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Gene Dmel\bs

General Information
Symbol	Dmel\bs	Species	D. melanogaster
Name	blistered	Annotation symbol	CG3411
Feature type	protein_coding_gene	FlyBase ID	FBgn0004101
Gene Model Status	Current	Stock availability	21 publicly available
Also Known As	DSRF, SRF, D-SRF, bs/DSRF, pruned/DSRF, ba
Genomic Location
Chromosome (arm)	2R	Recombination map	2-107.3
Cytogenetic map	60C6-60C7	Sequence location	2R:20,230,389..20,263,998 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene blistered is referred to in FlyBase by the symbol Dmel\bs (CG3411, FBgn0004101). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; activating transcription factor binding. There is experimental evidence that it is involved in the biological process: apposition of dorsal and ventral imaginal disc-derived wing surfaces; positive regulation of transcription from RNA polymerase II promoter; imaginal disc-derived wing vein morphogenesis; terminal branching, open tracheal system; imaginal disc-derived wing morphogenesis; sleep; open tracheal system development. 60 alleles are reported. The phenotypes of these alleles are annotated with: adult segment; organ system subdivision; organ system; adult mesothoracic segment; tracheal system; thoracic segment; appendage segment; embryonic/larval tracheal system; sensillum; non-connected developing system; cephalopharyngeal skeleton. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Armadillo-like helical; Transcription factor, MADS-box. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of low expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval/adult midgut, larval Malpighian tubules, larval fat body, larval/adult salivary gland, adult ovary, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1630990_at completely aligns to an exonic region of only a subset of 1 of the 2 FlyBase-annotated transcript isoforms of bs. Gene sequence location is 2R:20230389..20263998.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	bs: blistered bs2: blistered-2 Edith M. Wallace, unpublished. Wings blistered, small, pointed; venation thick and plexus-like with branches from and parallel to L5 beyond second crossvein, where there is a semidominant free vein effect. Temperature sensitive. RK2 at 19; RK3 at 25. ba: balloon ba: balloon From Bridges and Morgan, 1919, Carnegie Inst. Washington Publ. No. 278: 148. Wings at first inflated with hemolymph to produce blisters and vesicles; venation weak, plexus like; wings smaller, warped, discolored, and divergent. Effect caused by inadequate contraction of epithelium after inflated state of pupal wing [Waddington, 1940, J. Genet. 41: 75-139 (fig.)]. Sensitive to temperature. RK3 above 25; RK2 at 19 or below.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Clones RE37645 EK092504 11693
FB2012_01	Sequence features BKN28659 HFA04676 Controlled Vocabulary Terms imaginal disc-derived wing vein morphogenesis positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription activating transcription factor binding
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 60C6-60C7   Limits computationally determined from genome sequence between P{lacW}Nop60Bk05318 and P{PZ}bs03267&P{lacW}l(2)k10502k10502
Experimentally Determined Cytological Location
Cytogenetic map Notes References 60C-60C     (Groppe, 1994.2.3) 60C6-60C8   (determined by in situ hybridisation)   (Spradling et al., 1999) 60C6-60C8     (Beaton, 1997.12.27) 60C-60C   (determined by in situ hybridisation)   (Guillemin et al., 1996) 60C-60C   (determined by in situ hybridisation)   (Affolter et al., 1994) 60C6-60C8   60C7--8   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2-107.3 (Goldschmidt, 1944)
Left of (cM)	Kr (Fristrom et al., 1994)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\bs for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.42 Transposon inserted in intron
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) bs-RA FBtr0072271  NM_166667   2235   449 bs-RB FBtr0290089  NM_001144279   3355   355
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein bs-PA   FBpp0072178   47.6   449   7.06     NP_726438   AAF47195 bs-PB   FBpp0288528   38.7   355   9.33     NP_001137751   ACL83204
Additional Polypeptide Data & Comments
Reported size (kDa)	450 (aa) (Affolter et al., 1994)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Transcription factor, MADS-box (IPR002100) Armadillo-like helical (IPR011989)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA140663   AA140932   AA141121   AA141124   AA141143   AA141196   AA141199   AA141201   AA141234   AA141323   AA141354   AA141371   AA141408   AA141422   AA141464   AA141478   AA141489   AA141497   AA141560   AA141562   AA141607   AA141609   AA141611   AA141655   AA141676   AA141689   AA141707   AA141711   AA141735   AA141743   AA141801   AA141834   AA141881   AA142178   AA440163   AE013599 AAF47195   AE013599 ACL83204   AI124308   AI296338   AI386899   AI387761   AI531290   AY095071 AAM11399   BI142244   BI165760   BI212701   BI239115   BI240731   BI352942   BI358181   BI362915   BI369714   BI374217   BI564851   BI565487   BI571886   BI574131   BI574442   BI575214   BI576026   BI577030   BI582454   BI583280   BI583851   BI583993   BI586399   BI586725   BI586861   BI588375   BI588406   BI588459   BI588663   BI588766   BI588884   BI589090   BI589239   BI589243   BI590672   BI590731   BI592794   BI593032   BI604445   BI605511   BI606547   BI607294   BI607877   BI607977   BI608784   BI608982   BI610522   BI610661   BI611018   BI611097   BI611159   BI611230   BI611710   BI612977   BI613020   BI613394   BI613754   BI615370   BI615509   BI616145   BI616581   BI616610   BI616906   BI617028   BI617090   BI617439   BI617684   BI618824   BI619653   BI620053   BI620538   BI620781   BI621147   BI621284   BI622500   BI622740   BI623088   BI623093   BI623319   BI623627   BI624745   BI625317   BI625766   BI625785   BI625802   BI626350   BI626426   BI626450   BI626871   BI627441   BI627518   BI628367   BI628419   BI628426   BI628436   BI628544   BI629001   BI629190   BI629211   BI629381   BI630149   BI630924   BI631188   BI631763   BI631785   BI631848   BI631995   BI634197   BI634339   BI634950   BI636622   BI640653   BI641728   CK135090   CK658320   CO153056   CO153494   CO177555   CO179042   CO179169   CO179305   CO187196   CO187484   CO187678   CO187710   CO187913   