FB2025_02 , released April 17, 2025
Gene: Dmel\blow
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General Information
Symbol
Dmel\blow
Species
D. melanogaster
Name
blown fuse
Annotation Symbol
CG1363
Feature Type
protein_coding_gene
FlyBase ID
FBgn0004133
Gene Model Status
Current
Stock Availability
9 publicly available
Gene Summary
blown fuse (blow) encodes a cytoplasmic protein required for myoblast fusion. It regulates actin cytoskeletal dynamics via modulating the stability of the WASp-Vrp1 complex. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

l(2)43Eb

Key Links
Genomic Location
Cytogenetic map
43E1-43E3
Sequence location
Recombination map
2-57
RefSeq locus
NT_033778 REGION:7580488..7587079
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with FLYBASE:rols; FB:FBgn0041096
(Kreiskother et al., 2006)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in actin cytoskeleton organization
inferred from mutant phenotype
(Richardson et al., 2007)
involved_in mesoderm development
inferred from expression pattern
(Furlong et al., 2001)
involved_in myoblast fusion
inferred from mutant phenotype
(Bulchand et al., 2010, Richardson et al., 2007, Estrada et al., 2006, Menon et al., 2005, Schroter et al., 2004, Doberstein et al., 1997)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in myoblast fusion
traceable author statement
(Furlong et al., 2001)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Doberstein et al., 1997)
located_in podosome
inferred from direct assay
(Jin et al., 2011)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
blown fuse (blow) encodes a cytoplasmic protein required for myoblast fusion. It regulates actin cytoskeletal dynamics via modulating the stability of the WASp-Vrp1 complex. [Date last reviewed: 2019-03-07]
Summary (Interactive Fly)

a cytoplasmic protein essential for somatic myoblast fusion - functions in stretching and outgrowth of circular visceral muscles - required for myoblast fusion - regulates actin cytoskeletal dynamics via modulating the stability of the WASp-Vrp1 complex.

Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\blow for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry A1Z714)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.42

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.48

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
blow-RA
FBtr0088952
NM_165542
2632
644
blow-RB
FBtr0300368
NM_001169603
2270
637
blow-RD
FBtr0310050
NM_001169602
2612
644
Additional Transcript Data and Comments
Reported size (kB)

2.6 (northern blot)

(Doberstein et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
blow-PA
FBpp0088026
69.4
644
6.33
A1Z714
NP_724580
AAF59227
blow-PB
FBpp0289597
68.7
637
6.46
E1JGZ4
NP_001163074
ACZ94353
blow-PD
FBpp0301751
69.4
644
6.33
A1Z714
NP_001163073
ACZ94352
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

644 aa isoforms: blow-PA, blow-PD
Additional Polypeptide Data and Comments
Reported size (kDa)

644 (aa); 70 (kD observed)

(Doberstein et al., 1997)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\blow using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.33

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 10
mesoderm | restricted | segmentally repeated
(Doberstein et al., 1997)
embryonic stage 11
mesoderm
(Cunha et al., 2010)
embryonic stage 11 -- 12
circular trunk visceral muscle primordium
(Fisher et al., 2012)
somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
trunk visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
embryonic stage 11 -- 14
embryonic myoblast
(Doberstein et al., 1997)
embryonic stage 13 -- 16
embryonic primordium of adult muscle precursor
(Fisher et al., 2012)
dorsal pharyngeal muscle cell
(Fisher et al., 2012)
larval muscle system
(Fisher et al., 2012)
larval somatic muscle cell
(Fisher et al., 2012)
larval visceral muscle cell
(Fisher et al., 2012)
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
pupal stage P6
dorsal longitudinal indirect flight muscle cell

Comment: 24 hr APF

(Bryantsev et al., 2012)
thoracic adult muscle precursor cell
(Bryantsev et al., 2012)
Additional Descriptive Data

blow transcripts are expressed in myoblasts just before and during myoblast fusion. Expression is first detected in late stage 10 embryos in 13 clusters of mesodermal cells, which may correspond to myoblast precursors. During stage 11 and 12, segmental stripes of fusing myoblasts are seen at the sites of the clusters. Expression peaks at early stage 13 and disappears by the end of stage 14.

