Symbol Dmel\ vnSpecies D. melanogaster Name vein Annotation symbol CG10491 Feature type protein_coding_gene FlyBase ID FBgn0003984 Gene Model Status Current Stock availability 15 publicly
available Also Known As vein, CT29452 Chromosome (arm) 3L Recombination map 3-16.2 Cytogenetic map 64E12-64F2 Sequence location 3L:5,806,438..5,838,764 [-]
Genomic Maps
Automatically generated summary
The gene
vein is referred to in FlyBase by the symbol
Dmel\vn (CG10491, FBgn0003984). It is a protein_coding_gene from
Drosophila melanogaster . There is experimental evidence that it has the
molecular function : heparin binding; epidermal growth factor receptor binding. There is experimental evidence for 12 unique
biological process terms, many of which group under: single-organism developmental process; system development; post-embryonic organ morphogenesis; appendage development; biological regulation; multicellular organismal development; positive regulation of cell proliferation; cellular component organization or biogenesis; localization; cellular process.
64 alleles are reported . The
phenotypes of these alleles are annotated with: organ system subdivision; organ system; adult segment; thoracic segment; non-connected developing system; imaginal precursor; embryonic/larval hypodermal muscle; sense organ; portion of tissue; embryonic/larval neuron; external compound sense organ. It has
2 annotated transcripts and
2 annotated polypeptides .
Protein features are: EGF-like, conserved site; Epidermal growth factor-like domain; Immunoglobulin I-set; Immunoglobulin subtype 2; Immunoglobulin-like domain; Immunoglobulin-like fold. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed at stages throughout embryogenesis, during late pupal stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1634520_at completely aligns to an exonic region of only a subset of 1 of the 2 FlyBase-annotated transcript isoforms of vn. Gene sequence location is
3L:5806438..5838764 .
User Contributed Data External Summaries
Gene/Allele symbols may differ
from current usage ddd: defective dorsal discs (A. Shearn)
Homozygous larvae perish between the first larval
instar and the prepupal stage; dorsal thoracic imaginal
discs, i.e. of the pronotum, mesonotum, and metanotum reduced
to 3% or less of normal size; all other imaginal discs develop
normally. Mutant larvae support the growth of wild-type wing
discs; mutant wing discs show very little development in
wild-type larval. Mutant cells develop normally in wing discs
that contain mixtures of mutant and wild-type cells, as produced by nuclear or cellular transplantation into blastoderms
or by somatic exchange. Mutant leg discs transplanted into
wild-type hosts can transdetermine to wing development. Studies of temperature-sensitive genotypes indicate that ddd+
product is not required for normal wing development during
embryogenesis. No evidence for a maternal effect in either
conditional mutants raised under permissive conditions and
switched to restrictive temperatures or in germ-line-transplants of mutant cells into wild-type hosts.
vn: vein
vn: vein
Large section of vein L4 and anterior crossvein
missing. Posterior crossvein often incomplete; gap sometimes
present in L3. Male sterile, female fertile.
*Vn: Vein
Vein L4 not complete. Wings slightly spread. Fly
smaller than normal. Homozygous lethal. RK2A.
Description
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FB2013_03
FB2013_02
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FlyBase Computed Cytological Location
Experimentally Determined Cytological Location
64F1-64F3
(determined by in situ hybridisation)
64F-64F
(determined by in situ hybridisation)
64F-64F
(determined by in situ hybridisation)
64F-64F
(determined by in situ hybridisation)
64F-64F
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location Left of (cM) Right of (cM) Notes 3-18.0 based on
vn allele.
Comments on Gene
Model Gene model reviewed during 5.45
Low-frequency RNA-seq exon junction(s) not annotated.
Annotated Transcripts Additional Transcript Data
& Comments Reported size (kB) Comments External Data Crossreferences Annotated
Polypeptides Additional Polypeptide
Data & Comments Reported size (kDa) 622, 621 (aa); 70 (kD observed); 71 (kD predicted)
Comments vn proteins have a number of features that
suggest function. They have a signal sequence, a PEST domain, an Ig-like
domain, and an EGF-like domain.
vn protein was shown to be a secreted.
The
vn antibody recognizes both proteins.
External Data Linkouts Crossreferences
domains - A database of protein families, domains, and functional sites
DDBJ / EMBL / GenBank UniProtKB/Swiss-Prot UniProtKB/TrEMBL Mapped Features have been reorganized, please
see this article for details. Additional mapped features and mutations can
be found on GBrowse or related reports. regulatory region
evidence=Reporter construct (in vivo)
regulatory region
evidence=Reporter construct (in vivo)
regulatory region
evidence=Reporter construct (in vivo)
Linkouts Crossreferences Comment: 1-2 cells/hemisegment
Comment: 1-2 cells/hemisegment
Additional Descriptive Data
vn transcripts are expressed inblastoderm embryos in two ventrolateral stripes that come to the midlineas gastrulation proceeds. Expression persists in the midline but isincreasingly restricted to single cells. Expression is seen in the CNS andepidermis at germ band retraction.
vn is also expressed in theamnioserosa precursors in the blastoderm and in the amnioserosa properuntil the end of germ band extension. Late in germ band extension,expression is seen in some PNS precursors which include Keilin's organprecursors and a subset of cells of the chordotonal organs.
vn is alsoexpressed in the head throughout development; in the clypeolabrum, themaxillary and labial lobes and around the stomodeum. In late embryos,expression occurs in the segmental muscles and gut wall. Transientexpression is observed in the tracheal pits at stage 10.
vn transcripts are expressed in a complex and dynamic pattern in the wing, haltere, leg and eye-antenna discs. The pattern in the wing is described in detail.
vn transcripts are first expressed in the presumptive notum and then in the wing pouch and hinge regions. Later, transcripts localize to intervein regions. This begins as a stripe of expression stradling the AP boundary in late larval discs and develops into all intervein regions after puparium formation.
Transcripts are detected in pioneer MP2 neurons in the CNS, first appearing at stage 11 and persisting at least through stage 14. Transcript is detected in the dMP2 neurons throughout this time, however, expression in the vMP2 neuron only persists through stage 12 in many cases. Transcript is also detected in VUMs and two more cells in each hemisegment.
Marker for Subcellular Localization CV Term
Additional Descriptive Data
vn protein expression in embryonic somatic muscle precursor cells colocalizes with
Kr protein expression.