CO189129   CO190350   CO190469   CO190773   CO190982   CO195863   CO261955   CO262568   CO263244   CO263592   CO264634   CO266208   CO266621   CO267454   CO268027   CO268650   CO268713   CO269696   CO271850   CO271971   CO273023   CO273774   CO274206   CO274311   CO274786   CO275125   CO275338   CO275570   CO275968   CO276551   CO278383   CO278479   CO279082   CO280443   CO281955   CO281966   CO282275   CO282855   CO284008   CO284265   CO284404   CO290009   CO290180   CO290679   CO290759   CO291316   CO291587   CO291788   CO291860   CO292855   CO293512   CO293878   CO294202   CO294540   CO295238   CO296496   CO297010   CO297521   CO297551   CO300478   CO302049   CO302309   CO302349   CO302681   CO303318   CO303356   CO303980   CO304946   CO307131   CO308453   CO309390   CO309805   CO310496   CO311647   CO311693   CO311933   CO312319   CO315577   CO315650   CO316667   CO317096   CO317213   CO317355   CO319487   CO319523   CO320976   CO320977   CO321140   CO322033   CO324658   CO324776   CO326836   CO326862   CO327048   CO327604   CO328187   CO328257   CO328314   CO328871   CO329111   CO330795   CO331792   CO332493   CO332733   CO337673   CO338920   CO339169   CO339604   CO340021   CO340225   CO340355   CO340387   CO340851   CO340882   CO340913   CO340947   CO341122   CO341778   CO341986   CO342194   CO342406   CO343368   CO343580   CO343721   CO344264   CO344379   CO344766   CO344971   CZ472194   CZ473688   CZ474566   CZ474850   CZ474851   EC053545   EC079013   EC085318   EC209454   EC211232   EC225217   EC236219   EC243903   EC259425   G00561   GH760196   GH826139   GH826548   GH921730   S81937   X77532 CAA54670
UniProtKB/Swiss-Prot	Q24535
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References protein binding site FBsf0000160770 2R:20,229,832..20,229,857 bound_moiety=sd-XP evidence=experimental (Bergman et al., 2004, Halder et al., 1998)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 14 -- 17   embryonic/larval hindgut   (Affolter et al., 1994) presumptive embryonic/larval central nervous system | restricted   (Affolter et al., 1994) pharyngeal muscle   (Affolter et al., 1994) embryonic/larval midgut   (Affolter et al., 1994) metathoracic segment | restricted   (Affolter et al., 1994) abdominal segment 1 -- abdominal segment 8   (Affolter et al., 1994) mesothoracic segment | restricted   (Affolter et al., 1994) embryonic stage 16   embryonic/larval somatic muscle   (Affolter et al., 1994)
Additional Descriptive Data
	bs transcripts are expressed ubiquitously in unfertilized eggs. The RNA fades away during germ band extension and zygotic expression is not detected until germ band retraction. At stage 14, expression is observed just beneath the epidermis in a cluster of 6-9 cells in the most anterior portion of each hemisegment from T2 to A8. Expression is also observed in the head, in two rows of cells along the midgut and in a single cell in each hemisegment of the CNS. Other sites include 1 to 2 cells in the dorsal part of each hemisegment from T to A6, cells along the pharyngeal muscle and along the hindgut. In late embryos, weaker expression is observed in somatic muscles. (Affolter et al., 1994)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 14 -- 17   mesothoracic segment | restricted   (Affolter et al., 1994) metathoracic segment | restricted   (Affolter et al., 1994) presumptive embryonic/larval central nervous system | restricted   (Affolter et al., 1994) embryonic/larval hindgut   (Affolter et al., 1994) pharyngeal muscle   (Affolter et al., 1994) embryonic/larval midgut   (Affolter et al., 1994) abdominal segment 1 -- abdominal segment 8   (Affolter et al., 1994) embryonic stage 16   embryonic/larval somatic muscle   (Affolter et al., 1994) third instar larval stage   wing pouch   (Grenier and Carroll, 2000) Comment:NOT presumptive wing veins, NOT dorsal/ventral compartment boundary pupal stage   wing   (Johannes and Preiss, 2002) Comment:NOT wing vein
Additional Descriptive Data
	bs protein is expressed ubiquitously in unfertilized eggs. The protein fades away during germ band extension and zygotic expression is not detected until germ band retraction. At stage 14, expression is observed just beneath the epidermis in a cluster of 6-9 cells in the most anterior portion of each hemisegment from T2 to A8. Expression is also observed in the head, in two rows of cells along the midgut and in a single cell in each hemisegment of the CNS. Other sites include 1 to 2 cells in the dorsal part of each hemisegment from T2 to A6, cells along the pharyngeal muscle and along the hindgut. In late embryos, weaker expression is observed in somatic muscles. Using double staining techniques, it was shown that all of the cells that express bs strongly are tightly associated with the tracheal system. (Affolter et al., 1994) Expression of bs occurs in the intervein tissue of the developing pupal wing in a pattern complementary to that of rho. (Johannes and Preiss, 2002)
Marker for
Subcellular Localization
CV Term	nucleus (Affolter et al., 1994)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0004101    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0004101 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of low expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0004101 expression level Developmental Stage   Expression Level embryo 00-02hr    1389 embryo 02-04hr    406 embryo 04-06hr    153 embryo 06-08hr    218 embryo 08-10hr    230 embryo 10-12hr    407 embryo 12-14hr    623 embryo 14-16hr    815 embryo 16-18hr    765 embryo 18-20hr    815 embryo 20-22hr    379 embryo 22-24hr    344 larva L1    380 larva L2    343 larva L3 12hr old    298 larva L3 puffstage 1-2    227 larva L3 puffstage 3-6    380 larva L3 puffstage 7-9    484 white prepupae new    431 white prepupae 12hr    346 white prepupae 24hr    685 pupae 2d postWPP    558 pupae 3d postWPP    626 pupae 4d postWPP    362 adult male 01day    283 adult male 05day    287 adult male 30day    232 adult female 01day    488 adult female 05day    689 adult female 30day    754 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1389) embryo 02-04hr  (406) embryo 04-06hr  (153) embryo 06-08hr  (218) embryo 08-10hr  (230) embryo 10-12hr  (407) embryo 12-14hr  (623) embryo 14-16hr  (815) embryo 16-18hr  (765) embryo 18-20hr  (815) embryo 20-22hr  (379) embryo 22-24hr  (344) larva L1  (380) larva L2  (343) larva L3 12hr