FB2021_03, released June 15, 2021

Gene: Dmel\blow

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General Information
Symbol
Dmel\blow
Species
D. melanogaster
Name
blown fuse
Annotation Symbol
CG1363
Feature Type
protein_coding_gene
FlyBase ID
FBgn0004133
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Summary
blown fuse (blow) encodes a cytoplasmic protein required for myoblast fusion. It regulates actin cytoskeletal dynamics via modulating the stability of the WASp-Vrp1 complex. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

l(2)43Eb

Key Links
Genomic Location
Cytogenetic map
43E1-43E3
Sequence location
2R:7,580,488..7,587,079 [-]
Recombination map
2-57
RefSeq locus
NT_033778 REGION:7580488..7587079
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with FLYBASE:rols; FB:FBgn0041096
(Kreiskother et al., 2006)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in actin cytoskeleton organization
inferred from mutant phenotype
(Richardson et al., 2007)
involved_in mesoderm development
inferred from expression pattern
(Furlong et al., 2001)
involved_in myoblast fusion
inferred from mutant phenotype
(Bulchand et al., 2010, Richardson et al., 2007, Estrada et al., 2006, Menon et al., 2005, Schroter et al., 2004, Doberstein et al., 1997)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in myoblast fusion
traceable author statement
(Furlong et al., 2001)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Doberstein et al., 1997)
located_in podosome
inferred from direct assay
(Jin et al., 2011)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
blown fuse (blow) encodes a cytoplasmic protein required for myoblast fusion. It regulates actin cytoskeletal dynamics via modulating the stability of the WASp-Vrp1 complex. [Date last reviewed: 2019-03-07]
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\blow for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.42

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
blow-RA
FBtr0088952
NM_165542
2632
644
blow-RB
FBtr0300368
NM_001169603
2270
637
blow-RD
FBtr0310050
NM_001169602
2612
644
Additional Transcript Data and Comments
Reported size (kB)

2.6 (northern blot)

(Doberstein et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
blow-PA
FBpp0088026
69.4
644
6.33
NP_724580
AAF59227
blow-PB
FBpp0289597
68.7
637
6.46
NP_001163074
ACZ94353
blow-PD
FBpp0301751
69.4
644
6.33
NP_001163073
ACZ94352
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

644 aa isoforms: blow-PA, blow-PD
Additional Polypeptide Data and Comments
Reported size (kDa)

644 (aa); 70 (kD observed)

(Doberstein et al., 1997)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\blow using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 10
mesoderm | restricted | segmentally repeated
(Doberstein et al., 1997)
embryonic stage 11
mesoderm
(Cunha et al., 2010)
embryonic stage 11 -- 12
circular visceral muscle primordium
(Fisher et al., 2012)
somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
embryonic stage 11 -- 14
embryonic myoblast
(Doberstein et al., 1997)
embryonic stage 13 -- 16
embryonic primordium of adult muscle precursor
(Fisher et al., 2012)
dorsal pharyngeal muscle
(Fisher et al., 2012)
embryonic/larval muscle system
(Fisher et al., 2012)
embryonic/larval somatic muscle
(Fisher et al., 2012)
embryonic/larval visceral muscle
(Fisher et al., 2012)
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
pupal stage P6
dorsal medial indirect flight muscle

Comment: 24 hr APF

(Bryantsev et al., 2012)
thoracic adult muscle precursor cell
(Bryantsev et al., 2012)
Additional Descriptive Data

blow transcripts are expressed in myoblasts just before and during myoblast fusion. Expression is first detected in late stage 10 embryos in 13 clusters of mesodermal cells, which may correspond to myoblast precursors. During stage 11 and 12, segmental stripes of fusing myoblasts are seen at the sites of the clusters. Expression peaks at early stage 13 and disappears by the end of stage 14.

(Doberstein et al., 1997)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 11 -- 14
embryonic myoblast
(Doberstein et al., 1997)
embryonic stage 14
fusion competent cell
(Jin et al., 2011)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data

blow protein expression is specific to fusion competent cells, and is not expressed in muscle founder cells.

(Jin et al., 2011)

blow protein is detected in unfused myoblasts at stages 11-14, with minor amounts of residual protein in early myotubes.