Marker for Subcellular Localization (GO Cellular Component) Comment: reference states 18-20 hr APF
Associated Tools
Reference
Linear, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
26.025
Larval Midgut
13.7
Larval Hindgut
24.2
Larval Malpighian Tubules
42
Larval Fat Body
2.5
Larval Salivary Gland
3.1
Larval Trachea
47.7
Larval Carcass
32.375
Adult Head
21.1
Adult Eye
79.1
Adult Brain
8.2
Adult Thoracic-Abdominal Ganglion
10.5
Adult Crop
42.2
Adult Midgut
7.5
Adult Hindgut
18
Adult Malpighian Tubules
36.5
Adult Fat Body
11.6
Adult Salivary Gland
4.5
Adult Heart
50.475
Adult VirginFemale Spermatheca
8.5
Adult InseminatedFemale Spermatheca
9.4
Adult Ovary
8.3
Adult Testis
5.1
Adult Male Accessory Gland
8.8
Adult Carcass
15.7
Expression Level Scale
None
Low
Linear, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
26.025
Larval Midgut
13.7
Larval Hindgut
24.2
Larval Malpighian Tubules
42
Larval Fat Body
2.5
Larval Salivary Gland
3.1
Larval Trachea
47.7
Larval Carcass
32.375
Adult Head
21.1
Adult Eye
79.1
Adult Brain
8.2
Adult Thoracic-Abdominal Ganglion
10.5
Adult Crop
42.2
Adult Midgut
7.5
Adult Hindgut
18
Adult Malpighian Tubules
36.5
Adult Fat Body
11.6
Adult Salivary Gland
4.5
Adult Heart
50.475
Adult VirginFemale Spermatheca
8.5
Adult InseminatedFemale Spermatheca
9.4
Adult Ovary
8.3
Adult Testis
5.1
Adult Male Accessory Gland
8.8
Adult Carcass
15.7
Expression Level Scale
None
Low
Moderate
High
Linear, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
26.025
Larval Midgut
13.7
Larval Hindgut
24.2
Larval Malpighian Tubules
42
Larval Fat Body
2.5
Larval Salivary Gland
3.1
Larval Trachea
47.7
Larval Carcass
32.375
Adult Head
21.1
Adult Eye
79.1
Adult Brain
8.2
Adult Thoracic-Abdominal Ganglion
10.5
Adult Crop
42.2
Adult Midgut
7.5
Adult Hindgut
18
Adult Malpighian Tubules
36.5
Adult Fat Body
11.6
Adult Salivary Gland
4.5
Adult Heart
50.475
Adult VirginFemale Spermatheca
8.5
Adult InseminatedFemale Spermatheca
9.4
Adult Ovary
8.3
Adult Testis
5.1
Adult Male Accessory Gland
8.8
Adult Carcass
15.7
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
26.025
Larval Midgut
13.7
Larval Hindgut
24.2
Larval Malpighian Tubules
42
Larval Fat Body
2.5
Larval Salivary Gland
3.1
Larval Trachea
47.7
Larval Carcass
32.375
Adult Head
21.1
Adult Eye
79.1
Adult Brain
8.2
Adult Thoracic-Abdominal Ganglion
10.5
Adult Crop
42.2
Adult Midgut
7.5
Adult Hindgut
18
Adult Malpighian Tubules
36.5
Adult Fat Body
11.6
Adult Salivary Gland
4.5
Adult Heart
50.475
Adult VirginFemale Spermatheca
8.5
Adult InseminatedFemale Spermatheca
9.4
Adult Ovary
8.3
Adult Testis
5.1
Adult Male Accessory Gland
8.8
Adult Carcass
15.7
Expression Level Scale
Very high
log, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
26.025
Larval Midgut
13.7
Larval Hindgut
24.2
Larval Malpighian Tubules
42
Larval Fat Body
2.5
Larval Salivary Gland
3.1
Larval Trachea
47.7
Larval Carcass
32.375
Adult Head
21.1
Adult Eye
79.1
Adult Brain
8.2
Adult Thoracic-Abdominal Ganglion
10.5
Adult Crop
42.2
Adult Midgut
7.5
Adult Hindgut
18
Adult Malpighian Tubules
36.5
Adult Fat Body
11.6
Adult Salivary Gland
4.5
Adult Heart
50.475
Adult VirginFemale Spermatheca
8.5
Adult InseminatedFemale Spermatheca
9.4
Adult Ovary
8.3
Adult Testis
5.1
Adult Male Accessory Gland
8.8
Adult Carcass
15.7
Expression Level Scale
None
Low
Moderate
log, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
26.025
Larval Midgut
13.7
Larval Hindgut
24.2
Larval Malpighian Tubules
42
Larval Fat Body
2.5
Larval Salivary Gland
3.1
Larval Trachea
47.7
Larval Carcass
32.375
Adult Head
21.1
Adult Eye
79.1
Adult Brain
8.2
Adult Thoracic-Abdominal Ganglion
10.5
Adult Crop
42.2
Adult Midgut
7.5
Adult Hindgut
18
Adult Malpighian Tubules
36.5
Adult Fat Body
11.6
Adult Salivary Gland
4.5
Adult Heart
50.475
Adult VirginFemale Spermatheca
8.5
Adult InseminatedFemale Spermatheca
9.4
Adult Ovary
8.3
Adult Testis
5.1
Adult Male Accessory Gland
8.8
Adult Carcass
15.7
Expression Level Scale
None
Low
Moderate
High
log, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
26.025
Larval Midgut
13.7
Larval Hindgut
24.2
Larval Malpighian Tubules
42
Larval Fat Body
2.5
Larval Salivary Gland
3.1
Larval Trachea
47.7
Larval Carcass
32.375
Adult Head
21.1
Adult Eye
79.1
Adult Brain
8.2
Adult Thoracic-Abdominal Ganglion
10.5
Adult Crop
42.2
Adult Midgut
7.5
Adult Hindgut
18
Adult Malpighian Tubules
36.5
Adult Fat Body
11.6
Adult Salivary Gland
4.5
Adult Heart
50.475
Adult VirginFemale Spermatheca
8.5
Adult InseminatedFemale Spermatheca
9.4
Adult Ovary
8.3
Adult Testis
5.1
Adult Male Accessory Gland
8.8
Adult Carcass
15.7
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
26.025
Larval Midgut
13.7
Larval Hindgut
24.2
Larval Malpighian Tubules
42
Larval Fat Body
2.5
Larval Salivary Gland
3.1
Larval Trachea
47.7
Larval Carcass
32.375
Adult Head
21.1
Adult Eye
79.1
Adult Brain
8.2
Adult Thoracic-Abdominal Ganglion
10.5
Adult Crop
42.2
Adult Midgut
7.5
Adult Hindgut
18
Adult Malpighian Tubules
36.5
Adult Fat Body
11.6
Adult Salivary Gland
4.5
Adult Heart
50.475
Adult VirginFemale Spermatheca
8.5
Adult InseminatedFemale Spermatheca
9.4
Adult Ovary
8.3
Adult Testis
5.1
Adult Male Accessory Gland
8.8
Adult Carcass
15.7
Expression Level Scale
None
Low
Moderate
High
Very high
Heatmap
Tissue
Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass
FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level
Tissue
Adult Expression Level
NA Head
21.1
NA Eye
79.1
NA Brain
8.2
26.025 Central Nervous System
NA
NA Thoracic-Abdominal Ganglion
10.5
NA Crop
42.2
13.7 Midgut
7.5
24.2 Hindgut
18
42 Malpighian Tubules
36.5
2.5 Fat Body
11.6
3.1 Salivary Gland
4.5
NA Heart
50.475
47.7 Trachea
NA
NA VirginFemale Spermatheca
8.5
NA InseminatedFemale Spermatheca
9.4
NA Ovary
8.3
NA Testis
5.1
NA Male Accessory Gland
8.8
32.375 Carcass
no informative data
Linear, scaled to maximum expression level
Tissue
Expression Level
imaginal disc, larvae L3 wandering