old  (298) larva L3 puffstage 1-2  (227) larva L3 puffstage 3-6  (380) larva L3 puffstage 7-9  (484) white prepupae new  (431) white prepupae 12hr  (346) white prepupae 24hr  (685) pupae 2d postWPP  (558) pupae 3d postWPP  (626) pupae 4d postWPP  (362) adult male 01day  (283) adult male 05day  (287) adult male 30day  (232) adult female 01day  (488) adult female 05day  (689) adult female 30day  (754) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1389 embryo 02-04hr    406 embryo 04-06hr    153 embryo 06-08hr    218 embryo 08-10hr    230 embryo 10-12hr    407 embryo 12-14hr    623 embryo 14-16hr    815 embryo 16-18hr    765 embryo 18-20hr    815 embryo 20-22hr    379 embryo 22-24hr    344 larva L1    380 larva L2    343 larva L3 12hr old    298 larva L3 puffstage 1-2    227 larva L3 puffstage 3-6    380 larva L3 puffstage 7-9    484 white prepupae new    431 white prepupae 12hr    346 white prepupae 24hr    685 pupae 2d postWPP    558 pupae 3d postWPP    626 pupae 4d postWPP    362 adult male 01day    283 adult male 05day    287 adult male 30day    232 adult female 01day    488 adult female 05day    689 adult female 30day    754 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1389 embryo 02-04hr    406 embryo 04-06hr    153 embryo 06-08hr    218 embryo 08-10hr    230 embryo 10-12hr    407 embryo 12-14hr    623 embryo 14-16hr    815 embryo 16-18hr    765 embryo 18-20hr    815 embryo 20-22hr    379 embryo 22-24hr    344 larva L1    380 larva L2    343 larva L3 12hr old    298 larva L3 puffstage 1-2    227 larva L3 puffstage 3-6    380 larva L3 puffstage 7-9    484 white prepupae new    431 white prepupae 12hr    346 white prepupae 24hr    685 pupae 2d postWPP    558 pupae 3d postWPP    626 pupae 4d postWPP    362 adult male 01day    283 adult male 05day    287 adult male 30day    232 adult female 01day    488 adult female 05day    689 adult female 30day    754 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1389 embryo 02-04hr    406 embryo 04-06hr    153 embryo 06-08hr    218 embryo 08-10hr    230 embryo 10-12hr    407 embryo 12-14hr    623 embryo 14-16hr    815 embryo 16-18hr    765 embryo 18-20hr    815 embryo 20-22hr    379 embryo 22-24hr    344 larva L1    380 larva L2    343 larva L3 12hr old    298 larva L3 puffstage 1-2    227 larva L3 puffstage 3-6    380 larva L3 puffstage 7-9    484 white prepupae new    431 white prepupae 12hr    346 white prepupae 24hr    685 pupae 2d postWPP    558 pupae 3d postWPP    626 pupae 4d postWPP    362 adult male 01day    283 adult male 05day    287 adult male 30day    232 adult female 01day    488 adult female 05day    689 adult female 30day    754 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0004101 expression level Developmental Stage   Expression Level embryo 00-02hr    1389 embryo 02-04hr    406 embryo 04-06hr    153 embryo 06-08hr    218 embryo 08-10hr    230 embryo 10-12hr    407 embryo 12-14hr    623 embryo 14-16hr    815 embryo 16-18hr    765 embryo 18-20hr    815 embryo 20-22hr    379 embryo 22-24hr    344 larva L1    380 larva L2    343 larva L3 12hr old    298 larva L3 puffstage 1-2    227 larva L3 puffstage 3-6    380 larva L3 puffstage 7-9    484 white prepupae new    431 white prepupae 12hr    346 white prepupae 24hr    685 pupae 2d postWPP    558 pupae 3d postWPP    626 pupae 4d postWPP    362 adult male 01day    283 adult male 05day    287 adult male 30day    232 adult female 01day    488 adult female 05day    689 adult female 30day    754 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1389) embryo 02-04hr  (406) embryo 04-06hr  (153) embryo 06-08hr  (218) embryo 08-10hr  (230) embryo 10-12hr  (407) embryo 12-14hr  (623) embryo 14-16hr  (815) embryo 16-18hr  (765) embryo 18-20hr  (815) embryo 20-22hr  (379) embryo 22-24hr  (344) larva L1  (380) larva L2  (343) larva L3 12hr old  (298) larva L3 puffstage 1-2  (227) larva L3 puffstage 3-6  (380) larva L3 puffstage 7-9  (484) white prepupae new  (431) white prepupae 12hr  (346) white prepupae 24hr  (685) pupae 2d postWPP  (558) pupae 3d postWPP  (626) pupae 4d postWPP  (362) adult male 01day  (283) adult male 05day  (287) adult male 30day  (232) adult female 01day  (488) adult female 05day  (689) adult female 30day  (754) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1389 embryo 02-04hr    406 embryo 04-06hr    153 embryo 06-08hr    218 embryo 08-10hr    230 embryo 10-12hr    407 embryo 12-14hr    623 embryo 14-16hr    815 embryo 16-18hr    765 embryo 18-20hr    815 embryo 20-22hr    379 embryo 22-24hr    344 larva L1    380 larva L2    343 larva L3 12hr old    298 larva L3 puffstage 1-2    227 larva L3 puffstage 3-6    380 larva L3 puffstage 7-9    484 white prepupae new    431 white prepupae 12hr    346 white prepupae 24hr    685 pupae 2d postWPP    558 pupae 3d postWPP    626 pupae 4d postWPP    362 adult male 01day    283 adult male 05day    287 adult male 30day    232 adult female 01day    488 adult female 05day    689 adult female 30day    754 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1389 embryo 02-04hr    406 embryo 04-06hr    153 embryo 06-08hr    218 embryo 08-10hr    230 embryo 10-12hr    407 embryo 12-14hr    623 embryo 14-16hr    815 embryo 16-18hr    765 embryo 18-20hr    815 embryo 20-22hr    379 embryo 22-24hr    344 larva L1    380 larva L2    343 larva L3 12hr old    298 larva L3 puffstage 1-2    227 larva L3 puffstage 3-6    380 larva L3 puffstage 7-9    484 white prepupae new    431 white prepupae 12hr    346 white prepupae 24hr    685 pupae 2d postWPP    558 pupae 3d postWPP    626 pupae 4d postWPP    362 adult male 01day    283 adult male 05day    287 adult male 30day    232 adult female 01day    488 adult female 05day    689 adult female 30day    754 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1389 embryo 02-04hr    406 embryo 04-06hr    153 embryo 06-08hr    218 embryo 08-10hr    230 embryo 10-12hr    407 embryo 12-14hr    623 embryo 14-16hr    815 embryo 16-18hr    765 embryo 18-20hr    815 embryo 20-22hr    379 embryo 22-24hr    344 larva L1    380 larva L2    343 larva L3 12hr old    298 larva L3 puffstage 1-2    227 larva L3 puffstage 3-6    380 larva L3 puffstage 7-9    484 white prepupae new    431 white prepupae 12hr    346 white prepupae 24hr    685 pupae 2d postWPP    558 pupae 3d postWPP    626 pupae 4d postWPP    362 adult male 01day    283 adult male 05day    287 adult male 30day    232 adult female 