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General Information
Symbol
Dmel\Z600
Species
D. melanogaster
Name
Z600
Annotation Symbol
CG17962
Feature Type
FlyBase ID
FBgn0004052
Gene Model Status
Stock Availability
Gene Snapshot
Z600 (Z600) encodes a mitotic inhibitor that binds to the hydrophobic patch of Cyclins. It contributes to the switch in cell cycle mode in blastoderm embryos, and delaying mitosis in the mesoderm anlage to avoid an interference of mitosis and morphogenesis. [Date last reviewed: 2019-03-21]
Also Known As
frs, fruhstart, DMZ60MEX
Key Links
Genomic Location
Cytogenetic map
Sequence location
3L:15,509,061..15,509,513 [+]
Recombination map
3-43
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Molecular Function (GO)
    [Detailed GO annotations]
    Experimental Evidence
    Predictions / Assertions
    -
    Summaries
    Protein Function (UniProtKB)
    Cell cycle regulator that is involved in modulating and adjusting cell proliferation according to the requirements of the developmental program (PubMed:10850494, PubMed:12919679, PubMed:17431409). Interacts with mitotic Cdk1-cyclin complexes to inhibit mitotic entry at the G2/M transition (PubMed:17431409). Likely to function by binding to the hydrophobic patch of cyclins to interfere with the interaction between the complex and certain Cdk1 substrates (PubMed:17431409). At the mid-blastula transition, involved in the cell cycle arrest in G2 of cycle 14 by delaying mitosis and thus reducing cell poliferation allowing cell fate specification and morphogenesis to take place (PubMed:12919679). Acts downstream or in parallel to the checkpoint regulator grp which is also required for the cell cycle pause at cycle 14 (PubMed:12919679). During gastrulation, delays mitosis in the ventral region of the embryonic mesoderm thus allowing invagination to be completed before cell division takes place (PubMed:10850494, PubMed:12919679, PubMed:17431409).
    (UniProt, P22469)
    Phenotypic Description (Red Book; Lindsley and Zimm 1992)
    Z600
    Member of cluster of genes that includes Eip28/29 and Gdl. Believed to play role in processes of early development.
    Gene Model and Products
    Number of Transcripts
    1
    Number of Unique Polypeptides
    1

    Please see the GBrowse view of Dmel\Z600 or the JBrowse view of Dmel\Z600 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model
    Gene model reviewed during 5.44
    Gene model reviewed during 5.46
    Sequence Ontology: Class of Gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    FBtr0075549
    453
    90
    Additional Transcript Data and Comments
    Reported size (kB)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    FBpp0075304
    10.5
    90
    10.59
    Polypeptides with Identical Sequences

    There is only one protein coding transcript and one polypeptide associated with this gene

    Additional Polypeptide Data and Comments
    Reported size (kDa)
    18 (kD observed); 10.5 (kD predicted)
    Comments
    External Data
    Subunit Structure (UniProtKB)
    Component of the Frs-CycA-Cdk1 complex composed of Z600, CycA and Cdk1. Interacts preferentially with CycA (via C-terminus) but is also able to interact (via C-terminus) with CycE (via C-terminus).
    (UniProt, P22469)
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Z600 using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Gene Ontology (7 terms)
    Molecular Function (2 terms)
    Terms Based on Experimental Evidence (2 terms)
    CV Term
    Evidence
    References
    inferred from physical interaction with FLYBASE:CycA; FB:FBgn0000404
    inferred from physical interaction with FLYBASE:CycE; FB:FBgn0010382
    inferred from physical interaction with FLYBASE:Nup50; FB:FBgn0033264
    Terms Based on Predictions or Assertions (0 terms)
    Biological Process (4 terms)
    Terms Based on Experimental Evidence (3 terms)
    CV Term
    Evidence
    References
    Terms Based on Predictions or Assertions (2 terms)
    CV Term
    Evidence
    References
    Cellular Component (1 term)
    Terms Based on Experimental Evidence (1 term)
    CV Term
    Evidence
    References
    inferred from direct assay
    Terms Based on Predictions or Assertions (1 term)
    CV Term
    Evidence
    References
    non-traceable author statement
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    in situ
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    northern blot
    Stage
    Tissue/Position (including subcellular localization)
    Reference

    Comment: reference states 0-4 hr AEL

    organism | dorsal

    Comment: late embryonic stage 4

    organism | posterior

    Comment: late embryonic stage 4

    Additional Descriptive Data
    Z600 transcripts are detected starting at embryonic cycle 13. Transcripts initially accumulate uniformly, but become concentrated in the dorsal and posterior regions during cellularization, and persist in the dorsal regions of the embryo during gastrulation and germ band extension. No signal is detected after germ band extension.
    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    western blot
    Stage
    Tissue/Position (including subcellular localization)
    Reference