6
central nervous system, larvae L3
2
central nervous system, pupae P8
4
head, virgin 1-day female
2
head, virgin 4-day female
3
head, virgin 20-day female
2
head, mated 1-day female
2
head, mated 4-day female
2
head, mated 20-day female
1
head, mated 1-day male
4
head, mated 4-day male
4
head, mated 20-day male
2
salivary gland, larvae L3 wandering
0
salivary gland, white prepupae
1
digestive system, larvae L3 wandering
1
digestive system, 1-day adult
1
digestive system, 4-day adult
1
digestive system, 20-day adult
1
fat body, larvae L3 wandering
0
fat body, white prepupae
1
fat body, pupae P8
1
carcass, larvae L3 wandering
4
carcass, 1-day adult
4
carcass, 4-day adult
3
carcass, 20-day adult
2
ovary, virgin 4-day female
1
ovary, mated 4-day female
0
testis, mated 4-day male
0
accessory gland, mated 4-day male
0
Expression Level Scale
Very low
Low
Moderate
Linear, scaled to Moderate expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
6
central nervous system, larvae L3
2
central nervous system, pupae P8
4
head, virgin 1-day female
2
head, virgin 4-day female
3
head, virgin 20-day female
2
head, mated 1-day female
2
head, mated 4-day female
2
head, mated 20-day female
1
head, mated 1-day male
4
head, mated 4-day male
4
head, mated 20-day male
2
salivary gland, larvae L3 wandering
0
salivary gland, white prepupae
1
digestive system, larvae L3 wandering
1
digestive system, 1-day adult
1
digestive system, 4-day adult
1
digestive system, 20-day adult
1
fat body, larvae L3 wandering
0
fat body, white prepupae
1
fat body, pupae P8
1
carcass, larvae L3 wandering
4
carcass, 1-day adult
4
carcass, 4-day adult
3
carcass, 20-day adult
2
ovary, virgin 4-day female
1
ovary, mated 4-day female
0
testis, mated 4-day male
0
accessory gland, mated 4-day male
0
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
6
central nervous system, larvae L3
2
central nervous system, pupae P8
4
head, virgin 1-day female
2
head, virgin 4-day female
3
head, virgin 20-day female
2
head, mated 1-day female
2
head, mated 4-day female
2
head, mated 20-day female
1
head, mated 1-day male
4
head, mated 4-day male
4
head, mated 20-day male
2
salivary gland, larvae L3 wandering
0
salivary gland, white prepupae
1
digestive system, larvae L3 wandering
1
digestive system, 1-day adult
1
digestive system, 4-day adult
1
digestive system, 20-day adult
1
fat body, larvae L3 wandering
0
fat body, white prepupae
1
fat body, pupae P8
1
carcass, larvae L3 wandering
4
carcass, 1-day adult
4
carcass, 4-day adult
3
carcass, 20-day adult
2
ovary, virgin 4-day female
1
ovary, mated 4-day female
0
testis, mated 4-day male
0
accessory gland, mated 4-day male
0
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
6
central nervous system, larvae L3
2
central nervous system, pupae P8
4
head, virgin 1-day female
2
head, virgin 4-day female
3
head, virgin 20-day female
2
head, mated 1-day female
2
head, mated 4-day female
2
head, mated 20-day female
1
head, mated 1-day male
4
head, mated 4-day male
4
head, mated 20-day male
2
salivary gland, larvae L3 wandering
0
salivary gland, white prepupae
1
digestive system, larvae L3 wandering
1
digestive system, 1-day adult
1
digestive system, 4-day adult
1
digestive system, 20-day adult
1
fat body, larvae L3 wandering
0
fat body, white prepupae
1
fat body, pupae P8
1
carcass, larvae L3 wandering
4
carcass, 1-day adult
4
carcass, 4-day adult
3
carcass, 20-day adult
2
ovary, virgin 4-day female
1
ovary, mated 4-day female
0
testis, mated 4-day male
0
accessory gland, mated 4-day male
0
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Tissue
Expression Level
imaginal disc, larvae L3 wandering
6
central nervous system, larvae L3
2
central nervous system, pupae P8
4
head, virgin 1-day female
2
head, virgin 4-day female
3
head, virgin 20-day female
2
head, mated 1-day female
2
head, mated 4-day female
2
head, mated 20-day female
1
head, mated 1-day male
4
head, mated 4-day male
4
head, mated 20-day male
2
salivary gland, larvae L3 wandering
0
salivary gland, white prepupae
1
digestive system, larvae L3 wandering
1
digestive system, 1-day adult
1
digestive system, 4-day adult
1
digestive system, 20-day adult
1
fat body, larvae L3 wandering
0
fat body, white prepupae
1
fat body, pupae P8
1
carcass, larvae L3 wandering
4
carcass, 1-day adult
4
carcass, 4-day adult
3
carcass, 20-day adult
2
ovary, virgin 4-day female
1
ovary, mated 4-day female
0
testis, mated 4-day male
0
accessory gland, mated 4-day male
0
Expression Level Scale
Very low
Low
Moderate
log, scaled to Moderate expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
6
central nervous system, larvae L3
2
central nervous system, pupae P8
4
head, virgin 1-day female
2
head, virgin 4-day female
3
head, virgin 20-day female
2
head, mated 1-day female
2
head, mated 4-day female
2
head, mated 20-day female
1
head, mated 1-day male
4
head, mated 4-day male
4
head, mated 20-day male
2
salivary gland, larvae L3 wandering
0
salivary gland, white prepupae
1
digestive system, larvae L3 wandering
1
digestive system, 1-day adult
1
digestive system, 4-day adult
1
digestive system, 20-day adult
1
fat body, larvae L3 wandering
0
fat body, white prepupae
1
fat body, pupae P8
1
carcass, larvae L3 wandering
4
carcass, 1-day adult
4
carcass, 4-day adult
3
carcass, 20-day adult
2
ovary, virgin 4-day female
1
ovary, mated 4-day female
0
testis, mated 4-day male
0
accessory gland, mated 4-day male
0
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
6
central nervous system, larvae L3
2
central nervous system, pupae P8
4
head, virgin 1-day female
2
head, virgin 4-day female
3
head, virgin 20-day female
2
head, mated 1-day female
2
head, mated 4-day female
2
head, mated 20-day female
1
head, mated 1-day male
4
head, mated 4-day male
4
head, mated 20-day male
2
salivary gland, larvae L3 wandering
0
salivary gland, white prepupae
1
digestive system, larvae L3 wandering
1
