryo 04-06hr    29 embryo 06-08hr    42 embryo 08-10hr    21 embryo 10-12hr    11 embryo 12-14hr    11 embryo 14-16hr    27 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    17 embryo 22-24hr    15 larva L1    3 larva L2    1 larva L3 12hr old    4 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    9 white prepupae 12hr    56 white prepupae 24hr    36 pupae 2d postWPP    29 pupae 3d postWPP    19 pupae 4d postWPP  (149) adult male 01day  (445) adult male 05day    29 adult male 30day    40 adult female 01day  (111036) adult female 05day  (236989) adult female 30day  (210886) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    23 embryo 04-06hr    29 embryo 06-08hr    42 embryo 08-10hr    21 embryo 10-12hr    11 embryo 12-14hr    11 embryo 14-16hr    27 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    17 embryo 22-24hr    15 larva L1    3 larva L2    1 larva L3 12hr old    4 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    9 white prepupae 12hr    56 white prepupae 24hr    36 pupae 2d postWPP    29 pupae 3d postWPP    19 pupae 4d postWPP    149 adult male 01day    445 adult male 05day    29 adult male 30day    40 adult female 01day  (111036) adult female 05day  (236989) adult female 30day  (210886) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    23 embryo 04-06hr    29 embryo 06-08hr    42 embryo 08-10hr    21 embryo 10-12hr    11 embryo 12-14hr    11 embryo 14-16hr    27 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    17 embryo 22-24hr    15 larva L1    3 larva L2    1 larva L3 12hr old    4 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    9 white prepupae 12hr    56 white prepupae 24hr    36 pupae 2d postWPP    29 pupae 3d postWPP    19 pupae 4d postWPP    149 adult male 01day    445 adult male 05day    29 adult male 30day    40 adult female 01day  (111036) adult female 05day  (236989) adult female 30day  (210886) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    23 embryo 04-06hr    29 embryo 06-08hr    42 embryo 08-10hr    21 embryo 10-12hr    11 embryo 12-14hr    11 embryo 14-16hr    27 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    17 embryo 22-24hr    15 larva L1    3 larva L2    1 larva L3 12hr old    4 larva L3 puffstage 1-2    33 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    9 white prepupae 12hr    56 white prepupae 24hr    36 pupae 2d postWPP    29 pupae 3d postWPP    19 pupae 4d postWPP    149 adult male 01day    445 adult male 05day    29 adult male 30day    40 adult female 01day    111036 adult female 05day    236989 adult female 30day    210886 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0003980    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0003980 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0003980 expression level Tissue   Expression Level Larval Central Nervous System    9.05 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.3 Larval Fat Body    8.9 Larval Salivary Gland no informative data Larval Trachea    6.05 Larval Carcass no informative data Adult Head    7.8 Adult Eye    5.325 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    6.3 Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    35.5 Adult Salivary Gland no informative data Adult Heart    8.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    2542.3 Adult Testis    7.2 Adult Male Accessory Gland no informative data Adult Carcass    253.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    9.05 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.3 Larval Fat Body    8.9 Larval Salivary Gland no informative data Larval Trachea    6.05 Larval Carcass no informative data Adult Head    7.8 Adult Eye    5.325 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    6.3 Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    35.5 Adult Salivary Gland no informative data Adult Heart    8.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary  (2542.3) Adult Testis    7.2 Adult Male Accessory Gland no informative data Adult Carcass    253.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    9.05 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.3 Larval Fat Body    8.9 Larval Salivary Gland no informative data Larval Trachea    6.05 Larval Carcass no informative data Adult Head    7.8 Adult Eye    5.325 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    6.3 Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    35.5 Adult Salivary Gland no informative data Adult Heart    8.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary  (2542.3) Adult Testis    7.2 Adult Male Accessory Gland no informative data Adult Carcass    253.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    9.05 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.3 Larval Fat Body    8.9 Larval Salivary Gland no informative data Larval Trachea    6.05 Larval Carcass no informative data Adult Head    7.8 Adult Eye    5.325 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    6.3 Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    35.5 Adult Salivary Gland no informative data Adult Heart    8.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    2542.3 Adult Testis    7.2 Adult Male Accessory Gland no informative data Adult Carcass    253.