(Doberstein et al., 1997)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Doberstein et al., 1997)
located_in podosome
inferred from direct assay
(Jin et al., 2011)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\blow in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB
EMBL-EBI Single Cell Expression Atlas
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 6 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of blow
Transgenic constructs containing regulatory region of blow
Aberrations (Deficiencies and Duplications) ( 43 )
Inferred from experimentation ( 43 )
Gene disrupted in
Df(2L)88/07
(Doberstein et al., 1997)
Df(2R)cn-S5
(Heitzler et al., 1993)
Df(2R)cn-S6
(Heitzler et al., 1993)
Df(2R)cn-S8
(Roote, 1996.8, Heitzler et al., 1993)
Df(2R)Drlrv5
(Heitzler et al., 1993)
Df(2R)Drlrv7
(Heitzler et al., 1993)
Df(2R)Drlrv15
(Heitzler et al., 1993)
Df(2R)Drlrv18
(Heitzler et al., 1993)
Df(2R)Drlrv21
(Heitzler et al., 1993)
Df(2R)Drlrv24
(Heitzler et al., 1993)
Df(2R)Drlrv26
(Heitzler et al., 1993)
Df(2R)Drlrv30
(Heitzler et al., 1993)
Df(2R)Exel6052
(Ryder, 2004.4.26)
Df(2R)Exel6053
(Kahsai and Cook, 2018, Ryder, 2004.4.26)
Df(2R)NCX9
(Heitzler et al., 1993)
Df(2R)NCX11
(Heitzler et al., 1993)
Df(2R)NCX13
(Eeken et al., 2001)
Df(2R)Npk-rv1
(Roote and Ashburner, 2005.6)
Df(2R)sple-N3
(Heitzler et al., 1993)
Df(2R)ST1
(Eeken et al., 2001)
Gene not disrupted in
Df(2R)cos-2
(Heitzler et al., 1993)
Df(2R)cos-3
(Heitzler et al., 1993)
Df(2R)Drlrv3
(Heitzler et al., 1993)
Df(2R)Drlrv17
(Heitzler et al., 1993)
Df(2R)Drlrv20
(Heitzler et al., 1993)
Df(2R)Drlrv22
(Heitzler et al., 1993)
Df(2R)Drlrv25
(Heitzler et al., 1993)
Df(2R)Drlrv28
(Heitzler et al., 1993)
Df(2R)DX1
(Heitzler et al., 1993)
Df(2R)DX14
(Heitzler et al., 1993)
Df(2R)EW60
(Heitzler et al., 1993)
Df(2R)H23
(Heitzler et al., 1993)
Df(2R)nap2
(Heitzler et al., 1993)
Df(2R)NCX5
(Heitzler et al., 1993)
Df(2R)NCX8
(Eeken et al., 2001, Heitzler et al., 1993)
Df(2R)NCX10
(Heitzler et al., 1993)
Df(2R)pk-N5
(Heitzler et al., 1993)
Df(2R)pwn5
(Heitzler et al., 1993)
Df(2R)sple-D1
(Heitzler et al., 1993)
Df(2R)sple-D2
(Heitzler et al., 1993)
Df(2R)sple-D3
(Roote, 1996.8, Heitzler et al., 1993)
Df(2R)sple-J1
(Heitzler et al., 1993)
Df(2R)sple-J2
(Heitzler et al., 1993)
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\CA3
1 of 15
Yes
No
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (0)
No records found.
Rattus norvegicus (Norway rat) (0)
No records found.
Xenopus tropicalis (Western clawed frog) (0)
No records found.
Danio rerio (Zebrafish) (0)
No records found.
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\F59A2.5
1 of 15
Yes
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919091K )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00005689
Drosophila simulans
Dsim\GD10237
Drosophila sechellia
Dsec\GM20776
Drosophila erecta
Dere\GG10729
Drosophila yakuba
Dyak\GE23805
Drosophila ananassae
Dana\GF11709
Drosophila pseudoobscura pseudoobscura
Dpse\GA12421
Drosophila persimilis
Dper\GL20103
Drosophila willistoni
Dwil\GK10711
Drosophila virilis
Dvir\GJ20268
Drosophila mojavensis
Dmoj\GI19201
Drosophila grimshawi
Dgri\GH20021
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150A73 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17024197
Glossina morsitans
Tsetse fly
Gmor\GMOY001445
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_12224
Mayetiola destructor
Hessian fly
Mdes\Mdes011270
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL001137
Anopheles gambiae
Malaria mosquito
Agam\AGAP002495
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ009993
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0NBB )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Tribolium castaneum
Red flour beetle
Tcas\TC014242
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (1)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CAH15
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser
    View in Interactions Browser
    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    blow - Crk
    anti tag coimmunoprecipitation, anti tag western blot
    (Jin et al., 2011)
    blow - Vrp1
    anti tag coimmunoprecipitation, anti tag western blot, western blot
    (Jin et al., 2011)
    blow - rols
    two hybrid
    (Kreiskother et al., 2006)
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement
    View in Interactions Browser
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    blow
    suppressible
    Hem
    (Schroter et al., 2004)
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Hem
    enhanceable
    blow
    (Schroter et al., 2004)
    siz
    enhanceable
    blow
    (Bulchand et al., 2010)
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-57
    Cytogenetic map
    43E1-43E3
    Sequence location
    2R:7,580,488..7,587,079 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    43E1-43E3
    Limits computationally determined from genome sequence between P{EP}CG1600EP398 and P{PZ}scra03427&P{EP}EP2179
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location

    2-57

    (FlyBase, 2016)

    2-56.7

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (8)
    Genomic Clones (18)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (45)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     

    polyclonal

    (Jin et al., 2011, Doberstein et al., 1997)
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: blow CG1363

    (Gabrielian, 1999.11)
    Source for database merge of
    Additional comments
    Other Comments

    Gene expression is increased in response to the presence of two copies of Scer\GAL4hs.PB.

    (Liu and Lehmann, 2008)

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    (Kiger et al., 2003)

    blow is cloned and the expression pattern studied. Light and electron microscopy are used to study the process of myoblast fusion in blow mutants.

    (Doberstein et al., 1997)

    blow is essential for myoblast fusion.

    (Doberstein et al., 1997)

    The blow gene is required for myoblast fusion.

    (Doberstein, 1996.1.23)
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 47 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    DRscDB
    EMBL-EBI Single Cell Expression Atlas
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    MARRVEL_MODEL
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (8)
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (93)