digestive system, 1-day adult
1
digestive system, 4-day adult
1
digestive system, 20-day adult
1
fat body, larvae L3 wandering
0
fat body, white prepupae
1
fat body, pupae P8
1
carcass, larvae L3 wandering
4
carcass, 1-day adult
4
carcass, 4-day adult
3
carcass, 20-day adult
2
ovary, virgin 4-day female
1
ovary, mated 4-day female
0
testis, mated 4-day male
0
accessory gland, mated 4-day male
0
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
6
central nervous system, larvae L3
2
central nervous system, pupae P8
4
head, virgin 1-day female
2
head, virgin 4-day female
3
head, virgin 20-day female
2
head, mated 1-day female
2
head, mated 4-day female
2
head, mated 20-day female
1
head, mated 1-day male
4
head, mated 4-day male
4
head, mated 20-day male
2
salivary gland, larvae L3 wandering
0
salivary gland, white prepupae
1
digestive system, larvae L3 wandering
1
digestive system, 1-day adult
1
digestive system, 4-day adult
1
digestive system, 20-day adult
1
fat body, larvae L3 wandering
0
fat body, white prepupae
1
fat body, pupae P8
1
carcass, larvae L3 wandering
4
carcass, 1-day adult
4
carcass, 4-day adult
3
carcass, 20-day adult
2
ovary, virgin 4-day female
1
ovary, mated 4-day female
0
testis, mated 4-day male
0
accessory gland, mated 4-day male
0
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Tissue
Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male
Linear, scaled to maximum expression level
Developmental Stage
Expression Level
embryo 00-02hr
0
embryo 02-04hr
3
embryo 04-06hr
11
embryo 06-08hr
10
embryo 08-10hr
8
embryo 10-12hr
4
embryo 12-14hr
6
embryo 14-16hr
7
embryo 16-18hr
9
embryo 18-20hr
6
embryo 20-22hr
2
embryo 22-24hr
2
larva L1
2
larva L2
2
larva L3 12hr old
2
larva L3 puffstage 1-2
1
larva L3 puffstage 3-6
3
larva L3 puffstage 7-9
3
white prepupae new
3
white prepupae 12hr
4
white prepupae 24hr
5
pupae 2d postWPP
5
pupae 3d postWPP
7
pupae 4d postWPP
4
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
2
adult female 05day
1
adult female 30day
1
Expression Level Scale
Very low
Low
Moderate
Linear, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
0
embryo 02-04hr
3
embryo 04-06hr
11
embryo 06-08hr
10
embryo 08-10hr
8
embryo 10-12hr
4
embryo 12-14hr
6
embryo 14-16hr
7
embryo 16-18hr
9
embryo 18-20hr
6
embryo 20-22hr
2
embryo 22-24hr
2
larva L1
2
larva L2
2
larva L3 12hr old
2
larva L3 puffstage 1-2
1
larva L3 puffstage 3-6
3
larva L3 puffstage 7-9
3
white prepupae new
3
white prepupae 12hr
4
white prepupae 24hr
5
pupae 2d postWPP
5
pupae 3d postWPP
7
pupae 4d postWPP
4
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
2
adult female 05day
1
adult female 30day
1
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
0
embryo 02-04hr
3
embryo 04-06hr
11
embryo 06-08hr
10
embryo 08-10hr
8
embryo 10-12hr
4
embryo 12-14hr
6
embryo 14-16hr
7
embryo 16-18hr
9
embryo 18-20hr
6
embryo 20-22hr
2
embryo 22-24hr
2
larva L1
2
larva L2
2
larva L3 12hr old
2
larva L3 puffstage 1-2
1
larva L3 puffstage 3-6
3
larva L3 puffstage 7-9
3
white prepupae new
3
white prepupae 12hr
4
white prepupae 24hr
5
pupae 2d postWPP
5
pupae 3d postWPP
7
pupae 4d postWPP
4
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
2
adult female 05day
1
adult female 30day
1
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
0
embryo 02-04hr
3
embryo 04-06hr
11
embryo 06-08hr
10
embryo 08-10hr
8
embryo 10-12hr
4
embryo 12-14hr
6
embryo 14-16hr
7
embryo 16-18hr
9
embryo 18-20hr
6
embryo 20-22hr
2
embryo 22-24hr
2
larva L1
2
larva L2
2
larva L3 12hr old
2
larva L3 puffstage 1-2
1
larva L3 puffstage 3-6
3
larva L3 puffstage 7-9
3
white prepupae new
3
white prepupae 12hr
4
white prepupae 24hr
5
pupae 2d postWPP
5
pupae 3d postWPP
7
pupae 4d postWPP
4
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
2
adult female 05day
1
adult female 30day
1
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Developmental Stage
Expression Level
embryo 00-02hr
0
embryo 02-04hr
3
embryo 04-06hr
11
embryo 06-08hr
10
embryo 08-10hr
8
embryo 10-12hr
4
embryo 12-14hr
6
embryo 14-16hr
7
embryo 16-18hr
9
embryo 18-20hr
6
embryo 20-22hr
2
embryo 22-24hr
2
larva L1
2
larva L2
2
larva L3 12hr old
2
larva L3 puffstage 1-2
1
larva L3 puffstage 3-6
3
larva L3 puffstage 7-9
3
white prepupae new
3
white prepupae 12hr
4
white prepupae 24hr
5
pupae 2d postWPP
5
pupae 3d postWPP
7
pupae 4d postWPP
4
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
2
adult female 05day
1
adult female 30day
1
Expression Level Scale
Very low
Low
Moderate
log, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
0
embryo 02-04hr
3
embryo 04-06hr
11
embryo 06-08hr
10
embryo 08-10hr
8
embryo 10-12hr
4
embryo 12-14hr
6
embryo 14-16hr
7
embryo 16-18hr
9
embryo 18-20hr
6
embryo 20-22hr
2
embryo 22-24hr
2
larva L1
2
larva L2
2
larva L3 12hr old
2
larva L3 puffstage 1-2
1
larva L3 puffstage 3-6
3
larva L3 puffstage 7-9
3
white prepupae new
3
white prepupae 12hr
4
white prepupae 24hr
5
pupae 2d postWPP
5
pupae 3d postWPP
7
pupae 4d postWPP
4
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
2
adult female 05day
1
adult female 30day
1
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
0
embryo 02-04hr
3
embryo 04-06hr
11
embryo 06-08hr
10
embryo 08-10hr
8
embryo 10-12hr
4
embryo 12-14hr
6
embryo 14-16hr
7
embryo 16-18hr
9
embryo 18-20hr
6
embryo 20-22hr
2
embryo 22-24hr
2
larva L1
2
larva L2
2
larva L3 12hr old
2
larva L3 puffstage 1-2
1
larva L3 puffstage 3-6
3
larva L3 puffstage 7-9
3
white prepupae new
3
white prepupae 12hr
4
white prepupae 24hr
5
pupae 2d postWPP
5
pupae 3d postWPP
7
pupae 4d postWPP
4
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
2
adult female 05day
1
adult female 30day
1
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
0
embryo 02-04hr
3
embryo 04-06hr
11
embryo 06-08hr
10
embryo 08-10hr
8
embryo 10-12hr
4
embryo 12-14hr
6
embryo 14-16hr
7
embryo 16-18hr
9
embryo 18-20hr
6
embryo 20-22hr
2
embryo 22-24hr
2
larva L1
2