(Doberstein et al., 1997)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 11 -- 14
embryonic myoblast
(Doberstein et al., 1997)
embryonic stage 14
fusion competent cell
(Jin et al., 2011)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data

blow protein expression is specific to fusion competent cells, and is not expressed in muscle founder cells.

(Jin et al., 2011)

blow protein is detected in unfused myoblasts at stages 11-14, with minor amounts of residual protein in early myotubes.

(Doberstein et al., 1997)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Doberstein et al., 1997)
located_in podosome
inferred from direct assay
(Jin et al., 2011)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\blow in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 6 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of blow
Transgenic constructs containing regulatory region of blow
Aberrations (Deficiencies and Duplications) ( 43 )
Inferred from experimentation ( 43 )
Gene disrupted in
Df(2L)88/07
(Doberstein et al., 1997)
Df(2R)cn-S5
(Heitzler et al., 1993)
Df(2R)cn-S6
(Heitzler et al., 1993)
Df(2R)cn-S8
(Roote, 1996.8, Heitzler et al., 1993)
Df(2R)Drlrv5
(Heitzler et al., 1993)
Df(2R)Drlrv7
(Heitzler et al., 1993)
Df(2R)Drlrv15
(Heitzler et al., 1993)
Df(2R)Drlrv18
(Heitzler et al., 1993)
Df(2R)Drlrv21
(Heitzler et al., 1993)
Df(2R)Drlrv24
(Heitzler et al., 1993)
Df(2R)Drlrv26
(Heitzler et al., 1993)
Df(2R)Drlrv30
(Heitzler et al., 1993)
Df(2R)Exel6052
(Ryder, 2004.4.26)
Df(2R)Exel6053
(Kahsai and Cook, 2018, Ryder, 2004.4.26)
Df(2R)NCX9
(Heitzler et al., 1993)
Df(2R)NCX11
(Heitzler et al., 1993)
Df(2R)NCX13
(Eeken et al., 2001)
Df(2R)Npk-rv1
(Roote and Ashburner, 2005.6)
Df(2R)sple-N3
(Heitzler et al., 1993)
Df(2R)ST1
(Eeken et al., 2001)
Gene not disrupted in
Df(2R)cos-2
(Heitzler et al., 1993)
Df(2R)cos-3
(Heitzler et al., 1993)
Df(2R)Drlrv3
(Heitzler et al., 1993)
Df(2R)Drlrv17
(Heitzler et al., 1993)
Df(2R)Drlrv20
(Heitzler et al., 1993)
Df(2R)Drlrv22
(Heitzler et al., 1993)
Df(2R)Drlrv25
(Heitzler et al., 1993)
Df(2R)Drlrv28
(Heitzler et al., 1993)
Df(2R)DX1
(Heitzler et al., 1993)
Df(2R)DX14
(Heitzler et al., 1993)
Df(2R)EW60
(Heitzler et al., 1993)
Df(2R)H23
(Heitzler et al., 1993)
Df(2R)nap2
(Heitzler et al., 1993)
Df(2R)NCX5
(Heitzler et al., 1993)
Df(2R)NCX8
(Eeken et al., 2001, Heitzler et al., 1993)
Df(2R)NCX10
(Heitzler et al., 1993)
Df(2R)pk-N5
(Heitzler et al., 1993)
Df(2R)pwn5
(Heitzler et al., 1993)
Df(2R)sple-D1
(Heitzler et al., 1993)
Df(2R)sple-D2
(Heitzler et al., 1993)
Df(2R)sple-D3
(Roote, 1996.8, Heitzler et al., 1993)
Df(2R)sple-J1
(Heitzler et al., 1993)
Df(2R)sple-J2
(Heitzler et al., 1993)
Inferred from location ( 5 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (0)
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (0)
Mus musculus (laboratory mouse) (0)
Xenopus tropicalis (Western clawed frog) (0)
Danio rerio (Zebrafish) (0)
Caenorhabditis elegans (Nematode, roundworm) (0)
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP002495
6 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:blow. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    Interaction Browsers
    View in FlyBase Interactions Browser   View in MIST tool (external link)
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    blow
    suppressible
    Hem
    (Schroter et al., 2004)
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Hem
    enhanceable
    blow
    (Schroter et al., 2004)
    Iqsec
    enhanceable
    blow
    (Bulchand et al., 2010)
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyBase
    External Links
    External Data
    Linkouts
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-57
    Cytogenetic map
    43E1-43E3
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    43E1-43E3
    Limits computationally determined from genome sequence between P{EP}CG1600EP398 and P{PZ}scra03427&P{EP}EP2179
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location