01day    488 adult female 05day    689 adult female 30day    754 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0004101    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0004101 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval/adult midgut, larval Malpighian tubules, larval fat body, larval/adult salivary gland, adult ovary, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0004101 expression level Tissue   Expression Level Larval Central Nervous System    169.8 Larval Midgut    102.9 Larval Hindgut    76.9 Larval Malpighian Tubules    108.8 Larval Fat Body    100.4 Larval Salivary Gland    163.6 Larval Trachea    67.575 Larval Carcass    118.225 Adult Head    67.6 Adult Eye    58.1 Adult Brain    114.3 Adult Thoracic-Abdominal Ganglion    102.3 Adult Crop    71.7 Adult Midgut    107.1 Adult Hindgut    54 Adult Malpighian Tubules no informative data Adult Fat Body    92.3 Adult Salivary Gland    100.3 Adult Heart    65.2 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    301.3 Adult Testis    30.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    169.8 Larval Midgut    102.9 Larval Hindgut    76.9 Larval Malpighian Tubules    108.8 Larval Fat Body    100.4 Larval Salivary Gland    163.6 Larval Trachea    67.575 Larval Carcass    118.225 Adult Head    67.6 Adult Eye    58.1 Adult Brain    114.3 Adult Thoracic-Abdominal Ganglion    102.3 Adult Crop    71.7 Adult Midgut    107.1 Adult Hindgut    54 Adult Malpighian Tubules no informative data Adult Fat Body    92.3 Adult Salivary Gland    100.3 Adult Heart    65.2 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    301.3 Adult Testis    30.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    169.8 Larval Midgut    102.9 Larval Hindgut    76.9 Larval Malpighian Tubules    108.8 Larval Fat Body    100.4 Larval Salivary Gland    163.6 Larval Trachea    67.575 Larval Carcass    118.225 Adult Head    67.6 Adult Eye    58.1 Adult Brain    114.3 Adult Thoracic-Abdominal Ganglion    102.3 Adult Crop    71.7 Adult Midgut    107.1 Adult Hindgut    54 Adult Malpighian Tubules no informative data Adult Fat Body    92.3 Adult Salivary Gland    100.3 Adult Heart    65.2 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    301.3 Adult Testis    30.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    169.8 Larval Midgut    102.9 Larval Hindgut    76.9 Larval Malpighian Tubules    108.8 Larval Fat Body    100.4 Larval Salivary Gland    163.6 Larval Trachea    67.575 Larval Carcass    118.225 Adult Head    67.6 Adult Eye    58.1 Adult Brain    114.3 Adult Thoracic-Abdominal Ganglion    102.3 Adult Crop    71.7 Adult Midgut    107.1 Adult Hindgut    54 Adult Malpighian Tubules no informative data Adult Fat Body    92.3 Adult Salivary Gland    100.3 Adult Heart    65.2 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    301.3 Adult Testis    30.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0004101 expression level Tissue   Expression Level Larval Central Nervous System    169.8 Larval Midgut    102.9 Larval Hindgut    76.9 Larval Malpighian Tubules    108.8 Larval Fat Body    100.4 Larval Salivary Gland    163.6 Larval Trachea    67.575 Larval Carcass    118.225 Adult Head    67.6 Adult Eye    58.1 Adult Brain    114.3 Adult Thoracic-Abdominal Ganglion    102.3 Adult Crop    71.7 Adult Midgut    107.1 Adult Hindgut    54 Adult Malpighian Tubules no informative data Adult Fat Body    92.3 Adult Salivary Gland    100.3 Adult Heart    65.2 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    301.3 Adult Testis    30.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    169.8 Larval Midgut    102.9 Larval Hindgut    76.9 Larval Malpighian Tubules    108.8 Larval Fat Body    100.4 Larval Salivary Gland    163.6 Larval Trachea    67.575 Larval Carcass    118.225 Adult Head    67.6 Adult Eye    58.1 Adult Brain    114.3 Adult Thoracic-Abdominal Ganglion    102.3 Adult Crop    71.7 Adult Midgut    107.1 Adult Hindgut    54 Adult Malpighian Tubules no informative data Adult Fat Body    92.3 Adult Salivary Gland    100.3 Adult Heart    65.2 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    301.3 Adult Testis    30.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    169.8 Larval Midgut    102.9 Larval Hindgut    76.9 Larval Malpighian Tubules    108.8 Larval Fat Body    100.4 Larval Salivary Gland    163.6 Larval Trachea    67.575 Larval Carcass    118.225 Adult Head    67.6 Adult Eye    58.1 Adult Brain    114.3 Adult Thoracic-Abdominal Ganglion    102.3 Adult Crop    71.7 Adult Midgut    107.1 Adult Hindgut    54 Adult Malpighian Tubules no informative data Adult Fat Body    92.3 Adult Salivary Gland    100.3 Adult Heart    65.2 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    301.3 Adult Testis    30.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    169.8 Larval Midgut    102.9 Larval Hindgut    76.9 Larval Malpighian Tubules    108.8 Larval Fat Body    100.4 Larval Salivary Gland    163.6 Larval Trachea    67.575 Larval Carcass    118.225 Adult Head    67.6 Adult Eye    58.1 Adult Brain    114.3 Adult Thoracic-Abdominal Ganglion    102.3 Adult Crop    71.7 Adult Midgut    107.1 Adult Hindgut    54 Adult Malpighian Tubules no informative data Adult Fat Body    92.3 Adult Salivary Gland    100.3 Adult Heart    65.2 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    301.3 Adult Testis    30.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules no informative data Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    67.6   NA  Eye    58.1   NA  Brain    114.3   169.8  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    102.3   NA  Crop    71.7   102.9  Midgut    107.1   76.9  Hindgut    54   108.8  Malpighian Tubules    no informative data   100.4  Fat Body    92.3   163.6  Salivary Gland    100.3   NA  Heart    65.2   67.575  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    301.3   NA  Testis    30.8   NA  Male Accessory Gland    no informative data   118.225  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_26