larva L2
2
larva L3 12hr old
2
larva L3 puffstage 1-2
1
larva L3 puffstage 3-6
3
larva L3 puffstage 7-9
3
white prepupae new
3
white prepupae 12hr
4
white prepupae 24hr
5
pupae 2d postWPP
5
pupae 3d postWPP
7
pupae 4d postWPP
4
adult male 01day
3
adult male 05day
3
adult male 30day
3
adult female 01day
2
adult female 05day
1
adult female 30day
1
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Developmental Stage
Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day
Linear, scaled to maximum expression level
Cell Line
Expression Level
Schneider line 2 S2R+
0
Schneider line 2 Sg4
0
embryonic 1182-4H
3
embryonic GM2
2
embryonic Kc167
1
embryonic S1
0
embryonic S3
2
leg disc CME L1
0
wing disc CME-W2
1
wing disc ML-DmD8
0
wing disc ML-DmD9
3
wing disc ML-DmD16-c3
1
wing disc ML-DmD21
0
wing disc ML-DmD32
0
haltere disc ML-DmD17-c3
3
eye-antennal disc ML-DmD11
0
antennal disc ML-DmD20-c5
0
mixed discs ML-DmD4-c1
0
CNS ML-DmBG1-c1
1
CNS ML-DmBG2-c2
1
tumorous blood cells mbn2
1
ovary fGS/OSS
11
ovary OSC
8
ovary OSS
3
Expression Level Scale
Very low
Low
Moderate
Linear, scaled to Moderate expression
Cell Line
Expression Level
Schneider line 2 S2R+
0
Schneider line 2 Sg4
0
embryonic 1182-4H
3
embryonic GM2
2
embryonic Kc167
1
embryonic S1
0
embryonic S3
2
leg disc CME L1
0
wing disc CME-W2
1
wing disc ML-DmD8
0
wing disc ML-DmD9
3
wing disc ML-DmD16-c3
1
wing disc ML-DmD21
0
wing disc ML-DmD32
0
haltere disc ML-DmD17-c3
3
eye-antennal disc ML-DmD11
0
antennal disc ML-DmD20-c5
0
mixed discs ML-DmD4-c1
0
CNS ML-DmBG1-c1
1
CNS ML-DmBG2-c2
1
tumorous blood cells mbn2
1
ovary fGS/OSS
11
ovary OSC
8
ovary OSS
3
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Cell Line
Expression Level
Schneider line 2 S2R+
0
Schneider line 2 Sg4
0
embryonic 1182-4H
3
embryonic GM2
2
embryonic Kc167
1
embryonic S1
0
embryonic S3
2
leg disc CME L1
0
wing disc CME-W2
1
wing disc ML-DmD8
0
wing disc ML-DmD9
3
wing disc ML-DmD16-c3
1
wing disc ML-DmD21
0
wing disc ML-DmD32
0
haltere disc ML-DmD17-c3
3
eye-antennal disc ML-DmD11
0
antennal disc ML-DmD20-c5
0
mixed discs ML-DmD4-c1
0
CNS ML-DmBG1-c1
1
CNS ML-DmBG2-c2
1
tumorous blood cells mbn2
1
ovary fGS/OSS
11
ovary OSC
8
ovary OSS
3
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Cell Line
Expression Level
Schneider line 2 S2R+
0
Schneider line 2 Sg4
0
embryonic 1182-4H
3
embryonic GM2
2
embryonic Kc167
1
embryonic S1
0
embryonic S3
2
leg disc CME L1
0
wing disc CME-W2
1
wing disc ML-DmD8
0
wing disc ML-DmD9
3
wing disc ML-DmD16-c3
1
wing disc ML-DmD21
0
wing disc ML-DmD32
0
haltere disc ML-DmD17-c3
3
eye-antennal disc ML-DmD11
0
antennal disc ML-DmD20-c5
0
mixed discs ML-DmD4-c1
0
CNS ML-DmBG1-c1
1
CNS ML-DmBG2-c2
1
tumorous blood cells mbn2
1
ovary fGS/OSS
11
ovary OSC
8
ovary OSS
3
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Cell Line
Expression Level
Schneider line 2 S2R+
0
Schneider line 2 Sg4
0
embryonic 1182-4H
3
embryonic GM2
2
embryonic Kc167
1
embryonic S1
0
embryonic S3
2
leg disc CME L1
0
wing disc CME-W2
1
wing disc ML-DmD8
0
wing disc ML-DmD9
3
wing disc ML-DmD16-c3
1
wing disc ML-DmD21
0
wing disc ML-DmD32
0
haltere disc ML-DmD17-c3
3
eye-antennal disc ML-DmD11
0
antennal disc ML-DmD20-c5
0
mixed discs ML-DmD4-c1
0
CNS ML-DmBG1-c1
1
CNS ML-DmBG2-c2
1
tumorous blood cells mbn2
1
ovary fGS/OSS
11
ovary OSC
8
ovary OSS
3
Expression Level Scale
Very low
Low
Moderate
log, scaled to Moderate expression
Cell Line
Expression Level
Schneider line 2 S2R+
0
Schneider line 2 Sg4
0
embryonic 1182-4H
3
embryonic GM2
2
embryonic Kc167
1
embryonic S1
0
embryonic S3
2
leg disc CME L1
0
wing disc CME-W2
1
wing disc ML-DmD8
0
wing disc ML-DmD9
3
wing disc ML-DmD16-c3
1
wing disc ML-DmD21
0
wing disc ML-DmD32
0
haltere disc ML-DmD17-c3
3
eye-antennal disc ML-DmD11
0
antennal disc ML-DmD20-c5
0
mixed discs ML-DmD4-c1
0
CNS ML-DmBG1-c1
1
CNS ML-DmBG2-c2
1
tumorous blood cells mbn2
1
ovary fGS/OSS
11
ovary OSC
8
ovary OSS
3
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Cell Line
Expression Level
Schneider line 2 S2R+
0
Schneider line 2 Sg4
0
embryonic 1182-4H
3
embryonic GM2
2
embryonic Kc167
1
embryonic S1
0
embryonic S3
2
leg disc CME L1
0
wing disc CME-W2
1
wing disc ML-DmD8
0
wing disc ML-DmD9
3
wing disc ML-DmD16-c3
1
wing disc ML-DmD21
0
wing disc ML-DmD32
0
haltere disc ML-DmD17-c3
3
eye-antennal disc ML-DmD11
0
antennal disc ML-DmD20-c5
0
mixed discs ML-DmD4-c1
0
CNS ML-DmBG1-c1
1
CNS ML-DmBG2-c2
1
tumorous blood cells mbn2
1
ovary fGS/OSS
11
ovary OSC
8
ovary OSS
3
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Cell Line
Expression Level
Schneider line 2 S2R+
0
Schneider line 2 Sg4
0
embryonic 1182-4H
3
embryonic GM2
2
embryonic Kc167
1
embryonic S1
0
embryonic S3
2
leg disc CME L1
0
wing disc CME-W2
1
wing disc ML-DmD8
0
wing disc ML-DmD9
3
wing disc ML-DmD16-c3
1
wing disc ML-DmD21
0
wing disc ML-DmD32
0
haltere disc ML-DmD17-c3
3
eye-antennal disc ML-DmD11
0
antennal disc ML-DmD20-c5
0
mixed discs ML-DmD4-c1
0
CNS ML-DmBG1-c1
1
CNS ML-DmBG2-c2
1
tumorous blood cells mbn2
1
ovary fGS/OSS
11
ovary OSC
8
ovary OSS
3
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Cell Line
Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS
Linear, scaled to maximum expression level
Treatment
Expression Level
extended cold, 4-day adult
1
cold shock, 4-day adult
1
heat shock, 4-day adult
1
Cadmium 50 mM 6 hrs, larvae L3
1
Cadmium 50 mM 12 hrs, larvae L3
1
Cadmium 50 mM 48 hrs, 4-day adult
1
Cadmium 100 mM 48 hrs, 4-day adult
2
Copper 0.5 mM 12 hrs, larvae L3
1
Copper 15 mM 48 hrs, 4-day adult
2
Zinc 5 mM 12 hrs, larvae L3
1
Zinc 4.5 mM 48 hrs, 4-day adult
3
Ethanol 2.5% 3 hrs, larvae L3
1
Ethanol 5% 3 hrs, larvae L3
1
Ethanol 10% 3 hrs, larvae L3
1
Caffeine 1.5 mg/ml 4 hrs, larvae L3
1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
1
Caffeine 25 mg/ml 48 hrs, 4-day adult
1
Paraquat 5 mM 48 hrs, 4-day adult
1
Paraquat 10 mM 48 hrs, 4-day adult
1