    2-57

    (FlyBase, 2016)

    2-56.7

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (9)
    Genomic Clones (18)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (36)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    Antibody Information
    Laboratory Generated Antibodies
     

    polyclonal

    (Jin et al., 2011, Doberstein et al., 1997)
    Commercially Available Antibodies
     
    Cell Line Information
    Publicly Available Cell Lines
     
      Other Stable Cell Lines
       
        Other Comments

        Gene expression is increased in response to the presence of two copies of Scer\GAL4hs.PB.

        (Liu and Lehmann, 2008)

        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

        (Kiger et al., 2003)

        blow is cloned and the expression pattern studied. Light and electron microscopy are used to study the process of myoblast fusion in blow mutants.

        (Doberstein et al., 1997)

        blow is essential for myoblast fusion.

        (Doberstein et al., 1997)

        The blow gene is required for myoblast fusion.

        (Doberstein, 1996.1.23)
        Relationship to Other Genes
        Source for database merge of
        Additional comments
        Nomenclature History
        Source for database identify of

        Source for identity of: blow CG1363

        (Gabrielian, 1999.11)
        Nomenclature comments
        Etymology
        Synonyms and Secondary IDs (9)
        Reported As
        Symbol Synonym
        BLOW
        (Dubey et al., 2020)
        Blow
        (Rout et al., 2022, Bothe and Baylies, 2016, Abmayr and Pavlath, 2012, Chen et al., 2007, Griemert et al., 2007, Kreiskother et al., 2006, Schroeter et al., 2006)
        CG1363
        (National Institute of Genetics Fly Stocks, 2023-, Ibrahim et al., 2013, Abmayr and Pavlath, 2012, Keleman et al., 2009.8.5, Rebeiz et al., 2002)
        blow
        (Collins et al., 2024, Trujillo et al., 2024, Lee and Chen, 2019, Wang et al., 2018, Dubois et al., 2016, Deng et al., 2015, Kwon et al., 2015, Rodal et al., 2015, Takahara and Takahashi, 2015, Wang et al., 2015, Bothe et al., 2014, Ciglar et al., 2014, Rudolf et al., 2014, Bonn et al., 2013, Kwon et al., 2013, Bryantsev et al., 2012, Busser et al., 2012, Dobi et al., 2011, Friedman et al., 2011, Hornbruch-Freitag et al., 2011, Jin et al., 2011, Bulchand et al., 2010, Cunha et al., 2010, Wasbrough et al., 2010, Gilchrist et al., 2008, Liu and Lehmann, 2008, Beckett and Baylies, 2007, Estrada et al., 2007, Kesper et al., 2007, Richardson et al., 2007, Robida et al., 2007, Wolfstetter et al., 2007, Estrada et al., 2006, Sandmann et al., 2006, Hossain et al., 2005, Menon et al., 2005)
        l(2)43Eb
        (Eeken et al., 2001, Roote, 1995.9.26)
        Name Synonyms
        Blown fuse
        (Wang et al., 2015, Jin et al., 2011, Chen et al., 2007)
        blown fuse
        (Ibrahim et al., 2013, Busser et al., 2012, Bulchand et al., 2010, Cunha et al., 2010, Estrada et al., 2007, Wolfstetter et al., 2007)
        blown-fuse
        (Dobi et al., 2011)
        blownfuse
        (Menon et al., 2005)
        Secondary FlyBase IDs
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          Study result (0)
          Result
          Result Type
          Title
          External Crossreferences and Linkouts ( 48 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
          FlyMine - An integrated database for Drosophila genomics
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MARRVEL_MODEL - MARRVEL (model organism gene)
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
          MIST (genetic) - An integrated Molecular Interaction Database
          MIST (protein-protein) - An integrated Molecular Interaction Database
          References (100)