FB2020_06, released Dec 22, 2020

Gene: Dmel\Z600

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General Information
Symbol
Dmel\Z600
Species
D. melanogaster
Name
Z600
Annotation Symbol
CG17962
Feature Type
protein_coding_gene
FlyBase ID
FBgn0004052
Gene Model Status
Current
Stock Availability
6 publicly available
Gene Snapshot
Z600 (Z600) encodes a mitotic inhibitor that binds to the hydrophobic patch of Cyclins. It contributes to the switch in cell cycle mode in blastoderm embryos, and delaying mitosis in the mesoderm anlage to avoid an interference of mitosis and morphogenesis. [Date last reviewed: 2019-03-21]
Other Summaries
Also Known As

frs, fruhstart, DMZ60MEX

Key Links
Genomic Location
Cytogenetic map
71D3-71D3
Sequence location
3L:15,509,061..15,509,513 [+]
Recombination map

3-43

RefSeq locus
NT_037436 REGION:15509061..15509513
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (7 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables cyclin binding
inferred from physical interaction with FLYBASE:CycA; FB:FBgn0000404
(Gawlinski et al., 2007)
inferred from physical interaction with FLYBASE:CycE; FB:FBgn0010382
(Gawlinski et al., 2007)
enables protein binding
inferred from physical interaction with FLYBASE:Nup50; FB:FBgn0033264
(Gawlinski et al., 2007)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in negative regulation of G2/M transition of mitotic cell cycle
inferred from mutant phenotype
(Gawlinski et al., 2007)
involved_in negative regulation of mitotic nuclear division
inferred from mutant phenotype
(Großhans et al., 2003)
inferred from genetic interaction with FLYBASE:stg; FB:FBgn0003525
(Großhans and Wieschaus, 2000)
involved_in ventral furrow formation
inferred from genetic interaction with FLYBASE:stg; FB:FBgn0003525
(Großhans and Wieschaus, 2000)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in gastrulation
non-traceable author statement
(Lee and Orr-Weaver, 2003, Packer, 2003)
involved_in negative regulation of mitotic nuclear division
non-traceable author statement
(Packer, 2003)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Großhans et al., 2003)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in cytoplasm
non-traceable author statement
(Packer, 2003)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Protein Function (UniProtKB)
    Cell cycle regulator that is involved in modulating and adjusting cell proliferation according to the requirements of the developmental program (PubMed:10850494, PubMed:12919679, PubMed:17431409). Interacts with mitotic Cdk1-cyclin complexes to inhibit mitotic entry at the G2/M transition (PubMed:17431409). Likely to function by binding to the hydrophobic patch of cyclins to interfere with the interaction between the complex and certain Cdk1 substrates (PubMed:17431409). At the mid-blastula transition, involved in the cell cycle arrest in G2 of cycle 14 by delaying mitosis and thus reducing cell poliferation allowing cell fate specification and morphogenesis to take place (PubMed:12919679). Acts downstream or in parallel to the checkpoint regulator grp which is also required for the cell cycle pause at cycle 14 (PubMed:12919679). During gastrulation, delays mitosis in the ventral region of the embryonic mesoderm thus allowing invagination to be completed before cell division takes place (PubMed:10850494, PubMed:12919679, PubMed:17431409).
    (UniProt, P22469)
    Phenotypic Description (Red Book; Lindsley and Zimm 1992)
    Z600
    Member of cluster of genes that includes Eip28/29 and Gdl. Believed to play role in processes of early development.
    Gene Model and Products
    Number of Transcripts
    1
    Number of Unique Polypeptides
    1

    Please see the JBrowse view of Dmel\Z600 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model

    Gene model reviewed during 5.44

    Gene model reviewed during 5.46

    Sequence Ontology: Class of Gene
    nuclear_gene
    protein_coding_gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    Z600-RA
    FBtr0075549
    NM_079359
    453
    90
    Additional Transcript Data and Comments
    Reported size (kB)

    0.6 (northern blot)

    (Schulz and Butler, 1989, Schulz and Miksch, 1989)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    Z600-PA
    FBpp0075304
    10.5
    90
    10.59
    NP_524083
    AAF49634
    Polypeptides with Identical Sequences

    There is only one protein coding transcript and one polypeptide associated with this gene

    Additional Polypeptide Data and Comments
    Reported size (kDa)

    18 (kD observed); 10.5 (kD predicted)

    (Galewsky et al., 1990)
    Comments
    External Data
    Subunit Structure (UniProtKB)

    Component of the Frs-CycA-Cdk1 complex composed of Z600, CycA and Cdk1. Interacts preferentially with CycA (via C-terminus) but is also able to interact (via C-terminus) with CycE (via C-terminus).