Rotenone 2 μg 12 hrs, larvae L3
1
Rotenone 8 μg 12 hrs, larvae L3
1
Expression Level Scale
Very low
Low
Moderate
Linear, scaled to Moderate expression
Treatment
Expression Level
extended cold, 4-day adult
1
cold shock, 4-day adult
1
heat shock, 4-day adult
1
Cadmium 50 mM 6 hrs, larvae L3
1
Cadmium 50 mM 12 hrs, larvae L3
1
Cadmium 50 mM 48 hrs, 4-day adult
1
Cadmium 100 mM 48 hrs, 4-day adult
2
Copper 0.5 mM 12 hrs, larvae L3
1
Copper 15 mM 48 hrs, 4-day adult
2
Zinc 5 mM 12 hrs, larvae L3
1
Zinc 4.5 mM 48 hrs, 4-day adult
3
Ethanol 2.5% 3 hrs, larvae L3
1
Ethanol 5% 3 hrs, larvae L3
1
Ethanol 10% 3 hrs, larvae L3
1
Caffeine 1.5 mg/ml 4 hrs, larvae L3
1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
1
Caffeine 25 mg/ml 48 hrs, 4-day adult
1
Paraquat 5 mM 48 hrs, 4-day adult
1
Paraquat 10 mM 48 hrs, 4-day adult
1
Rotenone 2 μg 12 hrs, larvae L3
1
Rotenone 8 μg 12 hrs, larvae L3
1
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Treatment
Expression Level
extended cold, 4-day adult
1
cold shock, 4-day adult
1
heat shock, 4-day adult
1
Cadmium 50 mM 6 hrs, larvae L3
1
Cadmium 50 mM 12 hrs, larvae L3
1
Cadmium 50 mM 48 hrs, 4-day adult
1
Cadmium 100 mM 48 hrs, 4-day adult
2
Copper 0.5 mM 12 hrs, larvae L3
1
Copper 15 mM 48 hrs, 4-day adult
2
Zinc 5 mM 12 hrs, larvae L3
1
Zinc 4.5 mM 48 hrs, 4-day adult
3
Ethanol 2.5% 3 hrs, larvae L3
1
Ethanol 5% 3 hrs, larvae L3
1
Ethanol 10% 3 hrs, larvae L3
1
Caffeine 1.5 mg/ml 4 hrs, larvae L3
1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
1
Caffeine 25 mg/ml 48 hrs, 4-day adult
1
Paraquat 5 mM 48 hrs, 4-day adult
1
Paraquat 10 mM 48 hrs, 4-day adult
1
Rotenone 2 μg 12 hrs, larvae L3
1
Rotenone 8 μg 12 hrs, larvae L3
1
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Treatment
Expression Level
extended cold, 4-day adult
1
cold shock, 4-day adult
1
heat shock, 4-day adult
1
Cadmium 50 mM 6 hrs, larvae L3
1
Cadmium 50 mM 12 hrs, larvae L3
1
Cadmium 50 mM 48 hrs, 4-day adult
1
Cadmium 100 mM 48 hrs, 4-day adult
2
Copper 0.5 mM 12 hrs, larvae L3
1
Copper 15 mM 48 hrs, 4-day adult
2
Zinc 5 mM 12 hrs, larvae L3
1
Zinc 4.5 mM 48 hrs, 4-day adult
3
Ethanol 2.5% 3 hrs, larvae L3
1
Ethanol 5% 3 hrs, larvae L3
1
Ethanol 10% 3 hrs, larvae L3
1
Caffeine 1.5 mg/ml 4 hrs, larvae L3
1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
1
Caffeine 25 mg/ml 48 hrs, 4-day adult
1
Paraquat 5 mM 48 hrs, 4-day adult
1
Paraquat 10 mM 48 hrs, 4-day adult
1
Rotenone 2 μg 12 hrs, larvae L3
1
Rotenone 8 μg 12 hrs, larvae L3
1
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Treatment
Expression Level
extended cold, 4-day adult
1
cold shock, 4-day adult
1
heat shock, 4-day adult
1
Cadmium 50 mM 6 hrs, larvae L3
1
Cadmium 50 mM 12 hrs, larvae L3
1
Cadmium 50 mM 48 hrs, 4-day adult
1
Cadmium 100 mM 48 hrs, 4-day adult
2
Copper 0.5 mM 12 hrs, larvae L3
1
Copper 15 mM 48 hrs, 4-day adult
2
Zinc 5 mM 12 hrs, larvae L3
1
Zinc 4.5 mM 48 hrs, 4-day adult
3
Ethanol 2.5% 3 hrs, larvae L3
1
Ethanol 5% 3 hrs, larvae L3
1
Ethanol 10% 3 hrs, larvae L3
1
Caffeine 1.5 mg/ml 4 hrs, larvae L3
1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
1
Caffeine 25 mg/ml 48 hrs, 4-day adult
1
Paraquat 5 mM 48 hrs, 4-day adult
1
Paraquat 10 mM 48 hrs, 4-day adult
1
Rotenone 2 μg 12 hrs, larvae L3
1
Rotenone 8 μg 12 hrs, larvae L3
1
Expression Level Scale
Very low
Low
Moderate
log, scaled to Moderate expression
Treatment
Expression Level
extended cold, 4-day adult
1
cold shock, 4-day adult
1
heat shock, 4-day adult
1
Cadmium 50 mM 6 hrs, larvae L3
1
Cadmium 50 mM 12 hrs, larvae L3
1
Cadmium 50 mM 48 hrs, 4-day adult
1
Cadmium 100 mM 48 hrs, 4-day adult
2
Copper 0.5 mM 12 hrs, larvae L3
1
Copper 15 mM 48 hrs, 4-day adult
2
Zinc 5 mM 12 hrs, larvae L3
1
Zinc 4.5 mM 48 hrs, 4-day adult
3
Ethanol 2.5% 3 hrs, larvae L3
1
Ethanol 5% 3 hrs, larvae L3
1
Ethanol 10% 3 hrs, larvae L3
1
Caffeine 1.5 mg/ml 4 hrs, larvae L3
1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
1
Caffeine 25 mg/ml 48 hrs, 4-day adult
1
Paraquat 5 mM 48 hrs, 4-day adult
1
Paraquat 10 mM 48 hrs, 4-day adult
1
Rotenone 2 μg 12 hrs, larvae L3
1
Rotenone 8 μg 12 hrs, larvae L3
1
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Treatment
Expression Level
extended cold, 4-day adult
1
cold shock, 4-day adult
1
heat shock, 4-day adult
1
Cadmium 50 mM 6 hrs, larvae L3
1
Cadmium 50 mM 12 hrs, larvae L3
1
Cadmium 50 mM 48 hrs, 4-day adult
1
Cadmium 100 mM 48 hrs, 4-day adult
2
Copper 0.5 mM 12 hrs, larvae L3
1
Copper 15 mM 48 hrs, 4-day adult
2
Zinc 5 mM 12 hrs, larvae L3
1
Zinc 4.5 mM 48 hrs, 4-day adult
3
Ethanol 2.5% 3 hrs, larvae L3
1
Ethanol 5% 3 hrs, larvae L3
1
Ethanol 10% 3 hrs, larvae L3
1
Caffeine 1.5 mg/ml 4 hrs, larvae L3
1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
1
Caffeine 25 mg/ml 48 hrs, 4-day adult
1
Paraquat 5 mM 48 hrs, 4-day adult
1
Paraquat 10 mM 48 hrs, 4-day adult
1
Rotenone 2 μg 12 hrs, larvae L3
1
Rotenone 8 μg 12 hrs, larvae L3
1
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Treatment
Expression Level
extended cold, 4-day adult
1
cold shock, 4-day adult
1
heat shock, 4-day adult
1
Cadmium 50 mM 6 hrs, larvae L3
1
Cadmium 50 mM 12 hrs, larvae L3
1
Cadmium 50 mM 48 hrs, 4-day adult
1
Cadmium 100 mM 48 hrs, 4-day adult
2
Copper 0.5 mM 12 hrs, larvae L3
1
Copper 15 mM 48 hrs, 4-day adult
2
Zinc 5 mM 12 hrs, larvae L3
1
Zinc 4.5 mM 48 hrs, 4-day adult
3
Ethanol 2.5% 3 hrs, larvae L3
1
Ethanol 5% 3 hrs, larvae L3
1
Ethanol 10% 3 hrs, larvae L3
1
Caffeine 1.5 mg/ml 4 hrs, larvae L3
1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
1
Caffeine 25 mg/ml 48 hrs, 4-day adult
1
Paraquat 5 mM 48 hrs, 4-day adult
1
Paraquat 10 mM 48 hrs, 4-day adult
1
Rotenone 2 μg 12 hrs, larvae L3
1
Rotenone 8 μg 12 hrs, larvae L3
1
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Treatment
Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3
A cluster of genes with similar mRNA expression dynamics across development.
