Gene model reviewed during 5.52
There is only one protein coding transcript and one polypeptide associated with this gene
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nullo using the Feature Mapper tool.
nullo transcripts are first detected at embryonic cycle 11, peak during the mitosis separating cycles 13 and 14, and disappear rapidly at the beginning of cellularization. Expression occurs throughout the cortex of the embryo with less RNA accumulation at the poles than in the rest of the embryo. In cycle 14 broad bands of heavier accumulation are visible. Transcript levels in cycle 14 may be coupled to the nucleocytoplasmic ratio. nullo is not expressed at any other times in development. The onset of expression is not affected in Sry-α mutants and does not require any other zygotically acting genes.
nullo protein is blastoderm-specific and is detected between interphase of embryonic cycle 13 and the beginning of gastrulation, with highest levels in cycle 14 and during the slow phase of cellularization. nullo protein localizes between interphase actin caps and within metaphase furrows during cycle 13. At cycle 14, nullo protein co-localizes with the actin network in a punctate hexagonal pattern, and during the slow phase of cellularization continues to be associated with the plasma membrane at, and apical to, the invaginating furrow canal. During the fast phase, less protein is found at the furrow canal, and the majority of nullo protein is dispersed in the cytoplasm. Protein is not detected above background levels at the completion of cellularization.
GBrowse - Visual display of RNA-Seq signalsView Dmel\nullo in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
dsRNA has been made from templates generated with primers directed against this gene.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
nullo loss of function mutations cause defects in the formation and maintenance of the basal junction, but do not affect apical junction formation during cellularisation in the embryo.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
The requirement for nullo function during development has been determined. Analysis of nullo mutant clones in adults reveals that nullo activity is not required for cell division in imaginal discs. Germline clone experiments suggest that maternal expression of nullo is not essential for either germline proliferation or the cellularisation of progeny.
The nullo gene is required for the stability of the actin-myosin contractile network during cellularization.