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004101 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3411-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage (with Df(2R)Px2) bsEbr292 lethal | embryonic stage | recessive bsEbr292 lethal | larval stage bs14 bs364 lethal | larval stage | recessive bs2R14 bs13 bs14 bs221 bs242 bsEbr292 bsunspecified lethal | pharate adult stage | recessive bsex53 bsex87 lethal | pupal stage | recessive bsex84 lethal | recessive bs2R-19 bs2R19 bs03267 bsk07909 bsV100 bsV121 viable bs12 bs211 bs237 bs246 bscy bsF31 bsGod bsP viable (with bs2) bs03267 viable (with bs03267) bs2 viable | poor bsbl bsPP Sterility Allele fertile | poor bsbl Other Phenotypes Allele courtship behavior defective, with Scer\GAL80cry.PS bsScer\UAS.cHa decreased cell size | cell autonomous | somatic clone bs14 memory defective bs1348 visible bs2 bs12 bsEbr292 visible, with Scer\GAL4Bx-MS1096 bsScer\UAS.cHa visible (with bs2) bs03267 visible (with bs03267) bs2 visible | dominant bs2R14 bs2R19 bs14 bs03267 bsk07909 visible | dominant | somatic clone bsunspecified visible | recessive bs2 bs3 bs4 bs11 bs12 bs52d bs54j bs03267 bsA48 bsba-2 bsba bsbl bsE111 bsF31 visible | recessive | heat sensitive bs1 bsba visible | semidominant bs364 bscy visible | somatic clone bsV100 bsV121 wild-type bsex3 bsex8 bsex40 Phenotype manifest in Allele border follicle cell | somatic clone bs2R14 cephalopharyngeal skeleton bsEbr292 cephalopharyngeal skeleton (with Df(2R)Px2) bsEbr292 chaeta bs2R14 embryonic/first instar larval cuticle bsEbr292 embryonic/first instar larval cuticle (with Df(2R)Px2) bsEbr292 embryonic/larval tracheal system bs03267 bsEbr292 embryonic/larval tracheal system | somatic clone bs03267 femur bs03267 follicle cell & actin filament | somatic clone bs2R14 macrochaeta | ectopic bs03267 presumptive embryonic/larval tracheal system bs14 rhabdomere | somatic clone bsk07909 sensillum campaniformium | ectopic bs03267 submarginal cell bs11 tarsal segment bs03267 trachea bs03267 bs14 bsex53 bsex84 bsex87 tracheal metamere 3 bs03267 tracheal metamere 3 | somatic clone bs03267 wing bs03267 bs1 bs11 bs12 bs13 bs2 bs2R19 bs3 bs4 bs52d bs54j bsA48 bsE111 bsEbr292 bsF31 bsGod bsP bsba bsba-2 bsbl bscy bsk07909 wing (with bs2) bs03267 wing (with bs03267) bs2 wing & macrochaeta bs03267 wing | P-stage bsba wing | somatic clone bs14 bsV100 bsV121 bsunspecified wing cell bs03267 bs12 wing cell, with Scer\GAL4Bx-MS1096 bsScer\UAS.cHa wing cell (with bs217) bs242 wing cell (with bs242) bs217 wing disc | P-stage (with In(2LR)Px4) bs11 wing sensillum | ectopic bs03267 wing vein bs1 bs11 bs12 bs13 bs14 bs2 bs2R14 bs2R19 bsA48 bsF31 bsGod bsP bsPx bsba bsbl bscy wing vein, with Scer\GAL4Bx-MS1096 bsScer\UAS.cHa wing vein | cell non-autonomous | somatic clone bs14 wing vein | ectopic bs03267 bs11 bs12 bs14 bs2 bs364 bsA48 bsk07909 wing vein | ectopic (with bs2) bs03267 wing vein | ectopic (with bs217) bs242 wing vein | ectopic (with bs242) bs217 wing vein | ectopic (with bs03267) bs2 wing vein L3 & sensillum campaniformium bs03267
Classical Alleles ( 55 )
For All Classical Alleles Show
Allele of bs Class Mutagen Stocks Known lesion bs2 3 -- bs3 spontaneous 3 -- bs1348 P-element activity 2 Yes bsba spontaneous 2 -- bsk07909 P-element activity 2 Yes bs4 spontaneous 1 -- bsA48 ethyl methanesulfonate 1 -- bsd01376 P-element activity 1 -- bsd05493 P-element activity 1 -- bsEY23316 P-element activity 1 -- bsMB00417 1 -- bsMI01549 1 -- bs14 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes bsex84 amorphic allele - genetic evidence Delta2-3 0 -- bs03267 loss of function allele P-element activity 0 Yes bs11 ethyl methanesulfonate 0 Yes bs12 spontaneous 0 -- bs13 ethyl methanesulfonate 0 -- bs1 spontaneous 0 -- bs211 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- bs217 0 -- bs221 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- bs237 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- bs242 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- bs246 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- bs253 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- bs2R-19 X ray 0 -- bs2R14 0 -- bs2R19 0 Yes bs364 X ray 0 -- bs38i 0 -- bs52d spontaneous 0 -- bs54j spontaneous 0 -- bs8 0 -- bsba-2 spontaneous 0 -- bsbl spontaneous 0 -- bscy spontaneous 0 -- bsd04015 P-element activity 0 -- bsd05688 0 -- bsd05976 P-element activity 0 -- bsE111 0 -- bsEbr292 ethyl methanesulfonate 0 -- bsex3 Delta2-3 0 -- bsex40 Delta2-3 0 -- bsex53 Delta2-3 0 -- bsex87 Delta2-3 0 -- bsex8 Delta2-3 0 -- bsF31 ethyl methanesulfonate 0 -- bsGod ethyl methanesulfonate 0 -- bsP spontaneous 0 -- bsPP spontaneous 0 -- bsPx 0 -- bsunspecified X ray 0 -- bsV100 X ray 0 -- bsV121 X ray 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of bs Class Mutagen Stocks Known lesion bsJF02319 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes bsKK108659 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes bsdsRNA.cCa in vitro construct - RNAi 0 Yes bsScer\UAS.cHa in vitro construct - regulatory fusion 0 Yes bsT:Hsap\MYC in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)BSC780 (Christensen et al., 2009.5.6) Df(2R)bs-F31 (Fristrom et al., 1994) Df(2R)bwVDe2LPxKR (Spradling et al., 1999, BDGP Project Members, 1994-1999, Beaton, 1997.12.27) Df(2R)DE1 (Montagne et al., 1996) Df(2R)Pin-R10-1 (Reed, 1992) Df(2R)Px1 (Bridges, 1937, Affolter et al., 1994, Guillemin et al., 1996) Df(2R)Px2 (Walsh and Brown, 1998, Spradling et al., 1999, LaJeunesse et al., 2001, Bridges, 1937, Kimble et al., 1990, Diaz-Benjumea and Garcia-Bellido, 1990, Gotwals and Fristrom, 1991, BDGP Project Members, 1994-1999, Affolter et al., 1994, Fristrom et al., 1994, Guillemin et al., 1996, Montagne et al., 1996) Df(2R)Px5 (Thompson, 1963) In(2LR)Px4 (Spradling et al., 1999, Kimble et al., 1990, BDGP Project Members, 1994-1999, Guillemin et al., 1996, Montagne et al., 1996, Prout et al., 1997) T(2;3)God1 (Ruden and Jaeckle, 1995) Ts(1Lt;2Lt)Bld
Duplicated in	Dp(2;3)P
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK108659} NA P{TRiP.JF02319} NA P{UAS-bs.H} NA reporter construct P{bs-GAL4.C} No P{bs-lacZ.A} No P{bs-lacZ.Amut} No P{bs-lacZ.HZ2.7} No P{bs-lacZ.HZ3.1} No P{bs-lacZ.HZ3.8} No P{bs-lacZ.HZ4.4} No P{bs-lacZ.pCβ0.5} No P{bs-lacZ.pCβ0.6} No P{bs-lacZ.pCβ0.7} No P{bs-lacZ.pCβ1.6} No P{bs-lacZ.pCβ1.7} No P{bs-lacZ.pCβ1.8} No P{bs-lacZ.pCβ6.2} No characterization construct P{bs-GAL4.Term} NA P{bs-lacZ.pCβ2.8} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}bsk07909 No P{PZ}bs03267 No insertion of mobile activating element P{EPgy2}bsEY23316 NA P{XP}bsd01376 NA P{XP}bsd04015 NA P{XP}bsd05493 NA P{XP}bsd05688 NA P{XP}bsd05976 NA insertion of enhancer trap binary system Mi{ET1}bsMB00417 No P{GAL4}bs1348 No miscellaneous insertions Mi{MIC}bsMI01549 NA P{CaryP}attP23 NA P{UAS-LUC.M}attP23 NA