Linkouts
- Cell culture data for RNAi and other high-throughput technologies
- Adult expression by tissue, using Affymetrix Dros2 array
adult cuticle & head capsule | dorsal
dorsal mesothoracic disc & peripodial epithelium | somatic clone | cell non-autonomous, with
Scer\GAL4αTub84B.PL sensory neuron & axon & embryo
For All Classical Alleles Show For All Alleles Carried on Transgenic
Constructs Show Disrupted in Duplicated in Transgenic Constructs UAS construct
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
characterization construct
Insertions insertion of enhancer trap
insertion of enhancer trap binary system
insertion of mobile activating element
Molecular Function inferred from mutant phenotype
(assigned by UniProtKB)
inferred from direct assay
Biological Process inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProtKB)
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
Cellular Component ( 0 terms) Molecular Function non-traceable author statement
traceable author statement
Biological Process traceable author statement
non-traceable author statement
traceable author statement
traceable author statement
traceable author statement
traceable author statement
traceable author statement
non-traceable author statement
Cellular Component non-traceable author statement
non-traceable author statement
(assigned by UniProtKB)
traceable author statement
non-traceable author statement
Interacts with Please look at the allele data
for full details of the genetic interactions
Linkouts
- A database of protein and genetic interactions
- A comprehensive database of gene and protein interactions.
Protein-protein interactions (PPI) from both known and predicted PPI data sets.
OrthoDB Ortholog Groups
OrthoDB orthology group searches
Drosophila inclusive ortholog search
Dipteran inclusive ortholog search
Insect inclusive ortholog search
Arthropod inclusive ortholog search
Metazoa inclusive ortholog search
No orthologs identified
EOG6DFQ78 )
Drosophila melanogaster
fruit fly
Drosophila pseudoobscura pseudoobscura
Y
EOG6PCB2G )
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
EOG6W3RHP )
Apis mellifera
Western honey bee
Amel\GB51048
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG026967
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG020095
Acromyrmex echinatior
Panamanian leafcutter ant
Aech\AECH24894
Acromyrmex echinatior
Panamanian leafcutter ant
Aech\AECH24897
Atta cephalotes
Leafcutter ant
Acep\ACEP12457
Camponotus floridanus
Florida carpenter ant
Cflo\CFLO16519
Harpegnathos saltator
Jerdons jumping ant
Hsal\HSAL12172
Harpegnathos saltator
Jerdons jumping ant
Hsal\HSAL14128
Harpegnathos saltator
Jerdons jumping ant
Hsal\HSAL14129
Linepithema humile
Argentine ant
Lhum\LH13695
Linepithema humile
Argentine ant
Lhum\LH13696
Pogonomyrmex barbatus
Red harvester ant
Pbar\PB13961
Solenopsis invicta
Red fire ant
Sinv\SINV24980
Acyrthosiphon pisum
Pea aphid
Pediculus humanus
Human body louse
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
EOG69S4Q1 )
Ixodes scapularis
Deer tick
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
AAA Orthologs (11)
based on analysis using Dmel annotation version 4.3
Drosophila pseudoobscura pseudoobscura
Bloomington Harvard Kyoto VDRC Please Note FlyBase no
longer curates genomic clone accessions so this list
may not be complete
Please Note This section lists
cDNAs and ESTs that fall within the genomic extent
of the gene model, which may include cDNAs and ESTs
of genes within introns, or of overlapping genes.
Please see
GBrowse for alignment of the cDNAs and ESTs
to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones Other clones cDNA Clones, End Sequenced
(ESTs) BDGP DGC clones Other clones Linkouts
- Results from RNAi screens.
- GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
Source for database identity of Source for identity of: vn CG10491
Source for database merge of Additional comments Map position and phenotype suggest of "vn1 " suggests a possible relationship to "Vn1 ", but allelism has not been tested as "Vn1 " is no longer available.
Six alleles (Diaz-Benjumea et al., 1989). Not tested for allelism with Vn, a dominant mutant of similar map position and phenotype which has been lost.
rho ,
sim and
vn are required for the formation of the brain lateral to the foregut cells.
The
vn /
Egfr pathway may be directly required for development of the notum by activating notum specifying genes and indirectly controlling wing outgrowth through regulation of
ap .
hh activates
vn expression. This activation is mediated through the gene
ci .
vn is required for the development of some, but not all, of the
hh -dependent medial head structures.
vn has a minor role in the embryo and does not induce
Egfr target genes such as
argos and
pnt in the embryo.
vn has a major role in wing development and
vn /
Egfr signal is a potent inducer of
Egfr target genes in the wing disc.
EGF domain swapping experiments of
vn ,
spi and
argos demonstrate that the EGF domain is the key determinant that gives
Egfr inhibitors and activators their distinct properties.
vn is the activating ligand of
Egfr in the midgut.
vn plays a permissive role in the induction of the endoderm by
dpp and
wg , which in turn up-regulate
vn expression in the midgut mesoderm in two regions overlapping the
dpp sources.
vn plays a role in autocrine
Egfr signaling.
vn is required to complement
spi in the development of a subset of muscle precursors whose differentiation depends on
Egfr activity.
Genetic combinations with mutants of
nub cause additive phenotypes.
A screen to isolate mutations that cause dominant enhancement of wing vein phenotypes associated with
Egfr recovered mutations in
vn .
In vivo culture of mutant discs from genotypes that are normally embryonic lethal demonstrates
vn is essential for wing disc growth.
The molecular signal for terminal differentiation of tendon cells is secreted
vn protein produced by the myotubes.
Loss of function mutations in
bs are epistatic to loss of function mutations in
rho or
vn .
Molecular and genetic data suggest that
vn is linked to the
Egfr pathway and encodes a ligand for the
Egfr product.
Phenotype and expression analysis show
vn has an early role in global proliferation of the wing disc and specific roles in the development of the notum, hinge, longitudinal vein 4 and all intervein regions.
Mutant embryos have a phenotype similar to that seen in
sr , with abnormal direction of myotubule filopodia.
Phenotypic analysis suggests
vn is a new member of the
spi group.
Meiotic location, larval phenotype, clonal behaviour, complementation tests and
vn heteroallelic combinations suggest
vn and
vn are allelic.
Genetic mosaics demonstrate that cell proliferation is reduced in all regions of the wing blade in a local autonomous way, the effects are more extreme when clones occupy intervein regions bordering veins. Clones also have nonautonomous effects in the proliferation of wild type cells in the same wing, these effects are more extreme in double mutants with
rho .
Mutations in vn cause pleiotropic phenotypes in embryonic patterns and affect several longitudinal veins.
ve, vn, ci, cg, svs, ast, H, Vno and vvl belong to the vein phenotypic group (Puro, 1982, Droso. Info. Serv. 58:205--208) within the 'lack-of-vein' mutant class. Loss-of-function alleles at these loci remove stretches of veins in two or more longitudinal veins. Double mutations within members of this group remove all veins, have smaller, slightly lanceolate wings, no sensilla and extra chaetae. Some alleles are embryonic lethal.