    (UniProt, P22469)
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Z600 using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    in situ
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage 4 -- 6
    organism | subset
    (Fisher et al., 2012)
    embryonic stage 7 -- 8
    amnioserosa anlage
    (Fisher et al., 2012)
    dorsal ectoderm
    (Fisher et al., 2012)
    embryonic stage 9 -- 10
    amnioserosa primordium
    (Fisher et al., 2012)
    embryonic cycle 14
    organism | ubiquitous
    (Sung et al., 2013)
    northern blot
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage 1 -- 9

    Comment: reference states 0-4 hr AEL

    (Schulz and Miksch, 1989)
    embryonic stage 4
    organism | dorsal

    Comment: late embryonic stage 4

    (Schulz and Miksch, 1989)
    organism | posterior

    Comment: late embryonic stage 4

    (Schulz and Miksch, 1989)
    embryonic cycle 13 -- embryonic cycle 16
    (Schulz and Miksch, 1989)
    embryonic stage 5 -- 12
    organism | dorsal
    (Schulz and Miksch, 1989)
    Additional Descriptive Data

    Z600 transcripts are detected starting at embryonic cycle 13. Transcripts initially accumulate uniformly, but become concentrated in the dorsal and posterior regions during cellularization, and persist in the dorsal regions of the embryo during gastrulation and germ band extension. No signal is detected after germ band extension.

    (Schulz and Miksch, 1989)
    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    western blot
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage 1 -- 9

    Comment: reference states 0-4 hr AEL

    (Galewsky et al., 1990)
    embryonic cycle 11 -- embryonic cycle 13
    (Galewsky et al., 1990)
    Additional Descriptive Data

    The 18 kD Z600 protein is detected at low levels in 0-2 hr embryos and at higher levels in 2-4 hr embryos. No expression is detected in older embryos or in unfertilized eggs. Immunolocalization experiments reveal that expression is found only in nuclear cycle 11-13 embryos, associated with condensed chromatin. Protein is not detected at later stages, despite the presence of Z600 transcript.

    (Galewsky et al., 1990)
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    located_in cytoplasm
    inferred from direct assay
    (Großhans et al., 2003)
    Expression Deduced from Reporters
    Reporter: P{z600-lacZ}
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage 1 -- 11