Gene Ontology: Function, Process & Cellular Component ( 14 unique terms )
Terms Based on Experimental Evidence ( 10 terms )
Molecular Function
CV term Evidence References
activating transcription factor binding inferred from physical interaction with Mrtf (Han et al., 2004) contributes_to RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription inferred from physical interaction with Mrtf (Han et al., 2004)
Biological Process
CV term Evidence References
apposition of dorsal and ventral imaginal disc-derived wing surfaces inferred from mutant phenotype (Prout et al., 1997) imaginal disc-derived wing morphogenesis inferred from mutant phenotype (Dworkin and Gibson, 2006) imaginal disc-derived wing vein morphogenesis inferred from mutant phenotype (Han et al., 2004) open tracheal system development inferred from expression pattern (Affolter et al., 1994) positive regulation of transcription from RNA polymerase II promoter inferred from direct assay (Han et al., 2004) sleep inferred from mutant phenotype (Donlea et al., 2009) terminal branching, open tracheal system inferred from mutant phenotype (Guillemin and Krasnow, 1995, Guillemin et al., 1996)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Affolter et al., 1994, Somogyi and Rorth, 2004)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from electronic annotation with InterPro:IPR002100 (FlyBase Curators et al., 2004-) protein dimerization activity inferred from electronic annotation with InterPro:IPR002100 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
cell fate commitment traceable author statement (Milan and Cohen, 2000) imaginal disc-derived wing morphogenesis non-traceable author statement (Tyler and Baker, 2003) traceable author statement (Milan and Cohen, 2000, Crozatier et al., 2004, de Celis, 2003) open tracheal system development non-traceable author statement (Swiss-Prot Project Members, 1998.2.1) traceable author statement (Wilson and Leptin, 2000) terminal branching, open tracheal system non-traceable author statement (Zelzer and Shilo, 2000) traceable author statement (Montagne et al., 1996, Ghabrial et al., 2003) terminal cell fate specification, open tracheal system traceable author statement (Affolter and Shilo, 2000, Uv et al., 2003, Zelzer and Shilo, 2000)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Swiss-Prot Project Members, 1998.2.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions bs allele Gene References bs1 csw (Firth et al., 2000) bs2 brm (Marenda et al., 2004) csw (Firth et al., 2000) emc (Baonza and Garcia-Bellido, 1999) if (Wessendorf et al., 1992) LIMK1 (Chen et al., 2004) rho (Sturtevant and Bier, 1995, Roch et al., 1998) Snr1 (Marenda et al., 2004) bs2R-19 mys (Prout et al., 1997) bs11 rho (Fristrom et al., 1994, Sturtevant and Bier, 1995) bs12 rho (Fristrom et al., 1994) bs211 Mer (LaJeunesse et al., 2001) bs221 Mer (LaJeunesse et al., 2001) bs237 Mer (LaJeunesse et al., 2001) bs242 sbb (LaJeunesse et al., 2001) bs03267 br (Ward et al., 2003) brm (Marenda et al., 2004) csw (Firth et al., 2000) Dl (Roch et al., 1998) Egfr (Roch et al., 1998) emc (Roch et al., 1998, Baonza and Garcia-Bellido, 1999) H (Johannes and Preiss, 2002) Mer (LaJeunesse et al., 2001) N (Roch et al., 1998) px (Roch et al., 1998, Matakatsu et al., 1999) rho (Roch et al., 1998) rl (Roch et al., 1998) Snr1 (Marenda et al., 2004) vn (Roch et al., 1998) bsA48 rho (Fristrom et al., 1994) bsba rho (Sturtevant and Bier, 1995) bsEbr292 br (Ward et al., 2003) bsF31 Dl (Klein and Campos-Ortega, 1992) bsk07909 br (Ward et al., 2003) csw (Firth et al., 2000) Ras85D (Schnorr et al., 2001) Snr1 (Marenda et al., 2004) bsPx rho (Sturtevant and Bier, 1995) bsunspecified mys (Wessendorf et al., 1992, Fristrom et al., 1994, Prout et al., 1997) bsV100 if (Walsh and Brown, 1998) mys (Walsh and Brown, 1998) bsV121 if (Walsh and Brown, 1998) mew (Walsh and Brown, 1998) unspecified aya (Suzuki et al., 2000) Mer (LaJeunesse et al., 2001) mys (Santamaria and Randsholt, 1995) rho (Montagne et al., 1996) Tm1 (Ward et al., 2003) vn (Montagne et al., 1996)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63464 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004101 DroID - A comprehensive database of gene and protein interactions. • FBgn0004101 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37890
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11392 Dere\GG22961 Dgri\GH20989 Dmoj\GI21322 Dper\GL20046 Dpse\GA17433 Dsec\GM11854 Dsim\GD11852 Dvir\GJ21595 Dwil\GK22205 Dyak\GE14398
Curated drosophilid orthologs	Dpse\GA17433
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • XP_001120126.1 Apis mellifera (honey bee) 65714 Branchiostoma floridae (lancelet) 90266 Branchiostoma floridae (lancelet) ENSBTAP00000016981 Bos taurus (bovine) ENSCAFP00000002675 Canis familiaris (dog) JA15646 Caenorhabditis japonica (nematode) DDB0214892 Dictyostelium discoideum (amoeba) ENSDARP00000090012 Danio rerio (zebra fish) ENSGACP00000004953 Gymnopilus aculeatus (mushroom) ENSP00000265354 Homo sapiens (human) ENSMODP00000023686 Monodelphis domestica (gray opossum) ENSMMUP00000033647 Macaca mulatta (rhesus monkey) ENSMUSP00000015749 Mus musculus (house mouse) PHUM000573-PA Pediculus humanus (human body louse) ENSPPYP00000018605 Pongo pygmaeus (orangutan) ENSPTRP00000031085 Pan troglodytes (chimpanzee) ENSRNOP00000058149 Rattus norvegicus (norway rat) 4838520 Sorghum bicolor (sorghum) XP_001190796.1 Strongylocentrotus purpuratus (purple sea urchin) 59813 Trichoplax adhaerens (metazoa) XP_972565.2 Tribolium castaneum (red flour beetle) ENSTNIP00000005490 Tetraodon nigroviridis (green spotted pufferfish) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV18787 LH24098 ISCW019966 FBgn0232006 FBgn0224190 FBgn0183591 FBgn0166795 JGI_V11_8703 FBgn0077446 FBgn0157643 FBgn0128451 FBgn0115118 FBgn0088432 CPIJ008335 BGIBMGA001384 ACYPI002973 GB15081 AGAP010512 AAEL010926 AAEL008720 PB22587 PHUM516920 GLEAN_04911
Stocks & Reagents
Stocks Listed in FlyBase ( 21 )
Bloomington	378 px1 bs3 403 sp2 bs2 246 bw1 bsba 6354 w*; P{GAL4}bs1348
Harvard	- P{XP}bsd01376 - P{XP}bsd05493
Kyoto	105880 px1 bs3 105894 sp2 bs2 105813 bw1 bsba 101834 w1 ; bs4 / CyO 108750 w*; P{GAL4}bs1348
VDRC	v100609 P{KK108659}VIE-260B
	More stocks available...