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0003980 expression level Tissue   Expression Level Larval Central Nervous System    9.05 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.3 Larval Fat Body    8.9 Larval Salivary Gland no informative data Larval Trachea    6.05 Larval Carcass no informative data Adult Head    7.8 Adult Eye    5.325 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    6.3 Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    35.5 Adult Salivary Gland no informative data Adult Heart    8.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    2542.3 Adult Testis    7.2 Adult Male Accessory Gland no informative data Adult Carcass    253.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    9.05 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.3 Larval Fat Body    8.9 Larval Salivary Gland no informative data Larval Trachea    6.05 Larval Carcass no informative data Adult Head    7.8 Adult Eye    5.325 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    6.3 Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    35.5 Adult Salivary Gland no informative data Adult Heart    8.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary  (2542.3) Adult Testis    7.2 Adult Male Accessory Gland no informative data Adult Carcass    253.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    9.05 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.3 Larval Fat Body    8.9 Larval Salivary Gland no informative data Larval Trachea    6.05 Larval Carcass no informative data Adult Head    7.8 Adult Eye    5.325 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    6.3 Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    35.5 Adult Salivary Gland no informative data Adult Heart    8.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary  (2542.3) Adult Testis    7.2 Adult Male Accessory Gland no informative data Adult Carcass    253.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    9.05 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules    7.3 Larval Fat Body    8.9 Larval Salivary Gland no informative data Larval Trachea    6.05 Larval Carcass no informative data Adult Head    7.8 Adult Eye    5.325 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut    6.3 Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    35.5 Adult Salivary Gland no informative data Adult Heart    8.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    2542.3 Adult Testis    7.2 Adult Male Accessory Gland no informative data Adult Carcass    253.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland no informative data Larval Trachea     Larval Carcass no informative data Adult Head     Adult Eye     Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut     Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    7.8   NA  Eye    5.325   NA  Brain    no informative data   9.05  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    no informative data   no informative data  Midgut    6.3   no informative data  Hindgut    no informative data   7.3  Malpighian Tubules    no informative data   8.9  Fat Body    35.5   no informative data  Salivary Gland    no informative data   NA  Heart    8.4   6.05  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    2542.3   NA  Testis    7.2   NA  Male Accessory Gland    no informative data   no informative data  Carcass    253.5 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_00
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003980 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9046-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Sterility Allele female sterile Vm26AbQJ42 Phenotype manifest in Allele endochorion Vm26AbQJ42
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Vm26Ab Class Mutagen Stocks Known lesion Vm26AbQJ42 ethyl methanesulfonate 2 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Vm26Ab Class Mutagen Stocks Known lesion Vm26AbGD5254 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Vm26AbKK111612 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Vm26AbΔC.117-167 in vitro construct - deletion 0 Yes Vm26AbΔC.140-167 in vitro construct - deletion 0 Yes Vm26AbΔC.155-167 in vitro construct - deletion 0 Yes Vm26AbΔN.24-42 in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)BSC168 (Christensen and Cook, 2006.8.29) Df(2L)BSC183 (Christensen and Cook, 2006.8.29) Df(2L)BSC353 (Christensen et al., 2007.10.29) Df(2L)cl7 (Savant and Waring, 1989) Df(2L)Exel7024 (Ryder, 2004.4.26)
Duplicated in	Df(2L)BSC168 (Christensen and Cook, 2006.8.29)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD5254} NA P{KK111612} NA characterization construct P{Vm26AbΔC.140-167} NA P{Vm26AbΔC.155-167} NA P{Vm26AbΔN.24-42} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
chorion-containing eggshell formation inferred from mutant phenotype (Schupbach and Wieschaus, 1991) egg activation inferred from mutant phenotype (Tadros et al., 2003)
Cellular Component
CV term Evidence References
vitelline envelope inferred from direct assay (Elalayli et al., 2008)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
structural constituent of vitelline membrane non-traceable author statement (FlyBase, 1992-, Swiss-Prot Project Members, 1990.1.1)
Biological Process
CV term Evidence References
vitelline membrane formation involved in chorion-containing eggshell formation non-traceable author statement (Lindsley and Zimm, 1992)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Vm26Ab allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 59992 DroID - A comprehensive database of gene and protein interactions. • FBgn0003980 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33827
Orthologs