Homozygous ddd larvae perish between the first larval instar and the prepupal stage; dorsal thoracic imaginal discs, i.e. of the pronotum, mesonotum and metanotum reduced to 3% or less of normal size; all other imaginal discs develop normally. Mutant larvae support the growth of wild-type wing discs; mutant wing discs show very little development in wild-type larval. Mutant cells develop normally in wing discs that contain mixtures of mutant and wild-type cells, as produced by nuclear or cellular transplantation into blastoderms or by somatic exchange. Mutant leg discs transplanted into wild-type hosts can transdetermine to wing development. Studies of temperature-sensitive genotypes indicate that
vn + product is not required for normal wing development during embryogenesis. No evidence for a maternal effect in either conditional mutants raised under permissive conditions and switched to restrictive temperatures or in germ-line-transplants of mutant cells into wild-type hosts.
Sequence Crossreferences
- A searchable database of RefSeq genes.
Other Crossreferences
domains - A database of protein families, domains, and functional sites
Linkouts
- A database of protein and genetic interactions
- A comprehensive database of gene and protein interactions.
- Results from RNAi screens.
- Cell culture data for RNAi and other high-throughput technologies
- Adult expression by tissue, using Affymetrix Dros2 array
- Integrated genomics database for Drosophila, Anopheles, and C.elegans
- GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
- A cyberspace guide to Drosophila development and metazoan evolution
Protein-protein interactions (PPI) from both known and predicted PPI data sets.
- Data generated by the modENCODE project.
- A database of transcriptional regulatory elements.
Reported As Symbol Synonym vn
(
Ramos and Mohler, 2006 ,
Parker, 2006 ,
Zinzen et al., 2006 ,
Charroux et al., 2006 ,
Zeitlinger et al., 2007 ,
Sandmann et al., 2007 ,
Stathopoulos and Levine, 2005 ,
Galindo et al., 2005 ,
Butchar et al., 2012 ,
Christensen et al., 2008.4.15 ,
Christensen et al., 2008.4.15 ,
Christensen et al., 2008.4.15 ,
Macdonald and Long, 2005 ,
Markstein et al., 2004 ,
Slattery et al., 2011 ,
Liu and Geisbrecht, 2011 ,
Takemura and Adachi-Yamada, 2011 ,
Moses et al., 2011 ,
Hwang and Rulifson, 2011 ,
Choksi et al., 2006 ,
Poernbacher et al., 2012 ,
Rafel and Milán, 2008 ,
Huh et al., 2009 ,
Angulo et al., 2004 ,
Schlesinger et al., 2004 ,
Terriente-Félix and de Celis, 2009 ,
Muse et al., 2007 ,
Oishi et al., 2006 ,
Oishi et al., 2009 ,
Grieder et al., 2009 ,
Zhang et al., 2009 ,
Klein et al., 2010 ,
Guichard et al., 2006 ,
Foley et al., 2010 ,
Rendina et al., 2010 ,
Chang et al., 2001 ,
Bosch et al., 2010 ,
Beltran et al., 2007 ,
Jiang and Edgar, 2009 ,
Ajuria et al., 2011 ,
Hogan et al., 2011 ,
Jiang et al., 2011 ,
Wang et al., 2011 ,
Ozdemir et al., 2011 ,
Murillo-Maldonado et al., 2011 ,
Japanese National Institute of Genetics, 2012.5.21 ,
Sinenko et al., 2011 ,
Chang et al., 2003 ,
Maeng et al., 2012 ,
Chakrabarti et al., 2012 )
Name Synonym vein
(
Pallavi et al., 2006 ,
Galindo et al., 2005 ,
Letizia et al., 2007 ,
Takanaka and Courey, 2005 ,
Markstein et al., 2004 ,
Turner et al., 2011 ,
Takemura and Adachi-Yamada, 2011 ,
Moses et al., 2011 ,
Biteau and Jasper, 2011 ,
Rafel and Milán, 2008 ,
Foronda et al., 2009 ,
Santos et al., 2005 ,
Oishi et al., 2006 ,
Lawlor et al., 2011 ,
Zhang et al., 2009 ,
Klein et al., 2010 ,
Jiang and Edgar, 2009 ,
Buchon et al., 2010 ,
Ajuria et al., 2011 ,
Jiang et al., 2011 ,
Wang et al., 2011 ,
Ozdemir et al., 2011 ,
Kim et al., 2008 ,
Maeng et al., 2012 )
Vein
(
McDonald et al., 2006 ,
Inaki et al., 2012 ,
Liu and Geisbrecht, 2011 ,
Buchon et al., 2009 ,
Ren et al., 2010 ,
Murillo-Maldonado et al., 2011 )
Secondary FlyBase
IDs FBgn0001609 FBgn0002436 FBgn0010946 FBgn0011493 Generate a list of List References by type
EGFR signaling in the brain is necessary for olfactory learning in Drosophila larvae. [FBrf0221122] New negative feedback regulators of egfr signaling in Drosophila. [FBrf0219175] Infection-induced host translational blockage inhibits immune responses and epithelial renewal in the Drosophila gut. [FBrf0218984] Effective guidance of collective migration based on differences in cell states. [FBrf0217439] The BTB/POZ-ZF transcription factor dPLZF is involved in Ras/ERK signaling during Drosophila wing development. [FBrf0218378] Drosophila Pez acts in hippo signaling to restrict intestinal stem cell proliferation. [FBrf0217766] Capicua DNA-binding sites are general response elements for RTK signaling in Drosophila. [FBrf0212975] GlycoFly: A Database of Drosophila N-linked Glycoproteins Identified Using SPEG-MS Techniques. [FBrf0213834] EGF signaling regulates the proliferation of intestinal stem cells in Drosophila. [FBrf0213057] A Novel Interaction Between hedgehog and Notch Promotes Proliferation at the Anterior-Posterior Organizer of the Drosophila Wing. [FBrf0212986] A targeted genetic screen identifies crucial players in the specification of the Drosophila abdominal Capaergic neurons. [FBrf0213290] Two frizzled planar cell polarity signals in the Drosophila wing are differentially organized by the fat/dachsous pathway. [FBrf0213159] Serial specification of diverse neuroblast identities from a neurogenic placode by Notch and Egfr signaling. [FBrf0214015] EGFR/Ras/MAPK Signaling Mediates Adult Midgut Epithelial Homeostasis and Regeneration in Drosophila. [FBrf0212694] Double-stranded RNA is pathogenic in Drosophila models of expanded repeat neurodegenerative diseases. [FBrf0215240] Moleskin is essential for the formation of the myotendinous junction in Drosophila. [FBrf0216515] Phosphorylation of Ind by MAP kinase enhances Ind-dependent transcriptional repression. [FBrf0216621] Insulin Receptor-Mediated Signaling via Phospholipase C-γ Regulates Growth and Differentiation in Drosophila. [FBrf0216849] High resolution mapping of Twist to DNA in Drosophila embryos: Efficient functional analysis and evolutionary conservation. [FBrf0213359] Oxidative stress in the haematopoietic niche regulates the cellular immune response in Drosophila. [FBrf0217071] Genome-wide tissue-specific occupancy of the hox protein ultrabithorax and hox cofactor homothorax in Drosophila. [FBrf0213426] Cell death and selective adhesion reorganize the dorsoventral boundary for zigzag patterning of Drosophila wing margin hairs. [FBrf0214791] Population-Based Resequencing of Experimentally Evolved Populations Reveals the Genetic Basis of Body Size Variation in Drosophila melanogaster. [FBrf0213327] Sexually dimorphic regulation of the Wingless morphogen controls sex-specific segment number in Drosophila. [FBrf0214239] EGFR, Wingless and JAK/STAT signaling cooperatively maintain Drosophila intestinal stem cells. [FBrf0213634]
All reviews listed in FlyBase were published before 2011 
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