Genomic Clones ( 1 )
	BACR02A05 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 308 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE17834
Other clones	DPiM_RE17834
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH17272 GH18414 GM21379 LD14764 LP10204 LP15168 RE05714 RE35519 RE37645 RE44870 RE47606 RE55982 RE61753 RH04324 RH04390 RH07193 RH10576 RH10904 RH11852 RH12090 RH13479 RH13709 RH16019 RH16209 RH17093 RH17215 RH17293 RH17409 RH18095 RH20543 RH21653 RH21844 RH22177 RH22556 RH22735 RH23477 RH26342 RH26817 RH26972 RH29568 RH29611 RH29690 RH29717 RH30058 RH30182 RH30333 RH30767 RH31611 RH31616 RH32493 RH33778 RH37622 RH38076 RH39930 RH42014 RH42077 RH42543 RH42985 RH44978 RH45176 RH46016 RH46595 RH46639 RH47060 RH47229 RH47315 RH47775 RH48091 RH49746 RH50730 RH51215 RH51705 RH51995 RH52448 RH52603 RH54383 RH54653 RH55168 RH55173 RH55446 RH55941 RH56741 RH56809 RH56819 RH56839 RH56978 RH57786 RH58019 RH58044 RH58239 RH59131 RH60268 RH60713 RH61408 RH61433 RH61508 RH61691 RH62378 RH63266 RH64377 RH65659 RH66252 RH66273 RH66292 RH67049 RH67365 RH67395 RH67957 RH68761 RH68859 RH69793 RH71145 RH73083 RH74330 SD02265 SD16292 SD18363 SD23611 SD25304
Other clones	11693 12697 53035 53115 80719 101359 193386 214349.60 AI124308 CK00330 CK00365 CK01023 CK01258 CK01331 CK01336 CK01363 CK01421 CK01448 CK01456 CK01603 CK01617 CK01634 CK01718 CK01792 CK01833 CK01958 CK02010 CK02026 CK02083 CK02089 CK02102 CK02134 CK02139 CK02216 CK02225 CK02311 CK02315 CK02322 CK02429 CK02526 CK02564 CK02584 CK02648 EC41454 EC053545 EK027108 EK030002 EK031312 EK032025 EK034826 EK043120 EK047843 EK048304 EK049563 EK050409 EK053809 EK058252 EK058646 EK059016 EK072843 EK073259 EK074745 EK075111 EK077604 EK082259 EK085812 EK087859 EK089024 EK089958 EK092228 EK092504 EK095603 EK104936 EK105342 EK109250 EK112136 EK113540 EK113756 EK115509 EK116527 EK117157 EK117938 EK119258 EK121312 EK129929 EK130147 EK133510 EK139758 EK145429 EK147049 EK150160 EK154064 EK159162 EK159214 EK160129 EK161453 EK165022 EK165624 EK165964 EK170013 EK170051 EK178259 EK182949 EK183856 EK183952 EK184711 EK187045 EK194453 EK194822 EK197709 EK198161 EK198534 EK201106 EK203155 EK205033 EK206320 EK207149 EK209011 EK213827 EK215732 EK217506 EK219351 EK221844 EK225650 EK225919 EK227244 EK228443 EK240954 EK241147 EK243327 EK246762 EK253707 EK258226 EK261906 EK262115 EK265613 EK267119 EK267342 EK267760 EK278350 EK278462 EK289455 EK289458 EK290364 EK291554 EK292041 EK296210 EK301716 EK303642 EK304364 EN01164 EN02064 EN15045 EP00315 EP01012 EP02341 EP04728 EP04754 EP05547 EP07649 EP08720 EP08834 EP08943 EP09905 EP10234 EP10464 EP10540 EP11446 EP11516 EP11550 EP11622 EP11914 EP13133 EP13602 EP14132 EP14842 EP16650 EP17315 EP17909 EP18944 EP19157 EP19847 EP21045 RP003300615 RP003310238 RP003315002 RP003339172
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0004101 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37890
Antibody Information
	polyclonal (Affolter et al., 1994)
Other Information
Discoverer
	Bridges, 16th Nov. 1911.
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: bs CG3411 (Wang, 1999.11)
Source for database merge of
Additional comments	May be part of a pseudoallelic complex with Px.
Other Comments
	RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) bs is involved in cell fate allocation and differentiation but does not participate in cell proliferation. The activity of bs is necessary to specify cells to adopt an intervein fate. It expression is repressed in larval stages in vein territories by genes of the Ras signalling pathway. The differentiation of vein and intervein depends on the outcome of a balance between bs and rho activities, achieved during pupal stages. (Roch et al., 1998) bs regulates formation of the terminal branches of the tracheal system. bs may function like mammalian SRF in an inducible transcription complex. Activation of the complex by signals from target tissues induces expression of genes involved in cytoplasmic outgrowth. (Guillemin et al., 1996) The bs gene product plays a dual role during wing differentiation. It acts in a dosage-dependent manner to suppress the formation of wing veins and is required cell-autonomously to promote the development of intervein cells. bs acts at a key step between regulatory genes that define the early positional values in the developing wing disc and the subsequent localized expression of intervein-specific structural genes. (Montagne et al., 1996) Required for the formation and outgrowth of the terminal branches of the tracheal system. (Guillemin and Krasnow, 1995) The bs gene might play a role in the proper formation and maintenance of the trachea. (Affolter et al., 1994) Developmental and genetic analyses suggest that the bs product is required during metamorphosis for the initiation of intervein development and the concommitant inhibition of vein development. (Fristrom et al., 1994) bs may affect dorsal-ventral flattening of the wing during prepupal morphogenesis. (Gotwals and Fristrom, 1991)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_166667 NM_001144279 RefSeq (Proteins) • NP_726438 NP_001137751 DDBJ / EMBL / GenBank • AAF47195 AAM11399 ACL83204 AY095071 BI212701 CAA54670 CZ472194 CZ473688 CZ474566 CZ474850 CZ474851 G00561 S81937 X77532 Entrez Gene - A searchable database of RefSeq genes. • 37890 UniProtKB/Swiss-Prot • Q24535
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Transcription factor, MADS-box (IPR002100) Armadillo-like helical (IPR011989)
Linkouts
	BioGRID - A database of protein and genetic interactions • 63464 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0004101 DroID - A comprehensive database of gene and protein interactions. • FBgn0004101 DRSC - Results from RNAi screens. • FBgn0004101 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004101 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3411-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004101 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37890 InParanoid A subset of ortholog calls from InParanoid. • XP_001120126.1 Apis mellifera (honey bee) 65714 Branchiostoma floridae (lancelet) 90266 Branchiostoma floridae (lancelet) ENSBTAP00000016981 Bos taurus (bovine) ENSCAFP00000002675 Canis familiaris (dog) JA15646 Caenorhabditis japonica (nematode) DDB0214892 Dictyostelium discoideum (amoeba) ENSDARP00000090012 Danio rerio (zebra fish) ENSGACP00000004953 Gymnopilus aculeatus (mushroom) ENSP00000265354 Homo sapiens (human) ENSMODP00000023686 Monodelphis domestica (gray opossum) ENSMMUP00000033647 Macaca mulatta (rhesus monkey) ENSMUSP00000015749 Mus musculus (house mouse) PHUM000573-PA Pediculus humanus (human body louse) ENSPPYP00000018605 Pongo pygmaeus (orangutan) ENSPTRP00000031085 Pan troglodytes (chimpanzee) ENSRNOP00000058149 Rattus norvegicus (norway rat) 4838520 Sorghum bicolor (sorghum) XP_001190796.1 Strongylocentrotus purpuratus (purple sea urchin) 59813 Trichoplax adhaerens (metazoa) XP_972565.2 Tribolium castaneum (red flour beetle) ENSTNIP00000005490 Tetraodon nigroviridis (green spotted pufferfish) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /newgene/serumrf1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37890 modMine - Data generated by the modENCODE project. • FBgn0004101 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV18787 LH24098 ISCW019966 FBgn0232006 FBgn0224190 FBgn0183591 FBgn0166795 JGI_V11_8703 FBgn0077446 FBgn0157643 FBgn0128451 FBgn0115118 FBgn0088432 CPIJ008335 BGIBMGA001384 ACYPI002973 GB15081 AGAP010512 AAEL010926 AAEL008720 PB22587 PHUM516920 GLEAN_04911 REDfly - A database of transcriptional regulatory elements. • FBgn0004101