    Comment: reference states 0-4 hr AEL

    Additional Descriptive Data
    The 18 kD Z600 protein is detected at low levels in 0-2 hr embryos and at higher levels in 2-4 hr embryos. No expression is detected in older embryos or in unfertilized eggs. Immunolocalization experiments reveal that expression is found only in nuclear cycle 11-13 embryos, associated with condensed chromatin. Protein is not detected at later stages, despite the presence of Z600 transcript.
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    inferred from direct assay
    Expression Deduced from Reporters
    Reporter: P{z600-lacZ}
    Stage
    Tissue/Position (including subcellular localization)
    Reference

    Comment: reference states 0-6 hr AEL

    organism | dorsal

    Comment: late embryonic stage 4

    organism | posterior

    Comment: late embryonic stage 4

    organism | ventral

    Comment: late embryonic stage 4

    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\Z600 in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Flygut - An atlas of the Drosophila adult midgut
    Images
    Alleles, Insertions, and Transgenic Constructs
    Classical and Insertion Alleles ( 1 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 12 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of Z600
    Transgenic constructs containing regulatory region of Z600
    Deletions and Duplications ( 9 )
    Phenotypes
    Orthologs
    Human Orthologs (via DIOPT v7.1)
    Homo sapiens (Human) (0)
    No records found.
    Model Organism Orthologs (via DIOPT v7.1)
    Mus musculus (laboratory mouse) (0)
    No records found.
    Rattus norvegicus (Norway rat) (0)
    No records found.
    Xenopus tropicalis (Western clawed frog) (0)
    No records found.
    Danio rerio (Zebrafish) (0)
    No records found.
    Caenorhabditis elegans (Nematode, roundworm) (0)
    No records found.
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190K3C )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila melanogaster
    fruit fly
    Drosophila suzukii
    Spotted wing Drosophila
    Drosophila simulans
    Drosophila sechellia
    Drosophila erecta
    Drosophila yakuba
    Drosophila yakuba
    Drosophila ananassae
    Drosophila pseudoobscura pseudoobscura
    Drosophila persimilis
    Drosophila willistoni
    Drosophila virilis
    Drosophila mojavensis
    Drosophila grimshawi
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( None identified )
    No non-Drosophilid orthologies identified
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
    No non-Dipteran orthologies identified
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
    No non-Insect Arthropod orthologies identified
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Paralogs
    Paralogs (via DIOPT v7.1)
    Drosophila melanogaster (Fruit fly) (0)
    No records found.
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement

      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Subunit Structure (UniProtKB)
      Component of the Frs-CycA-Cdk1 complex composed of Z600, CycA and Cdk1. Interacts preferentially with CycA (via C-terminus) but is also able to interact (via C-terminus) with CycE (via C-terminus).
      (UniProt, P22469 )
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Gene Group - Pathway Membership (FlyBase)
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3L
      Recombination map
      3-43
      Cytogenetic map
      Sequence location
      3L:15,509,061..15,509,513 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      71D3-71D3
      Limits computationally determined from genome sequence between P{lacW}cp309s2172&P{lacW}l(3)j2A2j2A2 and P{EP}Tfb2EP572
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (6)
      Genomic Clones (16)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (23)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequences
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of
          Source for database merge of
          Source for merge of: Z600 frs
          Additional comments
          Other Comments
          Z600 controls the cell cycle pause after precisely thirteen mitoses in embryos.
          In embryos lacking Z600 function, cells in the ventral domain enter mitosis too early, causing a defective ventral furrow.
          Z600 is required for the ventral inhibition of mitosis that acts during gastrulation to counteract stg specifically in the cells that invaginate to form the mesoderm.
          In embryos lacking Z600 function, cells in the ventral domain are the first to enter mitosis, such that their pattern of stg RNA expression and the mitotic pattern match each other.
          Variation of a microsatellite within the Z600 locus has been studied in North American populations of D.melanogaster.
          The pattern of Z600 protein expression during early embryogenesis has been determined.
          The spatial and temporal expression of Z600 RNA has been studied.
          Identification: Part of an analysis of Eip71CD gene expression.
          Z600 has been identified and characterised.
          Member of cluster of genes that includes Eip28/29 and gdl. Believed to play role in processes of early development.
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 21 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          Other crossreferences
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          Flygut - An atlas of the Drosophila adult midgut
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          KEGG Genes - Molecular building blocks of life in the genomic space.
          modMine - A data warehouse for the modENCODE project
          Linkouts
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyMine - An integrated database for Drosophila genomics
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Synonyms and Secondary IDs (10)
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (70)