Genome-wide drosophilid orthologs	Dana\GF15591 Dere\GG25107 Dgri\GH11015 Dmoj\GI17534 Dper\GL19046 Dpse\GA21501 Dsec\GM18584 Dsim\GD23372 Dvir\GJ15426 Dwil\GK14709 Dyak\GE25756
Curated drosophilid orthologs	Dpse\GA21501   Dvir\Vm26Ab   Dyak\Vm26Ab
Linkouts
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	5118 Vm26AbQJ42 cn1 bw1/CyO, l(2)DTS5131
Kyoto	108051 Vm26AbQJ42 cn1 bw1/CyO, l(2)DTS5131
VDRC	v103228 P{KK111612}VIE-260B v13230 w1118; P{GD5254}v13230/TM3
Genomic Clones ( 1 )
	BACR48E14 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 20 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP02754 IP02756
Other clones	TA01352
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	Dmel_dig_000417_0688_0343 Dmel_dig_001693_0622_1620 Dmel_dig_007684_0698_1714 Dmel_dig_008202_0627_0288 Dmel_dig_008346_0591_2236 Dmel_dig_010095_0668_3169 Dmel_dig_012267_0612_0320 Dmel_dig_012467_0654_1525 Dmel_dig_013604_0616_2533 Dmel_dig_013704_0668_1256 Dmel_dig_013968_0643_1492 Dmel_dig_014344_0693_2007 Dmel_dig_015393_0690_2744 Dmel_dig_015475_0710_1652 Dmel_dig_016416_0655_3429 Dmel_dig_017014_0660_2308 Dmel_neb_011832_0568_3439
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003980 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33827
Antibody Information
	polyclonal (Pascucci et al., 1996)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes. (Kliman and Eyre-Walker, 1998) Polyclonal antibodies have been used to localise Vm26Aa, Vm26Ab, Cp18 and Cp36 in the assembly and mature eggshell. Vitelline membrane proteins are processed in a stage specific manner, morphological integrity of the outer chorion layers is dependent upon proper expression of at least one vitelline membrane component, sV23. (Pascucci et al., 1996) The Vm26Aa, Vm26Ab, Vm32E, Vm34Ca genes make up a multigene family that includes at least these four members. (Lindsley and Zimm, 1992) The Vm26Aa, Vm26Ab, Vm32E, Vm34Ca genes are responsible for encoding the major proteins of the first layer of the Drosophila eggshell, the vitelline membrane. (Lindsley and Zimm, 1992) Vm26Aa, Vm26Ab, Vm32E, Vm34Ca encode translation products with the high proline and alanine content characteristic of vitelline membrane proteins. (Gigliotti et al., 1989, Mindrinos et al., 1985) In situ hybridisation of recombinant DNA probes to polytene chromosomes of D.melanogaster and D.virilis is performed to study homologies between the chromosomal Muller elements of the two species. (Whiting et al., 1989)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057436 RefSeq (Proteins) • NP_476784 DDBJ / EMBL / GenBank • AAA28984 AAF52308 AC004268 AC004758 AC092239 M20936 Entrez Gene - A searchable database of RefSeq genes. • 33827 UniProtKB/Swiss-Prot • P13238
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Vitelline membrane cysteine-rich domain (IPR013135)
Linkouts
	BioGRID - A database of protein and genetic interactions • 59992 DroID - A comprehensive database of gene and protein interactions. • FBgn0003980 DRSC - Results from RNAi screens. • FBgn0003980 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003980 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9046-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003980 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33827 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33827 modMine - Data generated by the modENCODE project. • FBgn0003980
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	CG9046 (Keleman et al., 2009.8.5) Dm sV23 (Fokta, 2001) fs(2)QJ42 (Cernilogar et al., 2001, Andrenacci et al., 2001, Pascucci et al., 1996, ) sV23 (Wu and Waring, 2005, Greene and Waring, 2005, Manogaran and Waring, 2004, Waring et al., 2004, Manogaran et al., 2003, Manogaran et al., 2003, Anonymous, 2001, Heifetz et al., 2001, Manogaran et al., 2001, Fokta and Waring, 1997, Pascucci et al., 1996, LeMosy et al., 2006, Lee and Cooley, 2006, Fakhouri et al., 2006, Wu and Waring, 2008, Elalayli et al., 2008, Ventura et al., 2010, Zappia et al., 2011, Trougakos et al., 2001) Sv23 (LeMosy and Hashimoto, 2000, Hong and Hashimoto, 1995) sv23   sV26 (Pascucci et al., 1996) TU-4 (Popodi et al., 1988, ) Vm26A (Lin et al., 1993) VM26A.2 (Cernilogar et al., 2001, Andrenacci et al., 2001, Bernardi et al., 2006, Cavaliere et al., 2008, Bernardi et al., 2009) Vm26Ab (Baker and Russell, 2009, Christensen and Cook, 2006.8.29, Christensen and Cook, 2006.8.29, Christensen et al., 2007.10.29, Jagadeeshan and Singh, 2007, Zhang et al., 2009, Alatortsev, 2006, Lai et al., 2007, Wu et al., 2010)
Name Synonym	Vitelline membrane 26Ab (McGraw et al., 2008)
Secondary FlyBase IDs
References ( 63 )
Generate a list of	All references relating to this gene ( 63 )
List References by type	Research paper  ( 35 ) Supplementary material  ( 2 ) Personal communication to FlyBase  ( 6 ) Abstract  ( 14 ) FlyBase analysis  ( 2 ) Book  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 3 )
	Zappia et al., 2011, PLoS ONE 6(5): e19715 M6 membrane protein plays an essential role in Drosophila oogenesis. [FBrf0213770] Ventura et al., 2010, Dev. Biol. 344(1): 224--232 closca, a new gene required for both Torso RTK activation and vitelline membrane integrity. Germline proteins contribute to Drosophila eggshell composition. [FBrf0211365] Wu et al., 2010, Dev. Biol. 347(2): 360--368 Drosophila vitelline membrane assembly: A critical role for an evolutionarily conserved cysteine in the "VM domain" of sV23. [FBrf0212042] Show all research papers ...