Synonyms & Secondary IDs ( 32 )
Reported As
Symbol Synonym	ba (Gridley et al., 1990, Thompson, 1963) BS (Crozatier et al., 2002) bs (Janody and Treisman, 2006, Sotillos and De Celis, 2005, Bokel et al., 2005, Curtis et al., 2011, Stahl et al., 2007, Chen et al., 2005, Markstein et al., 2008, Centanin et al., 2008, Lyulcheva et al., 2008, Barrio et al., 2007, Cabernard and Affolter, 2005, Christensen et al., 2009.5.6, Donlea et al., 2009, Angulo et al., 2004, Terriente-Félix and de Celis, 2009, Palsson et al., 2005, Thompson, 2010, Rendina et al., 2010, Beltran et al., 2007, Egoz-Matia et al., 2011) Bs (Gomez et al., 2005, Molnar et al., 2006, Christoforou et al., 2008, Molnar et al., 2007, Terriente-Félix et al., 2010, Dworkin et al., 2011) bs/DSRF (Dworkin and Gibson., 2006, Dworkin and Gibson, 2006) bs/Dsrf (Jung et al., 2006) CG3411 (Perkins et al., 2009.3.31, Keleman et al., 2009.8.5) DmSRF (Avila et al., 2002) DSRF (Shimmi et al., 2005, Kwon et al., 2005, Ralston and Blair, 2005, Matakatsu and Blair, 2004, Lundstrom et al., 2004, Uv et al., 2003, Hemphala et al., 2003, Jazwinska and Affolter, 2003, Englund et al., 2002, Shim et al., 2001, Dossenbach et al., 2001, Halder and Carroll, 2001, Hemavathy et al., 2000, Conley et al., 2000, Steneberg et al., 1999, Imam et al., 1999, Vincent et al., 1998, Weatherbee et al., 1998, Steneberg et al., 1998, Manning et al., 1998, Hacohen et al., 1998, Blair and Ralston, 1997, Ryan et al., 1997, Lee et al., 1996, Montagne et al., 1995, Affolter et al., 1994, Affolter et al., 1994, Jung et al., 2006, Jiang and Crews, 2006, Cela and Llimargas, 2006, Petit et al., 2004, Serpe et al., 2008, Araujo et al., 2007, O'Keefe et al., 2009, Grieder et al., 2009, Gervais and Casanova, 2010, Wagner et al., 2010, Usha and Shashidhara, 2010, Egoz-Matia et al., 2011, Dworkin et al., 2011, Liu et al., 2011) D-SRF (Crozatier et al., 2004, Crozatier et al., 2002, Vervoort, 2002, Dubois and Vincent, 2001, Maschat et al., 2001, Vervoort et al., 1999) dSRF (Milan et al., 2002, Milan and Cohen, 2000, Treisman, 1996, Takaesu et al., 2006, Moon et al., 2005, Evans et al., 2009) dsrf (Weatherbee and Carroll, 1999, O'Keefe et al., 2007) DSrf (Englund et al., 1999) DSrf/bs (Affolter and Shilo, 2000) Group IIc (LaJeunesse et al., 2001) l(2)03267   Mal (Thompson, 2010) pruned/DSRF (Guillemin, 1996.7.31, Sutherland et al., 1996) px (Plough and Ives, 1934) Serf   SRF (Han et al., 2004, Kato et al., 2004, Somogyi and Rorth, 2004, Dorfman et al., 2002, Cripps and Olson, 2002, Chen et al., 2002, Wilson and Leptin, 2000, Chihara and Hayashi, 2000, Grenier and Carroll, 2000, Placzek and Skaer, 1999, Gibson, 1999, Halder et al., 1998, Nguyen and Frasch, 2006, Takaesu et al., 2005, Thompson, 2010) Srf (Weatherbee and Carroll, 1999, )
Name Synonym	balloon (Gridley et al., 1990) blistered (Pipes et al., 2006, Dworkin and Gibson, 2006, Sotillos and De Celis, 2005, Chen et al., 2005, Lyulcheva et al., 2008, Araujo et al., 2007, Barrio et al., 2007, Donlea et al., 2009, Thompson, 2010, Rendina et al., 2010, Gambis et al., 2011) Blistered (Takaesu et al., 2005, Molnar et al., 2007) blistered/Drosophila serum response factor (Jung et al., 2006) Drosophila serum response factor (Jiang and Crews, 2006) Drosophila Serum Response Factor (Petit et al., 2004, O'Keefe et al., 2007) pruned (Wilk et al., 2000, Boube and Vincent, 1997, Guillemin et al., 1997, Uv et al., 2003, Placzek and Skaer, 1999, Englund et al., 1999, Guillemin et al., 1996, Guillemin and Krasnow, 1995, ) serum response factor (Chen et al., 2002, Wilson and Leptin, 2000, Nguyen and Frasch, 2006) Serum Response Factor (Groppe, 1994.2.3, Liu et al., 2011) Serum response factor (Takaesu et al., 2005)
Secondary FlyBase IDs
	FBgn0000156 FBgn0000223 FBgn0010354 FBgn0010535
References ( 228 )
Generate a list of	All references relating to this gene ( 228 )
List References by type	Research paper  ( 138 ) Supplementary material  ( 3 ) Review  ( 32 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 28 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Curated genome annotation  ( 2 ) Computer file  ( 4 ) Protein sequence record  ( 1 ) Book  ( 3 ) Thesis  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 11 )
	Curtis et al., 2011, Dev. Biol. 350(2): 534--547 Histone lysine demethylases function as co-repressors of SWI/SNF remodeling activities during Drosophila wing development. [FBrf0212876] Dworkin et al., 2011, Genetics 187(4): 1171--1184 The effects of weak genetic perturbations on the transcriptome of the wing imaginal disc and its association with wing shape in Drosophila melanogaster. [FBrf0214376] Egoz-Matia et al., 2011, Dev. Biol. 351(1): 99--109 Spatial regulation of cell adhesion in the Drosophila wing is mediated by Delilah, a potent activator of βPS integrin expression. [FBrf0213026] Gambis et al., 2011, Dev. Biol. 351(1): 128--134 Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila. [FBrf0213000] Liu et al., 2011, Dev. Growth Differ. 53(6): 822--841 Negative modulation of bone morphogenetic protein signaling by Dullard during wing vein formation in Drosophila. [FBrf0214664] Gervais and Casanova, 2010, Curr. Biol. 20(4): 359--366 In Vivo Coupling of Cell Elongation and Lumen Formation in a Single Cell. [FBrf0210105] Rendina et al., 2010, Genetics 186(1): 167--181 Bap170, a Subunit of the Drosophila PBAP Chromatin Remodeling Complex, Negatively Regulates the EGFR Signaling. [FBrf0211845] Terriente-Félix et al., 2010, Genetics 185(2): 671--684 Identification of genes affecting wing patterning through a loss-of-function mutagenesis screen and characterization of med15 function during wing development. [FBrf0211230] Thompson, 2010, PLoS ONE 5(4): e10077 Mal/SRF is dispensable for cell proliferation in Drosophila. [FBrf0210573] Usha and Shashidhara, 2010, Dev. Biol. 341(2): 389--399 Interaction between Ataxin-2 Binding Protein 1 and Cubitus-interruptus during wing development in Drosophila. [FBrf0210647] Wagner et al., 2010, Dev. Biol. 339(1): 1--13 The Drosophila LEM-domain protein MAN1 antagonizes BMP signaling at the neuromuscular junction and the wing crossveins. [FBrf0210050] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...