    Comment: reference states 0-6 hr AEL

    (Schulz and Miksch, 1989)
    embryonic stage 4
    organism | dorsal

    Comment: late embryonic stage 4

    (Schulz and Miksch, 1989)
    organism | posterior

    Comment: late embryonic stage 4

    (Schulz and Miksch, 1989)
    organism | ventral

    Comment: late embryonic stage 4

    (Schulz and Miksch, 1989)
    embryonic cycle 13 -- embryonic cycle 16
    (Schulz and Miksch, 1989)
    embryonic stage 5 -- 12
    organism | dorsal
    (Schulz and Miksch, 1989)
    organism | ventral
    (Schulz and Miksch, 1989)
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\Z600 in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Bulk Downloads
    RNA-Seq RPKM values for all genes
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Flygut - An atlas of the Drosophila adult midgut
    Images
    FlyBase Wiki
    Image Based Resources
    Alleles, Insertions, and Transgenic Constructs
    Classical and Insertion Alleles ( 1 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 12 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of Z600
    Transgenic constructs containing regulatory region of Z600
    Deletions and Duplications ( 9 )
    Phenotypes
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Download All OrthoDB Orthologs
    Human Orthologs (via DIOPT v8.0)
    Homo sapiens (Human) (0)
    No records found.
    Model Organism Orthologs (via DIOPT v8.0)
    Mus musculus (laboratory mouse) (0)
    No records found.
    Rattus norvegicus (Norway rat) (0)
    No records found.
    Xenopus tropicalis (Western clawed frog) (0)
    No records found.
    Danio rerio (Zebrafish) (0)
    No records found.
    Caenorhabditis elegans (Nematode, roundworm) (0)
    No records found.
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190K3C )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila suzukii
    Spotted wing Drosophila
    Dsuz\DS10_00012932
    Drosophila simulans
    Dsim\GD14545
    Drosophila sechellia
    Dsec\GM25530
    Drosophila erecta
    Dere\GG15901
    Drosophila yakuba
    Dyak\GE22242
    Drosophila yakuba
    Dyak\GE23146
    Drosophila ananassae
    Dana\GF23702
    Drosophila pseudoobscura pseudoobscura
    Dpse\GA14745
    Drosophila persimilis
    Dper\GL24823
    Drosophila willistoni
    Dwil\GK10594
    Drosophila virilis
    Dvir\GJ13860
    Drosophila mojavensis
    Dmoj\GI13113
    Drosophila grimshawi
    Dgri\GH14542
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( None identified )
    No non-Drosophilid orthologies identified
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
    No non-Dipteran orthologies identified
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
    No non-Insect Arthropod orthologies identified
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v8.0)
    Drosophila melanogaster (Fruit fly) (0)
    No records found.
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement
      View in Interactions Browser
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Z600
      suppressible
      CycE
      (Gawlinski et al., 2007)
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Cdk1
      suppressible
      Z600
      (Gawlinski et al., 2007)
      External Data
      Subunit Structure (UniProtKB)
      Component of the Frs-CycA-Cdk1 complex composed of Z600, CycA and Cdk1. Interacts preferentially with CycA (via C-terminus) but is also able to interact (via C-terminus) with CycE (via C-terminus).
      (UniProt, P22469 )
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3L
      Recombination map

      3-43

      Cytogenetic map
      71D3-71D3
      Sequence location
      3L:15,509,061..15,509,513 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      71D3-71D3
      Limits computationally determined from genome sequence between P{lacW}cp309s2172&P{lacW}l(3)j2A2j2A2 and P{EP}Tfb2EP572
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      71C-71C
      (Gibson et al., 1999)
      Experimentally Determined Recombination Data
      Location

      3-43

      (FlyBase, 2016)

      3-37.2

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (6)
      Genomic Clones (16)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (23)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           

          polyclonal

          (Galewsky et al., 1990)
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of
          Source for database merge of

          Source for merge of: Z600 frs

          (Großhans et al., 2003)
          Additional comments
          Other Comments

          Z600 controls the cell cycle pause after precisely thirteen mitoses in embryos.

          (Großhans et al., 2003)

          In embryos lacking Z600 function, cells in the ventral domain enter mitosis too early, causing a defective ventral furrow.

          (Großhans et al., 2003)

          Z600 is required for the ventral inhibition of mitosis that acts during gastrulation to counteract stg specifically in the cells that invaginate to form the mesoderm.

          (Großhans and Wieschaus, 2000)

          In embryos lacking Z600 function, cells in the ventral domain are the first to enter mitosis, such that their pattern of stg RNA expression and the mitotic pattern match each other.

          (Großhans and Wieschaus, 2000)

          Variation of a microsatellite within the Z600 locus has been studied in North American populations of D.melanogaster.

          (Goldstein and Clark, 1995)

          The pattern of Z600 protein expression during early embryogenesis has been determined.

          (Galewsky et al., 1990)

          The spatial and temporal expression of Z600 RNA has been studied.

          (Schulz and Miksch, 1989)

          Identification: Part of an analysis of Eip71CD gene expression.

          (Schulz et al., 1989)

          Z600 has been identified and characterised.

          (Schulz et al., 1989)

          Member of cluster of genes that includes Eip28/29 and gdl. Believed to play role in processes of early development.

          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 22 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          Other crossreferences
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          Flygut - An atlas of the Drosophila adult midgut
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          KEGG Genes - Molecular building blocks of life in the genomic space.
          modMine - A data warehouse for the modENCODE project
          Linkouts
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          FlyMine - An integrated database for Drosophila genomics
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Synonyms and Secondary IDs (10)
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (72)